Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0012043

Physical Location & Seq

Genomic Coordinates Reverse (-)

32476674 .. 32478411

Gene Structure 1738 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024148

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGGCGTCATCGAAGCAGTTCAAGGAGGAGAGAGCTGAGGCAGAAGCGAGGAAAGCCGCGCTTGATCTGAAAGATGAGAACAGAGAAAGAGAGTACGAAACAGCTGGGGTGAAGATGGACAGGGACATGTACGGCCAGCCAAGAGAGCTATCCCATGTGCAACAAGAAGCGAAGCCTGGTGTTATTGGGTCTGTTTTTAGGGCTGTGACCGGCACATTGGAGCATGCAAAGGACGCTGTTGTGGGCAAAAGCCATGACACTTCTGATGCAGCAAGAGAGCGTTCGGAATACACCACACTTAAGGCGAAGGAAACGACTGATGCGGCTGGAGAGAAGCTGGAGGAGTATAAGGAGGCCGCAGCTCGGCCGGCGAAGGAGGCTGCGGAGAAGACGCAGGAGGTGAAGGACACTGCTGCGGAGAAGGCCAGAGAGACCAAGGAGTCTGCCAAGGGGAAGGGGAGTGAGTATGCGGATTACACTTCGCAGAAGGCGAAAGAGGCCAAGGATGCCGCGGCGGAGAAGGCTAGGCAGACGAAAGACTATGTTGCCGAGACCGCAAGTGAGGCTACGGAGAAGGCGAAAGAGACAAAGGATGCTGCAATGAGGAAGGCTGAGGAGTACACTGATTACACTGCTCAGAAGGCCAAGGAGGCCAAGGATACGACAGTAGGGAAGGCTAAAGAGTACACCAATTATGCGGCCGACAAGGCAAAGGAGGCCAAGGACACTACAGTGGGGAAGGCCAGTGAGTACACGAACTATGCTGCCGAGAAGGCAAAGGGGGCCAAGGACACTACGGTGGGGAAAGCGGAGGAGTACAAGAATTACTCAGCAGAAAAGGCAAAGGAGACTGCGGAGGCAACCAAAGAAAAGGCAAAAGAGACTGCGGAGGCAGCCAAAGATAAGACTAAAGAGACAGCGGAGTATACAGCTGAGAAAGCAAAAGAAGGGAAGGACACTACTGTGAGCAAGTTGGGTGGGCTCATGAATTACTTGACTGGTAAGAAAGAAGAAACCAAACAGAAGGCTGCAGAGACGGCTGAGACTGTCAAAGAGAACACTAAACAGGCGGCTGAGGCGGCGAAGGAGAAGACTGAAGAGACTGCCGAGGCAACCGAACAGAAGACCAAAGAGGCAGCTGAGAAAACCAAGGAGACGTTGGGTGAGACCGAGGAGGAAGCTAGGAGAAAGATGGAGGAGCTGAGGCTTAGGAAGAAAGGTTATGATGCGAAAGCAGGAGACATGGACCGTGACGATGACCGTGAGATTGAGGCAGCTAAGGGCACTGCAGCAAAGAGCAACATTTTCAACTCATTTGGGAGTGTGACTGATGCTATCAAGAGCAAGCTGATTCATCCAACCGTTGTGGTGGAGGAGTCACGTGCTGCACGTGAGGAGGGGAGTACAAAAAGGACCAAGACTCCTGCTATGGTAGAGGTACCTGTGGAGAAGGTGCAGATCGACATTACGGACGCGCCCCCGGGGGCTGTGGCCGCGGCACTTAAGAAGTCTGACCAGATGTTTGGTCAAACTTTCAACGATCCTGGTAGAATGGGAGATGAGGGCAAGGTGGTTGTGGAGCGGCGCGTGGTGGACGAAGCCGGAAATGTGCGTGTGGACCGCAGTGGCAAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0007275 GO:0008150 GO:0009791 GO:0009838 GO:0009908 GO:0010227 GO:0022414 GO:0032501 GO:0032502 GO:0044424 GO:0044444 GO:0044464 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LEA_4

