AT2G36640

embryonic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012043
Physical Location & Seq
Reverse (-)
15356830 .. 15358619
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G36640.1

Sequence Viewer

Length: 1347 bp
ATGGCGTCAGACAAACAAAAGGCGGAGAGAGCCGAGGTTGCGGCGAGGCTAGCGGCTGAGGACTTGCATGACATTAACAAATCCGGTGGTGCTGATGTCACAATGTATAAGGTGACGGAGAGAACAACTGAACATCCACCGGAGCAAGATAGGCCCGGTGTGATAGGATCAGTGTTCAGGGCTGTCCAAGGAACGTATGAGCATGCGAGAGACGCTGTAGTTGGAAAAACCCACGAAGCGGCTGAGTCTACCAAAGAAGGAGCTCAGATAGCTTCAGAGAAAGCGGTTGGAGCAAAGGACGCAACCGTCGAGAAAGCTAAGGAAACCGCTGATTATACTGCGGAGAAGGTGGGTGAGTATAAAGACTATACGGTTGATAAAGCTAAAGAGGCTAAGGACACAACTGCAGAGAAGGCGAAGGAGACTGCTAATTATACTGCGGATAAGGCGGTGGAAGCAAAGGATAAGACGGCGGAGAAGATTGGTGAGTACAAAGACTATGCGGTGGATAAGGCAGTAGAAGCTAAAGATAAGACAGCGGAGAAGGCGAAGGAGACTGCGAATTATACGGCGGATAAGGCTAAAGAGGCTAAGGACAAGACGGCTGAGAAGGTTGGTGAGTATAAGGATTACACGGTGGACAAGGCCGTGGAAGCTAGGGATTACACAGCGGAGAAGGCTATTGAAGCAAAGGATAAGACAGCTGAGAAGACTGGAGAGTATAAGGACTATACGGTGGAGAAGGCGACGGAGGGGAAAGATGTTACGGTGAGTAAGCTAGGAGAGCTGAAGGATAGTGCCGTTGAGACAGCGAAGAGAGCTATGGGTTTCTTGTCGGGGAAGACAGAGGAGGCCAAAGGAAAAGCTGTGGAGACCAAAGATACTGCCAAGGAAAACATGGAGAAAGCTGGAGAAGTAACAAGACAAAAGATGGAGGAAATGAGATTGGAAGGTAAAGAGCTCAAAGAAGAAGCTGGAGCAAAAGCCCAAGAGGCATCTCAAAAGACTAGGGAGAGTACTGAGTCGGGAGCTCAAAAAGCCGAAGAGACCAAAGATTCTGCTGCCGTGAGGGGAAATGAAGCGAAAGGGACTATTTTTGGTGCATTAGGGAATGTAACGGAAGCAATAAAGAGCAAACTGACAATGCCATCAGACATTGTGGAGGAAACACGCGCGGCACGTGAGCATGGAGGGACGGGTAGGACTGTGGTTGAAGTCAAGGTCGAGGATTCAAAGCCGGGTAAGGTGGCGACTTCACTGAAGGCGTCGGATCAAATGACCGGTCAAACATTCAACGACGTTGGACGGATGGATGATGATGCTCGGAAAGATAAGGGAAAGCTGTGA

Protein Analysis

448

Amino Acids

48.49

Weight (kDa)

5.43

Isoelectric Point (pI)

