RLG00000007055

Amidohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0015263
Physical Location & Seq
Forward (+)
13122037 .. 13124925
2889 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007055

Sequence Viewer

Length: 993 bp
ATGGCAGTGAGGCTAACACCTGTTATGGTTCCAGCTTCAGCTAAGTTTAAACACTCTGCCTTCAGTTCAAGAAAAACCCAGTTCAGGGTCTCTGCTGCAGCAATGGCTACAGGTGGACCACAGAGCAGAACTAAAGTCGTAGATTCACATCTCCATGTGTGGGCTTCACCTCAAGAGGCGGCTGATAAATATCCTTACTTTCCTGGGCAAGAACCTACTTTGCCTGGAGATGTCGATTTATTGCTCAAGTGTATGGAAGAAGCAGGTGTTGACGGTGCACTTATTGTGCAGCCTATTAATCACAAATTCGATCATTCTTTGGTGACAAGTGTGTTGAAGAAGTATCCATCTAAATTTGTTGGTTGTTGCCTTGCAAATCCAGCTGAAGATGGGAGTGGGGTTAAGCAGCTTGAACATCTTATTTTAAAGGATAACTATCGTGCTGTTCGCTTCAATCCATATTTATGGCCTTCTGGTCAAAAGATGACCAATGAAGTTGGAAAGGCAATGTTCTCTAAGGCGGGAGAACTTGGAGTACCAGTTGGCTTTATGTGTATGAAGGGCCTCGGTCTGCATATTTCAGAAATTGAGGAACTATGCACAGAGTCTCCTTCAACACTTGTATTACTTGATCATTTGGCTTTCTGCAAACCACCAATAAATGATGAGGAAAATGAGGCTTTCTCTGCACTTTTAAAGCTATCCAGATTTCCACAGGTCTATGTAAAAGTAAGTGCACTTTTCAGGGTGTCAAGAACGCCATTTCCATATGAAGATGTATCTCACACACTGTCCCAAGTTGTGTCAAGTTTTGGTGCTAACCGTGTCATGTGGGGCAGTGATTTTCCATTTGTTGTTGCTGAGTGTGGTTATAAAGGAGCAAAAGAAGCCGTTTCTTTGATTGCCAGTAAAATACCTCTATCATCTTCAGAGTTGGAGTGGATCATGGGTAGAACAATCTTGCAACTCTTCCAGAATCAGTGGCTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_2

Protein Analysis

331

Amino Acids

36.34

Weight (kDa)

