Rh1DG363300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0013832
Physical Location & Seq
Forward (+)
58995841 .. 58997765
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG363300.1

Sequence Viewer

Length: 1071 bp
ATGTTCACACTACTATTGGCCACCTTGATAATCCTACCGCCATCTCCGATCAGTGCCTCCTCTCCATCTGTTTCTGCTCTCTTCGCTTTCGGCGATTCCACGGTGGATTCTGGCATGAACAACGACCTCATGCCTTTCGTTCGGTGCGACCATCCCCCTTACGGCAGAGACCTGCCGGAACACGTCCCCACCGGAAGGTACTCCAATGGAAAGATTGCAACTGACTTTATGGCCTCAAACTTGGGACTCAAGGACCTCTTGCCCGCTTACCTCGACCCGAAATTGACCGACTATGATTTGCTCACCGGGGTCAGTTTCGCGTCGGGAACTTGCGGCCTAGATGATCTGACCTTGGAGTTGAGCCACGTCTATAGTATGGGGGATCAGTTGGAGTATTTTGATGAAGCCGTAGTGAGGATGAAGATAAGTGTTGGGGAAAAGAAGGGTAGAGAGATTGTGGAAAATGCACTGTTTGTGGTTAGTGTTGGGACGAATGACTTGATGCTGAACGTTTATGAGTTTCCTCTAACCGAGAGGAAAACTCAGTTTACGTCTACAGACCAGTACTCTGATTTTCTGCTCCAGAGACTTGGGTCTTTTATTCAGGCTCTGTACAGTAGGGGAGCACGGCGGTTCATGGTGGCAGGTCTTCCACCGATCGGATGCCTTCCGCTTCAACGGACACTGAGCGGCATCATCAATCTGCGCCCTCAGCGCCTGTGCGTCACGCAGCAAAACATAGACTGTCAGACCTACAACTCCAAGCTCCAGGCCCTTCTCTCAAGTTTGCAAACCACACTACCACTCCCTAGTTCAAGGCTAGTCTATTTTGACATCTACAACCCCATCATGGACATGTACAACAACCCAGATAAATATGGGTTTGCGCATATACATGAAGGATGCTGTGGAACTGGGATGCTTGAGTTGGGGCCTCTGTGCACAGAGCTTTCGCAGACGTGCCCCGACGCGTCGAAGTATCTTTTCTGGGATGCAGTTCATCTTACTGAAGCAGCATTTTTCGTCATTGCTGACTATGCTCATAAGACTGTCTTGCAGTACCTTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

356

Amino Acids

39.5

Weight (kDa)

4.97

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 27 - 344 3.5e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40250
fragaria_vesca FvH4_7g24850 FvH4_7g24850
malus_domestica MD01G1156400.v1.1 MD07G1225100.v1.1
prunus_persica Prupe.2G253600_v2.0.a1
pyrus_communis pycom07g20380
rosa_chinensis RchiOBHm_Chr1g0370731
rosa_laevigata RLG00000027015
rosa_multiflora Rmu_co8466073.1_g000001 Rmu_sc0004550.1_g000013
rosa_rugosa Rorug01G0358700
rosa_samantha Rh1AG368400 Rh1BG331900 Rh1CG345800 Rh1DG363300
rosa_wichuraiana Rw1G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 888
Acc36I ACCTGC 2 cut(s) 180, 635
AccBSI CCGCTC 1 cut(s) 690
AccI GTMKAC 1 cut(s) 554
AccII CGCG 2 cut(s) 320, 971
AciI CCGC 6 cut(s) 38, 264, 333, 631, 671, 690
AclI AACGTT 1 cut(s) 510
AclWI GGATC 1 cut(s) 390
AcoI YGGCCR 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 1029
AfaI GTAC 5 cut(s) 200, 566, 614, 860, 1061
AfiI CCNNNNNNNGG 5 cut(s) 161, 195, 414, 659, 850
AflIII ACRYGT 3 cut(s) 181, 855, 969
AgsI TTSAA 2 cut(s) 677, 816