Protein Analysis

546

Amino Acids

59.66

Weight (kDa)

5.75

Isoelectric Point (pI)

26.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LEA_4	PF02987	157 - 189	1.8e-07	Late embryogenesis abundant protein
LEA_4	PF02987	189 - 229	2.6e-14	Late embryogenesis abundant protein
LEA_4	PF02987	230 - 273	8.5e-16	Late embryogenesis abundant protein
LEA_4	PF02987	252 - 294	5.6e-10	Late embryogenesis abundant protein
LEA_4	PF02987	287 - 326	5.1e-07	Late embryogenesis abundant protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012043)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36640 AT2G36640 AT3G53040
fragaria_vesca	FvH4_6g17400
malus_domestica	MD09G1267600.v1.1
prunus_persica	Prupe.7G121500_v2.0.a1 Prupe.7G121500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0471651
rosa_laevigata	RLG00000024148
rosa_multiflora	Rmu_co8483845.1_g000001 Rmu_sc0005631.1_g000008
rosa_roxburghii	Rroxscaffold_6G00409690
rosa_rugosa	Rorug03G0118600
rosa_samantha	Rh3AG169800 Rh3BG195500 Rh3CG186200 Rh3CG195500 Rh3DG179500
rosa_wichuraiana	Rw0G008960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	439
Acc65I	GGTACC	1 cut(s)	1438
AccB1I	GGYRCC	1 cut(s)	1438
AccBSI	CCGCTC	1 cut(s)	1582
AccI	GTMKAC	1 cut(s)	926
AccII	CGCG	5 cut(s)	59, 512, 1475, 1496, 1587
AclWI	GGATC	1 cut(s)	1535
AcoI	YGGCCR	4 cut(s)	133, 365, 699, 1491
AcuI	CTGAAG	1 cut(s)	1116
AcvI	CACGTG	2 cut(s)	1382, 1391
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	8 cut(s)	95, 131, 620, 686, 752, 818, 1405, 1440
AfiI	CCNNNNNNNGG	1 cut(s)	363
AflII	CTTAAG	2 cut(s)	299, 1502
AflIII	ACRYGT	1 cut(s)	126
AgsI	TTSAA	3 cut(s)	22, 1309, 1537
AjnI	CCWGG	2 cut(s)	175, 1543
AleI	CACNNNNGTG	3 cut(s)	731, 797, 962
AlwI	GGATC	1 cut(s)	1535
Ama87I	CYCGRG	1 cut(s)	1480
ArsI	GACNNNNNNTTYG	2 cut(s)	1505, 1537
Asp700I	GAANNNNTTC	1 cut(s)	17
Asp718I	GGTACC	1 cut(s)	1438
AspLEI	GCGC	3 cut(s)	61, 1477, 1587
AspS9I	GGNCC	4 cut(s)	783, 1246, 1413, 1618
AsuC2I	CCSGG	2 cut(s)	1481, 1482
AsuHPI	GGTGA	3 cut(s)	121, 412, 1175
AvaI	CYCGRG	1 cut(s)	1480
AvaII	GGWCC	3 cut(s)	1246, 1413, 1618
BaeGI	GKGCMC	1 cut(s)	1286
