Rh1BG071200

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0012187
Physical Location & Seq
Reverse (-)
10864726 .. 10870985
6260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG071200.1

Sequence Viewer

Length: 1704 bp
ATGTCGGCGGTGCCGCCGCCCGTTTCTCCCTCCCGCACCACCTGCGCTTCTCTCCTCCATGAATTACAGATCATTTGGGACGAAATTGGGGAGAGTGACAGTGAGAGGGACACCATGCTTCTCCAACTCGAGCAAGAGTGCCTCGACATCTATCGGAAGAAGGTGGAAATGACGAGAAAGTACAGGGCTGATTTGCACAAGTCCTTAGCAGACGGCCAAGCTCAAATCGCCGCCATCGCCTCCGCTCTCGGAGAACCTAATTCGTTTCCAAGGGCACGAGGGACCCTCAGGGACCAGATATCCGCCGTGAAGCCTGTTTTGGACGAACTGAGCTTGAAGAAGCAGGAAAGGGTGAGGGACTTTTCGGTAATTCAAACACAGATTGCGAGGATTTCGTCTGAAATAGCCGGGAACGGCCTGGCTAAGAATGCCGGTGAGGATGCCGGAGTCGATGAGCGTGATTTGACTGTGAAGAGGTTGGGAGAGCTTAAGTCCCACCTTGACGAACTTCACAAAGAAAAGGCTTTACGTTTACAGAAGGTCAATAGTCATGTCAATACCATCCATGACCTCTCAGTTGTAATGTCCATTGATTTCTTTAGGACACTCGAAGAAGTCCACCCAAGCTTGAGTGATCAGTCCGCCGCTCATTCCAAGAGCATCAGCAATGACACTCTTGCTAGATTGACCGCGCTGACCAACTCGCTGAAGCAAGAGAAACAGCAAAGACTGCAAAAGCTACAAGAGCTTGGAAGCAAGCTGATAGAAGTATGGAATCTCATGGAGACACCAACTGAGGAACGGAACAAATTTGACCATGTTACGCGCTTAATTTCTTCCTCAGTTGATGAGCTGTCAAGGCATGGATGCCTTTCTCTAGATGTCATTGAGCAGACTGAGGCTGAAGTCGAGCGGTTGAATGTTTTGAAAGCCAGCAAGATGAAGGAGTTGGTCTTTAAGAGGCAAAATGAGCTCGAAGAAATTTATAGAGGAGTCCACATGGATGTAGATAGTGATGGAGCTAGACAGATTCTCAGCAGCCTCATAGATTCTGGTAATGTTAATTTGTCTGATCTGCTTTCAAGCATGGATGATCAGATTGTAAAGGCAAAAGAGCAAGCTTTAAGCAGAAAGGATATTTTGGATAAGGTAGAGAAATGCAAATTTGCATTTGAGGAGGATAAGTGGCTTGATGAGTATGAAAAGGATGAAAATCGATATAGTGCAGGAAGAGGAGCACACAAAAATTTGAAGCGTGCTGAGAAAGCCCGGATTTTGGTTGGAAAAATACCATCTATCATTGAAAGTCTGACTGCCAGAGTTAAAGCATGGGAGAGCGAGAAAGGAGTCCCTTTCTTATACGACAAGGTTCCCCTTTTACATATCGTGGAGGAGTACGCTGCACAACGGCAGGAGAGAGAGGAGGAAAAACGTAAATCTCGGGAGCAAAAACGTCTGCAAGAACAACATGCGACAGAACAGGAGGCACTATTTGGTTCAAGGCCCTCTACAAAGAAGCCATTGGGACAAAGCACCAATGCCAATACCATGGTTGGGACCCCAATTAGTCGTCGTACAAGTACCCCTTCGGGCCATCATGGAGTGTCAGCTGCAAAAGAACGCAGAGAAAGTGGGAGGGCCAACATAACACCTGTTAACTATGTTGCTCTTCCAAAGGATGATTCTGTTGCTCGGAGAAATTGA

Protein Analysis

567

Amino Acids

63.98

Weight (kDa)

6.64

Isoelectric Point (pI)

