FvH4_6g17400

Embryonic protein DC-8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012043
Physical Location & Seq
Forward (+)
11335913 .. 11337943
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17400.t1

Sequence Viewer

Length: 1563 bp
ATGGCGTCGTCGAAGCAGTTCAAGGAGGAGAGAGCCGAGGCAGAGGCGAGGAAAGCCGCGCTTGATCTGAAAGATGTCAACAGAGAAAGAGAGAACCAAACAGCTGGGGTGAAGATGGACAGGGACATGTACGGCCAGCCAAGAGAGTCGTCTCATGTGCAACAAGAAGCGAAGCCTGGTGTTATTGGTTCTGTTTTTAGAGCTGTAACCGGCACACTGGAGCATGCAAAGGACGCTGTTGTGGGCAAAAGCCACGACGCTTCTGATGCAGCAAGAGAGCGTTCTGAATACACCACACTGAAGGCAAAGGAGACAACTGATGCTCCTGGTGAGAAACTGGATGAGTATAAGGAGGCCGTGGCTCAGCCTGCGAAGGAGGCTGCAGATAGAGCGGCCAAGAAGACGATGGCGATGAAGGACACTACTGCACAGAAGGCCAAGGAGACAGCGGACAAGGCGAGAGAGACCAAGGAGTCTGCTAAGGGAAATGTGAATGAGTACGCGGGTTATACTGCGCAGAAGGCCACAGAGGCTAAGGATGCTGCGGCGGAGAAGGCCAAGCAGACGAAGGACTATGTTGCCGATACCGCAAATGAAGCAGCAGAGAAGGCGAAAGAGACTAAAGATGCAGCTATGAGGAAGGCTGGGGAGTATACTGATTACACTGCTCAGAAGGCCCAGGAGGCCAAGGATACCACAGTAGGGAAGGCTAAAGAGTACACCAATTATGCTGCTGACAAGGCCAAGGAGGGCAAGGACACTACCGTGGGGAAGGCCAGTGAATACACGAACTATGCTGCGGAGAAGGCAAAGGAGGCCAAAGACACTACAGTAGAGAAAGCGAAGGAAGCCAAGGATACTACGGCAGAGAAGACGAAAGAGACCGCAGAGGCAGCCAAAAATAAGACTAACGATGCTGCGGAGTATACGGCTGAGAAAGCAAAAGAAGGGAAGGACACTACTATGAGCAAGTTGGGTGGGCTCATGAATTACTTCACCGGTAAGAAAGAAGAGACCAAAGAGAAGGCTGCAGAGACGGCCGAGACTGTCAAAGAGAACACTAAACAGATGGTCGACACAGCGAAGGAGAAGAATAAAGAGACTGCGGAGGCAACCAAACATAAGACCAGAGAGGCAGCCGAGAAAACCAAGGAAACGTTGAGCGAGACCGAGGAGGATGCTAGGAGAAAAATGGAGGAGTTGAGGCTAATGGAGAAAGGTTATGAAGCGCGAGCAGGAGACATGGACCGTGAGACTGAGGCAGATAAGGGCACTGCCGCCAGGAGCAACATTTTCAGTACACTTGGGAGTGTGACTGATGCTATCAAGAGCAAGCTGACTCATCCAAGCGATGTGGTAGGGGAGTCGCGTGCCACACATCAGGATGTACCTGTGGAGAAGGTGGAAATCGACATCAAGGACACTCGCCCAGGGGCTGTTGCGGCGGCGCTTAGGAAGTCCGACCATCCTGGTCAAATGGGAGATGAGGGCACAGTGGTTGTGGAGCGGCGCGTGGTGGATGAGGCCGGAAATGTGCGCGTGGACCGCAGCGGCAAGATGTGA

Protein Analysis

521

Amino Acids

56.75

Weight (kDa)

6.06

Isoelectric Point (pI)