7.62

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_2 PF04909 47 - 324 1.1e-28 Amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35450
fragaria_vesca FvH4_4g23290 FvH4_4g23290
malus_domestica MD16G1123900.v1.1
prunus_persica Prupe.1G221700_v2.0.a1
pyrus_communis pycom16g10590
rosa_chinensis RchiOBHm_Chr4g0429651
rosa_laevigata RLG00000007055
rosa_multiflora Rmu_sc0006590.1_g000006
rosa_roxburghii Rroxscaffold_5G00371330
rosa_rugosa Rorug04G0237500
rosa_samantha Rh4AG293500 Rh4BG299300 Rh4CG315100 Rh4DG296400
rosa_wichuraiana Rw4G025400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 873
AarI CACCTGC 1 cut(s) 254
Acc36I ACCTGC 1 cut(s) 254
AciI CCGC 2 cut(s) 179, 521
AclWI GGATC 1 cut(s) 950
AcsI RAATTY 2 cut(s) 305, 353
AcuI CTGAAG 4 cut(s) 21, 46, 405, 912
AfaI GTAC 1 cut(s) 537
AfiI CCNNNNNNNGG 3 cut(s) 84, 85, 160
AgsI TTSAA 5 cut(s) 69, 337, 413, 454, 615
AjnI CCWGG 2 cut(s) 202, 223
AloI GAACNNNNNNTCC 2 cut(s) 748, 780
AluBI AGCT 5 cut(s) 35, 41, 383, 409, 700
AluI AGCT 5 cut(s) 35, 41, 383, 409, 700
Alw21I GWGCWC 2 cut(s) 280, 739
Alw26I GTCTC 2 cut(s) 94, 612
Alw44I GTGCAC 2 cut(s) 276, 735
AlwI GGATC 1 cut(s) 950
AoxI GGCC 2 cut(s) 467, 562
ApaLI GTGCAC 2 cut(s) 276, 735
ApeKI GCWGC 4 cut(s) 95, 98, 289, 406
ApoI RAATTY 2 cut(s) 305, 353
AseI ATTAAT 1 cut(s) 297
AspS9I GGNCC 2 cut(s) 116, 562
AsuHPI GGTGA 2 cut(s) 159, 334
AvaII GGWCC 1 cut(s) 116
BaeGI GKGCMC 2 cut(s) 280, 739
Bbv12I GWGCWC 2 cut(s) 280, 739
BbvI GCAGC 4 cut(s) 82, 110, 301, 418
BccI CCATC 2 cut(s) 355, 383
BceAI ACGGC 1 cut(s) 875
BciT130I CCWGG 2 cut(s) 204, 225
BciVI GTATCC 1 cut(s) 354
BclI TGATCA 1 cut(s) 631
BcoDI GTCTC 2 cut(s) 94, 612
BfmI CTRYAG 2 cut(s) 96, 108
BfuAI ACCTGC 1 cut(s) 254
BfuI GTATCC 1 cut(s) 354
BisI GCNGC 5 cut(s) 96, 99, 180, 290, 407
BlsI GCNGC 5 cut(s) 97, 100, 181, 291, 408
Bme1390I CCNGG 2 cut(s) 204, 225
Bme18I GGWCC 1 cut(s) 116
BmgT120I GGNCC 2 cut(s) 116, 562
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 204, 225
BmrI ACTGGG 1 cut(s) 73
BmuI ACTGGG 1 cut(s) 73
BplI GAGNNNNNCTC 2 cut(s) 668, 700
BpmI CTGGAG 1 cut(s) 246
BpuEI CTTGAG 2 cut(s) 156, 230
BsaBI GATNNNNATC 3 cut(s) 147, 189, 435
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 2 cut(s) 203, 565
BsaXI ACNNNNNCTCC 4 cut(s) 525, 555, 592, 622
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 85, 160
Bse1I ACTGG 3 cut(s) 79, 539, 906
Bse3DI GCAATG 2 cut(s) 108, 513
Bse8I GATNNNNATC 3 cut(s) 147, 189, 435
BseBI CCWGG 2 cut(s) 204, 225
BseDI CCNNGG 2 cut(s) 203, 565
BseJI GATNNNNATC 3 cut(s) 147, 189, 435
BseLI CCNNNNNNNGG 3 cut(s) 84, 85, 160
BseMI GCAATG 2 cut(s) 108, 513
BseMII CTCAG 1 cut(s) 852
