FvH4_7g19810

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004331
Physical Location & Seq
Reverse (-)
16264556 .. 16270991
6436 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g19810.t3

Sequence Viewer

Length: 1377 bp
ATGGGTTACCAAAACTCTCTATTTGCATCTGTGTGCATTTTGCTACTTTCATTTGGAGCTTGTTTTGCTTCGTCTATTCAAGATCAAGTGAGGGATAGAATAACACAGTTACCAGGGCAGCCAGCCAATGTGGGGTTTCGTCAGTATTCTGGTTATGTCACTGCCAATAAGCAAGCTGGGAGAGCATTGTTTTACTGGTTGGTTGAGTCCCCAGCGCAGCGAGGACCGGAGTCCAGACCGCTAGTGTTGTGGCTCACTGGTGGTCCAGGTTGCTCTTCTGTTGCTTATGGAGCAGCTGAAGAGATTGGACCTTTTCATATTAGACCTGATGGGAAGACCCTTTACTTGAATCCATATGCTTGGAACAATTTGGCAAATTTGCTTTTCCTTGAATCGCCAGCTGGTGTTGGATTTTCATATACCAATACATCTTCAGATTTGTATACAGCTGGGGACCAGAGAACAGCTGAAGATGCATATACATTTCTGGTCAATTGGTTCGAAAGGTTTCCTCAGTACAAGCACAGAGATTTCTACATTGCTGGAGATAGTTATGCAGGTCATTATGTTCCTCAGTTGTCTCAGTTGGTTTATGAGAGAAATAAGGGAATTAAGAATCCAGATATCAATTTTAAGGGGTTTTTGGTAGGAAATGCTGTGACCGATGATTACTATGATTTTATTGGCACATTCGAATACTGGTGGACACATGGTTTAATTTCTGATTCCACCTATCGGGCACTGCGAGTTAAGTGTCACTTGGAATTCGCTCAGCATCTGTCACTGGCTTGCACGGAGGCCCTCGAACTAGCAGATTCAGAGTTCGGAAACATTGACCCTACCAGCATTTTCACCCGTCCTTGTGGTAGTGCTGCAGCACTGAAGGGCAACTTGAGTGGTCATTATCCATGGAAGTCCAGAGCATATGATCCATGCGCTGAGAGGTATTCTGAAAAGTATTTCAACCATCCAGAAGTTCAAAAGGCACTTCATGCAAATGTAACTGGACTTTCATACCCATGGAAAACATGCAGTGATATTATTGGAAACAACTGGTCGGATTCTCCACGGTCTATGCTTCCAATTTATCAAGAGCTTATTGCTGCTGGTCTCAAGATATGGGTCTACAGCGGAGATACTGATGCAGTGGTTCCTGTTACTGCCACGCGTTACTCCATTGACGCCCTTAAGTTACCGACCATCGCCAACTGGTCCCCATGGTACGATTCCGGCAAGGTTGGTGGCCGGAGCCAAATATATAAGGGGCTGACATTAGTTACCATCACCGGAGCTGGACATCTGGTCCCACTCCTTCGACCTCGGGAAGCTTTCATTCTTTTCAAATCATTTTTGGAGAACAAGCCCATGCCTAGTTAA

Protein Analysis

459

Amino Acids

51.15

Weight (kDa)

6.56

Isoelectric Point (pI)

