Rh4DG296400

Amidohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0015263
Physical Location & Seq
Reverse (-)
51838050 .. 51841868
3819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG296400.1

Sequence Viewer

Length: 735 bp
ATGGCAATGGCAGTGAGGCTAACACCTGTTCTGGCTCAGTTTAAACACTTTGCCTTCAGTTCAAGAGAAAACCAGTTCAGGGTCTCGGCTGCAGCAATGGCTACAGGTGGACCACAGAGCAGAACTAAAGTCATAGATTCACATCTCCATGTGTGGGCCTCACCTCAAGAGGATAACTATCGTGCTGTTCGCTTCAATCCATATTTATGGCCTTCTGGTCAAAAGATGACCAATGAAGTTGGAAAGGCAATGTTCTCCAAGGCGGGAGAACTTGGAGTACCAGTTGGCTTTATGTGTATGAAGGGCCTCGGTCTGCATATTTCAGAAATTGAGGAACTATGCACAGAGTTTCCTTCAACACTTGTATTACTTGATCATTTGGCTTTCTGCAAACCACCAATAAATGATGAGGAAAATGAGGCTTTCTCTGCACTTTTAAAGCTATCCAGATTTCCACAGGTCTATGTAAAAGTAAGTGCACTTTTCAGGGTGTCAAGAATGGCATTTCCATATGAAGATGTATCTCACACACTGTCCCAAGTTGTGTCAAGTTTTGGTGCTAACCGGGTCATGTGGGGCAGTGATTTTCCATTTGTTGTTGCTGAGTGTGGTTATAAAGGAGCAAAAGAAGCCGTTTCTTTGATTGCCAGTAAAATACCTCTATCATCTTCAGAGTTGGAGTGGATCATGGGTAGAACAATCTTGCAACTCTTCCAGAATCAGTGGCTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_2

Protein Analysis

244

Amino Acids

27.23

Weight (kDa)

