RchiOBHm_Chr1g0370731

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0013832
Physical Location & Seq
Forward (+)
60180612 .. 60184772
4161 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59487

Sequence Viewer

Length: 1125 bp
ATGATGTTGGTTTGGAAATGTATTCAAAACCCTATTGTTCACACAGTCATGTTCACACTAGTCTTCTCTTTGGCCACCTTGATAATCCTACCGCCATCTCCGATCAGTGCCTCCTCTCCATCTGTTTCTGCTCTCTTCGCTTTCGGCGATTCCACGGTGGATTCCGGCATGAACAACGACCTCATGCCTTTCGTTCGGTGCGACCATCCCCCTTACGGCAGAGACCTGCCGGAACACGGCCCCACCGGAAGGTACTCCAATGGAAAGATTGCAACTGACTTTATGGCCTCAAACTTGGGACTCAAGGACCTCTTGCCTGCTTACCTCGACCCGAAACTGACCGACTATGATTTGCTCACCGGGGTCAGTTTCGCGTCGGGAACTTGCGGCCTAGATGATCTGACCTTGGAGTTGAGCCACGTCTATAGTATGGGGGATCAGTTGGAGTATTTTGATGAAGCCGTAGTGAGGATGAAGATAAGTGTTGGGGAAAAGAAGGGTAGAGAGATTGTGGAAAATGCACTGTTTGTGGTTAGTGTTGGGGCGAATGACTTGATGCTGAACGTGTATGAGTTTCCTCTAACCGAGAGGAAAACTCAGTTTACGTCTACAGACCAGTACTCTGATTTTCTGCTCCAGAGACTTGGGTCCTTTATTCAGGGTCTGTACAGTAGGGGAGCACGGCGGTTCATGGTGGCTGGTCTTCCACCGATCGGATGCCTTCCGCTTCAACGGACACTGAGCAGCATCATCAATCTGCGCCCTCAGCGCCTGTGCGTCACGCAGCAAAACATAGACTGTCAGACCTACAACTCCAAGCTCCAGGCCCTTCTCTCAAGTTTGCAAACCACACTGCCACTCCCTAGTTCAAGGCTAGTCTATTTTGACATCTACAACCCCATCATGGACATGTACAACAACCCAGATAAATATGGGTTTGTGCATATACATGAAGGATGCTGTGGAACTGGGATGTTTGAGTTGGGGCCTCTGTGCACAGAGTTTTCGCGGACGTGCCCCGACGCGTCGAAGTATCTTTTCTGGGATGCAGTTCATCCTACTGAAGCAGCATTTTTCGTCATTGCTGACTATGCTCATAAGACTGTCTTGCAGTACCTTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

374

Amino Acids

41.67

Weight (kDa)