39.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 38 - 450 4.6e-143 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 548
AccI GTMKAC 2 cut(s) 443, 1125
AccII CGCG 1 cut(s) 1168
AciI CCGC 2 cut(s) 239, 1131
AclWI GGATC 1 cut(s) 923
AcoI YGGCCR 1 cut(s) 1243
AcsI RAATTY 2 cut(s) 376, 764
AcuI CTGAAG 4 cut(s) 318, 417, 489, 902
AcyI GRCGYC 1 cut(s) 1182
AfaI GTAC 2 cut(s) 518, 1223
AfiI CCNNNNNNNGG 2 cut(s) 132, 735
AflII CTTAAG 1 cut(s) 1187
AflIII ACRYGT 1 cut(s) 1166
AgsI TTSAA 6 cut(s) 80, 349, 392, 964, 980, 1342
AhdI GACNNNNNGTC 1 cut(s) 1301
AjnI CCWGG 2 cut(s) 112, 265
AluBI AGCT 9 cut(s) 59, 176, 296, 401, 449, 467, 1096, 1292, 1328
AluI AGCT 9 cut(s) 59, 176, 296, 401, 449, 467, 1096, 1292, 1328
Alw26I GTCTC 2 cut(s) 585, 1115
AlwI GGATC 1 cut(s) 923
AlwNI CAGNNNCTG 1 cut(s) 778
Ama87I CYCGRG 1 cut(s) 1320
AoxI GGCC 2 cut(s) 798, 1243
ApeKI GCWGC 6 cut(s) 118, 217, 293, 872, 875, 1103
ApoI RAATTY 2 cut(s) 376, 764
Asp700I GAANNNNTTC 1 cut(s) 507
AspLEI GCGC 2 cut(s) 217, 938
AspS9I GGNCC 7 cut(s) 224, 263, 308, 454, 799, 1212, 1303
AsuHPI GGTGA 2 cut(s) 844, 1276
AsuII TTCGAA 2 cut(s) 501, 693
AvaI CYCGRG 1 cut(s) 1320
AvaII GGWCC 6 cut(s) 224, 263, 308, 454, 1212, 1303
BaeGI GKGCMC 1 cut(s) 742
BarI GAAGNNNNNNTAC 2 cut(s) 326, 358
BbsI GAAGAC 1 cut(s) 341
BbvI GCAGC 6 cut(s) 130, 229, 305, 859, 887, 1090
BccI CCATC 4 cut(s) 323, 975, 1208, 1289
BciT130I CCWGG 2 cut(s) 114, 267
BcoDI GTCTC 2 cut(s) 585, 1115
BfaI CTAG 3 cut(s) 242, 809, 1371
BfmI CTRYAG 2 cut(s) 873, 1126
BfrI CTTAAG 1 cut(s) 1187
BfuAI ACCTGC 1 cut(s) 548
BisI GCNGC 6 cut(s) 119, 218, 294, 873, 876, 1104
BlpI GCTNAGC 1 cut(s) 771
BlsI GCNGC 6 cut(s) 120, 219, 295, 874, 877, 1105
Bme1390I CCNGG 2 cut(s) 114, 267
Bme18I GGWCC 6 cut(s) 224, 263, 308, 454, 1212, 1303
BmeRI GACNNNNNGTC 1 cut(s) 1301
BmeT110I CYCGRG 1 cut(s) 1320
BmgT120I GGNCC 7 cut(s) 224, 263, 308, 454, 799, 1212, 1303
BmiI GGNNCC 5 cut(s) 455, 1152, 1214, 1250, 1305
BmrFI CCNGG 2 cut(s) 114, 267
BmsI GCATC 4 cut(s) 35, 463, 784, 1132
BoxI GACNNNNGTC 1 cut(s) 229
BpiI GAAGAC 1 cut(s) 341
BpmI CTGGAG 1 cut(s) 564
Bpu1102I GCTNAGC 1 cut(s) 771
Bpu14I TTCGAA 2 cut(s) 501, 693
BpuEI CTTGAG 2 cut(s) 913, 1097
BsaHI GRCGYC 1 cut(s) 1182
BsaI GGTCTC 1 cut(s) 1115
BsaJI CCNNGG 6 cut(s) 113, 908, 1019, 1067, 1217, 1319
BsaWI WCCGGW 2 cut(s) 226, 1286
