Rh3BG195500

Embryonic protein DC-8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0012043
Physical Location & Seq
Forward (+)
17297931 .. 17300063
2133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG195500.1

Sequence Viewer

Length: 1236 bp
ATGGCGTCGTCGAAGCAGTTCAAGGAGGAGAGAGCTGAGGCAGAAGCGAGGAAAGCCGCGCTTGATCTGAAAGATGGGAACAGAGAAAGAGAGTACGAAACAGCTGGGGTGAAGATGGACAGGGACATGTACGGCCAGCCAAGAGAGCCATCCCATGTGCAACAAGAAGCGAAGCCTGGTGTTATTGGGTCTGTTTTTAGGGCTGTGACCGGCACATTGGAGCATGCAAAGGACGCTGTTGTGGGCAAAAGCCATGACACTTCTGATGCAGCAAGAGAGCGTTCGGAATACACCACACTTAAGGCGAAGGAAACGACTGATGCGGCTGGAAAGAAGCTGGAGGAGTATAAGGAGGCCGCGGCTCGGCCGGCGAAGGAGGCTGGGGAGAAGACGAAGGAGGTGAAGGACACTGCTGCGGAGAAGGCCAAAGAGACCAAGGAGTCTGCCAAGGGGAAGGGGAGTGAGTATGCGGATTACACTTCGCAGAAGGCGAAGGAGGCCAAGGATGCCGCGGCGGAGAAGGCTAAGCAGACGAAAGACTATGTTGCCGAGACCGCAAGTGAGGCTACGGAGAAGGCGAAAGAGACAAAGGATGCTGCGATGAGGAAGGCTAAGGAGTACACGGATTACACTGCTCAGAAGGCCAAGGAAGCCAAGGATACCACAGTAGGGAAGGCTAAAGGGTACACCAATTATGGGGCCGAGAAGGCAGAGGAGGCCAAGGACACTACAGTGGGGAAGGCCGGTGAGTACACGAACTATGCTGCCGAGAAGGCAAAGGGGGCCAAGGACACTACGGTGGGGAAGGCGGAGGAGTACAAGAATTACACCGCAGAAAAGGCAAAGGAGACTGCGGAGGCAACCAAAGTAAAGGCAAAAGAGACTGCGGAGGCAGCCAAAGACAAGACTAAAGAGACAGCGGAGTATACAGCTGAGAAAGCAAAAGAAGGGAAGGACACTACTGTGAGCAAGTTGGGTGGGCTCATGAATTACTTGACTGGTAAGAAAGAAGAAACCAAACAGAAGGCTGCAGAGACGGCTGGGACTGTCAAAGAGAACACTAAACAGGTGGCTGAGGCGGCGAGGGAGAAGACTGAAGAGACTGCCGAGGCAACCAAACAGAAGACCAAAGAGGCAACTGAAAAAACCAAGGTGATAATGAGTTATCAAAGTCGCTTAATTATGAGACGTTGGGTGAGACCAAGGAGGAAGCTAGGAGAAAGATGGAGGAGCTGA

Protein Analysis

411

Amino Acids

45.26

Weight (kDa)

9.19

Isoelectric Point (pI)