BaeI	ACNNNNGTAYC	2 cut(s)	651, 684
BanI	GGYRCC	1 cut(s)	1438
BanII	GRGCYC	1 cut(s)	984
BbrPI	CACGTG	2 cut(s)	1382, 1391
BbsI	GAAGAC	3 cut(s)	395, 1097, 1130
BbvCI	CCTCAGC	4 cut(s)	36, 612, 1074, 1202
BccI	CCATC	2 cut(s)	109, 1186
BceAI	ACGGC	2 cut(s)	148, 1053
BciT130I	CCWGG	2 cut(s)	177, 1545
BciVI	GTATCC	1 cut(s)	652
BcnI	CCSGG	2 cut(s)	1481, 1482
BfaI	CTAG	2 cut(s)	525, 1182
BfmI	CTRYAG	3 cut(s)	729, 1029, 1287
BfrI	CTTAAG	2 cut(s)	299, 1502
BfuI	GTATCC	1 cut(s)	652
BglI	GCCNNNNNGGC	3 cut(s)	650, 707, 773
Bme1390I	CCNGG	4 cut(s)	177, 1481, 1482, 1545
Bme18I	GGWCC	3 cut(s)	1246, 1413, 1618
BmeT110I	CYCGRG	1 cut(s)	1480
BmgT120I	GGNCC	4 cut(s)	783, 1246, 1413, 1618
BmiI	GGNNCC	2 cut(s)	784, 1440
BmrFI	CCNGG	4 cut(s)	177, 1481, 1482, 1545
BmsI	GCATC	6 cut(s)	256, 310, 496, 583, 1216, 1321
BpiI	GAAGAC	3 cut(s)	395, 1097, 1130
BpmI	CTGGAG	2 cut(s)	348, 359
Bpu10I	CCTNAGC	6 cut(s)	36, 612, 1074, 1202, 1208, 1278
BpuMI	CCSGG	2 cut(s)	1481, 1482
BsaAI	YACGTR	2 cut(s)	1382, 1391
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	3 cut(s)	425, 545, 1160
BsaXI	ACNNNNNCTCC	2 cut(s)	1405, 1435
Bsc4I	CCNNNNNNNGG	1 cut(s)	363
Bse118I	RCCGGY	2 cut(s)	209, 367
Bse1I	ACTGG	2 cut(s)	744, 1003
Bse3DI	GCAATG	1 cut(s)	606
BseBI	CCWGG	2 cut(s)	177, 1545
BseGI	GGATG	3 cut(s)	511, 598, 1354
BseLI	CCNNNNNNNGG	1 cut(s)	363
BseMI	GCAATG	1 cut(s)	606
BseMII	CTCAG	9 cut(s)	27, 603, 650, 843, 924, 1032, 1065, 1131, 1193
BseNI	ACTGG	2 cut(s)	744, 1003
BseRI	GAGGAG	8 cut(s)	41, 356, 629, 827, 1187, 1211, 1388, 1409
BseSI	GKGCMC	1 cut(s)	1286
BseX3I	CGGCCG	2 cut(s)	365, 699
BseYI	CCCAGC	1 cut(s)	104
BsgI	GTGCAG	2 cut(s)	1371, 1475
Bsh1236I	CGCG	5 cut(s)	59, 512, 1475, 1496, 1587
Bsh1285I	CGRYCG	2 cut(s)	368, 702
BshNI	GGYRCC	1 cut(s)	1438
BsiEI	CGRYCG	2 cut(s)	368, 702
BsiHKCI	CYCGRG	1 cut(s)	1480
BsiSI	CCGG	4 cut(s)	210, 368, 1481, 1602
BslFI	GGGAC	1 cut(s)	137
BslI	CCNNNNNNNGG	1 cut(s)	363
BsmBI	CGTCTC	2 cut(s)	1028, 1148
BsmFI	GGGAC	1 cut(s)	137
Bso31I	GGTCTC	3 cut(s)	425, 545, 1160
BsoBI	CYCGRG	1 cut(s)	1480
Bsp1286I	GDGCHC	2 cut(s)	984, 1286