AjiI CACGTC 3 cut(s) 184, 367, 960
AjnI CCWGG 1 cut(s) 768
AluBI AGCT 2 cut(s) 766, 949
AluI AGCT 2 cut(s) 766, 949
Alw21I GWGCWC 2 cut(s) 628, 944
Alw26I GTCTC 2 cut(s) 162, 580
Alw44I GTGCAC 1 cut(s) 940
AlwI GGATC 1 cut(s) 390
AlwNI CAGNNNCTG 2 cut(s) 610, 718
AoxI GGCC 5 cut(s) 18, 231, 334, 771, 932
ApaLI GTGCAC 1 cut(s) 940
ApeKI GCWGC 2 cut(s) 730, 1013
AspLEI GCGC 3 cut(s) 708, 717, 889
AspS9I GGNCC 3 cut(s) 253, 772, 932
AsuC2I CCSGG 1 cut(s) 307
AsuHPI GGTGA 1 cut(s) 295
AvaII GGWCC 1 cut(s) 253
BaeGI GKGCMC 2 cut(s) 944, 965
BalI TGGCCA 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 641
Bbv12I GWGCWC 2 cut(s) 628, 944
BbvCI CCTCAGC 1 cut(s) 711
BbvI GCAGC 2 cut(s) 742, 1025
BccI CCATC 4 cut(s) 49, 73, 159, 854
BceAI ACGGC 3 cut(s) 178, 392, 644
BciT130I CCWGG 1 cut(s) 770
BcnI CCSGG 1 cut(s) 307
BcoDI GTCTC 2 cut(s) 162, 580
BfaI CTAG 3 cut(s) 338, 810, 821
BfmI CTRYAG 2 cut(s) 370, 555
BfoI RGCGCY 1 cut(s) 718
BfuAI ACCTGC 2 cut(s) 180, 635
BisI GCNGC 4 cut(s) 334, 691, 731, 1014
BlsI GCNGC 4 cut(s) 335, 692, 732, 1015
BmcAI AGTACT 1 cut(s) 566
Bme1390I CCNGG 2 cut(s) 307, 770
Bme18I GGWCC 1 cut(s) 253
BmgBI CACGTC 3 cut(s) 184, 367, 960
BmgT120I GGNCC 3 cut(s) 253, 772, 932
BmiI GGNNCC 1 cut(s) 933
BmrFI CCNGG 2 cut(s) 307, 770
BmrI ACTGGG 1 cut(s) 924
BmsI GCATC 6 cut(s) 492, 653, 702, 893, 909, 982
BmuI ACTGGG 1 cut(s) 924
BoxI GACNNNNGTC 1 cut(s) 592
BpiI GAAGAC 1 cut(s) 641
BplI GAGNNNNNCTC 2 cut(s) 526, 558
BpmI CTGGAG 2 cut(s) 566, 752
Bpu10I CCTNAGC 1 cut(s) 711
BpuEI CTTGAG 3 cut(s) 233, 766, 944
BpuMI CCSGG 1 cut(s) 307
BsaI GGTCTC 1 cut(s) 162
BsaJI CCNNGG 3 cut(s) 99, 306, 351
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 2 cut(s) 789, 819
Bsc4I CCNNNNNNNGG 5 cut(s) 161, 195, 414, 659, 850
Bse1I ACTGG 2 cut(s) 562, 919
Bse3DI GCAATG 1 cut(s) 1026
BseBI CCWGG 1 cut(s) 770
BseDI CCNNGG 3 cut(s) 99, 306, 351
BseGI GGATG 6 cut(s) 151, 423, 668, 908, 924, 997
BseLI CCNNNNNNNGG 5 cut(s) 161, 195, 414, 659, 850
BseMI GCAATG 1 cut(s) 1026
BseMII CTCAG 3 cut(s) 557, 677, 725
BseNI ACTGG 2 cut(s) 562, 919
BseRI GAGGAG 1 cut(s) 49
BseSI GKGCMC 2 cut(s) 944, 965
BseXI GCAGC 2 cut(s) 742, 1025
Bsh1236I CGCG 2 cut(s) 320, 971
Bsh1285I CGRYCG 1 cut(s) 660
BshFI GGCC 5 cut(s) 20, 233, 336, 773, 934
BsiEI CGRYCG 1 cut(s) 660
BsiHKAI GWGCWC 2 cut(s) 628, 944
BsiSI CCGG 3 cut(s) 176, 192, 306
BslFI GGGAC 3 cut(s) 170, 258, 502
BslI CCNNNNNNNGG 5 cut(s) 161, 195, 414, 659, 850
