Rorug01G0309200

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004331
Physical Location & Seq
Forward (+)
42559393 .. 42572099
12707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0309200.1

Sequence Viewer

Length: 5574 bp
ATGGCACCCAGAAACAGTCGTGGAAAGGGCAAAGGTGGAGAGAAGAAGAAGAAGGAAGAGAAGGTTCTTCCTGTTGTGATGGACATCACTGTGAACCTTCCAGATGAAAGCAGTGTTGTTTTGAAGCAGGGAATATCGACGGACAGGATCATCGACGTTCGCCAACTATTATCAGTCAACACAGAAACTTGTAACATCACCAATTTTTCACTATCCCATGAGGTAAGAGGGAAACGTTTAAAAGATACGGTGGACGTCTCCGCACTCAAGCCATGCGTCATCACCTTGGTCGAAGAGGACTATGATGAAGAGCGCGCCACCGCGCACGTGCGGCGCGTGCTGGACATCGTGGCCTGCACGACGAGCTTTGGCGCGTCACCGACGCCTCCGCCGACTCCAGCGAAGGACCAAAGCCTCAAGCCCGACGCCTCGTCCGCAGTCCTCTCCGGTAAAAATTCGCAGGATAATAAGACCGCCAAGAAATCCACCGTCGCCAAGCCTCAAGGCTCTGCCGGAGCCGATAAGCGTGACGTGGCGGTCGACTCGGACGCCGAGCTGAGCCACTCTTGCCTCAAGCTCGGTTCCTTCTACGACTTCTTCTCTCTCGCTCATCTCACTCCCCCTCTGCAATTTATCCGAAGGGTGACGAAACGACATGTCGATGAGATTTCCGGCGATGATCACCTGTTTTCTCTTGAAGTGAAGCTTTGCAATGGGAAGGTGGTTCTGGTTGAAGCTTGCAGAAAGGGATTCTATAGTGTAGGGAAGCAGCGCATTCTGTGTCACAACCTCGTCGATTTGCTACGCCAGCTTAGTAGAGCTTTTGACAATGCTTACGATGATCTGCTGAAAGCATTTTTGGAACGTAATAAGTTTGGGAATCTTCCCTATGGCTTTCGAGCAAACACATGGCTTGTACCGCCTATTGCAGGGCAGTCGCCTTCAGGTTTCCCTGCTCTTCCTGTGGAGGATGAAGCTTGGGGAGGAAATGGAGGGGGTCTAGGAAGGGATGGGCAATTTGATCTGATTCCTTGGGCTAATGAGTTTTGGTATATTGCATCTATGCCTTGCAAGACGGCGGAGGAGAGGCAGGTTCGAGATAGGAAGGCATTCCTTCTTCACAGCCTATTTGTTGACGTTTCCATTTTTAGAGCCATTAAGGCTGTGCAGCATGTGATGGCAAAGCCGGAATTAACATGTTCAGCTGCAAACAGTGAGGTTCTTTATACTGAGAGAGTAGGGGACCTGAATGTGATAGTAACAAAGGATGTTAACAATGCGAGCTGTAAGGTAGATACTAAAATTGATGGAATTCAGGCAACTGGAGTGGATAAAAAGAATCTAGCACAACGAAACCTTCTTAAAGGGATCACTGCTGACGAGAACACCGCTGCACATGATGTCACCACTCTAGGTGTTGTCAATGTCCGATATTGTGGTCACATTGCAGTTGTGAAAGTTGAGGGCAAAGAAACTAAGAAAGTCGGTTCTCCATCCCAAAGCATTGACCTCCTTGATCAACCTGAAGGTGGTGCCAATGCCCTTAATATCAACAGTTTGAGATTACTACTTCACACTACTACACCTTCAGAGCAGAATAAACTGGCATCACATGGGCAAGGTCTGGAACATGAAGAGCTTTCTGCTTCCTGCGCTTTTGTTGAGAAGCTATTAGAAGAAAGTCTTGCTAAGCTTGACAAAGAGGAGCTAGATTCTGATAGTTTTGTGAGATGGGAACTAGGAGCTTGCTGGATACAACACTTGCAAGACCAAAAAAATACAGACAAGGATAAGAAACCATTGGGTGGGAAGGCTAAGAATGAGATGAAGGTCGAGGGACTTGGGACACCTCTTAAATCCCTTAAGAACACGAAGAAGAAATCAGATGGAGCCAATATTAAAGTGCAGTCTGATAGCTCAAAGTCGCATGCAGATGGTGTTGTGGGAGAAGTAGAAAATGCTATAGGTTCTGTGGAATCAAAGTTTGAAACCAGTGCTAAAGAAAATGAGCTTGTGCTGACAGAGATGCTCTCTGACGCAGCCTTTGCTCGACTAAAAGAATCAGAAACTGGACTTCATTGCAAGTCTCTGCAAGAACTGATCGACTTATCTCAGAAGTATTACTCTGAAGTTGCTCTTCCAAAACTGGTAGCCGATTTTGGTTCATTGGAACTTTCACCAGTTGATGGTCGAACTTTAACTGATTTCATGCATACTAGAGGTCTTCGGATGCGTTCTCTGGGGCAAGTTGTCAAGCTATCGGAGAAGCTATCACATGTGCAGTCCCTTTGCATTCATGAGATGATAGTGCGAGCTTTTAAGCACATACTTCAGGCAGTGATTGCTGCTGTTAATAACACTGAGAAAATGGCTGCCTCGATTGCTGCTGCATTGAATCTTATGTTGGGGGCTCCAGAAAATGAAGAATTCAATAAGTCTTTCAATGTGCATTCCCTTGTGTGGAGATGGCTGGAAGTATTCTTGCGAAAGAGATATGGATGGGATGTTAGCAGCTTCAACTACAATGATGTGAGGAGATTTGCGATTCTACGTGGCCTGTGCCATAAGGTGGGCATTGAGATGGTTCCACGAGATTTTGATATGGAGTCTCCAAATCCATTTCAGAGTTCTGATATTGTCAGCTTAGTTCCAGTACATAAGCAAGCTGCATGCTCTTCGGCAGATGGTAGGCAACTATTGGAGTCATCAAAAACAGCCTTAGATAAGGGAAAACTTGAAGATGCAGTTGCATATGGCACTAAGGCTCTTGCAAAGCTGGTAGCAGTTTGTGGTCCATACCATCGAATGACAGCAGGAGCTTACAGCCTCCTTGCTGTAGTTTTATATCACACTGGAGATTTTAATCAGGCTACAATTTATCAGCAAAAGGCTCTGGATATCAATGAGAGAGAATTAGGGCTTGACCATCCAGACACCATGAAGAGTTATGGGGATCTTGCTGTGTTCTATTACAGACTTCAACATACAGAGCTGGCTCTCAAATATGTGAAACGTGCTCTGTATCTCTTGCATCTCACATGTGGACCATCTCATCCAAACACTGCTGCAACATACATCAACGTGGCTATGATGGAAGAAGGCCTGGGAAATGTACATGTTGCCCTCAGATATCTACACAAGGCTTTGAAGTGTAATCAGAAGTTACTTGGCCCAGATCATATTCAGACAGCGGCTAGTTACCATGCTATTGCAATTGCACTCTCATTGATGGAAGCGTACCCTTTAAGTGTTCAGCATGAGCAGACAACATTGCAAATTCTGCGTGCAAAGCTTGGACCAGATGATCTACGTACACAAGATGCTGCTGCCTGGCTTGAGTACTTTGAGTCCAAGGCTTTTGAACAGCAAGAAGCTGCAAGAAATGGGACTCGAAAACCAGATGCATCTATAGCCAGCAAAGGCCACTTAAGCGTATCAGATTTGCTTGACTACATCAATCCCACTCATGATGCTAAAGGGAGAGATGCGGCAATGAAGAGGAAAACCTACATAACAAAGTTGAAGGAAAAATCCTATCAAACTATCAGCTCAGCAAGTTCTGACGAATCTTCAAAAGAAACCACAAAAGAGGTTTCAGACGAAGAAACACATACACTTGAACCAAGAGAAAGAACAGATCCCATCCAAGAAAACATCCCGGCTTCAGTTGAACCCCAGCATGTTGTAGAAGAGATTCCTGAGGAAAATTCAAACATGAGCTCTTCTGAAACACATGTTGAAGGGGAAGATGACTGGCATCCAGTTCAAAGGCCTAGATCAGCGGGTTCATATGGGCGGCGTGTAAAGCAGCGGCGGGCAGCTATTGGGAAGGTTTATAGTTACCAGAAGAAATATGTAGACGCTGATATGGATTACCCTTCAGTGAAGAATACTAACCAAAACAGTAGGTATTACCTTCTAAAGAAAAGGCCAACATCTCATGGAAGTTATACAGAAAATCAGATTGCGAATCCCTCCCAAGGTACTAAATTTGGACGGAGAATGGTAAAAGCTGTAGCATACAGAGTCAAGTCTATGCCTTTGTCTACCAAAGTTGTTGCAGCAGAAAGTTTGAGTTCCTCATCAGAAGTCAGTCCAAATGTTTCACCACATGGGATTGGTCCAGTTAAAGGTTCTATAGTCAGTCTTGGAAAATCTCCTTCATACAAGGAAGTAGCATTGGCTCCACCAGGTAGTATTGCTAAAATGCAAACTGTTTTCCCTCAGAGTGGCATTCCAGATAACCATGAGCATGGTATCCAAAGACAAGAAGAGAAAATAATTGAAATCAAAGGAGATTCCAAACCAAAGGCTGTAGTTGAAACTATACTTGAAGAAAAGAAGGATCCTGTATTGGTTTCCACAGATGTGTTACGAGAGAAAACTGGACCTGTTGAGAAGAAAGAAGATACTAATTCACCTGAAGTGAAGGAAGATAAAAATTCACTGATATTCGGGACCGTGGATGAACTTGGATCCAGTGCTGTTAAGTTTCAAGAGGCAGCAGTTGATAACGTATTAGTAGATGGACTGCCAAAATCCATTGATTCTGCCAAGGAGGGACTCTGTGAGAACAATCCATCTGGCACTTCTGAATTGCATGATTCCAACTCCACTTTGCAAGGAGTAGATGATTTGGAGGCATCCTCGCTTGTTACTTCAGCTGATACTCGGGGATTGCCAAATAAGAAGTTGTCTGCATCGGCAGCTCCATTCAATCCCTCACCATCCACTCGTGCTGCTCCGGTTCCCATGAACATTGCTATACCTTCTGGTGCGGGTTCTGTCCCAACCATTGCACCTTGGCCAGTAAACCTGAATATTCACCCAGGGGCAGCTACTGTCTTACCCACAGTTAATCCCATGTGCTCCTCTCCTCATCATCCATACCCTTCTCCCCCTGCTACCCCGAATATCATACAGCCATTGCCATTTATGTTCCCTCCTCCTTACAGTCAACCCCAAGTTGTACGAACCAGTACCTTTCCTGTGACTAGTGCATTTCATCCAAATCATTACACATGGCAGTGCAATGTGAATCCTAATGTATCCGAGTTTGTACCTACAACAGTTTGGCATGGTTGCCATCCAATGGAATTCTCTGCCCCAGCACCTGTTGTTGAGCCAATTTCTGATCCCTCTCCCTTGGAGTCAAAAATTCAGAACGATGATTCTGCTCCAGTTCTGCCAGTGGACATTGATAATGTGGGGGAAACTAAGAAAGAAGCGGACATTCTAACATCAGAGGCAACTTGTAATCCAATTGAAAGTGTCAAGGAAGTTGGTCCAGATCTTTGTGGGGTTGAGAATGCTCAGAACGAACCATGTGACAGCCCAAATGGAAAAGTTGGAAGTAGCGCTGAAAGGACGACTGATGGAGAGAAAACCTTCAGCATTTTGATAAGGGGCAGAAGAAACCGAAAGCAGACTCTGAGAATGCCAGTAAGTTTGCTCAGTCGACCATATGGCTCACAGTCATTCAAAGTCATATATAACAGGGTGGTCAGGGGGAATGATGCGTCCAAACCCATCAGCTTTTCATCTAGTGAAAACTGCACAGCTACTGCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

1857

Amino Acids

203.4

Weight (kDa)

6.48

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 47 - 117 9.2e-11 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 725 - 867 2.5e-24 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 907 - 969 2.3e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 909 - 1007 1.8e-07 Nephrocystin-3 TPR domain
TPR_10 PF13374 937 - 978 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 937 - 1007 1.5e-12 Tetratricopeptide repeat
TPR_10 PF13374 1021 - 1061 3.4e-07 Tetratricopeptide repeat
TPR_12 PF13424 1021 - 1095 5.3e-12 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 536
AatII GACGTC 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 1081
AccB1I GGYRCC 2 cut(s) 4, 1532
AccB7I CCANNNNNTGG 4 cut(s) 1805, 2186, 2569, 5092
AccI GTMKAC 4 cut(s) 540, 3858, 4046, 5458
AccII CGCG 4 cut(s) 315, 323, 336, 374
AclI AACGTT 1 cut(s) 235
AclWI GGATC 9 cut(s) 155, 1376, 2961, 3632, 4340, 4353, 4470, 4483, 5129
AcoI YGGCCR 1 cut(s) 4805
AcsI RAATTY 9 cut(s) 454, 1311, 2426, 3276, 3706, 3989, 4441, 5096, 5157
AcvI CACGTG 1 cut(s) 328
AcyI GRCGYC 4 cut(s) 255, 383, 426, 549
AfeI AGCGCT 1 cut(s) 5359
AflII CTTAAG 2 cut(s) 1862, 3427
AflIII ACRYGT 6 cut(s) 655, 1196, 2275, 3038, 3115, 3733
AhdI GACNNNNNGTC 1 cut(s) 430
AhlI ACTAGT 1 cut(s) 4994
AjiI CACGTC 1 cut(s) 532
