Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006976

Physical Location & Seq

Genomic Coordinates Forward (+)

14959386 .. 14960828

Gene Structure 1443 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G35615.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGGCAACCCAAATTCTTCTCTGTTTCTTCCTCTTCTTCTCGGTGACTCTCTCTTCATCGGGTCACCCAAAAAACTTCAGTGTTGAGCTGATCCACCGTGACTCTCCCCTTTCCCCAATCTACAACCCACAAATCACCGTCACTGATCGCCTCAACGCTGCTTTCCTCCGCTCAGTCTCTCGTTCCCGCCGCTTCAACCACCAACTATCCCAAACCGATCTCCAATCCGGTTTAATCGGAGCAGACGGCGAGTTTTTCATGAGTATCACCATCGGTACTCCACCAATAAAGGTTTTTGCGATCGCTGACACAGGAAGTGACCTAACTTGGGTCCAATGTAAGCCTTGTCAACAATGTTACAAAGAAAACGGTCCAATCTTCGACAAGAAGAAATCCTCTACTTATAAGAGCGAGCCTTGTGACTCACGTAACTGCCAGGCCTTGTCCAGTACGGAACGGGGATGCGATGAATCCAACAATATATGCAAGTACCGTTACTCTTACGGTGATCAGTCGTTCAGCAAAGGAGACGTTGCCACGGAAACTGTCTCTATTGACTCTGCTTCAGGGTCCCCTGTTTCTTTTCCTGGTACTGTCTTCGGTTGTGGCTACAATAACGGTGGTACCTTCGACGAGACTGGTTCTGGGATTATCGGTCTAGGTGGTGGTCATTTATCACTAATCTCCCAGCTCGGTTCGTCGATTTCGAAGAAATTTTCTTATTGCTTGTCTCACAAGTCAGCTACCACGAATGGCACAAGCGTCATAAACCTAGGCACTAACTCGATTCCTTCAAGTCTAAGCAAAGACTCCGGCGTGGTATCAACTCCGTTGGTCGATAAAGAGCCTCTTACTTACTACTACTTGACGCTTGAAGCCATCTCCGTCGGGAAGAAAAAGATTCCTTACACCGGAAGCTCGTATAACCCAAACGACGACGGAATACTCTCGGAGACATCTGGAAACATCATCATCGACTCTGGAACGACACTGACCCTCCTCGAAGCTGGTTTCTTTGACAAATTTAGCTCGGCGGTGGAGGAATCAGTGACCGGAGCCAAGCGCGTGAGTGATCCACAAGGGCTTTTGTCTCACTGTTTCAAATCCGGAAGTGCAGAGATCGGTTTGCCGGAGATAACGGTGCATTTCACGGGTGCAGATGTGAGGCTGAGTCCGATCAACGCATTCGTGAAACTGAGTGAAGACATGGTTTGCTTGAGTATGGTTCCGACCACCGAGGTTGCCATCTATGGGAACTTTGCTCAGATGGATTTCCTAGTTGGGTATGACTTGGAGACGAGGACGGTGTCGTTTCAACACATGGATTGCTCTGCGAATTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0005575 GO:0005576 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0010310 GO:0010337 GO:0010565 GO:0010817 GO:0016787 GO:0019222 GO:0019538 GO:0030163 GO:0031323 GO:0032350 GO:0042742 GO:0043170 GO:0043207 GO:0044238 GO:0048046 GO:0050789 GO:0050794 GO:0050896 GO:0051193 GO:0051704 GO:0051707 GO:0062012 GO:0065007 GO:0065008 GO:0070001 GO:0070011 GO:0071704 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:2000377

KEGG Pathways

Metabolic & Signaling

ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152

Pfam Domains

Protein Families

TAXi_C TAXi_N

Protein Analysis

447

Amino Acids

48.09

Weight (kDa)

5.33

Isoelectric Point (pI)