23.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_4 PF02987 157 - 189 2.5e-07 Late embryogenesis abundant protein
LEA_4 PF02987 189 - 229 2e-13 Late embryogenesis abundant protein
LEA_4 PF02987 230 - 273 3e-16 Late embryogenesis abundant protein
LEA_4 PF02987 252 - 295 3e-10 Late embryogenesis abundant protein
LEA_4 PF02987 288 - 326 4.5e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012043)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36640 AT2G36640 AT3G53040
fragaria_vesca FvH4_6g17400
malus_domestica MD09G1267600.v1.1
prunus_persica Prupe.7G121500_v2.0.a1 Prupe.7G121500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0471651
rosa_laevigata RLG00000024148
rosa_multiflora Rmu_co8483845.1_g000001 Rmu_sc0005631.1_g000008
rosa_roxburghii Rroxscaffold_6G00409690
rosa_rugosa Rorug03G0118600
rosa_samantha Rh3AG169800 Rh3BG195500 Rh3CG186200 Rh3CG195500 Rh3DG179500
rosa_wichuraiana Rw0G008960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 439
AccI GTMKAC 1 cut(s) 926
AccII CGCG 3 cut(s) 59, 359, 512
AcoI YGGCCR 2 cut(s) 133, 365
AcuI CTGAAG 1 cut(s) 1116
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 6 cut(s) 95, 131, 620, 686, 752, 818
AfiI CCNNNNNNNGG 3 cut(s) 363, 669, 696
AflII CTTAAG 1 cut(s) 299
AflIII ACRYGT 1 cut(s) 126
AgsI TTSAA 1 cut(s) 22
AjnI CCWGG 1 cut(s) 175
AleI CACNNNNGTG 3 cut(s) 731, 797, 962
AluBI AGCT 6 cut(s) 35, 104, 337, 932, 1213, 1233
AluI AGCT 6 cut(s) 35, 104, 337, 932, 1213, 1233
ApeKI GCWGC 6 cut(s) 269, 413, 596, 764, 893, 1028
Asp700I GAANNNNTTC 1 cut(s) 17
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 2 cut(s) 699, 783
AsuHPI GGTGA 5 cut(s) 121, 412, 758, 1165, 1207
BaeI ACNNNNGTAYC 2 cut(s) 651, 684
BanII GRGCYC 1 cut(s) 984
BbsI GAAGAC 3 cut(s) 395, 1097, 1130
BbvCI CCTCAGC 2 cut(s) 36, 1074
BbvI GCAGC 6 cut(s) 281, 400, 583, 751, 905, 1015
BccI CCATC 4 cut(s) 68, 109, 157, 1218
BceAI ACGGC 2 cut(s) 148, 1053
BciT130I CCWGG 1 cut(s) 177
BciVI GTATCC 1 cut(s) 652
BfaI CTAG 1 cut(s) 1214
BfmI CTRYAG 2 cut(s) 729, 1029
BfrI CTTAAG 1 cut(s) 299
BfuI GTATCC 1 cut(s) 652
BglI GCCNNNNNGGC 2 cut(s) 707, 773
BlpI GCTNAGC 1 cut(s) 525
Bme1390I CCNGG 1 cut(s) 177
BmgT120I GGNCC 2 cut(s) 699, 783
BmiI GGNNCC 2 cut(s) 700, 784
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 4 cut(s) 256, 310, 496, 583
BpiI GAAGAC 3 cut(s) 395, 1097, 1130
BpmI CTGGAG 1 cut(s) 359
Bpu10I CCTNAGC 3 cut(s) 36, 612, 1074
Bpu1102I GCTNAGC 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 3 cut(s) 425, 545, 1192
Bsc4I CCNNNNNNNGG 3 cut(s) 363, 669, 696