Bsp143I	GATC	3 cut(s)	64, 1458, 1540
BspCNI	CTCAG	9 cut(s)	28, 604, 649, 842, 925, 1033, 1066, 1132, 1194
BspFNI	CGCG	5 cut(s)	59, 512, 1475, 1496, 1587
BspHI	TCATGA	1 cut(s)	984
BspLI	GGNNCC	2 cut(s)	784, 1440
BspMAI	CTGCAG	2 cut(s)	1033, 1291
BspPI	GGATC	1 cut(s)	1535
BspT107I	GGYRCC	1 cut(s)	1438
BspTI	CTTAAG	2 cut(s)	299, 1502
BspTNI	GGTCTC	3 cut(s)	425, 545, 1160
BsrBI	CCGCTC	1 cut(s)	1582
BsrDI	GCAATG	1 cut(s)	606
BsrFI	RCCGGY	2 cut(s)	209, 367
BsrI	ACTGG	2 cut(s)	744, 1003
BssAI	RCCGGY	2 cut(s)	209, 367
BssMI	GATC	3 cut(s)	64, 1458, 1540
BssNAI	GTATAC	1 cut(s)	927
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	8 cut(s)	435, 447, 501, 645, 654, 720, 786, 1149
Bst1107I	GTATAC	1 cut(s)	927
Bst2UI	CCWGG	2 cut(s)	177, 1545
Bst4CI	ACNGT	8 cut(s)	667, 733, 799, 964, 1048, 1250, 1262, 1363
Bst6I	CTCTTC	1 cut(s)	1092
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	2 cut(s)	299, 1502
BstBAI	YACGTR	2 cut(s)	1382, 1391
BstC8I	GCNNGC	4 cut(s)	137, 225, 369, 1346
BstDSI	CCRYGG	2 cut(s)	510, 1494
BstF5I	GGATG	3 cut(s)	511, 598, 1354
BstFNI	CGCG	5 cut(s)	59, 512, 1475, 1496, 1587
BstHHI	GCGC	3 cut(s)	61, 1477, 1587
BstKTI	GATC	3 cut(s)	67, 1461, 1543
BstMBI	GATC	3 cut(s)	64, 1458, 1540
BstMCI	CGRYCG	2 cut(s)	368, 702
BstNI	CCWGG	2 cut(s)	177, 1545
BstNSI	RCATGY	2 cut(s)	130, 227
BstSCI	CCNGG	4 cut(s)	175, 1479, 1480, 1543
BstSFI	CTRYAG	3 cut(s)	729, 1029, 1287
BstSLI	GKGCMC	1 cut(s)	1286
BstUI	CGCG	5 cut(s)	59, 512, 1475, 1496, 1587
BstV2I	GAAGAC	3 cut(s)	395, 1097, 1130
BstXI	CCANNNNNNTGG	1 cut(s)	1523
BstZ17I	GTATAC	1 cut(s)	927
BstZI	CGGCCG	2 cut(s)	365, 699
BsuI	GTATCC	1 cut(s)	652
BtgI	CCRYGG	2 cut(s)	510, 1494
BtsCI	GGATG	3 cut(s)	511, 598, 1354
BtsI	GCAGTG	4 cut(s)	408, 630, 1284, 1630
BtsIMutI	CAGTG	7 cut(s)	408, 621, 630, 738, 751, 1284, 1630
Cac8I	GCNNGC	4 cut(s)	137, 225, 369, 1346
CciI	TCATGA	1 cut(s)	984
CfoI	GCGC	3 cut(s)	61, 1477, 1587
Cfr10I	RCCGGY	2 cut(s)	209, 367
Cfr13I	GGNCC	4 cut(s)	783, 1246, 1413, 1618
Cfr42I	CCGCGG	2 cut(s)	513, 1497
Cfr9I	CCCGGG	1 cut(s)	1480
CseI	GACGC	3 cut(s)	242, 400, 1481
