MD07G1225100.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013832
Physical Location & Seq
Forward (+)
30167598 .. 30169944
2347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1225100.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGAGCAAAAAACCACCGTCCTCTTTGTACTCTTATTCTCTTTGGCCTCCTTTCTATCTCTACCAATCCCATCCACCGCATCTCCGCCTGTATCCGCTGTTTTTGCCTTCGGTGATTCAACCGTGGATTCGGGCAACAATAACCGCCTGCCATTCACCCTTTTCAGAAGTGACCACCCTCCATACGGCCGGGACTTGCCAAACCATGTCCCGTCCGGAAGGTTTACCAATGGAAAACTATCAACGGACTTCATGGTCTCCAGACTTGGCCTCAAGGACTTGTTACCTGCTTACCGCGATCTGAGACTGACCGACCGTGAATTGCTAACGGGTGTCAGTTTCGCATCAGGCGGTAGCGGGCTGGATGATCTGACTTTGGCCGTGTCAAAAGCCACGAGTTTGGGGAGGCAGTTGGAGGAATTTGATGAAGCGTTGGGGAGGATGAGGAGAACTGTTGGGGAGAAGAATAGTAGTGAGATTGTAAACAACGCTGTGTTTGTGATTAGTGTTGGTACTAATGACATGGTGTTTAACCTCTTCGATCTACCTGTTACTGCGAGGAAAATCGAGTTTTCACCTTCAGGATACCAGGATTTTCTTCTCCGCGGACTCGGGTCTTTCATTCAGAACCTGTACCAAAGGGGAGCGAGGAGATTCCAGGTGGCAGGTCTTCCACCAATCGGATGTCTGCCAATGCAGCTGACAATTAGCAGCATCTTTAACGGGCTTCGGCGTGTGTGCGTAGAACAGCAAAACACGGACTGTCAGGCTTACAACACCAAGCTCCTAGGCCTCATTCCTAGGTTGCAAGCCTCCCTTCCCGGATCCAGGCTCGCCTATTTCGACTCCTACATTCCCATCTTGGACATGTTCAACAACCCATCTAAATATGGGTTTGTGCAAACGCATAAAGGATGCTGTGGTACAGGGATGGTGGAGTTGGGCCCACTGTGCGGGGAGCTTTCGCTGACGTGTCCAGACCCTTCTAAATTTTTGTTCTGGGATTCCATGCATCCTTCCCAAGCAGCATACAAAGTTCTTGCTGACATATCTGCGAGGACGGTCTTACCGATCCTTACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

362

Amino Acids

39.54

Weight (kDa)

8.13

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 349 2e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40250
fragaria_vesca FvH4_7g24850 FvH4_7g24850
malus_domestica MD01G1156400.v1.1 MD07G1225100.v1.1
prunus_persica Prupe.2G253600_v2.0.a1
pyrus_communis pycom07g20380
rosa_chinensis RchiOBHm_Chr1g0370731
rosa_laevigata RLG00000027015
rosa_multiflora Rmu_co8466073.1_g000001 Rmu_sc0004550.1_g000013
rosa_rugosa Rorug01G0358700
rosa_samantha Rh1AG368400 Rh1BG331900 Rh1CG345800 Rh1DG363300
rosa_wichuraiana Rw1G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 254
Acc36I ACCTGC 2 cut(s) 295, 656
AccII CGCG 2 cut(s) 297, 606
AccIII TCCGGA 1 cut(s) 215
AclWI GGATC 3 cut(s) 819, 832, 1066
AcoI YGGCCR 2 cut(s) 187, 378
AcsI RAATTY 2 cut(s) 419, 989
AcuI CTGAAG 1 cut(s) 564
AfaI GTAC 4 cut(s) 30, 514, 635, 925
AfiI CCNNNNNNNGG 4 cut(s) 185, 680, 828, 953
AflIII ACRYGT 2 cut(s) 867, 971
AgsI TTSAA 2 cut(s) 120, 874