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 735
Bse1I ACTGG 7 cut(s) 200, 262, 704, 789, 1009, 1058, 1214
Bse3DI GCAATG 1 cut(s) 537
BseBI CCWGG 2 cut(s) 114, 267
BseDI CCNNGG 6 cut(s) 113, 908, 1019, 1067, 1217, 1319
BseGI GGATG 1 cut(s) 967
BseLI CCNNNNNNNGG 2 cut(s) 132, 735
BseMI GCAATG 1 cut(s) 537
BseMII CTCAG 5 cut(s) 527, 587, 596, 785, 930
BseNI ACTGG 7 cut(s) 200, 262, 704, 789, 1009, 1058, 1214
BseSI GKGCMC 1 cut(s) 742
BseXI GCAGC 6 cut(s) 130, 229, 305, 859, 887, 1090
BseYI CCCAGC 3 cut(s) 176, 211, 449
Bsh1236I CGCG 1 cut(s) 1168
BshFI GGCC 2 cut(s) 800, 1245
BsiHKCI CYCGRG 1 cut(s) 1320
BsiSI CCGG 4 cut(s) 227, 1230, 1246, 1287
BslFI GGGAC 4 cut(s) 193, 467, 1198, 1289
BslI CCNNNNNNNGG 2 cut(s) 132, 735
BsmAI GTCTC 2 cut(s) 585, 1115
BsmFI GGGAC 4 cut(s) 193, 467, 1198, 1289
BsnI GGCC 2 cut(s) 800, 1245
Bso31I GGTCTC 1 cut(s) 1115
BsoBI CYCGRG 1 cut(s) 1320
Bsp119I TTCGAA 2 cut(s) 501, 693
Bsp1286I GDGCHC 1 cut(s) 742
Bsp143I GATC 2 cut(s) 82, 928
Bsp1720I GCTNAGC 1 cut(s) 771
Bsp19I CCATGG 3 cut(s) 908, 1019, 1217
BspACI CCGC 2 cut(s) 239, 1131
BspANI GGCC 2 cut(s) 800, 1245
BspCNI CTCAG 5 cut(s) 526, 586, 595, 784, 931
BspFNI CGCG 1 cut(s) 1168
BspLI GGNNCC 5 cut(s) 455, 1152, 1214, 1250, 1305
BspMAI CTGCAG 1 cut(s) 877
BspMI ACCTGC 1 cut(s) 548
BspPI GGATC 1 cut(s) 923
BspQI GCTCTTC 1 cut(s) 280
BspT104I TTCGAA 2 cut(s) 501, 693
BspTI CTTAAG 1 cut(s) 1187
BspTNI GGTCTC 1 cut(s) 1115
BsrDI GCAATG 1 cut(s) 537
BsrI ACTGG 7 cut(s) 200, 262, 704, 789, 1009, 1058, 1214
BssECI CCNNGG 6 cut(s) 113, 908, 1019, 1067, 1217, 1319
BssMI GATC 2 cut(s) 82, 928
BssNAI GTATAC 1 cut(s) 444
BssNI GRCGYC 1 cut(s) 1182
BssT1I CCWWGG 3 cut(s) 908, 1019, 1217
Bst1107I GTATAC 1 cut(s) 444
Bst2UI CCWGG 2 cut(s) 114, 267
Bst4CI ACNGT 2 cut(s) 108, 1071
Bst6I CTCTTC 2 cut(s) 280, 294
BstACI GRCGYC 1 cut(s) 1182
BstAFI CTTAAG 1 cut(s) 1187
BstAPI GCANNNNNTGC 1 cut(s) 992
BstBI TTCGAA 2 cut(s) 501, 693
BstC8I GCNNGC 4 cut(s) 123, 174, 399, 790
BstDEI CTNAG 5 cut(s) 513, 573, 582, 771, 939
BstDSI CCRYGG 4 cut(s) 908, 1019, 1067, 1217
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 1 cut(s) 967
BstFNI CGCG 1 cut(s) 1168
BstHHI GCGC 2 cut(s) 217, 938
BstKTI GATC 2 cut(s) 85, 931
BstMAI GTCTC 2 cut(s) 585, 1115
BstMBI GATC 2 cut(s) 82, 928
BstMWI GCNNNNNNNGC 5 cut(s) 65, 182, 290, 473, 992
BstNI CCWGG 2 cut(s) 114, 267
BstNSI RCATGY 1 cut(s) 1032
