AT2G35780

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004331
Physical Location & Seq
Reverse (-)
15037579 .. 15040298
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35780.1

Sequence Viewer

Length: 1359 bp
ATGGCTCGACTCCTTCTCCTCTTCTTCTTCTTCCTTATTCTACTCCATTACGCTTCTTGTTCCAGACACGAACAAGAAAAAGACCGAATCTTTCACCTTCCCGGTGAACCAAACGATGTCTCCTTCTCTCACTTCTCTGGTTACATTACCGTCAACGAGTCAGCAGGAAGAGCACTATTCTACTGGCTCACTGAGTCACCACCGAGTGAAAACCCTGAGTCTAAGCCTCTTGTCCTCTGGCTCAACGGTGGACCTGGTTGTTCCTCCGTAGCTTACGGTGCCGCTGAAGAAATCGGACCTTTTAGAATCAATCCTGATGGCAAAACTCTTTACCACAATCCTTACTCTTGGAACAAATTGGCGAATTTGCTCTTCCTTGAATCTCCTGCTGGTGTTGGTTTCTCGTATTCGAATACTACCTCCGATTTGTATACTGCCGGAGATCAGAGAACTGCGGAAGATGCTTATGTGTTTCTTGTGAAATGGTTTGAGAGGTTTCCTCAATACAAACACAGAGAGTTCTACATTGCTGGAGAAAGCTATGCAGGTCATTATGTTCCTCAGTTGTCACAGATTGTTTATGAGAAACGCAATCCAGCTATCAACTTTAAAGGCTTCATTGTTGGGAATGCTGTGATTGATGACTACCATGATTACGTGGGTTTATTTGAATATTGGTGGGCTCATGGGTTGATATCTGATCTCACTTACCACAACTTACGGATCACGTGTGAATTTGGATCATCCGAGCACCCGTCCTCTAAATGCACCAAGGCCATGGAAGCTGCAGACTTGGAGCAAGGCAATATTGATCCTTATAGCATTTACACTGTCACTTGTAAAAAGGAGGCTGCAGCTCTTAGGTCTCGCTTCTCGAGAGTTCGTCATCCATGGATGTGGAGAGCCTATGACCCTTGCACAGAGAAATACTCCGGCATGTATTTCAATTCTCCGGAGGTTCAAAAGGCTATGCATGCTAATATAACAGGACTAGCTTATCCATGGAAAGGGTGCAGTGACATCGTTGGAGAGAAATGGGCAGATTCTCCTCTGTCTATGCTTCCAATCTACAAAGAACTCATCGCCGCAGGTCTCAGGATATGGGTTTTCAGCGGAGACACTGATTCAGTGGTTCCCATTACTGGAACACGATACTCCATTAGAGCCCTCAAGTTACAACCACTCTCCAAATGGTACCCTTGGAACGATGATGGACAGGTTGGTGGATGGAGCCAAGTTTACAAAGGGCTGACTCTGGTGACAATACATGGAGCAGGACATGAGGTACCTCTTTTCCGCCCTCGTCGAGCTTTTCTTCTTTTTCAGTCGTTTCTCGACAACAAGCCATTGCCAATGTAA

Protein Analysis

452

Amino Acids

51.52

Weight (kDa)