51.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 17 - 507 7.6e-75 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38720
fragaria_vesca FvH4_2g22412 FvH4_7g04100 FvH4_7g04100
malus_domestica MD02G1275300.v1.1 MD07G1043200.v1.1
prunus_persica Prupe.2G045700_v2.0.a1
pyrus_communis pycom07g03050
rosa_chinensis RchiOBHm_Chr1g0327971
rosa_laevigata RLG00000029953
rosa_multiflora Rmu_sc0000675.1_g000047 Rmu_ssc0000467.1_g000036
rosa_roxburghii Rroxscaffold_4G00322110
rosa_rugosa Rorug01G0072700
rosa_samantha Rh1AG088700 Rh1BG071200 Rh1CG086800 Rh1DG093400
rosa_wichuraiana Rw1G006890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 50
Acc36I ACCTGC 1 cut(s) 50
AccB1I GGYRCC 1 cut(s) 10
AccBSI CCGCTC 3 cut(s) 245, 647, 913
AccII CGCG 2 cut(s) 692, 826
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 4 cut(s) 809, 981, 1163, 1246
AcuI CTGAAG 2 cut(s) 728, 924
AfaI GTAC 4 cut(s) 182, 1397, 1576, 1582
AfiI CCNNNNNNNGG 2 cut(s) 1276, 1554
AflII CTTAAG 1 cut(s) 488
AgsI TTSAA 8 cut(s) 337, 374, 919, 928, 1083, 1252, 1304, 1500
AjnI CCWGG 1 cut(s) 417
AjuI GAANNNNNNNTTGG 4 cut(s) 302, 334, 1666, 1698
Alw21I GWGCWC 2 cut(s) 975, 1240
Alw26I GTCTC 1 cut(s) 779
Ama87I CYCGRG 2 cut(s) 128, 1440
AoxI GGCC 5 cut(s) 214, 415, 1502, 1591, 1638
ApeKI GCWGC 3 cut(s) 1038, 1400, 1610
ApoI RAATTY 4 cut(s) 809, 981, 1163, 1246
AspLEI GCGC 3 cut(s) 47, 694, 828
AspS9I GGNCC 6 cut(s) 282, 292, 1503, 1557, 1591, 1638
AsuC2I CCSGG 2 cut(s) 409, 1270
AsuHPI GGTGA 2 cut(s) 364, 446
AvaI CYCGRG 2 cut(s) 128, 1440
AvaII GGWCC 3 cut(s) 282, 292, 1557
AxyI CCTNAGG 1 cut(s) 287
BaeGI GKGCMC 1 cut(s) 277
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 1 cut(s) 975
BauI CACGAG 1 cut(s) 276
Bbv12I GWGCWC 2 cut(s) 975, 1240
BbvI GCAGC 3 cut(s) 1050, 1387, 1597
BccI CCATC 5 cut(s) 242, 569, 1010, 1300, 1602
BceAI ACGGC 4 cut(s) 229, 290, 430, 1424
BciT130I CCWGG 1 cut(s) 419
BclI TGATCA 2 cut(s) 634, 1093
BcnI CCSGG 2 cut(s) 409, 1270
BcoDI GTCTC 1 cut(s) 779
BfaI CTAG 3 cut(s) 681, 878, 1023
BfrI CTTAAG 1 cut(s) 488
BfuAI ACCTGC 1 cut(s) 50
BisI GCNGC 7 cut(s) 14, 17, 231, 645, 1039, 1401, 1611
BlsI GCNGC 7 cut(s) 15, 18, 232, 646, 1040, 1402, 1612
Bme1390I CCNGG 3 cut(s) 409, 419, 1270
Bme18I GGWCC 3 cut(s) 282, 292, 1557
BmeT110I CYCGRG 2 cut(s) 128, 1440
BmgT120I GGNCC 6 cut(s) 282, 292, 1503, 1557, 1591, 1638