34.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TAXi_N	PF14543	85 - 259	2.1e-55	Xylanase inhibitor N-terminal
Asp	PF00026	85 - 438	3.4e-15	Eukaryotic aspartyl protease
TAXi_C	PF14541	287 - 439	5.6e-25	Xylanase inhibitor C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006976)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31450 AT2G35615
fragaria_vesca	FvH4_1g21300 FvH4_5g24370
malus_domestica	MD06G1021800.v1.1 MD16G1288400.v1.1
prunus_persica	Prupe.5G017100_v2.0.a1
pyrus_communis	pycom06g01570
rosa_chinensis	RchiOBHm_Chr2g0115131 RchiOBHm_Chr7g0214751
rosa_laevigata	RLG00000002724 RLG00000018161
rosa_roxburghii	Rroxscaffold_2G00130610 Rroxscaffold_3G00245120
rosa_rugosa	Rorug02G0193900
rosa_samantha	Rh2AG252400 Rh2BG261900 Rh2CG256900 Rh2DG260600 Rh7AG283700 Rh7BG274400 Rh7CG303100 Rh7DG287100
rosa_wichuraiana	Rw2G019680 Rw7G024190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	405
Acc65I	GGTACC	1 cut(s)	623
AccB1I	GGYRCC	1 cut(s)	623
AccBSI	CCGCTC	1 cut(s)	171
AccII	CGCG	1 cut(s)	1065
AccIII	TCCGGA	1 cut(s)	1106
AciI	CCGC	4 cut(s)	169, 187, 190, 1034
AclWI	GGATC	2 cut(s)	85, 1067
AcsI	RAATTY	4 cut(s)	12, 713, 1022, 1336
AcuI	CTGAAG	2 cut(s)	61, 549
AfaI	GTAC	5 cut(s)	277, 451, 491, 592, 625
AfiI	CCNNNNNNNGG	3 cut(s)	328, 911, 1251
AgsI	TTSAA	5 cut(s)	196, 795, 875, 1102, 1316
AjnI	CCWGG	2 cut(s)	435, 586
AluBI	AGCT	6 cut(s)	88, 691, 743, 918, 1007, 1029
AluI	AGCT	6 cut(s)	88, 691, 743, 918, 1007, 1029
Alw26I	GTCTC	8 cut(s)	181, 522, 553, 629, 735, 947, 1095, 1289
AlwI	GGATC	2 cut(s)	85, 1067
Aor13HI	TCCGGA	1 cut(s)	1106
AoxI	GGCC	1 cut(s)	438
ApeKI	GCWGC	1 cut(s)	158
ApoI	RAATTY	4 cut(s)	12, 713, 1022, 1336
AsiSI	GCGATCGC	1 cut(s)	303
Asp718I	GGTACC	1 cut(s)	623
AspA2I	CCTAGG	1 cut(s)	772
AspLEI	GCGC	1 cut(s)	1065
AspS9I	GGNCC	3 cut(s)	331, 371, 570
AsuHPI	GGTGA	5 cut(s)	55, 56, 127, 259, 518
AsuII	TTCGAA	1 cut(s)	707
AvaII	GGWCC	3 cut(s)	331, 371, 570
AvrII	CCTAGG	1 cut(s)	772
BaeI	ACNNNNGTAYC	2 cut(s)	607, 640
BanI	GGYRCC	1 cut(s)	623
BbsI	GAAGAC	2 cut(s)	589, 1209
BbvI	GCAGC	1 cut(s)	145
BccI	CCATC	4 cut(s)	278, 887, 1253, 1261