AjiI CACGTC 1 cut(s) 972
AjnI CCWGG 3 cut(s) 588, 657, 827
AluBI AGCT 3 cut(s) 700, 784, 961
AluI AGCT 3 cut(s) 700, 784, 961
Alw26I GTCTC 2 cut(s) 262, 298
AlwI GGATC 3 cut(s) 819, 832, 1066
AlwNI CAGNNNCTG 1 cut(s) 631
Ama87I CYCGRG 1 cut(s) 611
Aor13HI TCCGGA 1 cut(s) 215
AoxI GGCC 6 cut(s) 45, 187, 268, 378, 790, 943
ApaI GGGCCC 1 cut(s) 947
ApeKI GCWGC 3 cut(s) 697, 711, 1025
ApoI RAATTY 2 cut(s) 419, 989
AspA2I CCTAGG 2 cut(s) 787, 800
AspS9I GGNCC 2 cut(s) 943, 944
AsuC2I CCSGG 2 cut(s) 191, 822
AsuHPI GGTGA 3 cut(s) 125, 148, 567
AvaI CYCGRG 1 cut(s) 611
AvrII CCTAGG 2 cut(s) 787, 800
BaeGI GKGCMC 1 cut(s) 947
BamHI GGATCC 1 cut(s) 824
BanII GRGCYC 1 cut(s) 947
BauI CACGAG 1 cut(s) 394
BbsI GAAGAC 1 cut(s) 662
BbvI GCAGC 3 cut(s) 709, 723, 1037
BccI CCATC 4 cut(s) 79, 866, 889, 925
BceAI ACGGC 2 cut(s) 202, 365
BciT130I CCWGG 3 cut(s) 590, 659, 829
BciVI GTATCC 2 cut(s) 103, 578
BcnI CCSGG 2 cut(s) 191, 822
BcoDI GTCTC 2 cut(s) 262, 298
BfaI CTAG 2 cut(s) 788, 801
BfuAI ACCTGC 2 cut(s) 295, 656
BfuI GTATCC 2 cut(s) 103, 578
BisI GCNGC 3 cut(s) 698, 712, 1026
BlnI CCTAGG 2 cut(s) 787, 800
BlsI GCNGC 3 cut(s) 699, 713, 1027
Bme1390I CCNGG 5 cut(s) 191, 590, 659, 822, 829
BmeT110I CYCGRG 1 cut(s) 611
BmgBI CACGTC 1 cut(s) 972
BmgT120I GGNCC 2 cut(s) 943, 944
BmiI GGNNCC 2 cut(s) 826, 945
BmrFI CCNGG 5 cut(s) 191, 590, 659, 822, 829
BmsI GCATC 5 cut(s) 89, 353, 723, 905, 1021
BoxI GACNNNNGTC 1 cut(s) 613
BpiI GAAGAC 1 cut(s) 662
BpmI CTGGAG 1 cut(s) 244
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 2 cut(s) 191, 822
BsaI GGTCTC 1 cut(s) 262
BsaJI CCNNGG 4 cut(s) 123, 604, 787, 800
BsaWI WCCGGW 1 cut(s) 215
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 4 cut(s) 185, 680, 828, 953
BseAI TCCGGA 1 cut(s) 215
BseBI CCWGG 3 cut(s) 590, 659, 829
BseDI CCNNGG 4 cut(s) 123, 604, 787, 800
BseGI GGATG 7 cut(s) 71, 370, 447, 689, 920, 936, 1012
BseLI CCNNNNNNNGG 4 cut(s) 185, 680, 828, 953
BseMII CTCAG 1 cut(s) 293
BseRI GAGGAG 2 cut(s) 460, 664
BseSI GKGCMC 1 cut(s) 947
BseX3I CGGCCG 1 cut(s) 187
BseXI GCAGC 3 cut(s) 709, 723, 1037
Bsh1236I CGCG 2 cut(s) 297, 606
Bsh1285I CGRYCG 2 cut(s) 190, 316
BshFI GGCC 6 cut(s) 47, 189, 270, 380, 792, 945
BsiEI CGRYCG 2 cut(s) 190, 316
BsiHKCI CYCGRG 1 cut(s) 611
BsiSI CCGG 3 cut(s) 190, 216, 822
BslFI GGGAC 2 cut(s) 194, 206