21.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_4 PF02987 149 - 188 1.8e-07 Late embryogenesis abundant protein
LEA_4 PF02987 170 - 200 6.6e-06 Late embryogenesis abundant protein
LEA_4 PF02987 199 - 240 1.8e-14 Late embryogenesis abundant protein
LEA_4 PF02987 241 - 284 3e-16 Late embryogenesis abundant protein
LEA_4 PF02987 285 - 326 8.7e-08 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012043)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36640 AT2G36640 AT3G53040
fragaria_vesca FvH4_6g17400
malus_domestica MD09G1267600.v1.1
prunus_persica Prupe.7G121500_v2.0.a1 Prupe.7G121500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0471651
rosa_laevigata RLG00000024148
rosa_multiflora Rmu_co8483845.1_g000001 Rmu_sc0005631.1_g000008
rosa_roxburghii Rroxscaffold_6G00409690
rosa_rugosa Rorug03G0118600
rosa_samantha Rh3AG169800 Rh3BG195500 Rh3CG186200 Rh3CG195500 Rh3DG179500
rosa_wichuraiana Rw0G008960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 472
Acc16I TGCGCA 1 cut(s) 516
AccBSI CCGCTC 2 cut(s) 392, 1507
AccI GTMKAC 3 cut(s) 653, 926, 1074
AccII CGCG 6 cut(s) 59, 503, 1231, 1369, 1512, 1539
AclI AACGTT 1 cut(s) 1157
AcoI YGGCCR 3 cut(s) 133, 393, 1038
AcuI CTGAAG 1 cut(s) 320
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 131, 500, 719, 1300, 1389
AfiI CCNNNNNNNGG 1 cut(s) 702
AflIII ACRYGT 1 cut(s) 126
AgeI ACCGGT 1 cut(s) 998
AgsI TTSAA 1 cut(s) 22
AjnI CCWGG 6 cut(s) 175, 325, 678, 1280, 1429, 1468
AleI CACNNNNGTG 1 cut(s) 764
AluBI AGCT 4 cut(s) 104, 203, 632, 1336
AluI AGCT 4 cut(s) 104, 203, 632, 1336
AlwNI CAGNNNCTG 1 cut(s) 1436
AsiGI ACCGGT 1 cut(s) 998
Asp700I GAANNNNTTC 2 cut(s) 17, 992
AspLEI GCGC 6 cut(s) 61, 517, 1231, 1450, 1512, 1539
AspS9I GGNCC 3 cut(s) 676, 1246, 1543
AsuHPI GGTGA 3 cut(s) 121, 341, 988
AvaII GGWCC 2 cut(s) 1246, 1543
BaeGI GKGCMC 2 cut(s) 1274, 1493
BaeI ACNNNNGTAYC 2 cut(s) 684, 717
BanII GRGCYC 1 cut(s) 984
BbsI GAAGAC 2 cut(s) 407, 878
BccI CCATC 4 cut(s) 109, 400, 1063, 1473
BceAI ACGGC 5 cut(s) 148, 341, 879, 945, 1053
BcgI CGANNNNNNTGC 2 cut(s) 1160, 1194
BciT130I CCWGG 6 cut(s) 177, 327, 680, 1282, 1431, 1470
BciVI GTATCC 2 cut(s) 685, 850
BfaI CTAG 1 cut(s) 1182
BfmI CTRYAG 3 cut(s) 381, 828, 1029
BfoI RGCGCY 1 cut(s) 1451
BfuI GTATCC 2 cut(s) 685, 850
