Rh1CG086800

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0012187
Physical Location & Seq
Reverse (-)
18182531 .. 18188919
6389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG086800.1

Sequence Viewer

Length: 1329 bp
ATGTCGGCGGTGCCGCCGCCCGTTTCTCCCTCCCGCACCACCTGCGCTTCTCTCCTCCATGAACTACAGATCATTTGGGACGAAATTGGGGAGAGTGACAGTGAGAGGGACACCATGCTTCTCCAACTCGAGCAAGAATGCCTCGACATCTATCGGAAGAAGGTGGAAATGACGAGAAAGTACAGGGCTGATTTGCACAAGTCCTTAGCCGACGGCCAAGCTCAAATCGCCGCCATCGCCTCCGCTCTCGGAGAACCTAATTCGTTTCCAAGGGCAAGAGGGACCCTCAGGGACCAGATATCCTCCGTGAAGCCTGTTTTGGACGAACTGAGCTTGAAGAGGCAGGAAAGGGTGAGGGACTTTTCGGTAATTCAAACACAGATTGCGAGGATTTCGTCTGAAATAGCCGGGAACGGCCTGGCTAAGAATGCCGGAGTGGAGGAGCGTGATTTGACTGTGAAGAGGTTGGGAGAGCTTAAGTCCCACCTTGACGAACTTCACAAAGAAAAGGCTTTACGTTTACAGAAGGTCAATAGTCATGTCAATACCATCCATGACCTCTCAGTTGTAATGTCCATTGATTTCTTTAGGACACTCGAAGAAGTCCACCCAAGCTTGAGTGATCAGTCCGCCGCTCATTCCAAGAGCATCAGCAATGACACTCTTGCTAGATTGACCGCACTGACCAACTCGCTGAAGCAAGAGAAACAGCAGAGACTGCAAAAGCTACAAGAGCTTGGAAGCAAGCTGATAGAAGTATGGAATCTCATGGAGACACCAACTGAGGAACGGAACAAATTTGACCATGTTACACGCTTAATTTCTTCCTCTGTTGATGAGCTGTCAAGGCATGGATGCCTTTCTCTAGATGTCATTGAGCAGACTGAGGCTGAAGTCGAGCGGTTGAATGTTTTGAAAGCCAGCAAGATGAAGGAGTTGGTCTTTAAGAGGCAAAATGAGCTCGAAGAAATTTATAGAGGAGTCCACATGGATGTAGATAGTGATGGAGCTAGACAGATTCTCAGCAGCCTCATAGATTCTGGTAATGTTAATTTGTCTGATCTGCTTTCAAGCATGGATGATCAGATTGTAAAGGCAAAAGAGCAAGCTTTAAGCAGAAAGGATATTTTGGATAAGGTAGAGAAATGCAAATTTGCATTTGAGGAGGATAAGTGGCTTGATGAGTATGAAAAGGATGAAAATCGATATAGTGCAGGAAGAGGAGCACACAAAAATTTGAAGCGTGCTGAGAAAGCCCGGATTTTGGTTGCAAAAATACCATGTAAGCTTGTAATCATCCATATGACTAATTCTTATCTAGTTATATAA

Protein Analysis

442

Amino Acids

50.11

Weight (kDa)

