pycom07g03050

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012187
Physical Location & Seq
Forward (+)
2503880 .. 2508501
4622 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g03050.2

Sequence Viewer

Length: 1698 bp
ATGACCACTAAGCCCTCGTCCGTCTCTCCCTCCCGCACCACCTGCGCTTCTCTCCTACGCGAATTGCAGATAATTTGGGATGAAATTGGGGAGAGTGACGATCAGAGGGACAAGATGCTTCTACAGCTTGAGCAAGAGTGTCTCGATATTTACCGGAGGAAGGTGGAAATGACCAGGAAGCACAGTGCCGAATTGCACCAGTACTTAGCGGACAGCAAAGCCCAGATCAAGGCCATTGCTTTGGCCCTTGGAGAAGTTGACTCCTTTTCTTGCGGAAGAGGGACTCTCAAAGATCAATTGTCTACCCTTAAACCTGTTCTGGACGAATTGATCTTGAAGAAGGAGAAAAGGGTCAAGGAGTTTTCAGCAGTTCTGTCCCAGATTGCAACGATTTCGGCTGAAATAGCCGGGGCTGGCGTGTCTGGGAATGTGGATGATCCAGGACTTGACGAGCGCGATTTGAGTGTGAAGAGGTTGCGGGACCTTCAGTCACAACTTGAGGAGCTTCAAAGAGAGAAGGTTTTGCGTCTAGAGAGAGTAAGTGCTAATGTAAACATCATCCATGAACTCTCGGTTGTAATGTCAATTGATTTCTGTAAGACAATCAACAAAGTTCACCCAAGCTTGACGGATCATTCCTCCGCTCAGTCCAAGAGCATCAGTAATGATACTCTTGCCAGATTGACGACTTTGACTGATTCATTAAAGCAAGAGAAACAACAAAGACTGCAAAAGCTAAAATATCTTGGGAGCAAACTGATAGAACTATGGAATCTGATTGAGACACCAATTGATGAACAGAAAAGATTTGACCATGTTACGAGCATAATTTCTTCCTCAGTTGATGAGGTGTCTAGGCAAGGATGCCTTTCCCTAGATGTCATTGAACAGACTGAGGTTGAAGTTGAGAGGTTGAATGTTTTGAAAGCCAGCAAGATGAAGGAGTTGGTATTCAAGAGGCAAAGTGAGCTGGAAGAAATTTACAGAGGGGTCCACATGGATGTAGATAGTGATTCAGCTAGAGAGATCCTCAGCAGTCTCATAGATTCTGGTAGTGTTAGTTTGTCTGATCTGCTTTCAAGCATGGATGATCAGATTCTAAGGGCCAAAGAGCAAGCTTTAAGCAGGAAGGATATTTTGGACAAGGTAGAGAAGTGCAAGTTTGCATCTGAGGAGGAAAAGTGGCTTGATGAATATGAAAAGGATGAAAATCGCTACAGCGCAGGAAGAGGAGCACACAAAAATTTAAAGCGTGCTGAGAAAGCCCGGATTCTGGCTGGAAAAATACCATCTATCATCGAAAATTTGACTGCCAAAGTTAAAGCCTGGGAGATTGAGAAAGGAATCCCTTTCTTATATGACAAGGTTCCCTTGTTACATACGTTGGAGGAGTACACTGCACAACGACAGGAGAGAGAGGAGGAGAAGCGTAAATCTCGGGAGCAAAAACGGCTTCAAGAACAACATAATGCAGAACAGGAGGCAATCTTTGGTTCAAGGCCTTCTACAAAGAAGCCATTGGGACAGAGCACAAGTGGTAACACCATGGTTGGCACACCAATCAATCGCCGCACACCTTTAGGCCGTCATGGAGTCTCAGCTGGAAAGGAACGCAAGGAAAGCGGAAGGGCACATAACGTAACGCCCATTAACTACGTTGCTCTTCCCAAGGATGATTCAGTATCTCGGAGAAACTGA

Protein Analysis

566

Amino Acids

63.96

Weight (kDa)

6.9

Isoelectric Point (pI)