Csp6I	GTAC	8 cut(s)	94, 130, 619, 685, 751, 817, 1404, 1439
CspCI	CAANNNNNGTGG	4 cut(s)	958, 993, 1347, 1382
CviAII	CATG	6 cut(s)	127, 155, 224, 254, 985, 1243
CviQI	GTAC	8 cut(s)	94, 130, 619, 685, 751, 817, 1404, 1439
DpnI	GATC	3 cut(s)	66, 1460, 1542
DpnII	GATC	3 cut(s)	64, 1458, 1540
DrdI	GACNNNNNNGTC	1 cut(s)	439
DseDI	GACNNNNNNGTC	1 cut(s)	439
EaeI	YGGCCR	4 cut(s)	133, 365, 699, 1491
EagI	CGGCCG	2 cut(s)	365, 699
Eam1104I	CTCTTC	1 cut(s)	1092
EarI	CTCTTC	1 cut(s)	1092
EciI	GGCGGA	1 cut(s)	530
EclXI	CGGCCG	2 cut(s)	365, 699
Eco130I	CCWWGG	8 cut(s)	435, 447, 501, 645, 654, 720, 786, 1149
Eco24I	GRGCYC	1 cut(s)	984
Eco31I	GGTCTC	3 cut(s)	425, 545, 1160
Eco47I	GGWCC	3 cut(s)	1246, 1413, 1618
Eco52I	CGGCCG	2 cut(s)	365, 699
Eco57I	CTGAAG	1 cut(s)	1116
Eco72I	CACGTG	2 cut(s)	1382, 1391
Eco88I	CYCGRG	1 cut(s)	1480
EcoRII	CCWGG	2 cut(s)	175, 1543
EcoT14I	CCWWGG	8 cut(s)	435, 447, 501, 645, 654, 720, 786, 1149
EcoT38I	GRGCYC	1 cut(s)	984
ErhI	CCWWGG	8 cut(s)	435, 447, 501, 645, 654, 720, 786, 1149
Esp3I	CGTCTC	2 cut(s)	1028, 1148
FaeI	CATG	6 cut(s)	130, 158, 227, 257, 988, 1246
FalI	AAGNNNNNCTT	2 cut(s)	45, 77
FaqI	GGGAC	1 cut(s)	137
FatI	CATG	6 cut(s)	126, 154, 223, 253, 984, 1242
FblI	GTMKAC	1 cut(s)	926
FokI	GGATG	3 cut(s)	518, 605, 1341
FriOI	GRGCYC	1 cut(s)	984
FspBI	CTAG	2 cut(s)	525, 1182
GlaI	GCGC	3 cut(s)	60, 1476, 1586
GsaI	CCCAGC	1 cut(s)	108
GsuI	CTGGAG	2 cut(s)	348, 359
HapII	CCGG	4 cut(s)	210, 368, 1481, 1602
HgaI	GACGC	3 cut(s)	242, 400, 1481
HhaI	GCGC	3 cut(s)	61, 1477, 1587
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	6 cut(s)	130, 158, 227, 257, 988, 1246
Hin6I	GCGC	3 cut(s)	59, 1475, 1585
HinP1I	GCGC	3 cut(s)	59, 1475, 1585
HinfI	GANTC	4 cut(s)	440, 1351, 1376, 1420
HpaII	CCGG	4 cut(s)	210, 368, 1481, 1602
HphI	GGTGA	3 cut(s)	121, 412, 1175
Hpy166II	GTNNAC	6 cut(s)	621, 687, 753, 927, 1594, 1618
Hpy188I	TCNGA	5 cut(s)	69, 265, 286, 639, 1513
Hpy188III	TCNNGA	2 cut(s)	985, 1339
Hpy8I	GTNNAC	6 cut(s)	621, 687, 753, 927, 1594, 1618
HpyCH4III	ACNGT	8 cut(s)	667, 733, 799, 964, 1048, 1250, 1262, 1363
HpyCH4IV	ACGT	3 cut(s)	1157, 1381, 1390
