Rh1AG318000

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004331
Physical Location & Seq
Reverse (-)
55193460 .. 55196436
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG318000.1

Sequence Viewer

Length: 1380 bp
ATGGGTTACCAAAGCCCACTATTTGCATCTCTTTGCATTTTGCTACTTTCATTTGGAGCTTGTTTTGCTTCTTCTATTCAAGATCAATTGAGGGATAGAATAACACAGTTGCCAGGGCAGCCAGCCAATGTGGGGTTTCGTCAGTATTCTGGTTATGTCACTGCCAATGAGCAAGCTGGGAGAGCATTGTTTTACTGGTTGGTTGAGTCCCCAGCGCATCGTGAACCGGAGTCCAGACCGCTAGTGTTGTGGCTCAATGGTGGTCCAGGTTGTTCTTCTGTTGCATATGGAGCAGCTGAAGAGATTGGACCTTTTCATATTAGACCTGATGGAGAGACCCTTTACATGAATCCATATGCTTGGAACAAATTGGCAAATTTGCTTTTCCTTGAATCTCCAGCTGGTGTTGGTTTTTCATACACGAATATGTCTTCAGATTTGTATACAGCTGGAGACCAGAGAACAGCTGAAGATGCGTATACTTTTCTGGTCAATTGGTTTGAAAGGTTTCCTCAGTACAAGCACAGAGATTTCTACATTGCTGGAGAAAGTTATGCAGGTCATTATGTTCCTCAGCTGTCTCAATTGGTTTATGAGAGAAATAAGGGAATTCAGAATCCAGATATCAATTTTAAGGGGTTTATGGTAGGAAATGCTGTAACTGATGATTACCATGATTTTGTTGGTACATTCGAATACTGGTGGACACATGGTTTAATTTCTGATTCCACCTATCGTGAGCTGCGAGTCAAGTGTGACTTGGATGTCTCTCAGCATCCATCACTGGCTTGCATGGTGGCCCTCAAAGTAGCAGATTCAGAGCAGGGAAACATTGACCCATACAGCATTTACACCCGTCCTTGTAGTAGTACTGCAGCACTAAAGCGCAACTTGAGGGGTCATTATCCATGGATGTCCAGAGCATATGATCCATGCACTGAGAGGTATTCTGAAAAGTATTTTAACCATCCAGAAGTTCAAAAGGCACTCCATGCAAATGTAACTGGAGTTTTGTACCCGTGGAAAACATGCAGTGATATTGTTGGAAACTACTGGTCAGATTCTCCACGGTCTATGCTTCCAATTTATCAAGAGCTCATTGCTGCGGGTCTCAAGATATGGGTATACAGTGGAGATACTGATGCAGTGGTTCCTGTGACTGCCACACGTTACTCCATAGATGCCCTTAAGTTACCGACCATCACCAATTGGTCCCCATGGTATGATACTGGCAAGGTTGGTGGCTGGAGCCAAATATACAAGGGGCTGACATTAGTCACCATTACCGGAGCTGGACATGAGGTCCCACTCCATCGACCTCGAGAAGCTTTCATTGTTTTCAGATCATTTTTGGAGAACAAGCCCATGCGGCACGCCTAG

Protein Analysis

459

Amino Acids

51.94

Weight (kDa)

6.12

Isoelectric Point (pI)

