Prupe.1G221700_v2.0.a1

Amidohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0015263
Physical Location & Seq
Reverse (-)
23604826 .. 23607882
3057 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G221700.1

Sequence Viewer

Length: 1050 bp
ATGAAAGGGTCTTTTAGCCATAATCCAAAGGCTATGGCAGTCAGGCTAACGCCTCTTCTGCTTTCAGCTTCAGCTCAAGTCCCTGTAGTGCAACTTAAATGTCCACCCCCATTTGCCCACATTTCAACAAAGACCCGGTTCATAGTCTCTTCTGCAAGAATGGCTACCAATGAGCCCCAGAGTAGAACCAAGATCATAGATTCACATCTGCATGTGTGGGCATCTCCCCAAGAGGCTGCGGATAAATACCCTTACTTTCCTGGCCAAGAACCTACTTTGCCTGGAGATGTCGATTTCTTGCTCAAGTCTATGGAAGAAGCAGGTGTTGACGGTGCGCTTATTGTGCAACCCATTAATCACAAATTCGATCATTCTTTGGTGACAAGTGTGTTGAAGAAGTATCCATCTAAATTTGTTGGTTGTTGCCTAGCAAATCCAACCGAAGATGGAAGTGGGGTTAAGCAACTTGAACGTCTCATTCTGCAGGATAATTATCGTGCTGTTCGCTTCAATCCATATCTGTGGCCTTCTGGTCAACAGATGACCAATGAAGTTGGGAAGGCAATGTTCTCCAAGGCAGGAGAACTTGGAGTACCAGTTGGTTTTATGTGTATGAAGGGCCTCAGTCTGCATATTTCAGAAATTGAGGAGCTGTGCACAGAGTTTCCATCAACAACTGTATTGCTTGATCATTTGGCTTTCTGCAAACCACCAATAAACGATGAGGAAAAGCTGGCTTTCTCTGCACTTTTAAAGCTATCCAGATTCCCACAGGTCTATGTGAAATTCAGTGCACTTTTCAGGGTGTCAAGAATGCCATTTCCATATCAGGATTTATCTCACTCACTGTCTCAAGTTGTCTCGAGCTTTGGTGCTAACCATGTCATGTGGGGCAGTGATTTTCCATTTGTTGTTCCCGAGTGTGGTTATAAAGGAGCGAAAGAAGCCATTTCACTTATCGTCAATCAAATACCTCTATCATCTACAGACTTGGAGTGGATCATGGGAAGGACGGTCATGCAGCTCTTTCAAAATCAATGGCTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_2

Protein Analysis

350

Amino Acids

38.84

Weight (kDa)

