Rh1BG281300

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004331
Physical Location & Seq
Reverse (-)
41782246 .. 41786611
4366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG281300.1

Sequence Viewer

Length: 1038 bp
ATGGGTTACCAAAACGCACTATTTGCATCTCTTTACATTTTGCTACTTTCATTTGGAGCTTGTTTTGCTTCTTCTATTCAAGATCAAGTGAGGGATAGAATAACACAGTTGCCAGGGCAGCCAGCCAATGTGGGGTTTCGTCAGTATTCTGGTTATGTCACTGCCAATGAGCAAGCTGGGAGAGCATTGTTTTACTGGTTGGTTGAGTCCCCAGCGCATCGTGAACCGGAGTCCAGACCGCTAGTGTTGTGGCTCAACGGTGGTCCAGGTTGTTCTTCTGTTGCTTATGGAGCAGCTGAAGAGATTGGACCTTTTCATATTAGACCTGATGGAGAGACCCTTTACATGAATCCATATGCTTGGAACAATTTGGCAAATTTGCTTTTCCTTGAATCTCCAGCTGGTGTTGGTTTTTCATACACGAATACGTCTTCAGATTTGTATACAGCTGGAGACCAGAGAACAGCTGAAGATGCGTATACATTTCTGGTCAATTGGTTTGAAAGGTTTCCTCAGTACAAGCACAGAGATTTCTACATTGCTGGAGAAAGTTATGCAGGTCATTATGTTCCTCAGCTGTCTCAATTGGTTTATGAGAGAAATAAGGGAATTCAGAATCCAGATATCAATTTTAAGGGGTTTATGGTAGGAAATGCTGTAACTGATGATTACCATGATTTTGTTGGCACATTCGAATACTGGTGGACACATGGTTTAATTTCTGATTCCACCTATCGTGAGCTGCGAGTCAAGTGTGACTTGGATGTCTCTCAGCATCCATCACTGGCTTGCATGGAGGCCCTCAAAGTAGCAGATTCAGAGCAGGGAAACATTGACCCATACAGCATTTACACCCGTCCTTGTAGTAGTACTGCAGCACTAAAGCGCAACTTGAGGGGTCATTATCCATGGATGTCCAGAGCATATGATCCATGCACTGAGAGGTATTCTGAAAAGTATTTTAACCATCCAGAAGTCCAAAAGGCACTCCATGCAAATGTAACTGGAGTTTCGTACCCATGGAGAACATGCAGGTAG

Protein Analysis

345

Amino Acids

39.25

Weight (kDa)

