AT2G38720

Microtubule associated protein (MAP65/ASE1 family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012187
Physical Location & Seq
Forward (+)
16188047 .. 16192102
4056 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G38720.1

Sequence Viewer

Length: 1653 bp
ATGTCTCCGTCTTCAACCACTACTTGCACTTCCCTTCTTGAAGAGTTGCAGATGATATGGGATGAGATTGGTGAAAGTTACAATGAAAGAGATAAGATGCTGCTAGAACTTGAGCAGGAATGTCTGGATATATACAATAAGAAGGTTGAGAAAACTCGTAAGTTCAGAGCTGAGTTACAGCGATCATTAGCTCAAGCTGAAGCTGAGATTGCCAGCCTCATGTCTGCGCTTGGTGAAAAAGTTTCGTTTGCCAAGAAAGAAGGCTCTTTGAAGGAGCAGATTTCCAGTGTAAAGCCTGTACTGGAAGATCTACTGATGAAAAAGGATCGAAGAAGGAAAGAGCTCTCTGAGACACTGAATCAAATAGCAGAGATCACGTCTAACATAGCAGGGAATGATTATACTGTTAGTTCTGGTTCAGAAGTTGATGAAAGTGACTTGACACAAAGAAAGTTGGATGAACTTAGAGCAGATCTGCAGGATCTTCGAAATGAAAAGGCTGTTCGACTGCAGAAAGTAAACTCTTATATCAGTGCTGTCCATGAGCTGTCAGAGATTCTGTCTTTTGATTTCTCCAAGGCCCTGAATAGTGTCCATAGCAGCTTGACTGAGTTTTCAAAGACTCATTCGAAAAGCATTAGTAATGATACTCTTGCTAGGTTCACAGAACTTGTAAAGTCTCTGAAGGCGGAGAAACATGAGAGGCTGCTTAAGCTACAAGGTTTGGGAAGAAGCATGCAAGAGCTGTGGAACCTAATGGAAACACCTATGGATGAGAGGAGAAGATTTGACCATTGCAGCAGCTTACTTTCTAGTCTACCTGATGATGCCTTGAAAAAAGGATGCCTCAGCCTAGACATTATCCGAGAGGCCGAAGATGAAGTGAGAAGACTGAATTCCCTGAAGTCCAGCAAAATGAAGGAATTGGTGTTTAAGAGGCAGTGTGAACTAGAAGAGATCTGCAGAGGGAATCATATGGATATCAATAGTGATGCTGCAAGAAAGAGTCTTGTCGAACTCATTGAATCTGGTGATGGTGATCTGTCTGATATTCTTGCAAGCATAGATGGTCAGATCGAGAAGGCAAGAGAAGAAGCTTTAAGCAGAAAGGAAATATTGGACAAAGTTGATAAGTGGAGACACGCAAAGGAGGAAGAAACATGGCTTGATGATTATGAAAAGGATGAAAATCGCTTCAGTGCTGTGAGAGGAGCACACAAAAATCTAAAACGGGCAGAAAAAGCTAGGAGCCTTATCAGCAAAATTCCTGCAATGGTGGATGTTCTGACCACGAAAGTGAAGGCCTGGGAGAAAGAAAGAGGGGTCCCCTTCTTATGCGATAAGCAACCGTTATTACAAACACTAGAAGATGACATTGTTATCCGTGCTCAAAGAGAAGAGGAAAAGCGTCAATTCCGAGAGCAGAAACGACTACAAGGTCAGCTTGCTACAGAGAAAGAAGCAAAGTATGGTTCCAAGTCTGCAAAGAAGAAGCCATTGGGACAGAGTCTTAACACAGACAATGTGACTAAAACTCCCATTGGTCGGCGCATTGGAAATACACCTGGACGTTCTGTTACTTCTGGTGGCAAAGATTATAGAGGCAATGCGGTGATACCATTGAACTATGTTGCTCTTCAGAAAGACGATTGA

Protein Analysis

550

Amino Acids

62.65

Weight (kDa)

6.37

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 11 - 499 3.7e-72 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38720
fragaria_vesca FvH4_2g22412 FvH4_7g04100 FvH4_7g04100