HpyCH4V	TGCA	8 cut(s)	160, 227, 269, 599, 1031, 1289, 1388, 1456
HpySE526I	ACGT	3 cut(s)	1157, 1381, 1390
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	6 cut(s)	130, 158, 227, 257, 988, 1246
HspAI	GCGC	3 cut(s)	59, 1475, 1585
KpnI	GGTACC	1 cut(s)	1442
KroI	GCCGGC	1 cut(s)	367
KroNI	GCCGGC	1 cut(s)	369
KspI	CCGCGG	2 cut(s)	513, 1497
Kzo9I	GATC	3 cut(s)	64, 1458, 1540
LmnI	GCTCC	3 cut(s)	220, 1198, 1579
LweI	GCATC	6 cut(s)	256, 310, 496, 583, 1216, 1321
MaeI	CTAG	2 cut(s)	525, 1182
MaeII	ACGT	3 cut(s)	1157, 1381, 1390
MaeIII	GTNAC	4 cut(s)	205, 1250, 1324, 1377
MalI	GATC	3 cut(s)	66, 1460, 1542
MbiI	CCGCTC	1 cut(s)	1582
MboI	GATC	3 cut(s)	64, 1458, 1540
MboII	GAAGA	7 cut(s)	124, 400, 1022, 1102, 1109, 1135, 1225
MhlI	GDGCHC	2 cut(s)	984, 1286
MluCI	AATT	3 cut(s)	691, 823, 988
MlyI	GAGTC	3 cut(s)	449, 1385, 1414
MmeI	TCCRAC	1 cut(s)	1382
MroNI	GCCGGC	1 cut(s)	367
MroXI	GAANNNNTTC	1 cut(s)	17
MseI	TTAA	2 cut(s)	300, 1503
MslI	CAYNNNNRTG	3 cut(s)	731, 797, 962
MspA1I	CMGCKG	6 cut(s)	104, 512, 920, 932, 1139, 1496
MspCI	CTTAAG	2 cut(s)	299, 1502
MspI	CCGG	4 cut(s)	210, 368, 1481, 1602
MspR9I	CCNGG	4 cut(s)	177, 1481, 1482, 1545
MvaI	CCWGG	2 cut(s)	177, 1545
MvnI	CGCG	5 cut(s)	59, 512, 1475, 1496, 1587
NaeI	GCCGGC	1 cut(s)	369
NciI	CCSGG	2 cut(s)	1481, 1482
NdeII	GATC	3 cut(s)	64, 1458, 1540
NgoMIV	GCCGGC	1 cut(s)	367
NlaIII	CATG	6 cut(s)	130, 158, 227, 257, 988, 1246
NlaIV	GGNNCC	2 cut(s)	784, 1440
NmeAIII	GCCGAG	4 cut(s)	343, 574, 793, 1132
NmuCI	GTSAC	4 cut(s)	205, 1250, 1324, 1377
NspI	RCATGY	2 cut(s)	130, 227
OliI	CACNNNNGTG	3 cut(s)	731, 797, 962
PaeI	GCATGC	1 cut(s)	227
PagI	TCATGA	1 cut(s)	984
PciI	ACATGT	1 cut(s)	126
PcsI	WCGNNNNNNNCGW	3 cut(s)	488, 575, 1387
PdiI	GCCGGC	1 cut(s)	369
PdmI	GAANNNNTTC	1 cut(s)	17
PfeI	GAWTC	1 cut(s)	1351
PleI	GAGTC	3 cut(s)	448, 1384, 1414
PmaCI	CACGTG	2 cut(s)	1382, 1391
PmlI	CACGTG	2 cut(s)	1382, 1391
PpsI	GAGTC	3 cut(s)	448, 1384, 1414
Ppu21I	YACGTR	2 cut(s)	1382, 1391
PscI	ACATGT	1 cut(s)	126
Psp6I	CCWGG	2 cut(s)	175, 1543
PspCI	CACGTG	2 cut(s)	1382, 1391
PspFI	CCCAGC	1 cut(s)	104