BmiI GGNNCC 7 cut(s) 12, 283, 284, 293, 1371, 1558, 1559
BmrFI CCNGG 3 cut(s) 409, 419, 1270
BmsI GCATC 3 cut(s) 430, 669, 857
BplI GAGNNNNNCTC 2 cut(s) 270, 302
Bpu10I CCTNAGC 1 cut(s) 205
BpuEI CTTGAG 1 cut(s) 649
BpuMI CCSGG 2 cut(s) 409, 1270
Bsa29I ATCGAT 1 cut(s) 1216
BsaBI GATNNNNATC 1 cut(s) 1212
BsaJI CCNNGG 2 cut(s) 269, 1548
BsaXI ACNNNNNCTCC 2 cut(s) 1169, 1199
Bsc4I CCNNNNNNNGG 2 cut(s) 1276, 1554
Bse118I RCCGGY 1 cut(s) 431
Bse21I CCTNAGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 673
Bse8I GATNNNNATC 1 cut(s) 1212
BseBI CCWGG 1 cut(s) 419
BseCI ATCGAT 1 cut(s) 1216
BseDI CCNNGG 2 cut(s) 269, 1548
BseGI GGATG 7 cut(s) 445, 561, 872, 1009, 1096, 1213, 1684
BseJI GATNNNNATC 1 cut(s) 1212
BseLI CCNNNNNNNGG 2 cut(s) 1276, 1554
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 8 cut(s) 301, 320, 588, 786, 855, 888, 1048, 1251
BseRI GAGGAG 6 cut(s) 44, 1005, 1190, 1248, 1406, 1436
BseSI GKGCMC 1 cut(s) 277
BseXI GCAGC 3 cut(s) 1050, 1387, 1597
BsgI GTGCAG 2 cut(s) 1245, 1386
Bsh1236I CGCG 2 cut(s) 692, 826
BshFI GGCC 5 cut(s) 216, 417, 1504, 1593, 1640
BshNI GGYRCC 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 1216
BsiHKAI GWGCWC 2 cut(s) 975, 1240
BsiHKCI CYCGRG 2 cut(s) 128, 1440
BsiSI CCGG 4 cut(s) 408, 432, 444, 1270
BslFI GGGAC 9 cut(s) 92, 122, 295, 305, 371, 478, 1334, 1539, 1570
BslI CCNNNNNNNGG 2 cut(s) 1276, 1554
BsmAI GTCTC 1 cut(s) 779
BsmFI GGGAC 9 cut(s) 92, 122, 295, 305, 371, 478, 1334, 1539, 1570
BsmI GAATGC 1 cut(s) 433
BsnI GGCC 5 cut(s) 216, 417, 1504, 1593, 1640
BsoBI CYCGRG 2 cut(s) 128, 1440
Bsp1286I GDGCHC 3 cut(s) 277, 975, 1240
Bsp143I GATC 4 cut(s) 69, 634, 1072, 1093
Bsp19I CCATGG 1 cut(s) 1548
BspANI GGCC 5 cut(s) 216, 417, 1504, 1593, 1640
BspCNI CTCAG 8 cut(s) 300, 321, 587, 787, 854, 889, 1047, 1252
BspDI ATCGAT 1 cut(s) 1216
BspFNI CGCG 2 cut(s) 692, 826
BspLI GGNNCC 7 cut(s) 12, 283, 284, 293, 1371, 1558, 1559
BspMI ACCTGC 1 cut(s) 50
BspQI GCTCTTC 1 cut(s) 1674
BspT107I GGYRCC 1 cut(s) 10
BspTI CTTAAG 1 cut(s) 488
BsrBI CCGCTC 3 cut(s) 245, 647, 913
BsrDI GCAATG 1 cut(s) 673
BsrFI RCCGGY 1 cut(s) 431
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 269, 1548
BssMI GATC 4 cut(s) 69, 634, 1072, 1093
BssSI CACGAG 1 cut(s) 276
BssT1I CCWWGG 2 cut(s) 269, 1548
Bst2BI CACGAG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 2 cut(s) 101, 469
