RchiOBHm_Chr4g0429651

Amidohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0015263
Physical Location & Seq
Reverse (-)
54417949 .. 54421199
3251 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39842

Sequence Viewer

Length: 987 bp
ATGGCAATGGCAGTGAGGCTAACACCTGTTCTGGCTCGGTTTAAACACTTTGCCTTCAGTTCAAGAGAAAACCAGTTCAGGGTCTCGGCTGCGGCAATGGCTACAGGTGGACCACAGAGCAGAACAAAAGTCATAGATTCACATCTCCATGTGTGGGCTTCACCTCAAGAGGCTGCTGATAAATATCCTTACTTTCCTGGGCAAGAACCCACTTTGCCTGGAGATGTCGATTTATTGCTTAAGTGCATGGAAGAAGCAGGTGTTGACGGTGCACTTATTGTGCAGCCTATTAATCACAAATTCGATCACTCTTTGGTGACAAGTGTGTTGAAGAAGTATCCATCTAAATTTGTTGGTTGTTGCCTTGCAAATCCAGCTGAAGATGCAAGTGGGGTTAAGCAGCTTGAACATCTTATTTTAAAGGATAACTATCGTGCTGTTCGCTTCAATCCATATTTATGGCCTTCTGGTCAAAAGATGACCAATGAAGTTGGAAAGGCAATGTTCTCCAAGGCGGGAGAACTTGGAGTACCAGTTGGCTTTATGTGTATGAAGGGCCTCGGTCTGCATATTTCAGAAATTGAGGAACTATGCACAGAGTTTCCTTCAACACTTGTATTACTTGATCATTTGGCTTTCTGCAAACCACCAATAAATGATGAGGAAAATGAGGCTTTCTCTGCACTTTTAAAGCTATCCAGATTTCCACAGGTCTATGTAAAAGTAAGTGCACTTTTCAGGGTGTCAAGAATGCCATTTCCATATGAAGATGTATCTCATACACTGTCCCAAGTTGTGTCAAGTTTTGGTGCTAACCGGGTCATGTGGGGCAGTGATTTTCCATTTGTTGTTGCTGAGTGTGGTTATGAAGGAGCAAAAGAAGCCGTTTCTTTGATTGCCAGTAAAATACCTCTCTCATCTTCAGAGTTGGAGTGGATCATGGGTGGAACAATCTTGCAACTCTTCCAGAATCAGTGGCTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_2

Protein Analysis

328

Amino Acids

36.29

Weight (kDa)

