Prupe.7G121500_v2.0.a1

Embryonic protein DC-8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0012043
Physical Location & Seq
Reverse (-)
14467931 .. 14469921
1991 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G121500.2

Sequence Viewer

Length: 1476 bp
ATGGCGTCGAGGCAAGCCAAGGAGAAGAGAGCCGAGGCCGCAGCAAGGCTCGCAGCGGATGAGTTGAGAGACGTGAACAAAGGCCAAGTCTATGAAGAAAAAACAAAGATTGAGACTACTTATACGCCTAACGATGAGCTGGGGAGGCCAGCAGAGCAAGTGCATGAAGAGAAGCCTGGGGTGATCGGGTCGGTGCTGAAGACGGTGACCGGGAAGCTAGAGCAAGCGAAGGATGCTGTGGTGGGGAAAAGCCACGATGAAAGTCATGTTGAAGAGAAGGCCAAAGAGACCACGGGCGCCGCGGGGGAGAAGATAAATGAGTACACGGGTACCGCGGCTGAGAAGGCGAAACAGTCAAAGGACACGGCAGCTCAAAAAGCCAAGGACGCAAAGGACACGGCAGCTCAAAAAGCCAAGGACGCAAAGGACACAACAATGGGGAAAACAAGTGAGTATACTGGTTATGCAGCACAGAAGGCCAAGGAGACTAAGGACGCAGCAGCTCAAGAGGCCAAGGAGGCAAAGGACACGACAATGGGGAAAACAAGCGATTATACAGGTTATGCTGCTGACAAGACAAAAGAGACCAAGGATTATGCTGCTGAAAAGGCTAAGGAGGCCGCCGAGAAGGCGAAGGAGACGGAAGACTCGGCAATGGGGAAAGCTGGTGAGTATACAAACTATGCCACTGAGAAAGCAAAGGAGGCAAAAGACGCAACAATGCAGAAAGCAAAGGATGCTAAAGACACGACTGTTGGGAAAGCAAGCGAGTATACAAATTATGCTGCGGAGAAGGCCAAGGAGGCCAAAGACACAACAGCCGAGAAGGCCAATGAGACCAAGGACTACACAGATGAGAAAGCAAAAGAAGGGAAGGACACGACTGTGAGTAAGTTGGGCGAGCTGAAGGACACGGCTGCCGGCGCTGCTCGAAAAGCCATGGACTACTTGTCTGGTAAGAAAGAGGAGACCAAGCAGAAGGCTGCGGAGACTGCAGAGCAAACAAAAGATAAGGCTGCCGACACTACTGAGAAAACAAAAGAGAAAACTTACGAGACTGCAGAGAAAACCAAGGAGAAGTTGAGCGAGACTGAGGAGGAAGCAAGGAGAAAGATGGAGGAATTGAAGGTGGAAGGGAAAGAGTACAAGGACGAAGCTGGTGGTAGATCCCGTGAGGCACGTGACATTGAGGCTGAGAAAGGGAAGGCAGCCAAGGGAAATATATTGAGCTCATTTGGCGGAGTGACCGGTGCCATTAAAAGCAAGTTAACTCAGCCAACCGATGTTGTGGAGAGGGAGGAGGTTGATGTGGCGGAGACCAGGCCAGGTGAGGTGGCGGGTAAGCTCTATTCGTCTGACCAGATGCATGGTCAAAATTTCAACGACGTGGGTCGTTTGGATGGAGGAAAAGCGCGCGTGGAGGTGCACATTGATGACGACGGAAAAGTGAGGGTGGAGAGCCCGGGTAAAATGTGA

Protein Analysis

492

Amino Acids

53.21

Weight (kDa)

5.67

Isoelectric Point (pI)