BslI CCNNNNNNNGG 4 cut(s) 185, 680, 828, 953
BsmAI GTCTC 2 cut(s) 262, 298
BsmFI GGGAC 2 cut(s) 194, 206
BsnI GGCC 6 cut(s) 47, 189, 270, 380, 792, 945
Bso31I GGTCTC 1 cut(s) 262
BsoBI CYCGRG 1 cut(s) 611
Bsp120I GGGCCC 1 cut(s) 943
Bsp1286I GDGCHC 1 cut(s) 947
Bsp13I TCCGGA 1 cut(s) 215
Bsp143I GATC 5 cut(s) 298, 367, 541, 824, 1071
BspANI GGCC 6 cut(s) 47, 189, 270, 380, 792, 945
BspCNI CTCAG 1 cut(s) 294
BspEI TCCGGA 1 cut(s) 215
BspFNI CGCG 2 cut(s) 297, 606
BspLI GGNNCC 2 cut(s) 826, 945
BspMI ACCTGC 2 cut(s) 295, 656
BspPI GGATC 3 cut(s) 819, 832, 1066
BspTNI GGTCTC 1 cut(s) 262
BssECI CCNNGG 4 cut(s) 123, 604, 787, 800
BssMI GATC 5 cut(s) 298, 367, 541, 824, 1071
BssSI CACGAG 1 cut(s) 394
BssT1I CCWWGG 2 cut(s) 787, 800
Bst2BI CACGAG 1 cut(s) 394
Bst2UI CCWGG 3 cut(s) 590, 659, 829
Bst4CI ACNGT 7 cut(s) 19, 124, 317, 454, 764, 951, 1063
Bst6I CTCTTC 1 cut(s) 542
BstC8I GCNNGC 4 cut(s) 149, 359, 810, 834
BstDEI CTNAG 1 cut(s) 302
BstDSI CCRYGG 2 cut(s) 123, 604
BstF5I GGATG 7 cut(s) 71, 370, 447, 689, 920, 936, 1012
BstFNI CGCG 2 cut(s) 297, 606
BstKTI GATC 5 cut(s) 301, 370, 544, 827, 1074
BstMAI GTCTC 2 cut(s) 262, 298
BstMBI GATC 5 cut(s) 298, 367, 541, 824, 1071
BstMCI CGRYCG 2 cut(s) 190, 316
BstMWI GCNNNNNNNGC 3 cut(s) 104, 697, 951
BstNI CCWGG 3 cut(s) 590, 659, 829
BstNSI RCATGY 1 cut(s) 871
BstPAI GACNNNNGTC 1 cut(s) 613
BstSCI CCNGG 5 cut(s) 189, 588, 657, 820, 827
BstSLI GKGCMC 1 cut(s) 947
BstUI CGCG 2 cut(s) 297, 606
BstV1I GCAGC 3 cut(s) 709, 723, 1037
BstV2I GAAGAC 1 cut(s) 662
BstX2I RGATCY 1 cut(s) 824
BstXI CCANNNNNNTGG 1 cut(s) 400
BstYI RGATCY 1 cut(s) 824
BstZI CGGCCG 1 cut(s) 187
BsuI GTATCC 2 cut(s) 103, 578
BsuRI GGCC 6 cut(s) 47, 189, 270, 380, 792, 945
BtgI CCRYGG 2 cut(s) 123, 604
BtrI CACGTC 1 cut(s) 972
BtsCI GGATG 7 cut(s) 71, 370, 447, 689, 920, 936, 1012
BtsIMutI CAGTG 1 cut(s) 947
BveI ACCTGC 2 cut(s) 295, 656
Cac8I GCNNGC 4 cut(s) 149, 359, 810, 834
CaiI CAGNNNCTG 1 cut(s) 631
Cfr13I GGNCC 2 cut(s) 943, 944
Cfr42I CCGCGG 1 cut(s) 607
Csp6I GTAC 4 cut(s) 29, 513, 634, 924
CviAII CATG 5 cut(s) 206, 253, 523, 868, 1009
CviQI GTAC 4 cut(s) 29, 513, 634, 924
DdeI CTNAG 1 cut(s) 302
DpnI GATC 5 cut(s) 300, 369, 543, 826, 1073
DpnII GATC 5 cut(s) 298, 367, 541, 824, 1071
DrdI GACNNNNNNGTC 1 cut(s) 254
DseDI GACNNNNNNGTC 1 cut(s) 254
EaeI YGGCCR 2 cut(s) 187, 378
EagI CGGCCG 1 cut(s) 187