6.15

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_2 PF04909 45 - 322 2.6e-31 Amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35450
fragaria_vesca FvH4_4g23290 FvH4_4g23290
malus_domestica MD16G1123900.v1.1
prunus_persica Prupe.1G221700_v2.0.a1
pyrus_communis pycom16g10590
rosa_chinensis RchiOBHm_Chr4g0429651
rosa_laevigata RLG00000007055
rosa_multiflora Rmu_sc0006590.1_g000006
rosa_roxburghii Rroxscaffold_5G00371330
rosa_rugosa Rorug04G0237500
rosa_samantha Rh4AG293500 Rh4BG299300 Rh4CG315100 Rh4DG296400
rosa_wichuraiana Rw4G025400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 248
AciI CCGC 2 cut(s) 92, 515
AclWI GGATC 1 cut(s) 944
AcsI RAATTY 2 cut(s) 299, 347
AcuI CTGAAG 3 cut(s) 40, 399, 906
AfaI GTAC 1 cut(s) 531
AfiI CCNNNNNNNGG 2 cut(s) 79, 154
AflII CTTAAG 1 cut(s) 239
AgsI TTSAA 5 cut(s) 63, 331, 407, 448, 609
AjnI CCWGG 2 cut(s) 196, 217
AluBI AGCT 3 cut(s) 377, 403, 694
AluI AGCT 3 cut(s) 377, 403, 694
Alw21I GWGCWC 2 cut(s) 274, 733
Alw26I GTCTC 1 cut(s) 88
Alw44I GTGCAC 2 cut(s) 270, 729
AlwI GGATC 1 cut(s) 944
AoxI GGCC 2 cut(s) 461, 556
ApaLI GTGCAC 2 cut(s) 270, 729
ApeKI GCWGC 4 cut(s) 89, 173, 283, 400
ApoI RAATTY 2 cut(s) 299, 347
AseI ATTAAT 1 cut(s) 291
AspS9I GGNCC 2 cut(s) 110, 556
AsuC2I CCSGG 1 cut(s) 818
AsuHPI GGTGA 2 cut(s) 153, 328
AvaII GGWCC 1 cut(s) 110
BaeGI GKGCMC 2 cut(s) 274, 733
Bbv12I GWGCWC 2 cut(s) 274, 733
BbvI GCAGC 4 cut(s) 76, 160, 295, 412
BccI CCATC 1 cut(s) 349
BceAI ACGGC 1 cut(s) 869
BciT130I CCWGG 2 cut(s) 198, 219
BciVI GTATCC 1 cut(s) 348
BclI TGATCA 1 cut(s) 625
BcnI CCSGG 1 cut(s) 818
BcoDI GTCTC 1 cut(s) 88
BfmI CTRYAG 1 cut(s) 102
BfrI CTTAAG 1 cut(s) 239
BfuAI ACCTGC 1 cut(s) 248
BfuI GTATCC 1 cut(s) 348
BisI GCNGC 5 cut(s) 90, 93, 174, 284, 401
BlsI GCNGC 5 cut(s) 91, 94, 175, 285, 402
Bme1390I CCNGG 3 cut(s) 198, 219, 818
Bme18I GGWCC 1 cut(s) 110
BmgT120I GGNCC 2 cut(s) 110, 556
BmrFI CCNGG 3 cut(s) 198, 219, 818
BmsI GCATC 1 cut(s) 373
BplI GAGNNNNNCTC 2 cut(s) 662, 694
BpmI CTGGAG 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 150
BpuMI CCSGG 1 cut(s) 818
BsaBI GATNNNNATC 3 cut(s) 141, 183, 429
BsaI GGTCTC 1 cut(s) 88
BsaJI CCNNGG 3 cut(s) 197, 510, 559
BsaXI ACNNNNNCTCC 2 cut(s) 519, 549
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 154
Bse1I ACTGG 3 cut(s) 73, 533, 900
Bse3DI GCAATG 3 cut(s) 12, 102, 507
Bse8I GATNNNNATC 3 cut(s) 141, 183, 429
BseBI CCWGG 2 cut(s) 198, 219
BseDI CCNNGG 3 cut(s) 197, 510, 559
BseJI GATNNNNATC 3 cut(s) 141, 183, 429
BseLI CCNNNNNNNGG 2 cut(s) 79, 154
BseMI GCAATG 3 cut(s) 12, 102, 507
BseMII CTCAG 1 cut(s) 846
BseNI ACTGG 3 cut(s) 73, 533, 900
BseSI GKGCMC 2 cut(s) 274, 733
BseXI GCAGC 4 cut(s) 76, 160, 295, 412
BsgI GTGCAG 2 cut(s) 302, 666
BshFI GGCC 2 cut(s) 463, 558