BglI GCCNNNNNGGC 2 cut(s) 530, 683
BlpI GCTNAGC 1 cut(s) 363
Bme1390I CCNGG 6 cut(s) 177, 327, 680, 1282, 1431, 1470
Bme18I GGWCC 2 cut(s) 1246, 1543
BmgT120I GGNCC 3 cut(s) 676, 1246, 1543
BmrFI CCNGG 6 cut(s) 177, 327, 680, 1282, 1431, 1470
BmsI GCATC 7 cut(s) 256, 310, 529, 616, 904, 1168, 1309
BpiI GAAGAC 2 cut(s) 407, 878
BpmI CTGGAG 1 cut(s) 239
Bpu10I CCTNAGC 3 cut(s) 480, 534, 1451
Bpu1102I GCTNAGC 1 cut(s) 363
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 4 cut(s) 458, 875, 1007, 1160
BsaWI WCCGGW 1 cut(s) 998
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bsc4I CCNNNNNNNGG 1 cut(s) 702
Bse118I RCCGGY 2 cut(s) 209, 998
Bse1I ACTGG 3 cut(s) 222, 342, 777
BseBI CCWGG 6 cut(s) 177, 327, 680, 1282, 1431, 1470
BseGI GGATG 7 cut(s) 346, 544, 1183, 1342, 1390, 1465, 1525
BseLI CCNNNNNNNGG 1 cut(s) 702
BseMII CTCAG 4 cut(s) 377, 683, 924, 1248
BseNI ACTGG 3 cut(s) 222, 342, 777
BseRI GAGGAG 3 cut(s) 41, 1187, 1211
BseSI GKGCMC 2 cut(s) 1274, 1493
BseX3I CGGCCG 1 cut(s) 1038
BseYI CCCAGC 2 cut(s) 104, 644
BsgI GTGCAG 1 cut(s) 411
Bsh1236I CGCG 6 cut(s) 59, 503, 1231, 1369, 1512, 1539
Bsh1285I CGRYCG 1 cut(s) 1041
BshTI ACCGGT 1 cut(s) 998
BsiEI CGRYCG 1 cut(s) 1041
BsiSI CCGG 3 cut(s) 210, 999, 1527
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 1 cut(s) 702
BsmBI CGTCTC 2 cut(s) 156, 1028
BsmFI GGGAC 1 cut(s) 137
Bso31I GGTCTC 4 cut(s) 458, 875, 1007, 1160
Bsp1286I GDGCHC 3 cut(s) 984, 1274, 1493
Bsp143I GATC 1 cut(s) 64
Bsp1720I GCTNAGC 1 cut(s) 363
BspCNI CTCAG 4 cut(s) 376, 682, 925, 1249
BspFNI CGCG 6 cut(s) 59, 503, 1231, 1369, 1512, 1539
BspHI TCATGA 1 cut(s) 984
BspMAI CTGCAG 2 cut(s) 385, 1033
BspTNI GGTCTC 4 cut(s) 458, 875, 1007, 1160
BsrBI CCGCTC 2 cut(s) 392, 1507
BsrFI RCCGGY 2 cut(s) 209, 998
BsrI ACTGG 3 cut(s) 222, 342, 777
BssAI RCCGGY 2 cut(s) 209, 998
BssMI GATC 1 cut(s) 64
BssNAI GTATAC 2 cut(s) 654, 927
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 6 cut(s) 438, 468, 687, 744, 852, 1149
Bst1107I GTATAC 2 cut(s) 654, 927
Bst2UI CCWGG 6 cut(s) 177, 327, 680, 1282, 1431, 1470
Bst4CI ACNGT 6 cut(s) 700, 766, 832, 1048, 1250, 1495
Bst6I CTCTTC 1 cut(s) 1005
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 6 cut(s) 137, 225, 369, 1233, 1334, 1371
BstDEI CTNAG 7 cut(s) 363, 480, 534, 669, 933, 1257, 1451
