AT3G53040

embryonic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012043
Physical Location & Seq
Reverse (-)
19664629 .. 19666497
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G53040.1

Sequence Viewer

Length: 1440 bp
ATGGCATCAGGACAACGGGAGGCAGAGAGGTCCGCCAAGGCCGAGAGAGCTGAGGCTGCAGCGAGTCTAGCGGCTGAAGATTTGAAAGATATCAACAAAGGTGATGTGACGTACAAATTAACGGAGAGGACAACGACGACCGAGCCTGAGAGGCCTGGCCTGATAGGCTCGGTGATGAAGGCGGTGCAAGGAACTAAAGACGCAGTCATCGGTAAAAGTCATGATACGGCTGAGTCAACGAGGGAGGGAGCCGATATTGCATCGGAGAAGGCAGCGGGGATGAGAGACACCACCGGAGAGGTTAGAGACAGTACTGCGCAGAAGACTAAGGAAACTGCAGATTACACGGCCGATAAGGCAAGAGAAGCTAAAGATAAGACTGCGGACAAGACTAAGGAAACCGCAGATTACGCGGCAGAGAAGGCAAGAGAAGCTAAAGATAGAACGGCAGACAAGACTAAGGAAACGGCAGAATACACGGCGGAGAAGGCAAGAGAAGCTAAAGATAAAACGGCGGATAAGTTGGGTGAATATAAAGATTACACGGCAGAGAAGGCAAAGGAAGCCAAAGACACGACGGCGGAGAAGCTGGGTGAGTACAAAGACTACACGGTGGATAAGGCGAAAGAAGCTAAGGATAAGACGGCAGAGAAAGCAAAGGAAACCGCGGAATACACCTCGGATAAGGCGAGGGAAACAAAAGATAAGACAGCTGAGAAAGTGGGCGAATATAAAGACTACACGGCGGAGAAAGCAAAGGAAACTGCGGATAAGGCGAGGGAAGCAAAAGATAAGACAGCTGAGAAAGTGGGAGAATATAGAGACTACACGGCAGAGAAGGCGACAGAAACGAAAGATGCAGGTGTGAGTAAGATCGGAGAACTGAAGGATAGTGCAGTGGACACTGCTAAAAGAGCTATGGGTTTCTTGTCGGGCAAGACAGAGGAAACCAAACAAAAAGCCGTTGAGACCAAAGACACGGCCAAGGAAAAGATGGACGAGGCGGGAGAAGAAGCAAGGCGGAAGATGGAAGAGATGAGACTTGAGGGCAAAAAACTTGACGAGGATGCTTCTAGAAAGACACAACAGAGTACCGAATCTGCTGCTGACAAAGCTCACGAGACTAAAGATTCCGTAGCCCAAAGAGGTGAAGAAGGGAAGGGAAGTATAATGGGTGCGTTGGGGAACATGACTGGAGCTATAAAGAGCAAGCTGACTGGTGCTACGACGCCCTCTGATGAGGAGACGCGCGCGTCGGCCCATGGAGACGAGAGCACAGGGAAGACAGTTGTGGCGGTTGACGTGAAAGATACTAGGCCTGGATACGTGGCGACGGTACTGAAAGAGGCGGATCAGATGACCGGTCAAACTTTCAATGACGTTGGAGAGATCGATGACGAGGAGAAAGTGAGAATCGTCGTGGGAGAGAAAAAGCTGTAG

Protein Analysis

479

Amino Acids

52.08

Weight (kDa)

5.29

Isoelectric Point (pI)