54.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38720
fragaria_vesca FvH4_2g22412 FvH4_7g04100 FvH4_7g04100
malus_domestica MD02G1275300.v1.1 MD07G1043200.v1.1
prunus_persica Prupe.2G045700_v2.0.a1
pyrus_communis pycom07g03050
rosa_chinensis RchiOBHm_Chr1g0327971
rosa_laevigata RLG00000029953
rosa_multiflora Rmu_sc0000675.1_g000047 Rmu_ssc0000467.1_g000036
rosa_roxburghii Rroxscaffold_4G00322110
rosa_rugosa Rorug01G0072700
rosa_samantha Rh1AG088700 Rh1BG071200 Rh1CG086800 Rh1DG093400
rosa_wichuraiana Rw1G006890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 50
Acc36I ACCTGC 1 cut(s) 50
AccBSI CCGCTC 1 cut(s) 644
AccI GTMKAC 1 cut(s) 302
AccII CGCG 2 cut(s) 60, 456
AciI CCGC 7 cut(s) 34, 209, 273, 478, 642, 1570, 1623
AclWI GGATC 3 cut(s) 431, 639, 1021
AcsI RAATTY 3 cut(s) 978, 1243, 1303
AcuI CTGAAG 1 cut(s) 470
AfaI GTAC 2 cut(s) 203, 1394
AfiI CCNNNNNNNGG 3 cut(s) 160, 229, 1273
AhdI GACNNNNNGTC 1 cut(s) 487
AjnI CCWGG 3 cut(s) 173, 439, 1325
AjuI GAANNNNNNNTTGG 2 cut(s) 1121, 1153
AluBI AGCT 8 cut(s) 127, 505, 624, 736, 970, 1019, 1118, 1601
AluI AGCT 8 cut(s) 127, 505, 624, 736, 970, 1019, 1118, 1601
Alw21I GWGCWC 2 cut(s) 1237, 1532
Alw26I GTCTC 5 cut(s) 28, 146, 776, 1043, 1600
AlwI GGATC 3 cut(s) 431, 639, 1021
Ama87I CYCGRG 1 cut(s) 1437
AoxI GGCC 5 cut(s) 231, 243, 1104, 1499, 1582
ApoI RAATTY 3 cut(s) 978, 1243, 1303
AspLEI GCGC 3 cut(s) 47, 456, 1223
AspS9I GGNCC 4 cut(s) 244, 481, 991, 1104
AsuC2I CCSGG 2 cut(s) 409, 1267
AsuHPI GGTGA 1 cut(s) 608
AvaI CYCGRG 1 cut(s) 1437
AvaII GGWCC 2 cut(s) 481, 991
BaeGI GKGCMC 1 cut(s) 1633
Bbv12I GWGCWC 2 cut(s) 1237, 1532
BbvCI CCTCAGC 1 cut(s) 1031
BccI CCATC 1 cut(s) 1297
BceAI ACGGC 2 cut(s) 1466, 1569
BcgI CGANNNNNNTGC 2 cut(s) 375, 409
BciT130I CCWGG 3 cut(s) 175, 441, 1327
BclI TGATCA 1 cut(s) 1090
BcnI CCSGG 2 cut(s) 409, 1267
BcoDI GTCTC 5 cut(s) 28, 146, 776, 1043, 1600
BfaI CTAG 4 cut(s) 530, 855, 875, 1020
BfmI CTRYAG 2 cut(s) 122, 1216
BfuAI ACCTGC 1 cut(s) 50
BisI GCNGC 1 cut(s) 1570
BlsI GCNGC 1 cut(s) 1571
BmcAI AGTACT 1 cut(s) 203
Bme1390I CCNGG 5 cut(s) 175, 409, 441, 1267, 1327
Bme18I GGWCC 2 cut(s) 481, 991
BmeRI GACNNNNNGTC 1 cut(s) 487
BmeT110I CYCGRG 1 cut(s) 1437
BmgT120I GGNCC 4 cut(s) 244, 481, 991, 1104
BmiI GGNNCC 3 cut(s) 482, 992, 1368
