Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0004331

Physical Location & Seq

Genomic Coordinates Reverse (-)

25582822 .. 25587931

Gene Structure 5110 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1176500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1368 bp

ATGGGTGGCCACTCTCCATTTGCTTTTCCTTGCATTTTGCTTCTTTTCTTGGGGGGTTGTTTTGCTTCTGTTCAAGATCAAGTGAGGGATAGAATCACACAATTGCCAGGACAGCCAACTGTGGGGTTTGCTCAGTACTCTGGTTATGTAACTGTCAATAGGAAAGCCGGTAGAGCATTGTTTTACTGGTTGACTGAATCCCCTGCAAATCGTCGGCCCGAGTCTAGACCACTAATGTTGTGGCTTAATGGTGGCCCTGGTTGCTCTTCTGTTGCTTATGGAGCAGCTGAAGAGATTGGACCATTTCATATTAGACCTGATGGAAAGACCCTTTACTTGAATCCCTATGCTTGGAACAACTTGGCAAATTTGCTATTCCTTGAATCACCAGCTGGTGTCGGCTTTTCATATACGAATACGAGCTCAGATTTGTATACAGCCGGAGACCAGAGAACAGCTGAAGATGCGTATGCATTTCTAGTCAATTGGTTCGAAAGGTTCCCACAGTACAAGCACAGAGATTTCTACATTGCTGGAGAAAGTTATGCAGGTCATTATGTTCCTCAGTTGTCTCAAATCATTTATGAGAGAAATAAGGGAAAGAAGAACCCAATTATTAATTTCAAGGGATTTATGGTGGGAAATGCTGTGACTGATGATTATCATGATTATGTTGGCACATTTGAATACTGGTGGAACCATGGTTTAATTTCTGATTCTACCTATCGATCGCTACGTGTAACCTGTGACTCTGGATCCTCTCAGCATCCGTCAGTGGACTGCATGAGGGCGCTTGAACTAGCAGAAATGGAGCAGGGAAACATCGATCCATACAGCATTTATACTCGTCCTTGCAATAGCACTGCATCCCTAAAGCGTAATTTGAGGGGTCATTATCCATGGATGTCCAGAGCATATGATCCCTGCACTGAGAGGTATTCTGAAGTTTATTTCAACCGTCCAGACGTTCAAAAGGCACTCCATGCCAATGTAACTAAAGTTTCTTATCCATGGCAAACATGCAGTGATATTGTTGGAAACTACTGGGCCGATTCTCCTCTGTCCATGCTTCCGATTTACAAGGAGCTCATAGCTGCTGGTCTTAAGATATGGGTATTCAGTGGAGACACTGATTCAGTGGTTCCTGTGACTGCTACTCGTTACTCCATAGATGCTCTGAAGCTACCAACCATTACCAACTGGTTTCCATGGAACGACAATGGCAAGGTTGGCGGCCGGAGCCAAATATACAAGGGTCTGACATTTGTCACTGTGACCGGAGCTGGGCACGAGGTTCCACTCCATCGACCTCGCCTAGCTTTCATTCTTTTCCGATCATTCTTGGAGAGCAAGCCAATGCCAAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004180 GO:0004185 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0017171 GO:0019538 GO:0030163 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0070008 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S10

Protein Analysis

456

Amino Acids

51.37

Weight (kDa)

8.01

Isoelectric Point (pI)