5.24

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 362 1.1e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40250
fragaria_vesca FvH4_7g24850 FvH4_7g24850
malus_domestica MD01G1156400.v1.1 MD07G1225100.v1.1
prunus_persica Prupe.2G253600_v2.0.a1
pyrus_communis pycom07g20380
rosa_chinensis RchiOBHm_Chr1g0370731
rosa_laevigata RLG00000027015
rosa_multiflora Rmu_co8466073.1_g000001 Rmu_sc0004550.1_g000013
rosa_rugosa Rorug01G0358700
rosa_samantha Rh1AG368400 Rh1BG331900 Rh1CG345800 Rh1DG363300
rosa_wichuraiana Rw1G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 234
AccI GTMKAC 1 cut(s) 608
AccII CGCG 3 cut(s) 374, 1009, 1025
AciI CCGC 5 cut(s) 92, 387, 685, 725, 1009
AclWI GGATC 1 cut(s) 444
AcoI YGGCCR 1 cut(s) 72
AcuI CTGAAG 1 cut(s) 1083
AfaI GTAC 5 cut(s) 254, 620, 668, 914, 1115
AfiI CCNNNNNNNGG 6 cut(s) 215, 236, 249, 468, 713, 904
AflIII ACRYGT 3 cut(s) 564, 909, 1023
AgsI TTSAA 3 cut(s) 26, 731, 870
AhlI ACTAGT 1 cut(s) 58
AjiI CACGTC 2 cut(s) 421, 1014
AjnI CCWGG 1 cut(s) 822
AluBI AGCT 1 cut(s) 820
AluI AGCT 1 cut(s) 820
Alw21I GWGCWC 2 cut(s) 682, 998
Alw26I GTCTC 2 cut(s) 216, 634
Alw44I GTGCAC 1 cut(s) 994
AlwI GGATC 1 cut(s) 444
AlwNI CAGNNNCTG 2 cut(s) 664, 772
AoxI GGCC 6 cut(s) 72, 238, 285, 388, 825, 986
ApaLI GTGCAC 1 cut(s) 994
ApeKI GCWGC 3 cut(s) 744, 784, 1067
AspLEI GCGC 2 cut(s) 762, 771
AspS9I GGNCC 5 cut(s) 239, 307, 648, 826, 986
AsuC2I CCSGG 1 cut(s) 361
AsuHPI GGTGA 1 cut(s) 349
AvaII GGWCC 2 cut(s) 307, 648
BaeGI GKGCMC 2 cut(s) 998, 1019
BalI TGGCCA 1 cut(s) 74
BbsI GAAGAC 2 cut(s) 55, 695
Bbv12I GWGCWC 2 cut(s) 682, 998
BbvCI CCTCAGC 1 cut(s) 765
BbvI GCAGC 3 cut(s) 756, 796, 1079
BccI CCATC 4 cut(s) 103, 127, 213, 908
BceAI ACGGC 4 cut(s) 232, 253, 446, 698
BciT130I CCWGG 1 cut(s) 824
BcnI CCSGG 1 cut(s) 361
BcoDI GTCTC 2 cut(s) 216, 634
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 4 cut(s) 59, 392, 864, 875
BfmI CTRYAG 2 cut(s) 424, 609
BfoI RGCGCY 1 cut(s) 772
BfuAI ACCTGC 1 cut(s) 234
BisI GCNGC 4 cut(s) 388, 745, 785, 1068
BlsI GCNGC 4 cut(s) 389, 746, 786, 1069
BmcAI AGTACT 1 cut(s) 620
Bme1390I CCNGG 2 cut(s) 361, 824
Bme18I GGWCC 2 cut(s) 307, 648
BmgBI CACGTC 2 cut(s) 421, 1014
BmgT120I GGNCC 5 cut(s) 239, 307, 648, 826, 986
BmiI GGNNCC 3 cut(s) 241, 649, 987
BmrFI CCNGG 2 cut(s) 361, 824
BmrI ACTGGG 1 cut(s) 978
BmsI GCATC 5 cut(s) 546, 707, 756, 947, 1036
BmuI ACTGGG 1 cut(s) 978
BoxI GACNNNNGTC 1 cut(s) 646
BpiI GAAGAC 2 cut(s) 55, 695
BplI GAGNNNNNCTC 2 cut(s) 580, 612
BpmI CTGGAG 2 cut(s) 620, 806
Bpu10I CCTNAGC 1 cut(s) 765
BpuEI CTTGAG 2 cut(s) 287, 820
BpuMI CCSGG 1 cut(s) 361
BsaI GGTCTC 1 cut(s) 216
BsaJI CCNNGG 3 cut(s) 153, 360, 405
BsaWI WCCGGW 1 cut(s) 245