BstPAI GACNNNNGTC 1 cut(s) 229
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 2 cut(s) 112, 265
BstSFI CTRYAG 2 cut(s) 873, 1126
BstSLI GKGCMC 1 cut(s) 742
BstUI CGCG 1 cut(s) 1168
BstV1I GCAGC 6 cut(s) 130, 229, 305, 859, 887, 1090
BstV2I GAAGAC 1 cut(s) 341
BstXI CCANNNNNNTGG 1 cut(s) 360
BstZ17I GTATAC 1 cut(s) 444
BsuRI GGCC 2 cut(s) 800, 1245
BtgI CCRYGG 4 cut(s) 908, 1019, 1067, 1217
BtgZI GCGATG 1 cut(s) 1186
BtsCI GGATG 1 cut(s) 967
BtsI GCAGTG 4 cut(s) 159, 740, 1039, 1152
BtsIMutI CAGTG 7 cut(s) 159, 255, 740, 782, 878, 1039, 1152
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 4 cut(s) 123, 174, 399, 790
CaiI CAGNNNCTG 1 cut(s) 778
CfoI GCGC 2 cut(s) 217, 938
Cfr13I GGNCC 7 cut(s) 224, 263, 308, 454, 799, 1212, 1303
CseI GACGC 1 cut(s) 1190
Csp6I GTAC 2 cut(s) 517, 1222
CspCI CAANNNNNGTGG 4 cut(s) 877, 912, 1222, 1257
CviAII CATG 8 cut(s) 710, 909, 933, 992, 1020, 1029, 1218, 1366
CviQI GTAC 2 cut(s) 517, 1222
DdeI CTNAG 5 cut(s) 513, 573, 582, 771, 939
DpnI GATC 2 cut(s) 84, 930
DpnII GATC 2 cut(s) 82, 928
DriI GACNNNNNGTC 1 cut(s) 1301
EaeI YGGCCR 1 cut(s) 1243
Eam1104I CTCTTC 2 cut(s) 280, 294
Eam1105I GACNNNNNGTC 1 cut(s) 1301
EarI CTCTTC 2 cut(s) 280, 294
Eco130I CCWWGG 3 cut(s) 908, 1019, 1217
Eco31I GGTCTC 1 cut(s) 1115
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 6 cut(s) 224, 263, 308, 454, 1212, 1303
Eco57I CTGAAG 4 cut(s) 318, 417, 489, 902
Eco88I CYCGRG 1 cut(s) 1320
Eco91I GGTNACC 1 cut(s) 5
EcoO109I RGGNCCY 1 cut(s) 799
EcoO65I GGTNACC 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 112, 265
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 3 cut(s) 908, 1019, 1217
EcoT22I ATGCAT 1 cut(s) 478
ErhI CCWWGG 3 cut(s) 908, 1019, 1217
FaeI CATG 8 cut(s) 713, 912, 936, 995, 1023, 1032, 1221, 1369
FalI AAGNNNNNCTT 4 cut(s) 743, 775, 875, 907
FaqI GGGAC 4 cut(s) 193, 467, 1198, 1289
FatI CATG 8 cut(s) 709, 908, 932, 991, 1019, 1028, 1217, 1365
FauNDI CATATG 2 cut(s) 355, 925
FblI GTMKAC 2 cut(s) 443, 1125
Fnu4HI GCNGC 6 cut(s) 119, 218, 294, 873, 876, 1104
FokI GGATG 1 cut(s) 954
Fsp4HI GCNGC 6 cut(s) 119, 218, 294, 873, 876, 1104
FspBI CTAG 3 cut(s) 242, 809, 1371
GlaI GCGC 2 cut(s) 216, 937
GluI GCNGC 6 cut(s) 119, 218, 294, 873, 876, 1104
GsaI CCCAGC 3 cut(s) 180, 215, 453
GsuI CTGGAG 1 cut(s) 564
HaeIII GGCC 2 cut(s) 800, 1245
HapII CCGG 4 cut(s) 227, 1230, 1246, 1287