AjnI CCWGG 4 cut(s) 3102, 3329, 4189, 4828
AjuI GAANNNNNNNTTGG 4 cut(s) 842, 874, 2387, 2419
AleI CACNNNNGTG 1 cut(s) 4367
Alw21I GWGCWC 3 cut(s) 3019, 3722, 4871
Alw26I GTCTC 3 cut(s) 262, 2091, 2613
AlwI GGATC 9 cut(s) 155, 1376, 2961, 3632, 4340, 4353, 4470, 4483, 5129
AlwNI CAGNNNCTG 5 cut(s) 2069, 4841, 5114, 5431, 5564
Ama87I CYCGRG 1 cut(s) 4671
Aor51HI AGCGCT 1 cut(s) 5359
AoxI GGCC 8 cut(s) 351, 2554, 3100, 3169, 3421, 3770, 3929, 4805
ApoI RAATTY 9 cut(s) 454, 1311, 2426, 3276, 3706, 3989, 4441, 5096, 5157
ArsI GACNNNNNNTTYG 2 cut(s) 2027, 2059
Asp700I GAANNNNTTC 3 cut(s) 1109, 3693, 5387
AspLEI GCGC 8 cut(s) 315, 317, 325, 336, 374, 774, 1655, 5360
AsuC2I CCSGG 1 cut(s) 3659
AvaI CYCGRG 1 cut(s) 4671
AvaII GGWCC 9 cut(s) 406, 1243, 2792, 3044, 3296, 4121, 4388, 4458, 5285
AxyI CCTNAGG 1 cut(s) 3699
BalI TGGCCA 1 cut(s) 4807
BamHI GGATCC 2 cut(s) 4345, 4475
BanI GGYRCC 2 cut(s) 4, 1532
BanII GRGCYC 2 cut(s) 2413, 3722
BauI CACGAG 2 cut(s) 2589, 4734
BbrPI CACGTG 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 2216
Bbv12I GWGCWC 3 cut(s) 3019, 3722, 4871
BceAI ACGGC 1 cut(s) 1092
BcgI CGANNNNNNTGC 6 cut(s) 918, 952, 2658, 2692, 5156, 5190
BciT130I CCWGG 4 cut(s) 3104, 3331, 4191, 4830
BciVI GTATCC 3 cut(s) 1746, 4268, 5059
BclI TGATCA 2 cut(s) 679, 1516
BcnI CCSGG 1 cut(s) 3659
BcoDI GTCTC 3 cut(s) 262, 2091, 2613
BcuI ACTAGT 1 cut(s) 4994
BfmI CTRYAG 7 cut(s) 754, 1962, 2835, 3408, 4014, 4137, 4314
BfoI RGCGCY 1 cut(s) 5361
BfrI CTTAAG 2 cut(s) 1862, 3427
BfuAI ACCTGC 1 cut(s) 1081
BfuI GTATCC 3 cut(s) 1746, 4268, 5059
BglI GCCNNNNNGGC 1 cut(s) 1160
BglII AGATCT 1 cut(s) 5290
BlpI GCTNAGC 3 cut(s) 557, 1689, 3550
BmcAI AGTACT 1 cut(s) 3341
Bme1390I CCNGG 5 cut(s) 3104, 3331, 3659, 4191, 4830
Bme18I GGWCC 9 cut(s) 406, 1243, 2792, 3044, 3296, 4121, 4388, 4458, 5285
BmeRI GACNNNNNGTC 1 cut(s) 430
BmeT110I CYCGRG 1 cut(s) 4671
BmgBI CACGTC 1 cut(s) 532
BmrFI CCNGG 5 cut(s) 3104, 3331, 3659, 4191, 4830
BpiI GAAGAC 1 cut(s) 2216
BplI GAGNNNNNCTC 4 cut(s) 2015, 2047, 4631, 4663
BpmI CTGGAG 5 cut(s) 381, 1344, 2397, 2874, 5163
Bpu1102I GCTNAGC 3 cut(s) 557, 1689, 3550
BpuEI CTTGAG 5 cut(s) 251, 401, 486, 557, 3356
BpuMI CCSGG 1 cut(s) 3659
BsaAI YACGTR 3 cut(s) 328, 2552, 3311
BsaBI GATNNNNATC 2 cut(s) 83, 2862
BsaHI GRCGYC 4 cut(s) 255, 383, 426, 549
BsaWI WCCGGW 2 cut(s) 446, 4744
Bse21I CCTNAGG 1 cut(s) 3699
