MD16G1123900.v1.1

Amidohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015263
Physical Location & Seq
Forward (+)
8950174 .. 8952879
2706 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1123900.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGGCAACGGCCGCGAGGGTCACATCTCTTCTGCTTTCACCTCCAGCTGCACTCCCTTTGGTACCACTTAAACGTACGCGACCATTTGCCCACATTTCAACGAAGACCCGGCTCATTGCCTCTGCTTCTGCTGCTGCTGCTTCAATGGCTTCCACTGCCAGTAAACCCAAAATCATAGATTCACATCTGCATGTGTGGGCATCCCCTCAAGAGGCTGCGGATAAATACCCTTACTTTCCTGGCCAAGAACCCACTTTGCCTGGAGATGTCGATTTCTTGCTTCAGTGTATGGAAGAAGCAGGTGTTGACGGTGCACTTATCGTGCAGCCCATTAATCACAAATTTGATCATTCTTTGGTGACAAGTGTGTTGAAGAAGTACCCTTCTAAATTTGTCGGTTGTTGTCTAGCAAATCCAGCCGAAGATGGAAGTGGGATTAAGCAGCTTGAAAATCTCATTTTGAAGGATAACTATCGTGCTGTTCGCTTCAATCCATATTTGTGGCCTTCTGGTCAACAGATGACCAATGAAGTAGGAAAGGCAATGTTCTCTAAGGCAGGAGAACTTGGAGTACCAGTTGGTTTCATGTGTATGAAGGGCCTCGGTTTGCACATATCAGAAATTGAGGAGCTGTGCACGGAGTTTCCATCAACAGCTGTATTGCTTGATCATTTGGCTTTCTGCAAACCACCAATAAACGATGAGGAAAAGCTCGCTTTCTCTGCACTTTTACAGCTATCCAGATTCCCACAGGTCTATGTCAAATTCAGTGCGCTGTTCCGGTTGTCGAGAATGCCATTTCCGTACGAGGATTTATCTCATTTACTGTCCCAAGTCGTGTCGAGCTTTGGTGCTAACCGCGTCATGTGGGGCAGTGATTTTCCATTTGTTGTTCCCGAGTGTGGCTATAAAGGAGCCAAAGAAGCCGTTTCTCTCATCGTCAATCGAGTGCCGCTATCGTCTTCGGAGTTGGAGTCGATCATGGGAAGAACAGCGTTGCAACTCTTCCGAAATCAATGGCTTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_2

Protein Analysis

343

Amino Acids

37.51

Weight (kDa)

7.62

Isoelectric Point (pI)