12.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_4 PF02987 90 - 116 7.5e-06 Late embryogenesis abundant protein
LEA_4 PF02987 99 - 142 4.6e-14 Late embryogenesis abundant protein
LEA_4 PF02987 144 - 186 8.5e-16 Late embryogenesis abundant protein
LEA_4 PF02987 188 - 223 1.7e-13 Late embryogenesis abundant protein
LEA_4 PF02987 221 - 263 1.6e-19 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012043)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36640 AT2G36640 AT3G53040
fragaria_vesca FvH4_6g17400
malus_domestica MD09G1267600.v1.1
prunus_persica Prupe.7G121500_v2.0.a1 Prupe.7G121500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0471651
rosa_laevigata RLG00000024148
rosa_multiflora Rmu_co8483845.1_g000001 Rmu_sc0005631.1_g000008
rosa_roxburghii Rroxscaffold_6G00409690
rosa_rugosa Rorug03G0118600
rosa_samantha Rh3AG169800 Rh3BG195500 Rh3CG186200 Rh3CG195500 Rh3DG179500
rosa_wichuraiana Rw0G008960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 305
AccI GTMKAC 1 cut(s) 248
AccII CGCG 2 cut(s) 1173, 1175
AclWI GGATC 2 cut(s) 175, 1278
AcuI CTGAAG 3 cut(s) 258, 809, 1280
AcvI CACGTG 1 cut(s) 1181
AcyI GRCGYC 2 cut(s) 5, 1265
AfaI GTAC 2 cut(s) 491, 1018
AfiI CCNNNNNNNGG 1 cut(s) 238
AgeI ACCGGT 1 cut(s) 1280
AgsI TTSAA 4 cut(s) 686, 1214, 1233, 1294
Alw21I GWGCWC 3 cut(s) 265, 963, 1033
Alw26I GTCTC 6 cut(s) 204, 416, 548, 800, 866, 1040
AlwI GGATC 2 cut(s) 175, 1278
AoxI GGCC 3 cut(s) 152, 645, 852
ApeKI GCWGC 1 cut(s) 1061
ArsI GACNNNNNNTTYG 2 cut(s) 1267, 1299
AsiGI ACCGGT 1 cut(s) 1280
AspLEI GCGC 1 cut(s) 1175
AspS9I GGNCC 1 cut(s) 153
AsuC2I CCSGG 2 cut(s) 156, 1239
AsuHPI GGTGA 5 cut(s) 124, 365, 497, 629, 781
AsuNHI GCTAGC 1 cut(s) 49
BanII GRGCYC 3 cut(s) 265, 963, 1033
BbrPI CACGTG 1 cut(s) 1181
BbsI GAAGAC 2 cut(s) 716, 848
Bbv12I GWGCWC 3 cut(s) 265, 963, 1033
BbvCI CCTCAGC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 1048
BccI CCATC 3 cut(s) 925, 1156, 1303
BceAI ACGGC 6 cut(s) 486, 585, 618, 632, 785, 1049
BcnI CCSGG 2 cut(s) 156, 1239
BcoDI GTCTC 6 cut(s) 204, 416, 548, 800, 866, 1040
BfaI CTAG 4 cut(s) 50, 657, 779, 1008
BfmI CTRYAG 2 cut(s) 216, 405
BglI GCCNNNNNGGC 1 cut(s) 992
BisI GCNGC 5 cut(s) 42, 54, 240, 1062, 1176
BlsI GCNGC 5 cut(s) 43, 55, 241, 1063, 1177
BmcAI AGTACT 1 cut(s) 1018
Bme1390I CCNGG 2 cut(s) 156, 1239
BmgT120I GGNCC 1 cut(s) 153
BmrFI CCNGG 2 cut(s) 156, 1239
BmsI GCATC 2 cut(s) 1004, 1309
BmtI GCTAGC 1 cut(s) 53
BpiI GAAGAC 2 cut(s) 716, 848
BpmI CTGGAG 3 cut(s) 735, 930, 996
