RchiOBHm_Chr1g0363831

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004331
Physical Location & Seq
Reverse (-)
55145890 .. 55149730
3841 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58854

Sequence Viewer

Length: 1377 bp
ATGGGTTACCAAAGCCCACTATTTGCATCTCTTTGCATTTTGCTACTTTCATTTGGAGCTAGTTTTGCTTCTTCTATTCAAGATCAAGTGAGGGATAGAATAACACAGTTGCCAGGGCAGCCAGCCAATGTGGGGTTTCGTCAGTATTCTGGTTATGTCACTGCCAATGAGCAAGCTGGGAGAGCATTGTTTTACTGGTTGGTTGAGTCCCCAGCGCATCGTGAACCGGAGTCCAGACCGCTAGTGTTGTGGCTCGCTGGTGGTCCAGGTTGTTCTTCTGTTGGTCATGGATCAGCTGAAGAGATTGGACCTTTTCATATTAGACCTGATGGAGAGACCCTTTACATGAATCCGTATGCTTGGAACAATTTGGCAAATTTGCTTTTCCTTGAATCTCCAGCTGGTGTTGGTTTTTCATACACTAATACGTCTTCAGATTTGTATACATCTGGAGACCAGAGAACAGCTGAAGATGCGTATACATTTCTGGTCAATTGGTTTGAAAGGTTTCCTCAGTACAAGCACAGAGATTTCTACATTGCTGGAGAAAGTTATGCAGGTCATTATGTTCCTCAGCTGTCTCAATTGGTTTATGAGAGAAATAAGGGAATTCAGAATCCAGATATCAATTTTAAAGGGTTTATGGTAGGAAATGCTGTAACTGATGATTACCATGATCTTGTTGGCACATTCGAACACTGGTGGACACATGGTTTAATTTCTGATTCCACCTATCGTGAGCTGCGAGTCAAGTGTTACAATGTTTCCATTCTGCATCTATCACTGGCTTGCTCGGAGGCCCTCAAAGTAGCAGATTCAGAGCAGGGAAACATTGACCCATACAGCATTTACACCCGTCCTTGTAATAGTACTGCAGCACTAAAGCGCAACTTGAGGGGTCATTATCCATGGATGTCCAGAGCATATGATCCATGCACTGTGTTGTATTCTGAAAAGTATTTTAACCATCCAGAAGTCCAAAAGGCACTCCATGCAAATGTAACTGGAGTTTCGTACCGTTGGACAACATGCAGTGATGTTATTGCAGACAACTGGTCGGATTCTCCACTGTCTGTGCTTCCAATTTATCAAGAGCTCATTGCTGCGGGTCTCAAGATATGGGTATACAGTGGAGATACTGATTCAGTGGTTCCTGTGACTGCCACGCGTTACTCCATAGACGCCCTTAAGTTACCGACCATCACCAATTGGTTCCCATGGTATGATACTGGCAAGGTTGGTGGCTGGAGCCAAATATACAAGGGGCTGACATTAGTCACCATTACCGGAGCTGGACATCAGGTCCCACTCCTTCGACCTCGAGAAGCTTTCATTGTTTTCAGATCATTTTTGGAGAACAAGCCCATGCCTAGTTAA

Protein Analysis

458

Amino Acids

51.52

Weight (kDa)

5.9

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 38 - 453 5.2e-145 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 548
AccI GTMKAC 3 cut(s) 443, 479, 1125
AccII CGCG 1 cut(s) 1168
AciI CCGC 2 cut(s) 239, 1106
AclWI GGATC 2 cut(s) 298, 923
AcsI RAATTY 2 cut(s) 376, 609
AcuI CTGAAG 3 cut(s) 318, 417, 489
AcyI GRCGYC 1 cut(s) 1182
AfaI GTAC 3 cut(s) 518, 871, 1016
AfiI CCNNNNNNNGG 1 cut(s) 132
AflII CTTAAG 1 cut(s) 1187
AflIII ACRYGT 1 cut(s) 1166
