MD01G1156400.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013832
Physical Location & Seq
Forward (+)
26392275 .. 26393990
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1156400.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGAGCAAAAAACCACCGTCCTCTTTTTACTCTTATTCTCTTTGGCCTCCTTTCTATCTCTACTAATCCCAACCACCGCATCTCCGCCTGTATCCGCCGTTTTCGCCTTAGGCGATTCAACGTTGGATTCGGGCAACAACAACCGCCTCCCATTCACCCTTTTCAAAAGTGACCACCGTCCGTACGGCCGGGACTTGCCAAACCACGTCCCGACGGGAAGGTTTTCCAATGGAAAACTATCAACGGACTTCATGGTCTCCAAACTTGGCCTCAAGGACTTGTTGACCGCTTACCGTGACCGGAGACTGACTGACCGTGACTTGCTCACCGGTGTCAGTTTCGCGTCAGGTGGTAGCGGTTTGGATGATCTGACCTTGGCCGTGTCGAAGGCTACGAGTTTGTCGAGGCAGTTGGAGGACTTTGATGAAGCGTTGAGGAGGATGAGGAGAACTGTTGGGGAGAAGAATTGTAGTGAGATTGTCAACAACGCTGTGTTTGTGATTAGTGTTGGGACTAATGACATGGTGTTTAACCTCTACGATGTACCTGTTACACGGAGGAAAATTGAGTTTTCACCTTCAGGGTACCAGGATTTTCTTCTCAAAAGACTCGGGTCGTTCATTCAGAACCTACATAAGAGGGGAGCGAGGAGATTCCAGGTGGCGGGTCTTCCGCCAATTGGATGTCTGCCAATGCAGCTGACCATTGGCAGCATCTTCAATGGGCTTCGGCGTGTGTGCGTGGAGCAGCAAAACAAGGACTCTCAGGCTTACAACACCAACCTCCAAGGCCTCATCCAAAGATTGCAAGCCTCCCTCCCCGGTTCCAGGCTCGCCTACTTCGACACCTACCATCCCATCTTGGACATGTTCAATAACCCATCTAAATATGGGTTTGTGCAAACACATGCAGGATGCTGTGGAACAGGGATGGTAGAGTTGGGTCCAATGTGCGGGAAGCTTTCACTGACGTGTCCAGACCCTTCTAAATTTTTGTTCTGGGATGCCATGCATCCTTCCCAAGCAGCATACAAAGTTCTTGCTGACATATCAGAGAAGACGGTCTTGCCGTACCTTACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

362

Amino Acids

39.94

Weight (kDa)

9.12

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 349 4.9e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40250
fragaria_vesca FvH4_7g24850 FvH4_7g24850
malus_domestica MD01G1156400.v1.1 MD07G1225100.v1.1
prunus_persica Prupe.2G253600_v2.0.a1
pyrus_communis pycom07g20380
rosa_chinensis RchiOBHm_Chr1g0370731
rosa_laevigata RLG00000027015
rosa_multiflora Rmu_co8466073.1_g000001 Rmu_sc0004550.1_g000013
rosa_rugosa Rorug01G0358700
rosa_samantha Rh1AG368400 Rh1BG331900 Rh1CG345800 Rh1DG363300
rosa_wichuraiana Rw1G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 254
Acc65I GGTACC 1 cut(s) 585
AccB1I GGYRCC 1 cut(s) 585
AccII CGCG 1 cut(s) 344
AciI CCGC 9 cut(s) 78, 86, 96, 145, 288, 357, 665, 674, 954
AclI AACGTT 1 cut(s) 122
AcoI YGGCCR 2 cut(s) 187, 378
AcsI RAATTY 1 cut(s) 989
AcuI CTGAAG 1 cut(s) 564
AfaI GTAC 4 cut(s) 185, 546, 587, 1073