7.71

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_2 PF04909 57 - 238 1.4e-21 Amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35450
fragaria_vesca FvH4_4g23290 FvH4_4g23290
malus_domestica MD16G1123900.v1.1
prunus_persica Prupe.1G221700_v2.0.a1
pyrus_communis pycom16g10590
rosa_chinensis RchiOBHm_Chr4g0429651
rosa_laevigata RLG00000007055
rosa_multiflora Rmu_sc0006590.1_g000006
rosa_roxburghii Rroxscaffold_5G00371330
rosa_rugosa Rorug04G0237500
rosa_samantha Rh4AG293500 Rh4BG299300 Rh4CG315100 Rh4DG296400
rosa_wichuraiana Rw4G025400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 615
AciI CCGC 1 cut(s) 263
AclWI GGATC 1 cut(s) 692
AcuI CTGAAG 2 cut(s) 40, 654
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 2 cut(s) 79, 154
AgsI TTSAA 3 cut(s) 63, 196, 357
AluBI AGCT 1 cut(s) 442
AluI AGCT 1 cut(s) 442
Alw21I GWGCWC 1 cut(s) 481
Alw26I GTCTC 1 cut(s) 88
Alw44I GTGCAC 1 cut(s) 477
AlwI GGATC 1 cut(s) 692
AoxI GGCC 3 cut(s) 156, 209, 304
ApaLI GTGCAC 1 cut(s) 477
ApeKI GCWGC 2 cut(s) 89, 92
AspS9I GGNCC 3 cut(s) 110, 156, 304
AsuC2I CCSGG 1 cut(s) 566
AsuHPI GGTGA 1 cut(s) 153
AvaII GGWCC 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 481
Bbv12I GWGCWC 1 cut(s) 481
BbvI GCAGC 2 cut(s) 76, 104
BceAI ACGGC 1 cut(s) 617
BclI TGATCA 1 cut(s) 373
BcnI CCSGG 1 cut(s) 566
BcoDI GTCTC 1 cut(s) 88
BfmI CTRYAG 2 cut(s) 90, 102
BisI GCNGC 2 cut(s) 90, 93
BlsI GCNGC 2 cut(s) 91, 94
Bme1390I CCNGG 1 cut(s) 566
Bme18I GGWCC 1 cut(s) 110
BmgT120I GGNCC 3 cut(s) 110, 156, 304
BmrFI CCNGG 1 cut(s) 566
BplI GAGNNNNNCTC 2 cut(s) 410, 442
BpuEI CTTGAG 1 cut(s) 150
BpuMI CCSGG 1 cut(s) 566
BsaBI GATNNNNATC 2 cut(s) 141, 177
BsaI GGTCTC 1 cut(s) 88
BsaJI CCNNGG 2 cut(s) 258, 307
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 154
Bse1I ACTGG 3 cut(s) 73, 281, 648
Bse3DI GCAATG 3 cut(s) 12, 102, 255
Bse8I GATNNNNATC 2 cut(s) 141, 177
BseDI CCNNGG 2 cut(s) 258, 307
BseJI GATNNNNATC 2 cut(s) 141, 177
BseLI CCNNNNNNNGG 2 cut(s) 79, 154
BseMI GCAATG 3 cut(s) 12, 102, 255
BseMII CTCAG 2 cut(s) 50, 594
BseNI ACTGG 3 cut(s) 73, 281, 648
BseSI GKGCMC 1 cut(s) 481
BseXI GCAGC 2 cut(s) 76, 104
BsgI GTGCAG 1 cut(s) 414
BshFI GGCC 3 cut(s) 158, 211, 306
BsiHKAI GWGCWC 1 cut(s) 481
BsiSI CCGG 1 cut(s) 565
BslFI GGGAC 1 cut(s) 520
BslI CCNNNNNNNGG 2 cut(s) 79, 154
BsmAI GTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 520
BsnI GGCC 3 cut(s) 158, 211, 306
Bso31I GGTCTC 1 cut(s) 88
Bsp1286I GDGCHC 1 cut(s) 481
Bsp143I GATC 2 cut(s) 373, 684
BspACI CCGC 1 cut(s) 263
BspANI GGCC 3 cut(s) 158, 211, 306
BspCNI CTCAG 2 cut(s) 49, 595
BspMAI CTGCAG 1 cut(s) 94
BspPI GGATC 1 cut(s) 692
BspTNI GGTCTC 1 cut(s) 88
BsrDI GCAATG 3 cut(s) 12, 102, 255
BsrI ACTGG 3 cut(s) 73, 281, 648
BssECI CCNNGG 2 cut(s) 258, 307
BssMI GATC 2 cut(s) 373, 684
BssT1I CCWWGG 1 cut(s) 258
Bst4CI ACNGT 1 cut(s) 534
Bst6I CTCTTC 1 cut(s) 716
BstDEI CTNAG 2 cut(s) 36, 603
BstKTI GATC 2 cut(s) 376, 687
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 2 cut(s) 373, 684
BstMWI GCNNNNNNNGC 3 cut(s) 98, 428, 629
BstSCI CCNGG 1 cut(s) 564
BstSFI CTRYAG 2 cut(s) 90, 102
BstSLI GKGCMC 1 cut(s) 481
BstV1I GCAGC 2 cut(s) 76, 104
BstXI CCANNNNNNTGG 1 cut(s) 207
BsuRI GGCC 3 cut(s) 158, 211, 306
BtsI GCAGTG 2 cut(s) 18, 586
