Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0012043

Physical Location & Seq

Genomic Coordinates Forward (+)

34178922 .. 34180838

Gene Structure 1917 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1267600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGGCGTCGTCGAAGCAAATGAAGGAGGAGACAGCTGCCAAGGCAGCAGCGAGGCTCGCTGCCGATGAACCGGCGGAAGAGACTGTCGTGGTGGAGACTACTTATGTGACTGATGACCAGGGGAGGCCAATAGAGTTTGCACAGCGCGAGGAGAAGCCCGGTGTTTTCGGGTCGATGATGAAGGCAGTGACGGGGACTTTAGAGCATGCCAAGGAAGCTGTGGTGGGGAAACCCCACGAAGAAAGTCAGGTTCAAGAGAAGGGCAAGGAGTATAAAGACTACACCACCCAGAAGGCGAAGGAGACCACTGATGCAGCCGGGGAGAAGATAAACGAGTATACTGAAACCGCTGAGGAGAAAGCGAAGCAGACCGCGGAGAAGGCAAATGAGACGAAGGAAGCGGCTAAGGGGAAGGTGGGTGAGTATGCGGATTACACAGCTGAAAAGGCGAAGGAAACTAAGGACACCGCTGCTCAGAAGGCGAACGAGGCTGCTCAAAAAGCGAAGGAGACAAAGGACAGCGCGGCTCAGAAGGCAAAGGAGGCGAAGGATGCAACAATGGGGAAAGCAGGCGAGTATACAAACTATGCAGCTGAGAAGGCAAAGGAGGCGAAGGACACAACTGTGAACAAAGCTGGCGAGTACACCAACTACACCGCCGAGAAGGCGAAGGAGGCCAAGGACACAACATTGAACAAGGCGAAGGAGGCGAAGGACACAACGATGAACAAAGCCGGCGAGTACACCAACTACGCCGCCGAGAAGGCGAAGCAGGCCAAGGACACTACGGTCAACAAAGCTGGTGAATATACCAACTATGCGGCTGAGAAGGCCAAGGAGGCTAAGGATGAGACGGTCAAGACAGCAAAGGAAACCAAGGACTGCACGGCTGAGAAGGCTAAAGAAGGGAAGGATACTACCGTGAGCAAGTTGGGTCAGCTAAAGGATACCGCTGCCGGTGCCGCTCAAAAGGCCATGGACTACTTGTCCGGCAAGAAGGAGGAGACCAAACAGAAGGCTGCCGAGACTGCCGAGAAAACAAAAGACACGACTTATGAGACTGCAGAGAAAGCGAAGGAGAAACTGAGTGAGACTGAGGAGGAAGCGAGGAGAAAGATGGAGCAGTTGAAGTTGCAAGGAAAAGAGTACAAAGATGAAGATGCTGACAGGGCACGTGAGGCACGTGACATCGAGGCCGAGAAAGGGCAGGCAGCGAAGGAAAACATATTCAGCTCGATTGGGAGCATTCCTGGTGCCATCAAAAGCAAGTTGACTCATCCTACGGATGTGGTGGAGGAGTCACGTGCCGCACGTGAGAGAGGGAGTACGGGGAGAAGGACCGTTCCTGTTGTGGAGGGGGTGGAGGTTGATGTGGCGGAGACGAGGCCTGGTGCCGTTGCAAGCAAGTTGTACGAGTCGGACCAGATGCATGGTCAAAACTTCAACGACGTGGGTCGTTTGGACGAGGATTGTAAAACGCAAGCGGAGATCCGCATTGATAACGAGGGCAAACGCGTGGAGCGCCTCCACAAAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0007275 GO:0008150 GO:0009791 GO:0009838 GO:0009908 GO:0010227 GO:0022414 GO:0032501 GO:0032502 GO:0044424 GO:0044444 GO:0044464 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LEA_4

Protein Analysis

513

Amino Acids

56.09

Weight (kDa)

5.62

Isoelectric Point (pI)