42.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S10	PF00450	36 - 449	5.8e-148	Serine carboxypeptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004331)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35780 AT3G07990
fragaria_vesca	FvH4_7g19810 FvH4_7g19810 FvH4_7g19810 FvH4_7g19810 FvH4_7g19810 FvH4_7g19810 FvH4_7g19840 FvH4_7g19840 FvH4_7g19840 FvH4_7g19840
malus_domestica	MD01G1110700.v1.1 MD07G1176500.v1.1
prunus_persica	Prupe.2G215500_v2.0.a1 Prupe.2G215500_v2.0.a1
pyrus_communis	pycom01g13780 pycom07g17070
rosa_chinensis	RchiOBHm_Chr1g0363811 RchiOBHm_Chr1g0363831
rosa_laevigata	RLG00000027543 RLG00000027544
rosa_multiflora	Rmu_sc0002258.1_g000001
rosa_roxburghii	Rroxscaffold_4G00292950
rosa_rugosa	Rorug01G0309200
rosa_samantha	Rh1AG318000 Rh1AG318100 Rh1BG281300 Rh1CG297600 Rh1CG297700
rosa_wichuraiana	Rw1G028220 Rw1G028230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	539
AccI	GTMKAC	1 cut(s)	434
AciI	CCGC	1 cut(s)	1233
AclWI	GGATC	4 cut(s)	750, 763, 821, 914
AcoI	YGGCCR	2 cut(s)	7, 1234
AcsI	RAATTY	1 cut(s)	367
AcuI	CTGAAG	4 cut(s)	309, 480, 963, 1199
AfaI	GTAC	2 cut(s)	137, 509
AfiI	CCNNNNNNNGG	3 cut(s)	122, 351, 1284
AflII	CTTAAG	1 cut(s)	1103
AflIII	ACRYGT	1 cut(s)	736
AgsI	TTSAA	8 cut(s)	74, 340, 383, 625, 686, 797, 955, 971
AjnI	CCWGG	2 cut(s)	106, 256
AluBI	AGCT	9 cut(s)	287, 392, 423, 458, 1087, 1094, 1183, 1283, 1319
AluI	AGCT	9 cut(s)	287, 392, 423, 458, 1087, 1094, 1183, 1283, 1319
Alw21I	GWGCWC	2 cut(s)	425, 1089
Alw26I	GTCTC	3 cut(s)	438, 576, 1119
AlwI	GGATC	4 cut(s)	750, 763, 821, 914
Ama87I	CYCGRG	1 cut(s)	218
AoxI	GGCC	5 cut(s)	7, 215, 253, 1047, 1234
ApeKI	GCWGC	2 cut(s)	284, 1094
ApoI	RAATTY	1 cut(s)	367
AseI	ATTAAT	1 cut(s)	618
AspLEI	GCGC	1 cut(s)	793
AspS9I	GGNCC	4 cut(s)	216, 254, 299, 1047
AsuHPI	GGTGA	1 cut(s)	378
AsuII	TTCGAA	1 cut(s)	492
AvaI	CYCGRG	1 cut(s)	218
AvaII	GGWCC	1 cut(s)	299
BaeGI	GKGCMC	1 cut(s)	1290
BalI	TGGCCA	1 cut(s)	9
BamHI	GGATCC	1 cut(s)	755
BanII	GRGCYC	2 cut(s)	425, 1089
BauI	CACGAG	1 cut(s)	1289
Bbv12I	GWGCWC	2 cut(s)	425, 1089
BbvI	GCAGC	2 cut(s)	296, 1081
BccI	CCATC	2 cut(s)	314, 1311
BciT130I	CCWGG	2 cut(s)	108, 258
BcoDI	GTCTC	3 cut(s)	438, 576, 1119