Bse3DI GCAATG 9 cut(s) 718, 1443, 2077, 3269, 3498, 4758, 4794, 4925, 5038
Bse8I GATNNNNATC 2 cut(s) 83, 2862
BseBI CCWGG 4 cut(s) 3104, 3331, 4191, 4830
BseJI GATNNNNATC 2 cut(s) 83, 2862
BseMI GCAATG 9 cut(s) 718, 1443, 2077, 3269, 3498, 4758, 4794, 4925, 5038
BsePI GCGCGC 1 cut(s) 313
BseRI GAGGAG 6 cut(s) 1097, 1718, 2548, 4861, 4866, 4935
BseYI CCCAGC 2 cut(s) 3675, 5107
BsgI GTGCAG 6 cut(s) 340, 1187, 1377, 1925, 2300, 5539
Bsh1236I CGCG 4 cut(s) 315, 323, 336, 374
Bsh1285I CGRYCG 1 cut(s) 540
BshFI GGCC 8 cut(s) 353, 2556, 3102, 3171, 3423, 3772, 3931, 4807
BshNI GGYRCC 2 cut(s) 4, 1532
BsiEI CGRYCG 1 cut(s) 540
BsiHKAI GWGCWC 3 cut(s) 3019, 3722, 4871
BsiHKCI CYCGRG 1 cut(s) 4671
BsiSI CCGG 6 cut(s) 447, 513, 672, 1187, 3659, 4745
BslFI GGGAC 8 cut(s) 1256, 1851, 1858, 2269, 3400, 4471, 4575, 4772
BsmAI GTCTC 3 cut(s) 262, 2091, 2613
BsmBI CGTCTC 1 cut(s) 262
BsmFI GGGAC 8 cut(s) 1256, 1851, 1858, 2269, 3400, 4471, 4575, 4772
BsmI GAATGC 7 cut(s) 774, 1109, 2292, 2449, 4233, 5314, 5442
BsnI GGCC 8 cut(s) 353, 2556, 3102, 3171, 3423, 3772, 3931, 4807
BsoBI CYCGRG 1 cut(s) 4671
Bsp1286I GDGCHC 4 cut(s) 2413, 3019, 3722, 4871
Bsp1407I TGTACA 1 cut(s) 3112
Bsp1720I GCTNAGC 3 cut(s) 557, 1689, 3550
BspANI GGCC 8 cut(s) 353, 2556, 3102, 3171, 3423, 3772, 3931, 4807
BspFNI CGCG 4 cut(s) 315, 323, 336, 374
BspHI TCATGA 2 cut(s) 2296, 3466
BspMI ACCTGC 1 cut(s) 1081
BspPI GGATC 9 cut(s) 155, 1376, 2961, 3632, 4340, 4353, 4470, 4483, 5129
BspQI GCTCTTC 6 cut(s) 303, 963, 1629, 2142, 2680, 3727
BspT107I GGYRCC 2 cut(s) 4, 1532
BspTI CTTAAG 2 cut(s) 1862, 3427
BsrDI GCAATG 9 cut(s) 718, 1443, 2077, 3269, 3498, 4758, 4794, 4925, 5038
BsrGI TGTACA 1 cut(s) 3112
BssHII GCGCGC 1 cut(s) 313
BssNI GRCGYC 4 cut(s) 255, 383, 426, 549
BssSI CACGAG 2 cut(s) 2589, 4734
BssT1I CCWWGG 7 cut(s) 285, 1031, 3351, 3979, 4554, 4802, 5145
Bst2BI CACGAG 2 cut(s) 2589, 4734
Bst2UI CCWGG 4 cut(s) 3104, 3331, 4191, 4830
BstACI GRCGYC 4 cut(s) 255, 383, 426, 549
BstAFI CTTAAG 2 cut(s) 1862, 3427
BstAPI GCANNNNNTGC 3 cut(s) 2045, 2342, 3280
BstAUI TGTACA 1 cut(s) 3112
BstBAI YACGTR 3 cut(s) 328, 2552, 3311
BstDSI CCRYGG 1 cut(s) 4461
BstENI CCTNNNNNAGG 1 cut(s) 927
BstFNI CGCG 4 cut(s) 315, 323, 336, 374
BstH2I RGCGCY 1 cut(s) 5361
BstHHI GCGC 8 cut(s) 315, 317, 325, 336, 374, 774, 1655, 5360
BstMAI GTCTC 3 cut(s) 262, 2091, 2613
BstMCI CGRYCG 1 cut(s) 540
BstNI CCWGG 4 cut(s) 3104, 3331, 4191, 4830