Eam1104I CTCTTC 1 cut(s) 542
EarI CTCTTC 1 cut(s) 542
EciI GGCGGA 1 cut(s) 75
EclXI CGGCCG 1 cut(s) 187
Eco130I CCWWGG 2 cut(s) 787, 800
Eco147I AGGCCT 1 cut(s) 792
Eco24I GRGCYC 1 cut(s) 947
Eco31I GGTCTC 1 cut(s) 262
Eco52I CGGCCG 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 564
Eco88I CYCGRG 1 cut(s) 611
EcoRII CCWGG 3 cut(s) 588, 657, 827
EcoT14I CCWWGG 2 cut(s) 787, 800
EcoT22I ATGCAT 1 cut(s) 1014
EcoT38I GRGCYC 1 cut(s) 947
ErhI CCWWGG 2 cut(s) 787, 800
FaeI CATG 5 cut(s) 209, 256, 526, 871, 1012
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FaqI GGGAC 2 cut(s) 194, 206
FatI CATG 5 cut(s) 205, 252, 522, 867, 1008
FauI CCCGC 2 cut(s) 350, 947
Fnu4HI GCNGC 3 cut(s) 698, 712, 1026
FokI GGATG 7 cut(s) 58, 377, 454, 696, 927, 943, 999
FriOI GRGCYC 1 cut(s) 947
Fsp4HI GCNGC 3 cut(s) 698, 712, 1026
FspBI CTAG 2 cut(s) 788, 801
GluI GCNGC 3 cut(s) 698, 712, 1026
GsuI CTGGAG 1 cut(s) 244
HaeIII GGCC 6 cut(s) 47, 189, 270, 380, 792, 945
HapII CCGG 3 cut(s) 190, 216, 822
Hin1II CATG 5 cut(s) 209, 256, 526, 871, 1012
HinfI GANTC 6 cut(s) 116, 128, 609, 654, 845, 1004
HpaII CCGG 3 cut(s) 190, 216, 822
HphI GGTGA 3 cut(s) 125, 148, 567
Hpy166II GTNNAC 2 cut(s) 225, 484
Hpy188I TCNGA 5 cut(s) 167, 303, 372, 627, 683
Hpy188III TCNNGA 4 cut(s) 216, 261, 582, 977
Hpy8I GTNNAC 2 cut(s) 225, 484
HpyAV CCTTC 6 cut(s) 118, 213, 588, 827, 993, 1026
HpyCH4III ACNGT 7 cut(s) 19, 124, 317, 454, 764, 951, 1063
HpyCH4IV ACGT 1 cut(s) 971
HpyCH4V TGCA 4 cut(s) 697, 808, 901, 1012
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 697, 951
HpyF3I CTNAG 1 cut(s) 302
HpySE526I ACGT 1 cut(s) 971
Hsp92II CATG 5 cut(s) 209, 256, 526, 871, 1012
Kpn2I TCCGGA 1 cut(s) 215
KspI CCGCGG 1 cut(s) 607
Kzo9I GATC 5 cut(s) 298, 367, 541, 824, 1071
LmnI GCTCC 4 cut(s) 4, 644, 789, 958
Lsp1109I GCAGC 3 cut(s) 709, 723, 1037
LweI GCATC 5 cut(s) 89, 353, 723, 905, 1021
MaeI CTAG 2 cut(s) 788, 801
MaeII ACGT 1 cut(s) 971
MaeIII GTNAC 3 cut(s) 170, 282, 550
MalI GATC 5 cut(s) 300, 369, 543, 826, 1073
MboI GATC 5 cut(s) 298, 367, 541, 824, 1071
MboII GAAGA 4 cut(s) 475, 529, 590, 662
MflI RGATCY 1 cut(s) 824
MhlI GDGCHC 1 cut(s) 947
MluCI AATT 4 cut(s) 320, 419, 705, 989
MlyI GAGTC 2 cut(s) 603, 839
MmeI TCCRAC 1 cut(s) 393
Mph1103I ATGCAT 1 cut(s) 1014
MroI TCCGGA 1 cut(s) 215
MseI TTAA 2 cut(s) 531, 720
MspA1I CMGCKG 3 cut(s) 98, 606, 700