6.43

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 34 - 447 2.2e-147 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 536, 1079
Acc65I GGTACC 2 cut(s) 1194, 1285
AccB1I GGYRCC 3 cut(s) 278, 1194, 1285
AccI GTMKAC 1 cut(s) 431
AccIII TCCGGA 1 cut(s) 952
AciI CCGC 5 cut(s) 282, 455, 1086, 1113, 1297
AclWI GGATC 3 cut(s) 731, 748, 806
AcsI RAATTY 2 cut(s) 364, 734
AcuI CTGAAG 1 cut(s) 306
AcvI CACGTG 1 cut(s) 729
AdeI CACNNNGTG 1 cut(s) 206
AfaI GTAC 2 cut(s) 1196, 1287
AfiI CCNNNNNNNGG 2 cut(s) 1007, 1142
AflIII ACRYGT 1 cut(s) 728
AgsI TTSAA 4 cut(s) 380, 671, 946, 962
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 7 cut(s) 272, 540, 599, 785, 857, 995, 1310
AluI AGCT 7 cut(s) 272, 540, 599, 785, 857, 995, 1310
Alw21I GWGCWC 2 cut(s) 175, 753
Alw26I GTCTC 4 cut(s) 124, 870, 1097, 1110
AlwI GGATC 3 cut(s) 731, 748, 806
Ama87I CYCGRG 1 cut(s) 874
Aor13HI TCCGGA 1 cut(s) 952
AoxI GGCC 1 cut(s) 774
ApeKI GCWGC 3 cut(s) 785, 851, 854
ApoI RAATTY 2 cut(s) 364, 734
Asp718I GGTACC 2 cut(s) 1194, 1285
AspS9I GGNCC 2 cut(s) 251, 296
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 4 cut(s) 86, 116, 189, 1270
AsuII TTCGAA 1 cut(s) 410
AvaI CYCGRG 1 cut(s) 874
AvaII GGWCC 2 cut(s) 251, 296
BanI GGYRCC 3 cut(s) 278, 1194, 1285
BanII GRGCYC 2 cut(s) 685, 1168
BbrPI CACGTG 1 cut(s) 729
Bbv12I GWGCWC 2 cut(s) 175, 753
BbvI GCAGC 3 cut(s) 772, 838, 866
BccI CCATC 3 cut(s) 311, 1205, 1221
BcgI CGANNNNNNTGC 2 cut(s) 1003, 1037
BciT130I CCWGG 1 cut(s) 255
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 4 cut(s) 124, 870, 1097, 1110
BfaI CTAG 1 cut(s) 992
BfmI CTRYAG 2 cut(s) 786, 852
BfuAI ACCTGC 2 cut(s) 536, 1079
BisI GCNGC 5 cut(s) 282, 786, 852, 855, 1086
BlsI GCNGC 5 cut(s) 283, 787, 853, 856, 1087
Bme1390I CCNGG 2 cut(s) 102, 255
Bme18I GGWCC 2 cut(s) 251, 296
BmeT110I CYCGRG 1 cut(s) 874
BmgT120I GGNCC 2 cut(s) 251, 296
BmiI GGNNCC 5 cut(s) 280, 1134, 1196, 1232, 1287
BmrFI CCNGG 2 cut(s) 102, 255
BmsI GCATC 1 cut(s) 451
BplI GAGNNNNNCTC 4 cut(s) 484, 516, 914, 946
BpmI CTGGAG 1 cut(s) 552
Bpu14I TTCGAA 1 cut(s) 410
BpuEI CTTGAG 1 cut(s) 1154
BpuMI CCSGG 1 cut(s) 102
BsaAI YACGTR 2 cut(s) 658, 729
BsaI GGTCTC 2 cut(s) 870, 1097
BsaJI CCNNGG 5 cut(s) 771, 777, 890, 1001, 1199
BsaWI WCCGGW 1 cut(s) 952
BsaXI ACNNNNNCTCC 3 cut(s) 30, 104, 134
Bsc4I CCNNNNNNNGG 2 cut(s) 1007, 1142
Bse1I ACTGG 2 cut(s) 188, 1147
Bse3DI GCAATG 2 cut(s) 525, 1346
BseAI TCCGGA 1 cut(s) 952
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 5 cut(s) 771, 777, 890, 1001, 1199
BseGI GGATG 4 cut(s) 743, 886, 900, 1232
BseLI CCNNNNNNNGG 2 cut(s) 1007, 1142
BseMI GCAATG 2 cut(s) 525, 1346
BseMII CTCAG 4 cut(s) 183, 207, 575, 1108
BseNI ACTGG 2 cut(s) 188, 1147
BseRI GAGGAG 2 cut(s) 8, 1038
BseXI GCAGC 3 cut(s) 772, 838, 866