11.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_4 PF02987 96 - 134 1.3e-07 Late embryogenesis abundant protein
LEA_4 PF02987 135 - 178 3.5e-14 Late embryogenesis abundant protein
LEA_4 PF02987 179 - 222 1.2e-17 Late embryogenesis abundant protein
LEA_4 PF02987 224 - 255 3e-12 Late embryogenesis abundant protein
LEA_4 PF02987 255 - 295 6e-16 Late embryogenesis abundant protein
LEA_4 PF02987 275 - 306 3e-07 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012043)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36640 AT2G36640 AT3G53040
fragaria_vesca FvH4_6g17400
malus_domestica MD09G1267600.v1.1
prunus_persica Prupe.7G121500_v2.0.a1 Prupe.7G121500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0471651
rosa_laevigata RLG00000024148
rosa_multiflora Rmu_co8483845.1_g000001 Rmu_sc0005631.1_g000008
rosa_roxburghii Rroxscaffold_6G00409690
rosa_rugosa Rorug03G0118600
rosa_samantha Rh3AG169800 Rh3BG195500 Rh3CG186200 Rh3CG195500 Rh3DG179500
rosa_wichuraiana Rw0G008960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 851
AasI GACNNNNNNGTC 1 cut(s) 1252
Acc16I TGCGCA 1 cut(s) 318
Acc36I ACCTGC 1 cut(s) 851
AccII CGCG 5 cut(s) 413, 668, 1249, 1251, 1253
AclWI GGATC 1 cut(s) 1359
AcoI YGGCCR 2 cut(s) 348, 981
AcuI CTGAAG 2 cut(s) 96, 905
AcyI GRCGYC 1 cut(s) 1229
AfaI GTAC 5 cut(s) 113, 313, 599, 1093, 1338
AgeI ACCGGT 1 cut(s) 1361
AgsI TTSAA 2 cut(s) 85, 1375
AjiI CACGTC 1 cut(s) 1303
AjnI CCWGG 2 cut(s) 154, 1318
Alw21I GWGCWC 1 cut(s) 1277
Alw26I GTCTC 8 cut(s) 279, 300, 816, 962, 1033, 1115, 1238, 1260
AlwI GGATC 1 cut(s) 1359
AoxI GGCC 7 cut(s) 39, 152, 157, 348, 981, 1257, 1316
ApeKI GCWGC 4 cut(s) 56, 59, 272, 1103
AsiGI ACCGGT 1 cut(s) 1361
AspLEI GCGC 3 cut(s) 319, 1251, 1253
AspS9I GGNCC 2 cut(s) 30, 1258
AsuHPI GGTGA 5 cut(s) 113, 184, 539, 605, 1160
AvaII GGWCC 1 cut(s) 30
BauI CACGAG 1 cut(s) 1118
BbsI GAAGAC 2 cut(s) 329, 1289
Bbv12I GWGCWC 1 cut(s) 1277
BbvCI CCTCAGC 1 cut(s) 51
BbvI GCAGC 4 cut(s) 43, 71, 284, 1090
BccI CCATC 2 cut(s) 988, 1021
BcgI CGANNNNNNTGC 2 cut(s) 1085, 1119
BciT130I CCWGG 2 cut(s) 156, 1320
BciVI GTATCC 1 cut(s) 1316
BcoDI GTCTC 8 cut(s) 279, 300, 816, 962, 1033, 1115, 1238, 1260
BfaI CTAG 3 cut(s) 68, 1074, 1314
BfmI CTRYAG 3 cut(s) 57, 336, 1436
BfuAI ACCTGC 1 cut(s) 851
BfuI GTATCC 1 cut(s) 1316
BglI GCCNNNNNGGC 3 cut(s) 151, 165, 356
