Rh4AG293500

Amidohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0015263
Physical Location & Seq
Reverse (-)
60704434 .. 60706764
2331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG293500.1

Sequence Viewer

Length: 741 bp
ATGGAAGAAGCAGGTGTTGACGGTGCACTTATTGTGCAGCCTATTAATCACAAATTCGATCACTCTTTGGTGACAAGTGTGTTGAAGAAGTATCCATCTAAATTTGTTGGTTGTTGCCTTGCAAATCCAGCTGAAGATGGGAGTGGGGTTAAGCAGCTTGAACATCTTATTTTAAAGGATAACTATCGTGCTGTTCGCTTCAATCCATATTTATGGCCATCTGGTCAAAAGATGACCAATGAAGTTGGAAAGGCAATGTTCTCTAAGGCGGGAGAACTTGGAGTACCAGTTGGCTTTATGTGTATGAAGGGCCTCGGTCTGCATATTTCAGAAATTGAGGAACTATGCACAGAGTTTCCTTCAACACTTGTATTACTTGATCATTTGGCTTTCTGCAAACCACCAATAAATGATGAGGAAAATGAGGCTTTCTCTGCACTTTTAAAGCTATCCAGATTTCCACAGGTCTATGTAAAAGTAAGTGCACTTTTCAGGGTGTCAAGAACGCCATTTCCATATGAAGATGTATCTCACACACTGTCCCAAGTTGTGTCAAGTTTTGGTGCTAACCGTGTCATGTGGGGCAGTGATTTTCCATTTGTTGTTGCTGAGTGTGGTTATAAAGGAGCAAAAGAAGCCGTTTCTTTGATTGCCAGTAAAATACCTCTATCATCTTCAGAGTTGGAGTGGATCATGGGTAGAACAATCTTGCAACTCTTCCAGAATCAGTGGCTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_2

Protein Analysis

246

Amino Acids

27.31

Weight (kDa)