BmrFI CCNGG 5 cut(s) 175, 409, 441, 1267, 1327
BmsI GCATC 4 cut(s) 105, 666, 854, 1175
BplI GAGNNNNNCTC 4 cut(s) 270, 302, 1014, 1046
Bpu10I CCTNAGC 1 cut(s) 1031
BpuEI CTTGAG 2 cut(s) 149, 518
BpuMI CCSGG 2 cut(s) 409, 1267
BsaBI GATNNNNATC 1 cut(s) 1209
BsaJI CCNNGG 5 cut(s) 247, 408, 1326, 1545, 1668
BsaWI WCCGGW 1 cut(s) 153
BsaXI ACNNNNNCTCC 2 cut(s) 1166, 1196
Bsc4I CCNNNNNNNGG 3 cut(s) 160, 229, 1273
Bse1I ACTGG 1 cut(s) 199
Bse3DI GCAATG 1 cut(s) 234
Bse8I GATNNNNATC 1 cut(s) 1209
BseBI CCWGG 3 cut(s) 175, 441, 1327
BseDI CCNNGG 5 cut(s) 247, 408, 1326, 1545, 1668
BseGI GGATG 8 cut(s) 85, 439, 558, 869, 1006, 1093, 1210, 1678
BseJI GATNNNNATC 1 cut(s) 1209
BseLI CCNNNNNNNGG 3 cut(s) 160, 229, 1273
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 7 cut(s) 659, 852, 885, 1045, 1161, 1248, 1611
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 6 cut(s) 515, 1187, 1245, 1403, 1433, 1436
BseSI GKGCMC 1 cut(s) 1633
BsgI GTGCAG 1 cut(s) 1383
Bsh1236I CGCG 2 cut(s) 60, 456
BshFI GGCC 5 cut(s) 233, 245, 1106, 1501, 1584
BsiHKAI GWGCWC 2 cut(s) 1237, 1532
BsiHKCI CYCGRG 1 cut(s) 1437
BsiSI CCGG 3 cut(s) 154, 408, 1267
BslFI GGGAC 5 cut(s) 122, 295, 361, 494, 1536
BslI CCNNNNNNNGG 3 cut(s) 160, 229, 1273
BsmAI GTCTC 5 cut(s) 28, 146, 776, 1043, 1600
BsmBI CGTCTC 1 cut(s) 28
BsmFI GGGAC 5 cut(s) 122, 295, 361, 494, 1536
BsnI GGCC 5 cut(s) 233, 245, 1106, 1501, 1584
BsoBI CYCGRG 1 cut(s) 1437
Bsp1286I GDGCHC 3 cut(s) 1237, 1532, 1633
Bsp143I GATC 9 cut(s) 100, 225, 292, 330, 436, 631, 1026, 1069, 1090
Bsp19I CCATGG 1 cut(s) 1545
BspACI CCGC 7 cut(s) 34, 209, 273, 478, 642, 1570, 1623
BspANI GGCC 5 cut(s) 233, 245, 1106, 1501, 1584
BspCNI CTCAG 7 cut(s) 658, 851, 886, 1044, 1162, 1249, 1610
BspFNI CGCG 2 cut(s) 60, 456
BspLI GGNNCC 3 cut(s) 482, 992, 1368
BspMI ACCTGC 1 cut(s) 50
BspPI GGATC 3 cut(s) 431, 639, 1021
BspQI GCTCTTC 1 cut(s) 1668
BsrBI CCGCTC 1 cut(s) 644
BsrDI GCAATG 1 cut(s) 234
BsrI ACTGG 1 cut(s) 199
BssECI CCNNGG 5 cut(s) 247, 408, 1326, 1545, 1668
BssMI GATC 9 cut(s) 100, 225, 292, 330, 436, 631, 1026, 1069, 1090
BssT1I CCWWGG 3 cut(s) 247, 1545, 1668
Bst2UI CCWGG 3 cut(s) 175, 441, 1327
Bst4CI ACNGT 1 cut(s) 185
Bst6I CTCTTC 4 cut(s) 271, 464, 1222, 1668
BstAPI GCANNNNNTGC 1 cut(s) 42
BstC8I GCNNGC 4 cut(s) 415, 931, 1116, 1254