BstSCI CCNGG 5 cut(s) 3102, 3329, 3657, 4189, 4828
BstSFI CTRYAG 7 cut(s) 754, 1962, 2835, 3408, 4014, 4137, 4314
BstSNI TACGTA 1 cut(s) 3311
BstUI CGCG 4 cut(s) 315, 323, 336, 374
BstV2I GAAGAC 1 cut(s) 2216
BstX2I RGATCY 5 cut(s) 2953, 3637, 4345, 4475, 5290
BstXI CCANNNNNNTGG 1 cut(s) 4253
BstYI RGATCY 5 cut(s) 2953, 3637, 4345, 4475, 5290
Bsu36I CCTNAGG 1 cut(s) 3699
BsuI GTATCC 3 cut(s) 1746, 4268, 5059
BsuRI GGCC 8 cut(s) 353, 2556, 3102, 3171, 3423, 3772, 3931, 4807
BtgI CCRYGG 1 cut(s) 4461
BtgZI GCGATG 1 cut(s) 690
BtrI CACGTC 1 cut(s) 532
BtsI GCAGTG 5 cut(s) 118, 1371, 2343, 3060, 5033
BveI ACCTGC 1 cut(s) 1081
CaiI CAGNNNCTG 5 cut(s) 2069, 4841, 5114, 5431, 5564
CciI TCATGA 2 cut(s) 2296, 3466
CfoI GCGC 8 cut(s) 315, 317, 325, 336, 374, 774, 1655, 5360
CseI GACGC 8 cut(s) 265, 363, 391, 434, 557, 2045, 3869, 5508
CsiI ACCWGGT 1 cut(s) 4189
CspCI CAANNNNNGTGG 2 cut(s) 1308, 1343
DraI TTTAAA 1 cut(s) 240
DrdI GACNNNNNNGTC 1 cut(s) 536
DriI GACNNNNNGTC 1 cut(s) 430
DseDI GACNNNNNNGTC 1 cut(s) 536
EaeI YGGCCR 1 cut(s) 4805
Eam1105I GACNNNNNGTC 1 cut(s) 430
EciI GGCGGA 2 cut(s) 378, 1094
Ecl136II GAGCTC 1 cut(s) 3720
Eco105I TACGTA 1 cut(s) 3311
Eco130I CCWWGG 7 cut(s) 285, 1031, 3351, 3979, 4554, 4802, 5145
Eco147I AGGCCT 2 cut(s) 3102, 3772
Eco24I GRGCYC 2 cut(s) 2413, 3722
Eco32I GATATC 2 cut(s) 2899, 3131
Eco47I GGWCC 9 cut(s) 406, 1243, 2792, 3044, 3296, 4121, 4388, 4458, 5285
Eco47III AGCGCT 1 cut(s) 5359
Eco53kI GAGCTC 1 cut(s) 3720
Eco72I CACGTG 1 cut(s) 328
Eco81I CCTNAGG 1 cut(s) 3699
Eco88I CYCGRG 1 cut(s) 4671
EcoICRI GAGCTC 1 cut(s) 3720
EcoNI CCTNNNNNAGG 1 cut(s) 927
EcoO109I RGGNCCY 1 cut(s) 1243
EcoRI GAATTC 3 cut(s) 1311, 2426, 5096
EcoRII CCWGG 4 cut(s) 3102, 3329, 4189, 4828
EcoRV GATATC 2 cut(s) 2899, 3131
EcoT14I CCWWGG 7 cut(s) 285, 1031, 3351, 3979, 4554, 4802, 5145
EcoT22I ATGCAT 2 cut(s) 2214, 3406
EcoT38I GRGCYC 2 cut(s) 2413, 3722
ErhI CCWWGG 7 cut(s) 285, 1031, 3351, 3979, 4554, 4802, 5145
Esp3I CGTCTC 1 cut(s) 262
FalI AAGNNNNNCTT 6 cut(s) 690, 722, 1668, 1700, 2121, 2153
FaqI GGGAC 8 cut(s) 1256, 1851, 1858, 2269, 3400, 4471, 4575, 4772
FauI CCCGC 3 cut(s) 3775, 3807, 4771
FauNDI CATATG 3 cut(s) 2752, 3790, 5464
FbaI TGATCA 2 cut(s) 679, 1516
FblI GTMKAC 4 cut(s) 540, 3858, 4046, 5458
FriOI GRGCYC 2 cut(s) 2413, 3722
GlaI GCGC 8 cut(s) 314, 316, 324, 335, 373, 773, 1654, 5359
GsaI CCCAGC 2 cut(s) 3679, 5111