6.09

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_2 PF04909 4 - 240 7.2e-26 Amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35450
fragaria_vesca FvH4_4g23290 FvH4_4g23290
malus_domestica MD16G1123900.v1.1
prunus_persica Prupe.1G221700_v2.0.a1
pyrus_communis pycom16g10590
rosa_chinensis RchiOBHm_Chr4g0429651
rosa_laevigata RLG00000007055
rosa_multiflora Rmu_sc0006590.1_g000006
rosa_roxburghii Rroxscaffold_5G00371330
rosa_rugosa Rorug04G0237500
rosa_samantha Rh4AG293500 Rh4BG299300 Rh4CG315100 Rh4DG296400
rosa_wichuraiana Rw4G025400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
AarI CACCTGC 1 cut(s) 2
Acc36I ACCTGC 1 cut(s) 2
AciI CCGC 1 cut(s) 269
AclWI GGATC 1 cut(s) 698
AcoI YGGCCR 1 cut(s) 215
AcsI RAATTY 2 cut(s) 53, 101
AcuI CTGAAG 2 cut(s) 153, 660
AfaI GTAC 1 cut(s) 285
AgsI TTSAA 4 cut(s) 85, 161, 202, 363
AloI GAACNNNNNNTCC 2 cut(s) 496, 528
AluBI AGCT 3 cut(s) 131, 157, 448
AluI AGCT 3 cut(s) 131, 157, 448
Alw21I GWGCWC 2 cut(s) 28, 487
Alw44I GTGCAC 2 cut(s) 24, 483
AlwI GGATC 1 cut(s) 698
AoxI GGCC 2 cut(s) 215, 310
ApaLI GTGCAC 2 cut(s) 24, 483
ApeKI GCWGC 2 cut(s) 37, 154
ApoI RAATTY 2 cut(s) 53, 101
AseI ATTAAT 1 cut(s) 45
AspS9I GGNCC 1 cut(s) 310
AsuHPI GGTGA 1 cut(s) 82
BaeGI GKGCMC 2 cut(s) 28, 487
BalI TGGCCA 1 cut(s) 217
Bbv12I GWGCWC 2 cut(s) 28, 487
BbvI GCAGC 2 cut(s) 49, 166
BccI CCATC 3 cut(s) 103, 131, 226
BceAI ACGGC 1 cut(s) 623
BciVI GTATCC 1 cut(s) 102
BclI TGATCA 1 cut(s) 379
BfuAI ACCTGC 1 cut(s) 2
BfuI GTATCC 1 cut(s) 102
BisI GCNGC 2 cut(s) 38, 155
BlsI GCNGC 2 cut(s) 39, 156
BmgT120I GGNCC 1 cut(s) 310
BplI GAGNNNNNCTC 2 cut(s) 416, 448
BsaBI GATNNNNATC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 313
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bse1I ACTGG 2 cut(s) 287, 654
Bse3DI GCAATG 1 cut(s) 261
Bse8I GATNNNNATC 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 313
BseJI GATNNNNATC 1 cut(s) 183
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 600
BseNI ACTGG 2 cut(s) 287, 654
BseSI GKGCMC 2 cut(s) 28, 487
BseXI GCAGC 2 cut(s) 49, 166
BsgI GTGCAG 2 cut(s) 56, 420
BshFI GGCC 2 cut(s) 217, 312
BsiHKAI GWGCWC 2 cut(s) 28, 487
BslFI GGGAC 1 cut(s) 526
BsmFI GGGAC 1 cut(s) 526
BsnI GGCC 2 cut(s) 217, 312
Bsp1286I GDGCHC 2 cut(s) 28, 487
Bsp143I GATC 3 cut(s) 58, 379, 690
BspACI CCGC 1 cut(s) 269
BspANI GGCC 2 cut(s) 217, 312
BspCNI CTCAG 1 cut(s) 601
BspMI ACCTGC 1 cut(s) 2
BspPI GGATC 1 cut(s) 698
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 2 cut(s) 287, 654
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 3 cut(s) 58, 379, 690
Bst4CI ACNGT 3 cut(s) 23, 540, 572
Bst6I CTCTTC 1 cut(s) 722
BstDEI CTNAG 2 cut(s) 264, 609
BstKTI GATC 3 cut(s) 61, 382, 693
BstMBI GATC 3 cut(s) 58, 379, 690
BstMWI GCNNNNNNNGC 3 cut(s) 128, 434, 635
BstSLI GKGCMC 2 cut(s) 28, 487
BstV1I GCAGC 2 cut(s) 49, 166
BstXI CCANNNNNNTGG 1 cut(s) 213
BsuI GTATCC 1 cut(s) 102
BsuRI GGCC 2 cut(s) 217, 312
BtsI GCAGTG 1 cut(s) 592
BtsIMutI CAGTG 3 cut(s) 536, 592, 734
BveI ACCTGC 1 cut(s) 2
Cfr13I GGNCC 1 cut(s) 310
Csp6I GTAC 1 cut(s) 284
CviAII CATG 2 cut(s) 577, 694
