Rroxscaffold_5G00371330

Amidohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015263
Physical Location & Seq
Reverse (-)
52924824 .. 52928122
3299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371330.1

Sequence Viewer

Length: 999 bp
ATGGCAATGGCAGTGAGGCTAACACCTGTTCTGGTTTTAGCTGAAACCAAGTTTAAACACTTTGCCTTCAGTTCAATAAAAAACCAGTTCAGGGTCTCTGCTGCAGCAATGGCTACAGGTGGAACACAGAGCAGAACTAAAGTCATAGATTCACATCTCCATGTGTGGGCTTCACCTCAAGAGGCTGCTGATAAATATCCTTACTTTCCTGGGCAAGAACCCACTTTGCCTGGAGATGTCGATTTATTGCTCAAGTGTATGGAAGAAGCAGGTGTTGACGGTGCACTTATCGTGCAGCCTATTAATCACAAATTCGATCACTCTTTGGTGACAGGTGTATTGAAGAAGTATCCATCTAAATTTGTTGGTTGTTGCCTTGCAAATCCAGCTGAAGATGGGAGTGGGGTTAACCAGCTTGAACATCTTATTTTAAAGGATAACTATCGTGCTGTTCGCTTCAATCCATATTTATGGCCTTCTGGTCAAAAGATGACCAATGAAGTTGGAAAGGCAATGTTCTCTAAGGCGGGAGAACTTGGAGTACCAGTTGGCTTTATGTGTATGAAGGGCCTCGGTCTGCATATTTCAGAAATTGAGGAACTATGCACAGAGTTTCCTTCAACACTTGTATTACTTGATCATTTGGCTTTCTGCAAACCACCAATAAATGATGAGGAGAATGAGGCTTTCTCTGCACTTTTAAAGCTATCCAGATTTCCACAGGTCTATGTAAAAGTAAGTGCACTTTTCAGGGTGTCAAGAACGCCATTTCCATATGAAGATGTATCTCACACACTGTCCCAAGTTGTGTCAAGTTTTGGTGCTAACCGTGTCATGTGGGGCAGTGATTTTCCATTTGTTGTTGCTGAGTGTGGTTATAAACGAGCAAAAGAAGCCGTTTCTTTGATTGCCAGTAAAATACCTCTATCATCTTCAGAGTTGGAGTGGATCATGGGTAGAACAATCTTGCAACTCTTCCAGAATCAGTGGCTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_2

Protein Analysis

332

Amino Acids

36.78

Weight (kDa)