6.17

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 17 - 427 3.1e-56 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38720
fragaria_vesca FvH4_2g22412 FvH4_7g04100 FvH4_7g04100
malus_domestica MD02G1275300.v1.1 MD07G1043200.v1.1
prunus_persica Prupe.2G045700_v2.0.a1
pyrus_communis pycom07g03050
rosa_chinensis RchiOBHm_Chr1g0327971
rosa_laevigata RLG00000029953
rosa_multiflora Rmu_sc0000675.1_g000047 Rmu_ssc0000467.1_g000036
rosa_roxburghii Rroxscaffold_4G00322110
rosa_rugosa Rorug01G0072700
rosa_samantha Rh1AG088700 Rh1BG071200 Rh1CG086800 Rh1DG093400
rosa_wichuraiana Rw1G006890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 50
Acc36I ACCTGC 1 cut(s) 50
AccB1I GGYRCC 1 cut(s) 10
AccBSI CCGCTC 3 cut(s) 245, 635, 901
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 4 cut(s) 797, 969, 1151, 1234
AcuI CTGAAG 2 cut(s) 716, 912
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 1 cut(s) 1264
AflII CTTAAG 1 cut(s) 476
AgsI TTSAA 6 cut(s) 337, 374, 907, 916, 1071, 1240
AjnI CCWGG 1 cut(s) 417
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
Alw21I GWGCWC 2 cut(s) 963, 1228
Alw26I GTCTC 2 cut(s) 709, 767
AlwNI CAGNNNCTG 1 cut(s) 718
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 2 cut(s) 214, 415
ApeKI GCWGC 1 cut(s) 1026
ApoI RAATTY 4 cut(s) 797, 969, 1151, 1234
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 2 cut(s) 282, 292
AsuC2I CCSGG 2 cut(s) 409, 1258
AsuHPI GGTGA 1 cut(s) 364
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 2 cut(s) 282, 292
AxyI CCTNAGG 1 cut(s) 287
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 1 cut(s) 963
Bbv12I GWGCWC 2 cut(s) 963, 1228
BbvI GCAGC 1 cut(s) 1038
BccI CCATC 3 cut(s) 242, 557, 998
BceAI ACGGC 2 cut(s) 229, 430
BciT130I CCWGG 1 cut(s) 419
BclI TGATCA 2 cut(s) 622, 1081
BcnI CCSGG 2 cut(s) 409, 1258
BcoDI GTCTC 2 cut(s) 709, 767
BfaI CTAG 4 cut(s) 669, 866, 1011, 1319
BfmI CTRYAG 1 cut(s) 65
BfrI CTTAAG 1 cut(s) 476
BfuAI ACCTGC 1 cut(s) 50
BisI GCNGC 5 cut(s) 14, 17, 231, 633, 1027
BlsI GCNGC 5 cut(s) 15, 18, 232, 634, 1028
Bme1390I CCNGG 3 cut(s) 409, 419, 1258
Bme18I GGWCC 2 cut(s) 282, 292
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 282, 292
BmiI GGNNCC 4 cut(s) 12, 283, 284, 293
BmrFI CCNGG 3 cut(s) 409, 419, 1258
BmsI GCATC 2 cut(s) 657, 845
BplI GAGNNNNNCTC 2 cut(s) 270, 302
Bpu10I CCTNAGC 1 cut(s) 205
BpuEI CTTGAG 1 cut(s) 637
BpuMI CCSGG 2 cut(s) 409, 1258
Bsa29I ATCGAT 1 cut(s) 1204
BsaBI GATNNNNATC 1 cut(s) 1200
BsaJI CCNNGG 1 cut(s) 269
BsaXI ACNNNNNCTCC 2 cut(s) 1157, 1187