25.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LEA_4	PF02987	124 - 164	4.1e-11	Late embryogenesis abundant protein
LEA_4	PF02987	164 - 193	6.9e-09	Late embryogenesis abundant protein
LEA_4	PF02987	194 - 237	3.7e-17	Late embryogenesis abundant protein
LEA_4	PF02987	231 - 270	8.4e-17	Late embryogenesis abundant protein
LEA_4	PF02987	271 - 313	1.9e-15	Late embryogenesis abundant protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012043)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36640 AT2G36640 AT3G53040
fragaria_vesca	FvH4_6g17400
malus_domestica	MD09G1267600.v1.1
prunus_persica	Prupe.7G121500_v2.0.a1 Prupe.7G121500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0471651
rosa_laevigata	RLG00000024148
rosa_multiflora	Rmu_co8483845.1_g000001 Rmu_sc0005631.1_g000008
rosa_roxburghii	Rroxscaffold_6G00409690
rosa_rugosa	Rorug03G0118600
rosa_samantha	Rh3AG169800 Rh3BG195500 Rh3CG186200 Rh3CG195500 Rh3DG179500
rosa_wichuraiana	Rw0G008960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	788
AccB1I	GGYRCC	3 cut(s)	959, 1253, 1391
AccBSI	CCGCTC	1 cut(s)	965
AccI	GTMKAC	2 cut(s)	338, 578
AccII	CGCG	4 cut(s)	147, 374, 524, 1515
AclWI	GGATC	1 cut(s)	1483
AcvI	CACGTG	4 cut(s)	1175, 1184, 1304, 1313
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	5 cut(s)	644, 743, 1148, 1327, 1412
AfiI	CCNNNNNNNGG	1 cut(s)	1203
AflIII	ACRYGT	1 cut(s)	1513
AgsI	TTSAA	4 cut(s)	254, 694, 1129, 1444
AhdI	GACNNNNNGTC	1 cut(s)	985
AjiI	CACGTC	1 cut(s)	1450
AjnI	CCWGG	3 cut(s)	117, 1249, 1387
AleI	CACNNNNGTG	1 cut(s)	623
AluBI	AGCT	8 cut(s)	35, 218, 440, 593, 635, 800, 940, 1233
AluI	AGCT	8 cut(s)	35, 218, 440, 593, 635, 800, 940, 1233
AlwI	GGATC	1 cut(s)	1483
AoxI	GGCC	7 cut(s)	125, 675, 774, 831, 972, 1194, 1385
AspLEI	GCGC	3 cut(s)	147, 524, 1524
AspS9I	GGNCC	2 cut(s)	1338, 1420
AsuC2I	CCSGG	2 cut(s)	159, 319
AsuHPI	GGTGA	2 cut(s)	431, 815
AvaII	GGWCC	2 cut(s)	1338, 1420
BaeGI	GKGCMC	1 cut(s)	1174
BaeI	ACNNNNGTAYC	2 cut(s)	906, 939
BanI	GGYRCC	3 cut(s)	959, 1253, 1391
BbrPI	CACGTG	4 cut(s)	1175, 1184, 1304, 1313
BbvCI	CCTCAGC	1 cut(s)	351
BccI	CCATC	2 cut(s)	1111, 1265
BceAI	ACGGC	2 cut(s)	903, 1379
BciT130I	CCWGG	3 cut(s)	119, 1251, 1389