BceAI	ACGGC	1 cut(s)	262
BciT130I	CCWGG	2 cut(s)	437, 588
BclI	TGATCA	1 cut(s)	508
BcoDI	GTCTC	8 cut(s)	181, 522, 553, 629, 735, 947, 1095, 1289
BfaI	CTAG	3 cut(s)	659, 773, 1277
BisI	GCNGC	2 cut(s)	159, 190
BlnI	CCTAGG	1 cut(s)	772
BlsI	GCNGC	2 cut(s)	160, 191
Bme1390I	CCNGG	2 cut(s)	437, 588
Bme18I	GGWCC	3 cut(s)	331, 371, 570
BmgT120I	GGNCC	3 cut(s)	331, 371, 570
BmiI	GGNNCC	6 cut(s)	332, 571, 572, 625, 1057, 1227
BmrFI	CCNGG	2 cut(s)	437, 588
BmsI	GCATC	1 cut(s)	452
BpiI	GAAGAC	2 cut(s)	589, 1209
Bpu14I	TTCGAA	1 cut(s)	707
BpuEI	CTTGAG	1 cut(s)	1237
BsaAI	YACGTR	1 cut(s)	428
BsaJI	CCNNGG	3 cut(s)	537, 772, 1236
BsaWI	WCCGGW	4 cut(s)	227, 911, 1052, 1106
BsaXI	ACNNNNNCTCC	2 cut(s)	981, 1011
Bsc4I	CCNNNNNNNGG	3 cut(s)	328, 911, 1251
Bse1I	ACTGG	2 cut(s)	447, 643
BseAI	TCCGGA	1 cut(s)	1106
BseBI	CCWGG	2 cut(s)	437, 588
BseDI	CCNNGG	3 cut(s)	537, 772, 1236
BseGI	GGATG	1 cut(s)	467
BseLI	CCNNNNNNNGG	3 cut(s)	328, 911, 1251
BseMII	CTCAG	4 cut(s)	186, 1160, 1187, 1277
BseNI	ACTGG	2 cut(s)	447, 643
BseRI	GAGGAG	1 cut(s)	989
BseXI	GCAGC	1 cut(s)	145
BseYI	CCCAGC	1 cut(s)	687
BsgI	GTGCAG	2 cut(s)	1134, 1176
Bsh1236I	CGCG	1 cut(s)	1065
Bsh1285I	CGRYCG	1 cut(s)	303
BshFI	GGCC	1 cut(s)	440
BshNI	GGYRCC	1 cut(s)	623
BsiEI	CGRYCG	1 cut(s)	303
BsiSI	CCGG	6 cut(s)	228, 813, 912, 1053, 1107, 1130
BslFI	GGGAC	1 cut(s)	556
BslI	CCNNNNNNNGG	3 cut(s)	328, 911, 1251
BsmAI	GTCTC	8 cut(s)	181, 522, 553, 629, 735, 947, 1095, 1289
BsmBI	CGTCTC	2 cut(s)	522, 1289
BsmFI	GGGAC	1 cut(s)	556
BsmI	GAATGC	1 cut(s)	1184
BsnI	GGCC	1 cut(s)	440
Bsp119I	TTCGAA	1 cut(s)	707
Bsp13I	TCCGGA	1 cut(s)	1106
Bsp143I	GATC	8 cut(s)	90, 145, 217, 300, 508, 1072, 1119, 1176
BspACI	CCGC	4 cut(s)	169, 187, 190, 1034
BspANI	GGCC	1 cut(s)	440
BspCNI	CTCAG	4 cut(s)	185, 1161, 1188, 1276
BspEI	TCCGGA	1 cut(s)	1106
BspFNI	CGCG	1 cut(s)	1065
BspHI	TCATGA	1 cut(s)	258
BspLI	GGNNCC	6 cut(s)	332, 571, 572, 625, 1057, 1227
BspPI	GGATC	2 cut(s)	85, 1067
BspT104I	TTCGAA	1 cut(s)	707
BspT107I	GGYRCC	1 cut(s)	623
BsrBI	CCGCTC	1 cut(s)	171
BsrI	ACTGG	2 cut(s)	447, 643
BssECI	CCNNGG	3 cut(s)	537, 772, 1236
BssMI	GATC	8 cut(s)	90, 145, 217, 300, 508, 1072, 1119, 1176
