RLG00000029953

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012187
Physical Location & Seq
Forward (+)
52057135 .. 52062448
5314 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029953

Sequence Viewer

Length: 1584 bp
ATGTCGGCGGTGCCGCCGCCCGTTTCTCCCTCCCGCACCACCTGCGCTTCTCTCCTCCATGAACTTCAGATCATTTGGGACGAAATTGGGGAGAGTGACAGTGAGAGAGACACCATGCTTCTCCAACTCGAGCAAGAATGCCTCGACATCTATCGGAAAAAGGTGGAAATGACGAGAAAGTACAGGGCTGATTTGCACAGGTCCCTCGCCGACGGCCAAGCTCAAATCGCAGCCATCGCCTCCGCTCTCGGAGAACCTAATTCGTTTCCTAGGGCAAGAGGGACCCTCAGGGACCAGATATCCGCCGTGAAGCCTGTTTTGGACGAACTGAGCTTGAAGAAGCAGGAAAGGGTGAGGGACTTTTCGGTAATTCAAACACAGATTGCGAGGATTTCGTCTGAAATAGCCGGGAACGGCGTGGCTAAGAATGCCGGTGTGGATGCCGGAGTGGATGAGCGTGATTTGACTGTGAAGAGGTTGGGAGAGCTTAAGTCCCACCTTGACGAACTTCATAAAGAAAAGGCTTTACGTTTACAGAAAGTCAATAGTCATGTCAATACGATCCATGACCTCTCAGTTGTAATGTCCATTGATTTCTTTAAGACACTCGAAGATGTCCACCCAAGCTTGAGTGATCAGTCCGCCGCTCATTCCAAGAGCATCAGCAATGACACTCTTGCTAGATTGACCGCGCTGACCAACTCGCTGAAGCAAGAGAAACAGCACAGACTGCAAAAGCTACAAGAGCTTGGAAGCAAGCTGATAGAAGTATGGAATCTCATGGAGACACCAACTGAGGAACGGAACAAATTTGGCCATGTTACGCGCTTAGTTTCTTCCTCAGTTGATGAGCTGTCAAGGCATGGATGCCTTTCTCCAGATGTCATTGAGCAGACTGAGGCTGAAGTCGAGCGGTTGAATGTTTTGAAAGCCAGTAATGTTAATTTGTCTGATCTGCTTTCAAGCATGGATGATCAGATTGTAAAGGCAAAAGAGCAAGCTTTAAGCAGAAAGGATATTTTGGATAAGGTAGAGAAATGCAAATTTGCATTTGAGGAGGATAAGTGGCTTGATGAGTATGAAAAGGATGAAAATCGATATAGTGCAGGAAGAGGAGCACACAAAAATTTGAAGCGTGCTGAGAAAGCCCGGATTTTGGTTGGAAAAATACCATCTATCATTGAAAGTCTGACTGCCAGAGTTAAAGCATGGGAGGGCGAGAAAGGAGTCCCTTTCTTATACGACAAGGTTCCCCTTTTACATATCGTGGAGGAGTACGCTGCACAACGGCAGGAGAGAGAGGAGGAAAAACGTAAATCTCGGGAGCAAAAACGTCTGCAAGAACAACATGCGACGGAACAGGAGGCACTATTTGGTTCAAGGCCCTCTACAAAGAAGCCATTGGGACAAAGCACCAATGCCAATACCATGATTGGGACCCCAATTAGTCGTCGTACAAGTACCCCTTCGGGCCGTCATGGAGTGTCAGCTGCAAAAGAACGCAGAGAAAGTGGGAGGGCCAACATAACACTTGTTAACTATGTTGCTCTTCCAAAGGATGATTCTGTTGCTCGGAGAAATTGA

Protein Analysis

528

Amino Acids

59.27

Weight (kDa)

8.15

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 17 - 314 9.2e-37 Microtubule associated protein (MAP65/ASE1 family)
MAP65_ASE1 PF03999 318 - 467 6.6e-29 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38720
fragaria_vesca FvH4_2g22412 FvH4_7g04100 FvH4_7g04100
malus_domestica MD02G1275300.v1.1 MD07G1043200.v1.1
