FvH4_7g24850

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013832
Physical Location & Seq
Forward (+)
19102009 .. 19103890
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24850.t2

Sequence Viewer

Length: 1101 bp
ATGATGTTGGTCACAGTACTAGTCTTCTGTTTCGCCACCTTGATAATCCTACCGCCATCTCCGACGACCTGCACTGCCTCCTCCTCCTCCTCTCCATCTGTTTCTGCTCTTTTCGCTTTCGGCGATTCCACGGTGGACACCGGCATGAACAACGGCCTTTCTATGCCTTTATATAAGTGCAACCATCCCCCTTACGGCAAAGACCTACCGGGGCAAGTCCCCACCGGAAGGTACTCCAATGGAAAGATTGGAGTTGACTTTATGGCCTCAAACTTGGGACTCAAGGAAGACCTTTTGCCCTCTTACCTCGACCCGAAACTGACAGACCATGATTTGCTCACCGGGGTCAGCTTCGCGTCGGGAGGTTGTGGGCTAGATGATCTGACCTTGCAGTTAACCCAAGTCTATAGCATGCAGGATCAGTTGGAGTATTTTGATGAAGCCGTAGAAAGGATTAAGACAAGTGTTGGGGAGAAGAATGGTAGCGAGATTGTGGAAAATGCACTGTTTGTGGTTGGTGTTGGGACGAATGACCTCATCCTGAACGTGTATGACTTTCCTCTAACGCAGAGGAAATCTCAGTTTACATCTACAGACCAGTACTTTGATTTTCTGCTCCAAAAACTCGGGTCTTTTATTCAGGATCTGTACAGTAGGGGAGCAAGGCGGTTCATGGTGGCGGGTTTTCCACCGATCGGATGCCTTCCGCTTCAACAGACATTAGGCGGCATCATCAATCTGCGTCGGCGCGTGTGCATCACACAACAAAACATAGACTGTCAGACCTACAACTCCAAGCTCCAAGCCCTTCTCTCAAGTTTGCAAACCACATTACTCCCTAGTTCAAGGCTGCTCTACTATGACATCTACAACCCCATGATGGACATGTTCAACAACCCAGATAAATATGGGTTTGTGCAAGTAAGTGAAGGATGCTGCGGAACTGGGCTGGTTGAGTTGGGGCCTCTGTGCTCACGGCTCTCGCGGACGTGCCCCGACGCGTCAAAGTATATGTTCTGGGATACGGTTCATCCTACTGAAGTAGCATTAGCTGTTGTTGTAGACTATAATCAAAAGACTGTTTTGCAGTTCCTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

367

Amino Acids

40.34

Weight (kDa)