BfaI	CTAG	4 cut(s)	225, 479, 800, 1316
BfoI	RGCGCY	1 cut(s)	794
BfrI	CTTAAG	1 cut(s)	1103
BfuAI	ACCTGC	1 cut(s)	539
BisI	GCNGC	3 cut(s)	285, 1095, 1234
BlsI	GCNGC	3 cut(s)	286, 1096, 1235
BmcAI	AGTACT	1 cut(s)	137
Bme1390I	CCNGG	2 cut(s)	108, 258
Bme18I	GGWCC	1 cut(s)	299
BmeT110I	CYCGRG	1 cut(s)	218
BmgT120I	GGNCC	4 cut(s)	216, 254, 299, 1047
BmiI	GGNNCC	6 cut(s)	500, 698, 757, 1143, 1241, 1296
BmrFI	CCNGG	2 cut(s)	108, 258
BmrI	ACTGGG	1 cut(s)	1054
BmsI	GCATC	4 cut(s)	454, 775, 875, 1162
BmuI	ACTGGG	1 cut(s)	1054
BoxI	GACNNNNGTC	1 cut(s)	1265
BpmI	CTGGAG	1 cut(s)	555
Bpu14I	TTCGAA	1 cut(s)	492
Bsa29I	ATCGAT	2 cut(s)	727, 825
BsaAI	YACGTR	1 cut(s)	737
BsaBI	GATNNNNATC	1 cut(s)	660
BsaI	GGTCTC	1 cut(s)	438
BsaJI	CCNNGG	5 cut(s)	256, 700, 899, 1010, 1208
BsaWI	WCCGGW	1 cut(s)	1277
Bsc4I	CCNNNNNNNGG	3 cut(s)	122, 351, 1284
Bse118I	RCCGGY	1 cut(s)	167
Bse1I	ACTGG	4 cut(s)	191, 695, 1049, 1205
Bse3DI	GCAATG	1 cut(s)	528
Bse8I	GATNNNNATC	1 cut(s)	660
BseBI	CCWGG	2 cut(s)	108, 258
BseCI	ATCGAT	2 cut(s)	727, 825
BseDI	CCNNGG	5 cut(s)	256, 700, 899, 1010, 1208
BseGI	GGATG	3 cut(s)	766, 866, 909
BseJI	GATNNNNATC	1 cut(s)	660
BseLI	CCNNNNNNNGG	3 cut(s)	122, 351, 1284
BseMI	GCAATG	1 cut(s)	528
BseMII	CTCAG	5 cut(s)	146, 438, 578, 776, 921
BseNI	ACTGG	4 cut(s)	191, 695, 1049, 1205
BseRI	GAGGAG	1 cut(s)	1047
BseSI	GKGCMC	1 cut(s)	1290
BseX3I	CGGCCG	1 cut(s)	1234
BseXI	GCAGC	2 cut(s)	296, 1081
BseYI	CCCAGC	1 cut(s)	1283
BsgI	GTGCAG	1 cut(s)	910
Bsh1285I	CGRYCG	2 cut(s)	731, 1237
BshFI	GGCC	5 cut(s)	9, 217, 255, 1049, 1236
BshVI	ATCGAT	2 cut(s)	727, 825
BsiEI	CGRYCG	2 cut(s)	731, 1237
BsiHKAI	GWGCWC	2 cut(s)	425, 1089
BsiHKCI	CYCGRG	1 cut(s)	218
BsiSI	CCGG	4 cut(s)	168, 441, 1237, 1278
BslI	CCNNNNNNNGG	3 cut(s)	122, 351, 1284
BsmAI	GTCTC	3 cut(s)	438, 576, 1119
BsnI	GGCC	5 cut(s)	9, 217, 255, 1049, 1236
Bso31I	GGTCTC	1 cut(s)	438
BsoBI	CYCGRG	1 cut(s)	218
Bsp119I	TTCGAA	1 cut(s)	492
Bsp1286I	GDGCHC	3 cut(s)	425, 1089, 1290
Bsp143I	GATC	6 cut(s)	76, 728, 755, 826, 919, 1334
Bsp19I	CCATGG	4 cut(s)	700, 899, 1010, 1208
BspACI	CCGC	1 cut(s)	1233
BspANI	GGCC	5 cut(s)	9, 217, 255, 1049, 1236
BspCNI	CTCAG	5 cut(s)	145, 437, 577, 775, 922