malus_domestica MD02G1275300.v1.1 MD07G1043200.v1.1
prunus_persica Prupe.2G045700_v2.0.a1
pyrus_communis pycom07g03050
rosa_chinensis RchiOBHm_Chr1g0327971
rosa_laevigata RLG00000029953
rosa_multiflora Rmu_sc0000675.1_g000047 Rmu_ssc0000467.1_g000036
rosa_roxburghii Rroxscaffold_4G00322110
rosa_rugosa Rorug01G0072700
rosa_samantha Rh1AG088700 Rh1BG071200 Rh1CG086800 Rh1DG093400
rosa_wichuraiana Rw1G006890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1437
AccI GTMKAC 1 cut(s) 817
AciI CCGC 2 cut(s) 689, 1610
AclWI GGATC 2 cut(s) 333, 489
AcsI RAATTY 2 cut(s) 895, 1263
AcuI CTGAAG 5 cut(s) 219, 704, 923, 1180, 1622
AfaI GTAC 1 cut(s) 300
AfiI CCNNNNNNNGG 1 cut(s) 1545
AflII CTTAAG 1 cut(s) 710
AgsI TTSAA 7 cut(s) 15, 41, 271, 618, 835, 1025, 1624
AjiI CACGTC 1 cut(s) 378
AjnI CCWGG 2 cut(s) 1304, 1564
AjuI GAANNNNNNNTTGG 2 cut(s) 1481, 1513
AleI CACNNNNGTG 1 cut(s) 1295
Alw21I GWGCWC 3 cut(s) 345, 1216, 1390
Alw26I GTCTC 4 cut(s) 9, 344, 684, 1132
AlwI GGATC 2 cut(s) 333, 489
AoxI GGCC 3 cut(s) 579, 870, 1302
ApeKI GCWGC 6 cut(s) 100, 600, 706, 798, 801, 995
ApoI RAATTY 2 cut(s) 895, 1263
AspLEI GCGC 2 cut(s) 229, 1551
AspS9I GGNCC 2 cut(s) 580, 1324
AsuHPI GGTGA 5 cut(s) 83, 245, 1043, 1049, 1624
AsuII TTCGAA 2 cut(s) 487, 629
AvaII GGWCC 1 cut(s) 1324
BanII GRGCYC 1 cut(s) 345
BbsI GAAGAC 2 cut(s) 3, 895
Bbv12I GWGCWC 3 cut(s) 345, 1216, 1390
BbvCI CCTCAGC 1 cut(s) 848
BbvI GCAGC 6 cut(s) 87, 612, 693, 810, 813, 982
BccI CCATC 2 cut(s) 1028, 1061
BcgI CGANNNNNNTGC 2 cut(s) 467, 501
BciT130I CCWGG 2 cut(s) 1306, 1566
BcoDI GTCTC 4 cut(s) 9, 344, 684, 1132
BfaI CTAG 7 cut(s) 104, 657, 813, 854, 950, 1245, 1364
BfmI CTRYAG 4 cut(s) 476, 509, 961, 1449
BfrI CTTAAG 1 cut(s) 710
BglII AGATCT 3 cut(s) 307, 472, 957
BisI GCNGC 6 cut(s) 101, 601, 707, 799, 802, 996
BlsI GCNGC 6 cut(s) 102, 602, 708, 800, 803, 997
Bme1390I CCNGG 2 cut(s) 1306, 1566
Bme18I GGWCC 1 cut(s) 1324
BmgBI CACGTC 1 cut(s) 378
BmgT120I GGNCC 2 cut(s) 580, 1324
BmiI GGNNCC 5 cut(s) 752, 1250, 1325, 1326, 1474
BmrFI CCNGG 2 cut(s) 1306, 1566
BmsI GCATC 4 cut(s) 87, 817, 833, 982
BpiI GAAGAC 2 cut(s) 3, 895
Bpu10I CCTNAGC 1 cut(s) 848
Bpu14I TTCGAA 2 cut(s) 487, 629