4.85

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 294 1e-19 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40250
fragaria_vesca FvH4_7g24850 FvH4_7g24850
malus_domestica MD01G1156400.v1.1 MD07G1225100.v1.1
prunus_persica Prupe.2G253600_v2.0.a1
pyrus_communis pycom07g20380
rosa_chinensis RchiOBHm_Chr1g0370731
rosa_laevigata RLG00000027015
rosa_multiflora Rmu_co8466073.1_g000001 Rmu_sc0004550.1_g000013
rosa_rugosa Rorug01G0358700
rosa_samantha Rh1AG368400 Rh1BG331900 Rh1CG345800 Rh1DG363300
rosa_wichuraiana Rw1G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 77
AccI GTMKAC 1 cut(s) 1062
AccII CGCG 4 cut(s) 356, 750, 985, 1001
AciI CCGC 7 cut(s) 53, 667, 680, 707, 726, 939, 985
AclWI GGATC 2 cut(s) 426, 651
AcuI CTGAAG 1 cut(s) 1059
AfaI GTAC 4 cut(s) 18, 233, 602, 650
AfiI CCNNNNNNNGG 5 cut(s) 194, 228, 450, 695, 880
AflIII ACRYGT 3 cut(s) 546, 885, 999
AgsI TTSAA 3 cut(s) 713, 846, 892
AhlI ACTAGT 1 cut(s) 19
AjiI CACGTC 1 cut(s) 990
AluBI AGCT 3 cut(s) 351, 799, 1052
AluI AGCT 3 cut(s) 351, 799, 1052
Alw21I GWGCWC 1 cut(s) 974
AlwI GGATC 2 cut(s) 426, 651
AlwNI CAGNNNCTG 1 cut(s) 646
Ama87I CYCGRG 1 cut(s) 626
AoxI GGCC 3 cut(s) 154, 264, 962
ApeKI GCWGC 2 cut(s) 850, 936
ArsI GACNNNNNNTTYG 2 cut(s) 587, 619
AspLEI GCGC 1 cut(s) 750
AspS9I GGNCC 1 cut(s) 962
AsuC2I CCSGG 2 cut(s) 210, 343
AsuHPI GGTGA 1 cut(s) 331
AvaI CYCGRG 1 cut(s) 626
BaeGI GKGCMC 1 cut(s) 995
BbsI GAAGAC 2 cut(s) 16, 294
Bbv12I GWGCWC 1 cut(s) 974
BbvI GCAGC 2 cut(s) 837, 923
BccI CCATC 4 cut(s) 64, 103, 192, 874
BceAI ACGGC 4 cut(s) 169, 211, 428, 992
BciVI GTATCC 1 cut(s) 1015
BcnI CCSGG 2 cut(s) 210, 343
BcuI ACTAGT 1 cut(s) 19
BfaI CTAG 3 cut(s) 20, 374, 840
BfmI CTRYAG 2 cut(s) 406, 591
BfuAI ACCTGC 1 cut(s) 77
BfuI GTATCC 1 cut(s) 1015
BisI GCNGC 3 cut(s) 727, 851, 937
BlsI GCNGC 3 cut(s) 728, 852, 938
BmcAI AGTACT 2 cut(s) 18, 602
Bme1390I CCNGG 2 cut(s) 210, 343
BmeT110I CYCGRG 1 cut(s) 626
BmgBI CACGTC 1 cut(s) 990
BmgT120I GGNCC 1 cut(s) 962
BmiI GGNNCC 1 cut(s) 963
BmrFI CCNGG 2 cut(s) 210, 343
BmrI ACTGGG 1 cut(s) 954
BmsI GCATC 4 cut(s) 689, 738, 765, 923
BmuI ACTGGG 1 cut(s) 954
BpiI GAAGAC 2 cut(s) 16, 294
BplI GAGNNNNNCTC 2 cut(s) 562, 594
BpuEI CTTGAG 2 cut(s) 266, 799
BpuMI CCSGG 2 cut(s) 210, 343
BsaJI CCNNGG 3 cut(s) 129, 209, 342
BsaWI WCCGGW 1 cut(s) 224
Bsc4I CCNNNNNNNGG 5 cut(s) 194, 228, 450, 695, 880
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 2 cut(s) 598, 949
BseDI CCNNGG 3 cut(s) 129, 209, 342
BseGI GGATG 5 cut(s) 184, 537, 704, 938, 1030
BseLI CCNNNNNNNGG 5 cut(s) 194, 228, 450, 695, 880
BseMII CTCAG 1 cut(s) 593
BseNI ACTGG 2 cut(s) 598, 949
BseRI GAGGAG 4 cut(s) 70, 73, 76, 79
BseSI GKGCMC 1 cut(s) 995
BseXI GCAGC 2 cut(s) 837, 923
BsgI GTGCAG 1 cut(s) 55
Bsh1236I CGCG 4 cut(s) 356, 750, 985, 1001
Bsh1285I CGRYCG 1 cut(s) 696
BshFI GGCC 3 cut(s) 156, 266, 964
BsiEI CGRYCG 1 cut(s) 696
BsiHKAI GWGCWC 1 cut(s) 974
BsiHKCI CYCGRG 1 cut(s) 626
BsiSI CCGG 4 cut(s) 141, 209, 225, 342
BslFI GGGAC 3 cut(s) 203, 291, 538
BslI CCNNNNNNNGG 5 cut(s) 194, 228, 450, 695, 880
BsmFI GGGAC 3 cut(s) 203, 291, 538