BsaXI ACNNNNNCTCC 2 cut(s) 843, 873
Bsc4I CCNNNNNNNGG 6 cut(s) 215, 236, 249, 468, 713, 904
Bse1I ACTGG 2 cut(s) 616, 973
Bse3DI GCAATG 1 cut(s) 1080
BseBI CCWGG 1 cut(s) 824
BseDI CCNNGG 3 cut(s) 153, 360, 405
BseGI GGATG 7 cut(s) 205, 477, 722, 962, 978, 1051, 1054
BseLI CCNNNNNNNGG 6 cut(s) 215, 236, 249, 468, 713, 904
BseMI GCAATG 1 cut(s) 1080
BseMII CTCAG 3 cut(s) 611, 731, 779
BseNI ACTGG 2 cut(s) 616, 973
BseRI GAGGAG 1 cut(s) 103
BseSI GKGCMC 2 cut(s) 998, 1019
BseXI GCAGC 3 cut(s) 756, 796, 1079
Bsh1236I CGCG 3 cut(s) 374, 1009, 1025
Bsh1285I CGRYCG 1 cut(s) 714
BshFI GGCC 6 cut(s) 74, 240, 287, 390, 827, 988
BsiEI CGRYCG 1 cut(s) 714
BsiHKAI GWGCWC 2 cut(s) 682, 998
BsiSI CCGG 4 cut(s) 165, 230, 246, 360
BslFI GGGAC 1 cut(s) 312
BslI CCNNNNNNNGG 6 cut(s) 215, 236, 249, 468, 713, 904
BsmAI GTCTC 2 cut(s) 216, 634
BsmFI GGGAC 1 cut(s) 312
BsnI GGCC 6 cut(s) 74, 240, 287, 390, 827, 988
Bso31I GGTCTC 1 cut(s) 216
Bsp1286I GDGCHC 3 cut(s) 682, 998, 1019
Bsp1407I TGTACA 2 cut(s) 666, 912
Bsp143I GATC 4 cut(s) 102, 397, 436, 711
BspACI CCGC 5 cut(s) 92, 387, 685, 725, 1009
BspANI GGCC 6 cut(s) 74, 240, 287, 390, 827, 988
BspCNI CTCAG 3 cut(s) 610, 732, 778
BspFNI CGCG 3 cut(s) 374, 1009, 1025
BspLI GGNNCC 3 cut(s) 241, 649, 987
BspMI ACCTGC 1 cut(s) 234
BspPI GGATC 1 cut(s) 444
BspTNI GGTCTC 1 cut(s) 216
BsrDI GCAATG 1 cut(s) 1080
BsrGI TGTACA 2 cut(s) 666, 912
BsrI ACTGG 2 cut(s) 616, 973
BssECI CCNNGG 3 cut(s) 153, 360, 405
BssMI GATC 4 cut(s) 102, 397, 436, 711
BssT1I CCWWGG 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 824
Bst4CI ACNGT 6 cut(s) 46, 157, 525, 671, 800, 1105
Bst6I CTCTTC 1 cut(s) 140
BstAUI TGTACA 2 cut(s) 666, 912
BstC8I GCNNGC 1 cut(s) 318
BstDEI CTNAG 4 cut(s) 597, 740, 765, 1118
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 7 cut(s) 205, 477, 722, 962, 978, 1051, 1054
BstFNI CGCG 3 cut(s) 374, 1009, 1025
BstH2I RGCGCY 1 cut(s) 772
BstHHI GCGC 2 cut(s) 762, 771
BstKTI GATC 4 cut(s) 105, 400, 439, 714
BstMAI GTCTC 2 cut(s) 216, 634
BstMBI GATC 4 cut(s) 102, 397, 436, 711
BstMCI CGRYCG 1 cut(s) 714
BstMWI GCNNNNNNNGC 4 cut(s) 137, 766, 768, 1091
BstNI CCWGG 1 cut(s) 824
BstNSI RCATGY 1 cut(s) 913
BstPAI GACNNNNGTC 1 cut(s) 646
BstSCI CCNGG 2 cut(s) 359, 822
BstSFI CTRYAG 2 cut(s) 424, 609
BstSLI GKGCMC 2 cut(s) 998, 1019
BstUI CGCG 3 cut(s) 374, 1009, 1025
BstV1I GCAGC 3 cut(s) 756, 796, 1079
BstV2I GAAGAC 2 cut(s) 55, 695
BstXI CCANNNNNNTGG 1 cut(s) 644
BsuRI GGCC 6 cut(s) 74, 240, 287, 390, 827, 988
BtgI CCRYGG 1 cut(s) 153
BtrI CACGTC 2 cut(s) 421, 1014
BtsCI GGATG 7 cut(s) 205, 477, 722, 962, 978, 1051, 1054
BtsI GCAGTG 1 cut(s) 851
BtsIMutI CAGTG 4 cut(s) 112, 521, 737, 851