6.76

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_2 PF04909 49 - 326 8.5e-30 Amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35450
fragaria_vesca FvH4_4g23290 FvH4_4g23290
malus_domestica MD16G1123900.v1.1
prunus_persica Prupe.1G221700_v2.0.a1
pyrus_communis pycom16g10590
rosa_chinensis RchiOBHm_Chr4g0429651
rosa_laevigata RLG00000007055
rosa_multiflora Rmu_sc0006590.1_g000006
rosa_roxburghii Rroxscaffold_5G00371330
rosa_rugosa Rorug04G0237500
rosa_samantha Rh4AG293500 Rh4BG299300 Rh4CG315100 Rh4DG296400
rosa_wichuraiana Rw4G025400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 879
AarI CACCTGC 1 cut(s) 260
Acc36I ACCTGC 1 cut(s) 260
AciI CCGC 1 cut(s) 527
AclWI GGATC 1 cut(s) 956
AcsI RAATTY 2 cut(s) 311, 359
AcuI CTGAAG 3 cut(s) 52, 411, 918
AfaI GTAC 1 cut(s) 543
AfiI CCNNNNNNNGG 2 cut(s) 91, 166
AgsI TTSAA 5 cut(s) 75, 343, 419, 460, 621
AjnI CCWGG 2 cut(s) 208, 229
AloI GAACNNNNNNTCC 2 cut(s) 754, 786
AluBI AGCT 4 cut(s) 41, 389, 415, 706
AluI AGCT 4 cut(s) 41, 389, 415, 706
Alw21I GWGCWC 2 cut(s) 286, 745
Alw26I GTCTC 1 cut(s) 100
Alw44I GTGCAC 2 cut(s) 282, 741
AlwI GGATC 1 cut(s) 956
AoxI GGCC 2 cut(s) 473, 568
ApaLI GTGCAC 2 cut(s) 282, 741
ApeKI GCWGC 4 cut(s) 101, 104, 185, 295
ApoI RAATTY 2 cut(s) 311, 359
AseI ATTAAT 1 cut(s) 303
AspS9I GGNCC 1 cut(s) 568
AsuHPI GGTGA 2 cut(s) 165, 340
BaeGI GKGCMC 2 cut(s) 286, 745
Bbv12I GWGCWC 2 cut(s) 286, 745
BbvI GCAGC 4 cut(s) 88, 116, 172, 307
BccI CCATC 2 cut(s) 361, 389
BceAI ACGGC 1 cut(s) 881
BciT130I CCWGG 2 cut(s) 210, 231
BciVI GTATCC 1 cut(s) 360
BclI TGATCA 1 cut(s) 637
BcoDI GTCTC 1 cut(s) 100
BfmI CTRYAG 2 cut(s) 102, 114
BfuAI ACCTGC 1 cut(s) 260
BfuI GTATCC 1 cut(s) 360
BisI GCNGC 4 cut(s) 102, 105, 186, 296
BlsI GCNGC 4 cut(s) 103, 106, 187, 297
Bme1390I CCNGG 2 cut(s) 210, 231
BmgT120I GGNCC 1 cut(s) 568
BmrFI CCNGG 2 cut(s) 210, 231
BplI GAGNNNNNCTC 2 cut(s) 674, 706
BpmI CTGGAG 1 cut(s) 252
BpuEI CTTGAG 2 cut(s) 162, 236
BsaBI GATNNNNATC 3 cut(s) 153, 195, 441
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 2 cut(s) 209, 571
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 166
Bse1I ACTGG 3 cut(s) 85, 545, 912
Bse3DI GCAATG 3 cut(s) 12, 114, 519
Bse8I GATNNNNATC 3 cut(s) 153, 195, 441
BseBI CCWGG 2 cut(s) 210, 231
BseDI CCNNGG 2 cut(s) 209, 571
BseJI GATNNNNATC 3 cut(s) 153, 195, 441
BseLI CCNNNNNNNGG 2 cut(s) 91, 166
BseMI GCAATG 3 cut(s) 12, 114, 519
BseMII CTCAG 1 cut(s) 858
BseNI ACTGG 3 cut(s) 85, 545, 912
BseRI GAGGAG 1 cut(s) 689
BseSI GKGCMC 2 cut(s) 286, 745
BseXI GCAGC 4 cut(s) 88, 116, 172, 307
BsgI GTGCAG 2 cut(s) 314, 678
BshFI GGCC 2 cut(s) 475, 570
BsiHKAI GWGCWC 2 cut(s) 286, 745
BslFI GGGAC 1 cut(s) 784
BslI CCNNNNNNNGG 2 cut(s) 91, 166
BsmAI GTCTC 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 784
BsnI GGCC 2 cut(s) 475, 570
Bso31I GGTCTC 1 cut(s) 100
Bsp1286I GDGCHC 2 cut(s) 286, 745
Bsp143I GATC 3 cut(s) 316, 637, 948
BspACI CCGC 1 cut(s) 527
BspANI GGCC 2 cut(s) 475, 570
BspCNI CTCAG 1 cut(s) 859
BspMAI CTGCAG 1 cut(s) 106
BspMI ACCTGC 1 cut(s) 260
BspPI GGATC 1 cut(s) 956
BspTNI GGTCTC 1 cut(s) 100
BsrDI GCAATG 3 cut(s) 12, 114, 519
BsrI ACTGG 3 cut(s) 85, 545, 912
BssECI CCNNGG 2 cut(s) 209, 571
BssMI GATC 3 cut(s) 316, 637, 948
Bst2UI CCWGG 2 cut(s) 210, 231
Bst4CI ACNGT 3 cut(s) 281, 798, 830
Bst6I CTCTTC 1 cut(s) 980
BstDEI CTNAG 2 cut(s) 522, 867
BstKTI GATC 3 cut(s) 319, 640, 951
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 3 cut(s) 316, 637, 948
BstMWI GCNNNNNNNGC 4 cut(s) 110, 386, 692, 893
BstNI CCWGG 2 cut(s) 210, 231
BstSCI CCNGG 2 cut(s) 208, 229
BstSFI CTRYAG 2 cut(s) 102, 114
BstSLI GKGCMC 2 cut(s) 286, 745
BstV1I GCAGC 4 cut(s) 88, 116, 172, 307
BstXI CCANNNNNNTGG 1 cut(s) 471
BsuI GTATCC 1 cut(s) 360