AgsI TTSAA 3 cut(s) 80, 392, 503
AhdI GACNNNNNGTC 2 cut(s) 1054, 1301
AjnI CCWGG 2 cut(s) 112, 265
Alw21I GWGCWC 1 cut(s) 1098
Alw26I GTCTC 4 cut(s) 329, 447, 585, 1115
AlwI GGATC 2 cut(s) 298, 923
Ama87I CYCGRG 1 cut(s) 1320
AoxI GGCC 1 cut(s) 798
ApeKI GCWGC 4 cut(s) 118, 742, 875, 1103
ApoI RAATTY 2 cut(s) 376, 609
Asp700I GAANNNNTTC 1 cut(s) 507
AspLEI GCGC 2 cut(s) 217, 888
AspS9I GGNCC 4 cut(s) 263, 308, 799, 1303
AsuHPI GGTGA 2 cut(s) 1195, 1270
AsuII TTCGAA 1 cut(s) 693
AvaI CYCGRG 1 cut(s) 1320
AvaII GGWCC 3 cut(s) 263, 308, 1303
BanII GRGCYC 1 cut(s) 1098
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 1098
BbvCI CCTCAGC 1 cut(s) 573
BbvI GCAGC 4 cut(s) 130, 729, 887, 1090
BccI CCATC 3 cut(s) 323, 975, 1208
BciT130I CCWGG 2 cut(s) 114, 267
BcoDI GTCTC 4 cut(s) 329, 447, 585, 1115
BfaI CTAG 3 cut(s) 60, 242, 1371
BfmI CTRYAG 1 cut(s) 873
BfrI CTTAAG 1 cut(s) 1187
BfuAI ACCTGC 1 cut(s) 548
BisI GCNGC 4 cut(s) 119, 743, 876, 1104
BlsI GCNGC 4 cut(s) 120, 744, 877, 1105
BmcAI AGTACT 1 cut(s) 871
Bme1390I CCNGG 2 cut(s) 114, 267
Bme18I GGWCC 3 cut(s) 263, 308, 1303
BmeRI GACNNNNNGTC 2 cut(s) 1054, 1301
BmeT110I CYCGRG 1 cut(s) 1320
BmgT120I GGNCC 4 cut(s) 263, 308, 799, 1303
BmiI GGNNCC 4 cut(s) 1152, 1214, 1250, 1305
BmrFI CCNGG 2 cut(s) 114, 267
BmsI GCATC 4 cut(s) 35, 226, 463, 784
BoxI GACNNNNGTC 1 cut(s) 1274
BpiI GAAGAC 1 cut(s) 423
BpmI CTGGAG 5 cut(s) 381, 471, 564, 1026, 1267
Bpu10I CCTNAGC 1 cut(s) 573
Bpu14I TTCGAA 1 cut(s) 693
BpuEI CTTGAG 2 cut(s) 913, 1097
BsaHI GRCGYC 1 cut(s) 1182
BsaI GGTCTC 3 cut(s) 329, 447, 1115
BsaJI CCNNGG 3 cut(s) 113, 908, 1217
BsaWI WCCGGW 2 cut(s) 226, 1286
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse1I ACTGG 6 cut(s) 200, 704, 789, 1009, 1058, 1234
Bse3DI GCAATG 2 cut(s) 537, 1098
BseBI CCWGG 2 cut(s) 114, 267
BseDI CCNNGG 3 cut(s) 113, 908, 1217
BseGI GGATG 2 cut(s) 918, 967
BseLI CCNNNNNNNGG 1 cut(s) 132
BseMI GCAATG 2 cut(s) 537, 1098
BseMII CTCAG 2 cut(s) 527, 587
BseNI ACTGG 6 cut(s) 200, 704, 789, 1009, 1058, 1234
BseXI GCAGC 4 cut(s) 130, 729, 887, 1090
BseYI CCCAGC 2 cut(s) 176, 211
Bsh1236I CGCG 1 cut(s) 1168
BshFI GGCC 1 cut(s) 800
BsiHKAI GWGCWC 1 cut(s) 1098
