RLG00000027015

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013832
Physical Location & Seq
Reverse (-)
7679726 .. 7681656
1931 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027015

Sequence Viewer

Length: 1077 bp
ATGTTCACACTATTCTTCTCTTTGGCCACCTTGATAATCCTACCGCCATCTCCGATCAGTGCCTCCTCTCCATCTGTTTCTGCTCTCTTCGCTTTTGGAGATTCCACGGTGGATTCCGGCATGAACAACGACCTCATGCCTTTCGTTCGGTGCGACCATCCCCCTTACGGCAGAGACCTGCCGGGACACGTCCCCACCGGAAGGTACTCCAATGGCAAGATTGCAACTGACTTTATGGCCTCAAACTTGGGACTCAAGGACCTCTTGCCCGCTTACCTCGACCCGAAATTGACCGACTATGATTTGCTCACCGGGGTCAGTTTTGCGTCGGGAACTTGCGGCCTAGATGATCTGACCTTGGAGTTGAGCCACGTCTATAGTATGGGGGATCAGTTGGAGTATTTTGATGAAGCCGTAGTGAGGATGAAGATAAGTGTTGGGGAAAAGAAGGGTAGAGAGATTGTGGAAAATGCACTGTTTGTGGTCAGTGTTGGGACGAATGACTTGATGCTGAACGTGTATGAGTTTCCTCTAACCGAGAGGAAAACTCAGTTTACGTCTACAGACCAGTACTCTGATTTTCTGCTCCAGAGACTTGGGTCTTTTATTCAGGCTCTGTACAGTAGGGGAGCACGGCGGTTCATGGTGGCGGGTCTTCCACCGATCGGTTGCCTTCCGCTTCAACGGACACTGAGCGGCATCATCAATCTGCGCCCTCAGCGCCTGTGCGTCACTCAGCAAATCATAGACTGTCAGGCCTACAACTCCAAGCTCCAGGCCCTTCTCTCAAGTTTGCACACCACATTACCACTCCCTAGTTCAAGGCTAGTCTATTTTGACATCTACAACCCCATCATGGACATGTACAACAACCCAGCTAAATATGGGTTTGTGCATATACATGAAGGATGCTGTGGAACTGGGATGCTTGAGTTGGGGCCTCTGTGCACAGAGCTTTCGCGGACGTGCCCCGACGCGTCGAAGTATCTTTTCTGGGATGCAGTTCATCTTACTGAAGCAGCATTTTTCGTCATTGCTGACTATGCTCATAAGACTGTCTTGCAGTACCTTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

359

Amino Acids

39.69

Weight (kDa)

