Rmu_co8466073.1_g000001

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8466073.1
OG0013832
Physical Location & Seq
Reverse (-)
443 .. 1020
578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8466073.1_g000001.1.cds

Sequence Viewer

Length: 471 bp
atgcagggtctgtacagtaggggagcacggcggttcatggtggcgggtcttccaccgatcggatgccttccgcttcaacggacactgagcagcatcatcaatctgcgccctcagcgcctgtgcgtcacgcagcaaaacatagactgtcagacctacaactccaagctccaggcccttctctcaagtttgcaaaccacactgccactccctagttcaaggctagtctattttgacatctacaaccccatcatggacatgtacaacaacccagataaatatggatttgtgcatatacatgaaggatgctgtggaactgggatgtttgagttggggcctctgtgcacagagttttcgcggacgtgccccgacgcgtcgaagtatcttttctgggatgcagttcatcctactgaagcagcatttttcgtcattgctgactatgctcataagactgtcttgcagtaccttagttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

156

Amino Acids

17.64

Weight (kDa)

7.58

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40250
fragaria_vesca FvH4_7g24850 FvH4_7g24850
malus_domestica MD01G1156400.v1.1 MD07G1225100.v1.1
prunus_persica Prupe.2G253600_v2.0.a1
pyrus_communis pycom07g20380
rosa_chinensis RchiOBHm_Chr1g0370731
rosa_laevigata RLG00000027015
rosa_multiflora Rmu_co8466073.1_g000001 Rmu_sc0004550.1_g000013
rosa_rugosa Rorug01G0358700
rosa_samantha Rh1AG368400 Rh1BG331900 Rh1CG345800 Rh1DG363300
rosa_wichuraiana Rw1G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 355, 371
AciI CCGC 4 cut(s) 31, 44, 71, 355
AcuI CTGAAG 1 cut(s) 429
AfaI GTAC 3 cut(s) 14, 260, 461
AfiI CCNNNNNNNGG 2 cut(s) 59, 250
AflIII ACRYGT 2 cut(s) 255, 369
AgsI TTSAA 2 cut(s) 77, 216
AjiI CACGTC 1 cut(s) 360
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 1 cut(s) 166
AluI AGCT 1 cut(s) 166
Alw21I GWGCWC 2 cut(s) 28, 344
Alw44I GTGCAC 1 cut(s) 340
AlwNI CAGNNNCTG 2 cut(s) 10, 118
AoxI GGCC 2 cut(s) 171, 332
ApaLI GTGCAC 1 cut(s) 340
ApeKI GCWGC 3 cut(s) 90, 130, 413
AspLEI GCGC 2 cut(s) 108, 117
AspS9I GGNCC 2 cut(s) 172, 332
BaeGI GKGCMC 2 cut(s) 344, 365
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 2 cut(s) 28, 344
BbvCI CCTCAGC 1 cut(s) 111
BbvI GCAGC 3 cut(s) 102, 142, 425
BccI CCATC 1 cut(s) 254
BceAI ACGGC 1 cut(s) 44
BciT130I CCWGG 1 cut(s) 170
BfaI CTAG 2 cut(s) 210, 221
BfoI RGCGCY 1 cut(s) 118
BisI GCNGC 3 cut(s) 91, 131, 414
BlsI GCNGC 3 cut(s) 92, 132, 415
Bme1390I CCNGG 1 cut(s) 170
BmgBI CACGTC 1 cut(s) 360
BmgT120I GGNCC 2 cut(s) 172, 332
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 1 cut(s) 170
BmrI ACTGGG 1 cut(s) 324
BmsI GCATC 4 cut(s) 53, 102, 293, 382
BmuI ACTGGG 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 41
BpmI CTGGAG 1 cut(s) 152
Bpu10I CCTNAGC 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 166