BciVI	GTATCC	2 cut(s)	907, 940
BcnI	CCSGG	2 cut(s)	159, 319
BfmI	CTRYAG	1 cut(s)	1062
BfoI	RGCGCY	1 cut(s)	1525
BfuI	GTATCC	2 cut(s)	907, 940
BglI	GCCNNNNNGGC	3 cut(s)	665, 764, 839
Bme1390I	CCNGG	5 cut(s)	119, 159, 319, 1251, 1389
Bme18I	GGWCC	2 cut(s)	1338, 1420
BmeRI	GACNNNNNGTC	1 cut(s)	985
BmgBI	CACGTC	1 cut(s)	1450
BmgT120I	GGNCC	2 cut(s)	1338, 1420
BmiI	GGNNCC	3 cut(s)	961, 1255, 1393
BmrFI	CCNGG	5 cut(s)	119, 159, 319, 1251, 1389
BmsI	GCATC	4 cut(s)	301, 541, 1150, 1416
BoxI	GACNNNNGTC	1 cut(s)	1452
Bpu10I	CCTNAGC	3 cut(s)	351, 405, 843
BpuMI	CCSGG	2 cut(s)	159, 319
BsaAI	YACGTR	4 cut(s)	1175, 1184, 1304, 1313
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	2 cut(s)	296, 998
Bsc4I	CCNNNNNNNGG	1 cut(s)	1203
Bse118I	RCCGGY	3 cut(s)	70, 734, 956
BseBI	CCWGG	3 cut(s)	119, 1251, 1389
BseGI	GGATG	4 cut(s)	556, 853, 1276, 1291
BseLI	CCNNNNNNNGG	1 cut(s)	1203
BseMII	CTCAG	8 cut(s)	342, 488, 542, 585, 816, 882, 1076, 1086
BseRI	GAGGAG	7 cut(s)	41, 164, 368, 1016, 1112, 1123, 1310
BseSI	GKGCMC	1 cut(s)	1174
BsgI	GTGCAG	1 cut(s)	868
Bsh1236I	CGCG	4 cut(s)	147, 374, 524, 1515
BshFI	GGCC	7 cut(s)	127, 677, 776, 833, 974, 1196, 1387
BshNI	GGYRCC	3 cut(s)	959, 1253, 1391
BsiSI	CCGG	6 cut(s)	71, 159, 318, 735, 957, 990
BslFI	GGGAC	1 cut(s)	208
BslI	CCNNNNNNNGG	1 cut(s)	1203
BsmBI	CGTCTC	3 cut(s)	383, 845, 1373
BsmFI	GGGAC	1 cut(s)	208
BsmI	GAATGC	1 cut(s)	1245
BsnI	GGCC	7 cut(s)	127, 677, 776, 833, 974, 1196, 1387
Bso31I	GGTCTC	2 cut(s)	296, 998
Bsp1286I	GDGCHC	1 cut(s)	1174
Bsp143I	GATC	1 cut(s)	1488
Bsp19I	CCATGG	1 cut(s)	975
BspANI	GGCC	7 cut(s)	127, 677, 776, 833, 974, 1196, 1387
BspCNI	CTCAG	8 cut(s)	343, 487, 541, 586, 817, 883, 1077, 1087
BspFNI	CGCG	4 cut(s)	147, 374, 524, 1515
BspLI	GGNNCC	3 cut(s)	961, 1255, 1393
BspMAI	CTGCAG	1 cut(s)	1066
BspPI	GGATC	1 cut(s)	1483
BspT107I	GGYRCC	3 cut(s)	959, 1253, 1391
BspTNI	GGTCTC	2 cut(s)	296, 998
BsrBI	CCGCTC	1 cut(s)	965
BsrFI	RCCGGY	3 cut(s)	70, 734, 956
BssAI	RCCGGY	3 cut(s)	70, 734, 956
BssMI	GATC	1 cut(s)	1488
BssNAI	GTATAC	2 cut(s)	339, 579
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	7 cut(s)	39, 210, 678, 777, 834, 876, 975
Bst1107I	GTATAC	2 cut(s)	339, 579
Bst2UI	CCWGG	3 cut(s)	119, 1251, 1389