5.34

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 346 1.5e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40250
fragaria_vesca FvH4_7g24850 FvH4_7g24850
malus_domestica MD01G1156400.v1.1 MD07G1225100.v1.1
prunus_persica Prupe.2G253600_v2.0.a1
pyrus_communis pycom07g20380
rosa_chinensis RchiOBHm_Chr1g0370731
rosa_laevigata RLG00000027015
rosa_multiflora Rmu_co8466073.1_g000001 Rmu_sc0004550.1_g000013
rosa_rugosa Rorug01G0358700
rosa_samantha Rh1AG368400 Rh1BG331900 Rh1CG345800 Rh1DG363300
rosa_wichuraiana Rw1G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 186
AccBSI CCGCTC 1 cut(s) 696
AccI GTMKAC 1 cut(s) 560
AccII CGCG 2 cut(s) 961, 977
AciI CCGC 8 cut(s) 44, 270, 339, 637, 650, 677, 696, 961
AclWI GGATC 1 cut(s) 396
AcoI YGGCCR 1 cut(s) 24
AcuI CTGAAG 1 cut(s) 1035
AfaI GTAC 5 cut(s) 206, 572, 620, 866, 1067
AfiI CCNNNNNNNGG 5 cut(s) 167, 201, 420, 665, 856
AflIII ACRYGT 4 cut(s) 187, 516, 861, 975
AgsI TTSAA 2 cut(s) 683, 822
AjiI CACGTC 3 cut(s) 190, 373, 966
AjnI CCWGG 1 cut(s) 774
AluBI AGCT 3 cut(s) 772, 878, 955
AluI AGCT 3 cut(s) 772, 878, 955
Alw21I GWGCWC 2 cut(s) 634, 950
Alw26I GTCTC 2 cut(s) 168, 586
Alw44I GTGCAC 1 cut(s) 946
AlwI GGATC 1 cut(s) 396
AlwNI CAGNNNCTG 2 cut(s) 616, 724
AoxI GGCC 6 cut(s) 24, 237, 340, 756, 777, 938
ApaLI GTGCAC 1 cut(s) 946
ApeKI GCWGC 1 cut(s) 1019
AspLEI GCGC 2 cut(s) 714, 723
AspS9I GGNCC 3 cut(s) 259, 778, 938
AsuC2I CCSGG 2 cut(s) 183, 313
AsuHPI GGTGA 1 cut(s) 301
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 2 cut(s) 950, 971
BalI TGGCCA 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 647
Bbv12I GWGCWC 2 cut(s) 634, 950
BbvCI CCTCAGC 1 cut(s) 717
BbvI GCAGC 1 cut(s) 1031
BccI CCATC 4 cut(s) 55, 79, 165, 860
BceAI ACGGC 3 cut(s) 184, 398, 650
BciT130I CCWGG 1 cut(s) 776
BcnI CCSGG 2 cut(s) 183, 313
BcoDI GTCTC 2 cut(s) 168, 586
BfaI CTAG 3 cut(s) 344, 816, 827
BfmI CTRYAG 2 cut(s) 376, 561
BfoI RGCGCY 1 cut(s) 724
BfuAI ACCTGC 1 cut(s) 186
BisI GCNGC 3 cut(s) 340, 697, 1020
BlsI GCNGC 3 cut(s) 341, 698, 1021
BmcAI AGTACT 1 cut(s) 572
Bme1390I CCNGG 3 cut(s) 183, 313, 776
Bme18I GGWCC 1 cut(s) 259
BmgBI CACGTC 3 cut(s) 190, 373, 966
BmgT120I GGNCC 3 cut(s) 259, 778, 938
BmiI GGNNCC 1 cut(s) 939
BmrFI CCNGG 3 cut(s) 183, 313, 776
BmrI ACTGGG 1 cut(s) 930
BmsI GCATC 5 cut(s) 498, 708, 899, 915, 988
BmuI ACTGGG 1 cut(s) 930
BoxI GACNNNNGTC 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 647
BplI GAGNNNNNCTC 2 cut(s) 532, 564
BpmI CTGGAG 2 cut(s) 572, 758
Bpu10I CCTNAGC 1 cut(s) 717
BpuEI CTTGAG 3 cut(s) 239, 772, 950
BpuMI CCSGG 2 cut(s) 183, 313
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 3 cut(s) 105, 312, 357
BsaWI WCCGGW 1 cut(s) 197