7.64

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35450
fragaria_vesca FvH4_4g23290 FvH4_4g23290
malus_domestica MD16G1123900.v1.1
prunus_persica Prupe.1G221700_v2.0.a1
pyrus_communis pycom16g10590
rosa_chinensis RchiOBHm_Chr4g0429651
rosa_laevigata RLG00000007055
rosa_multiflora Rmu_sc0006590.1_g000006
rosa_roxburghii Rroxscaffold_5G00371330
rosa_rugosa Rorug04G0237500
rosa_samantha Rh4AG293500 Rh4BG299300 Rh4CG315100 Rh4DG296400
rosa_wichuraiana Rw4G025400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 930
AarI CACCTGC 1 cut(s) 311
Acc36I ACCTGC 1 cut(s) 311
AciI CCGC 1 cut(s) 239
AclWI GGATC 1 cut(s) 1007
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 3 cut(s) 362, 410, 785
AcuI CTGAAG 1 cut(s) 54
AfaI GTAC 1 cut(s) 594
AfiI CCNNNNNNNGG 1 cut(s) 923
AgsI TTSAA 5 cut(s) 126, 394, 470, 511, 1031
AjnI CCWGG 2 cut(s) 259, 280
AluBI AGCT 7 cut(s) 68, 74, 652, 733, 757, 867, 1024
AluI AGCT 7 cut(s) 68, 74, 652, 733, 757, 867, 1024
Alw21I GWGCWC 2 cut(s) 659, 796
Alw26I GTCTC 4 cut(s) 151, 479, 855, 865
Alw44I GTGCAC 2 cut(s) 655, 792
AlwI GGATC 1 cut(s) 1007
Ama87I CYCGRG 2 cut(s) 862, 917
AoxI GGCC 3 cut(s) 262, 524, 619
ApaLI GTGCAC 2 cut(s) 655, 792
ApeKI GCWGC 2 cut(s) 236, 1021
ApoI RAATTY 3 cut(s) 362, 410, 785
AseI ATTAAT 1 cut(s) 354
AspLEI GCGC 1 cut(s) 337
AspS9I GGNCC 1 cut(s) 619
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 1 cut(s) 391
AvaI CYCGRG 2 cut(s) 862, 917
BaeGI GKGCMC 2 cut(s) 659, 796
BalI TGGCCA 1 cut(s) 264
BanII GRGCYC 1 cut(s) 177
Bbv12I GWGCWC 2 cut(s) 659, 796
BbvI GCAGC 2 cut(s) 223, 1033
BccI CCATC 3 cut(s) 412, 440, 676
BciT130I CCWGG 2 cut(s) 261, 282
BciVI GTATCC 1 cut(s) 411
BclI TGATCA 1 cut(s) 688
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 4 cut(s) 151, 479, 855, 865
BfaI CTAG 1 cut(s) 428
BfmI CTRYAG 3 cut(s) 84, 482, 984
BfuAI ACCTGC 1 cut(s) 311
BfuI GTATCC 1 cut(s) 411
BisI GCNGC 2 cut(s) 237, 1022
BlsI GCNGC 2 cut(s) 238, 1023
Bme1390I CCNGG 3 cut(s) 136, 261, 282
BmeT110I CYCGRG 2 cut(s) 862, 917
BmgT120I GGNCC 1 cut(s) 619
BmrFI CCNGG 3 cut(s) 136, 261, 282
BmsI GCATC 1 cut(s) 230
BpmI CTGGAG 1 cut(s) 303
BpuEI CTTGAG 3 cut(s) 60, 287, 837
BpuMI CCSGG 1 cut(s) 136
BsaBI GATNNNNATC 2 cut(s) 204, 492
BsaJI CCNNGG 1 cut(s) 573
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 1 cut(s) 923
Bse1I ACTGG 1 cut(s) 596
Bse3DI GCAATG 1 cut(s) 570
Bse8I GATNNNNATC 2 cut(s) 204, 492
BseBI CCWGG 2 cut(s) 261, 282
BseDI CCNNGG 1 cut(s) 573
BseJI GATNNNNATC 2 cut(s) 204, 492
BseLI CCNNNNNNNGG 1 cut(s) 923
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 1 cut(s) 637
BseNI ACTGG 1 cut(s) 596
BseRI GAGGAG 1 cut(s) 662
BseSI GKGCMC 2 cut(s) 659, 796
BseXI GCAGC 2 cut(s) 223, 1033
BsgI GTGCAG 1 cut(s) 729
BshFI GGCC 3 cut(s) 264, 526, 621
BsiHKAI GWGCWC 2 cut(s) 659, 796
BsiHKCI CYCGRG 2 cut(s) 862, 917
BsiSI CCGG 1 cut(s) 136
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 1 cut(s) 923
BsmAI GTCTC 4 cut(s) 151, 479, 855, 865