BspDI	ATCGAT	2 cut(s)	727, 825
BspHI	TCATGA	1 cut(s)	664
BspLI	GGNNCC	6 cut(s)	500, 698, 757, 1143, 1241, 1296
BspMI	ACCTGC	1 cut(s)	539
BspPI	GGATC	4 cut(s)	750, 763, 821, 914
BspQI	GCTCTTC	1 cut(s)	271
BspT104I	TTCGAA	1 cut(s)	492
BspTI	CTTAAG	1 cut(s)	1103
BspTNI	GGTCTC	1 cut(s)	438
BsrDI	GCAATG	1 cut(s)	528
BsrFI	RCCGGY	1 cut(s)	167
BsrI	ACTGG	4 cut(s)	191, 695, 1049, 1205
BssAI	RCCGGY	1 cut(s)	167
BssECI	CCNNGG	5 cut(s)	256, 700, 899, 1010, 1208
BssMI	GATC	6 cut(s)	76, 728, 755, 826, 919, 1334
BssNAI	GTATAC	1 cut(s)	435
BssSI	CACGAG	1 cut(s)	1289
BssT1I	CCWWGG	4 cut(s)	700, 899, 1010, 1208
Bst1107I	GTATAC	1 cut(s)	435
Bst2BI	CACGAG	1 cut(s)	1289
Bst2UI	CCWGG	2 cut(s)	108, 258
Bst4CI	ACNGT	5 cut(s)	121, 154, 507, 959, 1273
Bst6I	CTCTTC	2 cut(s)	271, 285
BstAFI	CTTAAG	1 cut(s)	1103
BstAPI	GCANNNNNTGC	1 cut(s)	983
BstBAI	YACGTR	1 cut(s)	737
BstBI	TTCGAA	1 cut(s)	492
BstC8I	GCNNGC	1 cut(s)	1352
BstDEI	CTNAG	5 cut(s)	132, 424, 564, 762, 930
BstDSI	CCRYGG	4 cut(s)	700, 899, 1010, 1208
BstF5I	GGATG	3 cut(s)	766, 866, 909
BstH2I	RGCGCY	1 cut(s)	794
BstHHI	GCGC	1 cut(s)	793
BstKTI	GATC	6 cut(s)	79, 731, 758, 829, 922, 1337
BstMAI	GTCTC	3 cut(s)	438, 576, 1119
BstMBI	GATC	6 cut(s)	76, 728, 755, 826, 919, 1334
BstMCI	CGRYCG	2 cut(s)	731, 1237
BstMWI	GCNNNNNNNGC	8 cut(s)	112, 173, 261, 281, 464, 983, 1230, 1239
BstNI	CCWGG	2 cut(s)	108, 258
BstNSI	RCATGY	1 cut(s)	1023
BstPAI	GACNNNNGTC	1 cut(s)	1265
BstSCI	CCNGG	2 cut(s)	106, 256
BstSLI	GKGCMC	1 cut(s)	1290
BstV1I	GCAGC	2 cut(s)	296, 1081
BstX2I	RGATCY	1 cut(s)	755
BstYI	RGATCY	1 cut(s)	755
BstZ17I	GTATAC	1 cut(s)	435
BstZI	CGGCCG	1 cut(s)	1234
Bsu15I	ATCGAT	2 cut(s)	727, 825
BsuRI	GGCC	5 cut(s)	9, 217, 255, 1049, 1236
BsuTUI	ATCGAT	2 cut(s)	727, 825
BtgI	CCRYGG	4 cut(s)	700, 899, 1010, 1208
BtsCI	GGATG	3 cut(s)	766, 866, 909
BtsI	GCAGTG	2 cut(s)	861, 1030
BtsIMutI	CAGTG	8 cut(s)	780, 861, 927, 1030, 1126, 1128, 1143, 1269
BveI	ACCTGC	1 cut(s)	539
Cac8I	GCNNGC	1 cut(s)	1352
CciI	TCATGA	1 cut(s)	664
CfoI	GCGC	1 cut(s)	793
Cfr10I	RCCGGY	1 cut(s)	167
Cfr13I	GGNCC	4 cut(s)	216, 254, 299, 1047
ClaI	ATCGAT	2 cut(s)	727, 825
Csp6I	GTAC	2 cut(s)	136, 508
CspCI	CAANNNNNGTGG	2 cut(s)	219, 254