5.69

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 38 - 344 2.3e-112 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 764
Acc36I ACCTGC 2 cut(s) 548, 1023
AccI GTMKAC 2 cut(s) 443, 479
AciI CCGC 1 cut(s) 239
AclWI GGATC 1 cut(s) 923
AcsI RAATTY 2 cut(s) 376, 609
AcuI CTGAAG 3 cut(s) 318, 417, 489
AfaI GTAC 3 cut(s) 518, 871, 1016
AfiI CCNNNNNNNGG 1 cut(s) 132
AgsI TTSAA 3 cut(s) 80, 392, 503
AjnI CCWGG 2 cut(s) 112, 265
AluBI AGCT 8 cut(s) 59, 176, 296, 401, 449, 467, 577, 742
AluI AGCT 8 cut(s) 59, 176, 296, 401, 449, 467, 577, 742
Alw26I GTCTC 4 cut(s) 329, 447, 585, 772
AlwI GGATC 1 cut(s) 923
AoxI GGCC 1 cut(s) 798
ApeKI GCWGC 4 cut(s) 118, 293, 742, 875
ApoI RAATTY 2 cut(s) 376, 609
Asp700I GAANNNNTTC 1 cut(s) 507
AspLEI GCGC 2 cut(s) 217, 888
AspS9I GGNCC 3 cut(s) 263, 308, 799
AsuII TTCGAA 1 cut(s) 693
AvaII GGWCC 2 cut(s) 263, 308
BbsI GAAGAC 1 cut(s) 423
BbvCI CCTCAGC 1 cut(s) 573
BbvI GCAGC 4 cut(s) 130, 305, 729, 887
BccI CCATC 3 cut(s) 323, 787, 975
BciT130I CCWGG 2 cut(s) 114, 267
BcoDI GTCTC 4 cut(s) 329, 447, 585, 772
BfaI CTAG 1 cut(s) 242
BfmI CTRYAG 1 cut(s) 873
BfuAI ACCTGC 2 cut(s) 548, 1023
BisI GCNGC 4 cut(s) 119, 294, 743, 876
BlsI GCNGC 4 cut(s) 120, 295, 744, 877
BmcAI AGTACT 1 cut(s) 871
Bme1390I CCNGG 2 cut(s) 114, 267
Bme18I GGWCC 2 cut(s) 263, 308
BmgT120I GGNCC 3 cut(s) 263, 308, 799
BmrFI CCNGG 2 cut(s) 114, 267
BmsI GCATC 4 cut(s) 35, 226, 463, 784
BpiI GAAGAC 1 cut(s) 423
BpmI CTGGAG 4 cut(s) 381, 471, 564, 1026
Bpu10I CCTNAGC 1 cut(s) 573
Bpu14I TTCGAA 1 cut(s) 693
BpuEI CTTGAG 1 cut(s) 913
BsaI GGTCTC 2 cut(s) 329, 447
BsaJI CCNNGG 3 cut(s) 113, 908, 1019
BsaWI WCCGGW 1 cut(s) 226
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse1I ACTGG 4 cut(s) 200, 704, 789, 1009
Bse3DI GCAATG 1 cut(s) 537
BseBI CCWGG 2 cut(s) 114, 267
BseDI CCNNGG 3 cut(s) 113, 908, 1019
BseGI GGATG 4 cut(s) 769, 775, 918, 967
BseLI CCNNNNNNNGG 1 cut(s) 132
BseMI GCAATG 1 cut(s) 537
BseMII CTCAG 4 cut(s) 527, 587, 785, 930
BseNI ACTGG 4 cut(s) 200, 704, 789, 1009
BseXI GCAGC 4 cut(s) 130, 305, 729, 887
BseYI CCCAGC 2 cut(s) 176, 211
BshFI GGCC 1 cut(s) 800
BsiSI CCGG 1 cut(s) 227
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 1 cut(s) 132
BsmAI GTCTC 4 cut(s) 329, 447, 585, 772
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 1 cut(s) 800
Bso31I GGTCTC 2 cut(s) 329, 447
Bsp119I TTCGAA 1 cut(s) 693
Bsp143I GATC 2 cut(s) 82, 928
Bsp19I CCATGG 2 cut(s) 908, 1019
BspACI CCGC 1 cut(s) 239
BspANI GGCC 1 cut(s) 800
BspCNI CTCAG 4 cut(s) 526, 586, 784, 931
BspMAI CTGCAG 1 cut(s) 877
BspMI ACCTGC 2 cut(s) 548, 1023
BspPI GGATC 1 cut(s) 923
BspT104I TTCGAA 1 cut(s) 693
BspTNI GGTCTC 2 cut(s) 329, 447
BsrDI GCAATG 1 cut(s) 537
BsrI ACTGG 4 cut(s) 200, 704, 789, 1009
BssECI CCNNGG 3 cut(s) 113, 908, 1019
BssMI GATC 2 cut(s) 82, 928
BssNAI GTATAC 2 cut(s) 444, 480
BssT1I CCWWGG 2 cut(s) 908, 1019
Bst1107I GTATAC 2 cut(s) 444, 480
Bst2UI CCWGG 2 cut(s) 114, 267
Bst4CI ACNGT 2 cut(s) 108, 260
Bst6I CTCTTC 1 cut(s) 294
BstAPI GCANNNNNTGC 2 cut(s) 23, 992
BstBI TTCGAA 1 cut(s) 693