44.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 38 - 453 1.4e-148 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 764
Acc36I ACCTGC 1 cut(s) 548
AccI GTMKAC 3 cut(s) 443, 479, 1125
AciI CCGC 3 cut(s) 239, 1106, 1369
AclWI GGATC 1 cut(s) 923
AcsI RAATTY 2 cut(s) 376, 609
AcuI CTGAAG 3 cut(s) 318, 417, 489
AfaI GTAC 4 cut(s) 518, 688, 871, 1016
AfiI CCNNNNNNNGG 1 cut(s) 132
AflII CTTAAG 1 cut(s) 1187
AflIII ACRYGT 1 cut(s) 1166
AgsI TTSAA 4 cut(s) 80, 392, 503, 980
AhdI GACNNNNNGTC 1 cut(s) 1301
AjnI CCWGG 2 cut(s) 112, 265
Alw21I GWGCWC 1 cut(s) 1098
Alw26I GTCTC 5 cut(s) 329, 447, 585, 772, 1115
AlwI GGATC 1 cut(s) 923
Ama87I CYCGRG 1 cut(s) 1320
AoxI GGCC 1 cut(s) 798
ApeKI GCWGC 5 cut(s) 118, 293, 742, 875, 1103
ApoI RAATTY 2 cut(s) 376, 609
Asp700I GAANNNNTTC 1 cut(s) 507
AspLEI GCGC 2 cut(s) 217, 888
AspS9I GGNCC 5 cut(s) 263, 308, 799, 1212, 1303
AsuHPI GGTGA 2 cut(s) 1195, 1270
AsuII TTCGAA 1 cut(s) 693
AvaI CYCGRG 1 cut(s) 1320
AvaII GGWCC 4 cut(s) 263, 308, 1212, 1303
BanII GRGCYC 1 cut(s) 1098
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 1098
BbvCI CCTCAGC 1 cut(s) 573
BbvI GCAGC 5 cut(s) 130, 305, 729, 887, 1090
BccI CCATC 5 cut(s) 323, 787, 975, 1208, 1320
BciT130I CCWGG 2 cut(s) 114, 267
BcoDI GTCTC 5 cut(s) 329, 447, 585, 772, 1115
BfaI CTAG 2 cut(s) 242, 1378
BfmI CTRYAG 1 cut(s) 873
BfrI CTTAAG 1 cut(s) 1187
BfuAI ACCTGC 1 cut(s) 548
BglI GCCNNNNNGGC 1 cut(s) 1369
BisI GCNGC 6 cut(s) 119, 294, 743, 876, 1104, 1370
BlsI GCNGC 6 cut(s) 120, 295, 744, 877, 1105, 1371
BmcAI AGTACT 1 cut(s) 871
Bme1390I CCNGG 2 cut(s) 114, 267
Bme18I GGWCC 4 cut(s) 263, 308, 1212, 1303
BmeRI GACNNNNNGTC 1 cut(s) 1301
BmeT110I CYCGRG 1 cut(s) 1320
BmgT120I GGNCC 5 cut(s) 263, 308, 799, 1212, 1303
BmiI GGNNCC 4 cut(s) 1152, 1214, 1250, 1305
BmrFI CCNGG 2 cut(s) 114, 267
BmsI GCATC 6 cut(s) 35, 226, 463, 784, 1132, 1171
BoxI GACNNNNGTC 1 cut(s) 1274
BpiI GAAGAC 1 cut(s) 423
BpmI CTGGAG 5 cut(s) 381, 471, 564, 1026, 1267
Bpu10I CCTNAGC 1 cut(s) 573
Bpu14I TTCGAA 1 cut(s) 693
BpuEI CTTGAG 2 cut(s) 913, 1097
BsaI GGTCTC 3 cut(s) 329, 447, 1115
BsaJI CCNNGG 5 cut(s) 113, 908, 1019, 1067, 1217
BsaWI WCCGGW 2 cut(s) 226, 1286