AfiI CCNNNNNNNGG 4 cut(s) 664, 680, 828, 953
AflIII ACRYGT 2 cut(s) 867, 971
AgeI ACCGGT 1 cut(s) 329
AgsI TTSAA 4 cut(s) 120, 166, 721, 874
AjiI CACGTC 2 cut(s) 208, 972
AjnI CCWGG 3 cut(s) 588, 657, 827
AleI CACNNNNGTG 1 cut(s) 970
AluBI AGCT 2 cut(s) 700, 961
AluI AGCT 2 cut(s) 700, 961
Alw26I GTCTC 2 cut(s) 262, 298
Ama87I CYCGRG 1 cut(s) 611
AoxI GGCC 5 cut(s) 45, 187, 268, 378, 790
ApeKI GCWGC 4 cut(s) 697, 711, 748, 1025
ApoI RAATTY 1 cut(s) 989
AsiGI ACCGGT 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 223
Asp718I GGTACC 1 cut(s) 585
AspS9I GGNCC 1 cut(s) 944
AsuC2I CCSGG 2 cut(s) 191, 822
AsuHPI GGTGA 3 cut(s) 148, 319, 567
AvaI CYCGRG 1 cut(s) 611
AvaII GGWCC 1 cut(s) 944
AxyI CCTNAGG 1 cut(s) 109
BanI GGYRCC 1 cut(s) 585
BbsI GAAGAC 2 cut(s) 662, 1064
BbvI GCAGC 4 cut(s) 709, 723, 760, 1037
BccI CCATC 4 cut(s) 861, 866, 889, 925
BceAI ACGGC 4 cut(s) 83, 202, 365, 1054
BciT130I CCWGG 3 cut(s) 590, 659, 829
BciVI GTATCC 1 cut(s) 103
BcnI CCSGG 2 cut(s) 191, 822
BcoDI GTCTC 2 cut(s) 262, 298
BfuI GTATCC 1 cut(s) 103
BisI GCNGC 4 cut(s) 698, 712, 749, 1026
BlsI GCNGC 4 cut(s) 699, 713, 750, 1027
Bme1390I CCNGG 5 cut(s) 191, 590, 659, 822, 829
Bme18I GGWCC 1 cut(s) 944
BmeT110I CYCGRG 1 cut(s) 611
BmgBI CACGTC 2 cut(s) 208, 972
BmgT120I GGNCC 1 cut(s) 944
BmiI GGNNCC 3 cut(s) 587, 826, 945
BmrFI CCNGG 5 cut(s) 191, 590, 659, 822, 829
BmsI GCATC 5 cut(s) 89, 723, 905, 994, 1021
BoxI GACNNNNGTC 2 cut(s) 177, 613
BpiI GAAGAC 2 cut(s) 662, 1064
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 2 cut(s) 191, 822
BsaI GGTCTC 1 cut(s) 262
BsaJI CCNNGG 3 cut(s) 375, 787, 820
BsaWI WCCGGW 2 cut(s) 300, 329
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 4 cut(s) 664, 680, 828, 953
Bse118I RCCGGY 1 cut(s) 329
Bse21I CCTNAGG 1 cut(s) 109
BseBI CCWGG 3 cut(s) 590, 659, 829
BseDI CCNNGG 3 cut(s) 375, 787, 820
BseGI GGATG 9 cut(s) 370, 447, 689, 795, 853, 920, 936, 1009, 1012
BseLI CCNNNNNNNGG 4 cut(s) 664, 680, 828, 953
BseMII CTCAG 1 cut(s) 779
BseRI GAGGAG 3 cut(s) 451, 460, 664
BseX3I CGGCCG 1 cut(s) 187
BseXI GCAGC 4 cut(s) 709, 723, 760, 1037
Bsh1236I CGCG 1 cut(s) 344
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 5 cut(s) 47, 189, 270, 380, 792
BshNI GGYRCC 1 cut(s) 585
BshTI ACCGGT 1 cut(s) 329
BsiEI CGRYCG 1 cut(s) 190
BsiHKCI CYCGRG 1 cut(s) 611