CviAII	CATG	9 cut(s)	665, 701, 784, 900, 983, 1011, 1020, 1066, 1209
CviQI	GTAC	2 cut(s)	136, 508
DdeI	CTNAG	5 cut(s)	132, 424, 564, 762, 930
DpnI	GATC	6 cut(s)	78, 730, 757, 828, 921, 1336
DpnII	GATC	6 cut(s)	76, 728, 755, 826, 919, 1334
EaeI	YGGCCR	2 cut(s)	7, 1234
EagI	CGGCCG	1 cut(s)	1234
Eam1104I	CTCTTC	2 cut(s)	271, 285
EarI	CTCTTC	2 cut(s)	271, 285
Ecl136II	GAGCTC	2 cut(s)	423, 1087
EclXI	CGGCCG	1 cut(s)	1234
Eco130I	CCWWGG	4 cut(s)	700, 899, 1010, 1208
Eco24I	GRGCYC	2 cut(s)	425, 1089
Eco31I	GGTCTC	1 cut(s)	438
Eco47I	GGWCC	1 cut(s)	299
Eco52I	CGGCCG	1 cut(s)	1234
Eco53kI	GAGCTC	2 cut(s)	423, 1087
Eco57I	CTGAAG	4 cut(s)	309, 480, 963, 1199
Eco88I	CYCGRG	1 cut(s)	218
EcoICRI	GAGCTC	2 cut(s)	423, 1087
EcoRII	CCWGG	2 cut(s)	106, 256
EcoT14I	CCWWGG	4 cut(s)	700, 899, 1010, 1208
EcoT22I	ATGCAT	1 cut(s)	475
EcoT38I	GRGCYC	2 cut(s)	425, 1089
ErhI	CCWWGG	4 cut(s)	700, 899, 1010, 1208
FaeI	CATG	9 cut(s)	668, 704, 787, 903, 986, 1014, 1023, 1069, 1212
FatI	CATG	9 cut(s)	664, 700, 783, 899, 982, 1010, 1019, 1065, 1208
FauNDI	CATATG	1 cut(s)	916
FblI	GTMKAC	1 cut(s)	434
Fnu4HI	GCNGC	3 cut(s)	285, 1095, 1234
FokI	GGATG	3 cut(s)	753, 853, 916
FriOI	GRGCYC	2 cut(s)	425, 1089
Fsp4HI	GCNGC	3 cut(s)	285, 1095, 1234
FspBI	CTAG	4 cut(s)	225, 479, 800, 1316
GlaI	GCGC	1 cut(s)	792
GluI	GCNGC	3 cut(s)	285, 1095, 1234
GsaI	CCCAGC	1 cut(s)	1287
GsuI	CTGGAG	1 cut(s)	555
HaeII	RGCGCY	1 cut(s)	794
HaeIII	GGCC	5 cut(s)	9, 217, 255, 1049, 1236
HapII	CCGG	4 cut(s)	168, 441, 1237, 1278
HhaI	GCGC	1 cut(s)	793
Hin1II	CATG	9 cut(s)	668, 704, 787, 903, 986, 1014, 1023, 1069, 1212
Hin6I	GCGC	1 cut(s)	791
HinP1I	GCGC	1 cut(s)	791
HincII	GTYRAC	1 cut(s)	192
HindII	GTYRAC	1 cut(s)	192
HinfI	GANTC	9 cut(s)	93, 197, 221, 340, 383, 716, 749, 1052, 1133
HpaII	CCGG	4 cut(s)	168, 441, 1237, 1278
HphI	GGTGA	1 cut(s)	378
Hpy166II	GTNNAC	3 cut(s)	192, 435, 778
Hpy188I	TCNGA	7 cut(s)	427, 715, 943, 1074, 1179, 1260, 1334
Hpy188III	TCNNGA	6 cut(s)	74, 225, 665, 753, 909, 962
Hpy8I	GTNNAC	3 cut(s)	192, 435, 778
Hpy99I	CGWCG	1 cut(s)	216
HpyCH4III	ACNGT	5 cut(s)	121, 154, 507, 959, 1273
HpyCH4IV	ACGT	2 cut(s)	736, 966
HpyCH4V	TGCA	9 cut(s)	33, 206, 473, 548, 783, 855, 866, 927, 1023