BstDSI CCRYGG 2 cut(s) 357, 765
BstF5I GGATG 7 cut(s) 346, 544, 1183, 1342, 1390, 1465, 1525
BstFNI CGCG 6 cut(s) 59, 503, 1231, 1369, 1512, 1539
BstH2I RGCGCY 1 cut(s) 1451
BstHHI GCGC 6 cut(s) 61, 517, 1231, 1450, 1512, 1539
BstKTI GATC 1 cut(s) 67
BstMBI GATC 1 cut(s) 64
BstMCI CGRYCG 1 cut(s) 1041
BstNI CCWGG 6 cut(s) 177, 327, 680, 1282, 1431, 1470
BstNSI RCATGY 2 cut(s) 130, 227
BstSCI CCNGG 6 cut(s) 175, 325, 678, 1280, 1429, 1468
BstSFI CTRYAG 3 cut(s) 381, 828, 1029
BstSLI GKGCMC 2 cut(s) 1274, 1493
BstUI CGCG 6 cut(s) 59, 503, 1231, 1369, 1512, 1539
BstV2I GAAGAC 2 cut(s) 407, 878
BstXI CCANNNNNNTGG 1 cut(s) 104
BstZ17I GTATAC 2 cut(s) 654, 927
BstZI CGGCCG 1 cut(s) 1038
BsuI GTATCC 2 cut(s) 685, 850
BtgI CCRYGG 2 cut(s) 357, 765
BtgZI GCGATG 2 cut(s) 425, 1365
BtsCI GGATG 7 cut(s) 346, 544, 1183, 1342, 1390, 1465, 1525
BtsI GCAGTG 2 cut(s) 663, 1272
BtsIMutI CAGTG 6 cut(s) 215, 296, 663, 784, 1272, 1500
Cac8I GCNNGC 6 cut(s) 137, 225, 369, 1233, 1334, 1371
CaiI CAGNNNCTG 1 cut(s) 1436
CciI TCATGA 1 cut(s) 984
CfoI GCGC 6 cut(s) 61, 517, 1231, 1450, 1512, 1539
Cfr10I RCCGGY 2 cut(s) 209, 998
Cfr13I GGNCC 3 cut(s) 676, 1246, 1543
CseI GACGC 2 cut(s) 242, 266
Csp6I GTAC 5 cut(s) 130, 499, 718, 1299, 1388
CspAI ACCGGT 1 cut(s) 998
CspCI CAANNNNNGTGG 4 cut(s) 958, 993, 1335, 1370
CviAII CATG 5 cut(s) 127, 155, 224, 985, 1243
CviQI GTAC 5 cut(s) 130, 499, 718, 1299, 1388
DdeI CTNAG 7 cut(s) 363, 480, 534, 669, 933, 1257, 1451
DpnI GATC 1 cut(s) 66
DpnII GATC 1 cut(s) 64
DrdI GACNNNNNNGTC 1 cut(s) 472
DseDI GACNNNNNNGTC 1 cut(s) 472
EaeI YGGCCR 3 cut(s) 133, 393, 1038
EagI CGGCCG 1 cut(s) 1038
Eam1104I CTCTTC 1 cut(s) 1005
EarI CTCTTC 1 cut(s) 1005
EciI GGCGGA 1 cut(s) 563
EclXI CGGCCG 1 cut(s) 1038
Eco130I CCWWGG 6 cut(s) 438, 468, 687, 744, 852, 1149
Eco24I GRGCYC 1 cut(s) 984
Eco31I GGTCTC 4 cut(s) 458, 875, 1007, 1160
Eco47I GGWCC 2 cut(s) 1246, 1543
Eco52I CGGCCG 1 cut(s) 1038
Eco57I CTGAAG 1 cut(s) 320
EcoRII CCWGG 6 cut(s) 175, 325, 678, 1280, 1429, 1468
EcoT14I CCWWGG 6 cut(s) 438, 468, 687, 744, 852, 1149
EcoT38I GRGCYC 1 cut(s) 984
ErhI CCWWGG 6 cut(s) 438, 468, 687, 744, 852, 1149
Esp3I CGTCTC 2 cut(s) 156, 1028
FaeI CATG 5 cut(s) 130, 158, 227, 988, 1246