BsnI GGCC 3 cut(s) 156, 266, 964
BsoBI CYCGRG 1 cut(s) 626
Bsp1286I GDGCHC 2 cut(s) 974, 995
Bsp1407I TGTACA 1 cut(s) 648
Bsp143I GATC 4 cut(s) 379, 418, 643, 693
BspACI CCGC 7 cut(s) 53, 667, 680, 707, 726, 939, 985
BspANI GGCC 3 cut(s) 156, 266, 964
BspCNI CTCAG 1 cut(s) 592
BspFNI CGCG 4 cut(s) 356, 750, 985, 1001
BspLI GGNNCC 1 cut(s) 963
BspMI ACCTGC 1 cut(s) 77
BspPI GGATC 2 cut(s) 426, 651
BsrFI RCCGGY 1 cut(s) 140
BsrGI TGTACA 1 cut(s) 648
BsrI ACTGG 2 cut(s) 598, 949
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 3 cut(s) 129, 209, 342
BssMI GATC 4 cut(s) 379, 418, 643, 693
Bst4CI ACNGT 7 cut(s) 16, 133, 507, 653, 779, 1027, 1081
BstAUI TGTACA 1 cut(s) 648
BstC8I GCNNGC 1 cut(s) 413
BstDEI CTNAG 1 cut(s) 579
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 5 cut(s) 184, 537, 704, 938, 1030
BstFNI CGCG 4 cut(s) 356, 750, 985, 1001
BstHHI GCGC 1 cut(s) 750
BstKTI GATC 4 cut(s) 382, 421, 646, 696
BstMBI GATC 4 cut(s) 379, 418, 643, 693
BstMCI CGRYCG 1 cut(s) 696
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNSI RCATGY 2 cut(s) 415, 889
BstSCI CCNGG 2 cut(s) 208, 341
BstSFI CTRYAG 2 cut(s) 406, 591
BstSLI GKGCMC 1 cut(s) 995
BstUI CGCG 4 cut(s) 356, 750, 985, 1001
BstV1I GCAGC 2 cut(s) 837, 923
BstV2I GAAGAC 2 cut(s) 16, 294
BstX2I RGATCY 1 cut(s) 643
BstYI RGATCY 1 cut(s) 643
BsuI GTATCC 1 cut(s) 1015
BsuRI GGCC 3 cut(s) 156, 266, 964
BtgI CCRYGG 1 cut(s) 129
BtrI CACGTC 1 cut(s) 990
BtsCI GGATG 5 cut(s) 184, 537, 704, 938, 1030
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 2 cut(s) 72, 503
BveI ACCTGC 1 cut(s) 77
Cac8I GCNNGC 1 cut(s) 413
CaiI CAGNNNCTG 1 cut(s) 646
CfoI GCGC 1 cut(s) 750
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 962
CseI GACGC 4 cut(s) 345, 731, 990, 1007
Csp6I GTAC 4 cut(s) 17, 232, 601, 649
CviAII CATG 6 cut(s) 145, 329, 412, 673, 877, 886
CviQI GTAC 4 cut(s) 17, 232, 601, 649
DdeI CTNAG 1 cut(s) 579
DpnI GATC 4 cut(s) 381, 420, 645, 695
DpnII GATC 4 cut(s) 379, 418, 643, 693
Eco57I CTGAAG 1 cut(s) 1059
Eco88I CYCGRG 1 cut(s) 626
EcoO109I RGGNCCY 1 cut(s) 962
FaeI CATG 6 cut(s) 148, 332, 415, 676, 880, 889
FaqI GGGAC 3 cut(s) 203, 291, 538
FatI CATG 6 cut(s) 144, 328, 411, 672, 876, 885
FauI CCCGC 1 cut(s) 673
FblI GTMKAC 1 cut(s) 1062
Fnu4HI GCNGC 3 cut(s) 727, 851, 937
FokI GGATG 5 cut(s) 171, 524, 711, 945, 1017
Fsp4HI GCNGC 3 cut(s) 727, 851, 937
FspBI CTAG 3 cut(s) 20, 374, 840
GlaI GCGC 1 cut(s) 749
GluI GCNGC 3 cut(s) 727, 851, 937
HaeIII GGCC 3 cut(s) 156, 266, 964
HapII CCGG 4 cut(s) 141, 209, 225, 342
HgaI GACGC 4 cut(s) 345, 731, 990, 1007
HhaI GCGC 1 cut(s) 750
Hin1II CATG 6 cut(s) 148, 332, 415, 676, 880, 889
Hin6I GCGC 1 cut(s) 748
HinP1I GCGC 1 cut(s) 748
HincII GTYRAC 2 cut(s) 256, 396
HindII GTYRAC 2 cut(s) 256, 396
HinfI GANTC 2 cut(s) 125, 279
HpaI GTTAAC 1 cut(s) 396
HpaII CCGG 4 cut(s) 141, 209, 225, 342
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 5 cut(s) 136, 256, 396, 585, 1063
Hpy188I TCNGA 4 cut(s) 63, 384, 698, 783
Hpy188III TCNNGA 3 cut(s) 360, 541, 641
Hpy8I GTNNAC 5 cut(s) 136, 256, 396, 585, 1063
Hpy99I CGWCG 4 cut(s) 67, 361, 747, 1001