prunus_persica Prupe.2G045700_v2.0.a1
pyrus_communis pycom07g03050
rosa_chinensis RchiOBHm_Chr1g0327971
rosa_laevigata RLG00000029953
rosa_multiflora Rmu_sc0000675.1_g000047 Rmu_ssc0000467.1_g000036
rosa_roxburghii Rroxscaffold_4G00322110
rosa_rugosa Rorug01G0072700
rosa_samantha Rh1AG088700 Rh1BG071200 Rh1CG086800 Rh1DG093400
rosa_wichuraiana Rw1G006890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 50
Acc36I ACCTGC 1 cut(s) 50
AccB1I GGYRCC 1 cut(s) 10
AccBSI CCGCTC 3 cut(s) 245, 647, 913
AccII CGCG 2 cut(s) 692, 826
AclWI GGATC 1 cut(s) 556
AcoI YGGCCR 2 cut(s) 214, 814
AcsI RAATTY 3 cut(s) 809, 1043, 1126
AcuI CTGAAG 3 cut(s) 50, 728, 924
AfaI GTAC 4 cut(s) 182, 1277, 1456, 1462
AfiI CCNNNNNNNGG 2 cut(s) 1156, 1434
AflII CTTAAG 1 cut(s) 488
AgsI TTSAA 8 cut(s) 337, 374, 919, 928, 963, 1132, 1184, 1380
AjuI GAANNNNNNNTTGG 4 cut(s) 302, 334, 1546, 1578
Alw21I GWGCWC 1 cut(s) 1120
Alw26I GTCTC 2 cut(s) 102, 779
AlwI GGATC 1 cut(s) 556
Ama87I CYCGRG 2 cut(s) 128, 1320
AoxI GGCC 5 cut(s) 214, 814, 1382, 1471, 1518
ApeKI GCWGC 3 cut(s) 230, 1280, 1490
ApoI RAATTY 3 cut(s) 809, 1043, 1126
AspA2I CCTAGG 1 cut(s) 269
AspLEI GCGC 3 cut(s) 47, 694, 828
AspS9I GGNCC 7 cut(s) 201, 282, 292, 1383, 1437, 1471, 1518
AsuC2I CCSGG 2 cut(s) 409, 1150
AsuHPI GGTGA 1 cut(s) 364
AvaI CYCGRG 2 cut(s) 128, 1320
AvaII GGWCC 4 cut(s) 201, 282, 292, 1437
AvrII CCTAGG 1 cut(s) 269
AxyI CCTNAGG 1 cut(s) 287
BalI TGGCCA 1 cut(s) 816
BanI GGYRCC 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 1120
BbvI GCAGC 3 cut(s) 242, 1267, 1477
BccI CCATC 2 cut(s) 242, 1180
BceAI ACGGC 5 cut(s) 229, 290, 430, 1304, 1458
BclI TGATCA 2 cut(s) 634, 973
BcnI CCSGG 2 cut(s) 409, 1150
BcoDI GTCTC 2 cut(s) 102, 779
BfaI CTAG 2 cut(s) 270, 681
BfrI CTTAAG 1 cut(s) 488
BfuAI ACCTGC 1 cut(s) 50
BisI GCNGC 6 cut(s) 14, 17, 231, 645, 1281, 1491
BlnI CCTAGG 1 cut(s) 269
BlsI GCNGC 6 cut(s) 15, 18, 232, 646, 1282, 1492
Bme1390I CCNGG 2 cut(s) 409, 1150
Bme18I GGWCC 4 cut(s) 201, 282, 292, 1437
BmeT110I CYCGRG 2 cut(s) 128, 1320
BmgT120I GGNCC 7 cut(s) 201, 282, 292, 1383, 1437, 1471, 1518
BmiI GGNNCC 8 cut(s) 12, 203, 283, 284, 293, 1251, 1438, 1439
BmrFI CCNGG 2 cut(s) 409, 1150
BmsI GCATC 3 cut(s) 430, 669, 857
