Rh1CG345800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0013832
Physical Location & Seq
Forward (+)
62331581 .. 62333215
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG345800.1

Sequence Viewer

Length: 888 bp
ATGTTCACACTACTATTGGCCACCTTGATAATCCTACCGCCATCTCCGATCAGTGCCTCCTCTCCATCTGTTTCTGCTCTCTTCGCTTTCGGCGATTCCACGGTGGATTCTGGCATGAACAACGACCTCATGCCTTTCGTTCGGTGCGACCATCCCCCTTACGGCAGAGACCTGCCGGAACACGTCCCCACCGGAAGGTACTCCAATGGAAAGATTGCAACTGACTTTATGGCCTCAAACTTGGGACTCAAGGACCTCTTGCCCGCTTACCTCGACCCGAAATTGACCGACTATGATTTGCTCACCGGGGTCAGTTTCGCGTCGGGAACTTGCGGCCTAGATGATCTGACCTTGGAGTTGAGCCACGTCTATAGTATGGGGGATCAGTTGGAGTATTTTGATGAAGCCGTAGTGAGGATGAAGATAAGTGTTGGGGAAAAGAAGGGTAGAGAGATTGTGGAAAATGCACTGTTTGTGGTTAGTGTTGGGACGAATGACTTGATGCTGAACGTTTATGAGTTTCCTCTAACCGAGAGGAAAACTCAGTTTACGTCTACAGACCAGTACTCTGATTTTCTGCTCCAGAGACTTGGGTCTTTTATTCAGGCTCTGTACAGTAGGGGAGCACGGCGGTTCATGGTGGCAGGTCTTCCACCGATCGGATGCCTTCCGCTTCAACGGACACTGAGCGGCATCATCAATCTGCGCCCTCAGCGCCTGTGCGTCACGCAGCAAAACATAGACTGTCAGACCTACAACTCCAAGCTCCAGGCCCTTCTCTCAAGTTTGCAAACCACACTACCACTCCCTAGTTCAAGGCTAGTCTATTTTGACATCTACAACCCCATCATGGACATGTACAACAACCCAGATAAATATGGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

295

Amino Acids

32.76

Weight (kDa)