48.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_2 PF04909 59 - 337 1.9e-31 Amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35450
fragaria_vesca FvH4_4g23290 FvH4_4g23290
malus_domestica MD16G1123900.v1.1
prunus_persica Prupe.1G221700_v2.0.a1
pyrus_communis pycom16g10590
rosa_chinensis RchiOBHm_Chr4g0429651
rosa_laevigata RLG00000007055
rosa_multiflora Rmu_sc0006590.1_g000006
rosa_roxburghii Rroxscaffold_5G00371330
rosa_rugosa Rorug04G0237500
rosa_samantha Rh4AG293500 Rh4BG299300 Rh4CG315100 Rh4DG296400
rosa_wichuraiana Rw4G025400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 290
Acc36I ACCTGC 1 cut(s) 290
Acc65I GGTACC 1 cut(s) 61
AccB1I GGYRCC 1 cut(s) 61
AccII CGCG 3 cut(s) 14, 79, 861
AciI CCGC 4 cut(s) 12, 218, 859, 953
AcoI YGGCCR 2 cut(s) 9, 241
AcsI RAATTY 3 cut(s) 341, 389, 764
AcuI CTGAAG 1 cut(s) 266
AfaI GTAC 5 cut(s) 63, 76, 380, 573, 806
AfiI CCNNNNNNNGG 2 cut(s) 211, 902
AgsI TTSAA 6 cut(s) 99, 144, 373, 449, 463, 490
AjnI CCWGG 2 cut(s) 238, 259
AluBI AGCT 7 cut(s) 47, 445, 631, 656, 712, 736, 846
AluI AGCT 7 cut(s) 47, 445, 631, 656, 712, 736, 846
Alw21I GWGCWC 2 cut(s) 316, 638
Alw44I GTGCAC 2 cut(s) 312, 634
Ama87I CYCGRG 1 cut(s) 896
AoxI GGCC 4 cut(s) 9, 241, 503, 598
ApaLI GTGCAC 2 cut(s) 312, 634
ApeKI GCWGC 7 cut(s) 47, 131, 134, 137, 215, 325, 442
ApoI RAATTY 3 cut(s) 341, 389, 764
AseI ATTAAT 1 cut(s) 333
Asp718I GGTACC 1 cut(s) 61
AspLEI GCGC 1 cut(s) 775
AspS9I GGNCC 1 cut(s) 598
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 2 cut(s) 30, 370
AvaI CYCGRG 1 cut(s) 896
BaeGI GKGCMC 2 cut(s) 316, 638
BalI TGGCCA 1 cut(s) 243
BanI GGYRCC 1 cut(s) 61
BbsI GAAGAC 2 cut(s) 110, 954
Bbv12I GWGCWC 2 cut(s) 316, 638
BbvI GCAGC 7 cut(s) 34, 118, 121, 124, 202, 337, 454
BccI CCATC 2 cut(s) 419, 655
BceAI ACGGC 2 cut(s) 24, 911
BciT130I CCWGG 2 cut(s) 240, 261
BclI TGATCA 2 cut(s) 346, 667
BcnI CCSGG 1 cut(s) 109
BfaI CTAG 1 cut(s) 407
BfuAI ACCTGC 1 cut(s) 290
BisI GCNGC 9 cut(s) 12, 48, 132, 135, 138, 216, 326, 443, 953
BlsI GCNGC 9 cut(s) 13, 49, 133, 136, 139, 217, 327, 444, 954
Bme1390I CCNGG 3 cut(s) 109, 240, 261
BmeT110I CYCGRG 1 cut(s) 896
BmgT120I GGNCC 1 cut(s) 598
BmiI GGNNCC 2 cut(s) 63, 916
BmrFI CCNGG 3 cut(s) 109, 240, 261
BmsI GCATC 1 cut(s) 209
BpiI GAAGAC 2 cut(s) 110, 954
BpmI CTGGAG 2 cut(s) 27, 282
BpuEI CTTGAG 1 cut(s) 192
BpuMI CCSGG 1 cut(s) 109
BsaBI GATNNNNATC 2 cut(s) 183, 471
BsaJI CCNNGG 1 cut(s) 601
