Rw1G032420

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0013832
Physical Location & Seq
Forward (+)
60692375 .. 60696490
4116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032420.1

Sequence Viewer

Length: 1122 bp
ATGTTGGTTTGGAAATGTATTCAAAACCCTATTGTTCACACAGTCATGTTCACACTAGTCTTCTCTTTGGCCACCTTGATAATCCTACCGCCATCTCCGATCAGTGCCTCCTCTCCATCTGTTTCTGCTCTCTTCGCTTTCGGCGATTCCACGGTGGATTCCGGCATGAACAACGACCTCATGCCTTTCGTTCGGTGCGACCATCCCCCTTACGGCAGAGACCTGCCGGAACACGGCCCCACCGGAAGGTACTCCAATGGAAAGATTGCAACTGACTTTATGGCCTCAAACTTGGGACTCAAGGACCTCTTGCCTGCTTACCTCGACCCGAAACTGACCGACTATGATTTGCTCACCGGGGTCAGTTTCGCGTCGGGAACTTGCGGCCTAGATGATCTGACCTTGGAGTTGAGCCACGTCTATAGTATGGGGGATCAGTTGGAGTATTTTGATGAAGCCGTAGTGAGGATGAAGATAAGTGTTGGGGAAAAGAAGGGTAGAGAGATTGTGGAAAATGCACTGTTTGTGGTTAGTGTTGGGGCGAATGACTTGATGCTGAACGTGTATGAGTTTCCTCTAACCGAGAGGAAAACTCAGTTTACGTCTACAGACCAGTACTCTGATTTTCTGCTCCAGAGACTTGGGTCCTTTATTCAGGGTCTGTACAGTAGGGGAGCACGGCGGTTCATGGTGGCGGGTCTTCCACCGATCGGATGCCTTCCGCTTCAACGGACACTGAGCAGCATCATCAATCTGCGCCCTCAGCGCCTGTGCGTCACGCAGCAAAACATAGACTGTCAGACCTACAACTCCAAGCTCCAGGCCCTTCTCTCAAGTTTGCAAACCACACTGCCACTCCCTAGTTCAAGGCTAGTCTATTTTGACATCTACAACCCCATCATGGACATGTACAACAACCCAGATAAATATGGGTTTGTGCATATACATGAAGGATGCTGTGGAACTGGGATGTTTGAGTTGGGGCCTCTGTGCACAGAGTTTTCGCGGACGTGCCCCGACGCGTCGAAGTATCTTTTCTGGGATGCAGTTCATCCTACTGAAGCAGCATTTTTCGTCATTGCTGACTATGCTCATAAGACTGTCTTGCAGTACCTTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

373

Amino Acids

41.54

Weight (kDa)