Bsc4I CCNNNNNNNGG 1 cut(s) 1264
Bse21I CCTNAGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 661
Bse8I GATNNNNATC 1 cut(s) 1200
BseBI CCWGG 1 cut(s) 419
BseCI ATCGAT 1 cut(s) 1204
BseDI CCNNGG 1 cut(s) 269
BseGI GGATG 6 cut(s) 549, 860, 997, 1084, 1201, 1296
BseJI GATNNNNATC 1 cut(s) 1200
BseLI CCNNNNNNNGG 1 cut(s) 1264
BseMI GCAATG 1 cut(s) 661
BseMII CTCAG 7 cut(s) 301, 320, 576, 774, 876, 1036, 1239
BseRI GAGGAG 5 cut(s) 44, 455, 993, 1178, 1236
BseXI GCAGC 1 cut(s) 1038
BsgI GTGCAG 1 cut(s) 1233
BshFI GGCC 2 cut(s) 216, 417
BshNI GGYRCC 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 1204
BsiHKAI GWGCWC 2 cut(s) 963, 1228
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 3 cut(s) 408, 432, 1258
BslFI GGGAC 6 cut(s) 92, 122, 295, 305, 371, 466
BslI CCNNNNNNNGG 1 cut(s) 1264
BsmAI GTCTC 2 cut(s) 709, 767
BsmFI GGGAC 6 cut(s) 92, 122, 295, 305, 371, 466
BsmI GAATGC 2 cut(s) 143, 433
BsnI GGCC 2 cut(s) 216, 417
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 963, 1228
Bsp143I GATC 4 cut(s) 69, 622, 1060, 1081
BspANI GGCC 2 cut(s) 216, 417
BspCNI CTCAG 7 cut(s) 300, 321, 575, 775, 877, 1035, 1240
BspDI ATCGAT 1 cut(s) 1204
BspLI GGNNCC 4 cut(s) 12, 283, 284, 293
BspMI ACCTGC 1 cut(s) 50
BspT107I GGYRCC 1 cut(s) 10
BspTI CTTAAG 1 cut(s) 476
BsrBI CCGCTC 3 cut(s) 245, 635, 901
BsrDI GCAATG 1 cut(s) 661
BssECI CCNNGG 1 cut(s) 269
BssMI GATC 4 cut(s) 69, 622, 1060, 1081
BssT1I CCWWGG 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 2 cut(s) 101, 457
Bst6I CTCTTC 3 cut(s) 332, 455, 1213
BstAFI CTTAAG 1 cut(s) 476
BstAPI GCANNNNNTGC 2 cut(s) 42, 718
BstC8I GCNNGC 4 cut(s) 746, 922, 1107, 1245
BstDEI CTNAG 9 cut(s) 205, 287, 329, 423, 562, 783, 885, 1022, 1248
BstF5I GGATG 6 cut(s) 549, 860, 997, 1084, 1201, 1296
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 4 cut(s) 72, 625, 1063, 1084
BstMAI GTCTC 2 cut(s) 709, 767
BstMBI GATC 4 cut(s) 69, 622, 1060, 1081
BstMWI GCNNNNNNNGC 9 cut(s) 42, 227, 236, 428, 718, 733, 847, 958, 1253
BstNI CCWGG 1 cut(s) 419
BstSCI CCNGG 3 cut(s) 407, 417, 1256
BstSFI CTRYAG 1 cut(s) 65
BstV1I GCAGC 1 cut(s) 1038
Bsu15I ATCGAT 1 cut(s) 1204
Bsu36I CCTNAGG 1 cut(s) 287
BsuRI GGCC 2 cut(s) 216, 417
BsuTUI ATCGAT 1 cut(s) 1204
BtgZI GCGATG 1 cut(s) 220
BtsCI GGATG 6 cut(s) 549, 860, 997, 1084, 1201, 1296
BtsIMutI CAGTG 2 cut(s) 106, 680
BveI ACCTGC 1 cut(s) 50
Cac8I GCNNGC 4 cut(s) 746, 922, 1107, 1245
CaiI CAGNNNCTG 1 cut(s) 718
CfoI GCGC 1 cut(s) 47
Cfr13I GGNCC 2 cut(s) 282, 292
ClaI ATCGAT 1 cut(s) 1204
Csp6I GTAC 1 cut(s) 181
