AT2G40250

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013832
Physical Location & Seq
Forward (+)
16813297 .. 16815331
2035 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G40250.1

Sequence Viewer

Length: 1086 bp
ATGAACCGTAATCAACACAAACCAATGTTTGTTACATTCCTCATCAATATTCTTCTCCTCCAACTTCTAAACCTTACCAATGCATCACCATCCCCACCAATAACAGCCCTATACGCCTTCGGCGACTCCACAGTGGACTCCGGTAACAACAATTACATTCCCACTCTTTTCCAGAGCAACCATCCTCCCTATGGTAAATCCTTTCCGTCCAAACTCTCCACCGGAAGGTTCTCCGACGGAAAACTCGCCACCGACTTCATAGTCTCCTCTCTAGGACTCAAACCCACTCTACCAGCTTACCTCAACCCTTCGGTCAAACCTGTAGATCTTTTGACCGGCGTTAGTTTCGCCTCGGCCGGAGGTGGTTTAGATGATCGGACGGCAAAGTCATCATTGACTATTACCATGGACAAGCAATGGAGTTACTTCGAAGAGGCGCTGGGTAAAATGAAGAGTTTGGTTGGAGACTCGGAGACTAATCGGGTGATTAAGAATGCTGTGTTCGTCATTAGTGCGGGGACTAATGATATGATCTTTAATGTTTACGATCATGTTCTTGGGAGTTTGATCTCCGTTTCGGATTATCAAGATTCTCTACTAACCAAAGTCGAAGTTTTTGTCCAGAGACTATACGAGGCAGGAGCACGAAGGATTACAATAGCTGGACTACCACCAATCGGATGCCTTCCGGTGCAAGTAACACTTACTAGCATCAATACTCCTCGCATCTTCCATCATCGGATCTGTACGGAACATCAGAACGATGATTCTCGGGTTTACAATCAAAAGCTACAGAAGCTAATCTTCGGTCTCAGTCAGAGGTTTCGAGGTTCCAAAGTTCTTTACCTTGATATCTACTCTCCGTTAATAGACATGATCAAACATCCTCGTAAATACGGACTAGAGGAAACATTGAGAGGATGTTGTGGGACAGGGCTACTTGAGGCAGGACCTCTATGCCAACCATTGTCTCGGACTTGTGACGATGTTTCCAAGTATTTATTCTTTGATTCTGTGCATCCTTCACAGACGGCTTATTCTGTCATCGCTAGTTTCGCCTTACAAAAATTGTTTCCTCTCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

361

Amino Acids

39.88

Weight (kDa)