BsmBI CGTCTC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 65
BsmI GAATGC 1 cut(s) 819
BsnI GGCC 3 cut(s) 264, 526, 621
BsoBI CYCGRG 2 cut(s) 862, 917
Bsp1286I GDGCHC 3 cut(s) 177, 659, 796
Bsp143I GATC 4 cut(s) 192, 367, 688, 999
BspACI CCGC 1 cut(s) 239
BspANI GGCC 3 cut(s) 264, 526, 621
BspCNI CTCAG 1 cut(s) 636
BspMAI CTGCAG 1 cut(s) 486
BspMI ACCTGC 1 cut(s) 311
BspPI GGATC 1 cut(s) 1007
BsrDI GCAATG 1 cut(s) 570
BsrI ACTGG 1 cut(s) 596
BssECI CCNNGG 1 cut(s) 573
BssMI GATC 4 cut(s) 192, 367, 688, 999
BssT1I CCWWGG 1 cut(s) 573
Bst2UI CCWGG 2 cut(s) 261, 282
Bst4CI ACNGT 4 cut(s) 332, 679, 849, 1015
Bst6I CTCTTC 2 cut(s) 60, 154
BstC8I GCNNGC 1 cut(s) 735
BstDEI CTNAG 1 cut(s) 623
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 4 cut(s) 195, 370, 691, 1002
BstMAI GTCTC 4 cut(s) 151, 479, 855, 865
BstMBI GATC 4 cut(s) 192, 367, 688, 999
BstMWI GCNNNNNNNGC 5 cut(s) 58, 161, 343, 743, 944
BstNI CCWGG 2 cut(s) 261, 282
BstNSI RCATGY 1 cut(s) 215
BstSCI CCNGG 3 cut(s) 134, 259, 280
BstSFI CTRYAG 3 cut(s) 84, 482, 984
BstSLI GKGCMC 2 cut(s) 659, 796
BstV1I GCAGC 2 cut(s) 223, 1033
BstXI CCANNNNNNTGG 1 cut(s) 522
BsuI GTATCC 1 cut(s) 411
BsuRI GGCC 3 cut(s) 264, 526, 621
BtsI GCAGTG 1 cut(s) 901
BtsIMutI CAGTG 3 cut(s) 796, 845, 901
BveI ACCTGC 1 cut(s) 311
Cac8I GCNNGC 1 cut(s) 735
CfoI GCGC 1 cut(s) 337
Cfr13I GGNCC 1 cut(s) 619
Csp6I GTAC 1 cut(s) 593
CviAII CATG 5 cut(s) 212, 881, 886, 1003, 1018
CviQI GTAC 1 cut(s) 593
DdeI CTNAG 1 cut(s) 623
DpnI GATC 4 cut(s) 194, 369, 690, 1001
DpnII GATC 4 cut(s) 192, 367, 688, 999
DraI TTTAAA 1 cut(s) 753
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 2 cut(s) 60, 154
EarI CTCTTC 2 cut(s) 60, 154
Eco130I CCWWGG 1 cut(s) 573
Eco24I GRGCYC 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 54
Eco88I CYCGRG 2 cut(s) 862, 917
EcoO109I RGGNCCY 1 cut(s) 619
EcoRII CCWGG 2 cut(s) 259, 280
EcoT14I CCWWGG 1 cut(s) 573
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 573
Esp3I CGTCTC 1 cut(s) 479
FaeI CATG 5 cut(s) 215, 884, 889, 1006, 1021
FaqI GGGAC 1 cut(s) 65
FatI CATG 5 cut(s) 211, 880, 885, 1002, 1017
FbaI TGATCA 1 cut(s) 688
Fnu4HI GCNGC 2 cut(s) 237, 1022
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 2 cut(s) 237, 1022
FspBI CTAG 1 cut(s) 428
GlaI GCGC 1 cut(s) 336
GluI GCNGC 2 cut(s) 237, 1022
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 3 cut(s) 264, 526, 621
HapII CCGG 1 cut(s) 136
HhaI GCGC 1 cut(s) 337
Hin1II CATG 5 cut(s) 215, 884, 889, 1006, 1021
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HincII GTYRAC 2 cut(s) 328, 536
HindII GTYRAC 2 cut(s) 328, 536
HinfI GANTC 2 cut(s) 200, 765
HpaII CCGG 1 cut(s) 136
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 5 cut(s) 104, 328, 536, 657, 794
Hpy188I TCNGA 1 cut(s) 640
Hpy188III TCNNGA 5 cut(s) 762, 810, 830, 862, 917
Hpy8I GTNNAC 5 cut(s) 104, 328, 536, 657, 794
HpyAV CCTTC 4 cut(s) 537, 553, 610, 1002
HpyCH4III ACNGT 4 cut(s) 332, 679, 849, 1015