Bst6I CTCTTC 3 cut(s) 467, 1225, 1674
BstAFI CTTAAG 1 cut(s) 488
BstAPI GCANNNNNTGC 2 cut(s) 42, 730
BstC8I GCNNGC 4 cut(s) 758, 934, 1119, 1257
BstDSI CCRYGG 1 cut(s) 1548
BstF5I GGATG 7 cut(s) 445, 561, 872, 1009, 1096, 1213, 1684
BstFNI CGCG 2 cut(s) 692, 826
BstHHI GCGC 3 cut(s) 47, 694, 828
BstKTI GATC 4 cut(s) 72, 637, 1075, 1096
BstMAI GTCTC 1 cut(s) 779
BstMBI GATC 4 cut(s) 69, 634, 1072, 1093
BstMWI GCNNNNNNNGC 9 cut(s) 42, 227, 236, 428, 730, 745, 859, 970, 1265
BstNI CCWGG 1 cut(s) 419
BstNSI RCATGY 1 cut(s) 1472
BstSCI CCNGG 3 cut(s) 407, 417, 1268
BstSLI GKGCMC 1 cut(s) 277
BstUI CGCG 2 cut(s) 692, 826
BstV1I GCAGC 3 cut(s) 1050, 1387, 1597
BstXI CCANNNNNNTGG 1 cut(s) 1549
Bsu15I ATCGAT 1 cut(s) 1216
Bsu36I CCTNAGG 1 cut(s) 287
BsuRI GGCC 5 cut(s) 216, 417, 1504, 1593, 1640
BsuTUI ATCGAT 1 cut(s) 1216
BtgI CCRYGG 1 cut(s) 1548
BtgZI GCGATG 1 cut(s) 220
BtsCI GGATG 7 cut(s) 445, 561, 872, 1009, 1096, 1213, 1684
BtsIMutI CAGTG 1 cut(s) 106
BveI ACCTGC 1 cut(s) 50
Cac8I GCNNGC 4 cut(s) 758, 934, 1119, 1257
CfoI GCGC 3 cut(s) 47, 694, 828
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 6 cut(s) 282, 292, 1503, 1557, 1591, 1638
ClaI ATCGAT 1 cut(s) 1216
Csp6I GTAC 4 cut(s) 181, 1396, 1575, 1581
CviQI GTAC 4 cut(s) 181, 1396, 1575, 1581
DpnI GATC 4 cut(s) 71, 636, 1074, 1095
DpnII GATC 4 cut(s) 69, 634, 1072, 1093
EaeI YGGCCR 1 cut(s) 214
Eam1104I CTCTTC 3 cut(s) 467, 1225, 1674
EarI CTCTTC 3 cut(s) 467, 1225, 1674
EciI GGCGGA 2 cut(s) 292, 631
Ecl136II GAGCTC 1 cut(s) 973
Eco130I CCWWGG 2 cut(s) 269, 1548
Eco24I GRGCYC 1 cut(s) 975
Eco32I GATATC 1 cut(s) 300
Eco47I GGWCC 3 cut(s) 282, 292, 1557
Eco53kI GAGCTC 1 cut(s) 973
Eco57I CTGAAG 2 cut(s) 728, 924
Eco81I CCTNAGG 1 cut(s) 287
Eco88I CYCGRG 2 cut(s) 128, 1440
EcoICRI GAGCTC 1 cut(s) 973
EcoO109I RGGNCCY 3 cut(s) 282, 1503, 1557
EcoRII CCWGG 1 cut(s) 417
EcoRV GATATC 1 cut(s) 300
EcoT14I CCWWGG 2 cut(s) 269, 1548
EcoT38I GRGCYC 1 cut(s) 975
ErhI CCWWGG 2 cut(s) 269, 1548
FalI AAGNNNNNCTT 2 cut(s) 1570, 1602
FaqI GGGAC 9 cut(s) 92, 122, 295, 305, 371, 478, 1334, 1539, 1570
FauI CCCGC 1 cut(s) 41
FbaI TGATCA 2 cut(s) 634, 1093
Fnu4HI GCNGC 7 cut(s) 14, 17, 231, 645, 1039, 1401, 1611
FokI GGATG 7 cut(s) 452, 548, 879, 1016, 1103, 1220, 1691
FriOI GRGCYC 1 cut(s) 975
Fsp4HI GCNGC 7 cut(s) 14, 17, 231, 645, 1039, 1401, 1611