24.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012043)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36640 AT2G36640 AT3G53040
fragaria_vesca FvH4_6g17400
malus_domestica MD09G1267600.v1.1
prunus_persica Prupe.7G121500_v2.0.a1 Prupe.7G121500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0471651
rosa_laevigata RLG00000024148
rosa_multiflora Rmu_co8483845.1_g000001 Rmu_sc0005631.1_g000008
rosa_roxburghii Rroxscaffold_6G00409690
rosa_rugosa Rorug03G0118600
rosa_samantha Rh3AG169800 Rh3BG195500 Rh3CG186200 Rh3CG195500 Rh3DG179500
rosa_wichuraiana Rw0G008960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 329
AccB1I GGYRCC 3 cut(s) 296, 329, 1250
AccI GTMKAC 3 cut(s) 455, 674, 773
AccII CGCG 4 cut(s) 302, 335, 1414, 1416
AclWI GGATC 1 cut(s) 1161
AcsI RAATTY 1 cut(s) 1375
AcuI CTGAAG 2 cut(s) 218, 926
AcvI CACGTG 1 cut(s) 1181
AcyI GRCGYC 2 cut(s) 5, 297
AfaI GTAC 3 cut(s) 323, 331, 1145
AfiI CCNNNNNNNGG 1 cut(s) 45
AgeI ACCGGT 1 cut(s) 1247
AgsI TTSAA 3 cut(s) 272, 1126, 1381
AhdI GACNNNNNGTC 1 cut(s) 949
AjiI CACGTC 2 cut(s) 73, 1387
AjnI CCWGG 3 cut(s) 175, 1319, 1324
AleI CACNNNNGTG 1 cut(s) 884
Alw21I GWGCWC 2 cut(s) 1232, 1428
Alw44I GTGCAC 1 cut(s) 1424
AlwI GGATC 1 cut(s) 1161
Ama87I CYCGRG 1 cut(s) 1462
ApaLI GTGCAC 1 cut(s) 1424
ApoI RAATTY 1 cut(s) 1375
ArsI GACNNNNNNTTYG 2 cut(s) 72, 104
AsiGI ACCGGT 1 cut(s) 1247
Asp718I GGTACC 1 cut(s) 329
AspLEI GCGC 4 cut(s) 299, 926, 1414, 1416
AsuC2I CCSGG 3 cut(s) 211, 1463, 1464
AsuHPI GGTGA 4 cut(s) 193, 217, 680, 1340
AvaI CYCGRG 1 cut(s) 1462
BaeGI GKGCMC 1 cut(s) 1428
BaeI ACNNNNGTAYC 2 cut(s) 313, 346
BanI GGYRCC 3 cut(s) 296, 329, 1250
BanII GRGCYC 2 cut(s) 1232, 1463
BbrPI CACGTG 1 cut(s) 1181
BbsI GAAGAC 2 cut(s) 206, 651
Bbv12I GWGCWC 2 cut(s) 1232, 1428
BccI CCATC 2 cut(s) 1108, 1394
BceAI ACGGC 3 cut(s) 381, 414, 930
BciT130I CCWGG 3 cut(s) 177, 1321, 1326
BcnI CCSGG 3 cut(s) 211, 1463, 1464
BfaI CTAG 1 cut(s) 218
BfmI CTRYAG 2 cut(s) 993, 1059
BfoI RGCGCY 2 cut(s) 300, 927
BglI GCCNNNNNGGC 4 cut(s) 518, 629, 803, 827
Bme1390I CCNGG 6 cut(s) 177, 211, 1321, 1326, 1463, 1464
BmeRI GACNNNNNGTC 1 cut(s) 949
BmeT110I CYCGRG 1 cut(s) 1462
BmgBI CACGTC 2 cut(s) 73, 1387
BmiI GGNNCC 3 cut(s) 298, 331, 1252