5.24

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 361 1.1e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40250
fragaria_vesca FvH4_7g24850 FvH4_7g24850
malus_domestica MD01G1156400.v1.1 MD07G1225100.v1.1
prunus_persica Prupe.2G253600_v2.0.a1
pyrus_communis pycom07g20380
rosa_chinensis RchiOBHm_Chr1g0370731
rosa_laevigata RLG00000027015
rosa_multiflora Rmu_co8466073.1_g000001 Rmu_sc0004550.1_g000013
rosa_rugosa Rorug01G0358700
rosa_samantha Rh1AG368400 Rh1BG331900 Rh1CG345800 Rh1DG363300
rosa_wichuraiana Rw1G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 231
AccI GTMKAC 1 cut(s) 605
AccII CGCG 3 cut(s) 371, 1006, 1022
AciI CCGC 6 cut(s) 89, 384, 682, 695, 722, 1006
AclWI GGATC 1 cut(s) 441
AcoI YGGCCR 1 cut(s) 69
AcuI CTGAAG 1 cut(s) 1080
AfaI GTAC 5 cut(s) 251, 617, 665, 911, 1112
AfiI CCNNNNNNNGG 6 cut(s) 212, 233, 246, 465, 710, 901
AflIII ACRYGT 3 cut(s) 561, 906, 1020
AgsI TTSAA 3 cut(s) 23, 728, 867
AhlI ACTAGT 1 cut(s) 55
AjiI CACGTC 2 cut(s) 418, 1011
AjnI CCWGG 1 cut(s) 819
AluBI AGCT 1 cut(s) 817
AluI AGCT 1 cut(s) 817
Alw21I GWGCWC 2 cut(s) 679, 995
Alw26I GTCTC 2 cut(s) 213, 631
Alw44I GTGCAC 1 cut(s) 991
AlwI GGATC 1 cut(s) 441
AlwNI CAGNNNCTG 2 cut(s) 661, 769
AoxI GGCC 6 cut(s) 69, 235, 282, 385, 822, 983
ApaLI GTGCAC 1 cut(s) 991
ApeKI GCWGC 3 cut(s) 741, 781, 1064
AspLEI GCGC 2 cut(s) 759, 768
AspS9I GGNCC 5 cut(s) 236, 304, 645, 823, 983
AsuC2I CCSGG 1 cut(s) 358
AsuHPI GGTGA 1 cut(s) 346
AvaII GGWCC 2 cut(s) 304, 645
BaeGI GKGCMC 2 cut(s) 995, 1016
BalI TGGCCA 1 cut(s) 71
BbsI GAAGAC 2 cut(s) 52, 692
Bbv12I GWGCWC 2 cut(s) 679, 995
BbvCI CCTCAGC 1 cut(s) 762
BbvI GCAGC 3 cut(s) 753, 793, 1076
BccI CCATC 4 cut(s) 100, 124, 210, 905
BceAI ACGGC 4 cut(s) 229, 250, 443, 695
BciT130I CCWGG 1 cut(s) 821
BcnI CCSGG 1 cut(s) 358
BcoDI GTCTC 2 cut(s) 213, 631
BcuI ACTAGT 1 cut(s) 55
BfaI CTAG 4 cut(s) 56, 389, 861, 872
BfmI CTRYAG 2 cut(s) 421, 606
BfoI RGCGCY 1 cut(s) 769
BfuAI ACCTGC 1 cut(s) 231
BisI GCNGC 4 cut(s) 385, 742, 782, 1065
BlsI GCNGC 4 cut(s) 386, 743, 783, 1066
BmcAI AGTACT 1 cut(s) 617
Bme1390I CCNGG 2 cut(s) 358, 821
Bme18I GGWCC 2 cut(s) 304, 645
BmgBI CACGTC 2 cut(s) 418, 1011
BmgT120I GGNCC 5 cut(s) 236, 304, 645, 823, 983
BmiI GGNNCC 3 cut(s) 238, 646, 984
BmrFI CCNGG 2 cut(s) 358, 821
BmrI ACTGGG 1 cut(s) 975
BmsI GCATC 5 cut(s) 543, 704, 753, 944, 1033
BmuI ACTGGG 1 cut(s) 975
BoxI GACNNNNGTC 1 cut(s) 643
BpiI GAAGAC 2 cut(s) 52, 692
BplI GAGNNNNNCTC 2 cut(s) 577, 609
BpmI CTGGAG 2 cut(s) 617, 803
Bpu10I CCTNAGC 1 cut(s) 762
BpuEI CTTGAG 2 cut(s) 284, 817
BpuMI CCSGG 1 cut(s) 358
BsaI GGTCTC 1 cut(s) 213
BsaJI CCNNGG 3 cut(s) 150, 357, 402
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 840, 870