BsmAI GTCTC 2 cut(s) 162, 580
BsmFI GGGAC 3 cut(s) 170, 258, 502
BsnI GGCC 5 cut(s) 20, 233, 336, 773, 934
Bso31I GGTCTC 1 cut(s) 162
Bsp1286I GDGCHC 3 cut(s) 628, 944, 965
Bsp1407I TGTACA 2 cut(s) 612, 858
Bsp143I GATC 4 cut(s) 48, 343, 382, 657
BspACI CCGC 6 cut(s) 38, 264, 333, 631, 671, 690
BspANI GGCC 5 cut(s) 20, 233, 336, 773, 934
BspCNI CTCAG 3 cut(s) 556, 678, 724
BspFNI CGCG 2 cut(s) 320, 971
BspLI GGNNCC 1 cut(s) 933
BspMI ACCTGC 2 cut(s) 180, 635
BspPI GGATC 1 cut(s) 390
BspTNI GGTCTC 1 cut(s) 162
BsrBI CCGCTC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 1026
BsrGI TGTACA 2 cut(s) 612, 858
BsrI ACTGG 2 cut(s) 562, 919
BssECI CCNNGG 3 cut(s) 99, 306, 351
BssMI GATC 4 cut(s) 48, 343, 382, 657
BssT1I CCWWGG 1 cut(s) 351
Bst2UI CCWGG 1 cut(s) 770
Bst4CI ACNGT 5 cut(s) 103, 471, 617, 746, 1051
Bst6I CTCTTC 1 cut(s) 86
BstAUI TGTACA 2 cut(s) 612, 858
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 4 cut(s) 543, 686, 711, 1064
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 6 cut(s) 151, 423, 668, 908, 924, 997
BstFNI CGCG 2 cut(s) 320, 971
BstH2I RGCGCY 1 cut(s) 718
BstHHI GCGC 3 cut(s) 708, 717, 889
BstKTI GATC 4 cut(s) 51, 346, 385, 660
BstMAI GTCTC 2 cut(s) 162, 580
BstMBI GATC 4 cut(s) 48, 343, 382, 657
BstMCI CGRYCG 1 cut(s) 660
BstMWI GCNNNNNNNGC 4 cut(s) 83, 712, 714, 1037
BstNI CCWGG 1 cut(s) 770
BstNSI RCATGY 1 cut(s) 859
BstPAI GACNNNNGTC 1 cut(s) 592
BstSCI CCNGG 2 cut(s) 305, 768
BstSFI CTRYAG 2 cut(s) 370, 555
BstSLI GKGCMC 2 cut(s) 944, 965
BstUI CGCG 2 cut(s) 320, 971
BstV1I GCAGC 2 cut(s) 742, 1025
BstV2I GAAGAC 1 cut(s) 641
BstXI CCANNNNNNTGG 1 cut(s) 590
BsuRI GGCC 5 cut(s) 20, 233, 336, 773, 934
BtgI CCRYGG 1 cut(s) 99
BtrI CACGTC 3 cut(s) 184, 367, 960
BtsCI GGATG 6 cut(s) 151, 423, 668, 908, 924, 997
BtsIMutI CAGTG 3 cut(s) 58, 467, 683
BveI ACCTGC 2 cut(s) 180, 635
Cac8I GCNNGC 1 cut(s) 264
CaiI CAGNNNCTG 2 cut(s) 610, 718
CfoI GCGC 3 cut(s) 708, 717, 889
Cfr13I GGNCC 3 cut(s) 253, 772, 932
CseI GACGC 4 cut(s) 309, 712, 960, 977
Csp6I GTAC 5 cut(s) 199, 565, 613, 859, 1060
CviAII CATG 6 cut(s) 115, 130, 637, 850, 856, 896
CviQI GTAC 5 cut(s) 199, 565, 613, 859, 1060
DdeI CTNAG 4 cut(s) 543, 686, 711, 1064
DpnI GATC 4 cut(s) 50, 345, 384, 659
DpnII GATC 4 cut(s) 48, 343, 382, 657
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
Eco130I CCWWGG 1 cut(s) 351
Eco31I GGTCTC 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 253