Bse118I RCCGGY 3 cut(s) 209, 367, 743
Bse1I ACTGG 1 cut(s) 1003
BseBI CCWGG 1 cut(s) 177
BseGI GGATG 3 cut(s) 149, 511, 598
BseLI CCNNNNNNNGG 3 cut(s) 363, 669, 696
BseMII CTCAG 4 cut(s) 27, 650, 924, 1065
BseNI ACTGG 1 cut(s) 1003
BseRI GAGGAG 4 cut(s) 41, 356, 728, 827
BseX3I CGGCCG 1 cut(s) 365
BseXI GCAGC 6 cut(s) 281, 400, 583, 751, 905, 1015
BseYI CCCAGC 3 cut(s) 104, 380, 1040
Bsh1236I CGCG 3 cut(s) 59, 359, 512
Bsh1285I CGRYCG 1 cut(s) 368
BsiEI CGRYCG 1 cut(s) 368
BsiSI CCGG 3 cut(s) 210, 368, 744
BslFI GGGAC 2 cut(s) 137, 1057
BslI CCNNNNNNNGG 3 cut(s) 363, 669, 696
BsmBI CGTCTC 2 cut(s) 1028, 1180
BsmFI GGGAC 2 cut(s) 137, 1057
Bso31I GGTCTC 3 cut(s) 425, 545, 1192
Bsp1286I GDGCHC 1 cut(s) 984
Bsp143I GATC 1 cut(s) 64
Bsp1720I GCTNAGC 1 cut(s) 525
BspCNI CTCAG 4 cut(s) 28, 649, 925, 1066
BspFNI CGCG 3 cut(s) 59, 359, 512
BspHI TCATGA 1 cut(s) 984
BspLI GGNNCC 2 cut(s) 700, 784
BspMAI CTGCAG 1 cut(s) 1033
BspTI CTTAAG 1 cut(s) 299
BspTNI GGTCTC 3 cut(s) 425, 545, 1192
BsrFI RCCGGY 3 cut(s) 209, 367, 743
BsrI ACTGG 1 cut(s) 1003
BssAI RCCGGY 3 cut(s) 209, 367, 743
BssMI GATC 1 cut(s) 64
BssNAI GTATAC 1 cut(s) 927
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 9 cut(s) 435, 447, 501, 645, 654, 720, 786, 1149, 1202
Bst1107I GTATAC 1 cut(s) 927
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 5 cut(s) 667, 733, 799, 964, 1048
Bst6I CTCTTC 1 cut(s) 1092
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 299
BstC8I GCNNGC 3 cut(s) 137, 225, 369
BstDEI CTNAG 6 cut(s) 36, 525, 612, 636, 933, 1074
BstDSI CCRYGG 2 cut(s) 357, 510
BstF5I GGATG 3 cut(s) 149, 511, 598
BstFNI CGCG 3 cut(s) 59, 359, 512
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 1 cut(s) 67
BstMBI GATC 1 cut(s) 64
BstMCI CGRYCG 1 cut(s) 368
BstNI CCWGG 1 cut(s) 177
BstNSI RCATGY 2 cut(s) 130, 227
BstSCI CCNGG 1 cut(s) 175
BstSFI CTRYAG 2 cut(s) 729, 1029
BstUI CGCG 3 cut(s) 59, 359, 512
BstV1I GCAGC 6 cut(s) 281, 400, 583, 751, 905, 1015
BstV2I GAAGAC 3 cut(s) 395, 1097, 1130
BstZ17I GTATAC 1 cut(s) 927
BstZI CGGCCG 1 cut(s) 365
BsuI GTATCC 1 cut(s) 652
BtgI CCRYGG 2 cut(s) 357, 510
BtgZI GCGATG 1 cut(s) 614
BtsCI GGATG 3 cut(s) 149, 511, 598
BtsI GCAGTG 2 cut(s) 408, 630
BtsIMutI CAGTG 3 cut(s) 408, 630, 738
Cac8I GCNNGC 3 cut(s) 137, 225, 369
CciI TCATGA 1 cut(s) 984
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 3 cut(s) 209, 367, 743
Cfr13I GGNCC 2 cut(s) 699, 783
Cfr42I CCGCGG 2 cut(s) 360, 513
CseI GACGC 1 cut(s) 242
Csp6I GTAC 6 cut(s) 94, 130, 619, 685, 751, 817
CspCI CAANNNNNGTGG 2 cut(s) 958, 993