4.98

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 27 - 284 5e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40250
fragaria_vesca FvH4_7g24850 FvH4_7g24850
malus_domestica MD01G1156400.v1.1 MD07G1225100.v1.1
prunus_persica Prupe.2G253600_v2.0.a1
pyrus_communis pycom07g20380
rosa_chinensis RchiOBHm_Chr1g0370731
rosa_laevigata RLG00000027015
rosa_multiflora Rmu_co8466073.1_g000001 Rmu_sc0004550.1_g000013
rosa_rugosa Rorug01G0358700
rosa_samantha Rh1AG368400 Rh1BG331900 Rh1CG345800 Rh1DG363300
rosa_wichuraiana Rw1G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 180, 635
AccBSI CCGCTC 1 cut(s) 690
AccI GTMKAC 1 cut(s) 554
AccII CGCG 1 cut(s) 320
AciI CCGC 6 cut(s) 38, 264, 333, 631, 671, 690
AclI AACGTT 1 cut(s) 510
AclWI GGATC 1 cut(s) 390
AcoI YGGCCR 1 cut(s) 18
AfaI GTAC 5 cut(s) 200, 566, 614, 860, 883
AfiI CCNNNNNNNGG 5 cut(s) 161, 195, 414, 659, 850
AflIII ACRYGT 2 cut(s) 181, 855
AgsI TTSAA 2 cut(s) 677, 816
AjiI CACGTC 2 cut(s) 184, 367
AjnI CCWGG 1 cut(s) 768
AluBI AGCT 1 cut(s) 766
AluI AGCT 1 cut(s) 766
Alw21I GWGCWC 1 cut(s) 628
Alw26I GTCTC 2 cut(s) 162, 580
AlwI GGATC 1 cut(s) 390
AlwNI CAGNNNCTG 2 cut(s) 610, 718
AoxI GGCC 4 cut(s) 18, 231, 334, 771
ApeKI GCWGC 1 cut(s) 730
AspLEI GCGC 2 cut(s) 708, 717
AspS9I GGNCC 2 cut(s) 253, 772
AsuC2I CCSGG 1 cut(s) 307
AsuHPI GGTGA 1 cut(s) 295
AvaII GGWCC 1 cut(s) 253
BalI TGGCCA 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 641
Bbv12I GWGCWC 1 cut(s) 628
BbvCI CCTCAGC 1 cut(s) 711
BbvI GCAGC 1 cut(s) 742
BccI CCATC 4 cut(s) 49, 73, 159, 854
BceAI ACGGC 3 cut(s) 178, 392, 644
BciT130I CCWGG 1 cut(s) 770
BcnI CCSGG 1 cut(s) 307
BcoDI GTCTC 2 cut(s) 162, 580
BfaI CTAG 3 cut(s) 338, 810, 821
BfmI CTRYAG 2 cut(s) 370, 555
BfoI RGCGCY 1 cut(s) 718
BfuAI ACCTGC 2 cut(s) 180, 635
BisI GCNGC 3 cut(s) 334, 691, 731
BlsI GCNGC 3 cut(s) 335, 692, 732
BmcAI AGTACT 1 cut(s) 566
Bme1390I CCNGG 2 cut(s) 307, 770
Bme18I GGWCC 1 cut(s) 253
BmgBI CACGTC 2 cut(s) 184, 367
BmgT120I GGNCC 2 cut(s) 253, 772
BmrFI CCNGG 2 cut(s) 307, 770
BmsI GCATC 3 cut(s) 492, 653, 702
BoxI GACNNNNGTC 1 cut(s) 592
BpiI GAAGAC 1 cut(s) 641
BplI GAGNNNNNCTC 2 cut(s) 526, 558
BpmI CTGGAG 2 cut(s) 566, 752
Bpu10I CCTNAGC 1 cut(s) 711
BpuEI CTTGAG 2 cut(s) 233, 766
BpuMI CCSGG 1 cut(s) 307
BsaI GGTCTC 1 cut(s) 162
BsaJI CCNNGG 3 cut(s) 99, 306, 351
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 2 cut(s) 789, 819
Bsc4I CCNNNNNNNGG 5 cut(s) 161, 195, 414, 659, 850
Bse1I ACTGG 1 cut(s) 562
BseBI CCWGG 1 cut(s) 770
BseDI CCNNGG 3 cut(s) 99, 306, 351
BseGI GGATG 3 cut(s) 151, 423, 668
BseLI CCNNNNNNNGG 5 cut(s) 161, 195, 414, 659, 850
BseMII CTCAG 3 cut(s) 557, 677, 725
BseNI ACTGG 1 cut(s) 562
BseRI GAGGAG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 742
Bsh1236I CGCG 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 660
BshFI GGCC 4 cut(s) 20, 233, 336, 773
BsiEI CGRYCG 1 cut(s) 660
BsiHKAI GWGCWC 1 cut(s) 628
BsiSI CCGG 3 cut(s) 176, 192, 306
BslFI GGGAC 3 cut(s) 170, 258, 502
BslI CCNNNNNNNGG 5 cut(s) 161, 195, 414, 659, 850
BsmAI GTCTC 2 cut(s) 162, 580
BsmFI GGGAC 3 cut(s) 170, 258, 502