HpyCH4IV ACGT 1 cut(s) 472
HpyF10VI GCNNNNNNNGC 5 cut(s) 58, 161, 343, 743, 944
HpyF3I CTNAG 1 cut(s) 623
HpySE526I ACGT 1 cut(s) 472
Hsp92II CATG 5 cut(s) 215, 884, 889, 1006, 1021
HspAI GCGC 1 cut(s) 335
Ksp22I TGATCA 1 cut(s) 688
Kzo9I GATC 4 cut(s) 192, 367, 688, 999
LmnI GCTCC 2 cut(s) 649, 935
Lsp1109I GCAGC 2 cut(s) 223, 1033
LweI GCATC 1 cut(s) 230
MaeI CTAG 1 cut(s) 428
MaeII ACGT 1 cut(s) 472
MaeIII GTNAC 1 cut(s) 379
MalI GATC 4 cut(s) 194, 369, 690, 1001
MboI GATC 4 cut(s) 192, 367, 688, 999
MboII GAAGA 5 cut(s) 47, 141, 326, 406, 455
MhlI GDGCHC 3 cut(s) 177, 659, 796
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 5 cut(s) 362, 410, 490, 642, 785
MluNI TGGCCA 1 cut(s) 264
MmeI TCCRAC 1 cut(s) 461
MnlI CCTC 6 cut(s) 63, 226, 632, 640, 718, 984
Mox20I TGGCCA 1 cut(s) 264
MscI TGGCCA 1 cut(s) 264
MseI TTAA 4 cut(s) 96, 354, 459, 752
MslI CAYNNNNRTG 2 cut(s) 210, 520
Msp20I TGGCCA 1 cut(s) 264
MspI CCGG 1 cut(s) 136
MspR9I CCNGG 3 cut(s) 136, 261, 282
Mva1269I GAATGC 1 cut(s) 819
MvaI CCWGG 2 cut(s) 261, 282
MwoI GCNNNNNNNGC 5 cut(s) 58, 161, 343, 743, 944
NciI CCSGG 1 cut(s) 136
NdeII GATC 4 cut(s) 192, 367, 688, 999
NlaIII CATG 5 cut(s) 215, 884, 889, 1006, 1021
NmuCI GTSAC 1 cut(s) 379
NspI RCATGY 1 cut(s) 215
PaeR7I CTCGAG 1 cut(s) 862
PaqCI CACCTGC 1 cut(s) 311
PctI GAATGC 1 cut(s) 819
PfeI GAWTC 2 cut(s) 200, 765
PkrI GCNGC 2 cut(s) 238, 1023
PshBI ATTAAT 1 cut(s) 354
PsiI TTATAA 1 cut(s) 930
Psp6I CCWGG 2 cut(s) 259, 280
PspGI CCWGG 2 cut(s) 259, 280
PspPI GGNCC 1 cut(s) 619
PsrI GAACNNNNNNTAC 2 cut(s) 576, 608
PstI CTGCAG 1 cut(s) 486
RsaI GTAC 1 cut(s) 594
RsaNI GTAC 1 cut(s) 593
RseI CAYNNNNRTG 2 cut(s) 210, 520
SaqAI TTAA 4 cut(s) 96, 354, 459, 752
SatI GCNGC 2 cut(s) 237, 1022
Sau3AI GATC 4 cut(s) 192, 367, 688, 999
Sau96I GGNCC 1 cut(s) 619
ScrFI CCNGG 3 cut(s) 136, 261, 282
SduI GDGCHC 3 cut(s) 177, 659, 796
SfaNI GCATC 1 cut(s) 230
SfcI CTRYAG 3 cut(s) 84, 482, 984
Sfr274I CTCGAG 1 cut(s) 862
SlaI CTCGAG 1 cut(s) 862
SmiMI CAYNNNNRTG 2 cut(s) 210, 520
SmlI CTYRAG 4 cut(s) 75, 302, 852, 862
SmoI CTYRAG 4 cut(s) 75, 302, 852, 862
Sse9I AATT 5 cut(s) 362, 410, 490, 642, 785
SsiI CCGC 1 cut(s) 239
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 3 cut(s) 134, 259, 280
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 4 cut(s) 332, 679, 849, 1015
TaiI ACGT 1 cut(s) 475
TaqI TCGA 3 cut(s) 291, 366, 863
TasI AATT 5 cut(s) 362, 410, 490, 642, 785
TfiI GAWTC 2 cut(s) 200, 765
Tru1I TTAA 4 cut(s) 96, 354, 459, 752
Tru9I TTAA 4 cut(s) 96, 354, 459, 752
TscAI CASTG 3 cut(s) 796, 852, 901
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 2 cut(s) 236, 1021
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 4 cut(s) 17, 130, 564, 629
TspRI CASTG 3 cut(s) 796, 852, 901
VneI GTGCAC 2 cut(s) 655, 792
VspI ATTAAT 1 cut(s) 354
XapI RAATTY 3 cut(s) 362, 410, 785
XceI RCATGY 1 cut(s) 215
XhoI CTCGAG 1 cut(s) 862
XspI CTAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.