GsuI CTGGAG 5 cut(s) 381, 1344, 2397, 2874, 5163
HaeII RGCGCY 1 cut(s) 5361
HaeIII GGCC 8 cut(s) 353, 2556, 3102, 3171, 3423, 3772, 3931, 4807
HapII CCGG 6 cut(s) 447, 513, 672, 1187, 3659, 4745
HgaI GACGC 8 cut(s) 265, 363, 391, 434, 557, 2045, 3869, 5508
HhaI GCGC 8 cut(s) 315, 317, 325, 336, 374, 774, 1655, 5360
Hin1I GRCGYC 4 cut(s) 255, 383, 426, 549
Hin6I GCGC 8 cut(s) 313, 315, 323, 334, 372, 772, 1653, 5358
HinP1I GCGC 8 cut(s) 313, 315, 323, 334, 372, 772, 1653, 5358
HincII GTYRAC 6 cut(s) 178, 541, 1135, 1273, 4958, 5459
HindII GTYRAC 6 cut(s) 178, 541, 1135, 1273, 4958, 5459
HindIII AAGCTT 5 cut(s) 704, 735, 975, 1691, 3290
HpaI GTTAAC 1 cut(s) 1273
HpaII CCGG 6 cut(s) 447, 513, 672, 1187, 3659, 4745
Hpy99I CGWCG 7 cut(s) 142, 158, 364, 385, 428, 494, 797
Hsp92I GRCGYC 4 cut(s) 255, 383, 426, 549
HspAI GCGC 8 cut(s) 313, 315, 323, 334, 372, 772, 1653, 5358
Ksp22I TGATCA 2 cut(s) 679, 1516
KspAI GTTAAC 1 cut(s) 1273
LguI GCTCTTC 6 cut(s) 303, 963, 1629, 2142, 2680, 3727
MabI ACCWGGT 1 cut(s) 4189
MfeI CAATTG 2 cut(s) 3213, 5262
MflI RGATCY 5 cut(s) 2953, 3637, 4345, 4475, 5290
MhlI GDGCHC 4 cut(s) 2413, 3019, 3722, 4871
MlsI TGGCCA 1 cut(s) 4807
MluNI TGGCCA 1 cut(s) 4807
MmeI TCCRAC 2 cut(s) 4632, 5329
Mox20I TGGCCA 1 cut(s) 4807
Mph1103I ATGCAT 2 cut(s) 2214, 3406
MroXI GAANNNNTTC 3 cut(s) 1109, 3693, 5387
MscI TGGCCA 1 cut(s) 4807
MslI CAYNNNNRTG 8 cut(s) 89, 660, 1932, 2579, 3080, 4251, 4367, 5026
Msp20I TGGCCA 1 cut(s) 4807
MspA1I CMGCKG 6 cut(s) 1205, 1391, 3191, 3782, 3811, 4664
MspCI CTTAAG 2 cut(s) 1862, 3427
MspI CCGG 6 cut(s) 447, 513, 672, 1187, 3659, 4745
MspR9I CCNGG 5 cut(s) 3104, 3331, 3659, 4191, 4830
MunI CAATTG 2 cut(s) 3213, 5262
Mva1269I GAATGC 7 cut(s) 774, 1109, 2292, 2449, 4233, 5314, 5442
MvaI CCWGG 4 cut(s) 3104, 3331, 4191, 4830
MvnI CGCG 4 cut(s) 315, 323, 336, 374
NciI CCSGG 1 cut(s) 3659
NdeI CATATG 3 cut(s) 2752, 3790, 5464
NmeAIII GCCGAG 1 cut(s) 577
NmuCI GTSAC 8 cut(s) 375, 527, 643, 782, 1402, 1439, 4990, 5327
NsiI ATGCAT 2 cut(s) 2214, 3406
OliI CACNNNNGTG 1 cut(s) 4367
PaeI GCATGC 2 cut(s) 1931, 2673
PagI TCATGA 2 cut(s) 2296, 3466
PasI CCCWGGG 1 cut(s) 4829
PauI GCGCGC 1 cut(s) 313
PceI AGGCCT 2 cut(s) 3102, 3772
PciI ACATGT 6 cut(s) 655, 1196, 2275, 3038, 3115, 3733
PciSI GCTCTTC 6 cut(s) 303, 963, 1629, 2142, 2680, 3727
PcsI WCGNNNNNNNCGW 3 cut(s) 333, 389, 537
PctI GAATGC 7 cut(s) 774, 1109, 2292, 2449, 4233, 5314, 5442