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse1I ACTGG 6 cut(s) 200, 704, 789, 1009, 1058, 1234
Bse3DI GCAATG 2 cut(s) 537, 1098
BseBI CCWGG 2 cut(s) 114, 267
BseDI CCNNGG 5 cut(s) 113, 908, 1019, 1067, 1217
BseGI GGATG 4 cut(s) 769, 775, 918, 967
BseLI CCNNNNNNNGG 1 cut(s) 132
BseMI GCAATG 2 cut(s) 537, 1098
BseMII CTCAG 4 cut(s) 527, 587, 785, 930
BseNI ACTGG 6 cut(s) 200, 704, 789, 1009, 1058, 1234
BseXI GCAGC 5 cut(s) 130, 305, 729, 887, 1090
BseYI CCCAGC 2 cut(s) 176, 211
BshFI GGCC 1 cut(s) 800
BsiHKAI GWGCWC 1 cut(s) 1098
BsiHKCI CYCGRG 1 cut(s) 1320
BsiSI CCGG 2 cut(s) 227, 1287
BslFI GGGAC 3 cut(s) 193, 1198, 1289
BslI CCNNNNNNNGG 1 cut(s) 132
BsmAI GTCTC 5 cut(s) 329, 447, 585, 772, 1115
BsmFI GGGAC 3 cut(s) 193, 1198, 1289
BsnI GGCC 1 cut(s) 800
Bso31I GGTCTC 3 cut(s) 329, 447, 1115
BsoBI CYCGRG 1 cut(s) 1320
Bsp119I TTCGAA 1 cut(s) 693
Bsp1286I GDGCHC 1 cut(s) 1098
Bsp143I GATC 3 cut(s) 82, 928, 1343
Bsp19I CCATGG 2 cut(s) 908, 1217
BspACI CCGC 3 cut(s) 239, 1106, 1369
BspANI GGCC 1 cut(s) 800
BspCNI CTCAG 4 cut(s) 526, 586, 784, 931
BspLI GGNNCC 4 cut(s) 1152, 1214, 1250, 1305
BspMAI CTGCAG 1 cut(s) 877
BspMI ACCTGC 1 cut(s) 548
BspPI GGATC 1 cut(s) 923
BspT104I TTCGAA 1 cut(s) 693
BspTI CTTAAG 1 cut(s) 1187
BspTNI GGTCTC 3 cut(s) 329, 447, 1115
BsrDI GCAATG 2 cut(s) 537, 1098
BsrI ACTGG 6 cut(s) 200, 704, 789, 1009, 1058, 1234
BssECI CCNNGG 5 cut(s) 113, 908, 1019, 1067, 1217
BssMI GATC 3 cut(s) 82, 928, 1343
BssNAI GTATAC 3 cut(s) 444, 480, 1126
BssT1I CCWWGG 2 cut(s) 908, 1217
Bst1107I GTATAC 3 cut(s) 444, 480, 1126
Bst2UI CCWGG 2 cut(s) 114, 267
Bst4CI ACNGT 3 cut(s) 108, 1071, 1130
Bst6I CTCTTC 1 cut(s) 294
BstAFI CTTAAG 1 cut(s) 1187
BstAPI GCANNNNNTGC 1 cut(s) 992
BstBI TTCGAA 1 cut(s) 693
BstC8I GCNNGC 4 cut(s) 123, 174, 790, 1374
BstDEI CTNAG 4 cut(s) 513, 573, 771, 939
BstDSI CCRYGG 4 cut(s) 908, 1019, 1067, 1217
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 4 cut(s) 769, 775, 918, 967
BstHHI GCGC 2 cut(s) 217, 888
BstKTI GATC 3 cut(s) 85, 931, 1346
BstMAI GTCTC 5 cut(s) 329, 447, 585, 772, 1115
BstMBI GATC 3 cut(s) 82, 928, 1343
BstMWI GCNNNNNNNGC 7 cut(s) 65, 118, 182, 290, 473, 992, 1369
BstNI CCWGG 2 cut(s) 114, 267
BstNSI RCATGY 1 cut(s) 1032