BsiSI CCGG 4 cut(s) 190, 301, 330, 822
BsiWI CGTACG 1 cut(s) 183
BslFI GGGAC 3 cut(s) 194, 206, 526
BslI CCNNNNNNNGG 4 cut(s) 664, 680, 828, 953
BsmAI GTCTC 2 cut(s) 262, 298
BsmFI GGGAC 3 cut(s) 194, 206, 526
BsnI GGCC 5 cut(s) 47, 189, 270, 380, 792
Bso31I GGTCTC 1 cut(s) 262
BsoBI CYCGRG 1 cut(s) 611
Bsp143I GATC 1 cut(s) 367
BspACI CCGC 9 cut(s) 78, 86, 96, 145, 288, 357, 665, 674, 954
BspANI GGCC 5 cut(s) 47, 189, 270, 380, 792
BspCNI CTCAG 1 cut(s) 778
BspFNI CGCG 1 cut(s) 344
BspLI GGNNCC 3 cut(s) 587, 826, 945
BspT107I GGYRCC 1 cut(s) 585
BspTNI GGTCTC 1 cut(s) 262
BsrFI RCCGGY 1 cut(s) 329
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 3 cut(s) 375, 787, 820
BssMI GATC 1 cut(s) 367
BssT1I CCWWGG 2 cut(s) 375, 787
Bst2UI CCWGG 3 cut(s) 590, 659, 829
Bst4CI ACNGT 6 cut(s) 19, 179, 296, 317, 454, 1063
BstC8I GCNNGC 2 cut(s) 810, 834
BstDEI CTNAG 2 cut(s) 109, 765
BstF5I GGATG 9 cut(s) 370, 447, 689, 795, 853, 920, 936, 1009, 1012
BstFNI CGCG 1 cut(s) 344
BstKTI GATC 1 cut(s) 370
BstMAI GTCTC 2 cut(s) 262, 298
BstMBI GATC 1 cut(s) 367
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 2 cut(s) 104, 697
BstNI CCWGG 3 cut(s) 590, 659, 829
BstNSI RCATGY 2 cut(s) 871, 911
BstPAI GACNNNNGTC 2 cut(s) 177, 613
BstSCI CCNGG 5 cut(s) 189, 588, 657, 820, 827
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 4 cut(s) 709, 723, 760, 1037
BstV2I GAAGAC 2 cut(s) 662, 1064
BstZI CGGCCG 1 cut(s) 187
Bsu36I CCTNAGG 1 cut(s) 109
BsuI GTATCC 1 cut(s) 103
BsuRI GGCC 5 cut(s) 47, 189, 270, 380, 792
BtrI CACGTC 2 cut(s) 208, 972
BtsCI GGATG 9 cut(s) 370, 447, 689, 795, 853, 920, 936, 1009, 1012
BtsIMutI CAGTG 1 cut(s) 965
Cac8I GCNNGC 2 cut(s) 810, 834
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 1 cut(s) 944
CseI GACGC 1 cut(s) 333
Csp6I GTAC 4 cut(s) 184, 545, 586, 1072
CspAI ACCGGT 1 cut(s) 329
CviAII CATG 5 cut(s) 253, 523, 868, 908, 1009
CviQI GTAC 4 cut(s) 184, 545, 586, 1072
DdeI CTNAG 2 cut(s) 109, 765
DpnI GATC 1 cut(s) 369
DpnII GATC 1 cut(s) 367
DrdI GACNNNNNNGTC 1 cut(s) 254
DseDI GACNNNNNNGTC 1 cut(s) 254
EaeI YGGCCR 2 cut(s) 187, 378
EagI CGGCCG 1 cut(s) 187
EciI GGCGGA 3 cut(s) 75, 85, 663
EclXI CGGCCG 1 cut(s) 187
Eco130I CCWWGG 2 cut(s) 375, 787
Eco147I AGGCCT 1 cut(s) 792
Eco31I GGTCTC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 944