BplI GAGNNNNNCTC 2 cut(s) 270, 302
BpmI CTGGAG 1 cut(s) 861
BpuEI CTTGAG 1 cut(s) 649
BpuMI CCSGG 2 cut(s) 409, 1150
Bsa29I ATCGAT 1 cut(s) 1096
BsaBI GATNNNNATC 1 cut(s) 1092
BsaJI CCNNGG 1 cut(s) 269
BsaXI ACNNNNNCTCC 2 cut(s) 1049, 1079
Bsc4I CCNNNNNNNGG 2 cut(s) 1156, 1434
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 1 cut(s) 933
Bse21I CCTNAGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 673
Bse8I GATNNNNATC 1 cut(s) 1092
BseCI ATCGAT 1 cut(s) 1096
BseDI CCNNGG 1 cut(s) 269
BseGI GGATG 6 cut(s) 445, 457, 872, 976, 1093, 1564
BseJI GATNNNNATC 1 cut(s) 1092
BseLI CCNNNNNNNGG 2 cut(s) 1156, 1434
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 7 cut(s) 301, 320, 588, 786, 855, 888, 1131
BseNI ACTGG 1 cut(s) 933
BseRI GAGGAG 5 cut(s) 44, 1070, 1128, 1286, 1316
BseXI GCAGC 3 cut(s) 242, 1267, 1477
BsgI GTGCAG 2 cut(s) 1125, 1266
Bsh1236I CGCG 2 cut(s) 692, 826
BshFI GGCC 5 cut(s) 216, 816, 1384, 1473, 1520
BshNI GGYRCC 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 1096
BsiHKAI GWGCWC 1 cut(s) 1120
BsiHKCI CYCGRG 2 cut(s) 128, 1320
BsiSI CCGG 4 cut(s) 408, 432, 444, 1150
BslFI GGGAC 9 cut(s) 92, 187, 295, 305, 371, 478, 1214, 1419, 1450
BslI CCNNNNNNNGG 2 cut(s) 1156, 1434
BsmAI GTCTC 2 cut(s) 102, 779
BsmFI GGGAC 9 cut(s) 92, 187, 295, 305, 371, 478, 1214, 1419, 1450
BsmI GAATGC 2 cut(s) 143, 433
BsnI GGCC 5 cut(s) 216, 816, 1384, 1473, 1520
BsoBI CYCGRG 2 cut(s) 128, 1320
Bsp1286I GDGCHC 1 cut(s) 1120
Bsp143I GATC 5 cut(s) 69, 561, 634, 952, 973
BspANI GGCC 5 cut(s) 216, 816, 1384, 1473, 1520
BspCNI CTCAG 7 cut(s) 300, 321, 587, 787, 854, 889, 1132
BspDI ATCGAT 1 cut(s) 1096
BspFNI CGCG 2 cut(s) 692, 826
BspLI GGNNCC 8 cut(s) 12, 203, 283, 284, 293, 1251, 1438, 1439
BspMI ACCTGC 1 cut(s) 50
BspPI GGATC 1 cut(s) 556
BspQI GCTCTTC 1 cut(s) 1554
BspT107I GGYRCC 1 cut(s) 10
BspTI CTTAAG 1 cut(s) 488
BsrBI CCGCTC 3 cut(s) 245, 647, 913
BsrDI GCAATG 1 cut(s) 673
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 1 cut(s) 933
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 1 cut(s) 269
BssMI GATC 5 cut(s) 69, 561, 634, 952, 973
BssT1I CCWWGG 1 cut(s) 269
Bst4CI ACNGT 2 cut(s) 101, 469
Bst6I CTCTTC 3 cut(s) 467, 1105, 1554
BstAFI CTTAAG 1 cut(s) 488