BssT1I	CCWWGG	1 cut(s)	772
Bst2UI	CCWGG	2 cut(s)	437, 588
Bst6I	CTCTTC	2 cut(s)	38, 58
BstBAI	YACGTR	1 cut(s)	428
BstBI	TTCGAA	1 cut(s)	707
BstC8I	GCNNGC	1 cut(s)	413
BstDEI	CTNAG	5 cut(s)	172, 800, 1169, 1196, 1263
BstDSI	CCRYGG	1 cut(s)	537
BstEII	GGTNACC	1 cut(s)	62
BstF5I	GGATG	1 cut(s)	467
BstFNI	CGCG	1 cut(s)	1065
BstHHI	GCGC	1 cut(s)	1065
BstKTI	GATC	8 cut(s)	93, 148, 220, 303, 511, 1075, 1122, 1179
BstMAI	GTCTC	8 cut(s)	181, 522, 553, 629, 735, 947, 1095, 1289
BstMBI	GATC	8 cut(s)	90, 145, 217, 300, 508, 1072, 1119, 1176
BstMCI	CGRYCG	1 cut(s)	303
BstNI	CCWGG	2 cut(s)	437, 588
BstPI	GGTNACC	1 cut(s)	62
BstSCI	CCNGG	2 cut(s)	435, 586
BstUI	CGCG	1 cut(s)	1065
BstV1I	GCAGC	1 cut(s)	145
BstV2I	GAAGAC	2 cut(s)	589, 1209
BsuRI	GGCC	1 cut(s)	440
BtgI	CCRYGG	1 cut(s)	537
BtgZI	GCGATG	1 cut(s)	480
BtsCI	GGATG	1 cut(s)	467
BtsIMutI	CAGTG	5 cut(s)	85, 141, 989, 1053, 1093
Cac8I	GCNNGC	1 cut(s)	413
CciI	TCATGA	1 cut(s)	258
CfoI	GCGC	1 cut(s)	1065
Cfr13I	GGNCC	3 cut(s)	331, 371, 570
CseI	GACGC	2 cut(s)	751, 877
Csp6I	GTAC	5 cut(s)	276, 450, 490, 591, 624
CspCI	CAANNNNNGTGG	4 cut(s)	601, 636, 1222, 1257
CviAII	CATG	3 cut(s)	259, 1207, 1321
CviQI	GTAC	5 cut(s)	276, 450, 490, 591, 624
DdeI	CTNAG	5 cut(s)	172, 800, 1169, 1196, 1263
DpnI	GATC	8 cut(s)	92, 147, 219, 302, 510, 1074, 1121, 1178
DpnII	GATC	8 cut(s)	90, 145, 217, 300, 508, 1072, 1119, 1176
Eam1104I	CTCTTC	2 cut(s)	38, 58
EarI	CTCTTC	2 cut(s)	38, 58
Eco130I	CCWWGG	1 cut(s)	772
Eco147I	AGGCCT	1 cut(s)	440
Eco47I	GGWCC	3 cut(s)	331, 371, 570
Eco57I	CTGAAG	2 cut(s)	61, 549
Eco91I	GGTNACC	1 cut(s)	62
EcoO109I	RGGNCCY	1 cut(s)	570
EcoO65I	GGTNACC	1 cut(s)	62
EcoRII	CCWGG	2 cut(s)	435, 586
EcoT14I	CCWWGG	1 cut(s)	772
ErhI	CCWWGG	1 cut(s)	772
Esp3I	CGTCTC	2 cut(s)	522, 1289
FaeI	CATG	3 cut(s)	262, 1210, 1324
FalI	AAGNNNNNCTT	2 cut(s)	834, 866
FaqI	GGGAC	1 cut(s)	556
FatI	CATG	3 cut(s)	258, 1206, 1320
FauI	CCCGC	1 cut(s)	194
FbaI	TGATCA	1 cut(s)	508
Fnu4HI	GCNGC	2 cut(s)	159, 190
FokI	GGATG	1 cut(s)	474
Fsp4HI	GCNGC	2 cut(s)	159, 190
FspBI	CTAG	3 cut(s)	659, 773, 1277
GlaI	GCGC	1 cut(s)	1064