FspBI CTAG 3 cut(s) 681, 878, 1023
GlaI GCGC 3 cut(s) 46, 693, 827
GluI GCNGC 7 cut(s) 14, 17, 231, 645, 1039, 1401, 1611
HaeIII GGCC 5 cut(s) 216, 417, 1504, 1593, 1640
HapII CCGG 4 cut(s) 408, 432, 444, 1270
HhaI GCGC 3 cut(s) 47, 694, 828
Hin6I GCGC 3 cut(s) 45, 692, 826
HinP1I GCGC 3 cut(s) 45, 692, 826
HincII GTYRAC 1 cut(s) 1657
HindII GTYRAC 1 cut(s) 1657
HindIII AAGCTT 2 cut(s) 625, 1119
HinfI GANTC 7 cut(s) 447, 775, 993, 1030, 1049, 1347, 1682
HpaI GTTAAC 1 cut(s) 1657
HpaII CCGG 4 cut(s) 408, 432, 444, 1270
HphI GGTGA 2 cut(s) 364, 446
Hpy166II GTNNAC 4 cut(s) 533, 619, 997, 1657
Hpy188I TCNGA 7 cut(s) 156, 251, 400, 1072, 1098, 1311, 1695
Hpy188III TCNNGA 2 cut(s) 878, 1442
Hpy8I GTNNAC 4 cut(s) 533, 619, 997, 1657
Hpy99I CGWCG 1 cut(s) 1575
HpyAV CCTTC 4 cut(s) 154, 532, 937, 1596
HpyCH4III ACNGT 2 cut(s) 101, 469
HpyCH4IV ACGT 3 cut(s) 529, 1432, 1453
HpyCH4V TGCA 8 cut(s) 196, 733, 1161, 1169, 1226, 1403, 1459, 1613
HpyF10VI GCNNNNNNNGC 9 cut(s) 42, 227, 236, 428, 730, 745, 859, 970, 1265
HpySE526I ACGT 3 cut(s) 529, 1432, 1453
HspAI GCGC 3 cut(s) 45, 692, 826
KflI GGGWCCC 2 cut(s) 282, 1557
Ksp22I TGATCA 2 cut(s) 634, 1093
KspAI GTTAAC 1 cut(s) 1657
Kzo9I GATC 4 cut(s) 69, 634, 1072, 1093
LguI GCTCTTC 1 cut(s) 1674
LmnI GCTCC 3 cut(s) 1019, 1235, 1444
Lsp1109I GCAGC 3 cut(s) 1050, 1387, 1597
LweI GCATC 3 cut(s) 430, 669, 857
MaeI CTAG 3 cut(s) 681, 878, 1023
MaeII ACGT 3 cut(s) 529, 1432, 1453
MaeIII GTNAC 2 cut(s) 95, 820
MalI GATC 4 cut(s) 71, 636, 1074, 1095
MbiI CCGCTC 3 cut(s) 245, 647, 913
MboI GATC 4 cut(s) 69, 634, 1072, 1093
MboII GAAGA 8 cut(s) 169, 349, 484, 623, 828, 989, 1242, 1661
MhlI GDGCHC 3 cut(s) 277, 975, 1240
MlyI GAGTC 3 cut(s) 456, 1002, 1356
MmeI TCCRAC 2 cut(s) 148, 1261
MseI TTAA 7 cut(s) 489, 830, 957, 1062, 1124, 1323, 1656
MslI CAYNNNNRTG 1 cut(s) 1002
MspA1I CMGCKG 1 cut(s) 1610
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 4 cut(s) 408, 432, 444, 1270
MspR9I CCNGG 3 cut(s) 409, 419, 1270
Mva1269I GAATGC 1 cut(s) 433
MvaI CCWGG 1 cut(s) 419
MvnI CGCG 2 cut(s) 692, 826
MwoI GCNNNNNNNGC 9 cut(s) 42, 227, 236, 428, 730, 745, 859, 970, 1265
NciI CCSGG 2 cut(s) 409, 1270
NcoI CCATGG 1 cut(s) 1548
NdeII GATC 4 cut(s) 69, 634, 1072, 1093
NlaIV GGNNCC 7 cut(s) 12, 283, 284, 293, 1371, 1558, 1559
NmuCI GTSAC 1 cut(s) 95