BsuRI GGCC 2 cut(s) 475, 570
BtsI GCAGTG 2 cut(s) 18, 850
BtsIMutI CAGTG 4 cut(s) 18, 794, 850, 992
BveI ACCTGC 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 568
Csp6I GTAC 1 cut(s) 542
CviAII CATG 3 cut(s) 161, 835, 952
CviQI GTAC 1 cut(s) 542
DdeI CTNAG 2 cut(s) 522, 867
DpnI GATC 3 cut(s) 318, 639, 950
DpnII GATC 3 cut(s) 316, 637, 948
DraI TTTAAA 3 cut(s) 55, 432, 702
Eam1104I CTCTTC 1 cut(s) 980
EarI CTCTTC 1 cut(s) 980
Eco31I GGTCTC 1 cut(s) 100
Eco57I CTGAAG 3 cut(s) 52, 411, 918
EcoO109I RGGNCCY 1 cut(s) 568
EcoRII CCWGG 2 cut(s) 208, 229
FaeI CATG 3 cut(s) 164, 838, 955
FaqI GGGAC 1 cut(s) 784
FatI CATG 3 cut(s) 160, 834, 951
FauI CCCGC 1 cut(s) 520
FauNDI CATATG 1 cut(s) 775
FbaI TGATCA 1 cut(s) 637
Fnu4HI GCNGC 4 cut(s) 102, 105, 186, 296
Fsp4HI GCNGC 4 cut(s) 102, 105, 186, 296
GluI GCNGC 4 cut(s) 102, 105, 186, 296
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 2 cut(s) 475, 570
Hin1II CATG 3 cut(s) 164, 838, 955
HincII GTYRAC 2 cut(s) 277, 409
HindII GTYRAC 2 cut(s) 277, 409
HinfI GANTC 2 cut(s) 149, 982
HpaI GTTAAC 1 cut(s) 409
HphI GGTGA 2 cut(s) 165, 340
Hpy166II GTNNAC 4 cut(s) 277, 284, 409, 743
Hpy188I TCNGA 2 cut(s) 589, 937
Hpy188III TCNNGA 4 cut(s) 179, 711, 759, 979
Hpy8I GTNNAC 4 cut(s) 277, 284, 409, 743
HpyAV CCTTC 4 cut(s) 76, 486, 559, 627
HpyCH4III ACNGT 3 cut(s) 281, 798, 830
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 386, 692, 893
HpyF3I CTNAG 2 cut(s) 522, 867
Hsp92II CATG 3 cut(s) 164, 838, 955
Ksp22I TGATCA 1 cut(s) 637
KspAI GTTAAC 1 cut(s) 409
Kzo9I GATC 3 cut(s) 316, 637, 948
Lsp1109I GCAGC 4 cut(s) 88, 116, 172, 307
MaeIII GTNAC 1 cut(s) 328
MalI GATC 3 cut(s) 318, 639, 950
MboI GATC 3 cut(s) 316, 637, 948
MboII GAAGA 6 cut(s) 275, 355, 404, 791, 924, 967
MhlI GDGCHC 2 cut(s) 286, 745
MluCI AATT 3 cut(s) 311, 359, 591
MmeI TCCRAC 2 cut(s) 484, 921
MnlI CCTC 8 cut(s) 9, 175, 186, 581, 589, 667, 676, 933
MseI TTAA 5 cut(s) 54, 303, 408, 431, 701
MslI CAYNNNNRTG 2 cut(s) 159, 469
MspA1I CMGCKG 1 cut(s) 389
MspR9I CCNGG 2 cut(s) 210, 231
MssI GTTTAAAC 1 cut(s) 55
MvaI CCWGG 2 cut(s) 210, 231
MwoI GCNNNNNNNGC 4 cut(s) 110, 386, 692, 893
NdeI CATATG 1 cut(s) 775
NdeII GATC 3 cut(s) 316, 637, 948
NlaIII CATG 3 cut(s) 164, 838, 955
NmuCI GTSAC 1 cut(s) 328
PaqCI CACCTGC 1 cut(s) 260
PfeI GAWTC 2 cut(s) 149, 982
PkrI GCNGC 4 cut(s) 103, 106, 187, 297
PmeI GTTTAAAC 1 cut(s) 55
PshBI ATTAAT 1 cut(s) 303
PsiI TTATAA 1 cut(s) 879
Psp6I CCWGG 2 cut(s) 208, 229
PspGI CCWGG 2 cut(s) 208, 229
PspPI GGNCC 1 cut(s) 568
PsrI GAACNNNNNNTAC 2 cut(s) 525, 557
PstI CTGCAG 1 cut(s) 106
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 1 cut(s) 543
RsaNI GTAC 1 cut(s) 542
RseI CAYNNNNRTG 2 cut(s) 159, 469
SaqAI TTAA 5 cut(s) 54, 303, 408, 431, 701
SatI GCNGC 4 cut(s) 102, 105, 186, 296
Sau3AI GATC 3 cut(s) 316, 637, 948
Sau96I GGNCC 1 cut(s) 568
ScrFI CCNGG 2 cut(s) 210, 231
SduI GDGCHC 2 cut(s) 286, 745
SfcI CTRYAG 2 cut(s) 102, 114
SmiMI CAYNNNNRTG 2 cut(s) 159, 469
SmlI CTYRAG 2 cut(s) 177, 251
SmoI CTYRAG 2 cut(s) 177, 251
Sse9I AATT 3 cut(s) 311, 359, 591
SsiI CCGC 1 cut(s) 527
StyD4I CCNGG 2 cut(s) 208, 229
TaaI ACNGT 3 cut(s) 281, 798, 830
TaqI TCGA 2 cut(s) 240, 315
TaqII GACCGA 1 cut(s) 563
TasI AATT 3 cut(s) 311, 359, 591
TfiI GAWTC 2 cut(s) 149, 982
Tru1I TTAA 5 cut(s) 54, 303, 408, 431, 701
Tru9I TTAA 5 cut(s) 54, 303, 408, 431, 701
TscAI CASTG 4 cut(s) 18, 801, 850, 992
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 4 cut(s) 101, 104, 185, 295
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 3 cut(s) 513, 578, 792
TspRI CASTG 4 cut(s) 18, 801, 850, 992
VneI GTGCAC 2 cut(s) 282, 741
VspI ATTAAT 1 cut(s) 303
XapI RAATTY 2 cut(s) 311, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.