Bsc4I CCNNNNNNNGG 5 cut(s) 167, 201, 420, 665, 856
Bse1I ACTGG 2 cut(s) 568, 925
Bse3DI GCAATG 1 cut(s) 1032
BseBI CCWGG 1 cut(s) 776
BseDI CCNNGG 3 cut(s) 105, 312, 357
BseGI GGATG 5 cut(s) 157, 429, 914, 930, 1003
BseLI CCNNNNNNNGG 5 cut(s) 167, 201, 420, 665, 856
BseMI GCAATG 1 cut(s) 1032
BseMII CTCAG 4 cut(s) 563, 683, 731, 749
BseNI ACTGG 2 cut(s) 568, 925
BseRI GAGGAG 1 cut(s) 55
BseSI GKGCMC 2 cut(s) 950, 971
BseXI GCAGC 1 cut(s) 1031
BseYI CCCAGC 1 cut(s) 874
Bsh1236I CGCG 2 cut(s) 961, 977
Bsh1285I CGRYCG 1 cut(s) 666
BshFI GGCC 6 cut(s) 26, 239, 342, 758, 779, 940
BsiEI CGRYCG 1 cut(s) 666
BsiHKAI GWGCWC 2 cut(s) 634, 950
BsiSI CCGG 4 cut(s) 117, 182, 198, 312
BslFI GGGAC 4 cut(s) 176, 198, 264, 508
BslI CCNNNNNNNGG 5 cut(s) 167, 201, 420, 665, 856
BsmAI GTCTC 2 cut(s) 168, 586
BsmFI GGGAC 4 cut(s) 176, 198, 264, 508
BsnI GGCC 6 cut(s) 26, 239, 342, 758, 779, 940
Bso31I GGTCTC 1 cut(s) 168
Bsp1286I GDGCHC 3 cut(s) 634, 950, 971
Bsp1407I TGTACA 2 cut(s) 618, 864
Bsp143I GATC 4 cut(s) 54, 349, 388, 663
BspACI CCGC 8 cut(s) 44, 270, 339, 637, 650, 677, 696, 961
BspANI GGCC 6 cut(s) 26, 239, 342, 758, 779, 940
BspCNI CTCAG 4 cut(s) 562, 684, 730, 748
BspFNI CGCG 2 cut(s) 961, 977
BspLI GGNNCC 1 cut(s) 939
BspMI ACCTGC 1 cut(s) 186
BspPI GGATC 1 cut(s) 396
BspTNI GGTCTC 1 cut(s) 168
BsrBI CCGCTC 1 cut(s) 696
BsrDI GCAATG 1 cut(s) 1032
BsrGI TGTACA 2 cut(s) 618, 864
BsrI ACTGG 2 cut(s) 568, 925
BssECI CCNNGG 3 cut(s) 105, 312, 357
BssMI GATC 4 cut(s) 54, 349, 388, 663
BssT1I CCWWGG 1 cut(s) 357
Bst2UI CCWGG 1 cut(s) 776
Bst4CI ACNGT 5 cut(s) 109, 477, 623, 752, 1057
Bst6I CTCTTC 1 cut(s) 92
BstAUI TGTACA 2 cut(s) 618, 864
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 5 cut(s) 549, 692, 717, 735, 1070
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 5 cut(s) 157, 429, 914, 930, 1003
BstFNI CGCG 2 cut(s) 961, 977
BstH2I RGCGCY 1 cut(s) 724
BstHHI GCGC 2 cut(s) 714, 723
BstKTI GATC 4 cut(s) 57, 352, 391, 666
BstMAI GTCTC 2 cut(s) 168, 586
BstMBI GATC 4 cut(s) 54, 349, 388, 663
BstMCI CGRYCG 1 cut(s) 666
BstMWI GCNNNNNNNGC 4 cut(s) 89, 718, 720, 1043
BstNI CCWGG 1 cut(s) 776
BstNSI RCATGY 1 cut(s) 865
BstPAI GACNNNNGTC 1 cut(s) 598
BstSCI CCNGG 3 cut(s) 181, 311, 774
BstSFI CTRYAG 2 cut(s) 376, 561
BstSLI GKGCMC 2 cut(s) 950, 971
BstUI CGCG 2 cut(s) 961, 977
BstV1I GCAGC 1 cut(s) 1031
BstV2I GAAGAC 1 cut(s) 647
BstXI CCANNNNNNTGG 1 cut(s) 596
BsuRI GGCC 6 cut(s) 26, 239, 342, 758, 779, 940
BtgI CCRYGG 1 cut(s) 105
BtrI CACGTC 3 cut(s) 190, 373, 966
BtsCI GGATG 5 cut(s) 157, 429, 914, 930, 1003
BtsIMutI CAGTG 4 cut(s) 64, 473, 493, 689
BveI ACCTGC 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 270