GluI	GCNGC	2 cut(s)	159, 190
GsaI	CCCAGC	1 cut(s)	691
HaeIII	GGCC	1 cut(s)	440
HapII	CCGG	6 cut(s)	228, 813, 912, 1053, 1107, 1130
HgaI	GACGC	2 cut(s)	751, 877
HhaI	GCGC	1 cut(s)	1065
Hin1II	CATG	3 cut(s)	262, 1210, 1324
Hin6I	GCGC	1 cut(s)	1063
HinP1I	GCGC	1 cut(s)	1063
HincII	GTYRAC	1 cut(s)	350
HindII	GTYRAC	1 cut(s)	350
HpaII	CCGG	6 cut(s)	228, 813, 912, 1053, 1107, 1130
HphI	GGTGA	5 cut(s)	55, 56, 127, 259, 518
Hpy166II	GTNNAC	1 cut(s)	350
Hpy188I	TCNGA	5 cut(s)	239, 952, 1176, 1230, 1266
Hpy188III	TCNNGA	6 cut(s)	259, 889, 960, 981, 1107, 1189
Hpy8I	GTNNAC	1 cut(s)	350
Hpy99I	CGWCG	5 cut(s)	635, 703, 890, 938, 941
HpyAV	CCTTC	2 cut(s)	637, 801
HpyCH4IV	ACGT	2 cut(s)	427, 531
HpyCH4V	TGCA	4 cut(s)	486, 1115, 1144, 1157
HpyF3I	CTNAG	5 cut(s)	172, 800, 1169, 1196, 1263
HpySE526I	ACGT	2 cut(s)	427, 531
Hsp92II	CATG	3 cut(s)	262, 1210, 1324
HspAI	GCGC	1 cut(s)	1063
KflI	GGGWCCC	1 cut(s)	570
Kpn2I	TCCGGA	1 cut(s)	1106
KpnI	GGTACC	1 cut(s)	627
Ksp22I	TGATCA	1 cut(s)	508
Kzo9I	GATC	8 cut(s)	90, 145, 217, 300, 508, 1072, 1119, 1176
LmnI	GCTCC	2 cut(s)	239, 1055
Lsp1109I	GCAGC	1 cut(s)	145
LweI	GCATC	1 cut(s)	452
MaeI	CTAG	3 cut(s)	659, 773, 1277
MaeII	ACGT	2 cut(s)	427, 531
MalI	GATC	8 cut(s)	92, 147, 219, 302, 510, 1074, 1121, 1178
MbiI	CCGCTC	1 cut(s)	171
MboI	GATC	8 cut(s)	90, 145, 217, 300, 508, 1072, 1119, 1176
MluCI	AATT	4 cut(s)	12, 713, 1022, 1336
MlyI	GAGTC	7 cut(s)	40, 95, 416, 551, 803, 971, 1180
MmeI	TCCRAC	2 cut(s)	498, 1253
MroI	TCCGGA	1 cut(s)	1106
MseI	TTAA	1 cut(s)	233
MspI	CCGG	6 cut(s)	228, 813, 912, 1053, 1107, 1130
MspR9I	CCNGG	2 cut(s)	437, 588
Mva1269I	GAATGC	1 cut(s)	1184
MvaI	CCWGG	2 cut(s)	437, 588
MvnI	CGCG	1 cut(s)	1065
NdeII	GATC	8 cut(s)	90, 145, 217, 300, 508, 1072, 1119, 1176
NlaIII	CATG	3 cut(s)	262, 1210, 1324
NlaIV	GGNNCC	6 cut(s)	332, 571, 572, 625, 1057, 1227
NmeAIII	GCCGAG	1 cut(s)	1010
NmuCI	GTSAC	7 cut(s)	43, 62, 98, 139, 317, 419, 1048
NspV	TTCGAA	1 cut(s)	707
PagI	TCATGA	1 cut(s)	258
PceI	AGGCCT	1 cut(s)	440
PcsI	WCGNNNNNNNCGW	1 cut(s)	704
PctI	GAATGC	1 cut(s)	1184
PfeI	GAWTC	4 cut(s)	470, 787, 901, 1043
PflFI	GACNNNGTC	1 cut(s)	1306