Bsc4I CCNNNNNNNGG 6 cut(s) 212, 233, 246, 465, 710, 901
Bse1I ACTGG 2 cut(s) 613, 970
Bse3DI GCAATG 1 cut(s) 1077
BseBI CCWGG 1 cut(s) 821
BseDI CCNNGG 3 cut(s) 150, 357, 402
BseGI GGATG 7 cut(s) 202, 474, 719, 959, 975, 1048, 1051
BseLI CCNNNNNNNGG 6 cut(s) 212, 233, 246, 465, 710, 901
BseMI GCAATG 1 cut(s) 1077
BseMII CTCAG 3 cut(s) 608, 728, 776
BseNI ACTGG 2 cut(s) 613, 970
BseRI GAGGAG 1 cut(s) 100
BseSI GKGCMC 2 cut(s) 995, 1016
BseXI GCAGC 3 cut(s) 753, 793, 1076
Bsh1236I CGCG 3 cut(s) 371, 1006, 1022
Bsh1285I CGRYCG 1 cut(s) 711
BshFI GGCC 6 cut(s) 71, 237, 284, 387, 824, 985
BsiEI CGRYCG 1 cut(s) 711
BsiHKAI GWGCWC 2 cut(s) 679, 995
BsiSI CCGG 4 cut(s) 162, 227, 243, 357
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 6 cut(s) 212, 233, 246, 465, 710, 901
BsmAI GTCTC 2 cut(s) 213, 631
BsmFI GGGAC 1 cut(s) 309
BsnI GGCC 6 cut(s) 71, 237, 284, 387, 824, 985
Bso31I GGTCTC 1 cut(s) 213
Bsp1286I GDGCHC 3 cut(s) 679, 995, 1016
Bsp1407I TGTACA 2 cut(s) 663, 909
Bsp143I GATC 4 cut(s) 99, 394, 433, 708
BspACI CCGC 6 cut(s) 89, 384, 682, 695, 722, 1006
BspANI GGCC 6 cut(s) 71, 237, 284, 387, 824, 985
BspCNI CTCAG 3 cut(s) 607, 729, 775
BspFNI CGCG 3 cut(s) 371, 1006, 1022
BspLI GGNNCC 3 cut(s) 238, 646, 984
BspMI ACCTGC 1 cut(s) 231
BspPI GGATC 1 cut(s) 441
BspTNI GGTCTC 1 cut(s) 213
BsrDI GCAATG 1 cut(s) 1077
BsrGI TGTACA 2 cut(s) 663, 909
BsrI ACTGG 2 cut(s) 613, 970
BssECI CCNNGG 3 cut(s) 150, 357, 402
BssMI GATC 4 cut(s) 99, 394, 433, 708
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 821
Bst4CI ACNGT 6 cut(s) 43, 154, 522, 668, 797, 1102
Bst6I CTCTTC 1 cut(s) 137
BstAUI TGTACA 2 cut(s) 663, 909
BstC8I GCNNGC 1 cut(s) 315
BstDEI CTNAG 4 cut(s) 594, 737, 762, 1115
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 7 cut(s) 202, 474, 719, 959, 975, 1048, 1051
BstFNI CGCG 3 cut(s) 371, 1006, 1022
BstH2I RGCGCY 1 cut(s) 769
BstHHI GCGC 2 cut(s) 759, 768
BstKTI GATC 4 cut(s) 102, 397, 436, 711
BstMAI GTCTC 2 cut(s) 213, 631
BstMBI GATC 4 cut(s) 99, 394, 433, 708
BstMCI CGRYCG 1 cut(s) 711
BstMWI GCNNNNNNNGC 4 cut(s) 134, 763, 765, 1088
BstNI CCWGG 1 cut(s) 821
BstNSI RCATGY 1 cut(s) 910
BstPAI GACNNNNGTC 1 cut(s) 643
BstSCI CCNGG 2 cut(s) 356, 819
BstSFI CTRYAG 2 cut(s) 421, 606
BstSLI GKGCMC 2 cut(s) 995, 1016
BstUI CGCG 3 cut(s) 371, 1006, 1022
BstV1I GCAGC 3 cut(s) 753, 793, 1076
BstV2I GAAGAC 2 cut(s) 52, 692
BstXI CCANNNNNNTGG 1 cut(s) 641
BsuRI GGCC 6 cut(s) 71, 237, 284, 387, 824, 985
BtgI CCRYGG 1 cut(s) 150
BtrI CACGTC 2 cut(s) 418, 1011
BtsCI GGATG 7 cut(s) 202, 474, 719, 959, 975, 1048, 1051
BtsI GCAGTG 1 cut(s) 848
BtsIMutI CAGTG 4 cut(s) 109, 518, 734, 848
BveI ACCTGC 1 cut(s) 231