CaiI CAGNNNCTG 2 cut(s) 616, 724
CfoI GCGC 2 cut(s) 714, 723
Cfr13I GGNCC 3 cut(s) 259, 778, 938
CseI GACGC 4 cut(s) 315, 718, 966, 983
Csp6I GTAC 5 cut(s) 205, 571, 619, 865, 1066
CviAII CATG 6 cut(s) 121, 136, 643, 856, 862, 902
CviQI GTAC 5 cut(s) 205, 571, 619, 865, 1066
DdeI CTNAG 5 cut(s) 549, 692, 717, 735, 1070
DpnI GATC 4 cut(s) 56, 351, 390, 665
DpnII GATC 4 cut(s) 54, 349, 388, 663
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 1 cut(s) 758
Eco31I GGTCTC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 1035
EcoO109I RGGNCCY 3 cut(s) 259, 778, 938
EcoRII CCWGG 1 cut(s) 774
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 6 cut(s) 124, 139, 646, 859, 865, 905
FalI AAGNNNNNCTT 4 cut(s) 248, 280, 1043, 1075
FaqI GGGAC 4 cut(s) 176, 198, 264, 508
FatI CATG 6 cut(s) 120, 135, 642, 855, 861, 901
FauI CCCGC 2 cut(s) 277, 643
FblI GTMKAC 1 cut(s) 560
Fnu4HI GCNGC 3 cut(s) 340, 697, 1020
FokI GGATG 5 cut(s) 144, 436, 921, 937, 1010
Fsp4HI GCNGC 3 cut(s) 340, 697, 1020
FspBI CTAG 3 cut(s) 344, 816, 827
GlaI GCGC 2 cut(s) 713, 722
GluI GCNGC 3 cut(s) 340, 697, 1020
GsaI CCCAGC 1 cut(s) 878
GsuI CTGGAG 2 cut(s) 572, 758
HaeII RGCGCY 1 cut(s) 724
HaeIII GGCC 6 cut(s) 26, 239, 342, 758, 779, 940
HapII CCGG 4 cut(s) 117, 182, 198, 312
HgaI GACGC 4 cut(s) 315, 718, 966, 983
HhaI GCGC 2 cut(s) 714, 723
Hin1II CATG 6 cut(s) 124, 139, 646, 859, 865, 905
Hin6I GCGC 2 cut(s) 712, 721
HinP1I GCGC 2 cut(s) 712, 721
HinfI GANTC 3 cut(s) 101, 113, 252
HpaII CCGG 4 cut(s) 117, 182, 198, 312
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 4 cut(s) 6, 555, 561, 948
Hpy188I TCNGA 3 cut(s) 54, 354, 577
Hpy188III TCNNGA 2 cut(s) 330, 589
Hpy8I GTNNAC 4 cut(s) 6, 555, 561, 948
Hpy99I CGWCG 3 cut(s) 331, 977, 982
HpyAV CCTTC 5 cut(s) 195, 442, 683, 791, 899
HpyCH4III ACNGT 5 cut(s) 109, 477, 623, 752, 1057
HpyCH4IV ACGT 5 cut(s) 189, 372, 516, 557, 965
HpyCH4V TGCA 7 cut(s) 224, 473, 796, 895, 948, 1001, 1063
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 718, 720, 1043
HpyF3I CTNAG 5 cut(s) 549, 692, 717, 735, 1070
HpySE526I ACGT 5 cut(s) 189, 372, 516, 557, 965
Hsp92II CATG 6 cut(s) 124, 139, 646, 859, 865, 905
HspAI GCGC 2 cut(s) 712, 721
Kzo9I GATC 4 cut(s) 54, 349, 388, 663
LmnI GCTCC 3 cut(s) 591, 629, 777
Lsp1109I GCAGC 1 cut(s) 1031
LweI GCATC 5 cut(s) 498, 708, 899, 915, 988
MaeI CTAG 3 cut(s) 344, 816, 827
MaeII ACGT 5 cut(s) 189, 372, 516, 557, 965
MaeIII GTNAC 1 cut(s) 730
MalI GATC 4 cut(s) 56, 351, 390, 665
MbiI CCGCTC 1 cut(s) 696
MboI GATC 4 cut(s) 54, 349, 388, 663
MboII GAAGA 4 cut(s) 7, 79, 439, 647
MhlI GDGCHC 3 cut(s) 634, 950, 971
MlsI TGGCCA 1 cut(s) 26
MluCI AATT 1 cut(s) 287
MluI ACGCGT 1 cut(s) 975