BsgI GTGCAG 1 cut(s) 1033
BshFI GGCC 1 cut(s) 776
BshNI GGYRCC 3 cut(s) 278, 1194, 1285
BsiHKAI GWGCWC 2 cut(s) 175, 753
BsiHKCI CYCGRG 1 cut(s) 874
BsiSI CCGG 4 cut(s) 102, 438, 933, 953
BslI CCNNNNNNNGG 2 cut(s) 1007, 1142
BsmAI GTCTC 4 cut(s) 124, 870, 1097, 1110
BsmI GAATGC 1 cut(s) 634
BsnI GGCC 1 cut(s) 776
Bso31I GGTCTC 2 cut(s) 870, 1097
BsoBI CYCGRG 1 cut(s) 874
Bsp119I TTCGAA 1 cut(s) 410
Bsp1286I GDGCHC 4 cut(s) 175, 685, 753, 1168
Bsp13I TCCGGA 1 cut(s) 952
Bsp143I GATC 5 cut(s) 442, 700, 723, 740, 811
Bsp19I CCATGG 3 cut(s) 777, 890, 1001
BspACI CCGC 5 cut(s) 282, 455, 1086, 1113, 1297
BspANI GGCC 1 cut(s) 776
BspCNI CTCAG 4 cut(s) 184, 208, 574, 1107
BspEI TCCGGA 1 cut(s) 952
BspLI GGNNCC 5 cut(s) 280, 1134, 1196, 1232, 1287
BspMAI CTGCAG 2 cut(s) 790, 856
BspMI ACCTGC 2 cut(s) 536, 1079
BspPI GGATC 3 cut(s) 731, 748, 806
BspQI GCTCTTC 2 cut(s) 163, 377
BspT104I TTCGAA 1 cut(s) 410
BspT107I GGYRCC 3 cut(s) 278, 1194, 1285
BspTNI GGTCTC 2 cut(s) 870, 1097
BsrDI GCAATG 2 cut(s) 525, 1346
BsrI ACTGG 2 cut(s) 188, 1147
BssECI CCNNGG 5 cut(s) 771, 777, 890, 1001, 1199
BssMI GATC 5 cut(s) 442, 700, 723, 740, 811
BssNAI GTATAC 1 cut(s) 432
BssT1I CCWWGG 5 cut(s) 771, 777, 890, 1001, 1199
Bst1107I GTATAC 1 cut(s) 432
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 4 cut(s) 151, 248, 278, 832
Bst6I CTCTTC 3 cut(s) 26, 163, 377
BstBAI YACGTR 2 cut(s) 658, 729
BstBI TTCGAA 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 975
BstDEI CTNAG 6 cut(s) 192, 216, 222, 561, 860, 1094
BstDSI CCRYGG 3 cut(s) 777, 890, 1001
BstF5I GGATG 4 cut(s) 743, 886, 900, 1232
BstKTI GATC 5 cut(s) 445, 703, 726, 743, 814
BstMAI GTCTC 4 cut(s) 124, 870, 1097, 1110
BstMBI GATC 5 cut(s) 442, 700, 723, 740, 811
BstMWI GCNNNNNNNGC 5 cut(s) 170, 278, 461, 782, 974
BstNI CCWGG 1 cut(s) 255
BstNSI RCATGY 2 cut(s) 940, 977
BstSCI CCNGG 2 cut(s) 100, 253
BstSFI CTRYAG 2 cut(s) 786, 852
BstV1I GCAGC 3 cut(s) 772, 838, 866
BstXI CCANNNNNNTGG 2 cut(s) 778, 897
BstZ17I GTATAC 1 cut(s) 432
BsuRI GGCC 1 cut(s) 776
BtgI CCRYGG 3 cut(s) 777, 890, 1001
BtgZI GCGATG 1 cut(s) 1066
BtsCI GGATG 4 cut(s) 743, 886, 900, 1232
BtsI GCAGTG 1 cut(s) 1021
BtsIMutI CAGTG 5 cut(s) 189, 828, 1021, 1119, 1134
BveI ACCTGC 2 cut(s) 536, 1079
Cac8I GCNNGC 1 cut(s) 975
Cfr13I GGNCC 2 cut(s) 251, 296
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 2 cut(s) 1195, 1286
CviAII CATG 9 cut(s) 650, 686, 778, 891, 937, 974, 1002, 1268, 1280
CviQI GTAC 2 cut(s) 1195, 1286
DdeI CTNAG 6 cut(s) 192, 216, 222, 561, 860, 1094
DpnI GATC 5 cut(s) 444, 702, 725, 742, 813
DpnII GATC 5 cut(s) 442, 700, 723, 740, 811
DraI TTTAAA 1 cut(s) 610
DraIII CACNNNGTG 1 cut(s) 206
Eam1104I CTCTTC 3 cut(s) 26, 163, 377
EarI CTCTTC 3 cut(s) 26, 163, 377