BseNI ACTGG 3 cut(s) 79, 539, 906
BseSI GKGCMC 2 cut(s) 280, 739
BseXI GCAGC 4 cut(s) 82, 110, 301, 418
BsgI GTGCAG 2 cut(s) 308, 672
BshFI GGCC 2 cut(s) 469, 564
BsiHKAI GWGCWC 2 cut(s) 280, 739
BslFI GGGAC 1 cut(s) 778
BslI CCNNNNNNNGG 3 cut(s) 84, 85, 160
BsmAI GTCTC 2 cut(s) 94, 612
BsmFI GGGAC 1 cut(s) 778
BsnI GGCC 2 cut(s) 469, 564
Bso31I GGTCTC 1 cut(s) 94
Bsp1286I GDGCHC 2 cut(s) 280, 739
Bsp143I GATC 3 cut(s) 310, 631, 942
BspACI CCGC 2 cut(s) 179, 521
BspANI GGCC 2 cut(s) 469, 564
BspCNI CTCAG 1 cut(s) 853
BspLI GGNNCC 1 cut(s) 30
BspMAI CTGCAG 1 cut(s) 100
BspMI ACCTGC 1 cut(s) 254
BspPI GGATC 1 cut(s) 950
BspTNI GGTCTC 1 cut(s) 94
BsrDI GCAATG 2 cut(s) 108, 513
BsrI ACTGG 3 cut(s) 79, 539, 906
BssECI CCNNGG 2 cut(s) 203, 565
BssMI GATC 3 cut(s) 310, 631, 942
Bst2UI CCWGG 2 cut(s) 204, 225
Bst4CI ACNGT 3 cut(s) 275, 792, 824
Bst6I CTCTTC 1 cut(s) 974
BstDEI CTNAG 3 cut(s) 42, 516, 861
BstKTI GATC 3 cut(s) 313, 634, 945
BstMAI GTCTC 2 cut(s) 94, 612
BstMBI GATC 3 cut(s) 310, 631, 942
BstMWI GCNNNNNNNGC 4 cut(s) 104, 380, 686, 887
BstNI CCWGG 2 cut(s) 204, 225
BstSCI CCNGG 2 cut(s) 202, 223
BstSFI CTRYAG 2 cut(s) 96, 108
BstSLI GKGCMC 2 cut(s) 280, 739
BstV1I GCAGC 4 cut(s) 82, 110, 301, 418
BstXI CCANNNNNNTGG 1 cut(s) 465
BsuI GTATCC 1 cut(s) 354
BsuRI GGCC 2 cut(s) 469, 564
BtsI GCAGTG 2 cut(s) 12, 844
BtsIMutI CAGTG 4 cut(s) 12, 788, 844, 986
BveI ACCTGC 1 cut(s) 254
Cfr13I GGNCC 2 cut(s) 116, 562
Csp6I GTAC 1 cut(s) 536
CviAII CATG 3 cut(s) 155, 829, 946
CviQI GTAC 1 cut(s) 536
DdeI CTNAG 3 cut(s) 42, 516, 861
DpnI GATC 3 cut(s) 312, 633, 944
DpnII GATC 3 cut(s) 310, 631, 942
DraI TTTAAA 3 cut(s) 49, 426, 696
Eam1104I CTCTTC 1 cut(s) 974
EarI CTCTTC 1 cut(s) 974
Eco31I GGTCTC 1 cut(s) 94
Eco47I GGWCC 1 cut(s) 116
Eco57I CTGAAG 4 cut(s) 21, 46, 405, 912
EcoO109I RGGNCCY 1 cut(s) 562
EcoRII CCWGG 2 cut(s) 202, 223
FaeI CATG 3 cut(s) 158, 832, 949
FaqI GGGAC 1 cut(s) 778
FatI CATG 3 cut(s) 154, 828, 945
FauI CCCGC 1 cut(s) 514
FauNDI CATATG 1 cut(s) 769
FbaI TGATCA 1 cut(s) 631
Fnu4HI GCNGC 5 cut(s) 96, 99, 180, 290, 407
Fsp4HI GCNGC 5 cut(s) 96, 99, 180, 290, 407
GluI GCNGC 5 cut(s) 96, 99, 180, 290, 407
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 2 cut(s) 469, 564
Hin1II CATG 3 cut(s) 158, 832, 949
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 3 cut(s) 143, 605, 976
HphI GGTGA 2 cut(s) 159, 334
Hpy166II GTNNAC 4 cut(s) 116, 271, 278, 737
Hpy188I TCNGA 2 cut(s) 583, 931
Hpy188III TCNNGA 5 cut(s) 69, 173, 705, 753, 973
Hpy8I GTNNAC 4 cut(s) 116, 271, 278, 737
HpyAV CCTTC 4 cut(s) 70, 480, 553, 621
HpyCH4III ACNGT 3 cut(s) 275, 792, 824
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 380, 686, 887