BmrFI CCNGG 6 cut(s) 177, 211, 1321, 1326, 1463, 1464
BmsI GCATC 3 cut(s) 223, 727, 1353
BoxI GACNNNNGTC 1 cut(s) 1389
BpiI GAAGAC 2 cut(s) 206, 651
Bpu10I CCTNAGC 1 cut(s) 612
BpuEI CTTGAG 1 cut(s) 489
BpuMI CCSGG 3 cut(s) 211, 1463, 1464
BsaAI YACGTR 1 cut(s) 1181
BsaHI GRCGYC 2 cut(s) 5, 297
BsaI GGTCTC 5 cut(s) 281, 578, 830, 962, 1310
BsaWI WCCGGW 1 cut(s) 1247
Bsc4I CCNNNNNNNGG 1 cut(s) 45
Bse118I RCCGGY 2 cut(s) 920, 1247
Bse1I ACTGG 1 cut(s) 463
Bse3DI GCAATG 1 cut(s) 660
BseBI CCWGG 3 cut(s) 177, 1321, 1326
BseGI GGATG 4 cut(s) 64, 238, 742, 1405
BseLI CCNNNNNNNGG 1 cut(s) 45
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 6 cut(s) 330, 681, 1020, 1083, 1185, 1286
BseNI ACTGG 1 cut(s) 463
BsePI GCGCGC 1 cut(s) 1412
BseRI GAGGAG 3 cut(s) 980, 1109, 1313
BseSI GKGCMC 1 cut(s) 1428
BseYI CCCAGC 1 cut(s) 139
Bsh1236I CGCG 4 cut(s) 302, 335, 1414, 1416
BshNI GGYRCC 3 cut(s) 296, 329, 1250
BshTI ACCGGT 1 cut(s) 1247
BsiHKAI GWGCWC 2 cut(s) 1232, 1428
BsiHKCI CYCGRG 1 cut(s) 1462
BsiSI CCGG 4 cut(s) 210, 921, 1248, 1463
BslI CCNNNNNNNGG 1 cut(s) 45
BsmBI CGTCTC 2 cut(s) 63, 632
Bso31I GGTCTC 5 cut(s) 281, 578, 830, 962, 1310
BsoBI CYCGRG 1 cut(s) 1462
Bsp1286I GDGCHC 3 cut(s) 1232, 1428, 1463
Bsp143I GATC 2 cut(s) 183, 1166
Bsp19I CCATGG 1 cut(s) 939
BspCNI CTCAG 6 cut(s) 331, 682, 1021, 1084, 1186, 1285
BspFNI CGCG 4 cut(s) 302, 335, 1414, 1416
BspLI GGNNCC 3 cut(s) 298, 331, 1252
BspMAI CTGCAG 2 cut(s) 997, 1063
BspPI GGATC 1 cut(s) 1161
BspT107I GGYRCC 3 cut(s) 296, 329, 1250
BspTNI GGTCTC 5 cut(s) 281, 578, 830, 962, 1310
BsrDI GCAATG 1 cut(s) 660
BsrFI RCCGGY 2 cut(s) 920, 1247
BsrI ACTGG 1 cut(s) 463
BssAI RCCGGY 2 cut(s) 920, 1247
BssHII GCGCGC 1 cut(s) 1412
BssMI GATC 2 cut(s) 183, 1166
BssNAI GTATAC 3 cut(s) 456, 675, 774
BssNI GRCGYC 2 cut(s) 5, 297
Bst1107I GTATAC 3 cut(s) 456, 675, 774
Bst2UI CCWGG 3 cut(s) 177, 1321, 1326
Bst4CI ACNGT 4 cut(s) 205, 354, 754, 886
Bst6I CTCTTC 3 cut(s) 20, 162, 267
BstACI GRCGYC 2 cut(s) 5, 297
BstAPI GCANNNNNTGC 1 cut(s) 737
BstBAI YACGTR 1 cut(s) 1181
BstC8I GCNNGC 8 cut(s) 15, 51, 150, 225, 766, 902, 922, 1414
BstDEI CTNAG 8 cut(s) 339, 489, 612, 690, 1029, 1092, 1194, 1272
BstDSI CCRYGG 4 cut(s) 291, 300, 333, 939
BstEII GGTNACC 1 cut(s) 205