BsiHKAI GWGCWC 2 cut(s) 274, 733
BsiSI CCGG 1 cut(s) 817
BslFI GGGAC 1 cut(s) 772
BslI CCNNNNNNNGG 2 cut(s) 79, 154
BsmAI GTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 772
BsmI GAATGC 1 cut(s) 756
BsnI GGCC 2 cut(s) 463, 558
Bso31I GGTCTC 1 cut(s) 88
Bsp1286I GDGCHC 2 cut(s) 274, 733
Bsp143I GATC 3 cut(s) 304, 625, 936
BspACI CCGC 2 cut(s) 92, 515
BspANI GGCC 2 cut(s) 463, 558
BspCNI CTCAG 1 cut(s) 847
BspMI ACCTGC 1 cut(s) 248
BspPI GGATC 1 cut(s) 944
BspTI CTTAAG 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 88
BsrDI GCAATG 3 cut(s) 12, 102, 507
BsrI ACTGG 3 cut(s) 73, 533, 900
BssECI CCNNGG 3 cut(s) 197, 510, 559
BssMI GATC 3 cut(s) 304, 625, 936
BssT1I CCWWGG 1 cut(s) 510
Bst2UI CCWGG 2 cut(s) 198, 219
Bst4CI ACNGT 2 cut(s) 269, 786
Bst6I CTCTTC 1 cut(s) 968
BstAFI CTTAAG 1 cut(s) 239
BstDEI CTNAG 1 cut(s) 855
BstKTI GATC 3 cut(s) 307, 628, 939
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 3 cut(s) 304, 625, 936
BstMWI GCNNNNNNNGC 5 cut(s) 98, 374, 383, 680, 881
BstNI CCWGG 2 cut(s) 198, 219
BstSCI CCNGG 3 cut(s) 196, 217, 816
BstSFI CTRYAG 1 cut(s) 102
BstSLI GKGCMC 2 cut(s) 274, 733
BstV1I GCAGC 4 cut(s) 76, 160, 295, 412
BstXI CCANNNNNNTGG 1 cut(s) 459
BsuI GTATCC 1 cut(s) 348
BsuRI GGCC 2 cut(s) 463, 558
BtsI GCAGTG 2 cut(s) 18, 838
BtsIMutI CAGTG 4 cut(s) 18, 782, 838, 980
BveI ACCTGC 1 cut(s) 248
Cfr13I GGNCC 2 cut(s) 110, 556
Csp6I GTAC 1 cut(s) 530
CviAII CATG 4 cut(s) 149, 247, 823, 940
CviQI GTAC 1 cut(s) 530
DdeI CTNAG 1 cut(s) 855
DpnI GATC 3 cut(s) 306, 627, 938
DpnII GATC 3 cut(s) 304, 625, 936
DraI TTTAAA 3 cut(s) 43, 420, 690
Eam1104I CTCTTC 1 cut(s) 968
EarI CTCTTC 1 cut(s) 968
Eco130I CCWWGG 1 cut(s) 510
Eco31I GGTCTC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 110
Eco57I CTGAAG 3 cut(s) 40, 399, 906
EcoO109I RGGNCCY 1 cut(s) 556
EcoRII CCWGG 2 cut(s) 196, 217
EcoT14I CCWWGG 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 4 cut(s) 152, 250, 826, 943
FaqI GGGAC 1 cut(s) 772
FatI CATG 4 cut(s) 148, 246, 822, 939
FauI CCCGC 1 cut(s) 508
FauNDI CATATG 1 cut(s) 763
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 5 cut(s) 90, 93, 174, 284, 401
Fsp4HI GCNGC 5 cut(s) 90, 93, 174, 284, 401
GluI GCNGC 5 cut(s) 90, 93, 174, 284, 401
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 2 cut(s) 463, 558
HapII CCGG 1 cut(s) 817
Hin1II CATG 4 cut(s) 152, 250, 826, 943
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 2 cut(s) 137, 970
HpaII CCGG 1 cut(s) 817
HphI GGTGA 2 cut(s) 153, 328
Hpy166II GTNNAC 4 cut(s) 110, 265, 272, 731
Hpy188I TCNGA 2 cut(s) 577, 925
Hpy188III TCNNGA 5 cut(s) 63, 167, 699, 747, 967
Hpy8I GTNNAC 4 cut(s) 110, 265, 272, 731
HpyAV CCTTC 5 cut(s) 64, 474, 547, 615, 863
HpyCH4III ACNGT 2 cut(s) 269, 786
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 374, 383, 680, 881