EciI GGCGGA 1 cut(s) 1286
Eco130I CCWWGG 5 cut(s) 771, 777, 890, 1001, 1199
Eco24I GRGCYC 2 cut(s) 685, 1168
Eco31I GGTCTC 2 cut(s) 870, 1097
Eco32I GATATC 1 cut(s) 696
Eco47I GGWCC 2 cut(s) 251, 296
Eco57I CTGAAG 1 cut(s) 306
Eco72I CACGTG 1 cut(s) 729
Eco88I CYCGRG 1 cut(s) 874
EcoRII CCWGG 1 cut(s) 253
EcoRV GATATC 1 cut(s) 696
EcoT14I CCWWGG 5 cut(s) 771, 777, 890, 1001, 1199
EcoT22I ATGCAT 1 cut(s) 975
EcoT38I GRGCYC 2 cut(s) 685, 1168
ErhI CCWWGG 5 cut(s) 771, 777, 890, 1001, 1199
FaeI CATG 9 cut(s) 653, 689, 781, 894, 940, 977, 1005, 1271, 1283
FatI CATG 9 cut(s) 649, 685, 777, 890, 936, 973, 1001, 1267, 1279
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 5 cut(s) 282, 786, 852, 855, 1086
FokI GGATG 4 cut(s) 730, 873, 907, 1239
FriOI GRGCYC 2 cut(s) 685, 1168
Fsp4HI GCNGC 5 cut(s) 282, 786, 852, 855, 1086
FspBI CTAG 1 cut(s) 992
GluI GCNGC 5 cut(s) 282, 786, 852, 855, 1086
GsuI CTGGAG 1 cut(s) 552
HaeIII GGCC 1 cut(s) 776
HapII CCGG 4 cut(s) 102, 438, 933, 953
Hin1II CATG 9 cut(s) 653, 689, 781, 894, 940, 977, 1005, 1271, 1283
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HpaII CCGG 4 cut(s) 102, 438, 933, 953
HphI GGTGA 4 cut(s) 86, 116, 189, 1270
Hpy166II GTNNAC 5 cut(s) 107, 154, 251, 432, 1240
Hpy188I TCNGA 5 cut(s) 296, 424, 447, 700, 748
Hpy188III TCNNGA 7 cut(s) 63, 314, 874, 876, 953, 1096, 1334
Hpy8I GTNNAC 5 cut(s) 107, 154, 251, 432, 1240
Hpy99I CGWCG 1 cut(s) 1308
HpyAV CCTTC 3 cut(s) 23, 107, 133
HpyCH4III ACNGT 4 cut(s) 151, 248, 278, 832
HpyCH4IV ACGT 2 cut(s) 657, 728
HpyCH4V TGCA 7 cut(s) 545, 768, 788, 854, 918, 973, 1014
HpyF10VI GCNNNNNNNGC 5 cut(s) 170, 278, 461, 782, 974
HpyF3I CTNAG 6 cut(s) 192, 216, 222, 561, 860, 1094
HpySE526I ACGT 2 cut(s) 657, 728
Hsp92II CATG 9 cut(s) 653, 689, 781, 894, 940, 977, 1005, 1271, 1283
Kpn2I TCCGGA 1 cut(s) 952
KpnI GGTACC 2 cut(s) 1198, 1289
Kzo9I GATC 5 cut(s) 442, 700, 723, 740, 811
LguI GCTCTTC 2 cut(s) 163, 377
LmnI GCTCC 3 cut(s) 796, 1230, 1271
Lsp1109I GCAGC 3 cut(s) 772, 838, 866
LweI GCATC 1 cut(s) 451
MabI ACCWGGT 1 cut(s) 253
MaeI CTAG 1 cut(s) 992
MaeII ACGT 2 cut(s) 657, 728
MaeIII GTNAC 7 cut(s) 140, 195, 567, 832, 1016, 1173, 1258
MalI GATC 5 cut(s) 444, 702, 725, 742, 813
MboI GATC 5 cut(s) 442, 700, 723, 740, 811
MboII GAAGA 9 cut(s) 13, 16, 19, 22, 180, 299, 364, 470, 1307
MhlI GDGCHC 4 cut(s) 175, 685, 753, 1168
MluCI AATT 4 cut(s) 356, 364, 734, 946
MlyI GAGTC 5 cut(s) 3, 167, 203, 227, 1246
MmeI TCCRAC 1 cut(s) 1006
Mph1103I ATGCAT 1 cut(s) 975
MroI TCCGGA 1 cut(s) 952
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 895
MspA1I CMGCKG 2 cut(s) 284, 1113
MspI CCGG 4 cut(s) 102, 438, 933, 953
MspR9I CCNGG 2 cut(s) 102, 255
Mva1269I GAATGC 1 cut(s) 634
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 5 cut(s) 170, 278, 461, 782, 974