BpuEI CTTGAG 2 cut(s) 131, 177
BsaBI GATNNNNATC 2 cut(s) 1038, 1188
BsaJI CCNNGG 2 cut(s) 576, 1305
BsaXI ACNNNNNCTCC 2 cut(s) 1519, 1549
Bsc4I CCNNNNNNNGG 1 cut(s) 1545
Bse1I ACTGG 2 cut(s) 285, 306
Bse3DI GCAATG 3 cut(s) 793, 1278, 1612
Bse8I GATNNNNATC 2 cut(s) 1038, 1188
BseBI CCWGG 2 cut(s) 1306, 1566
BseDI CCNNGG 2 cut(s) 576, 1305
BseGI GGATG 6 cut(s) 67, 463, 778, 848, 1189, 1285
BseJI GATNNNNATC 2 cut(s) 1038, 1188
BseLI CCNNNNNNNGG 1 cut(s) 1545
BseMI GCAATG 3 cut(s) 793, 1278, 1612
BseMII CTCAG 5 cut(s) 162, 195, 339, 600, 862
BseNI ACTGG 2 cut(s) 285, 306
BseRI GAGGAG 2 cut(s) 793, 1224
BseXI GCAGC 6 cut(s) 87, 612, 693, 810, 813, 982
BshFI GGCC 3 cut(s) 581, 872, 1304
BsiHKAI GWGCWC 3 cut(s) 345, 1216, 1390
BslFI GGGAC 2 cut(s) 1310, 1515
BslI CCNNNNNNNGG 1 cut(s) 1545
BsmAI GTCTC 4 cut(s) 9, 344, 684, 1132
BsmFI GGGAC 2 cut(s) 1310, 1515
BsnI GGCC 3 cut(s) 581, 872, 1304
Bsp119I TTCGAA 2 cut(s) 487, 629
Bsp1286I GDGCHC 3 cut(s) 345, 1216, 1390
Bsp143I GATC 9 cut(s) 182, 307, 325, 372, 472, 481, 957, 1039, 1074
BspACI CCGC 2 cut(s) 689, 1610
BspANI GGCC 3 cut(s) 581, 872, 1304
BspCNI CTCAG 5 cut(s) 163, 196, 340, 601, 861
BspLI GGNNCC 5 cut(s) 752, 1250, 1325, 1326, 1474
BspMAI CTGCAG 3 cut(s) 480, 513, 965
BspPI GGATC 2 cut(s) 333, 489
BspQI GCTCTTC 1 cut(s) 1641
BspT104I TTCGAA 2 cut(s) 487, 629
BspTI CTTAAG 1 cut(s) 710
BsrDI GCAATG 3 cut(s) 793, 1278, 1612
BsrI ACTGG 2 cut(s) 285, 306
BssECI CCNNGG 2 cut(s) 576, 1305
BssMI GATC 9 cut(s) 182, 307, 325, 372, 472, 481, 957, 1039, 1074
BssT1I CCWWGG 1 cut(s) 576
Bst2UI CCWGG 2 cut(s) 1306, 1566
Bst4CI ACNGT 2 cut(s) 406, 1350
Bst6I CTCTTC 4 cut(s) 36, 948, 1392, 1641
BstAFI CTTAAG 1 cut(s) 710
BstBI TTCGAA 2 cut(s) 487, 629
BstC8I GCNNGC 4 cut(s) 214, 737, 1060, 1446
BstDEI CTNAG 6 cut(s) 171, 204, 348, 464, 609, 848
BstF5I GGATG 6 cut(s) 67, 463, 778, 848, 1189, 1285
BstHHI GCGC 2 cut(s) 229, 1551
BstKTI GATC 9 cut(s) 185, 310, 328, 375, 475, 484, 960, 1042, 1077
BstMAI GTCTC 4 cut(s) 9, 344, 684, 1132
BstMBI GATC 9 cut(s) 182, 307, 325, 372, 472, 481, 957, 1039, 1074
BstMWI GCNNNNNNNGC 4 cut(s) 209, 712, 1241, 1257
BstNI CCWGG 2 cut(s) 1306, 1566
BstNSI RCATGY 1 cut(s) 739