Bpu10I CCTNAGC 4 cut(s) 57, 318, 393, 591
BpuMI CCSGG 2 cut(s) 156, 1239
BsaAI YACGTR 1 cut(s) 1181
BsaHI GRCGYC 2 cut(s) 5, 1265
BsaI GGTCTC 2 cut(s) 866, 1040
BsaJI CCNNGG 4 cut(s) 33, 187, 648, 888
BsaWI WCCGGW 3 cut(s) 83, 139, 1280
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse118I RCCGGY 1 cut(s) 1280
Bse1I ACTGG 1 cut(s) 718
BseDI CCNNGG 4 cut(s) 33, 187, 648, 888
BseGI GGATG 3 cut(s) 133, 1314, 1318
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMII CTCAG 6 cut(s) 48, 234, 278, 597, 696, 1011
BseNI ACTGG 1 cut(s) 718
BseRI GAGGAG 1 cut(s) 863
BseXI GCAGC 1 cut(s) 1048
Bsh1236I CGCG 2 cut(s) 1173, 1175
BshFI GGCC 3 cut(s) 154, 647, 854
BshTI ACCGGT 1 cut(s) 1280
BsiHKAI GWGCWC 3 cut(s) 265, 963, 1033
BsiSI CCGG 5 cut(s) 84, 140, 156, 1238, 1281
BslFI GGGAC 2 cut(s) 1102, 1207
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 6 cut(s) 204, 416, 548, 800, 866, 1040
BsmBI CGTCTC 1 cut(s) 204
BsmFI GGGAC 2 cut(s) 1102, 1207
BsnI GGCC 3 cut(s) 154, 647, 854
Bso31I GGTCTC 2 cut(s) 866, 1040
Bsp1286I GDGCHC 3 cut(s) 265, 963, 1033
Bsp143I GATC 2 cut(s) 167, 1270
BspANI GGCC 3 cut(s) 154, 647, 854
BspCNI CTCAG 6 cut(s) 49, 235, 277, 598, 697, 1012
BspFNI CGCG 2 cut(s) 1173, 1175
BspMAI CTGCAG 1 cut(s) 409
BspOI GCTAGC 1 cut(s) 53
BspPI GGATC 2 cut(s) 175, 1278
BspTNI GGTCTC 2 cut(s) 866, 1040
BsrFI RCCGGY 1 cut(s) 1280
BsrI ACTGG 1 cut(s) 718
BssAI RCCGGY 1 cut(s) 1280
BssECI CCNNGG 4 cut(s) 33, 187, 648, 888
BssMI GATC 2 cut(s) 167, 1270
BssNI GRCGYC 2 cut(s) 5, 1265
BssT1I CCWWGG 2 cut(s) 187, 888
Bst4CI ACNGT 6 cut(s) 307, 373, 637, 736, 769, 1207
Bst6I CTCTTC 2 cut(s) 809, 1038
BstACI GRCGYC 2 cut(s) 5, 1265
BstBAI YACGTR 1 cut(s) 1181
BstC8I GCNNGC 2 cut(s) 51, 204
BstDEI CTNAG 9 cut(s) 57, 243, 264, 318, 393, 591, 606, 705, 1020
BstDSI CCRYGG 1 cut(s) 648
BstF5I GGATG 3 cut(s) 133, 1314, 1318
BstFNI CGCG 2 cut(s) 1173, 1175
BstHHI GCGC 1 cut(s) 1175
BstKTI GATC 2 cut(s) 170, 1273
BstMAI GTCTC 6 cut(s) 204, 416, 548, 800, 866, 1040
BstMBI GATC 2 cut(s) 167, 1270
BstNSI RCATGY 1 cut(s) 206
BstSCI CCNGG 2 cut(s) 154, 1237
BstSFI CTRYAG 2 cut(s) 216, 405
BstUI CGCG 2 cut(s) 1173, 1175
BstV1I GCAGC 1 cut(s) 1048
BstV2I GAAGAC 2 cut(s) 716, 848
BsuRI GGCC 3 cut(s) 154, 647, 854
BtgI CCRYGG 1 cut(s) 648
BtsCI GGATG 3 cut(s) 133, 1314, 1318
BtsIMutI CAGTG 2 cut(s) 177, 1256
Cac8I GCNNGC 2 cut(s) 51, 204
CfoI GCGC 1 cut(s) 1175
Cfr10I RCCGGY 1 cut(s) 1280
Cfr13I GGNCC 1 cut(s) 153
CseI GACGC 3 cut(s) 221, 308, 1254