Cac8I GCNNGC 1 cut(s) 315
CaiI CAGNNNCTG 2 cut(s) 661, 769
CfoI GCGC 2 cut(s) 759, 768
Cfr13I GGNCC 5 cut(s) 236, 304, 645, 823, 983
CseI GACGC 4 cut(s) 360, 763, 1011, 1028
Csp6I GTAC 5 cut(s) 250, 616, 664, 910, 1111
CviAII CATG 7 cut(s) 46, 166, 181, 688, 901, 907, 947
CviQI GTAC 5 cut(s) 250, 616, 664, 910, 1111
DdeI CTNAG 4 cut(s) 594, 737, 762, 1115
DpnI GATC 4 cut(s) 101, 396, 435, 710
DpnII GATC 4 cut(s) 99, 394, 433, 708
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 402
Eco31I GGTCTC 1 cut(s) 213
Eco47I GGWCC 2 cut(s) 304, 645
Eco57I CTGAAG 1 cut(s) 1080
EcoO109I RGGNCCY 4 cut(s) 304, 645, 823, 983
EcoRII CCWGG 1 cut(s) 819
EcoT14I CCWWGG 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 7 cut(s) 49, 169, 184, 691, 904, 910, 950
FalI AAGNNNNNCTT 4 cut(s) 293, 325, 1088, 1120
FaqI GGGAC 1 cut(s) 309
FatI CATG 7 cut(s) 45, 165, 180, 687, 900, 906, 946
FauI CCCGC 1 cut(s) 688
FblI GTMKAC 1 cut(s) 605
Fnu4HI GCNGC 4 cut(s) 385, 742, 782, 1065
FokI GGATG 7 cut(s) 189, 481, 726, 966, 982, 1038, 1055
Fsp4HI GCNGC 4 cut(s) 385, 742, 782, 1065
FspBI CTAG 4 cut(s) 56, 389, 861, 872
GlaI GCGC 2 cut(s) 758, 767
GluI GCNGC 4 cut(s) 385, 742, 782, 1065
GsuI CTGGAG 2 cut(s) 617, 803
HaeII RGCGCY 1 cut(s) 769
HaeIII GGCC 6 cut(s) 71, 237, 284, 387, 824, 985
HapII CCGG 4 cut(s) 162, 227, 243, 357
HgaI GACGC 4 cut(s) 360, 763, 1011, 1028
HhaI GCGC 2 cut(s) 759, 768
Hin1II CATG 7 cut(s) 49, 169, 184, 691, 904, 910, 950
Hin6I GCGC 2 cut(s) 757, 766
HinP1I GCGC 2 cut(s) 757, 766
HinfI GANTC 3 cut(s) 146, 158, 297
HpaII CCGG 4 cut(s) 162, 227, 243, 357
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 5 cut(s) 37, 51, 600, 606, 993
Hpy188I TCNGA 5 cut(s) 99, 399, 622, 713, 801
Hpy188III TCNNGA 2 cut(s) 375, 634
Hpy8I GTNNAC 5 cut(s) 37, 51, 600, 606, 993
Hpy99I CGWCG 3 cut(s) 376, 1022, 1027
HpyAV CCTTC 5 cut(s) 240, 487, 728, 836, 944
HpyCH4III ACNGT 6 cut(s) 43, 154, 522, 668, 797, 1102
HpyCH4IV ACGT 4 cut(s) 417, 561, 602, 1010
HpyCH4V TGCA 7 cut(s) 269, 518, 841, 940, 993, 1046, 1108
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 763, 765, 1088
HpyF3I CTNAG 4 cut(s) 594, 737, 762, 1115
HpySE526I ACGT 4 cut(s) 417, 561, 602, 1010
Hsp92II CATG 7 cut(s) 49, 169, 184, 691, 904, 910, 950
HspAI GCGC 2 cut(s) 757, 766
Kzo9I GATC 4 cut(s) 99, 394, 433, 708
LmnI GCTCC 3 cut(s) 636, 674, 822
Lsp1109I GCAGC 3 cut(s) 753, 793, 1076
LweI GCATC 5 cut(s) 543, 704, 753, 944, 1033
MaeI CTAG 4 cut(s) 56, 389, 861, 872
MaeII ACGT 4 cut(s) 417, 561, 602, 1010
MaeIII GTNAC 1 cut(s) 775
MalI GATC 4 cut(s) 101, 396, 435, 710
MboI GATC 4 cut(s) 99, 394, 433, 708
MboII GAAGA 4 cut(s) 52, 124, 484, 692
MhlI GDGCHC 3 cut(s) 679, 995, 1016