BstSCI CCNGG 2 cut(s) 1304, 1564
BstSFI CTRYAG 4 cut(s) 476, 509, 961, 1449
BstV1I GCAGC 6 cut(s) 87, 612, 693, 810, 813, 982
BstV2I GAAGAC 2 cut(s) 3, 895
BstX2I RGATCY 4 cut(s) 307, 472, 481, 957
BstYI RGATCY 4 cut(s) 307, 472, 481, 957
BsuRI GGCC 3 cut(s) 581, 872, 1304
BtrI CACGTC 1 cut(s) 378
BtsCI GGATG 6 cut(s) 67, 463, 778, 848, 1189, 1285
BtsI GCAGTG 1 cut(s) 947
BtsIMutI CAGTG 5 cut(s) 292, 353, 538, 947, 1204
Cac8I GCNNGC 4 cut(s) 214, 737, 1060, 1446
CfoI GCGC 2 cut(s) 229, 1551
Cfr13I GGNCC 2 cut(s) 580, 1324
CseI GACGC 1 cut(s) 1397
Csp6I GTAC 1 cut(s) 299
CspCI CAANNNNNGTGG 2 cut(s) 728, 763
CviAII CATG 5 cut(s) 220, 542, 698, 736, 1161
CviQI GTAC 1 cut(s) 299
DdeI CTNAG 6 cut(s) 171, 204, 348, 464, 609, 848
DpnI GATC 9 cut(s) 184, 309, 327, 374, 474, 483, 959, 1041, 1076
DpnII GATC 9 cut(s) 182, 307, 325, 372, 472, 481, 957, 1039, 1074
DrdI GACNNNNNNGTC 1 cut(s) 1437
DseDI GACNNNNNNGTC 1 cut(s) 1437
Eam1104I CTCTTC 4 cut(s) 36, 948, 1392, 1641
EarI CTCTTC 4 cut(s) 36, 948, 1392, 1641
EciI GGCGGA 1 cut(s) 704
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 576
Eco147I AGGCCT 1 cut(s) 1304
Eco24I GRGCYC 1 cut(s) 345
Eco32I GATATC 1 cut(s) 982
Eco47I GGWCC 1 cut(s) 1324
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 5 cut(s) 219, 704, 923, 1180, 1622
EcoICRI GAGCTC 1 cut(s) 343
EcoO109I RGGNCCY 2 cut(s) 580, 1324
EcoRI GAATTC 1 cut(s) 895
EcoRII CCWGG 2 cut(s) 1304, 1564
EcoRV GATATC 1 cut(s) 982
EcoT14I CCWWGG 1 cut(s) 576
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 576
FaeI CATG 5 cut(s) 223, 545, 701, 739, 1164
FalI AAGNNNNNCTT 2 cut(s) 1428, 1460
FaqI GGGAC 2 cut(s) 1310, 1515
FatI CATG 5 cut(s) 219, 541, 697, 735, 1160
FauNDI CATATG 1 cut(s) 975
FblI GTMKAC 1 cut(s) 817
Fnu4HI GCNGC 6 cut(s) 101, 601, 707, 799, 802, 996
FokI GGATG 6 cut(s) 74, 470, 785, 855, 1196, 1292
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 6 cut(s) 101, 601, 707, 799, 802, 996
FspBI CTAG 7 cut(s) 104, 657, 813, 854, 950, 1245, 1364
GlaI GCGC 2 cut(s) 228, 1550
GluI GCNGC 6 cut(s) 101, 601, 707, 799, 802, 996
HaeIII GGCC 3 cut(s) 581, 872, 1304
HgaI GACGC 1 cut(s) 1397
HhaI GCGC 2 cut(s) 229, 1551
Hin1II CATG 5 cut(s) 223, 545, 701, 739, 1164