Eco52I CGGCCG 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 564
Eco81I CCTNAGG 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 611
EcoRII CCWGG 3 cut(s) 588, 657, 827
EcoT14I CCWWGG 2 cut(s) 375, 787
EcoT22I ATGCAT 1 cut(s) 1014
ErhI CCWWGG 2 cut(s) 375, 787
FaeI CATG 5 cut(s) 256, 526, 871, 911, 1012
FaiI YATR 9 cut(s) 254, 524, 636, 869, 891, 909, 1010, 1030, 1049
FalI AAGNNNNNCTT 2 cut(s) 1049, 1081
FaqI GGGAC 3 cut(s) 194, 206, 526
FatI CATG 5 cut(s) 252, 522, 867, 907, 1008
FauI CCCGC 2 cut(s) 658, 947
Fnu4HI GCNGC 4 cut(s) 698, 712, 749, 1026
FokI GGATG 9 cut(s) 377, 454, 696, 782, 840, 927, 943, 999, 1016
Fsp4HI GCNGC 4 cut(s) 698, 712, 749, 1026
GluI GCNGC 4 cut(s) 698, 712, 749, 1026
HaeIII GGCC 5 cut(s) 47, 189, 270, 380, 792
HapII CCGG 4 cut(s) 190, 301, 330, 822
HgaI GACGC 1 cut(s) 333
Hin1II CATG 5 cut(s) 256, 526, 871, 911, 1012
HincII GTYRAC 2 cut(s) 285, 484
HindII GTYRAC 2 cut(s) 285, 484
HindIII AAGCTT 1 cut(s) 959
HinfI GANTC 5 cut(s) 116, 128, 609, 654, 761
HpaII CCGG 4 cut(s) 190, 301, 330, 822
HphI GGTGA 3 cut(s) 148, 319, 567
Hpy166II GTNNAC 2 cut(s) 285, 484
Hpy188I TCNGA 3 cut(s) 372, 627, 1054
Hpy188III TCNNGA 2 cut(s) 211, 977
Hpy8I GTNNAC 2 cut(s) 285, 484
Hpy99I CGWCG 1 cut(s) 217
HpyAV CCTTC 5 cut(s) 213, 382, 588, 993, 1026
HpyCH4III ACNGT 6 cut(s) 19, 179, 296, 317, 454, 1063
HpyCH4IV ACGT 3 cut(s) 122, 207, 971
HpyCH4V TGCA 5 cut(s) 697, 808, 901, 911, 1012
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 697
HpyF3I CTNAG 2 cut(s) 109, 765
HpySE526I ACGT 3 cut(s) 122, 207, 971
Hsp92II CATG 5 cut(s) 256, 526, 871, 911, 1012
KpnI GGTACC 1 cut(s) 589
Kzo9I GATC 1 cut(s) 367
LmnI GCTCC 3 cut(s) 4, 644, 745
Lsp1109I GCAGC 4 cut(s) 709, 723, 760, 1037
LweI GCATC 5 cut(s) 89, 723, 905, 994, 1021
MaeII ACGT 3 cut(s) 122, 207, 971
MaeIII GTNAC 4 cut(s) 170, 296, 317, 550
MalI GATC 1 cut(s) 369
MboI GATC 1 cut(s) 367
MboII GAAGA 5 cut(s) 475, 590, 662, 709, 1069
MfeI CAATTG 1 cut(s) 678
MluCI AATT 4 cut(s) 466, 564, 678, 989
MlyI GAGTC 2 cut(s) 603, 755
MmeI TCCRAC 2 cut(s) 105, 393
Mph1103I ATGCAT 1 cut(s) 1014
MroXI GAANNNNTTC 1 cut(s) 223
MseI TTAA 1 cut(s) 531
MslI CAYNNNNRTG 1 cut(s) 970
MspA1I CMGCKG 1 cut(s) 700
MspI CCGG 4 cut(s) 190, 301, 330, 822
MspR9I CCNGG 5 cut(s) 191, 590, 659, 822, 829
MunI CAATTG 1 cut(s) 678