BstF5I GGATG 4 cut(s) 64, 238, 742, 1405
BstFNI CGCG 4 cut(s) 302, 335, 1414, 1416
BstH2I RGCGCY 2 cut(s) 300, 927
BstHHI GCGC 4 cut(s) 299, 926, 1414, 1416
BstKTI GATC 2 cut(s) 186, 1169
BstMBI GATC 2 cut(s) 183, 1166
BstNI CCWGG 3 cut(s) 177, 1321, 1326
BstPAI GACNNNNGTC 1 cut(s) 1389
BstPI GGTNACC 1 cut(s) 205
BstSCI CCNGG 6 cut(s) 175, 209, 1319, 1324, 1461, 1462
BstSFI CTRYAG 2 cut(s) 993, 1059
BstSLI GKGCMC 1 cut(s) 1428
BstUI CGCG 4 cut(s) 302, 335, 1414, 1416
BstV2I GAAGAC 2 cut(s) 206, 651
BstX2I RGATCY 1 cut(s) 1166
BstXI CCANNNNNNTGG 1 cut(s) 1367
BstYI RGATCY 1 cut(s) 1166
BstZ17I GTATAC 3 cut(s) 456, 675, 774
BtgI CCRYGG 4 cut(s) 291, 300, 333, 939
BtrI CACGTC 2 cut(s) 73, 1387
BtsCI GGATG 4 cut(s) 64, 238, 742, 1405
BtsIMutI CAGTG 1 cut(s) 687
Cac8I GCNNGC 8 cut(s) 15, 51, 150, 225, 766, 902, 922, 1414
CfoI GCGC 4 cut(s) 299, 926, 1414, 1416
Cfr10I RCCGGY 2 cut(s) 920, 1247
Cfr42I CCGCGG 2 cut(s) 303, 336
Cfr9I CCCGGG 1 cut(s) 1462
CseI GACGC 4 cut(s) 395, 428, 503, 722
Csp6I GTAC 3 cut(s) 322, 330, 1144
CspAI ACCGGT 1 cut(s) 1247
CviAII CATG 4 cut(s) 164, 266, 940, 1367
CviQI GTAC 3 cut(s) 322, 330, 1144
DdeI CTNAG 8 cut(s) 339, 489, 612, 690, 1029, 1092, 1194, 1272
DinI GGCGCC 1 cut(s) 298
DpnI GATC 2 cut(s) 185, 1168
DpnII GATC 2 cut(s) 183, 1166
DriI GACNNNNNGTC 1 cut(s) 949
Eam1104I CTCTTC 3 cut(s) 20, 162, 267
Eam1105I GACNNNNNGTC 1 cut(s) 949
EarI CTCTTC 3 cut(s) 20, 162, 267
EciI GGCGGA 2 cut(s) 1254, 1328
Ecl136II GAGCTC 1 cut(s) 1230
Eco24I GRGCYC 2 cut(s) 1232, 1463
Eco31I GGTCTC 5 cut(s) 281, 578, 830, 962, 1310
Eco53kI GAGCTC 1 cut(s) 1230
Eco57I CTGAAG 2 cut(s) 218, 926
Eco72I CACGTG 1 cut(s) 1181
Eco88I CYCGRG 1 cut(s) 1462
Eco91I GGTNACC 1 cut(s) 205
EcoICRI GAGCTC 1 cut(s) 1230
EcoO65I GGTNACC 1 cut(s) 205
EcoRII CCWGG 3 cut(s) 175, 1319, 1324
EcoT22I ATGCAT 1 cut(s) 1368
EcoT38I GRGCYC 2 cut(s) 1232, 1463
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
Esp3I CGTCTC 2 cut(s) 63, 632
FaeI CATG 4 cut(s) 167, 269, 943, 1370
FatI CATG 4 cut(s) 163, 265, 939, 1366
FauI CCCGC 2 cut(s) 295, 1330
FblI GTMKAC 3 cut(s) 455, 674, 773
FokI GGATG 4 cut(s) 71, 245, 749, 1412
FriOI GRGCYC 2 cut(s) 1232, 1463
FspBI CTAG 1 cut(s) 218
GlaI GCGC 4 cut(s) 298, 925, 1413, 1415