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 9 cut(s) 205, 287, 329, 423, 562, 783, 885, 1022, 1248
DpnI GATC 4 cut(s) 71, 624, 1062, 1083
DpnII GATC 4 cut(s) 69, 622, 1060, 1081
EaeI YGGCCR 1 cut(s) 214
Eam1104I CTCTTC 3 cut(s) 332, 455, 1213
EarI CTCTTC 3 cut(s) 332, 455, 1213
EciI GGCGGA 1 cut(s) 619
Ecl136II GAGCTC 1 cut(s) 961
Eco130I CCWWGG 1 cut(s) 269
Eco24I GRGCYC 1 cut(s) 963
Eco32I GATATC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 282, 292
Eco53kI GAGCTC 1 cut(s) 961
Eco57I CTGAAG 2 cut(s) 716, 912
Eco81I CCTNAGG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 128
EcoICRI GAGCTC 1 cut(s) 961
EcoO109I RGGNCCY 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 417
EcoRV GATATC 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 963
ErhI CCWWGG 1 cut(s) 269
FaqI GGGAC 6 cut(s) 92, 122, 295, 305, 371, 466
FauI CCCGC 1 cut(s) 41
FauNDI CATATG 1 cut(s) 1302
FbaI TGATCA 2 cut(s) 622, 1081
Fnu4HI GCNGC 5 cut(s) 14, 17, 231, 633, 1027
FokI GGATG 6 cut(s) 536, 867, 1004, 1091, 1208, 1283
FriOI GRGCYC 1 cut(s) 963
Fsp4HI GCNGC 5 cut(s) 14, 17, 231, 633, 1027
FspBI CTAG 4 cut(s) 669, 866, 1011, 1319
GlaI GCGC 1 cut(s) 46
GluI GCNGC 5 cut(s) 14, 17, 231, 633, 1027
HaeIII GGCC 2 cut(s) 216, 417
HapII CCGG 3 cut(s) 408, 432, 1258
HhaI GCGC 1 cut(s) 47
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HindIII AAGCTT 3 cut(s) 613, 1107, 1286
HinfI GANTC 4 cut(s) 763, 981, 1018, 1037
HpaII CCGG 3 cut(s) 408, 432, 1258
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 3 cut(s) 521, 607, 985
Hpy188I TCNGA 5 cut(s) 156, 251, 400, 1060, 1086
Hpy188III TCNNGA 1 cut(s) 866
Hpy8I GTNNAC 3 cut(s) 521, 607, 985
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 3 cut(s) 154, 520, 925
HpyCH4III ACNGT 2 cut(s) 101, 457
HpyCH4IV ACGT 1 cut(s) 517
HpyCH4V TGCA 6 cut(s) 196, 721, 1149, 1157, 1214, 1271
HpyF10VI GCNNNNNNNGC 9 cut(s) 42, 227, 236, 428, 718, 733, 847, 958, 1253
HpyF3I CTNAG 9 cut(s) 205, 287, 329, 423, 562, 783, 885, 1022, 1248
HpySE526I ACGT 1 cut(s) 517
HspAI GCGC 1 cut(s) 45
KflI GGGWCCC 1 cut(s) 282
Ksp22I TGATCA 2 cut(s) 622, 1081
Kzo9I GATC 4 cut(s) 69, 622, 1060, 1081
LmnI GCTCC 3 cut(s) 442, 1007, 1223
Lsp1109I GCAGC 1 cut(s) 1038
LweI GCATC 2 cut(s) 657, 845
MaeI CTAG 4 cut(s) 669, 866, 1011, 1319
MaeII ACGT 1 cut(s) 517
MaeIII GTNAC 2 cut(s) 95, 808
MalI GATC 4 cut(s) 71, 624, 1062, 1083
MbiI CCGCTC 3 cut(s) 245, 635, 901
MboI GATC 4 cut(s) 69, 622, 1060, 1081
MboII GAAGA 7 cut(s) 169, 349, 472, 611, 816, 977, 1230
MhlI GDGCHC 2 cut(s) 963, 1228
MlyI GAGTC 1 cut(s) 990