BveI ACCTGC 1 cut(s) 234
Cac8I GCNNGC 1 cut(s) 318
CaiI CAGNNNCTG 2 cut(s) 664, 772
CfoI GCGC 2 cut(s) 762, 771
Cfr13I GGNCC 5 cut(s) 239, 307, 648, 826, 986
CseI GACGC 4 cut(s) 363, 766, 1014, 1031
Csp6I GTAC 5 cut(s) 253, 619, 667, 913, 1114
CviAII CATG 7 cut(s) 49, 169, 184, 691, 904, 910, 950
CviQI GTAC 5 cut(s) 253, 619, 667, 913, 1114
DdeI CTNAG 4 cut(s) 597, 740, 765, 1118
DpnI GATC 4 cut(s) 104, 399, 438, 713
DpnII GATC 4 cut(s) 102, 397, 436, 711
EaeI YGGCCR 1 cut(s) 72
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 405
Eco31I GGTCTC 1 cut(s) 216
Eco47I GGWCC 2 cut(s) 307, 648
Eco57I CTGAAG 1 cut(s) 1083
EcoO109I RGGNCCY 4 cut(s) 307, 648, 826, 986
EcoRII CCWGG 1 cut(s) 822
EcoT14I CCWWGG 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 7 cut(s) 52, 172, 187, 694, 907, 913, 953
FalI AAGNNNNNCTT 4 cut(s) 296, 328, 1091, 1123
FaqI GGGAC 1 cut(s) 312
FatI CATG 7 cut(s) 48, 168, 183, 690, 903, 909, 949
FblI GTMKAC 1 cut(s) 608
Fnu4HI GCNGC 4 cut(s) 388, 745, 785, 1068
FokI GGATG 7 cut(s) 192, 484, 729, 969, 985, 1041, 1058
Fsp4HI GCNGC 4 cut(s) 388, 745, 785, 1068
FspBI CTAG 4 cut(s) 59, 392, 864, 875
GlaI GCGC 2 cut(s) 761, 770
GluI GCNGC 4 cut(s) 388, 745, 785, 1068
GsuI CTGGAG 2 cut(s) 620, 806
HaeII RGCGCY 1 cut(s) 772
HaeIII GGCC 6 cut(s) 74, 240, 287, 390, 827, 988
HapII CCGG 4 cut(s) 165, 230, 246, 360
HgaI GACGC 4 cut(s) 363, 766, 1014, 1031
HhaI GCGC 2 cut(s) 762, 771
Hin1II CATG 7 cut(s) 52, 172, 187, 694, 907, 913, 953
Hin6I GCGC 2 cut(s) 760, 769
HinP1I GCGC 2 cut(s) 760, 769
HinfI GANTC 3 cut(s) 149, 161, 300
HpaII CCGG 4 cut(s) 165, 230, 246, 360
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 5 cut(s) 40, 54, 603, 609, 996
Hpy188I TCNGA 5 cut(s) 102, 402, 625, 716, 804
Hpy188III TCNNGA 2 cut(s) 378, 637
Hpy8I GTNNAC 5 cut(s) 40, 54, 603, 609, 996
Hpy99I CGWCG 3 cut(s) 379, 1025, 1030
HpyAV CCTTC 5 cut(s) 243, 490, 731, 839, 947
HpyCH4III ACNGT 6 cut(s) 46, 157, 525, 671, 800, 1105
HpyCH4IV ACGT 4 cut(s) 420, 564, 605, 1013
HpyCH4V TGCA 7 cut(s) 272, 521, 844, 943, 996, 1049, 1111
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 766, 768, 1091
HpyF3I CTNAG 4 cut(s) 597, 740, 765, 1118
HpySE526I ACGT 4 cut(s) 420, 564, 605, 1013
Hsp92II CATG 7 cut(s) 52, 172, 187, 694, 907, 913, 953
HspAI GCGC 2 cut(s) 760, 769
Kzo9I GATC 4 cut(s) 102, 397, 436, 711
LmnI GCTCC 3 cut(s) 639, 677, 825
Lsp1109I GCAGC 3 cut(s) 756, 796, 1079
LweI GCATC 5 cut(s) 546, 707, 756, 947, 1036
MaeI CTAG 4 cut(s) 59, 392, 864, 875
MaeII ACGT 4 cut(s) 420, 564, 605, 1013
MaeIII GTNAC 1 cut(s) 778
MalI GATC 4 cut(s) 104, 399, 438, 713
MboI GATC 4 cut(s) 102, 397, 436, 711
MboII GAAGA 4 cut(s) 55, 127, 487, 695