BstC8I GCNNGC 3 cut(s) 123, 174, 790
BstDEI CTNAG 4 cut(s) 513, 573, 771, 939
BstDSI CCRYGG 2 cut(s) 908, 1019
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 4 cut(s) 769, 775, 918, 967
BstHHI GCGC 2 cut(s) 217, 888
BstKTI GATC 2 cut(s) 85, 931
BstMAI GTCTC 4 cut(s) 329, 447, 585, 772
BstMBI GATC 2 cut(s) 82, 928
BstMWI GCNNNNNNNGC 7 cut(s) 23, 65, 118, 182, 290, 473, 992
BstNI CCWGG 2 cut(s) 114, 267
BstNSI RCATGY 1 cut(s) 1032
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 2 cut(s) 112, 265
BstSFI CTRYAG 1 cut(s) 873
BstV1I GCAGC 4 cut(s) 130, 305, 729, 887
BstV2I GAAGAC 1 cut(s) 423
BstXI CCANNNNNNTGG 1 cut(s) 360
BstZ17I GTATAC 2 cut(s) 444, 480
BsuRI GGCC 1 cut(s) 800
BtgI CCRYGG 2 cut(s) 908, 1019
BtsCI GGATG 4 cut(s) 769, 775, 918, 967
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 3 cut(s) 159, 782, 936
BveI ACCTGC 2 cut(s) 548, 1023
Cac8I GCNNGC 3 cut(s) 123, 174, 790
CfoI GCGC 2 cut(s) 217, 888
Cfr13I GGNCC 3 cut(s) 263, 308, 799
Csp6I GTAC 3 cut(s) 517, 870, 1015
CviAII CATG 9 cut(s) 346, 674, 710, 793, 909, 933, 992, 1020, 1029
CviQI GTAC 3 cut(s) 517, 870, 1015
DdeI CTNAG 4 cut(s) 513, 573, 771, 939
DpnI GATC 2 cut(s) 84, 930
DpnII GATC 2 cut(s) 82, 928
DrdI GACNNNNNNGTC 1 cut(s) 764
DseDI GACNNNNNNGTC 1 cut(s) 764
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
Eco130I CCWWGG 2 cut(s) 908, 1019
Eco31I GGTCTC 2 cut(s) 329, 447
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 2 cut(s) 263, 308
Eco57I CTGAAG 3 cut(s) 318, 417, 489
Eco91I GGTNACC 1 cut(s) 5
EcoO109I RGGNCCY 1 cut(s) 799
EcoO65I GGTNACC 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 609
EcoRII CCWGG 2 cut(s) 112, 265
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 2 cut(s) 908, 1019
ErhI CCWWGG 2 cut(s) 908, 1019
FaeI CATG 9 cut(s) 349, 677, 713, 796, 912, 936, 995, 1023, 1032
FalI AAGNNNNNCTT 4 cut(s) 743, 775, 875, 907
FaqI GGGAC 1 cut(s) 193
FatI CATG 9 cut(s) 345, 673, 709, 792, 908, 932, 991, 1019, 1028
FauNDI CATATG 2 cut(s) 355, 925
FblI GTMKAC 2 cut(s) 443, 479
Fnu4HI GCNGC 4 cut(s) 119, 294, 743, 876
FokI GGATG 4 cut(s) 762, 776, 925, 954
Fsp4HI GCNGC 4 cut(s) 119, 294, 743, 876
FspBI CTAG 1 cut(s) 242
GlaI GCGC 2 cut(s) 216, 887
GluI GCNGC 4 cut(s) 119, 294, 743, 876
GsaI CCCAGC 2 cut(s) 180, 215
GsuI CTGGAG 4 cut(s) 381, 471, 564, 1026
HaeIII GGCC 1 cut(s) 800
HapII CCGG 1 cut(s) 227
HhaI GCGC 2 cut(s) 217, 888
Hin1II CATG 9 cut(s) 349, 677, 713, 796, 912, 936, 995, 1023, 1032
Hin6I GCGC 2 cut(s) 215, 886
HinP1I GCGC 2 cut(s) 215, 886
HinfI GANTC 8 cut(s) 206, 230, 349, 392, 616, 725, 747, 815
HpaII CCGG 1 cut(s) 227
Hpy166II GTNNAC 4 cut(s) 224, 444, 480, 705
Hpy188I TCNGA 5 cut(s) 436, 615, 724, 820, 952
Hpy188III TCNNGA 7 cut(s) 80, 221, 234, 620, 737, 918, 971
Hpy8I GTNNAC 4 cut(s) 224, 444, 480, 705
HpyCH4III ACNGT 2 cut(s) 108, 260
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 7 cut(s) 26, 557, 792, 875, 936, 995, 1032
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 65, 118, 182, 290, 473, 992
HpyF3I CTNAG 4 cut(s) 513, 573, 771, 939
HpySE526I ACGT 1 cut(s) 428