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 1 cut(s) 137
FatI CATG 5 cut(s) 126, 154, 223, 984, 1242
FauI CCCGC 1 cut(s) 496
FblI GTMKAC 3 cut(s) 653, 926, 1074
FokI GGATG 7 cut(s) 353, 551, 1190, 1329, 1397, 1452, 1532
FriOI GRGCYC 1 cut(s) 984
FspBI CTAG 1 cut(s) 1182
FspI TGCGCA 1 cut(s) 516
GlaI GCGC 6 cut(s) 60, 516, 1230, 1449, 1511, 1538
GsaI CCCAGC 2 cut(s) 108, 648
GsuI CTGGAG 1 cut(s) 239
HaeII RGCGCY 1 cut(s) 1451
HapII CCGG 3 cut(s) 210, 999, 1527
HgaI GACGC 2 cut(s) 242, 266
HhaI GCGC 6 cut(s) 61, 517, 1231, 1450, 1512, 1539
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 130, 158, 227, 988, 1246
Hin6I GCGC 6 cut(s) 59, 515, 1229, 1448, 1510, 1537
HinP1I GCGC 6 cut(s) 59, 515, 1229, 1448, 1510, 1537
HincII GTYRAC 2 cut(s) 79, 1075
HindII GTYRAC 2 cut(s) 79, 1075
HinfI GANTC 4 cut(s) 146, 473, 1339, 1364
HpaII CCGG 3 cut(s) 210, 999, 1527
HphI GGTGA 3 cut(s) 121, 341, 988
Hpy166II GTNNAC 7 cut(s) 79, 654, 720, 927, 1075, 1301, 1543
Hpy188I TCNGA 5 cut(s) 69, 265, 286, 672, 1462
Hpy188III TCNNGA 3 cut(s) 985, 1327, 1382
Hpy8I GTNNAC 7 cut(s) 79, 654, 720, 927, 1075, 1301, 1543
Hpy99I CGWCG 3 cut(s) 10, 13, 260
HpyCH4III ACNGT 6 cut(s) 700, 766, 832, 1048, 1250, 1495
HpyCH4IV ACGT 1 cut(s) 1157
HpyCH4V TGCA 7 cut(s) 160, 227, 269, 383, 428, 629, 1031
HpyF3I CTNAG 7 cut(s) 363, 480, 534, 669, 933, 1257, 1451
HpySE526I ACGT 1 cut(s) 1157
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 130, 158, 227, 988, 1246
HspAI GCGC 6 cut(s) 59, 515, 1229, 1448, 1510, 1537
Kzo9I GATC 1 cut(s) 64
LmnI GCTCC 4 cut(s) 220, 328, 1284, 1504
LweI GCATC 7 cut(s) 256, 310, 529, 616, 904, 1168, 1309
MaeI CTAG 1 cut(s) 1182
MaeII ACGT 1 cut(s) 1157
MaeIII GTNAC 2 cut(s) 205, 1312
MalI GATC 1 cut(s) 66
MbiI CCGCTC 2 cut(s) 392, 1507
MboI GATC 1 cut(s) 64
MboII GAAGA 5 cut(s) 124, 412, 883, 1022, 1102
MhlI GDGCHC 3 cut(s) 984, 1274, 1493
MluCI AATT 2 cut(s) 724, 988
MlyI GAGTC 4 cut(s) 155, 482, 1333, 1373
MmeI TCCRAC 1 cut(s) 1485
MroXI GAANNNNTTC 2 cut(s) 17, 992
MslI CAYNNNNRTG 3 cut(s) 764, 962, 1383
MspA1I CMGCKG 3 cut(s) 104, 449, 1551
MspI CCGG 3 cut(s) 210, 999, 1527
MspR9I CCNGG 6 cut(s) 177, 327, 680, 1282, 1431, 1470
MvaI CCWGG 6 cut(s) 177, 327, 680, 1282, 1431, 1470
MvnI CGCG 6 cut(s) 59, 503, 1231, 1369, 1512, 1539
NdeII GATC 1 cut(s) 64