CviAII CATG 5 cut(s) 127, 155, 224, 254, 985
CviQI GTAC 6 cut(s) 94, 130, 619, 685, 751, 817
DdeI CTNAG 6 cut(s) 36, 525, 612, 636, 933, 1074
DpnI GATC 1 cut(s) 66
DpnII GATC 1 cut(s) 64
DrdI GACNNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 439
EaeI YGGCCR 2 cut(s) 133, 365
EagI CGGCCG 1 cut(s) 365
Eam1104I CTCTTC 1 cut(s) 1092
EarI CTCTTC 1 cut(s) 1092
EciI GGCGGA 2 cut(s) 530, 824
EclXI CGGCCG 1 cut(s) 365
Eco130I CCWWGG 9 cut(s) 435, 447, 501, 645, 654, 720, 786, 1149, 1202
Eco24I GRGCYC 1 cut(s) 984
Eco31I GGTCTC 3 cut(s) 425, 545, 1192
Eco52I CGGCCG 1 cut(s) 365
Eco57I CTGAAG 1 cut(s) 1116
EcoRII CCWGG 1 cut(s) 175
EcoT14I CCWWGG 9 cut(s) 435, 447, 501, 645, 654, 720, 786, 1149, 1202
EcoT38I GRGCYC 1 cut(s) 984
ErhI CCWWGG 9 cut(s) 435, 447, 501, 645, 654, 720, 786, 1149, 1202
Esp3I CGTCTC 2 cut(s) 1028, 1180
FaeI CATG 5 cut(s) 130, 158, 227, 257, 988
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 2 cut(s) 137, 1057
FatI CATG 5 cut(s) 126, 154, 223, 253, 984
FblI GTMKAC 1 cut(s) 926
FokI GGATG 3 cut(s) 136, 518, 605
FriOI GRGCYC 1 cut(s) 984
FspBI CTAG 1 cut(s) 1214
GlaI GCGC 1 cut(s) 60
GsaI CCCAGC 3 cut(s) 108, 384, 1044
GsuI CTGGAG 1 cut(s) 359
HapII CCGG 3 cut(s) 210, 368, 744
HgaI GACGC 1 cut(s) 242
HhaI GCGC 1 cut(s) 61
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 130, 158, 227, 257, 988
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HinfI GANTC 1 cut(s) 440
HpaII CCGG 3 cut(s) 210, 368, 744
HphI GGTGA 5 cut(s) 121, 412, 758, 1165, 1207
Hpy166II GTNNAC 4 cut(s) 621, 687, 753, 927
Hpy188I TCNGA 4 cut(s) 69, 265, 286, 639
Hpy188III TCNNGA 1 cut(s) 985
Hpy8I GTNNAC 4 cut(s) 621, 687, 753, 927
Hpy99I CGWCG 2 cut(s) 10, 13
HpyCH4III ACNGT 5 cut(s) 667, 733, 799, 964, 1048
HpyCH4IV ACGT 1 cut(s) 1189
HpyCH4V TGCA 4 cut(s) 160, 227, 269, 1031
HpyF3I CTNAG 6 cut(s) 36, 525, 612, 636, 933, 1074
HpySE526I ACGT 1 cut(s) 1189
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 130, 158, 227, 257, 988
HspAI GCGC 1 cut(s) 59
KroI GCCGGC 1 cut(s) 367
KroNI GCCGGC 1 cut(s) 369
KspI CCGCGG 2 cut(s) 360, 513
Kzo9I GATC 1 cut(s) 64
LmnI GCTCC 2 cut(s) 220, 1230
Lsp1109I GCAGC 6 cut(s) 281, 400, 583, 751, 905, 1015
LweI GCATC 4 cut(s) 256, 310, 496, 583
MaeI CTAG 1 cut(s) 1214
MaeII ACGT 1 cut(s) 1189
MaeIII GTNAC 1 cut(s) 205
MalI GATC 1 cut(s) 66
MboI GATC 1 cut(s) 64
MboII GAAGA 6 cut(s) 124, 400, 1022, 1102, 1109, 1135
MhlI GDGCHC 1 cut(s) 984
MluCI AATT 4 cut(s) 691, 823, 988, 1179
MlyI GAGTC 1 cut(s) 449
MroNI GCCGGC 1 cut(s) 367