Hsp92II CATG 9 cut(s) 349, 677, 713, 796, 912, 936, 995, 1023, 1032
HspAI GCGC 2 cut(s) 215, 886
Kzo9I GATC 2 cut(s) 82, 928
LmnI GCTCC 2 cut(s) 56, 290
Lsp1109I GCAGC 4 cut(s) 130, 305, 729, 887
LweI GCATC 4 cut(s) 35, 226, 463, 784
MaeI CTAG 1 cut(s) 242
MaeII ACGT 1 cut(s) 428
MaeIII GTNAC 5 cut(s) 5, 157, 658, 755, 1000
MalI GATC 2 cut(s) 84, 930
MboI GATC 2 cut(s) 82, 928
MboII GAAGA 5 cut(s) 63, 267, 311, 423, 482
MfeI CAATTG 2 cut(s) 493, 584
MluCI AATT 7 cut(s) 367, 376, 493, 584, 609, 628, 717
MlyI GAGTC 3 cut(s) 215, 239, 756
MnlI CCTC 7 cut(s) 84, 522, 582, 790, 812, 888, 936
MroXI GAANNNNTTC 1 cut(s) 507
MseI TTAA 3 cut(s) 633, 716, 963
MslI CAYNNNNRTG 1 cut(s) 996
MspA1I CMGCKG 5 cut(s) 296, 401, 449, 467, 577
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 2 cut(s) 114, 267
MunI CAATTG 2 cut(s) 493, 584
MvaI CCWGG 2 cut(s) 114, 267
MwoI GCNNNNNNNGC 7 cut(s) 23, 65, 118, 182, 290, 473, 992
NcoI CCATGG 2 cut(s) 908, 1019
NdeI CATATG 2 cut(s) 355, 925
NdeII GATC 2 cut(s) 82, 928
NlaIII CATG 9 cut(s) 349, 677, 713, 796, 912, 936, 995, 1023, 1032
NmuCI GTSAC 2 cut(s) 157, 755
NspI RCATGY 1 cut(s) 1032
NspV TTCGAA 1 cut(s) 693
PcsI WCGNNNNNNNCGW 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 507
PfeI GAWTC 5 cut(s) 349, 392, 616, 725, 815
PkrI GCNGC 4 cut(s) 120, 295, 744, 877
PleI GAGTC 3 cut(s) 214, 238, 755
PpsI GAGTC 3 cut(s) 214, 238, 755
Psp6I CCWGG 2 cut(s) 112, 265
PspEI GGTNACC 1 cut(s) 5
PspFI CCCAGC 2 cut(s) 176, 211
PspGI CCWGG 2 cut(s) 112, 265
PspPI GGNCC 3 cut(s) 263, 308, 799
PstI CTGCAG 1 cut(s) 877
PvuII CAGCTG 5 cut(s) 296, 401, 449, 467, 577
RsaI GTAC 3 cut(s) 518, 871, 1016
RsaNI GTAC 3 cut(s) 517, 870, 1015
RseI CAYNNNNRTG 1 cut(s) 996
SaqAI TTAA 3 cut(s) 633, 716, 963
SatI GCNGC 4 cut(s) 119, 294, 743, 876
Sau3AI GATC 2 cut(s) 82, 928
Sau96I GGNCC 3 cut(s) 263, 308, 799
ScaI AGTACT 1 cut(s) 871
SchI GAGTC 3 cut(s) 215, 239, 756
ScrFI CCNGG 2 cut(s) 114, 267
SfaNI GCATC 4 cut(s) 35, 226, 463, 784
SfcI CTRYAG 1 cut(s) 873
SfuI TTCGAA 1 cut(s) 693
SinI GGWCC 2 cut(s) 263, 308
SmiMI CAYNNNNRTG 1 cut(s) 996
SmlI CTYRAG 1 cut(s) 892
SmoI CTYRAG 1 cut(s) 892
Sse9I AATT 7 cut(s) 367, 376, 493, 584, 609, 628, 717
SsiI CCGC 1 cut(s) 239
SspMI CTAG 1 cut(s) 242
StyD4I CCNGG 2 cut(s) 112, 265
StyI CCWWGG 2 cut(s) 908, 1019
TaaI ACNGT 2 cut(s) 108, 260
TaiI ACGT 1 cut(s) 431
TaqI TCGA 1 cut(s) 693
TasI AATT 7 cut(s) 367, 376, 493, 584, 609, 628, 717
TatI WGTACW 2 cut(s) 516, 869
TfiI GAWTC 5 cut(s) 349, 392, 616, 725, 815
Tru1I TTAA 3 cut(s) 633, 716, 963
Tru9I TTAA 3 cut(s) 633, 716, 963
TscAI CASTG 3 cut(s) 166, 789, 943
TseFI GTSAC 2 cut(s) 157, 755
TseI GCWGC 4 cut(s) 118, 293, 742, 875
Tsp45I GTSAC 2 cut(s) 157, 755
TspDTI ATGAA 4 cut(s) 39, 305, 362, 405
TspRI CASTG 3 cut(s) 166, 789, 943
VpaK11BI GGWCC 2 cut(s) 263, 308
XapI RAATTY 2 cut(s) 376, 609
XceI RCATGY 1 cut(s) 1032
XcmI CCANNNNNNNNNTGG 1 cut(s) 680
XmiI GTMKAC 2 cut(s) 443, 479
XmnI GAANNNNTTC 1 cut(s) 507
XspI CTAG 1 cut(s) 242
ZrmI AGTACT 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.