Eco57I CTGAAG 1 cut(s) 1029
EcoO109I RGGNCCY 3 cut(s) 253, 772, 932
EcoRII CCWGG 1 cut(s) 768
EcoT14I CCWWGG 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 6 cut(s) 118, 133, 640, 853, 859, 899
FalI AAGNNNNNCTT 4 cut(s) 242, 274, 1037, 1069
FaqI GGGAC 3 cut(s) 170, 258, 502
FatI CATG 6 cut(s) 114, 129, 636, 849, 855, 895
FauI CCCGC 1 cut(s) 271
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 4 cut(s) 334, 691, 731, 1014
FokI GGATG 6 cut(s) 138, 430, 675, 915, 931, 1004
Fsp4HI GCNGC 4 cut(s) 334, 691, 731, 1014
FspBI CTAG 3 cut(s) 338, 810, 821
FspI TGCGCA 1 cut(s) 888
GlaI GCGC 3 cut(s) 707, 716, 888
GluI GCNGC 4 cut(s) 334, 691, 731, 1014
GsuI CTGGAG 2 cut(s) 566, 752
HaeII RGCGCY 1 cut(s) 718
HaeIII GGCC 5 cut(s) 20, 233, 336, 773, 934
HapII CCGG 3 cut(s) 176, 192, 306
HgaI GACGC 4 cut(s) 309, 712, 960, 977
HhaI GCGC 3 cut(s) 708, 717, 889
Hin1II CATG 6 cut(s) 118, 133, 640, 853, 859, 899
Hin6I GCGC 3 cut(s) 706, 715, 887
HinP1I GCGC 3 cut(s) 706, 715, 887
HinfI GANTC 3 cut(s) 95, 107, 246
HpaII CCGG 3 cut(s) 176, 192, 306
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 4 cut(s) 6, 549, 555, 942
Hpy188I TCNGA 5 cut(s) 48, 348, 571, 662, 750
Hpy188III TCNNGA 2 cut(s) 324, 583
Hpy8I GTNNAC 4 cut(s) 6, 549, 555, 942
Hpy99I CGWCG 3 cut(s) 325, 971, 976
HpyAV CCTTC 5 cut(s) 189, 436, 677, 785, 893
HpyCH4III ACNGT 5 cut(s) 103, 471, 617, 746, 1051
HpyCH4IV ACGT 5 cut(s) 183, 366, 510, 551, 959
HpyCH4V TGCA 6 cut(s) 218, 467, 790, 942, 995, 1057
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 712, 714, 1037
HpyF3I CTNAG 4 cut(s) 543, 686, 711, 1064
HpySE526I ACGT 5 cut(s) 183, 366, 510, 551, 959
Hsp92II CATG 6 cut(s) 118, 133, 640, 853, 859, 899
HspAI GCGC 3 cut(s) 706, 715, 887
Kzo9I GATC 4 cut(s) 48, 343, 382, 657
LmnI GCTCC 3 cut(s) 585, 623, 771
Lsp1109I GCAGC 2 cut(s) 742, 1025
LweI GCATC 6 cut(s) 492, 653, 702, 893, 909, 982
MaeI CTAG 3 cut(s) 338, 810, 821
MaeII ACGT 5 cut(s) 183, 366, 510, 551, 959
MaeIII GTNAC 1 cut(s) 724
MalI GATC 4 cut(s) 50, 345, 384, 659
MbiI CCGCTC 1 cut(s) 690
MboI GATC 4 cut(s) 48, 343, 382, 657
MboII GAAGA 3 cut(s) 73, 433, 641
MhlI GDGCHC 3 cut(s) 628, 944, 965
MlsI TGGCCA 1 cut(s) 20
MluCI AATT 1 cut(s) 281
MluI ACGCGT 1 cut(s) 969
MluNI TGGCCA 1 cut(s) 20
MlyI GAGTC 1 cut(s) 240
MmeI TCCRAC 1 cut(s) 369
Mox20I TGGCCA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 20
MslI CAYNNNNRTG 2 cut(s) 854, 894