BstAPI GCANNNNNTGC 2 cut(s) 42, 730
BstC8I GCNNGC 3 cut(s) 758, 999, 1137
BstDEI CTNAG 9 cut(s) 287, 329, 423, 574, 795, 829, 841, 897, 1140
BstF5I GGATG 6 cut(s) 445, 457, 872, 976, 1093, 1564
BstFNI CGCG 2 cut(s) 692, 826
BstHHI GCGC 3 cut(s) 47, 694, 828
BstKTI GATC 5 cut(s) 72, 564, 637, 955, 976
BstMAI GTCTC 2 cut(s) 102, 779
BstMBI GATC 5 cut(s) 69, 561, 634, 952, 973
BstMWI GCNNNNNNNGC 8 cut(s) 42, 227, 236, 428, 730, 745, 859, 1145
BstNSI RCATGY 1 cut(s) 1352
BstSCI CCNGG 2 cut(s) 407, 1148
BstUI CGCG 2 cut(s) 692, 826
BstV1I GCAGC 3 cut(s) 242, 1267, 1477
Bsu15I ATCGAT 1 cut(s) 1096
Bsu36I CCTNAGG 1 cut(s) 287
BsuRI GGCC 5 cut(s) 216, 816, 1384, 1473, 1520
BsuTUI ATCGAT 1 cut(s) 1096
BtgZI GCGATG 1 cut(s) 220
BtsCI GGATG 6 cut(s) 445, 457, 872, 976, 1093, 1564
BtsIMutI CAGTG 1 cut(s) 106
BveI ACCTGC 1 cut(s) 50
Cac8I GCNNGC 3 cut(s) 758, 999, 1137
CfoI GCGC 3 cut(s) 47, 694, 828
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 7 cut(s) 201, 282, 292, 1383, 1437, 1471, 1518
ClaI ATCGAT 1 cut(s) 1096
Csp6I GTAC 4 cut(s) 181, 1276, 1455, 1461
CviQI GTAC 4 cut(s) 181, 1276, 1455, 1461
DdeI CTNAG 9 cut(s) 287, 329, 423, 574, 795, 829, 841, 897, 1140
DpnI GATC 5 cut(s) 71, 563, 636, 954, 975
DpnII GATC 5 cut(s) 69, 561, 634, 952, 973
EaeI YGGCCR 2 cut(s) 214, 814
Eam1104I CTCTTC 3 cut(s) 467, 1105, 1554
EarI CTCTTC 3 cut(s) 467, 1105, 1554
EciI GGCGGA 2 cut(s) 292, 631
Eco130I CCWWGG 1 cut(s) 269
Eco32I GATATC 1 cut(s) 300
Eco47I GGWCC 4 cut(s) 201, 282, 292, 1437
Eco57I CTGAAG 3 cut(s) 50, 728, 924
Eco81I CCTNAGG 1 cut(s) 287
Eco88I CYCGRG 2 cut(s) 128, 1320
EcoO109I RGGNCCY 4 cut(s) 201, 282, 1383, 1437
EcoRV GATATC 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 269
FalI AAGNNNNNCTT 2 cut(s) 1450, 1482
FaqI GGGAC 9 cut(s) 92, 187, 295, 305, 371, 478, 1214, 1419, 1450
FauI CCCGC 1 cut(s) 41
FbaI TGATCA 2 cut(s) 634, 973
Fnu4HI GCNGC 6 cut(s) 14, 17, 231, 645, 1281, 1491
FokI GGATG 6 cut(s) 452, 464, 879, 983, 1100, 1571
Fsp4HI GCNGC 6 cut(s) 14, 17, 231, 645, 1281, 1491
FspBI CTAG 2 cut(s) 270, 681
GlaI GCGC 3 cut(s) 46, 693, 827
GluI GCNGC 6 cut(s) 14, 17, 231, 645, 1281, 1491
GsuI CTGGAG 1 cut(s) 861
HaeIII GGCC 5 cut(s) 216, 816, 1384, 1473, 1520