BstDSI CCRYGG 1 cut(s) 1545
BstF5I GGATG 8 cut(s) 85, 439, 558, 869, 1006, 1093, 1210, 1678
BstFNI CGCG 2 cut(s) 60, 456
BstHHI GCGC 3 cut(s) 47, 456, 1223
BstKTI GATC 9 cut(s) 103, 228, 295, 333, 439, 634, 1029, 1072, 1093
BstMAI GTCTC 5 cut(s) 28, 146, 776, 1043, 1600
BstMBI GATC 9 cut(s) 100, 225, 292, 330, 436, 631, 1026, 1069, 1090
BstMWI GCNNNNNNNGC 7 cut(s) 42, 124, 404, 967, 1262, 1450, 1620
BstNI CCWGG 3 cut(s) 175, 441, 1327
BstSCI CCNGG 5 cut(s) 173, 407, 439, 1265, 1325
BstSFI CTRYAG 2 cut(s) 122, 1216
BstSLI GKGCMC 1 cut(s) 1633
BstUI CGCG 2 cut(s) 60, 456
BstX2I RGATCY 1 cut(s) 1026
BstXI CCANNNNNNTGG 1 cut(s) 241
BstYI RGATCY 1 cut(s) 1026
BsuRI GGCC 5 cut(s) 233, 245, 1106, 1501, 1584
BtgI CCRYGG 1 cut(s) 1545
BtsCI GGATG 8 cut(s) 85, 439, 558, 869, 1006, 1093, 1210, 1678
BtsI GCAGTG 1 cut(s) 1395
BtsIMutI CAGTG 2 cut(s) 190, 1395
BveI ACCTGC 1 cut(s) 50
Cac8I GCNNGC 4 cut(s) 415, 931, 1116, 1254
CfoI GCGC 3 cut(s) 47, 456, 1223
Cfr13I GGNCC 4 cut(s) 244, 481, 991, 1104
CseI GACGC 1 cut(s) 515
Csp6I GTAC 2 cut(s) 202, 1393
CviAII CATG 6 cut(s) 563, 815, 997, 1084, 1546, 1589
CviQI GTAC 2 cut(s) 202, 1393
DpnI GATC 9 cut(s) 102, 227, 294, 332, 438, 633, 1028, 1071, 1092
DpnII GATC 9 cut(s) 100, 225, 292, 330, 436, 631, 1026, 1069, 1090
DraI TTTAAA 1 cut(s) 1248
DriI GACNNNNNGTC 1 cut(s) 487
Eam1104I CTCTTC 4 cut(s) 271, 464, 1222, 1668
Eam1105I GACNNNNNGTC 1 cut(s) 487
EarI CTCTTC 4 cut(s) 271, 464, 1222, 1668
Eco130I CCWWGG 3 cut(s) 247, 1545, 1668
Eco147I AGGCCT 1 cut(s) 1501
Eco47I GGWCC 2 cut(s) 481, 991
Eco57I CTGAAG 1 cut(s) 470
Eco88I CYCGRG 1 cut(s) 1437
EcoO109I RGGNCCY 1 cut(s) 481
EcoRII CCWGG 3 cut(s) 173, 439, 1325
EcoT14I CCWWGG 3 cut(s) 247, 1545, 1668
ErhI CCWWGG 3 cut(s) 247, 1545, 1668
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 6 cut(s) 566, 818, 1000, 1087, 1549, 1592
FalI AAGNNNNNCTT 4 cut(s) 852, 884, 1355, 1387
FaqI GGGAC 5 cut(s) 122, 295, 361, 494, 1536
FatI CATG 6 cut(s) 562, 814, 996, 1083, 1545, 1588
FauI CCCGC 2 cut(s) 41, 471
FbaI TGATCA 1 cut(s) 1090
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 1 cut(s) 1570
FokI GGATG 8 cut(s) 92, 446, 545, 876, 1013, 1100, 1217, 1685
Fsp4HI GCNGC 1 cut(s) 1570
FspBI CTAG 4 cut(s) 530, 855, 875, 1020
GlaI GCGC 3 cut(s) 46, 455, 1222