HpyAV CCTTC 4 cut(s) 222, 713, 818, 923
HpyCH4III ACNGT 7 cut(s) 16, 133, 507, 653, 779, 1027, 1081
HpyCH4IV ACGT 2 cut(s) 546, 989
HpyCH4V TGCA 9 cut(s) 72, 180, 391, 415, 503, 756, 823, 919, 1087
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 1 cut(s) 579
HpySE526I ACGT 2 cut(s) 546, 989
Hsp92II CATG 6 cut(s) 148, 332, 415, 676, 880, 889
HspAI GCGC 1 cut(s) 748
KspAI GTTAAC 1 cut(s) 396
Kzo9I GATC 4 cut(s) 379, 418, 643, 693
LmnI GCTCC 3 cut(s) 621, 659, 804
Lsp1109I GCAGC 2 cut(s) 837, 923
LweI GCATC 4 cut(s) 689, 738, 765, 923
MaeI CTAG 3 cut(s) 20, 374, 840
MaeII ACGT 2 cut(s) 546, 989
MaeIII GTNAC 1 cut(s) 10
MalI GATC 4 cut(s) 381, 420, 645, 695
MboI GATC 4 cut(s) 379, 418, 643, 693
MboII GAAGA 3 cut(s) 16, 299, 487
MflI RGATCY 1 cut(s) 643
MhlI GDGCHC 2 cut(s) 974, 995
MluCI AATT 1 cut(s) 1096
MluI ACGCGT 1 cut(s) 999
MlyI GAGTC 1 cut(s) 273
MmeI TCCRAC 2 cut(s) 86, 405
MseI TTAA 2 cut(s) 395, 456
MslI CAYNNNNRTG 1 cut(s) 143
MspI CCGG 4 cut(s) 141, 209, 225, 342
MspR9I CCNGG 2 cut(s) 210, 343
MvnI CGCG 4 cut(s) 356, 750, 985, 1001
MwoI GCNNNNNNNGC 1 cut(s) 113
NciI CCSGG 2 cut(s) 210, 343
NdeII GATC 4 cut(s) 379, 418, 643, 693
NlaIII CATG 6 cut(s) 148, 332, 415, 676, 880, 889
NlaIV GGNNCC 1 cut(s) 963
NmuCI GTSAC 1 cut(s) 10
NspI RCATGY 2 cut(s) 415, 889
PaeI GCATGC 1 cut(s) 415
PciI ACATGT 1 cut(s) 885
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PfeI GAWTC 1 cut(s) 125
PkrI GCNGC 3 cut(s) 728, 852, 938
Ple19I CGATCG 1 cut(s) 696
PleI GAGTC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 273
PscI ACATGT 1 cut(s) 885
PspN4I GGNNCC 1 cut(s) 963
PspPI GGNCC 1 cut(s) 962
PstNI CAGNNNCTG 1 cut(s) 646
PsuI RGATCY 1 cut(s) 643
PvuI CGATCG 1 cut(s) 696
RsaI GTAC 4 cut(s) 18, 233, 602, 650
RsaNI GTAC 4 cut(s) 17, 232, 601, 649
RseI CAYNNNNRTG 1 cut(s) 143
SaqAI TTAA 2 cut(s) 395, 456
SatI GCNGC 3 cut(s) 727, 851, 937
Sau3AI GATC 4 cut(s) 379, 418, 643, 693
Sau96I GGNCC 1 cut(s) 962
ScaI AGTACT 2 cut(s) 18, 602
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 2 cut(s) 210, 343
SduI GDGCHC 2 cut(s) 974, 995
SfaNI GCATC 4 cut(s) 689, 738, 765, 923
SfcI CTRYAG 2 cut(s) 406, 591
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 2 cut(s) 281, 814
SmoI CTYRAG 2 cut(s) 281, 814
SpeI ACTAGT 1 cut(s) 19
SphI GCATGC 1 cut(s) 415
Sse9I AATT 1 cut(s) 1096
SsiI CCGC 7 cut(s) 53, 667, 680, 707, 726, 939, 985
SspMI CTAG 3 cut(s) 20, 374, 840
StyD4I CCNGG 2 cut(s) 208, 341
TaaI ACNGT 7 cut(s) 16, 133, 507, 653, 779, 1027, 1081
TaiI ACGT 2 cut(s) 549, 992
TaqI TCGA 1 cut(s) 309
TasI AATT 1 cut(s) 1096
TatI WGTACW 3 cut(s) 16, 600, 648
TauI GCSGC 1 cut(s) 729
TfiI GAWTC 1 cut(s) 125
Tru1I TTAA 2 cut(s) 395, 456
Tru9I TTAA 2 cut(s) 395, 456
TscAI CASTG 2 cut(s) 79, 510
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 2 cut(s) 850, 936
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 4 cut(s) 161, 453, 661, 1019
TspRI CASTG 2 cut(s) 79, 510
XceI RCATGY 2 cut(s) 415, 889
XmiI GTMKAC 1 cut(s) 1062
XspI CTAG 3 cut(s) 20, 374, 840
ZrmI AGTACT 2 cut(s) 18, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.