MluNI TGGCCA 1 cut(s) 26
MlyI GAGTC 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 375
Mox20I TGGCCA 1 cut(s) 26
MscI TGGCCA 1 cut(s) 26
MslI CAYNNNNRTG 2 cut(s) 860, 900
Msp20I TGGCCA 1 cut(s) 26
MspI CCGG 4 cut(s) 117, 182, 198, 312
MspR9I CCNGG 3 cut(s) 183, 313, 776
MvaI CCWGG 1 cut(s) 776
MvnI CGCG 2 cut(s) 961, 977
MwoI GCNNNNNNNGC 4 cut(s) 89, 718, 720, 1043
NciI CCSGG 2 cut(s) 183, 313
NdeII GATC 4 cut(s) 54, 349, 388, 663
NlaIII CATG 6 cut(s) 124, 139, 646, 859, 865, 905
NlaIV GGNNCC 1 cut(s) 939
NmuCI GTSAC 1 cut(s) 730
NspI RCATGY 1 cut(s) 865
PceI AGGCCT 1 cut(s) 758
PciI ACATGT 1 cut(s) 861
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PfeI GAWTC 2 cut(s) 101, 113
PkrI GCNGC 3 cut(s) 341, 698, 1021
Ple19I CGATCG 1 cut(s) 666
PleI GAGTC 1 cut(s) 246
PpsI GAGTC 1 cut(s) 246
PpuMI RGGWCCY 1 cut(s) 259
PscI ACATGT 1 cut(s) 861
PshAI GACNNNNGTC 1 cut(s) 598
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 774
PspFI CCCAGC 1 cut(s) 874
PspGI CCWGG 1 cut(s) 774
PspN4I GGNNCC 1 cut(s) 939
PspPI GGNCC 3 cut(s) 259, 778, 938
PspPPI RGGWCCY 1 cut(s) 259
PstNI CAGNNNCTG 2 cut(s) 616, 724
PvuI CGATCG 1 cut(s) 666
RsaI GTAC 5 cut(s) 206, 572, 620, 866, 1067
RsaNI GTAC 5 cut(s) 205, 571, 619, 865, 1066
RseI CAYNNNNRTG 2 cut(s) 860, 900
SatI GCNGC 3 cut(s) 340, 697, 1020
Sau3AI GATC 4 cut(s) 54, 349, 388, 663
Sau96I GGNCC 3 cut(s) 259, 778, 938
ScaI AGTACT 1 cut(s) 572
SchI GAGTC 1 cut(s) 246
ScrFI CCNGG 3 cut(s) 183, 313, 776
SduI GDGCHC 3 cut(s) 634, 950, 971
SfaNI GCATC 5 cut(s) 498, 708, 899, 915, 988
SfcI CTRYAG 2 cut(s) 376, 561
SinI GGWCC 1 cut(s) 259
SmiMI CAYNNNNRTG 2 cut(s) 860, 900
SmlI CTYRAG 3 cut(s) 254, 787, 929
SmoI CTYRAG 3 cut(s) 254, 787, 929
Sse9I AATT 1 cut(s) 287
SseBI AGGCCT 1 cut(s) 758
SsiI CCGC 8 cut(s) 44, 270, 339, 637, 650, 677, 696, 961
SspMI CTAG 3 cut(s) 344, 816, 827
StuI AGGCCT 1 cut(s) 758
StyD4I CCNGG 3 cut(s) 181, 311, 774
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 5 cut(s) 109, 477, 623, 752, 1057
TaiI ACGT 5 cut(s) 192, 375, 519, 560, 968
TaqI TCGA 2 cut(s) 279, 980
TaqII GACCGA 1 cut(s) 308
TasI AATT 1 cut(s) 287
TatI WGTACW 3 cut(s) 570, 618, 864
TauI GCSGC 2 cut(s) 342, 699
TfiI GAWTC 2 cut(s) 101, 113
TscAI CASTG 4 cut(s) 64, 480, 493, 696
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 1 cut(s) 1019
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 6 cut(s) 137, 423, 440, 631, 918, 995
TspGWI ACGGA 1 cut(s) 700
TspRI CASTG 4 cut(s) 64, 480, 493, 696
VneI GTGCAC 1 cut(s) 946
VpaK11BI GGWCC 1 cut(s) 259
XceI RCATGY 1 cut(s) 865
XmiI GTMKAC 1 cut(s) 560
XspI CTAG 3 cut(s) 344, 816, 827
ZrmI AGTACT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.