Csp6I GTAC 2 cut(s) 490, 1017
CspAI ACCGGT 1 cut(s) 1280
CspCI CAANNNNNGTGG 2 cut(s) 67, 102
CviAII CATG 4 cut(s) 68, 203, 898, 1187
CviQI GTAC 2 cut(s) 490, 1017
DdeI CTNAG 9 cut(s) 57, 243, 264, 318, 393, 591, 606, 705, 1020
DpnI GATC 2 cut(s) 169, 1272
DpnII GATC 2 cut(s) 167, 1270
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
Eam1104I CTCTTC 2 cut(s) 809, 1038
EarI CTCTTC 2 cut(s) 809, 1038
EciI GGCGGA 3 cut(s) 38, 488, 587
Ecl136II GAGCTC 3 cut(s) 263, 961, 1031
Eco130I CCWWGG 2 cut(s) 187, 888
Eco24I GRGCYC 3 cut(s) 265, 963, 1033
Eco31I GGTCTC 2 cut(s) 866, 1040
Eco53kI GAGCTC 3 cut(s) 263, 961, 1031
Eco57I CTGAAG 3 cut(s) 258, 809, 1280
Eco72I CACGTG 1 cut(s) 1181
EcoICRI GAGCTC 3 cut(s) 263, 961, 1031
EcoT14I CCWWGG 2 cut(s) 187, 888
EcoT38I GRGCYC 3 cut(s) 265, 963, 1033
ErhI CCWWGG 2 cut(s) 187, 888
Esp3I CGTCTC 1 cut(s) 204
FaeI CATG 4 cut(s) 71, 206, 901, 1190
FaqI GGGAC 2 cut(s) 1102, 1207
FatI CATG 4 cut(s) 67, 202, 897, 1186
FblI GTMKAC 1 cut(s) 248
Fnu4HI GCNGC 5 cut(s) 42, 54, 240, 1062, 1176
FokI GGATG 3 cut(s) 120, 1321, 1325
FriOI GRGCYC 3 cut(s) 265, 963, 1033
Fsp4HI GCNGC 5 cut(s) 42, 54, 240, 1062, 1176
FspBI CTAG 4 cut(s) 50, 657, 779, 1008
GlaI GCGC 1 cut(s) 1174
GluI GCNGC 5 cut(s) 42, 54, 240, 1062, 1176
GsuI CTGGAG 3 cut(s) 735, 930, 996
HaeIII GGCC 3 cut(s) 154, 647, 854
HapII CCGG 5 cut(s) 84, 140, 156, 1238, 1281
HgaI GACGC 3 cut(s) 221, 308, 1254
HhaI GCGC 1 cut(s) 1175
Hin1I GRCGYC 2 cut(s) 5, 1265
Hin1II CATG 4 cut(s) 71, 206, 901, 1190
Hin6I GCGC 1 cut(s) 1173
HinP1I GCGC 1 cut(s) 1173
HinfI GANTC 4 cut(s) 245, 1022, 1055, 1229
HpaII CCGG 5 cut(s) 84, 140, 156, 1238, 1281
HphI GGTGA 5 cut(s) 124, 365, 497, 629, 781
Hpy166II GTNNAC 2 cut(s) 249, 640
Hpy188I TCNGA 6 cut(s) 10, 267, 277, 1153, 1270, 1326
Hpy188III TCNNGA 2 cut(s) 310, 1026
Hpy8I GTNNAC 2 cut(s) 249, 640
Hpy99I CGWCG 4 cut(s) 311, 751, 1270, 1301
HpyCH4III ACNGT 6 cut(s) 307, 373, 637, 736, 769, 1207
HpyCH4IV ACGT 3 cut(s) 194, 1180, 1299
HpyCH4V TGCA 3 cut(s) 67, 407, 1103
HpyF3I CTNAG 9 cut(s) 57, 243, 264, 318, 393, 591, 606, 705, 1020
HpySE526I ACGT 3 cut(s) 194, 1180, 1299
Hsp92I GRCGYC 2 cut(s) 5, 1265
Hsp92II CATG 4 cut(s) 71, 206, 901, 1190
HspAI GCGC 1 cut(s) 1173
Kzo9I GATC 2 cut(s) 167, 1270
LmnI GCTCC 5 cut(s) 142, 260, 290, 977, 1028
LpnPI CCDG 9 cut(s) 97, 153, 163, 169, 699, 894, 960, 1251, 1294
Lsp1109I GCAGC 1 cut(s) 1048
LweI GCATC 2 cut(s) 1004, 1309
MaeI CTAG 4 cut(s) 50, 657, 779, 1008
MaeII ACGT 3 cut(s) 194, 1180, 1299