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 2 cut(s) 300, 1178
MslI CAYNNNNRTG 3 cut(s) 731, 797, 962
MspA1I CMGCKG 5 cut(s) 104, 359, 512, 920, 932
MspCI CTTAAG 1 cut(s) 299
MspI CCGG 3 cut(s) 210, 368, 744
MspR9I CCNGG 1 cut(s) 177
MvaI CCWGG 1 cut(s) 177
MvnI CGCG 3 cut(s) 59, 359, 512
NaeI GCCGGC 1 cut(s) 369
NdeII GATC 1 cut(s) 64
NgoMIV GCCGGC 1 cut(s) 367
NlaIII CATG 5 cut(s) 130, 158, 227, 257, 988
NlaIV GGNNCC 2 cut(s) 700, 784
NmeAIII GCCGAG 5 cut(s) 343, 574, 727, 793, 1132
NmuCI GTSAC 1 cut(s) 205
NspI RCATGY 2 cut(s) 130, 227
OliI CACNNNNGTG 3 cut(s) 731, 797, 962
PaeI GCATGC 1 cut(s) 227
PagI TCATGA 1 cut(s) 984
PciI ACATGT 1 cut(s) 126
PcsI WCGNNNNNNNCGW 2 cut(s) 488, 575
PdiI GCCGGC 1 cut(s) 369
PdmI GAANNNNTTC 1 cut(s) 17
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PscI ACATGT 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 175
PspFI CCCAGC 3 cut(s) 104, 380, 1040
PspGI CCWGG 1 cut(s) 175
PspN4I GGNNCC 2 cut(s) 700, 784
PspPI GGNCC 2 cut(s) 699, 783
PstI CTGCAG 1 cut(s) 1033
PvuII CAGCTG 2 cut(s) 104, 932
RsaI GTAC 6 cut(s) 95, 131, 620, 686, 752, 818
RsaNI GTAC 6 cut(s) 94, 130, 619, 685, 751, 817
RseI CAYNNNNRTG 3 cut(s) 731, 797, 962
SacII CCGCGG 2 cut(s) 360, 513
SaqAI TTAA 2 cut(s) 300, 1178
Sau3AI GATC 1 cut(s) 64
Sau96I GGNCC 2 cut(s) 699, 783
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 1 cut(s) 177
SduI GDGCHC 1 cut(s) 984
SfaNI GCATC 4 cut(s) 256, 310, 496, 583
SfcI CTRYAG 2 cut(s) 729, 1029
Sfr303I CCGCGG 2 cut(s) 360, 513
SgrBI CCGCGG 2 cut(s) 360, 513
SmiMI CAYNNNNRTG 3 cut(s) 731, 797, 962
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
SphI GCATGC 1 cut(s) 227
Sse9I AATT 4 cut(s) 691, 823, 988, 1179
SspMI CTAG 1 cut(s) 1214
StyD4I CCNGG 1 cut(s) 175
StyI CCWWGG 9 cut(s) 435, 447, 501, 645, 654, 720, 786, 1149, 1202
TaaI ACNGT 5 cut(s) 667, 733, 799, 964, 1048
TaiI ACGT 1 cut(s) 1192
TaqI TCGA 1 cut(s) 11
TasI AATT 4 cut(s) 691, 823, 988, 1179
TatI WGTACW 3 cut(s) 618, 750, 816
TauI GCSGC 7 cut(s) 59, 326, 359, 362, 512, 515, 1082
Tru1I TTAA 2 cut(s) 300, 1178
Tru9I TTAA 2 cut(s) 300, 1178
TscAI CASTG 3 cut(s) 415, 637, 738
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 6 cut(s) 269, 413, 596, 764, 893, 1028
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 1 cut(s) 1001
TspGWI ACGGA 2 cut(s) 584, 638
TspRI CASTG 3 cut(s) 415, 637, 738
Vha464I CTTAAG 1 cut(s) 299
XceI RCATGY 2 cut(s) 130, 227
XmiI GTMKAC 1 cut(s) 926
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 1 cut(s) 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.