PdmI GAANNNNTTC 3 cut(s) 1109, 3693, 5387
PflMI CCANNNNNTGG 4 cut(s) 1805, 2186, 2569, 5092
PmaCI CACGTG 1 cut(s) 328
PmlI CACGTG 1 cut(s) 328
Ppu21I YACGTR 3 cut(s) 328, 2552, 3311
PpuMI RGGWCCY 1 cut(s) 1243
PscI ACATGT 6 cut(s) 655, 1196, 2275, 3038, 3115, 3733
Psp124BI GAGCTC 1 cut(s) 3722
Psp1406I AACGTT 1 cut(s) 235
Psp5II RGGWCCY 1 cut(s) 1243
Psp6I CCWGG 4 cut(s) 3102, 3329, 4189, 4828
PspCI CACGTG 1 cut(s) 328
PspFI CCCAGC 2 cut(s) 3675, 5107
PspGI CCWGG 4 cut(s) 3102, 3329, 4189, 4828
PspPPI RGGWCCY 1 cut(s) 1243
PstNI CAGNNNCTG 5 cut(s) 2069, 4841, 5114, 5431, 5564
PsuI RGATCY 5 cut(s) 2953, 3637, 4345, 4475, 5290
PteI GCGCGC 1 cut(s) 313
PvuII CAGCTG 2 cut(s) 1205, 4664
RseI CAYNNNNRTG 8 cut(s) 89, 660, 1932, 2579, 3080, 4251, 4367, 5026
SacI GAGCTC 1 cut(s) 3722
SalI GTCGAC 2 cut(s) 539, 5457
SapI GCTCTTC 6 cut(s) 303, 963, 1629, 2142, 2680, 3727
ScaI AGTACT 1 cut(s) 3341
ScrFI CCNGG 5 cut(s) 3104, 3331, 3659, 4191, 4830
SduI GDGCHC 4 cut(s) 2413, 3019, 3722, 4871
SexAI ACCWGGT 1 cut(s) 4189
SfcI CTRYAG 7 cut(s) 754, 1962, 2835, 3408, 4014, 4137, 4314
SinI GGWCC 9 cut(s) 406, 1243, 2792, 3044, 3296, 4121, 4388, 4458, 5285
SmiMI CAYNNNNRTG 8 cut(s) 89, 660, 1932, 2579, 3080, 4251, 4367, 5026
SmlI CTYRAG 7 cut(s) 266, 416, 501, 572, 1862, 3335, 3427
SmoI CTYRAG 7 cut(s) 266, 416, 501, 572, 1862, 3335, 3427
SnaBI TACGTA 1 cut(s) 3311
SpeI ACTAGT 1 cut(s) 4994
SphI GCATGC 2 cut(s) 1931, 2673
SseBI AGGCCT 2 cut(s) 3102, 3772
SspI AATATT 2 cut(s) 1897, 4822
SstI GAGCTC 1 cut(s) 3722
StuI AGGCCT 2 cut(s) 3102, 3772
StyD4I CCNGG 5 cut(s) 3102, 3329, 3657, 4189, 4828
StyI CCWWGG 7 cut(s) 285, 1031, 3351, 3979, 4554, 4802, 5145
TatI WGTACW 3 cut(s) 2653, 3112, 3339
TauI GCSGC 5 cut(s) 334, 3194, 3491, 3799, 3814
TseFI GTSAC 8 cut(s) 375, 527, 643, 782, 1402, 1439, 4990, 5327
Tsp45I GTSAC 8 cut(s) 375, 527, 643, 782, 1402, 1439, 4990, 5327
TspGWI ACGGA 2 cut(s) 155, 4012
Van91I CCANNNNNTGG 4 cut(s) 1805, 2186, 2569, 5092
Vha464I CTTAAG 2 cut(s) 1862, 3427
VpaK11BI GGWCC 9 cut(s) 406, 1243, 2792, 3044, 3296, 4121, 4388, 4458, 5285
XagI CCTNNNNNAGG 1 cut(s) 927
XapI RAATTY 9 cut(s) 454, 1311, 2426, 3276, 3706, 3989, 4441, 5096, 5157
XmiI GTMKAC 4 cut(s) 540, 3858, 4046, 5458
XmnI GAANNNNTTC 3 cut(s) 1109, 3693, 5387
ZraI GACGTC 1 cut(s) 256
ZrmI AGTACT 1 cut(s) 3341
Zsp2I ATGCAT 2 cut(s) 2214, 3406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.