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 250
Bse1I ACTGG 1 cut(s) 319
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 1 cut(s) 170
BseGI GGATG 5 cut(s) 68, 308, 324, 397, 400
BseLI CCNNNNNNNGG 2 cut(s) 59, 250
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 2 cut(s) 77, 125
BseNI ACTGG 1 cut(s) 319
BseSI GKGCMC 2 cut(s) 344, 365
BseXI GCAGC 3 cut(s) 102, 142, 425
Bsh1236I CGCG 2 cut(s) 355, 371
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 173, 334
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 2 cut(s) 28, 344
BslI CCNNNNNNNGG 2 cut(s) 59, 250
BsnI GGCC 2 cut(s) 173, 334
Bsp1286I GDGCHC 3 cut(s) 28, 344, 365
Bsp1407I TGTACA 2 cut(s) 12, 258
Bsp143I GATC 1 cut(s) 57
BspACI CCGC 4 cut(s) 31, 44, 71, 355
BspANI GGCC 2 cut(s) 173, 334
BspCNI CTCAG 2 cut(s) 78, 124
BspFNI CGCG 2 cut(s) 355, 371
BspLI GGNNCC 1 cut(s) 333
BsrDI GCAATG 1 cut(s) 426
BsrGI TGTACA 2 cut(s) 12, 258
BsrI ACTGG 1 cut(s) 319
BssMI GATC 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 3 cut(s) 17, 146, 451
BstAUI TGTACA 2 cut(s) 12, 258
BstDEI CTNAG 3 cut(s) 86, 111, 464
BstF5I GGATG 5 cut(s) 68, 308, 324, 397, 400
BstFNI CGCG 2 cut(s) 355, 371
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 2 cut(s) 108, 117
BstKTI GATC 1 cut(s) 60
BstMBI GATC 1 cut(s) 57
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 3 cut(s) 112, 114, 437
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 168
BstSLI GKGCMC 2 cut(s) 344, 365
BstUI CGCG 2 cut(s) 355, 371
BstV1I GCAGC 3 cut(s) 102, 142, 425
BstV2I GAAGAC 1 cut(s) 41
BsuRI GGCC 2 cut(s) 173, 334
BtrI CACGTC 1 cut(s) 360
BtsCI GGATG 5 cut(s) 68, 308, 324, 397, 400
BtsI GCAGTG 1 cut(s) 197
BtsIMutI CAGTG 2 cut(s) 83, 197
CaiI CAGNNNCTG 2 cut(s) 10, 118
CfoI GCGC 2 cut(s) 108, 117
Cfr13I GGNCC 2 cut(s) 172, 332
CseI GACGC 3 cut(s) 112, 360, 377
Csp6I GTAC 3 cut(s) 13, 259, 460
CviAII CATG 4 cut(s) 37, 250, 256, 296
CviJI RGCY 4 cut(s) 166, 173, 220, 334
CviKI_1 RGCY 4 cut(s) 166, 173, 220, 334
CviQI GTAC 3 cut(s) 13, 259, 460
DdeI CTNAG 3 cut(s) 86, 111, 464
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
Eco57I CTGAAG 1 cut(s) 429
EcoO109I RGGNCCY 2 cut(s) 172, 332
EcoRII CCWGG 1 cut(s) 168
FaeI CATG 4 cut(s) 40, 253, 259, 299
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FatI CATG 4 cut(s) 36, 249, 255, 295
FauI CCCGC 1 cut(s) 37
Fnu4HI GCNGC 3 cut(s) 91, 131, 414
FokI GGATG 5 cut(s) 75, 315, 331, 387, 404
Fsp4HI GCNGC 3 cut(s) 91, 131, 414
FspBI CTAG 2 cut(s) 210, 221
GlaI GCGC 2 cut(s) 107, 116
GluI GCNGC 3 cut(s) 91, 131, 414
GsuI CTGGAG 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 2 cut(s) 173, 334