NspI RCATGY 1 cut(s) 1472
PaeR7I CTCGAG 1 cut(s) 128
PaqCI CACCTGC 1 cut(s) 50
PciSI GCTCTTC 1 cut(s) 1674
PctI GAATGC 1 cut(s) 433
PfeI GAWTC 4 cut(s) 775, 1030, 1049, 1682
PkrI GCNGC 7 cut(s) 15, 18, 232, 646, 1040, 1402, 1612
PleI GAGTC 3 cut(s) 455, 1001, 1355
PpsI GAGTC 3 cut(s) 455, 1001, 1355
PpuMI RGGWCCY 2 cut(s) 282, 1557
Psp124BI GAGCTC 1 cut(s) 975
Psp5II RGGWCCY 2 cut(s) 282, 1557
Psp6I CCWGG 1 cut(s) 417
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 7 cut(s) 12, 283, 284, 293, 1371, 1558, 1559
PspPI GGNCC 6 cut(s) 282, 292, 1503, 1557, 1591, 1638
PspPPI RGGWCCY 2 cut(s) 282, 1557
PspXI VCTCGAGB 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 1610
RsaI GTAC 4 cut(s) 182, 1397, 1576, 1582
RsaNI GTAC 4 cut(s) 181, 1396, 1575, 1581
RseI CAYNNNNRTG 1 cut(s) 1002
SacI GAGCTC 1 cut(s) 975
SapI GCTCTTC 1 cut(s) 1674
SaqAI TTAA 7 cut(s) 489, 830, 957, 1062, 1124, 1323, 1656
SatI GCNGC 7 cut(s) 14, 17, 231, 645, 1039, 1401, 1611
Sau3AI GATC 4 cut(s) 69, 634, 1072, 1093
Sau96I GGNCC 6 cut(s) 282, 292, 1503, 1557, 1591, 1638
SchI GAGTC 3 cut(s) 456, 1002, 1356
ScrFI CCNGG 3 cut(s) 409, 419, 1270
SduI GDGCHC 3 cut(s) 277, 975, 1240
SfaNI GCATC 3 cut(s) 430, 669, 857
Sfr274I CTCGAG 1 cut(s) 128
SinI GGWCC 3 cut(s) 282, 292, 1557
SlaI CTCGAG 1 cut(s) 128
SmiMI CAYNNNNRTG 1 cut(s) 1002
SmlI CTYRAG 3 cut(s) 128, 488, 628
SmoI CTYRAG 3 cut(s) 128, 488, 628
SspMI CTAG 3 cut(s) 681, 878, 1023
SstI GAGCTC 1 cut(s) 975
StyD4I CCNGG 3 cut(s) 407, 417, 1268
StyI CCWWGG 2 cut(s) 269, 1548
TaaI ACNGT 2 cut(s) 101, 469
TaiI ACGT 3 cut(s) 532, 1435, 1456
TaqI TCGA 7 cut(s) 129, 144, 450, 609, 909, 975, 1216
TatI WGTACW 1 cut(s) 180
TauI GCSGC 4 cut(s) 16, 19, 233, 647
TfiI GAWTC 4 cut(s) 775, 1030, 1049, 1682
Tru1I TTAA 7 cut(s) 489, 830, 957, 1062, 1124, 1323, 1656
Tru9I TTAA 7 cut(s) 489, 830, 957, 1062, 1124, 1323, 1656
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 3 cut(s) 1038, 1400, 1610
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 4 cut(s) 75, 956, 1215, 1224
TspGWI ACGGA 1 cut(s) 817
TspRI CASTG 1 cut(s) 106
Vha464I CTTAAG 1 cut(s) 488
VpaK11BI GGWCC 3 cut(s) 282, 292, 1557
XapI RAATTY 4 cut(s) 809, 981, 1163, 1246
XbaI TCTAGA 1 cut(s) 877
XceI RCATGY 1 cut(s) 1472
XhoI CTCGAG 1 cut(s) 128
XspI CTAG 3 cut(s) 681, 878, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.