BsiHKCI CYCGRG 1 cut(s) 1320
BsiSI CCGG 2 cut(s) 227, 1287
BslFI GGGAC 2 cut(s) 193, 1289
BslI CCNNNNNNNGG 1 cut(s) 132
BsmAI GTCTC 4 cut(s) 329, 447, 585, 1115
BsmFI GGGAC 2 cut(s) 193, 1289
BsnI GGCC 1 cut(s) 800
Bso31I GGTCTC 3 cut(s) 329, 447, 1115
BsoBI CYCGRG 1 cut(s) 1320
Bsp119I TTCGAA 1 cut(s) 693
Bsp1286I GDGCHC 1 cut(s) 1098
Bsp143I GATC 5 cut(s) 82, 290, 676, 928, 1343
Bsp19I CCATGG 2 cut(s) 908, 1217
BspACI CCGC 2 cut(s) 239, 1106
BspANI GGCC 1 cut(s) 800
BspCNI CTCAG 2 cut(s) 526, 586
BspFNI CGCG 1 cut(s) 1168
BspLI GGNNCC 4 cut(s) 1152, 1214, 1250, 1305
BspMAI CTGCAG 1 cut(s) 877
BspMI ACCTGC 1 cut(s) 548
BspPI GGATC 2 cut(s) 298, 923
BspT104I TTCGAA 1 cut(s) 693
BspTI CTTAAG 1 cut(s) 1187
BspTNI GGTCTC 3 cut(s) 329, 447, 1115
BsrDI GCAATG 2 cut(s) 537, 1098
BsrI ACTGG 6 cut(s) 200, 704, 789, 1009, 1058, 1234
BssECI CCNNGG 3 cut(s) 113, 908, 1217
BssMI GATC 5 cut(s) 82, 290, 676, 928, 1343
BssNAI GTATAC 3 cut(s) 444, 480, 1126
BssNI GRCGYC 1 cut(s) 1182
BssT1I CCWWGG 2 cut(s) 908, 1217
Bst1107I GTATAC 3 cut(s) 444, 480, 1126
Bst2UI CCWGG 2 cut(s) 114, 267
Bst4CI ACNGT 5 cut(s) 108, 940, 1019, 1071, 1130
Bst6I CTCTTC 1 cut(s) 294
BstACI GRCGYC 1 cut(s) 1182
BstAFI CTTAAG 1 cut(s) 1187
BstAPI GCANNNNNTGC 1 cut(s) 992
BstBI TTCGAA 1 cut(s) 693
BstC8I GCNNGC 4 cut(s) 123, 174, 255, 790
BstDEI CTNAG 2 cut(s) 513, 573
BstDSI CCRYGG 2 cut(s) 908, 1217
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 2 cut(s) 918, 967
BstFNI CGCG 1 cut(s) 1168
BstHHI GCGC 2 cut(s) 217, 888
BstKTI GATC 5 cut(s) 85, 293, 679, 931, 1346
BstMAI GTCTC 4 cut(s) 329, 447, 585, 1115
BstMBI GATC 5 cut(s) 82, 290, 676, 928, 1343
BstMWI GCNNNNNNNGC 5 cut(s) 65, 118, 182, 473, 992
BstNI CCWGG 2 cut(s) 114, 267
BstNSI RCATGY 1 cut(s) 1032
BstPAI GACNNNNGTC 1 cut(s) 1274
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 2 cut(s) 112, 265
BstSFI CTRYAG 1 cut(s) 873
BstUI CGCG 1 cut(s) 1168
BstV1I GCAGC 4 cut(s) 130, 729, 887, 1090
BstV2I GAAGAC 1 cut(s) 423
BstZ17I GTATAC 3 cut(s) 444, 480, 1126
BsuRI GGCC 1 cut(s) 800
BtgI CCRYGG 2 cut(s) 908, 1217
BtsCI GGATG 2 cut(s) 918, 967
BtsI GCAGTG 2 cut(s) 159, 1039