PspGI	CCWGG	2 cut(s)	175, 1543
PspN4I	GGNNCC	2 cut(s)	784, 1440
PspPI	GGNCC	4 cut(s)	783, 1246, 1413, 1618
PstI	CTGCAG	2 cut(s)	1033, 1291
PvuII	CAGCTG	3 cut(s)	104, 932, 1139
RsaI	GTAC	8 cut(s)	95, 131, 620, 686, 752, 818, 1405, 1440
RsaNI	GTAC	8 cut(s)	94, 130, 619, 685, 751, 817, 1404, 1439
RseI	CAYNNNNRTG	3 cut(s)	731, 797, 962
SacII	CCGCGG	2 cut(s)	513, 1497
SaqAI	TTAA	2 cut(s)	300, 1503
Sau3AI	GATC	3 cut(s)	64, 1458, 1540
Sau96I	GGNCC	4 cut(s)	783, 1246, 1413, 1618
SchI	GAGTC	3 cut(s)	449, 1385, 1414
ScrFI	CCNGG	4 cut(s)	177, 1481, 1482, 1545
SduI	GDGCHC	2 cut(s)	984, 1286
SfaNI	GCATC	6 cut(s)	256, 310, 496, 583, 1216, 1321
SfcI	CTRYAG	3 cut(s)	729, 1029, 1287
SfiI	GGCCNNNNNGGCC	1 cut(s)	650
Sfr303I	CCGCGG	2 cut(s)	513, 1497
SgrBI	CCGCGG	2 cut(s)	513, 1497
SinI	GGWCC	3 cut(s)	1246, 1413, 1618
SmaI	CCCGGG	1 cut(s)	1482
SmiMI	CAYNNNNRTG	3 cut(s)	731, 797, 962
SmlI	CTYRAG	2 cut(s)	299, 1502
SmoI	CTYRAG	2 cut(s)	299, 1502
SphI	GCATGC	1 cut(s)	227
Sse9I	AATT	3 cut(s)	691, 823, 988
SspMI	CTAG	2 cut(s)	525, 1182
StyD4I	CCNGG	4 cut(s)	175, 1479, 1480, 1543
StyI	CCWWGG	8 cut(s)	435, 447, 501, 645, 654, 720, 786, 1149
TaaI	ACNGT	8 cut(s)	667, 733, 799, 964, 1048, 1250, 1262, 1363
TaiI	ACGT	3 cut(s)	1160, 1384, 1393
TaqI	TCGA	2 cut(s)	11, 1461
TaqII	GACCGA	1 cut(s)	1184
TasI	AATT	3 cut(s)	691, 823, 988
TatI	WGTACW	5 cut(s)	618, 684, 750, 816, 1403
TfiI	GAWTC	1 cut(s)	1351
Tru1I	TTAA	2 cut(s)	300, 1503
Tru9I	TTAA	2 cut(s)	300, 1503
TscAI	CASTG	7 cut(s)	415, 628, 637, 738, 751, 1291, 1630
TseFI	GTSAC	4 cut(s)	205, 1250, 1324, 1377
Tsp45I	GTSAC	4 cut(s)	205, 1250, 1324, 1377
TspDTI	ATGAA	2 cut(s)	1001, 1343
TspGWI	ACGGA	2 cut(s)	584, 1484
TspMI	CCCGGG	1 cut(s)	1480
TspRI	CASTG	7 cut(s)	415, 628, 637, 738, 751, 1291, 1630
Vha464I	CTTAAG	2 cut(s)	299, 1502
VpaK11BI	GGWCC	3 cut(s)	1246, 1413, 1618
XceI	RCATGY	2 cut(s)	130, 227
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1156
XmaI	CCCGGG	1 cut(s)	1480
XmiI	GTMKAC	1 cut(s)	926
XmnI	GAANNNNTTC	1 cut(s)	17
XspI	CTAG	2 cut(s)	525, 1182

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.