HpyF3I CTNAG 1 cut(s) 855
Hsp92II CATG 4 cut(s) 152, 250, 826, 943
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 3 cut(s) 304, 625, 936
LmnI GCTCC 1 cut(s) 872
Lsp1109I GCAGC 4 cut(s) 76, 160, 295, 412
LweI GCATC 1 cut(s) 373
MaeIII GTNAC 1 cut(s) 316
MalI GATC 3 cut(s) 306, 627, 938
MboI GATC 3 cut(s) 304, 625, 936
MboII GAAGA 6 cut(s) 263, 343, 392, 779, 912, 955
MhlI GDGCHC 2 cut(s) 274, 733
MluCI AATT 3 cut(s) 299, 347, 579
MmeI TCCRAC 2 cut(s) 472, 909
MnlI CCTC 8 cut(s) 9, 163, 174, 569, 577, 655, 664, 921
MseI TTAA 6 cut(s) 42, 240, 291, 396, 419, 689
MslI CAYNNNNRTG 2 cut(s) 147, 457
MspA1I CMGCKG 1 cut(s) 377
MspCI CTTAAG 1 cut(s) 239
MspI CCGG 1 cut(s) 817
MspR9I CCNGG 3 cut(s) 198, 219, 818
MssI GTTTAAAC 1 cut(s) 43
Mva1269I GAATGC 1 cut(s) 756
MvaI CCWGG 2 cut(s) 198, 219
MwoI GCNNNNNNNGC 5 cut(s) 98, 374, 383, 680, 881
NciI CCSGG 1 cut(s) 818
NdeI CATATG 1 cut(s) 763
NdeII GATC 3 cut(s) 304, 625, 936
NlaIII CATG 4 cut(s) 152, 250, 826, 943
NmeAIII GCCGAG 1 cut(s) 65
NmuCI GTSAC 1 cut(s) 316
PaqCI CACCTGC 1 cut(s) 248
PctI GAATGC 1 cut(s) 756
PfeI GAWTC 2 cut(s) 137, 970
PkrI GCNGC 5 cut(s) 91, 94, 175, 285, 402
PmeI GTTTAAAC 1 cut(s) 43
PshBI ATTAAT 1 cut(s) 291
Psp6I CCWGG 2 cut(s) 196, 217
PspGI CCWGG 2 cut(s) 196, 217
PspPI GGNCC 2 cut(s) 110, 556
PsrI GAACNNNNNNTAC 2 cut(s) 513, 545
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
RseI CAYNNNNRTG 2 cut(s) 147, 457
SaqAI TTAA 6 cut(s) 42, 240, 291, 396, 419, 689
SatI GCNGC 5 cut(s) 90, 93, 174, 284, 401
Sau3AI GATC 3 cut(s) 304, 625, 936
Sau96I GGNCC 2 cut(s) 110, 556
ScrFI CCNGG 3 cut(s) 198, 219, 818
SduI GDGCHC 2 cut(s) 274, 733
SetI ASST 9 cut(s) 28, 109, 166, 262, 379, 405, 696, 714, 913
SfaNI GCATC 1 cut(s) 373
SfcI CTRYAG 1 cut(s) 102
SinI GGWCC 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 147, 457
SmlI CTYRAG 2 cut(s) 165, 239
SmoI CTYRAG 2 cut(s) 165, 239
Sse9I AATT 3 cut(s) 299, 347, 579
SsiI CCGC 2 cut(s) 92, 515
StyD4I CCNGG 3 cut(s) 196, 217, 816
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 2 cut(s) 269, 786
TaqI TCGA 2 cut(s) 228, 303
TaqII GACCGA 1 cut(s) 551
TasI AATT 3 cut(s) 299, 347, 579
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 2 cut(s) 137, 970
Tru1I TTAA 6 cut(s) 42, 240, 291, 396, 419, 689
Tru9I TTAA 6 cut(s) 42, 240, 291, 396, 419, 689
TscAI CASTG 4 cut(s) 18, 789, 838, 980
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 4 cut(s) 89, 173, 283, 400
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 4 cut(s) 501, 566, 780, 882
TspRI CASTG 4 cut(s) 18, 789, 838, 980
Vha464I CTTAAG 1 cut(s) 239
VneI GTGCAC 2 cut(s) 270, 729
VpaK11BI GGWCC 1 cut(s) 110
VspI ATTAAT 1 cut(s) 291
XapI RAATTY 2 cut(s) 299, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.