HapII CCGG 4 cut(s) 408, 432, 444, 1150
HhaI GCGC 3 cut(s) 47, 694, 828
Hin6I GCGC 3 cut(s) 45, 692, 826
HinP1I GCGC 3 cut(s) 45, 692, 826
HincII GTYRAC 1 cut(s) 1537
HindII GTYRAC 1 cut(s) 1537
HindIII AAGCTT 2 cut(s) 625, 999
HinfI GANTC 3 cut(s) 775, 1227, 1562
HpaI GTTAAC 1 cut(s) 1537
HpaII CCGG 4 cut(s) 408, 432, 444, 1150
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 3 cut(s) 533, 619, 1537
Hpy188I TCNGA 8 cut(s) 69, 156, 251, 400, 952, 978, 1191, 1575
Hpy188III TCNNGA 2 cut(s) 878, 1322
Hpy8I GTNNAC 3 cut(s) 533, 619, 1537
Hpy99I CGWCG 3 cut(s) 215, 1357, 1455
HpyAV CCTTC 1 cut(s) 1476
HpyCH4III ACNGT 2 cut(s) 101, 469
HpyCH4IV ACGT 3 cut(s) 529, 1312, 1333
HpyCH4V TGCA 8 cut(s) 196, 733, 1041, 1049, 1106, 1283, 1339, 1493
HpyF10VI GCNNNNNNNGC 8 cut(s) 42, 227, 236, 428, 730, 745, 859, 1145
HpyF3I CTNAG 9 cut(s) 287, 329, 423, 574, 795, 829, 841, 897, 1140
HpySE526I ACGT 3 cut(s) 529, 1312, 1333
HspAI GCGC 3 cut(s) 45, 692, 826
KflI GGGWCCC 2 cut(s) 282, 1437
Ksp22I TGATCA 2 cut(s) 634, 973
KspAI GTTAAC 1 cut(s) 1537
Kzo9I GATC 5 cut(s) 69, 561, 634, 952, 973
LguI GCTCTTC 1 cut(s) 1554
LmnI GCTCC 2 cut(s) 1115, 1324
Lsp1109I GCAGC 3 cut(s) 242, 1267, 1477
LweI GCATC 3 cut(s) 430, 669, 857
MaeI CTAG 2 cut(s) 270, 681
MaeII ACGT 3 cut(s) 529, 1312, 1333
MaeIII GTNAC 2 cut(s) 95, 820
MalI GATC 5 cut(s) 71, 563, 636, 954, 975
MbiI CCGCTC 3 cut(s) 245, 647, 913
MboI GATC 5 cut(s) 69, 561, 634, 952, 973
MboII GAAGA 6 cut(s) 349, 484, 623, 828, 1122, 1541
MhlI GDGCHC 1 cut(s) 1120
MlsI TGGCCA 1 cut(s) 816
MluCI AATT 9 cut(s) 84, 259, 369, 809, 943, 1043, 1126, 1443, 1579
MluNI TGGCCA 1 cut(s) 816
MlyI GAGTC 1 cut(s) 1236
MmeI TCCRAC 2 cut(s) 148, 1141
Mox20I TGGCCA 1 cut(s) 816
MscI TGGCCA 1 cut(s) 816
MseI TTAA 6 cut(s) 489, 600, 942, 1004, 1203, 1536
Msp20I TGGCCA 1 cut(s) 816
MspA1I CMGCKG 1 cut(s) 1490
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 4 cut(s) 408, 432, 444, 1150
MspR9I CCNGG 2 cut(s) 409, 1150
Mva1269I GAATGC 2 cut(s) 143, 433
MvnI CGCG 2 cut(s) 692, 826
MwoI GCNNNNNNNGC 8 cut(s) 42, 227, 236, 428, 730, 745, 859, 1145
NciI CCSGG 2 cut(s) 409, 1150
NdeII GATC 5 cut(s) 69, 561, 634, 952, 973
NlaIV GGNNCC 8 cut(s) 12, 203, 283, 284, 293, 1251, 1438, 1439