MspI CCGG 3 cut(s) 190, 216, 822
MspR9I CCNGG 5 cut(s) 191, 590, 659, 822, 829
MvaI CCWGG 3 cut(s) 590, 659, 829
MvnI CGCG 2 cut(s) 297, 606
MwoI GCNNNNNNNGC 3 cut(s) 104, 697, 951
NciI CCSGG 2 cut(s) 191, 822
NdeII GATC 5 cut(s) 298, 367, 541, 824, 1071
NlaIII CATG 5 cut(s) 209, 256, 526, 871, 1012
NlaIV GGNNCC 2 cut(s) 826, 945
NmuCI GTSAC 1 cut(s) 170
NsiI ATGCAT 1 cut(s) 1014
NspI RCATGY 1 cut(s) 871
PceI AGGCCT 1 cut(s) 792
PciI ACATGT 1 cut(s) 867
PcsI WCGNNNNNNNCGW 2 cut(s) 840, 1067
PfeI GAWTC 4 cut(s) 116, 128, 654, 1004
PfoI TCCNGGA 1 cut(s) 820
PkrI GCNGC 3 cut(s) 699, 713, 1027
PleI GAGTC 2 cut(s) 603, 839
PpsI GAGTC 2 cut(s) 603, 839
PscI ACATGT 1 cut(s) 867
PshAI GACNNNNGTC 1 cut(s) 613
Psp6I CCWGG 3 cut(s) 588, 657, 827
PspGI CCWGG 3 cut(s) 588, 657, 827
PspN4I GGNNCC 2 cut(s) 826, 945
PspOMI GGGCCC 1 cut(s) 943
PspPI GGNCC 2 cut(s) 943, 944
PstNI CAGNNNCTG 1 cut(s) 631
PsuI RGATCY 1 cut(s) 824
PvuII CAGCTG 1 cut(s) 700
RsaI GTAC 4 cut(s) 30, 514, 635, 925
RsaNI GTAC 4 cut(s) 29, 513, 634, 924
SacII CCGCGG 1 cut(s) 607
SaqAI TTAA 2 cut(s) 531, 720
SatI GCNGC 3 cut(s) 698, 712, 1026
Sau3AI GATC 5 cut(s) 298, 367, 541, 824, 1071
Sau96I GGNCC 2 cut(s) 943, 944
SchI GAGTC 2 cut(s) 603, 839
ScrFI CCNGG 5 cut(s) 191, 590, 659, 822, 829
SduI GDGCHC 1 cut(s) 947
SfaNI GCATC 5 cut(s) 89, 353, 723, 905, 1021
Sfr303I CCGCGG 1 cut(s) 607
SgrBI CCGCGG 1 cut(s) 607
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 4 cut(s) 320, 419, 705, 989
SseBI AGGCCT 1 cut(s) 792
SspMI CTAG 2 cut(s) 788, 801
StuI AGGCCT 1 cut(s) 792
StyD4I CCNGG 5 cut(s) 189, 588, 657, 820, 827
StyI CCWWGG 2 cut(s) 787, 800
TaaI ACNGT 7 cut(s) 19, 124, 317, 454, 764, 951, 1063
TaiI ACGT 1 cut(s) 974
TaqI TCGA 3 cut(s) 540, 567, 843
TaqII GACCGA 1 cut(s) 326
TasI AATT 4 cut(s) 320, 419, 705, 989
TatI WGTACW 1 cut(s) 28
TfiI GAWTC 4 cut(s) 116, 128, 654, 1004
Tru1I TTAA 2 cut(s) 531, 720
Tru9I TTAA 2 cut(s) 531, 720
TscAI CASTG 1 cut(s) 954
TseFI GTSAC 1 cut(s) 170
TseI GCWGC 3 cut(s) 697, 711, 1025
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 3 cut(s) 241, 441, 610
TspGWI ACGGA 2 cut(s) 260, 773
TspRI CASTG 1 cut(s) 954
XapI RAATTY 2 cut(s) 419, 989
XceI RCATGY 1 cut(s) 871
XmaJI CCTAGG 2 cut(s) 787, 800
XspI CTAG 2 cut(s) 788, 801
Zsp2I ATGCAT 1 cut(s) 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.