BtsIMutI CAGTG 4 cut(s) 18, 530, 586, 728
Cfr13I GGNCC 3 cut(s) 110, 156, 304
Csp6I GTAC 1 cut(s) 278
CviAII CATG 3 cut(s) 149, 571, 688
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 2 cut(s) 36, 603
DpnI GATC 2 cut(s) 375, 686
DpnII GATC 2 cut(s) 373, 684
DraI TTTAAA 2 cut(s) 43, 438
Eam1104I CTCTTC 1 cut(s) 716
EarI CTCTTC 1 cut(s) 716
Eco130I CCWWGG 1 cut(s) 258
Eco31I GGTCTC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 110
Eco57I CTGAAG 2 cut(s) 40, 654
EcoO109I RGGNCCY 1 cut(s) 304
EcoT14I CCWWGG 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 3 cut(s) 152, 574, 691
FaqI GGGAC 1 cut(s) 520
FatI CATG 3 cut(s) 148, 570, 687
FauI CCCGC 1 cut(s) 256
FauNDI CATATG 1 cut(s) 511
FbaI TGATCA 1 cut(s) 373
Fnu4HI GCNGC 2 cut(s) 90, 93
Fsp4HI GCNGC 2 cut(s) 90, 93
GluI GCNGC 2 cut(s) 90, 93
HaeIII GGCC 3 cut(s) 158, 211, 306
HapII CCGG 1 cut(s) 565
Hin1II CATG 3 cut(s) 152, 574, 691
HinfI GANTC 2 cut(s) 137, 718
HpaII CCGG 1 cut(s) 565
HphI GGTGA 1 cut(s) 153
Hpy166II GTNNAC 2 cut(s) 110, 479
Hpy188I TCNGA 2 cut(s) 325, 673
Hpy188III TCNNGA 5 cut(s) 63, 167, 447, 495, 715
Hpy8I GTNNAC 2 cut(s) 110, 479
HpyAV CCTTC 4 cut(s) 64, 222, 295, 363
HpyCH4III ACNGT 1 cut(s) 534
HpyCH4V TGCA 7 cut(s) 92, 316, 342, 390, 431, 479, 706
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 428, 629
HpyF3I CTNAG 2 cut(s) 36, 603
Hsp92II CATG 3 cut(s) 152, 574, 691
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 2 cut(s) 373, 684
LmnI GCTCC 1 cut(s) 620
Lsp1109I GCAGC 2 cut(s) 76, 104
MalI GATC 2 cut(s) 375, 686
MboI GATC 2 cut(s) 373, 684
MboII GAAGA 3 cut(s) 527, 660, 703
MhlI GDGCHC 1 cut(s) 481
MluCI AATT 1 cut(s) 327
MmeI TCCRAC 2 cut(s) 220, 657
MnlI CCTC 9 cut(s) 9, 163, 169, 174, 317, 325, 403, 412, 669
MseI TTAA 2 cut(s) 42, 437
MslI CAYNNNNRTG 2 cut(s) 147, 205
MspI CCGG 1 cut(s) 565
MspR9I CCNGG 1 cut(s) 566
MssI GTTTAAAC 1 cut(s) 43
MwoI GCNNNNNNNGC 3 cut(s) 98, 428, 629
NciI CCSGG 1 cut(s) 566
NdeI CATATG 1 cut(s) 511
NdeII GATC 2 cut(s) 373, 684
NlaIII CATG 3 cut(s) 152, 574, 691
NmeAIII GCCGAG 1 cut(s) 65
PfeI GAWTC 2 cut(s) 137, 718
PkrI GCNGC 2 cut(s) 91, 94
PmeI GTTTAAAC 1 cut(s) 43
PsiI TTATAA 1 cut(s) 615
PspPI GGNCC 3 cut(s) 110, 156, 304
PsrI GAACNNNNNNTAC 2 cut(s) 261, 293
PstI CTGCAG 1 cut(s) 94
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
RseI CAYNNNNRTG 2 cut(s) 147, 205
SaqAI TTAA 2 cut(s) 42, 437
SatI GCNGC 2 cut(s) 90, 93
Sau3AI GATC 2 cut(s) 373, 684
Sau96I GGNCC 3 cut(s) 110, 156, 304
ScrFI CCNGG 1 cut(s) 566
SduI GDGCHC 1 cut(s) 481
SetI ASST 6 cut(s) 28, 109, 166, 444, 462, 661
SfcI CTRYAG 2 cut(s) 90, 102
SinI GGWCC 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 147, 205
SmlI CTYRAG 1 cut(s) 165
SmoI CTYRAG 1 cut(s) 165
Sse9I AATT 1 cut(s) 327
SsiI CCGC 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 564
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 1 cut(s) 534
TaqII GACCGA 1 cut(s) 299
TasI AATT 1 cut(s) 327
TfiI GAWTC 2 cut(s) 137, 718
Tru1I TTAA 2 cut(s) 42, 437
Tru9I TTAA 2 cut(s) 42, 437
TscAI CASTG 4 cut(s) 18, 537, 586, 728
TseI GCWGC 2 cut(s) 89, 92
TspDTI ATGAA 3 cut(s) 249, 314, 528
TspRI CASTG 4 cut(s) 18, 537, 586, 728
VneI GTGCAC 1 cut(s) 477
VpaK11BI GGWCC 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.