HgaI GACGC 3 cut(s) 112, 360, 377
HhaI GCGC 2 cut(s) 108, 117
Hin1II CATG 4 cut(s) 40, 253, 259, 299
Hin6I GCGC 2 cut(s) 106, 115
HinP1I GCGC 2 cut(s) 106, 115
Hpy166II GTNNAC 1 cut(s) 342
Hpy188I TCNGA 2 cut(s) 62, 150
Hpy8I GTNNAC 1 cut(s) 342
Hpy99I CGWCG 2 cut(s) 371, 376
HpyAV CCTTC 3 cut(s) 77, 185, 293
HpyCH4III ACNGT 3 cut(s) 17, 146, 451
HpyCH4IV ACGT 1 cut(s) 359
HpyCH4V TGCA 6 cut(s) 4, 190, 289, 342, 395, 457
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 114, 437
HpyF3I CTNAG 3 cut(s) 86, 111, 464
HpySE526I ACGT 1 cut(s) 359
Hsp92II CATG 4 cut(s) 40, 253, 259, 299
HspAI GCGC 2 cut(s) 106, 115
Kzo9I GATC 1 cut(s) 57
LmnI GCTCC 2 cut(s) 23, 171
LpnPI CCDG 6 cut(s) 131, 155, 182, 282, 300, 373
Lsp1109I GCAGC 3 cut(s) 102, 142, 425
LweI GCATC 4 cut(s) 53, 102, 293, 382
MaeI CTAG 2 cut(s) 210, 221
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 1 cut(s) 124
MalI GATC 1 cut(s) 59
MboI GATC 1 cut(s) 57
MboII GAAGA 1 cut(s) 41
MhlI GDGCHC 3 cut(s) 28, 344, 365
MluI ACGCGT 1 cut(s) 369
MnlI CCTC 2 cut(s) 120, 345
MslI CAYNNNNRTG 2 cut(s) 254, 294
MspR9I CCNGG 1 cut(s) 170
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 355, 371
MwoI GCNNNNNNNGC 3 cut(s) 112, 114, 437
NdeII GATC 1 cut(s) 57
NlaIII CATG 4 cut(s) 40, 253, 259, 299
NlaIV GGNNCC 1 cut(s) 333
NmuCI GTSAC 1 cut(s) 124
NspI RCATGY 1 cut(s) 259
PciI ACATGT 1 cut(s) 255
PkrI GCNGC 3 cut(s) 92, 132, 415
Ple19I CGATCG 1 cut(s) 60
PscI ACATGT 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 333
PspPI GGNCC 2 cut(s) 172, 332
PstNI CAGNNNCTG 2 cut(s) 10, 118
PvuI CGATCG 1 cut(s) 60
RsaI GTAC 3 cut(s) 14, 260, 461
RsaNI GTAC 3 cut(s) 13, 259, 460
RseI CAYNNNNRTG 2 cut(s) 254, 294
SatI GCNGC 3 cut(s) 91, 131, 414
Sau3AI GATC 1 cut(s) 57
Sau96I GGNCC 2 cut(s) 172, 332
ScrFI CCNGG 1 cut(s) 170
SduI GDGCHC 3 cut(s) 28, 344, 365
SetI ASST 4 cut(s) 155, 168, 362, 465
SfaNI GCATC 4 cut(s) 53, 102, 293, 382
SmiMI CAYNNNNRTG 2 cut(s) 254, 294
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
SsiI CCGC 4 cut(s) 31, 44, 71, 355
SspMI CTAG 2 cut(s) 210, 221
StyD4I CCNGG 1 cut(s) 168
TaaI ACNGT 3 cut(s) 17, 146, 451
TaiI ACGT 1 cut(s) 362
TaqI TCGA 1 cut(s) 374
TatI WGTACW 2 cut(s) 12, 258
TscAI CASTG 2 cut(s) 90, 204
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 3 cut(s) 90, 130, 413
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 3 cut(s) 25, 312, 389
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 2 cut(s) 90, 204
VneI GTGCAC 1 cut(s) 340
XceI RCATGY 1 cut(s) 259
XspI CTAG 2 cut(s) 210, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.