8.76

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 351 9.3e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40250
fragaria_vesca FvH4_7g24850 FvH4_7g24850
malus_domestica MD01G1156400.v1.1 MD07G1225100.v1.1
prunus_persica Prupe.2G253600_v2.0.a1
pyrus_communis pycom07g20380
rosa_chinensis RchiOBHm_Chr1g0370731
rosa_laevigata RLG00000027015
rosa_multiflora Rmu_co8466073.1_g000001 Rmu_sc0004550.1_g000013
rosa_rugosa Rorug01G0358700
rosa_samantha Rh1AG368400 Rh1BG331900 Rh1CG345800 Rh1DG363300
rosa_wichuraiana Rw1G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 260, 385
AciI CCGC 1 cut(s) 515
AclWI GGATC 1 cut(s) 749
AcoI YGGCCR 1 cut(s) 354
AfaI GTAC 1 cut(s) 748
AfiI CCNNNNNNNGG 3 cut(s) 191, 225, 677
AjuI GAANNNNNNNTTGG 2 cut(s) 986, 1018
AluBI AGCT 4 cut(s) 296, 662, 790, 799
AluI AGCT 4 cut(s) 296, 662, 790, 799
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 6 cut(s) 268, 459, 467, 619, 815, 975
AlwI GGATC 1 cut(s) 749
Ama87I CYCGRG 1 cut(s) 771
AoxI GGCC 1 cut(s) 354
ArsI GACNNNNNNTTYG 2 cut(s) 603, 635
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 1 cut(s) 950
AsuHPI GGTGA 2 cut(s) 78, 496
AsuII TTCGAA 1 cut(s) 429
AvaI CYCGRG 1 cut(s) 771
AvaII GGWCC 1 cut(s) 950
Bbv12I GWGCWC 1 cut(s) 646
BccI CCATC 3 cut(s) 97, 189, 741
BceAI ACGGC 2 cut(s) 396, 1047
BclI TGATCA 1 cut(s) 876
BcoDI GTCTC 6 cut(s) 268, 459, 467, 619, 815, 975
BfaI CTAG 4 cut(s) 272, 708, 902, 1050
BfmI CTRYAG 2 cut(s) 321, 791
BfoI RGCGCY 1 cut(s) 440
BglII AGATCT 1 cut(s) 325
Bme18I GGWCC 1 cut(s) 950
BmeT110I CYCGRG 1 cut(s) 771
BmgT120I GGNCC 1 cut(s) 950
BmiI GGNNCC 1 cut(s) 832
BmsI GCATC 5 cut(s) 92, 671, 720, 735, 1027
Bpu14I TTCGAA 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 962
BsaI GGTCTC 1 cut(s) 815
BsaJI CCNNGG 2 cut(s) 351, 405
BsaWI WCCGGW 3 cut(s) 140, 221, 688
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 225, 677
Bse118I RCCGGY 1 cut(s) 335
Bse3DI GCAATG 1 cut(s) 422
BseDI CCNNGG 2 cut(s) 351, 405
BseGI GGATG 6 cut(s) 89, 181, 686, 883, 926, 1018
BseLI CCNNNNNNNGG 3 cut(s) 191, 225, 677
BseMI GCAATG 1 cut(s) 422
BseMII CTCAG 1 cut(s) 826
BseRI GAGGAG 4 cut(s) 47, 256, 711, 1070
BseX3I CGGCCG 1 cut(s) 354
BseYI CCCAGC 1 cut(s) 439
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 1 cut(s) 356
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 646
BsiHKCI CYCGRG 1 cut(s) 771
BsiSI CCGG 5 cut(s) 141, 222, 336, 357, 689
BslFI GGGAC 2 cut(s) 532, 943
BslI CCNNNNNNNGG 3 cut(s) 191, 225, 677
BsmAI GTCTC 6 cut(s) 268, 459, 467, 619, 815, 975
BsmFI GGGAC 2 cut(s) 532, 943
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 1 cut(s) 356
Bso31I GGTCTC 1 cut(s) 815
BsoBI CYCGRG 1 cut(s) 771
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 7 cut(s) 325, 373, 531, 547, 567, 741, 876
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 1 cut(s) 515
BspANI GGCC 1 cut(s) 356