HpyF10VI	GCNNNNNNNGC	8 cut(s)	112, 173, 261, 281, 464, 983, 1230, 1239
HpyF3I	CTNAG	5 cut(s)	132, 424, 564, 762, 930
HpySE526I	ACGT	2 cut(s)	736, 966
Hsp92II	CATG	9 cut(s)	668, 704, 787, 903, 986, 1014, 1023, 1069, 1212
HspAI	GCGC	1 cut(s)	791
Kzo9I	GATC	6 cut(s)	76, 728, 755, 826, 919, 1334
LguI	GCTCTTC	1 cut(s)	271
LmnI	GCTCC	5 cut(s)	281, 811, 1084, 1239, 1280
Lsp1109I	GCAGC	2 cut(s)	296, 1081
LweI	GCATC	4 cut(s)	454, 775, 875, 1162
MaeI	CTAG	4 cut(s)	225, 479, 800, 1316
MaeII	ACGT	2 cut(s)	736, 966
MaeIII	GTNAC	9 cut(s)	148, 649, 739, 746, 991, 1147, 1160, 1267, 1273
MalI	GATC	6 cut(s)	78, 730, 757, 828, 921, 1336
MboI	GATC	6 cut(s)	76, 728, 755, 826, 919, 1334
MboII	GAAGA	4 cut(s)	258, 302, 473, 616
MfeI	CAATTG	2 cut(s)	101, 484
MflI	RGATCY	1 cut(s)	755
MhlI	GDGCHC	3 cut(s)	425, 1089, 1290
MlsI	TGGCCA	1 cut(s)	9
MluCI	AATT	7 cut(s)	101, 367, 484, 612, 619, 708, 880
MluNI	TGGCCA	1 cut(s)	9
MlyI	GAGTC	2 cut(s)	230, 743
MmeI	TCCRAC	1 cut(s)	1015
MnlI	CCTC	9 cut(s)	78, 573, 769, 780, 879, 927, 1068, 1285, 1320
Mox20I	TGGCCA	1 cut(s)	9
Mph1103I	ATGCAT	1 cut(s)	475
MscI	TGGCCA	1 cut(s)	9
MseI	TTAA	4 cut(s)	246, 618, 707, 1104
MslI	CAYNNNNRTG	2 cut(s)	669, 987
Msp20I	TGGCCA	1 cut(s)	9
MspA1I	CMGCKG	3 cut(s)	287, 392, 458
MspCI	CTTAAG	1 cut(s)	1103
MspI	CCGG	4 cut(s)	168, 441, 1237, 1278
MspR9I	CCNGG	2 cut(s)	108, 258
MunI	CAATTG	2 cut(s)	101, 484
MvaI	CCWGG	2 cut(s)	108, 258
MwoI	GCNNNNNNNGC	8 cut(s)	112, 173, 261, 281, 464, 983, 1230, 1239
NcoI	CCATGG	4 cut(s)	700, 899, 1010, 1208
NdeI	CATATG	1 cut(s)	916
NdeII	GATC	6 cut(s)	76, 728, 755, 826, 919, 1334
NlaIII	CATG	9 cut(s)	668, 704, 787, 903, 986, 1014, 1023, 1069, 1212
NlaIV	GGNNCC	6 cut(s)	500, 698, 757, 1143, 1241, 1296
NmuCI	GTSAC	5 cut(s)	649, 746, 1147, 1267, 1273
NsiI	ATGCAT	1 cut(s)	475
NspI	RCATGY	1 cut(s)	1023
NspV	TTCGAA	1 cut(s)	492
PagI	TCATGA	1 cut(s)	664
PciSI	GCTCTTC	1 cut(s)	271
PcsI	WCGNNNNNNNCGW	1 cut(s)	733
PfeI	GAWTC	7 cut(s)	93, 197, 340, 383, 716, 1052, 1133
PkrI	GCNGC	3 cut(s)	286, 1096, 1235
Ple19I	CGATCG	1 cut(s)	731
PleI	GAGTC	2 cut(s)	229, 743
PpsI	GAGTC	2 cut(s)	229, 743
Ppu21I	YACGTR	1 cut(s)	737
PshAI	GACNNNNGTC	1 cut(s)	1265
PshBI	ATTAAT	1 cut(s)	618