BstPAI GACNNNNGTC 1 cut(s) 1274
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 2 cut(s) 112, 265
BstSFI CTRYAG 1 cut(s) 873
BstV1I GCAGC 5 cut(s) 130, 305, 729, 887, 1090
BstV2I GAAGAC 1 cut(s) 423
BstXI CCANNNNNNTGG 1 cut(s) 360
BstZ17I GTATAC 3 cut(s) 444, 480, 1126
BsuRI GGCC 1 cut(s) 800
BtgI CCRYGG 4 cut(s) 908, 1019, 1067, 1217
BtsCI GGATG 4 cut(s) 769, 775, 918, 967
BtsI GCAGTG 3 cut(s) 159, 1039, 1152
BtsIMutI CAGTG 6 cut(s) 159, 782, 936, 1039, 1135, 1152
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 4 cut(s) 123, 174, 790, 1374
CfoI GCGC 2 cut(s) 217, 888
Cfr13I GGNCC 5 cut(s) 263, 308, 799, 1212, 1303
Csp6I GTAC 4 cut(s) 517, 687, 870, 1015
CspCI CAANNNNNGTGG 2 cut(s) 1222, 1257
CviQI GTAC 4 cut(s) 517, 687, 870, 1015
DdeI CTNAG 4 cut(s) 513, 573, 771, 939
DpnI GATC 3 cut(s) 84, 930, 1345
DpnII GATC 3 cut(s) 82, 928, 1343
DrdI GACNNNNNNGTC 1 cut(s) 764
DriI GACNNNNNGTC 1 cut(s) 1301
DseDI GACNNNNNNGTC 1 cut(s) 764
Eam1104I CTCTTC 1 cut(s) 294
Eam1105I GACNNNNNGTC 1 cut(s) 1301
EarI CTCTTC 1 cut(s) 294
Ecl136II GAGCTC 1 cut(s) 1096
Eco130I CCWWGG 2 cut(s) 908, 1217
Eco24I GRGCYC 1 cut(s) 1098
Eco31I GGTCTC 3 cut(s) 329, 447, 1115
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 4 cut(s) 263, 308, 1212, 1303
Eco53kI GAGCTC 1 cut(s) 1096
Eco57I CTGAAG 3 cut(s) 318, 417, 489
Eco88I CYCGRG 1 cut(s) 1320
Eco91I GGTNACC 1 cut(s) 5
EcoICRI GAGCTC 1 cut(s) 1096
EcoO109I RGGNCCY 1 cut(s) 1303
EcoO65I GGTNACC 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 609
EcoRII CCWGG 2 cut(s) 112, 265
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 2 cut(s) 908, 1217
EcoT38I GRGCYC 1 cut(s) 1098
ErhI CCWWGG 2 cut(s) 908, 1217
FalI AAGNNNNNCTT 4 cut(s) 743, 775, 875, 907
FaqI GGGAC 3 cut(s) 193, 1198, 1289
FauI CCCGC 1 cut(s) 1099
FauNDI CATATG 3 cut(s) 286, 355, 925
FblI GTMKAC 3 cut(s) 443, 479, 1125
Fnu4HI GCNGC 6 cut(s) 119, 294, 743, 876, 1104, 1370
FokI GGATG 4 cut(s) 762, 776, 925, 954
FriOI GRGCYC 1 cut(s) 1098
Fsp4HI GCNGC 6 cut(s) 119, 294, 743, 876, 1104, 1370
FspBI CTAG 2 cut(s) 242, 1378
GlaI GCGC 2 cut(s) 216, 887
GluI GCNGC 6 cut(s) 119, 294, 743, 876, 1104, 1370
GsaI CCCAGC 2 cut(s) 180, 215
GsuI CTGGAG 5 cut(s) 381, 471, 564, 1026, 1267