BspCNI CTCAG 1 cut(s) 825
BspLI GGNNCC 1 cut(s) 832
BspPI GGATC 1 cut(s) 749
BspT104I TTCGAA 1 cut(s) 429
BspTNI GGTCTC 1 cut(s) 815
BsrDI GCAATG 1 cut(s) 422
BsrFI RCCGGY 1 cut(s) 335
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 2 cut(s) 351, 405
BssMI GATC 7 cut(s) 325, 373, 531, 547, 567, 741, 876
BssT1I CCWWGG 1 cut(s) 405
Bst4CI ACNGT 2 cut(s) 8, 133
Bst6I CTCTTC 2 cut(s) 426, 446
BstBI TTCGAA 1 cut(s) 429
BstDEI CTNAG 1 cut(s) 812
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 6 cut(s) 89, 181, 686, 883, 926, 1018
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 7 cut(s) 328, 376, 534, 550, 570, 744, 879
BstMAI GTCTC 6 cut(s) 268, 459, 467, 619, 815, 975
BstMBI GATC 7 cut(s) 325, 373, 531, 547, 567, 741, 876
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 3 cut(s) 113, 796, 1055
BstSFI CTRYAG 2 cut(s) 321, 791
BstX2I RGATCY 2 cut(s) 325, 741
BstYI RGATCY 2 cut(s) 325, 741
BstZI CGGCCG 1 cut(s) 354
BsuRI GGCC 1 cut(s) 356
BtgI CCRYGG 1 cut(s) 405
BtgZI GCGATG 1 cut(s) 1030
BtsCI GGATG 6 cut(s) 89, 181, 686, 883, 926, 1018
BtsIMutI CAGTG 1 cut(s) 138
CfoI GCGC 1 cut(s) 439
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 950
Csp6I GTAC 1 cut(s) 747
CviAII CATG 3 cut(s) 406, 551, 874
CviJI RGCY 8 cut(s) 107, 296, 356, 662, 790, 799, 937, 1034
CviKI_1 RGCY 8 cut(s) 107, 296, 356, 662, 790, 799, 937, 1034
CviQI GTAC 1 cut(s) 747
DdeI CTNAG 1 cut(s) 812
DpnI GATC 7 cut(s) 327, 375, 533, 549, 569, 743, 878
DpnII GATC 7 cut(s) 325, 373, 531, 547, 567, 741, 876
DrdI GACNNNNNNGTC 2 cut(s) 260, 385
DseDI GACNNNNNNGTC 2 cut(s) 260, 385
EaeI YGGCCR 1 cut(s) 354
EagI CGGCCG 1 cut(s) 354
Eam1104I CTCTTC 2 cut(s) 426, 446
EarI CTCTTC 2 cut(s) 426, 446
EclXI CGGCCG 1 cut(s) 354
Eco130I CCWWGG 1 cut(s) 405
Eco31I GGTCTC 1 cut(s) 815
Eco32I GATATC 1 cut(s) 853
Eco47I GGWCC 1 cut(s) 950
Eco52I CGGCCG 1 cut(s) 354
Eco88I CYCGRG 1 cut(s) 771
EcoO109I RGGNCCY 1 cut(s) 950
EcoRV GATATC 1 cut(s) 853
EcoT14I CCWWGG 1 cut(s) 405
EcoT22I ATGCAT 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 3 cut(s) 409, 554, 877
FaiI YATR 9 cut(s) 112, 192, 260, 407, 530, 552, 631, 875, 958
FalI AAGNNNNNCTT 4 cut(s) 687, 719, 788, 820
FaqI GGGAC 2 cut(s) 532, 943
FatI CATG 3 cut(s) 405, 550, 873
FauI CCCGC 1 cut(s) 508
FbaI TGATCA 1 cut(s) 876
FokI GGATG 6 cut(s) 76, 168, 693, 870, 933, 1005
FspBI CTAG 4 cut(s) 272, 708, 902, 1050
GlaI GCGC 1 cut(s) 438
GsaI CCCAGC 1 cut(s) 443
HaeII RGCGCY 1 cut(s) 440
HaeIII GGCC 1 cut(s) 356
HapII CCGG 5 cut(s) 141, 222, 336, 357, 689
HhaI GCGC 1 cut(s) 439
Hin1II CATG 3 cut(s) 409, 554, 877
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HinfI GANTC 7 cut(s) 125, 137, 276, 467, 590, 767, 1010
HpaII CCGG 5 cut(s) 141, 222, 336, 357, 689
HphI GGTGA 2 cut(s) 78, 496
Hpy166II GTNNAC 3 cut(s) 136, 544, 778
Hpy188I TCNGA 9 cut(s) 235, 378, 472, 580, 680, 741, 759, 819, 975