Bst4CI	ACNGT	6 cut(s)	85, 625, 790, 856, 922, 1342
Bst6I	CTCTTC	1 cut(s)	72
BstACI	GRCGYC	1 cut(s)	5
BstBAI	YACGTR	4 cut(s)	1175, 1184, 1304, 1313
BstC8I	GCNNGC	9 cut(s)	57, 207, 571, 637, 736, 774, 1209, 1402, 1482
BstDSI	CCRYGG	2 cut(s)	372, 975
BstF5I	GGATG	4 cut(s)	556, 853, 1276, 1291
BstFNI	CGCG	4 cut(s)	147, 374, 524, 1515
BstH2I	RGCGCY	1 cut(s)	1525
BstHHI	GCGC	3 cut(s)	147, 524, 1524
BstKTI	GATC	1 cut(s)	1491
BstMBI	GATC	1 cut(s)	1488
BstNI	CCWGG	3 cut(s)	119, 1251, 1389
BstNSI	RCATGY	1 cut(s)	209
BstPAI	GACNNNNGTC	1 cut(s)	1452
BstSCI	CCNGG	5 cut(s)	117, 157, 317, 1249, 1387
BstSFI	CTRYAG	1 cut(s)	1062
BstSLI	GKGCMC	1 cut(s)	1174
BstUI	CGCG	4 cut(s)	147, 374, 524, 1515
BstX2I	RGATCY	1 cut(s)	1488
BstXI	CCANNNNNNTGG	1 cut(s)	1430
BstYI	RGATCY	1 cut(s)	1488
BstZ17I	GTATAC	2 cut(s)	339, 579
BsuI	GTATCC	2 cut(s)	907, 940
BsuRI	GGCC	7 cut(s)	127, 677, 776, 833, 974, 1196, 1387
BtgI	CCRYGG	2 cut(s)	372, 975
BtrI	CACGTC	1 cut(s)	1450
BtsCI	GGATG	4 cut(s)	556, 853, 1276, 1291
BtsI	GCAGTG	1 cut(s)	192
BtsIMutI	CAGTG	2 cut(s)	192, 306
Cac8I	GCNNGC	9 cut(s)	57, 207, 571, 637, 736, 774, 1209, 1402, 1482
CfoI	GCGC	3 cut(s)	147, 524, 1524
Cfr10I	RCCGGY	3 cut(s)	70, 734, 956
Cfr13I	GGNCC	2 cut(s)	1338, 1420
Cfr42I	CCGCGG	1 cut(s)	375
Csp6I	GTAC	5 cut(s)	643, 742, 1147, 1326, 1411
CviAII	CATG	3 cut(s)	206, 976, 1430
CviQI	GTAC	5 cut(s)	643, 742, 1147, 1326, 1411
DpnI	GATC	1 cut(s)	1490
DpnII	GATC	1 cut(s)	1488
DrdI	GACNNNNNNGTC	1 cut(s)	788
DriI	GACNNNNNGTC	1 cut(s)	985
DseDI	GACNNNNNNGTC	1 cut(s)	788
Eam1104I	CTCTTC	1 cut(s)	72
Eam1105I	GACNNNNNGTC	1 cut(s)	985
EarI	CTCTTC	1 cut(s)	72
EciI	GGCGGA	2 cut(s)	89, 1391
Eco130I	CCWWGG	7 cut(s)	39, 210, 678, 777, 834, 876, 975
Eco147I	AGGCCT	1 cut(s)	1387
Eco31I	GGTCTC	2 cut(s)	296, 998
Eco47I	GGWCC	2 cut(s)	1338, 1420
Eco72I	CACGTG	4 cut(s)	1175, 1184, 1304, 1313
EcoRII	CCWGG	3 cut(s)	117, 1249, 1387
EcoT14I	CCWWGG	7 cut(s)	39, 210, 678, 777, 834, 876, 975
EcoT22I	ATGCAT	1 cut(s)	1431
ErhI	CCWWGG	7 cut(s)	39, 210, 678, 777, 834, 876, 975
Esp3I	CGTCTC	3 cut(s)	383, 845, 1373
FaeI	CATG	3 cut(s)	209, 979, 1433
FaqI	GGGAC	1 cut(s)	208
FatI	CATG	3 cut(s)	205, 975, 1429
FblI	GTMKAC	2 cut(s)	338, 578