CviQI GTAC 1 cut(s) 284
DdeI CTNAG 2 cut(s) 264, 609
DpnI GATC 3 cut(s) 60, 381, 692
DpnII GATC 3 cut(s) 58, 379, 690
DraI TTTAAA 2 cut(s) 174, 444
EaeI YGGCCR 1 cut(s) 215
Eam1104I CTCTTC 1 cut(s) 722
EarI CTCTTC 1 cut(s) 722
Eco57I CTGAAG 2 cut(s) 153, 660
EcoO109I RGGNCCY 1 cut(s) 310
FaeI CATG 2 cut(s) 580, 697
FaqI GGGAC 1 cut(s) 526
FatI CATG 2 cut(s) 576, 693
FauI CCCGC 1 cut(s) 262
FauNDI CATATG 1 cut(s) 517
FbaI TGATCA 1 cut(s) 379
Fnu4HI GCNGC 2 cut(s) 38, 155
Fsp4HI GCNGC 2 cut(s) 38, 155
GluI GCNGC 2 cut(s) 38, 155
HaeIII GGCC 2 cut(s) 217, 312
Hin1II CATG 2 cut(s) 580, 697
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HinfI GANTC 1 cut(s) 724
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 3 cut(s) 19, 26, 485
Hpy188I TCNGA 2 cut(s) 331, 679
Hpy188III TCNNGA 3 cut(s) 453, 501, 721
Hpy8I GTNNAC 3 cut(s) 19, 26, 485
HpyAV CCTTC 2 cut(s) 301, 369
HpyCH4III ACNGT 3 cut(s) 23, 540, 572
HpyCH4V TGCA 9 cut(s) 26, 37, 122, 322, 348, 396, 437, 485, 712
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 434, 635
HpyF3I CTNAG 2 cut(s) 264, 609
Hsp92II CATG 2 cut(s) 580, 697
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 3 cut(s) 58, 379, 690
LmnI GCTCC 1 cut(s) 626
LpnPI CCDG 8 cut(s) 141, 207, 300, 449, 466, 478, 667, 734
Lsp1109I GCAGC 2 cut(s) 49, 166
MaeIII GTNAC 1 cut(s) 70
MalI GATC 3 cut(s) 60, 381, 692
MboI GATC 3 cut(s) 58, 379, 690
MboII GAAGA 6 cut(s) 17, 97, 146, 533, 666, 709
MhlI GDGCHC 2 cut(s) 28, 487
MlsI TGGCCA 1 cut(s) 217
MluCI AATT 3 cut(s) 53, 101, 333
MluNI TGGCCA 1 cut(s) 217
MmeI TCCRAC 2 cut(s) 226, 663
MnlI CCTC 5 cut(s) 323, 331, 409, 418, 675
Mox20I TGGCCA 1 cut(s) 217
MscI TGGCCA 1 cut(s) 217
MseI TTAA 4 cut(s) 45, 150, 173, 443
MslI CAYNNNNRTG 1 cut(s) 211
Msp20I TGGCCA 1 cut(s) 217
MspA1I CMGCKG 1 cut(s) 131
MwoI GCNNNNNNNGC 3 cut(s) 128, 434, 635
NdeI CATATG 1 cut(s) 517
NdeII GATC 3 cut(s) 58, 379, 690
NlaIII CATG 2 cut(s) 580, 697
NmuCI GTSAC 1 cut(s) 70
PaqCI CACCTGC 1 cut(s) 2
PfeI GAWTC 1 cut(s) 724
PkrI GCNGC 2 cut(s) 39, 156
PshBI ATTAAT 1 cut(s) 45
PsiI TTATAA 1 cut(s) 621
PspPI GGNCC 1 cut(s) 310
PsrI GAACNNNNNNTAC 2 cut(s) 267, 299
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 1 cut(s) 285
RsaNI GTAC 1 cut(s) 284
RseI CAYNNNNRTG 1 cut(s) 211
SaqAI TTAA 4 cut(s) 45, 150, 173, 443
SatI GCNGC 2 cut(s) 38, 155
Sau3AI GATC 3 cut(s) 58, 379, 690
Sau96I GGNCC 1 cut(s) 310
SduI GDGCHC 2 cut(s) 28, 487
SetI ASST 6 cut(s) 16, 133, 159, 450, 468, 667
SmiMI CAYNNNNRTG 1 cut(s) 211
Sse9I AATT 3 cut(s) 53, 101, 333
SsiI CCGC 1 cut(s) 269
TaaI ACNGT 3 cut(s) 23, 540, 572
TaqI TCGA 1 cut(s) 57
TaqII GACCGA 1 cut(s) 305
TasI AATT 3 cut(s) 53, 101, 333
TfiI GAWTC 1 cut(s) 724
Tru1I TTAA 4 cut(s) 45, 150, 173, 443
Tru9I TTAA 4 cut(s) 45, 150, 173, 443
TscAI CASTG 3 cut(s) 543, 592, 734
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 2 cut(s) 37, 154
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 3 cut(s) 255, 320, 534
TspRI CASTG 3 cut(s) 543, 592, 734
VneI GTGCAC 2 cut(s) 24, 483
VspI ATTAAT 1 cut(s) 45
XapI RAATTY 2 cut(s) 53, 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.