Msp20I TGGCCA 1 cut(s) 20
MspI CCGG 3 cut(s) 176, 192, 306
MspR9I CCNGG 2 cut(s) 307, 770
MvaI CCWGG 1 cut(s) 770
MvnI CGCG 2 cut(s) 320, 971
MwoI GCNNNNNNNGC 4 cut(s) 83, 712, 714, 1037
NciI CCSGG 1 cut(s) 307
NdeII GATC 4 cut(s) 48, 343, 382, 657
NlaIII CATG 6 cut(s) 118, 133, 640, 853, 859, 899
NlaIV GGNNCC 1 cut(s) 933
NmuCI GTSAC 1 cut(s) 724
NsbI TGCGCA 1 cut(s) 888
NspI RCATGY 1 cut(s) 859
PciI ACATGT 1 cut(s) 855
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 144
PfeI GAWTC 2 cut(s) 95, 107
PkrI GCNGC 4 cut(s) 335, 692, 732, 1015
Ple19I CGATCG 1 cut(s) 660
PleI GAGTC 1 cut(s) 240
PpsI GAGTC 1 cut(s) 240
PpuMI RGGWCCY 1 cut(s) 253
PscI ACATGT 1 cut(s) 855
PshAI GACNNNNGTC 1 cut(s) 592
Psp1406I AACGTT 1 cut(s) 510
Psp5II RGGWCCY 1 cut(s) 253
Psp6I CCWGG 1 cut(s) 768
PspGI CCWGG 1 cut(s) 768
PspN4I GGNNCC 1 cut(s) 933
PspPI GGNCC 3 cut(s) 253, 772, 932
PspPPI RGGWCCY 1 cut(s) 253
PstNI CAGNNNCTG 2 cut(s) 610, 718
PvuI CGATCG 1 cut(s) 660
RsaI GTAC 5 cut(s) 200, 566, 614, 860, 1061
RsaNI GTAC 5 cut(s) 199, 565, 613, 859, 1060
RseI CAYNNNNRTG 2 cut(s) 854, 894
SatI GCNGC 4 cut(s) 334, 691, 731, 1014
Sau3AI GATC 4 cut(s) 48, 343, 382, 657
Sau96I GGNCC 3 cut(s) 253, 772, 932
ScaI AGTACT 1 cut(s) 566
SchI GAGTC 1 cut(s) 240
ScrFI CCNGG 2 cut(s) 307, 770
SduI GDGCHC 3 cut(s) 628, 944, 965
SfaNI GCATC 6 cut(s) 492, 653, 702, 893, 909, 982
SfcI CTRYAG 2 cut(s) 370, 555
SinI GGWCC 1 cut(s) 253
SmiMI CAYNNNNRTG 2 cut(s) 854, 894
SmlI CTYRAG 3 cut(s) 248, 781, 923
SmoI CTYRAG 3 cut(s) 248, 781, 923
Sse9I AATT 1 cut(s) 281
SsiI CCGC 6 cut(s) 38, 264, 333, 631, 671, 690
SspMI CTAG 3 cut(s) 338, 810, 821
StyD4I CCNGG 2 cut(s) 305, 768
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 5 cut(s) 103, 471, 617, 746, 1051
TaiI ACGT 5 cut(s) 186, 369, 513, 554, 962
TaqI TCGA 2 cut(s) 273, 974
TaqII GACCGA 1 cut(s) 302
TasI AATT 1 cut(s) 281
TatI WGTACW 3 cut(s) 564, 612, 858
TauI GCSGC 2 cut(s) 336, 693
TfiI GAWTC 2 cut(s) 95, 107
TscAI CASTG 3 cut(s) 58, 474, 690
TseFI GTSAC 1 cut(s) 724
TseI GCWGC 2 cut(s) 730, 1013
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 6 cut(s) 131, 417, 434, 625, 912, 989
TspGWI ACGGA 1 cut(s) 694
TspRI CASTG 3 cut(s) 58, 474, 690
VneI GTGCAC 1 cut(s) 940
VpaK11BI GGWCC 1 cut(s) 253
XceI RCATGY 1 cut(s) 859
XmiI GTMKAC 1 cut(s) 554
XspI CTAG 3 cut(s) 338, 810, 821
ZrmI AGTACT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.