BtsIMutI CAGTG 8 cut(s) 159, 697, 782, 936, 1039, 1067, 1135, 1152
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 4 cut(s) 123, 174, 255, 790
CfoI GCGC 2 cut(s) 217, 888
Cfr13I GGNCC 4 cut(s) 263, 308, 799, 1303
CseI GACGC 1 cut(s) 1190
Csp6I GTAC 3 cut(s) 517, 870, 1015
CspCI CAANNNNNGTGG 2 cut(s) 1222, 1257
CviQI GTAC 3 cut(s) 517, 870, 1015
DdeI CTNAG 2 cut(s) 513, 573
DpnI GATC 5 cut(s) 84, 292, 678, 930, 1345
DpnII GATC 5 cut(s) 82, 290, 676, 928, 1343
DraI TTTAAA 1 cut(s) 634
DriI GACNNNNNGTC 2 cut(s) 1054, 1301
Eam1104I CTCTTC 1 cut(s) 294
Eam1105I GACNNNNNGTC 2 cut(s) 1054, 1301
EarI CTCTTC 1 cut(s) 294
Ecl136II GAGCTC 1 cut(s) 1096
Eco130I CCWWGG 2 cut(s) 908, 1217
Eco24I GRGCYC 1 cut(s) 1098
Eco31I GGTCTC 3 cut(s) 329, 447, 1115
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 3 cut(s) 263, 308, 1303
Eco53kI GAGCTC 1 cut(s) 1096
Eco57I CTGAAG 3 cut(s) 318, 417, 489
Eco88I CYCGRG 1 cut(s) 1320
Eco91I GGTNACC 1 cut(s) 5
EcoICRI GAGCTC 1 cut(s) 1096
EcoO109I RGGNCCY 2 cut(s) 799, 1303
EcoO65I GGTNACC 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 609
EcoRII CCWGG 2 cut(s) 112, 265
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 2 cut(s) 908, 1217
EcoT38I GRGCYC 1 cut(s) 1098
ErhI CCWWGG 2 cut(s) 908, 1217
FalI AAGNNNNNCTT 2 cut(s) 875, 907
FaqI GGGAC 2 cut(s) 193, 1289
FauI CCCGC 1 cut(s) 1099
FauNDI CATATG 1 cut(s) 925
FblI GTMKAC 3 cut(s) 443, 479, 1125
Fnu4HI GCNGC 4 cut(s) 119, 743, 876, 1104
FokI GGATG 2 cut(s) 925, 954
FriOI GRGCYC 1 cut(s) 1098
Fsp4HI GCNGC 4 cut(s) 119, 743, 876, 1104
FspBI CTAG 3 cut(s) 60, 242, 1371
GlaI GCGC 2 cut(s) 216, 887
GluI GCNGC 4 cut(s) 119, 743, 876, 1104
GsaI CCCAGC 2 cut(s) 180, 215
GsuI CTGGAG 5 cut(s) 381, 471, 564, 1026, 1267
HaeIII GGCC 1 cut(s) 800
HapII CCGG 2 cut(s) 227, 1287
HgaI GACGC 1 cut(s) 1190
HhaI GCGC 2 cut(s) 217, 888
Hin1I GRCGYC 1 cut(s) 1182
Hin6I GCGC 2 cut(s) 215, 886
HinP1I GCGC 2 cut(s) 215, 886
HindIII AAGCTT 1 cut(s) 1326
HpaII CCGG 2 cut(s) 227, 1287
HphI GGTGA 2 cut(s) 1195, 1270
Hpy166II GTNNAC 5 cut(s) 224, 444, 480, 705, 1126
Hpy188I TCNGA 8 cut(s) 436, 615, 724, 796, 820, 952, 1060, 1343
Hpy8I GTNNAC 5 cut(s) 224, 444, 480, 705, 1126