MhlI GDGCHC 3 cut(s) 682, 998, 1019
MlsI TGGCCA 1 cut(s) 74
MluI ACGCGT 1 cut(s) 1023
MluNI TGGCCA 1 cut(s) 74
MlyI GAGTC 1 cut(s) 294
MmeI TCCRAC 1 cut(s) 423
Mox20I TGGCCA 1 cut(s) 74
MscI TGGCCA 1 cut(s) 74
MslI CAYNNNNRTG 3 cut(s) 47, 908, 948
Msp20I TGGCCA 1 cut(s) 74
MspI CCGG 4 cut(s) 165, 230, 246, 360
MspR9I CCNGG 2 cut(s) 361, 824
MvaI CCWGG 1 cut(s) 824
MvnI CGCG 3 cut(s) 374, 1009, 1025
MwoI GCNNNNNNNGC 4 cut(s) 137, 766, 768, 1091
NciI CCSGG 1 cut(s) 361
NdeII GATC 4 cut(s) 102, 397, 436, 711
NlaIII CATG 7 cut(s) 52, 172, 187, 694, 907, 913, 953
NlaIV GGNNCC 3 cut(s) 241, 649, 987
NmuCI GTSAC 1 cut(s) 778
NspI RCATGY 1 cut(s) 913
PciI ACATGT 1 cut(s) 909
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 198
PfeI GAWTC 2 cut(s) 149, 161
PkrI GCNGC 4 cut(s) 389, 746, 786, 1069
Ple19I CGATCG 1 cut(s) 714
PleI GAGTC 1 cut(s) 294
PpsI GAGTC 1 cut(s) 294
PpuMI RGGWCCY 2 cut(s) 307, 648
PscI ACATGT 1 cut(s) 909
PshAI GACNNNNGTC 1 cut(s) 646
Psp5II RGGWCCY 2 cut(s) 307, 648
Psp6I CCWGG 1 cut(s) 822
PspGI CCWGG 1 cut(s) 822
PspN4I GGNNCC 3 cut(s) 241, 649, 987
PspPI GGNCC 5 cut(s) 239, 307, 648, 826, 986
PspPPI RGGWCCY 2 cut(s) 307, 648
PstNI CAGNNNCTG 2 cut(s) 664, 772
PvuI CGATCG 1 cut(s) 714
RsaI GTAC 5 cut(s) 254, 620, 668, 914, 1115
RsaNI GTAC 5 cut(s) 253, 619, 667, 913, 1114
RseI CAYNNNNRTG 3 cut(s) 47, 908, 948
SatI GCNGC 4 cut(s) 388, 745, 785, 1068
Sau3AI GATC 4 cut(s) 102, 397, 436, 711
Sau96I GGNCC 5 cut(s) 239, 307, 648, 826, 986
ScaI AGTACT 1 cut(s) 620
SchI GAGTC 1 cut(s) 294
ScrFI CCNGG 2 cut(s) 361, 824
SduI GDGCHC 3 cut(s) 682, 998, 1019
SfaNI GCATC 5 cut(s) 546, 707, 756, 947, 1036
SfcI CTRYAG 2 cut(s) 424, 609
SinI GGWCC 2 cut(s) 307, 648
SmiMI CAYNNNNRTG 3 cut(s) 47, 908, 948
SmlI CTYRAG 2 cut(s) 302, 835
SmoI CTYRAG 2 cut(s) 302, 835
SpeI ACTAGT 1 cut(s) 58
SsiI CCGC 5 cut(s) 92, 387, 685, 725, 1009
SspMI CTAG 4 cut(s) 59, 392, 864, 875
StyD4I CCNGG 2 cut(s) 359, 822
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 6 cut(s) 46, 157, 525, 671, 800, 1105
TaiI ACGT 4 cut(s) 423, 567, 608, 1016
TaqI TCGA 2 cut(s) 327, 1028
TaqII GACCGA 1 cut(s) 356
TatI WGTACW 3 cut(s) 618, 666, 912
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 2 cut(s) 149, 161
TscAI CASTG 4 cut(s) 112, 528, 744, 858
TseFI GTSAC 1 cut(s) 778
TseI GCWGC 3 cut(s) 744, 784, 1067
Tsp45I GTSAC 1 cut(s) 778
TspDTI ATGAA 6 cut(s) 185, 471, 488, 679, 966, 1043
TspGWI ACGGA 1 cut(s) 748
TspRI CASTG 4 cut(s) 112, 528, 744, 858
VneI GTGCAC 1 cut(s) 994
VpaK11BI GGWCC 2 cut(s) 307, 648
XceI RCATGY 1 cut(s) 913
XmiI GTMKAC 1 cut(s) 608
XspI CTAG 4 cut(s) 59, 392, 864, 875
ZrmI AGTACT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.