BisI GCNGC 6 cut(s) 57, 60, 72, 273, 414, 1104
BlsI GCNGC 6 cut(s) 58, 61, 73, 274, 415, 1105
BmcAI AGTACT 1 cut(s) 313
Bme1390I CCNGG 2 cut(s) 156, 1320
Bme18I GGWCC 1 cut(s) 30
BmgBI CACGTC 1 cut(s) 1303
BmgT120I GGNCC 2 cut(s) 30, 1258
BmiI GGNNCC 1 cut(s) 250
BmrFI CCNGG 2 cut(s) 156, 1320
BmsI GCATC 4 cut(s) 14, 269, 847, 1057
BpiI GAAGAC 2 cut(s) 329, 1289
BpmI CTGGAG 1 cut(s) 1215
Bpu10I CCTNAGC 2 cut(s) 51, 633
BpuEI CTTGAG 1 cut(s) 1064
Bsa29I ATCGAT 1 cut(s) 1392
BsaAI YACGTR 1 cut(s) 1327
BsaHI GRCGYC 1 cut(s) 1229
BsaI GGTCTC 1 cut(s) 962
BsaJI CCNNGG 5 cut(s) 36, 666, 678, 984, 1261
BsaWI WCCGGW 2 cut(s) 293, 1361
Bse118I RCCGGY 1 cut(s) 1361
Bse1I ACTGG 2 cut(s) 1198, 1222
BseBI CCWGG 2 cut(s) 156, 1320
BseCI ATCGAT 1 cut(s) 1392
BseDI CCNNGG 5 cut(s) 36, 666, 678, 984, 1261
BseGI GGATG 2 cut(s) 285, 1072
BseMII CTCAG 5 cut(s) 42, 138, 222, 705, 792
BseNI ACTGG 2 cut(s) 1198, 1222
BsePI GCGCGC 1 cut(s) 1249
BseRI GAGGAG 2 cut(s) 1256, 1415
BseX3I CGGCCG 1 cut(s) 348
BseXI GCAGC 4 cut(s) 43, 71, 284, 1090
BseYI CCCAGC 1 cut(s) 589
BsgI GTGCAG 1 cut(s) 915
Bsh1236I CGCG 5 cut(s) 413, 668, 1249, 1251, 1253
Bsh1285I CGRYCG 2 cut(s) 141, 351
BshFI GGCC 7 cut(s) 41, 154, 159, 350, 983, 1259, 1318
BshTI ACCGGT 1 cut(s) 1361
BshVI ATCGAT 1 cut(s) 1392
BsiEI CGRYCG 2 cut(s) 141, 351
BsiHKAI GWGCWC 1 cut(s) 1277
BsiSI CCGG 2 cut(s) 294, 1362
BsmAI GTCTC 8 cut(s) 279, 300, 816, 962, 1033, 1115, 1238, 1260
BsmBI CGTCTC 2 cut(s) 1238, 1260
BsnI GGCC 7 cut(s) 41, 154, 159, 350, 983, 1259, 1318
Bso31I GGTCTC 1 cut(s) 962
Bsp1286I GDGCHC 1 cut(s) 1277
Bsp143I GATC 3 cut(s) 873, 1351, 1389
Bsp19I CCATGG 1 cut(s) 1261
BspANI GGCC 7 cut(s) 41, 154, 159, 350, 983, 1259, 1318
BspCNI CTCAG 5 cut(s) 43, 139, 223, 706, 793
BspDI ATCGAT 1 cut(s) 1392
BspFNI CGCG 5 cut(s) 413, 668, 1249, 1251, 1253
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 1 cut(s) 250
BspMAI CTGCAG 2 cut(s) 61, 340
BspMI ACCTGC 1 cut(s) 851
BspPI GGATC 1 cut(s) 1359
BspTNI GGTCTC 1 cut(s) 962
BsrFI RCCGGY 1 cut(s) 1361
BsrI ACTGG 2 cut(s) 1198, 1222
BssAI RCCGGY 1 cut(s) 1361
BssECI CCNNGG 5 cut(s) 36, 666, 678, 984, 1261
BssHII GCGCGC 1 cut(s) 1249
BssMI GATC 3 cut(s) 873, 1351, 1389
BssNI GRCGYC 1 cut(s) 1229
BssSI CACGAG 1 cut(s) 1118
BssT1I CCWWGG 3 cut(s) 36, 984, 1261
Bst2BI CACGAG 1 cut(s) 1118
Bst2UI CCWGG 2 cut(s) 156, 1320