NmuCI GTSAC 1 cut(s) 95
NspI RCATGY 1 cut(s) 1352
PaeR7I CTCGAG 1 cut(s) 128
PaqCI CACCTGC 1 cut(s) 50
PciSI GCTCTTC 1 cut(s) 1554
PctI GAATGC 2 cut(s) 143, 433
PfeI GAWTC 2 cut(s) 775, 1562
PkrI GCNGC 6 cut(s) 15, 18, 232, 646, 1282, 1492
PleI GAGTC 1 cut(s) 1235
PpsI GAGTC 1 cut(s) 1235
PpuMI RGGWCCY 3 cut(s) 201, 282, 1437
Psp5II RGGWCCY 3 cut(s) 201, 282, 1437
PspN4I GGNNCC 8 cut(s) 12, 203, 283, 284, 293, 1251, 1438, 1439
PspPI GGNCC 7 cut(s) 201, 282, 292, 1383, 1437, 1471, 1518
PspPPI RGGWCCY 3 cut(s) 201, 282, 1437
PspXI VCTCGAGB 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 1490
RsaI GTAC 4 cut(s) 182, 1277, 1456, 1462
RsaNI GTAC 4 cut(s) 181, 1276, 1455, 1461
SapI GCTCTTC 1 cut(s) 1554
SaqAI TTAA 6 cut(s) 489, 600, 942, 1004, 1203, 1536
SatI GCNGC 6 cut(s) 14, 17, 231, 645, 1281, 1491
Sau3AI GATC 5 cut(s) 69, 561, 634, 952, 973
Sau96I GGNCC 7 cut(s) 201, 282, 292, 1383, 1437, 1471, 1518
SchI GAGTC 1 cut(s) 1236
ScrFI CCNGG 2 cut(s) 409, 1150
SduI GDGCHC 1 cut(s) 1120
SfaNI GCATC 3 cut(s) 430, 669, 857
Sfr274I CTCGAG 1 cut(s) 128
SinI GGWCC 4 cut(s) 201, 282, 292, 1437
SlaI CTCGAG 1 cut(s) 128
SmlI CTYRAG 3 cut(s) 128, 488, 628
SmoI CTYRAG 3 cut(s) 128, 488, 628
Sse9I AATT 9 cut(s) 84, 259, 369, 809, 943, 1043, 1126, 1443, 1579
SspMI CTAG 2 cut(s) 270, 681
StyD4I CCNGG 2 cut(s) 407, 1148
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 2 cut(s) 101, 469
TaiI ACGT 3 cut(s) 532, 1315, 1336
TaqI TCGA 5 cut(s) 129, 144, 609, 909, 1096
TasI AATT 9 cut(s) 84, 259, 369, 809, 943, 1043, 1126, 1443, 1579
TatI WGTACW 1 cut(s) 180
TauI GCSGC 3 cut(s) 16, 19, 647
TfiI GAWTC 2 cut(s) 775, 1562
Tru1I TTAA 6 cut(s) 489, 600, 942, 1004, 1203, 1536
Tru9I TTAA 6 cut(s) 489, 600, 942, 1004, 1203, 1536
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 3 cut(s) 230, 1280, 1490
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 4 cut(s) 75, 500, 1095, 1104
TspGWI ACGGA 2 cut(s) 817, 1370
TspRI CASTG 1 cut(s) 106
Vha464I CTTAAG 1 cut(s) 488
VpaK11BI GGWCC 4 cut(s) 201, 282, 292, 1437
XapI RAATTY 3 cut(s) 809, 1043, 1126
XceI RCATGY 1 cut(s) 1352
XhoI CTCGAG 1 cut(s) 128
XmaJI CCTAGG 1 cut(s) 269
XspI CTAG 2 cut(s) 270, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.