BsaWI WCCGGW 1 cut(s) 780
BsaXI ACNNNNNCTCC 4 cut(s) 561, 591, 959, 989
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 902
Bse1I ACTGG 2 cut(s) 159, 575
Bse3DI GCAATG 2 cut(s) 114, 549
Bse8I GATNNNNATC 2 cut(s) 183, 471
BseBI CCWGG 2 cut(s) 240, 261
BseDI CCNNGG 1 cut(s) 601
BseGI GGATG 1 cut(s) 200
BseJI GATNNNNATC 2 cut(s) 183, 471
BseLI CCNNNNNNNGG 2 cut(s) 211, 902
BseMI GCAATG 2 cut(s) 114, 549
BseNI ACTGG 2 cut(s) 159, 575
BseRI GAGGAG 1 cut(s) 641
BseSI GKGCMC 2 cut(s) 316, 638
BseX3I CGGCCG 1 cut(s) 9
BseXI GCAGC 7 cut(s) 34, 118, 121, 124, 202, 337, 454
BsgI GTGCAG 3 cut(s) 33, 344, 708
Bsh1236I CGCG 3 cut(s) 14, 79, 861
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 4 cut(s) 11, 243, 505, 600
BshNI GGYRCC 1 cut(s) 61
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 316, 638
BsiHKCI CYCGRG 1 cut(s) 896
BsiSI CCGG 2 cut(s) 109, 781
BsiWI CGTACG 2 cut(s) 74, 804
BslFI GGGAC 1 cut(s) 814
BslI CCNNNNNNNGG 2 cut(s) 211, 902
BsmFI GGGAC 1 cut(s) 814
BsmI GAATGC 1 cut(s) 798
BsnI GGCC 4 cut(s) 11, 243, 505, 600
BsoBI CYCGRG 1 cut(s) 896
Bsp1286I GDGCHC 2 cut(s) 316, 638
Bsp143I GATC 3 cut(s) 346, 667, 978
BspACI CCGC 4 cut(s) 12, 218, 859, 953
BspANI GGCC 4 cut(s) 11, 243, 505, 600
BspFNI CGCG 3 cut(s) 14, 79, 861
BspLI GGNNCC 2 cut(s) 63, 916
BspMI ACCTGC 1 cut(s) 290
BspT107I GGYRCC 1 cut(s) 61
BsrDI GCAATG 2 cut(s) 114, 549
BsrI ACTGG 2 cut(s) 159, 575
BssECI CCNNGG 1 cut(s) 601
BssMI GATC 3 cut(s) 346, 667, 978
Bst2UI CCWGG 2 cut(s) 240, 261
Bst4CI ACNGT 2 cut(s) 311, 828
Bst6I CTCTTC 2 cut(s) 33, 1010
BstC8I GCNNGC 1 cut(s) 714
BstDEI CTNAG 1 cut(s) 552
BstF5I GGATG 1 cut(s) 200
BstFNI CGCG 3 cut(s) 14, 79, 861
BstHHI GCGC 1 cut(s) 775
BstKTI GATC 3 cut(s) 349, 670, 981
BstMBI GATC 3 cut(s) 346, 667, 978
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 8 cut(s) 11, 131, 137, 146, 155, 416, 722, 923
BstNI CCWGG 2 cut(s) 240, 261
BstNSI RCATGY 1 cut(s) 194
BstSCI CCNGG 3 cut(s) 107, 238, 259
BstSLI GKGCMC 2 cut(s) 316, 638
BstUI CGCG 3 cut(s) 14, 79, 861
BstV1I GCAGC 7 cut(s) 34, 118, 121, 124, 202, 337, 454
BstV2I GAAGAC 2 cut(s) 110, 954
BstXI CCANNNNNNTGG 1 cut(s) 501
BstZI CGGCCG 1 cut(s) 9
BsuRI GGCC 4 cut(s) 11, 243, 505, 600
BtsCI GGATG 1 cut(s) 200
BtsI GCAGTG 2 cut(s) 153, 880
BtsIMutI CAGTG 4 cut(s) 153, 290, 775, 880
BveI ACCTGC 1 cut(s) 290
Cac8I GCNNGC 1 cut(s) 714
CfoI GCGC 1 cut(s) 775
Cfr13I GGNCC 1 cut(s) 598
CseI GACGC 1 cut(s) 850