Bst4CI ACNGT 4 cut(s) 311, 613, 1288, 1336
Bst6I CTCTTC 1 cut(s) 1026
BstACI GRCGYC 1 cut(s) 1229
BstBAI YACGTR 1 cut(s) 1327
BstC8I GCNNGC 2 cut(s) 1211, 1251
BstDEI CTNAG 9 cut(s) 51, 147, 231, 327, 393, 459, 633, 714, 801
BstDSI CCRYGG 2 cut(s) 666, 1261
BstF5I GGATG 2 cut(s) 285, 1072
BstFNI CGCG 5 cut(s) 413, 668, 1249, 1251, 1253
BstHHI GCGC 3 cut(s) 319, 1251, 1253
BstKTI GATC 3 cut(s) 876, 1354, 1392
BstMAI GTCTC 8 cut(s) 279, 300, 816, 962, 1033, 1115, 1238, 1260
BstMBI GATC 3 cut(s) 873, 1351, 1389
BstMCI CGRYCG 2 cut(s) 141, 351
BstNI CCWGG 2 cut(s) 156, 1320
BstSCI CCNGG 2 cut(s) 154, 1318
BstSFI CTRYAG 3 cut(s) 57, 336, 1436
BstUI CGCG 5 cut(s) 413, 668, 1249, 1251, 1253
BstV1I GCAGC 4 cut(s) 43, 71, 284, 1090
BstV2I GAAGAC 2 cut(s) 329, 1289
BstZI CGGCCG 1 cut(s) 348
Bsu15I ATCGAT 1 cut(s) 1392
BsuI GTATCC 1 cut(s) 1316
BsuRI GGCC 7 cut(s) 41, 154, 159, 350, 983, 1259, 1318
BsuTUI ATCGAT 1 cut(s) 1392
BtgI CCRYGG 2 cut(s) 666, 1261
BtrI CACGTC 1 cut(s) 1303
BtsCI GGATG 2 cut(s) 285, 1072
BtsI GCAGTG 2 cut(s) 903, 903
BtsIMutI CAGTG 2 cut(s) 903, 903
BveI ACCTGC 1 cut(s) 851
Cac8I GCNNGC 2 cut(s) 1211, 1251
CciI TCATGA 1 cut(s) 220
CfoI GCGC 3 cut(s) 319, 1251, 1253
Cfr10I RCCGGY 1 cut(s) 1361
Cfr13I GGNCC 2 cut(s) 30, 1258
Cfr42I CCGCGG 1 cut(s) 669
ClaI ATCGAT 1 cut(s) 1392
CseI GACGC 4 cut(s) 209, 1237, 1242, 1255
Csp6I GTAC 5 cut(s) 112, 312, 598, 1092, 1337
CspAI ACCGGT 1 cut(s) 1361
CviAII CATG 3 cut(s) 221, 1189, 1262
CviQI GTAC 5 cut(s) 112, 312, 598, 1092, 1337
DdeI CTNAG 9 cut(s) 51, 147, 231, 327, 393, 459, 633, 714, 801
DpnI GATC 3 cut(s) 875, 1353, 1391
DpnII GATC 3 cut(s) 873, 1351, 1389
DrdI GACNNNNNNGTC 1 cut(s) 1252
DseDI GACNNNNNNGTC 1 cut(s) 1252
EaeI YGGCCR 2 cut(s) 348, 981
EagI CGGCCG 1 cut(s) 348
Eam1104I CTCTTC 1 cut(s) 1026
EarI CTCTTC 1 cut(s) 1026
EciI GGCGGA 7 cut(s) 22, 497, 530, 596, 761, 1036, 1364
EclXI CGGCCG 1 cut(s) 348
Eco130I CCWWGG 3 cut(s) 36, 984, 1261
Eco147I AGGCCT 2 cut(s) 154, 1318
Eco31I GGTCTC 1 cut(s) 962
Eco32I GATATC 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 30
Eco52I CGGCCG 1 cut(s) 348
Eco57I CTGAAG 2 cut(s) 96, 905
EcoRII CCWGG 2 cut(s) 154, 1318
EcoRV GATATC 1 cut(s) 91
EcoT14I CCWWGG 3 cut(s) 36, 984, 1261
ErhI CCWWGG 3 cut(s) 36, 984, 1261
Esp3I CGTCTC 2 cut(s) 1238, 1260
FaeI CATG 3 cut(s) 224, 1192, 1265