Hin6I GCGC 2 cut(s) 227, 1549
HinP1I GCGC 2 cut(s) 227, 1549
HindIII AAGCTT 1 cut(s) 1095
HinfI GANTC 7 cut(s) 358, 556, 622, 970, 1006, 1025, 1507
HphI GGTGA 5 cut(s) 83, 245, 1043, 1049, 1624
Hpy166II GTNNAC 4 cut(s) 520, 663, 818, 947
Hpy188III TCNNGA 3 cut(s) 38, 125, 1078
Hpy8I GTNNAC 4 cut(s) 520, 663, 818, 947
HpyCH4III ACNGT 2 cut(s) 406, 1350
HpyCH4IV ACGT 2 cut(s) 377, 1570
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 712, 1241, 1257
HpyF3I CTNAG 6 cut(s) 171, 204, 348, 464, 609, 848
HpySE526I ACGT 2 cut(s) 377, 1570
Hsp92II CATG 5 cut(s) 223, 545, 701, 739, 1164
HspAI GCGC 2 cut(s) 227, 1549
KflI GGGWCCC 1 cut(s) 1324
Kzo9I GATC 9 cut(s) 182, 307, 325, 372, 472, 481, 957, 1039, 1074
LguI GCTCTTC 1 cut(s) 1641
LmnI GCTCC 3 cut(s) 274, 1211, 1248
Lsp1109I GCAGC 6 cut(s) 87, 612, 693, 810, 813, 982
LweI GCATC 4 cut(s) 87, 817, 833, 982
MaeI CTAG 7 cut(s) 104, 657, 813, 854, 950, 1245, 1364
MaeII ACGT 2 cut(s) 377, 1570
MaeIII GTNAC 5 cut(s) 77, 174, 434, 1525, 1576
MalI GATC 9 cut(s) 184, 309, 327, 374, 474, 483, 959, 1041, 1076
MboI GATC 9 cut(s) 182, 307, 325, 372, 472, 481, 957, 1039, 1074
MflI RGATCY 4 cut(s) 307, 472, 481, 957
MhlI GDGCHC 3 cut(s) 345, 1216, 1390
MluCI AATT 4 cut(s) 895, 923, 1263, 1412
MlyI GAGTC 3 cut(s) 616, 1015, 1516
MmeI TCCRAC 1 cut(s) 435
MseI TTAA 4 cut(s) 711, 933, 1100, 1512
MslI CAYNNNNRTG 1 cut(s) 1295
MspCI CTTAAG 1 cut(s) 710
MspR9I CCNGG 2 cut(s) 1306, 1566
MvaI CCWGG 2 cut(s) 1306, 1566
MwoI GCNNNNNNNGC 4 cut(s) 209, 712, 1241, 1257
NdeI CATATG 1 cut(s) 975
NdeII GATC 9 cut(s) 182, 307, 325, 372, 472, 481, 957, 1039, 1074
NlaIII CATG 5 cut(s) 223, 545, 701, 739, 1164
NlaIV GGNNCC 5 cut(s) 752, 1250, 1325, 1326, 1474
NmuCI GTSAC 2 cut(s) 434, 1525
NspI RCATGY 1 cut(s) 739
NspV TTCGAA 2 cut(s) 487, 629
OliI CACNNNNGTG 1 cut(s) 1295
PaeI GCATGC 1 cut(s) 739
PceI AGGCCT 1 cut(s) 1304
PciSI GCTCTTC 1 cut(s) 1641
PfeI GAWTC 4 cut(s) 358, 556, 970, 1025
PflFI GACNNNGTC 1 cut(s) 1506
PkrI GCNGC 6 cut(s) 102, 602, 708, 800, 803, 997
PleI GAGTC 3 cut(s) 616, 1014, 1515
PpsI GAGTC 3 cut(s) 616, 1014, 1515
PpuMI RGGWCCY 1 cut(s) 1324
Psp124BI GAGCTC 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 1324