MaeIII GTNAC 5 cut(s) 97, 112, 763, 916, 1114
MalI GATC 2 cut(s) 169, 1272
MboI GATC 2 cut(s) 167, 1270
MboII GAAGA 6 cut(s) 490, 721, 826, 853, 980, 1055
MhlI GDGCHC 3 cut(s) 265, 963, 1033
MluCI AATT 2 cut(s) 430, 562
MlyI GAGTC 2 cut(s) 254, 1031
MmeI TCCRAC 4 cut(s) 202, 268, 1248, 1282
MseI TTAA 1 cut(s) 75
MspA1I CMGCKG 4 cut(s) 329, 539, 671, 704
MspI CCGG 5 cut(s) 84, 140, 156, 1238, 1281
MspR9I CCNGG 2 cut(s) 156, 1239
MvnI CGCG 2 cut(s) 1173, 1175
NciI CCSGG 2 cut(s) 156, 1239
NdeII GATC 2 cut(s) 167, 1270
NheI GCTAGC 1 cut(s) 49
NlaIII CATG 4 cut(s) 71, 206, 901, 1190
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 2 cut(s) 97, 112
NspI RCATGY 1 cut(s) 206
PaeI GCATGC 1 cut(s) 206
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 1177
PfeI GAWTC 2 cut(s) 1055, 1229
PinAI ACCGGT 1 cut(s) 1280
PkrI GCNGC 5 cut(s) 43, 55, 241, 1063, 1177
PleI GAGTC 2 cut(s) 253, 1030
PmaCI CACGTG 1 cut(s) 1181
PmlI CACGTG 1 cut(s) 1181
PpsI GAGTC 2 cut(s) 253, 1030
Ppu21I YACGTR 1 cut(s) 1181
Psp124BI GAGCTC 3 cut(s) 265, 963, 1033
PspCI CACGTG 1 cut(s) 1181
PspPI GGNCC 1 cut(s) 153
PstI CTGCAG 1 cut(s) 409
PvuII CAGCTG 1 cut(s) 704
RsaI GTAC 2 cut(s) 491, 1018
RsaNI GTAC 2 cut(s) 490, 1017
SacI GAGCTC 3 cut(s) 265, 963, 1033
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 5 cut(s) 42, 54, 240, 1062, 1176
Sau3AI GATC 2 cut(s) 167, 1270
Sau96I GGNCC 1 cut(s) 153
ScaI AGTACT 1 cut(s) 1018
SchI GAGTC 2 cut(s) 254, 1031
ScrFI CCNGG 2 cut(s) 156, 1239
SduI GDGCHC 3 cut(s) 265, 963, 1033
SfaNI GCATC 2 cut(s) 1004, 1309
SfcI CTRYAG 2 cut(s) 216, 405
SphI GCATGC 1 cut(s) 206
Sse9I AATT 2 cut(s) 430, 562
SspMI CTAG 4 cut(s) 50, 657, 779, 1008
SstI GAGCTC 3 cut(s) 265, 963, 1033
StyD4I CCNGG 2 cut(s) 154, 1237
StyI CCWWGG 2 cut(s) 187, 888
TaaI ACNGT 6 cut(s) 307, 373, 637, 736, 769, 1207
TaiI ACGT 3 cut(s) 197, 1183, 1302
TaqI TCGA 2 cut(s) 309, 1224
TasI AATT 2 cut(s) 430, 562
TatI WGTACW 2 cut(s) 489, 1016
TauI GCSGC 4 cut(s) 44, 56, 242, 1178
TfiI GAWTC 2 cut(s) 1055, 1229
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 2 cut(s) 177, 1263
TseFI GTSAC 2 cut(s) 97, 112
TseI GCWGC 1 cut(s) 1061
Tsp45I GTSAC 2 cut(s) 97, 112
TspDTI ATGAA 1 cut(s) 1092
TspGWI ACGGA 4 cut(s) 131, 764, 1133, 1321
TspRI CASTG 2 cut(s) 177, 1263
XceI RCATGY 1 cut(s) 206
XcmI CCANNNNNNNNNTGG 1 cut(s) 895
XmiI GTMKAC 1 cut(s) 248
XspI CTAG 4 cut(s) 50, 657, 779, 1008
ZrmI AGTACT 1 cut(s) 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.