FaiI YATR 9 cut(s) 222, 534, 732, 819, 920, 1169, 1190, 1202, 1263
FatI CATG 3 cut(s) 220, 1188, 1261
FauI CCCGC 2 cut(s) 268, 997
Fnu4HI GCNGC 6 cut(s) 57, 60, 72, 273, 414, 1104
FokI GGATG 2 cut(s) 292, 1079
Fsp4HI GCNGC 6 cut(s) 57, 60, 72, 273, 414, 1104
FspBI CTAG 3 cut(s) 68, 1074, 1314
FspI TGCGCA 1 cut(s) 318
GlaI GCGC 3 cut(s) 318, 1250, 1252
GluI GCNGC 6 cut(s) 57, 60, 72, 273, 414, 1104
GsaI CCCAGC 1 cut(s) 593
GsuI CTGGAG 1 cut(s) 1215
HaeIII GGCC 7 cut(s) 41, 154, 159, 350, 983, 1259, 1318
HapII CCGG 2 cut(s) 294, 1362
HgaI GACGC 4 cut(s) 209, 1237, 1242, 1255
HhaI GCGC 3 cut(s) 319, 1251, 1253
Hin1I GRCGYC 1 cut(s) 1229
Hin1II CATG 3 cut(s) 224, 1192, 1265
Hin6I GCGC 3 cut(s) 317, 1249, 1251
HinP1I GCGC 3 cut(s) 317, 1249, 1251
HincII GTYRAC 2 cut(s) 237, 1300
HindII GTYRAC 2 cut(s) 237, 1300
HinfI GANTC 5 cut(s) 64, 233, 1097, 1130, 1413
HpaII CCGG 2 cut(s) 294, 1362
HphI GGTGA 5 cut(s) 113, 184, 539, 605, 1160
Hpy166II GTNNAC 3 cut(s) 237, 901, 1300
Hpy188I TCNGA 5 cut(s) 265, 682, 878, 1237, 1356
Hpy188III TCNNGA 4 cut(s) 9, 221, 1074, 1118
Hpy8I GTNNAC 3 cut(s) 237, 901, 1300
Hpy99I CGWCG 6 cut(s) 139, 580, 1231, 1258, 1336, 1421
HpyAV CCTTC 9 cut(s) 172, 262, 415, 481, 547, 832, 880, 1148, 1153
HpyCH4III ACNGT 4 cut(s) 311, 613, 1288, 1336
HpyCH4IV ACGT 4 cut(s) 110, 1302, 1326, 1380
HpyCH4V TGCA 6 cut(s) 59, 187, 260, 338, 860, 896
HpyF3I CTNAG 9 cut(s) 51, 147, 231, 327, 393, 459, 633, 714, 801
HpySE526I ACGT 4 cut(s) 110, 1302, 1326, 1380
Hsp92I GRCGYC 1 cut(s) 1229
Hsp92II CATG 3 cut(s) 224, 1192, 1265
HspAI GCGC 3 cut(s) 317, 1249, 1251
KspI CCGCGG 1 cut(s) 669
Kzo9I GATC 3 cut(s) 873, 1351, 1389
LmnI GCTCC 2 cut(s) 248, 1196
Lsp1109I GCAGC 4 cut(s) 43, 71, 284, 1090
LweI GCATC 4 cut(s) 14, 269, 847, 1057
MaeI CTAG 3 cut(s) 68, 1074, 1314
MaeII ACGT 4 cut(s) 110, 1302, 1326, 1380
MaeIII GTNAC 1 cut(s) 106
MalI GATC 3 cut(s) 875, 1353, 1391
MauBI CGCGCGCG 1 cut(s) 1249
MboI GATC 3 cut(s) 873, 1351, 1389
MboII GAAGA 7 cut(s) 89, 334, 1022, 1036, 1043, 1163, 1294
MhlI GDGCHC 1 cut(s) 1277
MluCI AATT 1 cut(s) 116
MlyI GAGTC 2 cut(s) 73, 242
MmeI TCCRAC 1 cut(s) 1363
MseI TTAA 1 cut(s) 119
MspA1I CMGCKG 4 cut(s) 275, 668, 713, 800
MspI CCGG 2 cut(s) 294, 1362
MspR9I CCNGG 2 cut(s) 156, 1320
MvaI CCWGG 2 cut(s) 156, 1320
MvnI CGCG 5 cut(s) 413, 668, 1249, 1251, 1253
NcoI CCATGG 1 cut(s) 1261