HaeIII GGCC 1 cut(s) 800
HapII CCGG 2 cut(s) 227, 1287
HhaI GCGC 2 cut(s) 217, 888
Hin6I GCGC 2 cut(s) 215, 886
HinP1I GCGC 2 cut(s) 215, 886
HindIII AAGCTT 1 cut(s) 1326
HinfI GANTC 9 cut(s) 206, 230, 349, 392, 616, 725, 747, 815, 1061
HpaII CCGG 2 cut(s) 227, 1287
HphI GGTGA 2 cut(s) 1195, 1270
Hpy166II GTNNAC 5 cut(s) 224, 444, 480, 705, 1126
Hpy188I TCNGA 7 cut(s) 436, 615, 724, 820, 952, 1060, 1343
Hpy8I GTNNAC 5 cut(s) 224, 444, 480, 705, 1126
HpyCH4III ACNGT 3 cut(s) 108, 1071, 1130
HpyCH4IV ACGT 1 cut(s) 1168
HpyF10VI GCNNNNNNNGC 7 cut(s) 65, 118, 182, 290, 473, 992, 1369
HpyF3I CTNAG 4 cut(s) 513, 573, 771, 939
HpySE526I ACGT 1 cut(s) 1168
HspAI GCGC 2 cut(s) 215, 886
Kzo9I GATC 3 cut(s) 82, 928, 1343
LmnI GCTCC 4 cut(s) 56, 290, 1248, 1289
Lsp1109I GCAGC 5 cut(s) 130, 305, 729, 887, 1090
LweI GCATC 6 cut(s) 35, 226, 463, 784, 1132, 1171
MaeI CTAG 2 cut(s) 242, 1378
MaeII ACGT 1 cut(s) 1168
MaeIII GTNAC 9 cut(s) 5, 157, 658, 755, 1000, 1156, 1169, 1191, 1276
MalI GATC 3 cut(s) 84, 930, 1345
MboI GATC 3 cut(s) 82, 928, 1343
MboII GAAGA 5 cut(s) 63, 267, 311, 423, 482
MfeI CAATTG 4 cut(s) 86, 493, 584, 1207
MhlI GDGCHC 1 cut(s) 1098
MlyI GAGTC 3 cut(s) 215, 239, 756
MmeI TCCRAC 1 cut(s) 1024
MnlI CCTC 8 cut(s) 84, 522, 582, 812, 888, 936, 1294, 1329
MroXI GAANNNNTTC 1 cut(s) 507
MseI TTAA 4 cut(s) 633, 716, 963, 1188
MslI CAYNNNNRTG 2 cut(s) 425, 996
MspA1I CMGCKG 5 cut(s) 296, 401, 449, 467, 577
MspCI CTTAAG 1 cut(s) 1187
MspI CCGG 2 cut(s) 227, 1287
MspR9I CCNGG 2 cut(s) 114, 267
MunI CAATTG 4 cut(s) 86, 493, 584, 1207
MvaI CCWGG 2 cut(s) 114, 267
MwoI GCNNNNNNNGC 7 cut(s) 65, 118, 182, 290, 473, 992, 1369
NcoI CCATGG 2 cut(s) 908, 1217
NdeI CATATG 3 cut(s) 286, 355, 925
NdeII GATC 3 cut(s) 82, 928, 1343
NlaIV GGNNCC 4 cut(s) 1152, 1214, 1250, 1305
NmuCI GTSAC 4 cut(s) 157, 755, 1156, 1276
NspI RCATGY 1 cut(s) 1032
NspV TTCGAA 1 cut(s) 693
PaeR7I CTCGAG 1 cut(s) 1320
PcsI WCGNNNNNNNCGW 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 507
PfeI GAWTC 6 cut(s) 349, 392, 616, 725, 815, 1061
PkrI GCNGC 6 cut(s) 120, 295, 744, 877, 1105, 1371
PleI GAGTC 3 cut(s) 214, 238, 755
PpsI GAGTC 3 cut(s) 214, 238, 755
PpuMI RGGWCCY 1 cut(s) 1303