MlsI TGGCCA 1 cut(s) 71
MluI ACGCGT 1 cut(s) 1020
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 1 cut(s) 291
MmeI TCCRAC 1 cut(s) 420
Mox20I TGGCCA 1 cut(s) 71
MscI TGGCCA 1 cut(s) 71
MslI CAYNNNNRTG 3 cut(s) 44, 905, 945
Msp20I TGGCCA 1 cut(s) 71
MspI CCGG 4 cut(s) 162, 227, 243, 357
MspR9I CCNGG 2 cut(s) 358, 821
MvaI CCWGG 1 cut(s) 821
MvnI CGCG 3 cut(s) 371, 1006, 1022
MwoI GCNNNNNNNGC 4 cut(s) 134, 763, 765, 1088
NciI CCSGG 1 cut(s) 358
NdeII GATC 4 cut(s) 99, 394, 433, 708
NlaIII CATG 7 cut(s) 49, 169, 184, 691, 904, 910, 950
NlaIV GGNNCC 3 cut(s) 238, 646, 984
NmuCI GTSAC 1 cut(s) 775
NspI RCATGY 1 cut(s) 910
PciI ACATGT 1 cut(s) 906
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 195
PfeI GAWTC 2 cut(s) 146, 158
PkrI GCNGC 4 cut(s) 386, 743, 783, 1066
Ple19I CGATCG 1 cut(s) 711
PleI GAGTC 1 cut(s) 291
PpsI GAGTC 1 cut(s) 291
PpuMI RGGWCCY 2 cut(s) 304, 645
PscI ACATGT 1 cut(s) 906
PshAI GACNNNNGTC 1 cut(s) 643
Psp5II RGGWCCY 2 cut(s) 304, 645
Psp6I CCWGG 1 cut(s) 819
PspGI CCWGG 1 cut(s) 819
PspN4I GGNNCC 3 cut(s) 238, 646, 984
PspPI GGNCC 5 cut(s) 236, 304, 645, 823, 983
PspPPI RGGWCCY 2 cut(s) 304, 645
PstNI CAGNNNCTG 2 cut(s) 661, 769
PvuI CGATCG 1 cut(s) 711
RsaI GTAC 5 cut(s) 251, 617, 665, 911, 1112
RsaNI GTAC 5 cut(s) 250, 616, 664, 910, 1111
RseI CAYNNNNRTG 3 cut(s) 44, 905, 945
SatI GCNGC 4 cut(s) 385, 742, 782, 1065
Sau3AI GATC 4 cut(s) 99, 394, 433, 708
Sau96I GGNCC 5 cut(s) 236, 304, 645, 823, 983
ScaI AGTACT 1 cut(s) 617
SchI GAGTC 1 cut(s) 291
ScrFI CCNGG 2 cut(s) 358, 821
SduI GDGCHC 3 cut(s) 679, 995, 1016
SfaNI GCATC 5 cut(s) 543, 704, 753, 944, 1033
SfcI CTRYAG 2 cut(s) 421, 606
SinI GGWCC 2 cut(s) 304, 645
SmiMI CAYNNNNRTG 3 cut(s) 44, 905, 945
SmlI CTYRAG 2 cut(s) 299, 832
SmoI CTYRAG 2 cut(s) 299, 832
SpeI ACTAGT 1 cut(s) 55
SsiI CCGC 6 cut(s) 89, 384, 682, 695, 722, 1006
SspMI CTAG 4 cut(s) 56, 389, 861, 872
StyD4I CCNGG 2 cut(s) 356, 819
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 6 cut(s) 43, 154, 522, 668, 797, 1102
TaiI ACGT 4 cut(s) 420, 564, 605, 1013
TaqI TCGA 2 cut(s) 324, 1025
TaqII GACCGA 1 cut(s) 353
TatI WGTACW 3 cut(s) 615, 663, 909
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 2 cut(s) 146, 158
TscAI CASTG 4 cut(s) 109, 525, 741, 855
TseFI GTSAC 1 cut(s) 775
TseI GCWGC 3 cut(s) 741, 781, 1064
Tsp45I GTSAC 1 cut(s) 775
TspDTI ATGAA 6 cut(s) 182, 468, 485, 676, 963, 1040
TspGWI ACGGA 1 cut(s) 745
TspRI CASTG 4 cut(s) 109, 525, 741, 855
VneI GTGCAC 1 cut(s) 991
VpaK11BI GGWCC 2 cut(s) 304, 645
XceI RCATGY 1 cut(s) 910
XmiI GTMKAC 1 cut(s) 605
XspI CTAG 4 cut(s) 56, 389, 861, 872
ZrmI AGTACT 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.