HgaI GACGC 1 cut(s) 1190
HhaI GCGC 2 cut(s) 217, 938
Hin1I GRCGYC 1 cut(s) 1182
Hin1II CATG 8 cut(s) 713, 912, 936, 995, 1023, 1032, 1221, 1369
Hin6I GCGC 2 cut(s) 215, 936
HinP1I GCGC 2 cut(s) 215, 936
HindIII AAGCTT 1 cut(s) 1326
HinfI GANTC 9 cut(s) 206, 230, 349, 392, 616, 725, 815, 1061, 1226
HpaII CCGG 4 cut(s) 227, 1230, 1246, 1287
HphI GGTGA 2 cut(s) 844, 1276
Hpy166II GTNNAC 3 cut(s) 444, 705, 1126
Hpy188I TCNGA 6 cut(s) 436, 724, 820, 827, 952, 1060
Hpy188III TCNNGA 8 cut(s) 80, 234, 620, 918, 971, 1091, 1114, 1322
Hpy8I GTNNAC 3 cut(s) 444, 705, 1126
HpyAV CCTTC 2 cut(s) 877, 1322
HpyCH4III ACNGT 2 cut(s) 108, 1071
HpyCH4V TGCA 9 cut(s) 26, 36, 476, 557, 792, 875, 995, 1032, 1145
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 182, 290, 473, 992
HpyF3I CTNAG 5 cut(s) 513, 573, 582, 771, 939
Hsp92I GRCGYC 1 cut(s) 1182
Hsp92II CATG 8 cut(s) 713, 912, 936, 995, 1023, 1032, 1221, 1369
HspAI GCGC 2 cut(s) 215, 936
Kzo9I GATC 2 cut(s) 82, 928
LguI GCTCTTC 1 cut(s) 280
LmnI GCTCC 4 cut(s) 56, 290, 1248, 1289
Lsp1109I GCAGC 6 cut(s) 130, 229, 305, 859, 887, 1090
LweI GCATC 4 cut(s) 35, 463, 784, 1132
MaeI CTAG 3 cut(s) 242, 809, 1371
MalI GATC 2 cut(s) 84, 930
MboI GATC 2 cut(s) 82, 928
MboII GAAGA 5 cut(s) 267, 311, 346, 423, 482
MfeI CAATTG 1 cut(s) 493
MhlI GDGCHC 1 cut(s) 742
MluCI AATT 8 cut(s) 367, 376, 493, 609, 628, 717, 764, 1083
MluI ACGCGT 1 cut(s) 1166
MlyI GAGTC 2 cut(s) 215, 239
MmeI TCCRAC 2 cut(s) 388, 1038
MnlI CCTC 8 cut(s) 84, 215, 522, 582, 790, 812, 936, 1329
Mph1103I ATGCAT 1 cut(s) 478
MroXI GAANNNNTTC 1 cut(s) 507
MseI TTAA 6 cut(s) 612, 633, 716, 750, 1188, 1375
MslI CAYNNNNRTG 3 cut(s) 31, 996, 1018
MspA1I CMGCKG 5 cut(s) 296, 401, 449, 467, 1131
MspCI CTTAAG 1 cut(s) 1187
MspI CCGG 4 cut(s) 227, 1230, 1246, 1287
MspR9I CCNGG 2 cut(s) 114, 267
MunI CAATTG 1 cut(s) 493
MvaI CCWGG 2 cut(s) 114, 267
MvnI CGCG 1 cut(s) 1168
MwoI GCNNNNNNNGC 5 cut(s) 65, 182, 290, 473, 992
NcoI CCATGG 3 cut(s) 908, 1019, 1217
NdeI CATATG 2 cut(s) 355, 925
NdeII GATC 2 cut(s) 82, 928
NlaIII CATG 8 cut(s) 713, 912, 936, 995, 1023, 1032, 1221, 1369
NlaIV GGNNCC 5 cut(s) 455, 1152, 1214, 1250, 1305
NmuCI GTSAC 4 cut(s) 157, 658, 755, 780
NsiI ATGCAT 1 cut(s) 478
NspI RCATGY 1 cut(s) 1032
NspV TTCGAA 2 cut(s) 501, 693
PciSI GCTCTTC 1 cut(s) 280
PcsI WCGNNNNNNNCGW 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 507