PkrI	GCNGC	2 cut(s)	160, 191
Ple19I	CGATCG	1 cut(s)	303
PleI	GAGTC	7 cut(s)	40, 95, 416, 551, 803, 971, 1179
PpsI	GAGTC	7 cut(s)	40, 95, 416, 551, 803, 971, 1179
Ppu21I	YACGTR	1 cut(s)	428
PpuMI	RGGWCCY	1 cut(s)	570
PsiI	TTATAA	1 cut(s)	405
Psp5II	RGGWCCY	1 cut(s)	570
Psp6I	CCWGG	2 cut(s)	435, 586
PspEI	GGTNACC	1 cut(s)	62
PspFI	CCCAGC	1 cut(s)	687
PspGI	CCWGG	2 cut(s)	435, 586
PspN4I	GGNNCC	6 cut(s)	332, 571, 572, 625, 1057, 1227
PspPI	GGNCC	3 cut(s)	331, 371, 570
PspPPI	RGGWCCY	1 cut(s)	570
PsyI	GACNNNGTC	1 cut(s)	1306
PvuI	CGATCG	1 cut(s)	303
RgaI	GCGATCGC	1 cut(s)	303
RsaI	GTAC	5 cut(s)	277, 451, 491, 592, 625
RsaNI	GTAC	5 cut(s)	276, 450, 490, 591, 624
SaqAI	TTAA	1 cut(s)	233
SatI	GCNGC	2 cut(s)	159, 190
Sau3AI	GATC	8 cut(s)	90, 145, 217, 300, 508, 1072, 1119, 1176
Sau96I	GGNCC	3 cut(s)	331, 371, 570
SchI	GAGTC	7 cut(s)	40, 95, 416, 551, 803, 971, 1180
ScrFI	CCNGG	2 cut(s)	437, 588
SfaAI	GCGATCGC	1 cut(s)	303
SfaNI	GCATC	1 cut(s)	452
SfuI	TTCGAA	1 cut(s)	707
SgfI	GCGATCGC	1 cut(s)	303
SinI	GGWCC	3 cut(s)	331, 371, 570
SmlI	CTYRAG	1 cut(s)	1216
SmoI	CTYRAG	1 cut(s)	1216
Sse9I	AATT	4 cut(s)	12, 713, 1022, 1336
SseBI	AGGCCT	1 cut(s)	440
SsiI	CCGC	4 cut(s)	169, 187, 190, 1034
SspMI	CTAG	3 cut(s)	659, 773, 1277
StuI	AGGCCT	1 cut(s)	440
StyD4I	CCNGG	2 cut(s)	435, 586
StyI	CCWWGG	1 cut(s)	772
TaiI	ACGT	2 cut(s)	430, 534
TaqI	TCGA	8 cut(s)	381, 630, 701, 707, 785, 837, 975, 1002
TaqII	GACCGA	1 cut(s)	644
TasI	AATT	4 cut(s)	12, 713, 1022, 1336
TauI	GCSGC	1 cut(s)	192
TfiI	GAWTC	4 cut(s)	470, 787, 901, 1043
Tru1I	TTAA	1 cut(s)	233
Tru9I	TTAA	1 cut(s)	233
TscAI	CASTG	5 cut(s)	85, 148, 996, 1053, 1100
TseFI	GTSAC	7 cut(s)	43, 62, 98, 139, 317, 419, 1048
TseI	GCWGC	1 cut(s)	158
Tsp45I	GTSAC	7 cut(s)	43, 62, 98, 139, 317, 419, 1048
TspDTI	ATGAA	3 cut(s)	45, 247, 483
TspGWI	ACGGA	5 cut(s)	467, 554, 819, 874, 954
TspRI	CASTG	5 cut(s)	85, 148, 996, 1053, 1100
Tth111I	GACNNNGTC	1 cut(s)	1306
VpaK11BI	GGWCC	3 cut(s)	331, 371, 570
XapI	RAATTY	4 cut(s)	12, 713, 1022, 1336
XmaJI	CCTAGG	1 cut(s)	772
XspI	CTAG	3 cut(s)	659, 773, 1277

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.