GluI GCNGC 1 cut(s) 1570
HaeIII GGCC 5 cut(s) 233, 245, 1106, 1501, 1584
HapII CCGG 3 cut(s) 154, 408, 1267
HgaI GACGC 1 cut(s) 515
HhaI GCGC 3 cut(s) 47, 456, 1223
Hin1II CATG 6 cut(s) 566, 818, 1000, 1087, 1549, 1592
Hin6I GCGC 3 cut(s) 45, 454, 1221
HinP1I GCGC 3 cut(s) 45, 454, 1221
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 2 cut(s) 622, 1116
HpaII CCGG 3 cut(s) 154, 408, 1267
HphI GGTGA 1 cut(s) 608
Hpy166II GTNNAC 6 cut(s) 259, 303, 553, 616, 994, 1395
Hpy188I TCNGA 6 cut(s) 105, 777, 1069, 1095, 1171, 1689
Hpy188III TCNNGA 7 cut(s) 143, 320, 334, 530, 955, 1439, 1457
Hpy8I GTNNAC 6 cut(s) 259, 303, 553, 616, 994, 1395
HpyAV CCTTC 8 cut(s) 154, 334, 494, 511, 934, 1123, 1512, 1620
HpyCH4III ACNGT 1 cut(s) 185
HpyCH4IV ACGT 3 cut(s) 1382, 1638, 1656
HpyCH4V TGCA 8 cut(s) 67, 196, 386, 730, 1158, 1166, 1400, 1472
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 124, 404, 967, 1262, 1450, 1620
HpySE526I ACGT 3 cut(s) 1382, 1638, 1656
Hsp92II CATG 6 cut(s) 566, 818, 1000, 1087, 1549, 1592
HspAI GCGC 3 cut(s) 45, 454, 1221
Ksp22I TGATCA 1 cut(s) 1090
Kzo9I GATC 9 cut(s) 100, 225, 292, 330, 436, 631, 1026, 1069, 1090
LguI GCTCTTC 1 cut(s) 1668
LmnI GCTCC 4 cut(s) 502, 750, 1232, 1441
LweI GCATC 4 cut(s) 105, 666, 854, 1175
MaeI CTAG 4 cut(s) 530, 855, 875, 1020
MaeII ACGT 3 cut(s) 1382, 1638, 1656
MaeIII GTNAC 6 cut(s) 95, 489, 817, 1374, 1538, 1639
MalI GATC 9 cut(s) 102, 227, 294, 332, 438, 633, 1028, 1071, 1092
MbiI CCGCTC 1 cut(s) 644
MboI GATC 9 cut(s) 100, 225, 292, 330, 436, 631, 1026, 1069, 1090
MboII GAAGA 7 cut(s) 288, 349, 481, 825, 986, 1239, 1655
MfeI CAATTG 3 cut(s) 296, 585, 789
MflI RGATCY 1 cut(s) 1026
MhlI GDGCHC 3 cut(s) 1237, 1532, 1633
MlyI GAGTC 3 cut(s) 254, 277, 1602
MmeI TCCRAC 1 cut(s) 1365
MseI TTAA 6 cut(s) 309, 704, 1121, 1247, 1320, 1650
MslI CAYNNNNRTG 1 cut(s) 999
MspA1I CMGCKG 1 cut(s) 1601
MspI CCGG 3 cut(s) 154, 408, 1267
MspR9I CCNGG 5 cut(s) 175, 409, 441, 1267, 1327
MunI CAATTG 3 cut(s) 296, 585, 789
MvaI CCWGG 3 cut(s) 175, 441, 1327
MvnI CGCG 2 cut(s) 60, 456
MwoI GCNNNNNNNGC 7 cut(s) 42, 124, 404, 967, 1262, 1450, 1620
NciI CCSGG 2 cut(s) 409, 1267
NcoI CCATGG 1 cut(s) 1545
NdeII GATC 9 cut(s) 100, 225, 292, 330, 436, 631, 1026, 1069, 1090