Hpy188III TCNNGA 3 cut(s) 172, 587, 622
Hpy8I GTNNAC 3 cut(s) 136, 544, 778
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 6 cut(s) 127, 219, 318, 642, 695, 1032
HpyCH4III ACNGT 2 cut(s) 8, 133
HpyCH4V TGCA 3 cut(s) 83, 694, 1018
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 796, 1055
HpyF3I CTNAG 1 cut(s) 812
Hsp92II CATG 3 cut(s) 409, 554, 877
HspAI GCGC 1 cut(s) 437
Ksp22I TGATCA 1 cut(s) 876
Kzo9I GATC 7 cut(s) 325, 373, 531, 547, 567, 741, 876
LmnI GCTCC 1 cut(s) 641
LweI GCATC 5 cut(s) 92, 671, 720, 735, 1027
MaeI CTAG 4 cut(s) 272, 708, 902, 1050
MaeIII GTNAC 5 cut(s) 31, 143, 422, 697, 980
MalI GATC 7 cut(s) 327, 375, 533, 549, 569, 743, 878
MboI GATC 7 cut(s) 325, 373, 531, 547, 567, 741, 876
MboII GAAGA 5 cut(s) 44, 443, 463, 721, 796
MflI RGATCY 2 cut(s) 325, 741
MhlI GDGCHC 1 cut(s) 646
MluCI AATT 2 cut(s) 151, 1067
MlyI GAGTC 4 cut(s) 119, 131, 270, 461
MmeI TCCRAC 3 cut(s) 85, 258, 442
Mph1103I ATGCAT 1 cut(s) 85
MseI TTAA 3 cut(s) 489, 537, 866
MspI CCGG 5 cut(s) 141, 222, 336, 357, 689
Mva1269I GAATGC 1 cut(s) 499
MwoI GCNNNNNNNGC 3 cut(s) 113, 796, 1055
NcoI CCATGG 1 cut(s) 405
NdeII GATC 7 cut(s) 325, 373, 531, 547, 567, 741, 876
NlaIII CATG 3 cut(s) 409, 554, 877
NlaIV GGNNCC 1 cut(s) 832
NmeAIII GCCGAG 1 cut(s) 332
NmuCI GTSAC 1 cut(s) 980
NsiI ATGCAT 1 cut(s) 85
NspV TTCGAA 1 cut(s) 429
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 1 cut(s) 499
PfeI GAWTC 3 cut(s) 590, 767, 1010
PleI GAGTC 4 cut(s) 119, 131, 270, 461
PpsI GAGTC 4 cut(s) 119, 131, 270, 461
PpuMI RGGWCCY 1 cut(s) 950
Psp5II RGGWCCY 1 cut(s) 950
PspFI CCCAGC 1 cut(s) 439
PspN4I GGNNCC 1 cut(s) 832
PspPI GGNCC 1 cut(s) 950
PspPPI RGGWCCY 1 cut(s) 950
PsuI RGATCY 2 cut(s) 325, 741
RsaI GTAC 1 cut(s) 748
RsaNI GTAC 1 cut(s) 747
SaqAI TTAA 3 cut(s) 489, 537, 866
Sau3AI GATC 7 cut(s) 325, 373, 531, 547, 567, 741, 876
Sau96I GGNCC 1 cut(s) 950
SchI GAGTC 4 cut(s) 119, 131, 270, 461
SduI GDGCHC 1 cut(s) 646
SfaNI GCATC 5 cut(s) 92, 671, 720, 735, 1027
SfcI CTRYAG 2 cut(s) 321, 791
SfuI TTCGAA 1 cut(s) 429
SinI GGWCC 1 cut(s) 950
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
Sse9I AATT 2 cut(s) 151, 1067
SsiI CCGC 1 cut(s) 515
SspI AATATT 1 cut(s) 49
SspMI CTAG 4 cut(s) 272, 708, 902, 1050
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 2 cut(s) 8, 133
TaqI TCGA 3 cut(s) 429, 609, 826
TaqII GACCGA 2 cut(s) 301, 797
TasI AATT 2 cut(s) 151, 1067
TfiI GAWTC 3 cut(s) 590, 767, 1010
Tru1I TTAA 3 cut(s) 489, 537, 866
Tru9I TTAA 3 cut(s) 489, 537, 866
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 1 cut(s) 980
Tsp45I GTSAC 1 cut(s) 980
TspDTI ATGAA 3 cut(s) 17, 247, 464
TspGWI ACGGA 6 cut(s) 195, 252, 562, 764, 852, 912
TspRI CASTG 1 cut(s) 138
VpaK11BI GGWCC 1 cut(s) 950
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
XspI CTAG 4 cut(s) 272, 708, 902, 1050
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.