PshAI GACNNNNGTC 1 cut(s) 1274
Psp124BI GAGCTC 1 cut(s) 1098
Psp5II RGGWCCY 1 cut(s) 1303
Psp6I CCWGG 2 cut(s) 112, 265
PspEI GGTNACC 1 cut(s) 5
PspFI CCCAGC 2 cut(s) 176, 211
PspGI CCWGG 2 cut(s) 112, 265
PspN4I GGNNCC 4 cut(s) 1152, 1214, 1250, 1305
PspPI GGNCC 5 cut(s) 263, 308, 799, 1212, 1303
PspPPI RGGWCCY 1 cut(s) 1303
PstI CTGCAG 1 cut(s) 877
PvuII CAGCTG 5 cut(s) 296, 401, 449, 467, 577
RsaI GTAC 4 cut(s) 518, 688, 871, 1016
RsaNI GTAC 4 cut(s) 517, 687, 870, 1015
RseI CAYNNNNRTG 2 cut(s) 425, 996
SacI GAGCTC 1 cut(s) 1098
SaqAI TTAA 4 cut(s) 633, 716, 963, 1188
SatI GCNGC 6 cut(s) 119, 294, 743, 876, 1104, 1370
Sau3AI GATC 3 cut(s) 82, 928, 1343
Sau96I GGNCC 5 cut(s) 263, 308, 799, 1212, 1303
ScaI AGTACT 1 cut(s) 871
SchI GAGTC 3 cut(s) 215, 239, 756
ScrFI CCNGG 2 cut(s) 114, 267
SduI GDGCHC 1 cut(s) 1098
SfaNI GCATC 6 cut(s) 35, 226, 463, 784, 1132, 1171
SfcI CTRYAG 1 cut(s) 873
Sfr274I CTCGAG 1 cut(s) 1320
SfuI TTCGAA 1 cut(s) 693
SinI GGWCC 4 cut(s) 263, 308, 1212, 1303
SlaI CTCGAG 1 cut(s) 1320
SmiMI CAYNNNNRTG 2 cut(s) 425, 996
SmlI CTYRAG 4 cut(s) 892, 1112, 1187, 1320
SmoI CTYRAG 4 cut(s) 892, 1112, 1187, 1320
SsiI CCGC 3 cut(s) 239, 1106, 1369
SspMI CTAG 2 cut(s) 242, 1378
SstI GAGCTC 1 cut(s) 1098
StyD4I CCNGG 2 cut(s) 112, 265
StyI CCWWGG 2 cut(s) 908, 1217
TaaI ACNGT 3 cut(s) 108, 1071, 1130
TaiI ACGT 1 cut(s) 1171
TaqI TCGA 3 cut(s) 693, 1315, 1321
TatI WGTACW 2 cut(s) 516, 869
TauI GCSGC 1 cut(s) 1372
TfiI GAWTC 6 cut(s) 349, 392, 616, 725, 815, 1061
Tru1I TTAA 4 cut(s) 633, 716, 963, 1188
Tru9I TTAA 4 cut(s) 633, 716, 963, 1188
TscAI CASTG 6 cut(s) 166, 789, 943, 1039, 1135, 1152
TseFI GTSAC 4 cut(s) 157, 755, 1156, 1276
TseI GCWGC 5 cut(s) 118, 293, 742, 875, 1103
Tsp45I GTSAC 4 cut(s) 157, 755, 1156, 1276
TspDTI ATGAA 5 cut(s) 39, 305, 362, 405, 1321
TspRI CASTG 6 cut(s) 166, 789, 943, 1039, 1135, 1152
Vha464I CTTAAG 1 cut(s) 1187
VpaK11BI GGWCC 4 cut(s) 263, 308, 1212, 1303
XapI RAATTY 2 cut(s) 376, 609
XceI RCATGY 1 cut(s) 1032
XcmI CCANNNNNNNNNTGG 1 cut(s) 680
XhoI CTCGAG 1 cut(s) 1320
XmiI GTMKAC 3 cut(s) 443, 479, 1125
XmnI GAANNNNTTC 1 cut(s) 507
XspI CTAG 2 cut(s) 242, 1378
ZrmI AGTACT 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.