Csp6I GTAC 5 cut(s) 62, 75, 379, 572, 805
CviAII CATG 4 cut(s) 191, 586, 865, 982
CviQI GTAC 5 cut(s) 62, 75, 379, 572, 805
DdeI CTNAG 1 cut(s) 552
DpnI GATC 3 cut(s) 348, 669, 980
DpnII GATC 3 cut(s) 346, 667, 978
EaeI YGGCCR 2 cut(s) 9, 241
EagI CGGCCG 1 cut(s) 9
Eam1104I CTCTTC 2 cut(s) 33, 1010
EarI CTCTTC 2 cut(s) 33, 1010
EclXI CGGCCG 1 cut(s) 9
Eco52I CGGCCG 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 266
Eco88I CYCGRG 1 cut(s) 896
EcoO109I RGGNCCY 1 cut(s) 598
EcoRII CCWGG 2 cut(s) 238, 259
FaeI CATG 4 cut(s) 194, 589, 868, 985
FaqI GGGAC 1 cut(s) 814
FatI CATG 4 cut(s) 190, 585, 864, 981
FbaI TGATCA 2 cut(s) 346, 667
Fnu4HI GCNGC 9 cut(s) 12, 48, 132, 135, 138, 216, 326, 443, 953
FokI GGATG 1 cut(s) 187
Fsp4HI GCNGC 9 cut(s) 12, 48, 132, 135, 138, 216, 326, 443, 953
FspBI CTAG 1 cut(s) 407
GlaI GCGC 1 cut(s) 774
GluI GCNGC 9 cut(s) 12, 48, 132, 135, 138, 216, 326, 443, 953
GsuI CTGGAG 2 cut(s) 27, 282
HaeIII GGCC 4 cut(s) 11, 243, 505, 600
HapII CCGG 2 cut(s) 109, 781
HgaI GACGC 1 cut(s) 850
HhaI GCGC 1 cut(s) 775
Hin1II CATG 4 cut(s) 194, 589, 868, 985
Hin6I GCGC 1 cut(s) 773
HinP1I GCGC 1 cut(s) 773
HincII GTYRAC 2 cut(s) 307, 515
HindII GTYRAC 2 cut(s) 307, 515
HinfI GANTC 3 cut(s) 179, 744, 974
HpaII CCGG 2 cut(s) 109, 781
HphI GGTGA 2 cut(s) 30, 370
Hpy166II GTNNAC 5 cut(s) 164, 307, 314, 515, 636
Hpy188I TCNGA 3 cut(s) 619, 967, 1010
Hpy188III TCNNGA 4 cut(s) 209, 741, 789, 896
Hpy8I GTNNAC 5 cut(s) 164, 307, 314, 515, 636
HpyAV CCTTC 4 cut(s) 393, 457, 516, 589
HpyCH4III ACNGT 2 cut(s) 311, 828
HpyCH4IV ACGT 1 cut(s) 73
HpyCH4V TGCA 9 cut(s) 50, 190, 314, 325, 610, 636, 684, 725, 1000
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 131, 137, 146, 155, 416, 722, 923
HpyF3I CTNAG 1 cut(s) 552
HpySE526I ACGT 1 cut(s) 73
Hsp92II CATG 4 cut(s) 194, 589, 868, 985
HspAI GCGC 1 cut(s) 773
KpnI GGTACC 1 cut(s) 65
Ksp22I TGATCA 2 cut(s) 346, 667
Kzo9I GATC 3 cut(s) 346, 667, 978
LmnI GCTCC 2 cut(s) 628, 914
Lsp1109I GCAGC 7 cut(s) 34, 118, 121, 124, 202, 337, 454
LweI GCATC 1 cut(s) 209
MaeI CTAG 1 cut(s) 407
MaeII ACGT 1 cut(s) 73
MaeIII GTNAC 2 cut(s) 19, 358
MalI GATC 3 cut(s) 348, 669, 980
MboI GATC 3 cut(s) 346, 667, 978
MboII GAAGA 8 cut(s) 20, 115, 305, 385, 434, 954, 997, 999
MhlI GDGCHC 2 cut(s) 316, 638
MlsI TGGCCA 1 cut(s) 243
MluCI AATT 4 cut(s) 341, 389, 621, 764
MluNI TGGCCA 1 cut(s) 243
MlyI GAGTC 1 cut(s) 983
MmeI TCCRAC 1 cut(s) 951