HpyAV CCTTC 1 cut(s) 1322
HpyCH4III ACNGT 5 cut(s) 108, 940, 1019, 1071, 1130
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 9 cut(s) 26, 36, 557, 775, 875, 936, 995, 1032, 1046
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 118, 182, 473, 992
HpyF3I CTNAG 2 cut(s) 513, 573
HpySE526I ACGT 1 cut(s) 428
Hsp92I GRCGYC 1 cut(s) 1182
HspAI GCGC 2 cut(s) 215, 886
Kzo9I GATC 5 cut(s) 82, 290, 676, 928, 1343
LmnI GCTCC 3 cut(s) 56, 1248, 1289
Lsp1109I GCAGC 4 cut(s) 130, 729, 887, 1090
LweI GCATC 4 cut(s) 35, 226, 463, 784
MaeI CTAG 3 cut(s) 60, 242, 1371
MaeII ACGT 1 cut(s) 428
MaeIII GTNAC 9 cut(s) 5, 157, 658, 755, 1000, 1156, 1169, 1191, 1276
MalI GATC 5 cut(s) 84, 292, 678, 930, 1345
MboI GATC 5 cut(s) 82, 290, 676, 928, 1343
MboII GAAGA 5 cut(s) 63, 267, 311, 423, 482
MfeI CAATTG 3 cut(s) 493, 584, 1207
MhlI GDGCHC 1 cut(s) 1098
MluCI AATT 9 cut(s) 367, 376, 493, 584, 609, 628, 717, 1083, 1207
MluI ACGCGT 1 cut(s) 1166
MlyI GAGTC 3 cut(s) 215, 239, 756
MmeI TCCRAC 2 cut(s) 1001, 1038
MnlI CCTC 7 cut(s) 84, 522, 582, 790, 812, 888, 1329
MroXI GAANNNNTTC 1 cut(s) 507
MseI TTAA 5 cut(s) 633, 716, 963, 1188, 1375
MslI CAYNNNNRTG 1 cut(s) 996
MspA1I CMGCKG 4 cut(s) 296, 401, 467, 577
MspCI CTTAAG 1 cut(s) 1187
MspI CCGG 2 cut(s) 227, 1287
MspR9I CCNGG 2 cut(s) 114, 267
MunI CAATTG 3 cut(s) 493, 584, 1207
MvaI CCWGG 2 cut(s) 114, 267
MvnI CGCG 1 cut(s) 1168
MwoI GCNNNNNNNGC 5 cut(s) 65, 118, 182, 473, 992
NcoI CCATGG 2 cut(s) 908, 1217
NdeI CATATG 1 cut(s) 925
NdeII GATC 5 cut(s) 82, 290, 676, 928, 1343
NlaIV GGNNCC 4 cut(s) 1152, 1214, 1250, 1305
NmuCI GTSAC 3 cut(s) 157, 1156, 1276
NspI RCATGY 1 cut(s) 1032
NspV TTCGAA 1 cut(s) 693
PaeR7I CTCGAG 1 cut(s) 1320
PcsI WCGNNNNNNNCGW 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 507
PfeI GAWTC 7 cut(s) 349, 392, 616, 725, 815, 1061, 1142
PkrI GCNGC 4 cut(s) 120, 744, 877, 1105
PleI GAGTC 3 cut(s) 214, 238, 755
PpsI GAGTC 3 cut(s) 214, 238, 755
PpuMI RGGWCCY 1 cut(s) 1303
PshAI GACNNNNGTC 1 cut(s) 1274
Psp124BI GAGCTC 1 cut(s) 1098
Psp5II RGGWCCY 1 cut(s) 1303
Psp6I CCWGG 2 cut(s) 112, 265
PspEI GGTNACC 1 cut(s) 5
PspFI CCCAGC 2 cut(s) 176, 211