Psp6I CCWGG 2 cut(s) 1304, 1564
PspGI CCWGG 2 cut(s) 1304, 1564
PspN4I GGNNCC 5 cut(s) 752, 1250, 1325, 1326, 1474
PspPI GGNCC 2 cut(s) 580, 1324
PspPPI RGGWCCY 1 cut(s) 1324
PstI CTGCAG 3 cut(s) 480, 513, 965
PsuI RGATCY 4 cut(s) 307, 472, 481, 957
PsyI GACNNNGTC 1 cut(s) 1506
RsaI GTAC 1 cut(s) 300
RsaNI GTAC 1 cut(s) 299
RseI CAYNNNNRTG 1 cut(s) 1295
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 1641
SaqAI TTAA 4 cut(s) 711, 933, 1100, 1512
SatI GCNGC 6 cut(s) 101, 601, 707, 799, 802, 996
Sau3AI GATC 9 cut(s) 182, 307, 325, 372, 472, 481, 957, 1039, 1074
Sau96I GGNCC 2 cut(s) 580, 1324
SchI GAGTC 3 cut(s) 616, 1015, 1516
ScrFI CCNGG 2 cut(s) 1306, 1566
SduI GDGCHC 3 cut(s) 345, 1216, 1390
SfaNI GCATC 4 cut(s) 87, 817, 833, 982
SfcI CTRYAG 4 cut(s) 476, 509, 961, 1449
SfuI TTCGAA 2 cut(s) 487, 629
SinI GGWCC 1 cut(s) 1324
SmiMI CAYNNNNRTG 1 cut(s) 1295
SmlI CTYRAG 3 cut(s) 110, 192, 710
SmoI CTYRAG 3 cut(s) 110, 192, 710
SphI GCATGC 1 cut(s) 739
Sse9I AATT 4 cut(s) 895, 923, 1263, 1412
SseBI AGGCCT 1 cut(s) 1304
SsiI CCGC 2 cut(s) 689, 1610
SspI AATATT 1 cut(s) 1116
SspMI CTAG 7 cut(s) 104, 657, 813, 854, 950, 1245, 1364
SstI GAGCTC 1 cut(s) 345
StuI AGGCCT 1 cut(s) 1304
StyD4I CCNGG 2 cut(s) 1304, 1564
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 2 cut(s) 406, 1350
TaiI ACGT 2 cut(s) 380, 1573
TaqI TCGA 6 cut(s) 328, 487, 505, 629, 1014, 1077
TasI AATT 4 cut(s) 895, 923, 1263, 1412
TatI WGTACW 1 cut(s) 298
TfiI GAWTC 4 cut(s) 358, 556, 970, 1025
Tru1I TTAA 4 cut(s) 711, 933, 1100, 1512
Tru9I TTAA 4 cut(s) 711, 933, 1100, 1512
TscAI CASTG 5 cut(s) 292, 360, 538, 947, 1204
TseFI GTSAC 2 cut(s) 434, 1525
TseI GCWGC 6 cut(s) 100, 600, 706, 798, 801, 995
Tsp45I GTSAC 2 cut(s) 434, 1525
TspDTI ATGAA 9 cut(s) 99, 332, 444, 474, 507, 894, 932, 1191, 1200
TspGWI ACGGA 1 cut(s) 1373
TspRI CASTG 5 cut(s) 292, 360, 538, 947, 1204
Tth111I GACNNNGTC 1 cut(s) 1506
Vha464I CTTAAG 1 cut(s) 710
VpaK11BI GGWCC 1 cut(s) 1324
XapI RAATTY 2 cut(s) 895, 1263
XceI RCATGY 1 cut(s) 739
XmiI GTMKAC 1 cut(s) 817
XspI CTAG 7 cut(s) 104, 657, 813, 854, 950, 1245, 1364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.