GsaI CCCAGC 1 cut(s) 143
HaeII RGCGCY 2 cut(s) 300, 927
HapII CCGG 4 cut(s) 210, 921, 1248, 1463
HgaI GACGC 4 cut(s) 395, 428, 503, 722
HhaI GCGC 4 cut(s) 299, 926, 1414, 1416
Hin1I GRCGYC 2 cut(s) 5, 297
Hin1II CATG 4 cut(s) 167, 269, 943, 1370
Hin6I GCGC 4 cut(s) 297, 924, 1412, 1414
HinP1I GCGC 4 cut(s) 297, 924, 1412, 1414
HincII GTYRAC 1 cut(s) 1269
HindII GTYRAC 1 cut(s) 1269
HinfI GANTC 1 cut(s) 647
HpaI GTTAAC 1 cut(s) 1269
HpaII CCGG 4 cut(s) 210, 921, 1248, 1463
HphI GGTGA 4 cut(s) 193, 217, 680, 1340
Hpy166II GTNNAC 7 cut(s) 76, 324, 456, 675, 774, 1269, 1426
Hpy188I TCNGA 1 cut(s) 1357
Hpy188III TCNNGA 1 cut(s) 506
Hpy8I GTNNAC 7 cut(s) 76, 324, 456, 675, 774, 1269, 1426
Hpy99I CGWCG 3 cut(s) 10, 1388, 1442
HpyCH4III ACNGT 4 cut(s) 205, 354, 754, 886
HpyCH4IV ACGT 3 cut(s) 72, 1180, 1386
HpyCH4V TGCA 7 cut(s) 163, 467, 724, 995, 1061, 1366, 1426
HpyF3I CTNAG 8 cut(s) 339, 489, 612, 690, 1029, 1092, 1194, 1272
HpySE526I ACGT 3 cut(s) 72, 1180, 1386
Hsp92I GRCGYC 2 cut(s) 5, 297
Hsp92II CATG 4 cut(s) 167, 269, 943, 1370
HspAI GCGC 4 cut(s) 297, 924, 1412, 1414
KasI GGCGCC 1 cut(s) 296
KpnI GGTACC 1 cut(s) 333
KroI GCCGGC 1 cut(s) 920
KroNI GCCGGC 1 cut(s) 922
KspAI GTTAAC 1 cut(s) 1269
KspI CCGCGG 2 cut(s) 303, 336
Kzo9I GATC 2 cut(s) 183, 1166
LweI GCATC 3 cut(s) 223, 727, 1353
MaeI CTAG 1 cut(s) 218
MaeII ACGT 3 cut(s) 72, 1180, 1386
MaeIII GTNAC 3 cut(s) 205, 1181, 1243
MalI GATC 2 cut(s) 185, 1168
MboI GATC 2 cut(s) 183, 1166
MboII GAAGA 7 cut(s) 37, 107, 179, 211, 284, 322, 656
MflI RGATCY 1 cut(s) 1166
MhlI GDGCHC 3 cut(s) 1232, 1428, 1463
MluCI AATT 3 cut(s) 778, 1121, 1375
Mly113I GGCGCC 1 cut(s) 297
MlyI GAGTC 1 cut(s) 641
Mph1103I ATGCAT 1 cut(s) 1368
MroNI GCCGGC 1 cut(s) 920
MseI TTAA 2 cut(s) 1257, 1268
MslI CAYNNNNRTG 4 cut(s) 434, 533, 884, 1431
MspA1I CMGCKG 3 cut(s) 56, 302, 335
MspI CCGG 4 cut(s) 210, 921, 1248, 1463
MspR9I CCNGG 6 cut(s) 177, 211, 1321, 1326, 1463, 1464
MvaI CCWGG 3 cut(s) 177, 1321, 1326
MvnI CGCG 4 cut(s) 302, 335, 1414, 1416
NaeI GCCGGC 1 cut(s) 922
NarI GGCGCC 1 cut(s) 297
NciI CCSGG 3 cut(s) 211, 1463, 1464
NcoI CCATGG 1 cut(s) 939
NdeII GATC 2 cut(s) 183, 1166
NgoMIV GCCGGC 1 cut(s) 920
NlaIII CATG 4 cut(s) 167, 269, 943, 1370