BsnI GGCC 4 cut(s) 20, 233, 336, 773
Bso31I GGTCTC 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 628
Bsp1407I TGTACA 2 cut(s) 612, 858
Bsp143I GATC 4 cut(s) 48, 343, 382, 657
BspACI CCGC 6 cut(s) 38, 264, 333, 631, 671, 690
BspANI GGCC 4 cut(s) 20, 233, 336, 773
BspCNI CTCAG 3 cut(s) 556, 678, 724
BspFNI CGCG 1 cut(s) 320
BspMI ACCTGC 2 cut(s) 180, 635
BspPI GGATC 1 cut(s) 390
BspTNI GGTCTC 1 cut(s) 162
BsrBI CCGCTC 1 cut(s) 690
BsrGI TGTACA 2 cut(s) 612, 858
BsrI ACTGG 1 cut(s) 562
BssECI CCNNGG 3 cut(s) 99, 306, 351
BssMI GATC 4 cut(s) 48, 343, 382, 657
BssT1I CCWWGG 1 cut(s) 351
Bst2UI CCWGG 1 cut(s) 770
Bst4CI ACNGT 4 cut(s) 103, 471, 617, 746
Bst6I CTCTTC 1 cut(s) 86
BstAUI TGTACA 2 cut(s) 612, 858
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 3 cut(s) 543, 686, 711
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 3 cut(s) 151, 423, 668
BstFNI CGCG 1 cut(s) 320
BstH2I RGCGCY 1 cut(s) 718
BstHHI GCGC 2 cut(s) 708, 717
BstKTI GATC 4 cut(s) 51, 346, 385, 660
BstMAI GTCTC 2 cut(s) 162, 580
BstMBI GATC 4 cut(s) 48, 343, 382, 657
BstMCI CGRYCG 1 cut(s) 660
BstMWI GCNNNNNNNGC 3 cut(s) 83, 712, 714
BstNI CCWGG 1 cut(s) 770
BstNSI RCATGY 1 cut(s) 859
BstPAI GACNNNNGTC 1 cut(s) 592
BstSCI CCNGG 2 cut(s) 305, 768
BstSFI CTRYAG 2 cut(s) 370, 555
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 1 cut(s) 742
BstV2I GAAGAC 1 cut(s) 641
BstXI CCANNNNNNTGG 1 cut(s) 590
BsuRI GGCC 4 cut(s) 20, 233, 336, 773
BtgI CCRYGG 1 cut(s) 99
BtrI CACGTC 2 cut(s) 184, 367
BtsCI GGATG 3 cut(s) 151, 423, 668
BtsIMutI CAGTG 3 cut(s) 58, 467, 683
BveI ACCTGC 2 cut(s) 180, 635
Cac8I GCNNGC 1 cut(s) 264
CaiI CAGNNNCTG 2 cut(s) 610, 718
CfoI GCGC 2 cut(s) 708, 717
Cfr13I GGNCC 2 cut(s) 253, 772
CseI GACGC 2 cut(s) 309, 712
Csp6I GTAC 5 cut(s) 199, 565, 613, 859, 882
CviAII CATG 5 cut(s) 115, 130, 637, 850, 856
CviJI RGCY 9 cut(s) 20, 233, 336, 363, 407, 608, 766, 773, 820
CviKI_1 RGCY 9 cut(s) 20, 233, 336, 363, 407, 608, 766, 773, 820
CviQI GTAC 5 cut(s) 199, 565, 613, 859, 882
DdeI CTNAG 3 cut(s) 543, 686, 711
DpnI GATC 4 cut(s) 50, 345, 384, 659
DpnII GATC 4 cut(s) 48, 343, 382, 657
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
Eco130I CCWWGG 1 cut(s) 351
Eco31I GGTCTC 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 253
EcoO109I RGGNCCY 2 cut(s) 253, 772
EcoRII CCWGG 1 cut(s) 768
EcoT14I CCWWGG 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 5 cut(s) 118, 133, 640, 853, 859
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FaqI GGGAC 3 cut(s) 170, 258, 502
FatI CATG 5 cut(s) 114, 129, 636, 849, 855
FauI CCCGC 1 cut(s) 271
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 3 cut(s) 334, 691, 731
FokI GGATG 3 cut(s) 138, 430, 675
Fsp4HI GCNGC 3 cut(s) 334, 691, 731
FspBI CTAG 3 cut(s) 338, 810, 821
GlaI GCGC 2 cut(s) 707, 716
GluI GCNGC 3 cut(s) 334, 691, 731
GsuI CTGGAG 2 cut(s) 566, 752
HaeII RGCGCY 1 cut(s) 718
HaeIII GGCC 4 cut(s) 20, 233, 336, 773
HapII CCGG 3 cut(s) 176, 192, 306
HgaI GACGC 2 cut(s) 309, 712
HhaI GCGC 2 cut(s) 708, 717
Hin1II CATG 5 cut(s) 118, 133, 640, 853, 859
Hin6I GCGC 2 cut(s) 706, 715