NdeII GATC 3 cut(s) 873, 1351, 1389
NlaIII CATG 3 cut(s) 224, 1192, 1265
NlaIV GGNNCC 1 cut(s) 250
NmeAIII GCCGAG 1 cut(s) 67
NmuCI GTSAC 1 cut(s) 106
NsbI TGCGCA 1 cut(s) 318
PagI TCATGA 1 cut(s) 220
PaqCI CACCTGC 1 cut(s) 851
PauI GCGCGC 1 cut(s) 1249
PceI AGGCCT 2 cut(s) 154, 1318
PcsI WCGNNNNNNNCGW 1 cut(s) 686
PfeI GAWTC 3 cut(s) 1097, 1130, 1413
PflFI GACNNNGTC 1 cut(s) 203
PinAI ACCGGT 1 cut(s) 1361
PkrI GCNGC 6 cut(s) 58, 61, 73, 274, 415, 1105
PleI GAGTC 2 cut(s) 72, 241
PpsI GAGTC 2 cut(s) 72, 241
Ppu21I YACGTR 1 cut(s) 1327
Psp6I CCWGG 2 cut(s) 154, 1318
PspFI CCCAGC 1 cut(s) 589
PspGI CCWGG 2 cut(s) 154, 1318
PspN4I GGNNCC 1 cut(s) 250
PspPI GGNCC 2 cut(s) 30, 1258
PstI CTGCAG 2 cut(s) 61, 340
PsyI GACNNNGTC 1 cut(s) 203
PteI GCGCGC 1 cut(s) 1249
PvuII CAGCTG 2 cut(s) 713, 800
RsaI GTAC 5 cut(s) 113, 313, 599, 1093, 1338
RsaNI GTAC 5 cut(s) 112, 312, 598, 1092, 1337
SacII CCGCGG 1 cut(s) 669
SaqAI TTAA 1 cut(s) 119
SatI GCNGC 6 cut(s) 57, 60, 72, 273, 414, 1104
Sau3AI GATC 3 cut(s) 873, 1351, 1389
Sau96I GGNCC 2 cut(s) 30, 1258
ScaI AGTACT 1 cut(s) 313
SchI GAGTC 2 cut(s) 73, 242
ScrFI CCNGG 2 cut(s) 156, 1320
SduI GDGCHC 1 cut(s) 1277
SfaNI GCATC 4 cut(s) 14, 269, 847, 1057
SfcI CTRYAG 3 cut(s) 57, 336, 1436
Sfr303I CCGCGG 1 cut(s) 669
SgrBI CCGCGG 1 cut(s) 669
SinI GGWCC 1 cut(s) 30
SmlI CTYRAG 1 cut(s) 1043
SmoI CTYRAG 1 cut(s) 1043
Sse9I AATT 1 cut(s) 116
SseBI AGGCCT 2 cut(s) 154, 1318
SspMI CTAG 3 cut(s) 68, 1074, 1314
StuI AGGCCT 2 cut(s) 154, 1318
StyD4I CCNGG 2 cut(s) 154, 1318
StyI CCWWGG 3 cut(s) 36, 984, 1261
TaaI ACNGT 4 cut(s) 311, 613, 1288, 1336
TaiI ACGT 4 cut(s) 113, 1305, 1329, 1383
TaqI TCGA 1 cut(s) 1392
TaqII GACCGA 1 cut(s) 155
TasI AATT 1 cut(s) 116
TatI WGTACW 2 cut(s) 311, 597
TauI GCSGC 2 cut(s) 74, 416
TfiI GAWTC 3 cut(s) 1097, 1130, 1413
Tru1I TTAA 1 cut(s) 119
Tru9I TTAA 1 cut(s) 119
TscAI CASTG 2 cut(s) 903, 910
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 4 cut(s) 56, 59, 272, 1103
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 1 cut(s) 191
TspGWI ACGGA 2 cut(s) 137, 1123
TspRI CASTG 2 cut(s) 903, 910
Tth111I GACNNNGTC 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 30
XbaI TCTAGA 1 cut(s) 1073
XcmI CCANNNNNNNNNTGG 1 cut(s) 991
XspI CTAG 3 cut(s) 68, 1074, 1314
ZrmI AGTACT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.