MvaI CCWGG 3 cut(s) 590, 659, 829
MvnI CGCG 1 cut(s) 344
MwoI GCNNNNNNNGC 2 cut(s) 104, 697
NciI CCSGG 2 cut(s) 191, 822
NdeII GATC 1 cut(s) 367
NlaIII CATG 5 cut(s) 256, 526, 871, 911, 1012
NlaIV GGNNCC 3 cut(s) 587, 826, 945
NmuCI GTSAC 3 cut(s) 170, 296, 317
NsiI ATGCAT 1 cut(s) 1014
NspI RCATGY 2 cut(s) 871, 911
OliI CACNNNNGTG 1 cut(s) 970
PceI AGGCCT 1 cut(s) 792
PciI ACATGT 1 cut(s) 867
PcsI WCGNNNNNNNCGW 5 cut(s) 111, 392, 401, 840, 1067
PdmI GAANNNNTTC 1 cut(s) 223
PfeI GAWTC 3 cut(s) 116, 128, 654
Pfl23II CGTACG 1 cut(s) 183
PinAI ACCGGT 1 cut(s) 329
PkrI GCNGC 4 cut(s) 699, 713, 750, 1027
PleI GAGTC 2 cut(s) 603, 755
PpsI GAGTC 2 cut(s) 603, 755
PscI ACATGT 1 cut(s) 867
PshAI GACNNNNGTC 2 cut(s) 177, 613
Psp1406I AACGTT 1 cut(s) 122
Psp6I CCWGG 3 cut(s) 588, 657, 827
PspGI CCWGG 3 cut(s) 588, 657, 827
PspLI CGTACG 1 cut(s) 183
PspN4I GGNNCC 3 cut(s) 587, 826, 945
PspPI GGNCC 1 cut(s) 944
PvuII CAGCTG 1 cut(s) 700
RsaI GTAC 4 cut(s) 185, 546, 587, 1073
RsaNI GTAC 4 cut(s) 184, 545, 586, 1072
RseI CAYNNNNRTG 1 cut(s) 970
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 4 cut(s) 698, 712, 749, 1026
Sau3AI GATC 1 cut(s) 367
Sau96I GGNCC 1 cut(s) 944
SchI GAGTC 2 cut(s) 603, 755
ScrFI CCNGG 5 cut(s) 191, 590, 659, 822, 829
SfaNI GCATC 5 cut(s) 89, 723, 905, 994, 1021
SgrAI CRCCGGYG 1 cut(s) 329
SinI GGWCC 1 cut(s) 944
SmiMI CAYNNNNRTG 1 cut(s) 970
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 4 cut(s) 466, 564, 678, 989
SseBI AGGCCT 1 cut(s) 792
SsiI CCGC 9 cut(s) 78, 86, 96, 145, 288, 357, 665, 674, 954
StuI AGGCCT 1 cut(s) 792
StyD4I CCNGG 5 cut(s) 189, 588, 657, 820, 827
StyI CCWWGG 2 cut(s) 375, 787
TaaI ACNGT 6 cut(s) 19, 179, 296, 317, 454, 1063
TaiI ACGT 3 cut(s) 125, 210, 974
TaqI TCGA 3 cut(s) 386, 404, 843
TasI AATT 4 cut(s) 466, 564, 678, 989
TfiI GAWTC 3 cut(s) 116, 128, 654
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 1 cut(s) 972
TseFI GTSAC 3 cut(s) 170, 296, 317
TseI GCWGC 4 cut(s) 697, 711, 748, 1025
Tsp45I GTSAC 3 cut(s) 170, 296, 317
TspDTI ATGAA 3 cut(s) 241, 441, 610
TspGWI ACGGA 3 cut(s) 171, 260, 571
TspRI CASTG 1 cut(s) 972
VpaK11BI GGWCC 1 cut(s) 944
XapI RAATTY 1 cut(s) 989
XceI RCATGY 2 cut(s) 871, 911
XmnI GAANNNNTTC 1 cut(s) 223
Zsp2I ATGCAT 1 cut(s) 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.