NlaIV GGNNCC 3 cut(s) 298, 331, 1252
NmeAIII GCCGAG 4 cut(s) 58, 629, 649, 847
NmuCI GTSAC 3 cut(s) 205, 1181, 1243
NsiI ATGCAT 1 cut(s) 1368
OliI CACNNNNGTG 1 cut(s) 884
PauI GCGCGC 1 cut(s) 1412
PdiI GCCGGC 1 cut(s) 922
PinAI ACCGGT 1 cut(s) 1247
PleI GAGTC 1 cut(s) 641
PluTI GGCGCC 1 cut(s) 300
PmaCI CACGTG 1 cut(s) 1181
PmlI CACGTG 1 cut(s) 1181
PpsI GAGTC 1 cut(s) 641
Ppu21I YACGTR 1 cut(s) 1181
PshAI GACNNNNGTC 1 cut(s) 1389
Psp124BI GAGCTC 1 cut(s) 1232
Psp6I CCWGG 3 cut(s) 175, 1319, 1324
PspCI CACGTG 1 cut(s) 1181
PspEI GGTNACC 1 cut(s) 205
PspFI CCCAGC 1 cut(s) 139
PspGI CCWGG 3 cut(s) 175, 1319, 1324
PspN4I GGNNCC 3 cut(s) 298, 331, 1252
PstI CTGCAG 2 cut(s) 997, 1063
PsuI RGATCY 1 cut(s) 1166
PteI GCGCGC 1 cut(s) 1412
RsaI GTAC 3 cut(s) 323, 331, 1145
RsaNI GTAC 3 cut(s) 322, 330, 1144
RseI CAYNNNNRTG 4 cut(s) 434, 533, 884, 1431
SacI GAGCTC 1 cut(s) 1232
SacII CCGCGG 2 cut(s) 303, 336
SaqAI TTAA 2 cut(s) 1257, 1268
Sau3AI GATC 2 cut(s) 183, 1166
SchI GAGTC 1 cut(s) 641
ScrFI CCNGG 6 cut(s) 177, 211, 1321, 1326, 1463, 1464
SduI GDGCHC 3 cut(s) 1232, 1428, 1463
SfaNI GCATC 3 cut(s) 223, 727, 1353
SfcI CTRYAG 2 cut(s) 993, 1059
SfiI GGCCNNNNNGGCC 1 cut(s) 803
SfoI GGCGCC 1 cut(s) 298
Sfr303I CCGCGG 2 cut(s) 303, 336
SgrBI CCGCGG 2 cut(s) 303, 336
SmaI CCCGGG 1 cut(s) 1464
SmiMI CAYNNNNRTG 4 cut(s) 434, 533, 884, 1431
SmlI CTYRAG 1 cut(s) 504
SmoI CTYRAG 1 cut(s) 504
Sse9I AATT 3 cut(s) 778, 1121, 1375
SspDI GGCGCC 1 cut(s) 296
SspMI CTAG 1 cut(s) 218
SstI GAGCTC 1 cut(s) 1232
StyD4I CCNGG 6 cut(s) 175, 209, 1319, 1324, 1461, 1462
TaaI ACNGT 4 cut(s) 205, 354, 754, 886
TaiI ACGT 3 cut(s) 75, 1183, 1389
TaqI TCGA 2 cut(s) 8, 931
TasI AATT 3 cut(s) 778, 1121, 1375
TatI WGTACW 2 cut(s) 321, 1143
TauI GCSGC 4 cut(s) 41, 302, 338, 623
Tru1I TTAA 2 cut(s) 1257, 1268
Tru9I TTAA 2 cut(s) 1257, 1268
TscAI CASTG 1 cut(s) 694
TseFI GTSAC 3 cut(s) 205, 1181, 1243
Tsp45I GTSAC 3 cut(s) 205, 1181, 1243
TspDTI ATGAA 3 cut(s) 108, 180, 273
TspGWI ACGGA 2 cut(s) 656, 1455
TspMI CCCGGG 1 cut(s) 1462
TspRI CASTG 1 cut(s) 694
VneI GTGCAC 1 cut(s) 1424
XapI RAATTY 1 cut(s) 1375
XmaI CCCGGG 1 cut(s) 1462
XmiI GTMKAC 3 cut(s) 455, 674, 773
XspI CTAG 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.