MmeI TCCRAC 1 cut(s) 148
MseI TTAA 5 cut(s) 477, 818, 945, 1050, 1112
MslI CAYNNNNRTG 2 cut(s) 990, 1301
MspCI CTTAAG 1 cut(s) 476
MspI CCGG 3 cut(s) 408, 432, 1258
MspR9I CCNGG 3 cut(s) 409, 419, 1258
Mva1269I GAATGC 2 cut(s) 143, 433
MvaI CCWGG 1 cut(s) 419
MwoI GCNNNNNNNGC 9 cut(s) 42, 227, 236, 428, 718, 733, 847, 958, 1253
NciI CCSGG 2 cut(s) 409, 1258
NdeI CATATG 1 cut(s) 1302
NdeII GATC 4 cut(s) 69, 622, 1060, 1081
NlaIV GGNNCC 4 cut(s) 12, 283, 284, 293
NmuCI GTSAC 1 cut(s) 95
PaeR7I CTCGAG 1 cut(s) 128
PaqCI CACCTGC 1 cut(s) 50
PctI GAATGC 2 cut(s) 143, 433
PfeI GAWTC 3 cut(s) 763, 1018, 1037
PkrI GCNGC 5 cut(s) 15, 18, 232, 634, 1028
PleI GAGTC 1 cut(s) 989
PpsI GAGTC 1 cut(s) 989
PpuMI RGGWCCY 1 cut(s) 282
Psp124BI GAGCTC 1 cut(s) 963
Psp5II RGGWCCY 1 cut(s) 282
Psp6I CCWGG 1 cut(s) 417
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 4 cut(s) 12, 283, 284, 293
PspPI GGNCC 2 cut(s) 282, 292
PspPPI RGGWCCY 1 cut(s) 282
PspXI VCTCGAGB 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 718
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
RseI CAYNNNNRTG 2 cut(s) 990, 1301
SacI GAGCTC 1 cut(s) 963
SaqAI TTAA 5 cut(s) 477, 818, 945, 1050, 1112
SatI GCNGC 5 cut(s) 14, 17, 231, 633, 1027
Sau3AI GATC 4 cut(s) 69, 622, 1060, 1081
Sau96I GGNCC 2 cut(s) 282, 292
SchI GAGTC 1 cut(s) 990
ScrFI CCNGG 3 cut(s) 409, 419, 1258
SduI GDGCHC 2 cut(s) 963, 1228
SfaNI GCATC 2 cut(s) 657, 845
SfcI CTRYAG 1 cut(s) 65
Sfr274I CTCGAG 1 cut(s) 128
SinI GGWCC 2 cut(s) 282, 292
SlaI CTCGAG 1 cut(s) 128
SmiMI CAYNNNNRTG 2 cut(s) 990, 1301
SmlI CTYRAG 3 cut(s) 128, 476, 616
SmoI CTYRAG 3 cut(s) 128, 476, 616
SspMI CTAG 4 cut(s) 669, 866, 1011, 1319
SstI GAGCTC 1 cut(s) 963
StyD4I CCNGG 3 cut(s) 407, 417, 1256
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 2 cut(s) 101, 457
TaiI ACGT 1 cut(s) 520
TaqI TCGA 6 cut(s) 129, 144, 597, 897, 963, 1204
TatI WGTACW 1 cut(s) 180
TauI GCSGC 4 cut(s) 16, 19, 233, 635
TfiI GAWTC 3 cut(s) 763, 1018, 1037
Tru1I TTAA 5 cut(s) 477, 818, 945, 1050, 1112
Tru9I TTAA 5 cut(s) 477, 818, 945, 1050, 1112
TscAI CASTG 2 cut(s) 106, 687
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 1026
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 4 cut(s) 75, 944, 1203, 1212
TspGWI ACGGA 2 cut(s) 295, 805
TspRI CASTG 2 cut(s) 106, 687
Vha464I CTTAAG 1 cut(s) 476
VpaK11BI GGWCC 2 cut(s) 282, 292
XapI RAATTY 4 cut(s) 797, 969, 1151, 1234
XbaI TCTAGA 1 cut(s) 865
XhoI CTCGAG 1 cut(s) 128
XspI CTAG 4 cut(s) 669, 866, 1011, 1319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.