HpyF3I CTNAG 3 cut(s) 42, 516, 861
Hsp92II CATG 3 cut(s) 158, 832, 949
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 3 cut(s) 310, 631, 942
LmnI GCTCC 1 cut(s) 878
Lsp1109I GCAGC 4 cut(s) 82, 110, 301, 418
MaeIII GTNAC 1 cut(s) 322
MalI GATC 3 cut(s) 312, 633, 944
MboI GATC 3 cut(s) 310, 631, 942
MboII GAAGA 6 cut(s) 269, 349, 398, 785, 918, 961
MhlI GDGCHC 2 cut(s) 280, 739
MluCI AATT 3 cut(s) 305, 353, 585
MlyI GAGTC 1 cut(s) 614
MmeI TCCRAC 2 cut(s) 478, 915
MnlI CCTC 8 cut(s) 3, 169, 180, 575, 583, 661, 670, 927
MseI TTAA 5 cut(s) 48, 297, 402, 425, 695
MslI CAYNNNNRTG 2 cut(s) 153, 463
MspA1I CMGCKG 1 cut(s) 383
MspR9I CCNGG 2 cut(s) 204, 225
MssI GTTTAAAC 1 cut(s) 49
MvaI CCWGG 2 cut(s) 204, 225
MwoI GCNNNNNNNGC 4 cut(s) 104, 380, 686, 887
NdeI CATATG 1 cut(s) 769
NdeII GATC 3 cut(s) 310, 631, 942
NlaIII CATG 3 cut(s) 158, 832, 949
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 322
PaqCI CACCTGC 1 cut(s) 254
PfeI GAWTC 2 cut(s) 143, 976
PkrI GCNGC 5 cut(s) 97, 100, 181, 291, 408
PleI GAGTC 1 cut(s) 613
PmeI GTTTAAAC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 613
PshBI ATTAAT 1 cut(s) 297
PsiI TTATAA 1 cut(s) 873
Psp6I CCWGG 2 cut(s) 202, 223
PspGI CCWGG 2 cut(s) 202, 223
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 2 cut(s) 116, 562
PsrI GAACNNNNNNTAC 2 cut(s) 519, 551
PstI CTGCAG 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 1 cut(s) 537
RsaNI GTAC 1 cut(s) 536
RseI CAYNNNNRTG 2 cut(s) 153, 463
SaqAI TTAA 5 cut(s) 48, 297, 402, 425, 695
SatI GCNGC 5 cut(s) 96, 99, 180, 290, 407
Sau3AI GATC 3 cut(s) 310, 631, 942
Sau96I GGNCC 2 cut(s) 116, 562
SchI GAGTC 1 cut(s) 614
ScrFI CCNGG 2 cut(s) 204, 225
SduI GDGCHC 2 cut(s) 280, 739
SfcI CTRYAG 2 cut(s) 96, 108
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 2 cut(s) 153, 463
SmlI CTYRAG 2 cut(s) 171, 245
SmoI CTYRAG 2 cut(s) 171, 245
Sse9I AATT 3 cut(s) 305, 353, 585
SsiI CCGC 2 cut(s) 179, 521
StyD4I CCNGG 2 cut(s) 202, 223
TaaI ACNGT 3 cut(s) 275, 792, 824
TaqI TCGA 2 cut(s) 234, 309
TaqII GACCGA 1 cut(s) 557
TasI AATT 3 cut(s) 305, 353, 585
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 2 cut(s) 143, 976
Tru1I TTAA 5 cut(s) 48, 297, 402, 425, 695
Tru9I TTAA 5 cut(s) 48, 297, 402, 425, 695
TscAI CASTG 4 cut(s) 12, 795, 844, 986
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 4 cut(s) 95, 98, 289, 406
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 3 cut(s) 507, 572, 786
TspRI CASTG 4 cut(s) 12, 795, 844, 986
VneI GTGCAC 2 cut(s) 276, 735
VpaK11BI GGWCC 1 cut(s) 116
VspI ATTAAT 1 cut(s) 297
XapI RAATTY 2 cut(s) 305, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.