NlaIII CATG 5 cut(s) 130, 158, 227, 988, 1246
NmeAIII GCCGAG 3 cut(s) 61, 1066, 1165
NmuCI GTSAC 1 cut(s) 1312
NsbI TGCGCA 1 cut(s) 516
NspI RCATGY 2 cut(s) 130, 227
OliI CACNNNNGTG 1 cut(s) 764
PaeI GCATGC 1 cut(s) 227
PagI TCATGA 1 cut(s) 984
PasI CCCWGGG 1 cut(s) 1430
PciI ACATGT 1 cut(s) 126
PdmI GAANNNNTTC 2 cut(s) 17, 992
PinAI ACCGGT 1 cut(s) 998
PleI GAGTC 4 cut(s) 154, 481, 1333, 1372
PpsI GAGTC 4 cut(s) 154, 481, 1333, 1372
PscI ACATGT 1 cut(s) 126
Psp1406I AACGTT 1 cut(s) 1157
Psp6I CCWGG 6 cut(s) 175, 325, 678, 1280, 1429, 1468
PspFI CCCAGC 2 cut(s) 104, 644
PspGI CCWGG 6 cut(s) 175, 325, 678, 1280, 1429, 1468
PspPI GGNCC 3 cut(s) 676, 1246, 1543
PstI CTGCAG 2 cut(s) 385, 1033
PstNI CAGNNNCTG 1 cut(s) 1436
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 5 cut(s) 131, 500, 719, 1300, 1389
RsaNI GTAC 5 cut(s) 130, 499, 718, 1299, 1388
RseI CAYNNNNRTG 3 cut(s) 764, 962, 1383
SalI GTCGAC 1 cut(s) 1073
Sau3AI GATC 1 cut(s) 64
Sau96I GGNCC 3 cut(s) 676, 1246, 1543
SchI GAGTC 4 cut(s) 155, 482, 1333, 1373
ScrFI CCNGG 6 cut(s) 177, 327, 680, 1282, 1431, 1470
SduI GDGCHC 3 cut(s) 984, 1274, 1493
SetI ASST 8 cut(s) 106, 205, 634, 1160, 1222, 1338, 1393, 1404
SfaNI GCATC 7 cut(s) 256, 310, 529, 616, 904, 1168, 1309
SfcI CTRYAG 3 cut(s) 381, 828, 1029
SfiI GGCCNNNNNGGCC 1 cut(s) 683
SinI GGWCC 2 cut(s) 1246, 1543
SmiMI CAYNNNNRTG 3 cut(s) 764, 962, 1383
SphI GCATGC 1 cut(s) 227
Sse9I AATT 2 cut(s) 724, 988
SspMI CTAG 1 cut(s) 1182
StyD4I CCNGG 6 cut(s) 175, 325, 678, 1280, 1429, 1468
StyI CCWWGG 6 cut(s) 438, 468, 687, 744, 852, 1149
TaaI ACNGT 6 cut(s) 700, 766, 832, 1048, 1250, 1495
TaiI ACGT 1 cut(s) 1160
TaqI TCGA 3 cut(s) 11, 1074, 1410
TaqII GACCGA 1 cut(s) 1184
TasI AATT 2 cut(s) 724, 988
TatI WGTACW 2 cut(s) 717, 1298
TauI GCSGC 8 cut(s) 59, 395, 548, 1280, 1445, 1448, 1510, 1554
TscAI CASTG 6 cut(s) 222, 303, 670, 784, 1279, 1500
TseFI GTSAC 1 cut(s) 1312
Tsp45I GTSAC 1 cut(s) 1312
TspDTI ATGAA 4 cut(s) 428, 609, 1001, 1239
TspRI CASTG 6 cut(s) 222, 303, 670, 784, 1279, 1500
VpaK11BI GGWCC 2 cut(s) 1246, 1543
XceI RCATGY 2 cut(s) 130, 227
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmiI GTMKAC 3 cut(s) 653, 926, 1074
XmnI GAANNNNTTC 2 cut(s) 17, 992
XspI CTAG 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.