MnlI CCTC 9 cut(s) 9, 51, 130, 205, 216, 611, 619, 697, 802
Mox20I TGGCCA 1 cut(s) 243
MscI TGGCCA 1 cut(s) 243
MseI TTAA 3 cut(s) 69, 333, 438
MslI CAYNNNNRTG 3 cut(s) 189, 499, 590
Msp20I TGGCCA 1 cut(s) 243
MspA1I CMGCKG 2 cut(s) 47, 656
MspI CCGG 2 cut(s) 109, 781
MspR9I CCNGG 3 cut(s) 109, 240, 261
Mva1269I GAATGC 1 cut(s) 798
MvaI CCWGG 2 cut(s) 240, 261
MvnI CGCG 3 cut(s) 14, 79, 861
MwoI GCNNNNNNNGC 8 cut(s) 11, 131, 137, 146, 155, 416, 722, 923
NciI CCSGG 1 cut(s) 109
NdeII GATC 3 cut(s) 346, 667, 978
NlaIII CATG 4 cut(s) 194, 589, 868, 985
NlaIV GGNNCC 2 cut(s) 63, 916
NmuCI GTSAC 2 cut(s) 19, 358
NspI RCATGY 1 cut(s) 194
PaqCI CACCTGC 1 cut(s) 290
PctI GAATGC 1 cut(s) 798
PfeI GAWTC 2 cut(s) 179, 744
Pfl23II CGTACG 2 cut(s) 74, 804
PkrI GCNGC 9 cut(s) 13, 49, 133, 136, 139, 217, 327, 444, 954
PleI GAGTC 1 cut(s) 982
PpsI GAGTC 1 cut(s) 982
PshBI ATTAAT 1 cut(s) 333
Psp6I CCWGG 2 cut(s) 238, 259
PspGI CCWGG 2 cut(s) 238, 259
PspLI CGTACG 2 cut(s) 74, 804
PspN4I GGNNCC 2 cut(s) 63, 916
PspPI GGNCC 1 cut(s) 598
PsrI GAACNNNNNNTAC 2 cut(s) 555, 587
PvuII CAGCTG 2 cut(s) 47, 656
RsaI GTAC 5 cut(s) 63, 76, 380, 573, 806
RsaNI GTAC 5 cut(s) 62, 75, 379, 572, 805
RseI CAYNNNNRTG 3 cut(s) 189, 499, 590
SaqAI TTAA 3 cut(s) 69, 333, 438
SatI GCNGC 9 cut(s) 12, 48, 132, 135, 138, 216, 326, 443, 953
Sau3AI GATC 3 cut(s) 346, 667, 978
Sau96I GGNCC 1 cut(s) 598
SchI GAGTC 1 cut(s) 983
ScrFI CCNGG 3 cut(s) 109, 240, 261
SduI GDGCHC 2 cut(s) 316, 638
SfaNI GCATC 1 cut(s) 209
SmiMI CAYNNNNRTG 3 cut(s) 189, 499, 590
SmlI CTYRAG 1 cut(s) 207
SmoI CTYRAG 1 cut(s) 207
Sse9I AATT 4 cut(s) 341, 389, 621, 764
SsiI CCGC 4 cut(s) 12, 218, 859, 953
SspMI CTAG 1 cut(s) 407
StyD4I CCNGG 3 cut(s) 107, 238, 259
TaaI ACNGT 2 cut(s) 311, 828
TaiI ACGT 1 cut(s) 76
TaqI TCGA 5 cut(s) 270, 788, 842, 946, 977
TasI AATT 4 cut(s) 341, 389, 621, 764
TauI GCSGC 2 cut(s) 14, 955
TfiI GAWTC 2 cut(s) 179, 744
Tru1I TTAA 3 cut(s) 69, 333, 438
Tru9I TTAA 3 cut(s) 69, 333, 438
TscAI CASTG 4 cut(s) 160, 290, 775, 880
TseFI GTSAC 2 cut(s) 19, 358
TseI GCWGC 7 cut(s) 47, 131, 134, 137, 215, 325, 442
Tsp45I GTSAC 2 cut(s) 19, 358
TspDTI ATGAA 3 cut(s) 543, 574, 608
TspGWI ACGGA 2 cut(s) 653, 792
TspRI CASTG 4 cut(s) 160, 290, 775, 880
VneI GTGCAC 2 cut(s) 312, 634
VspI ATTAAT 1 cut(s) 333
XapI RAATTY 3 cut(s) 341, 389, 764
XceI RCATGY 1 cut(s) 194
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.