FokI	GGATG	4 cut(s)	563, 860, 1263, 1298
GlaI	GCGC	3 cut(s)	146, 523, 1523
HaeII	RGCGCY	1 cut(s)	1525
HaeIII	GGCC	7 cut(s)	127, 677, 776, 833, 974, 1196, 1387
HapII	CCGG	6 cut(s)	71, 159, 318, 735, 957, 990
HhaI	GCGC	3 cut(s)	147, 524, 1524
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	3 cut(s)	209, 979, 1433
Hin6I	GCGC	3 cut(s)	145, 522, 1522
HinP1I	GCGC	3 cut(s)	145, 522, 1522
HincII	GTYRAC	2 cut(s)	793, 1272
HindII	GTYRAC	2 cut(s)	793, 1272
HinfI	GANTC	3 cut(s)	1273, 1298, 1415
HpaII	CCGG	6 cut(s)	71, 159, 318, 735, 957, 990
HphI	GGTGA	2 cut(s)	431, 815
Hpy166II	GTNNAC	7 cut(s)	339, 579, 628, 645, 744, 793, 1272
Hpy188I	TCNGA	3 cut(s)	477, 531, 1420
Hpy188III	TCNNGA	2 cut(s)	254, 859
Hpy8I	GTNNAC	7 cut(s)	339, 579, 628, 645, 744, 793, 1272
Hpy99I	CGWCG	3 cut(s)	10, 13, 1451
HpyCH4III	ACNGT	6 cut(s)	85, 625, 790, 856, 922, 1342
HpyCH4IV	ACGT	5 cut(s)	1174, 1183, 1303, 1312, 1449
HpyCH4V	TGCA	9 cut(s)	140, 314, 554, 590, 885, 1064, 1135, 1400, 1429
HpySE526I	ACGT	5 cut(s)	1174, 1183, 1303, 1312, 1449
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	3 cut(s)	209, 979, 1433
HspAI	GCGC	3 cut(s)	145, 522, 1522
KroI	GCCGGC	1 cut(s)	734
KroNI	GCCGGC	1 cut(s)	736
KspI	CCGCGG	1 cut(s)	375
Kzo9I	GATC	1 cut(s)	1488
LmnI	GCTCC	3 cut(s)	1120, 1242, 1519
LweI	GCATC	4 cut(s)	301, 541, 1150, 1416
MaeII	ACGT	5 cut(s)	1174, 1183, 1303, 1312, 1449
MaeIII	GTNAC	4 cut(s)	106, 187, 1184, 1299
MalI	GATC	1 cut(s)	1490
MbiI	CCGCTC	1 cut(s)	965
MboI	GATC	1 cut(s)	1488
MboII	GAAGA	4 cut(s)	89, 251, 337, 1169
MflI	RGATCY	1 cut(s)	1488
MhlI	GDGCHC	1 cut(s)	1174
MluI	ACGCGT	1 cut(s)	1513
MlyI	GAGTC	3 cut(s)	1267, 1307, 1424
MmeI	TCCRAC	1 cut(s)	1398
Mph1103I	ATGCAT	1 cut(s)	1431
MroNI	GCCGGC	1 cut(s)	734
MslI	CAYNNNNRTG	2 cut(s)	623, 722
MspA1I	CMGCKG	7 cut(s)	35, 350, 374, 440, 470, 593, 953
MspI	CCGG	6 cut(s)	71, 159, 318, 735, 957, 990
MspR9I	CCNGG	5 cut(s)	119, 159, 319, 1251, 1389
Mva1269I	GAATGC	1 cut(s)	1245
MvaI	CCWGG	3 cut(s)	119, 1251, 1389
MvnI	CGCG	4 cut(s)	147, 374, 524, 1515
NaeI	GCCGGC	1 cut(s)	736
NciI	CCSGG	2 cut(s)	159, 319
NcoI	CCATGG	1 cut(s)	975
NdeII	GATC	1 cut(s)	1488
NgoMIV	GCCGGC	1 cut(s)	734
NlaIII	CATG	3 cut(s)	209, 979, 1433