PspGI CCWGG 2 cut(s) 112, 265
PspN4I GGNNCC 4 cut(s) 1152, 1214, 1250, 1305
PspPI GGNCC 4 cut(s) 263, 308, 799, 1303
PspPPI RGGWCCY 1 cut(s) 1303
PstI CTGCAG 1 cut(s) 877
PvuII CAGCTG 4 cut(s) 296, 401, 467, 577
RsaI GTAC 3 cut(s) 518, 871, 1016
RsaNI GTAC 3 cut(s) 517, 870, 1015
RseI CAYNNNNRTG 1 cut(s) 996
SacI GAGCTC 1 cut(s) 1098
SaqAI TTAA 5 cut(s) 633, 716, 963, 1188, 1375
SatI GCNGC 4 cut(s) 119, 743, 876, 1104
Sau3AI GATC 5 cut(s) 82, 290, 676, 928, 1343
Sau96I GGNCC 4 cut(s) 263, 308, 799, 1303
ScaI AGTACT 1 cut(s) 871
SchI GAGTC 3 cut(s) 215, 239, 756
ScrFI CCNGG 2 cut(s) 114, 267
SduI GDGCHC 1 cut(s) 1098
SfaNI GCATC 4 cut(s) 35, 226, 463, 784
SfcI CTRYAG 1 cut(s) 873
Sfr274I CTCGAG 1 cut(s) 1320
SfuI TTCGAA 1 cut(s) 693
SinI GGWCC 3 cut(s) 263, 308, 1303
SlaI CTCGAG 1 cut(s) 1320
SmiMI CAYNNNNRTG 1 cut(s) 996
SmlI CTYRAG 4 cut(s) 892, 1112, 1187, 1320
SmoI CTYRAG 4 cut(s) 892, 1112, 1187, 1320
Sse9I AATT 9 cut(s) 367, 376, 493, 584, 609, 628, 717, 1083, 1207
SsiI CCGC 2 cut(s) 239, 1106
SspMI CTAG 3 cut(s) 60, 242, 1371
SstI GAGCTC 1 cut(s) 1098
StyD4I CCNGG 2 cut(s) 112, 265
StyI CCWWGG 2 cut(s) 908, 1217
TaaI ACNGT 5 cut(s) 108, 940, 1019, 1071, 1130
TaiI ACGT 1 cut(s) 431
TaqI TCGA 3 cut(s) 693, 1315, 1321
TasI AATT 9 cut(s) 367, 376, 493, 584, 609, 628, 717, 1083, 1207
TatI WGTACW 2 cut(s) 516, 869
TfiI GAWTC 7 cut(s) 349, 392, 616, 725, 815, 1061, 1142
Tru1I TTAA 5 cut(s) 633, 716, 963, 1188, 1375
Tru9I TTAA 5 cut(s) 633, 716, 963, 1188, 1375
TscAI CASTG 8 cut(s) 166, 704, 789, 943, 1039, 1074, 1135, 1152
TseFI GTSAC 3 cut(s) 157, 1156, 1276
TseI GCWGC 4 cut(s) 118, 742, 875, 1103
Tsp45I GTSAC 3 cut(s) 157, 1156, 1276
TspDTI ATGAA 5 cut(s) 39, 305, 362, 405, 1321
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 8 cut(s) 166, 704, 789, 943, 1039, 1074, 1135, 1152
Vha464I CTTAAG 1 cut(s) 1187
VpaK11BI GGWCC 3 cut(s) 263, 308, 1303
XapI RAATTY 2 cut(s) 376, 609
XceI RCATGY 1 cut(s) 1032
XcmI CCANNNNNNNNNTGG 1 cut(s) 680
XhoI CTCGAG 1 cut(s) 1320
XmiI GTMKAC 3 cut(s) 443, 479, 1125
XmnI GAANNNNTTC 1 cut(s) 507
XspI CTAG 3 cut(s) 60, 242, 1371
ZrmI AGTACT 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.