NlaIII CATG 6 cut(s) 566, 818, 1000, 1087, 1549, 1592
NlaIV GGNNCC 3 cut(s) 482, 992, 1368
NmuCI GTSAC 2 cut(s) 95, 489
PaqCI CACCTGC 1 cut(s) 50
PceI AGGCCT 1 cut(s) 1501
PciSI GCTCTTC 1 cut(s) 1668
PfeI GAWTC 8 cut(s) 698, 772, 1013, 1046, 1096, 1270, 1344, 1676
PfoI TCCNGGA 1 cut(s) 439
PkrI GCNGC 1 cut(s) 1571
PleI GAGTC 3 cut(s) 254, 277, 1601
PpsI GAGTC 3 cut(s) 254, 277, 1601
PpuMI RGGWCCY 1 cut(s) 481
Psp5II RGGWCCY 1 cut(s) 481
Psp6I CCWGG 3 cut(s) 173, 439, 1325
PspGI CCWGG 3 cut(s) 173, 439, 1325
PspN4I GGNNCC 3 cut(s) 482, 992, 1368
PspPI GGNCC 4 cut(s) 244, 481, 991, 1104
PspPPI RGGWCCY 1 cut(s) 481
PsuI RGATCY 1 cut(s) 1026
PvuII CAGCTG 1 cut(s) 1601
RsaI GTAC 2 cut(s) 203, 1394
RsaNI GTAC 2 cut(s) 202, 1393
RseI CAYNNNNRTG 1 cut(s) 999
SapI GCTCTTC 1 cut(s) 1668
SaqAI TTAA 6 cut(s) 309, 704, 1121, 1247, 1320, 1650
SatI GCNGC 1 cut(s) 1570
Sau3AI GATC 9 cut(s) 100, 225, 292, 330, 436, 631, 1026, 1069, 1090
Sau96I GGNCC 4 cut(s) 244, 481, 991, 1104
ScaI AGTACT 1 cut(s) 203
SchI GAGTC 3 cut(s) 254, 277, 1602
ScrFI CCNGG 5 cut(s) 175, 409, 441, 1267, 1327
SduI GDGCHC 3 cut(s) 1237, 1532, 1633
SfaNI GCATC 4 cut(s) 105, 666, 854, 1175
SfcI CTRYAG 2 cut(s) 122, 1216
SinI GGWCC 2 cut(s) 481, 991
SmiMI CAYNNNNRTG 1 cut(s) 999
SmlI CTYRAG 2 cut(s) 128, 497
SmoI CTYRAG 2 cut(s) 128, 497
SseBI AGGCCT 1 cut(s) 1501
SsiI CCGC 7 cut(s) 34, 209, 273, 478, 642, 1570, 1623
SspMI CTAG 4 cut(s) 530, 855, 875, 1020
StuI AGGCCT 1 cut(s) 1501
StyD4I CCNGG 5 cut(s) 173, 407, 439, 1265, 1325
StyI CCWWGG 3 cut(s) 247, 1545, 1668
TaaI ACNGT 1 cut(s) 185
TaiI ACGT 3 cut(s) 1385, 1641, 1659
TaqI TCGA 2 cut(s) 144, 1299
TatI WGTACW 2 cut(s) 201, 1392
TauI GCSGC 1 cut(s) 1572
TfiI GAWTC 8 cut(s) 698, 772, 1013, 1046, 1096, 1270, 1344, 1676
Tru1I TTAA 6 cut(s) 309, 704, 1121, 1247, 1320, 1650
Tru9I TTAA 6 cut(s) 309, 704, 1121, 1247, 1320, 1650
TscAI CASTG 2 cut(s) 190, 1402
TseFI GTSAC 2 cut(s) 95, 489
Tsp45I GTSAC 2 cut(s) 95, 489
TspDTI ATGAA 8 cut(s) 96, 579, 690, 810, 953, 1206, 1212, 1221
TspGWI ACGGA 2 cut(s) 10, 644
TspRI CASTG 2 cut(s) 190, 1402
VpaK11BI GGWCC 2 cut(s) 481, 991
XapI RAATTY 3 cut(s) 978, 1243, 1303
XbaI TCTAGA 1 cut(s) 529
XmiI GTMKAC 1 cut(s) 302
XspI CTAG 4 cut(s) 530, 855, 875, 1020
ZrmI AGTACT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.