Psp124BI	GAGCTC	2 cut(s)	425, 1089
Psp6I	CCWGG	2 cut(s)	106, 256
PspFI	CCCAGC	1 cut(s)	1283
PspGI	CCWGG	2 cut(s)	106, 256
PspN4I	GGNNCC	6 cut(s)	500, 698, 757, 1143, 1241, 1296
PspPI	GGNCC	4 cut(s)	216, 254, 299, 1047
PsuI	RGATCY	1 cut(s)	755
PvuI	CGATCG	1 cut(s)	731
PvuII	CAGCTG	3 cut(s)	287, 392, 458
RsaI	GTAC	2 cut(s)	137, 509
RsaNI	GTAC	2 cut(s)	136, 508
RseI	CAYNNNNRTG	2 cut(s)	669, 987
SacI	GAGCTC	2 cut(s)	425, 1089
SapI	GCTCTTC	1 cut(s)	271
SaqAI	TTAA	4 cut(s)	246, 618, 707, 1104
SatI	GCNGC	3 cut(s)	285, 1095, 1234
Sau3AI	GATC	6 cut(s)	76, 728, 755, 826, 919, 1334
Sau96I	GGNCC	4 cut(s)	216, 254, 299, 1047
ScaI	AGTACT	1 cut(s)	137
SchI	GAGTC	2 cut(s)	230, 743
ScrFI	CCNGG	2 cut(s)	108, 258
SduI	GDGCHC	3 cut(s)	425, 1089, 1290
SfaNI	GCATC	4 cut(s)	454, 775, 875, 1162
SfuI	TTCGAA	1 cut(s)	492
SinI	GGWCC	1 cut(s)	299
SmiMI	CAYNNNNRTG	2 cut(s)	669, 987
SmlI	CTYRAG	1 cut(s)	1103
SmoI	CTYRAG	1 cut(s)	1103
Sse9I	AATT	7 cut(s)	101, 367, 484, 612, 619, 708, 880
SsiI	CCGC	1 cut(s)	1233
SspMI	CTAG	4 cut(s)	225, 479, 800, 1316
SstI	GAGCTC	2 cut(s)	425, 1089
StyD4I	CCNGG	2 cut(s)	106, 256
StyI	CCWWGG	4 cut(s)	700, 899, 1010, 1208
TaaI	ACNGT	5 cut(s)	121, 154, 507, 959, 1273
TaiI	ACGT	2 cut(s)	739, 969
TaqI	TCGA	4 cut(s)	492, 727, 825, 1306
TasI	AATT	7 cut(s)	101, 367, 484, 612, 619, 708, 880
TatI	WGTACW	2 cut(s)	135, 507
TauI	GCSGC	1 cut(s)	1236
TfiI	GAWTC	7 cut(s)	93, 197, 340, 383, 716, 1052, 1133
Tru1I	TTAA	4 cut(s)	246, 618, 707, 1104
Tru9I	TTAA	4 cut(s)	246, 618, 707, 1104
TscAI	CASTG	8 cut(s)	780, 868, 934, 1030, 1126, 1135, 1143, 1276
TseFI	GTSAC	5 cut(s)	649, 746, 1147, 1267, 1273
TseI	GCWGC	2 cut(s)	284, 1094
Tsp45I	GTSAC	5 cut(s)	649, 746, 1147, 1267, 1273
TspDTI	ATGAA	3 cut(s)	296, 396, 1312
TspGWI	ACGGA	1 cut(s)	759
TspRI	CASTG	8 cut(s)	780, 868, 934, 1030, 1126, 1135, 1143, 1276
Vha464I	CTTAAG	1 cut(s)	1103
VpaK11BI	GGWCC	1 cut(s)	299
VspI	ATTAAT	1 cut(s)	618
XapI	RAATTY	1 cut(s)	367
XbaI	TCTAGA	1 cut(s)	224
XceI	RCATGY	1 cut(s)	1023
XcmI	CCANNNNNNNNNTGG	1 cut(s)	237
XmiI	GTMKAC	1 cut(s)	434
XspI	CTAG	4 cut(s)	225, 479, 800, 1316
ZrmI	AGTACT	1 cut(s)	137
Zsp2I	ATGCAT	1 cut(s)	475

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.