PfeI GAWTC 7 cut(s) 349, 392, 616, 725, 815, 1061, 1226
PkrI GCNGC 6 cut(s) 120, 219, 295, 874, 877, 1105
PleI GAGTC 2 cut(s) 214, 238
PpsI GAGTC 2 cut(s) 214, 238
PshAI GACNNNNGTC 1 cut(s) 229
Psp6I CCWGG 2 cut(s) 112, 265
PspEI GGTNACC 1 cut(s) 5
PspFI CCCAGC 3 cut(s) 176, 211, 449
PspGI CCWGG 2 cut(s) 112, 265
PspN4I GGNNCC 5 cut(s) 455, 1152, 1214, 1250, 1305
PspPI GGNCC 7 cut(s) 224, 263, 308, 454, 799, 1212, 1303
PstI CTGCAG 1 cut(s) 877
PstNI CAGNNNCTG 1 cut(s) 778
PvuII CAGCTG 4 cut(s) 296, 401, 449, 467
RsaI GTAC 2 cut(s) 518, 1223
RsaNI GTAC 2 cut(s) 517, 1222
RseI CAYNNNNRTG 3 cut(s) 31, 996, 1018
SapI GCTCTTC 1 cut(s) 280
SaqAI TTAA 6 cut(s) 612, 633, 716, 750, 1188, 1375
SatI GCNGC 6 cut(s) 119, 218, 294, 873, 876, 1104
Sau3AI GATC 2 cut(s) 82, 928
Sau96I GGNCC 7 cut(s) 224, 263, 308, 454, 799, 1212, 1303
SchI GAGTC 2 cut(s) 215, 239
ScrFI CCNGG 2 cut(s) 114, 267
SduI GDGCHC 1 cut(s) 742
SfaNI GCATC 4 cut(s) 35, 463, 784, 1132
SfcI CTRYAG 2 cut(s) 873, 1126
SfuI TTCGAA 2 cut(s) 501, 693
SinI GGWCC 6 cut(s) 224, 263, 308, 454, 1212, 1303
SmiMI CAYNNNNRTG 3 cut(s) 31, 996, 1018
SmlI CTYRAG 3 cut(s) 892, 1112, 1187
SmoI CTYRAG 3 cut(s) 892, 1112, 1187
Sse9I AATT 8 cut(s) 367, 376, 493, 609, 628, 717, 764, 1083
SsiI CCGC 2 cut(s) 239, 1131
SspMI CTAG 3 cut(s) 242, 809, 1371
StyD4I CCNGG 2 cut(s) 112, 265
StyI CCWWGG 3 cut(s) 908, 1019, 1217
TaaI ACNGT 2 cut(s) 108, 1071
TaqI TCGA 4 cut(s) 501, 693, 804, 1315
TaqII GACCGA 1 cut(s) 677
TasI AATT 8 cut(s) 367, 376, 493, 609, 628, 717, 764, 1083
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 7 cut(s) 349, 392, 616, 725, 815, 1061, 1226
Tru1I TTAA 6 cut(s) 612, 633, 716, 750, 1188, 1375
Tru9I TTAA 6 cut(s) 612, 633, 716, 750, 1188, 1375
TscAI CASTG 7 cut(s) 166, 262, 747, 789, 885, 1039, 1152
TseFI GTSAC 4 cut(s) 157, 658, 755, 780
TseI GCWGC 6 cut(s) 118, 217, 293, 872, 875, 1103
Tsp45I GTSAC 4 cut(s) 157, 658, 755, 780
TspDTI ATGAA 6 cut(s) 39, 305, 405, 980, 1002, 1321
TspGWI ACGGA 1 cut(s) 809
TspRI CASTG 7 cut(s) 166, 262, 747, 789, 885, 1039, 1152
Vha464I CTTAAG 1 cut(s) 1187
VpaK11BI GGWCC 6 cut(s) 224, 263, 308, 454, 1212, 1303
XapI RAATTY 2 cut(s) 376, 764
XceI RCATGY 1 cut(s) 1032
XmiI GTMKAC 2 cut(s) 443, 1125
XmnI GAANNNNTTC 1 cut(s) 507
XspI CTAG 3 cut(s) 242, 809, 1371
Zsp2I ATGCAT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.