NlaIV	GGNNCC	3 cut(s)	961, 1255, 1393
NmeAIII	GCCGAG	5 cut(s)	685, 784, 1048, 1057, 1222
NmuCI	GTSAC	4 cut(s)	106, 187, 1184, 1299
NsiI	ATGCAT	1 cut(s)	1431
NspI	RCATGY	1 cut(s)	209
OliI	CACNNNNGTG	1 cut(s)	623
PaeI	GCATGC	1 cut(s)	209
PceI	AGGCCT	1 cut(s)	1387
PcsI	WCGNNNNNNNCGW	2 cut(s)	1180, 1309
PctI	GAATGC	1 cut(s)	1245
PdiI	GCCGGC	1 cut(s)	736
PleI	GAGTC	3 cut(s)	1267, 1306, 1423
PmaCI	CACGTG	4 cut(s)	1175, 1184, 1304, 1313
PmlI	CACGTG	4 cut(s)	1175, 1184, 1304, 1313
PpsI	GAGTC	3 cut(s)	1267, 1306, 1423
Ppu21I	YACGTR	4 cut(s)	1175, 1184, 1304, 1313
PshAI	GACNNNNGTC	1 cut(s)	1452
Psp6I	CCWGG	3 cut(s)	117, 1249, 1387
PspCI	CACGTG	4 cut(s)	1175, 1184, 1304, 1313
PspGI	CCWGG	3 cut(s)	117, 1249, 1387
PspN4I	GGNNCC	3 cut(s)	961, 1255, 1393
PspPI	GGNCC	2 cut(s)	1338, 1420
PstI	CTGCAG	1 cut(s)	1066
PsuI	RGATCY	1 cut(s)	1488
PvuII	CAGCTG	3 cut(s)	35, 440, 593
RsaI	GTAC	5 cut(s)	644, 743, 1148, 1327, 1412
RsaNI	GTAC	5 cut(s)	643, 742, 1147, 1326, 1411
RseI	CAYNNNNRTG	2 cut(s)	623, 722
SacII	CCGCGG	1 cut(s)	375
Sau3AI	GATC	1 cut(s)	1488
Sau96I	GGNCC	2 cut(s)	1338, 1420
SchI	GAGTC	3 cut(s)	1267, 1307, 1424
ScrFI	CCNGG	5 cut(s)	119, 159, 319, 1251, 1389
SduI	GDGCHC	1 cut(s)	1174
SfaNI	GCATC	4 cut(s)	301, 541, 1150, 1416
SfcI	CTRYAG	1 cut(s)	1062
Sfr303I	CCGCGG	1 cut(s)	375
SgrBI	CCGCGG	1 cut(s)	375
SinI	GGWCC	2 cut(s)	1338, 1420
SmiMI	CAYNNNNRTG	2 cut(s)	623, 722
SphI	GCATGC	1 cut(s)	209
SseBI	AGGCCT	1 cut(s)	1387
StuI	AGGCCT	1 cut(s)	1387
StyD4I	CCNGG	5 cut(s)	117, 157, 317, 1249, 1387
StyI	CCWWGG	7 cut(s)	39, 210, 678, 777, 834, 876, 975
TaaI	ACNGT	6 cut(s)	85, 625, 790, 856, 922, 1342
TaiI	ACGT	5 cut(s)	1177, 1186, 1306, 1315, 1452
TaqI	TCGA	4 cut(s)	11, 173, 1191, 1235
TatI	WGTACW	3 cut(s)	642, 741, 1146
TauI	GCSGC	6 cut(s)	404, 527, 758, 824, 965, 1310
TscAI	CASTG	2 cut(s)	192, 313
TseFI	GTSAC	4 cut(s)	106, 187, 1184, 1299
Tsp45I	GTSAC	4 cut(s)	106, 187, 1184, 1299
TspDTI	ATGAA	5 cut(s)	35, 81, 194, 740, 1170
TspGWI	ACGGA	1 cut(s)	1298
TspRI	CASTG	2 cut(s)	192, 313
VpaK11BI	GGWCC	2 cut(s)	1338, 1420
XceI	RCATGY	1 cut(s)	209
XcmI	CCANNNNNNNNNTGG	1 cut(s)	217
XmiI	GTMKAC	2 cut(s)	338, 578
Zsp2I	ATGCAT	1 cut(s)	1431

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.