NciI CCSGG 1 cut(s) 102
NcoI CCATGG 3 cut(s) 777, 890, 1001
NdeII GATC 5 cut(s) 442, 700, 723, 740, 811
NlaIII CATG 9 cut(s) 653, 689, 781, 894, 940, 977, 1005, 1271, 1283
NlaIV GGNNCC 5 cut(s) 280, 1134, 1196, 1232, 1287
NmuCI GTSAC 5 cut(s) 195, 567, 832, 1016, 1258
NsiI ATGCAT 1 cut(s) 975
NspI RCATGY 2 cut(s) 940, 977
NspV TTCGAA 1 cut(s) 410
PaeI GCATGC 1 cut(s) 977
PaeR7I CTCGAG 1 cut(s) 874
PciSI GCTCTTC 2 cut(s) 163, 377
PctI GAATGC 1 cut(s) 634
PfeI GAWTC 5 cut(s) 87, 306, 380, 1043, 1124
PkrI GCNGC 5 cut(s) 283, 787, 853, 856, 1087
PleI GAGTC 5 cut(s) 3, 166, 202, 226, 1246
PmaCI CACGTG 1 cut(s) 729
PmlI CACGTG 1 cut(s) 729
PpsI GAGTC 5 cut(s) 3, 166, 202, 226, 1246
Ppu21I YACGTR 2 cut(s) 658, 729
Psp6I CCWGG 1 cut(s) 253
PspCI CACGTG 1 cut(s) 729
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 5 cut(s) 280, 1134, 1196, 1232, 1287
PspPI GGNCC 2 cut(s) 251, 296
PstI CTGCAG 2 cut(s) 790, 856
RsaI GTAC 2 cut(s) 1196, 1287
RsaNI GTAC 2 cut(s) 1195, 1286
RseI CAYNNNNRTG 1 cut(s) 895
SapI GCTCTTC 2 cut(s) 163, 377
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 5 cut(s) 282, 786, 852, 855, 1086
Sau3AI GATC 5 cut(s) 442, 700, 723, 740, 811
Sau96I GGNCC 2 cut(s) 251, 296
SchI GAGTC 5 cut(s) 3, 167, 203, 227, 1246
ScrFI CCNGG 2 cut(s) 102, 255
SduI GDGCHC 4 cut(s) 175, 685, 753, 1168
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 1 cut(s) 451
SfcI CTRYAG 2 cut(s) 786, 852
Sfr274I CTCGAG 1 cut(s) 874
SfuI TTCGAA 1 cut(s) 410
SinI GGWCC 2 cut(s) 251, 296
SlaI CTCGAG 1 cut(s) 874
SmiMI CAYNNNNRTG 1 cut(s) 895
SmlI CTYRAG 2 cut(s) 874, 1169
SmoI CTYRAG 2 cut(s) 874, 1169
SphI GCATGC 1 cut(s) 977
Sse9I AATT 4 cut(s) 356, 364, 734, 946
SsiI CCGC 5 cut(s) 282, 455, 1086, 1113, 1297
SspI AATATT 2 cut(s) 674, 808
SspMI CTAG 1 cut(s) 992
StyD4I CCNGG 2 cut(s) 100, 253
StyI CCWWGG 5 cut(s) 771, 777, 890, 1001, 1199
TaaI ACNGT 4 cut(s) 151, 248, 278, 832
TaiI ACGT 2 cut(s) 660, 731
TaqI TCGA 5 cut(s) 7, 410, 875, 1306, 1335
TaqII GACCGA 1 cut(s) 99
TasI AATT 4 cut(s) 356, 364, 734, 946
TauI GCSGC 2 cut(s) 284, 1088
TfiI GAWTC 5 cut(s) 87, 306, 380, 1043, 1124
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 5 cut(s) 196, 835, 1021, 1126, 1134
TseFI GTSAC 5 cut(s) 195, 567, 832, 1016, 1258
TseI GCWGC 3 cut(s) 785, 851, 854
Tsp45I GTSAC 5 cut(s) 195, 567, 832, 1016, 1258
TspDTI ATGAA 1 cut(s) 607
TspGWI ACGGA 2 cut(s) 256, 736
TspRI CASTG 5 cut(s) 196, 835, 1021, 1126, 1134
VpaK11BI GGWCC 2 cut(s) 251, 296
XapI RAATTY 2 cut(s) 364, 734
XceI RCATGY 2 cut(s) 940, 977
XcmI CCANNNNNNNNNTGG 1 cut(s) 1188
XhoI CTCGAG 1 cut(s) 874
XmiI GTMKAC 1 cut(s) 431
XspI CTAG 1 cut(s) 992
Zsp2I ATGCAT 1 cut(s) 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.