HinP1I GCGC 2 cut(s) 706, 715
HinfI GANTC 3 cut(s) 95, 107, 246
HpaII CCGG 3 cut(s) 176, 192, 306
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 3 cut(s) 6, 549, 555
Hpy188I TCNGA 5 cut(s) 48, 348, 571, 662, 750
Hpy188III TCNNGA 2 cut(s) 324, 583
Hpy8I GTNNAC 3 cut(s) 6, 549, 555
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 4 cut(s) 189, 436, 677, 785
HpyCH4III ACNGT 4 cut(s) 103, 471, 617, 746
HpyCH4IV ACGT 4 cut(s) 183, 366, 510, 551
HpyCH4V TGCA 3 cut(s) 218, 467, 790
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 712, 714
HpyF3I CTNAG 3 cut(s) 543, 686, 711
HpySE526I ACGT 4 cut(s) 183, 366, 510, 551
Hsp92II CATG 5 cut(s) 118, 133, 640, 853, 859
HspAI GCGC 2 cut(s) 706, 715
Kzo9I GATC 4 cut(s) 48, 343, 382, 657
LmnI GCTCC 3 cut(s) 585, 623, 771
Lsp1109I GCAGC 1 cut(s) 742
LweI GCATC 3 cut(s) 492, 653, 702
MaeI CTAG 3 cut(s) 338, 810, 821
MaeII ACGT 4 cut(s) 183, 366, 510, 551
MaeIII GTNAC 1 cut(s) 724
MalI GATC 4 cut(s) 50, 345, 384, 659
MbiI CCGCTC 1 cut(s) 690
MboI GATC 4 cut(s) 48, 343, 382, 657
MboII GAAGA 3 cut(s) 73, 433, 641
MhlI GDGCHC 1 cut(s) 628
MlsI TGGCCA 1 cut(s) 20
MluCI AATT 1 cut(s) 281
MluNI TGGCCA 1 cut(s) 20
MlyI GAGTC 1 cut(s) 240
MmeI TCCRAC 1 cut(s) 369
Mox20I TGGCCA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 20
MslI CAYNNNNRTG 1 cut(s) 854
Msp20I TGGCCA 1 cut(s) 20
MspI CCGG 3 cut(s) 176, 192, 306
MspR9I CCNGG 2 cut(s) 307, 770
MvaI CCWGG 1 cut(s) 770
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 3 cut(s) 83, 712, 714
NciI CCSGG 1 cut(s) 307
NdeII GATC 4 cut(s) 48, 343, 382, 657
NlaIII CATG 5 cut(s) 118, 133, 640, 853, 859
NmuCI GTSAC 1 cut(s) 724
NspI RCATGY 1 cut(s) 859
PciI ACATGT 1 cut(s) 855
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 144
PfeI GAWTC 2 cut(s) 95, 107
PkrI GCNGC 3 cut(s) 335, 692, 732
Ple19I CGATCG 1 cut(s) 660
PleI GAGTC 1 cut(s) 240
PpsI GAGTC 1 cut(s) 240
PpuMI RGGWCCY 1 cut(s) 253
PscI ACATGT 1 cut(s) 855
PshAI GACNNNNGTC 1 cut(s) 592
Psp1406I AACGTT 1 cut(s) 510
Psp5II RGGWCCY 1 cut(s) 253
Psp6I CCWGG 1 cut(s) 768
PspGI CCWGG 1 cut(s) 768
PspPI GGNCC 2 cut(s) 253, 772
PspPPI RGGWCCY 1 cut(s) 253
PstNI CAGNNNCTG 2 cut(s) 610, 718
PvuI CGATCG 1 cut(s) 660
RsaI GTAC 5 cut(s) 200, 566, 614, 860, 883
RsaNI GTAC 5 cut(s) 199, 565, 613, 859, 882
RseI CAYNNNNRTG 1 cut(s) 854
SatI GCNGC 3 cut(s) 334, 691, 731
Sau3AI GATC 4 cut(s) 48, 343, 382, 657
Sau96I GGNCC 2 cut(s) 253, 772
ScaI AGTACT 1 cut(s) 566
SchI GAGTC 1 cut(s) 240
ScrFI CCNGG 2 cut(s) 307, 770
SduI GDGCHC 1 cut(s) 628
SfaNI GCATC 3 cut(s) 492, 653, 702
SfcI CTRYAG 2 cut(s) 370, 555
SinI GGWCC 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 854
SmlI CTYRAG 2 cut(s) 248, 781
SmoI CTYRAG 2 cut(s) 248, 781
Sse9I AATT 1 cut(s) 281
SsiI CCGC 6 cut(s) 38, 264, 333, 631, 671, 690
SspMI CTAG 3 cut(s) 338, 810, 821
StyD4I CCNGG 2 cut(s) 305, 768
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 4 cut(s) 103, 471, 617, 746
TaiI ACGT 4 cut(s) 186, 369, 513, 554
TaqI TCGA 1 cut(s) 273
TaqII GACCGA 1 cut(s) 302
TasI AATT 1 cut(s) 281
TatI WGTACW 3 cut(s) 564, 612, 858
TauI GCSGC 2 cut(s) 336, 693
TfiI GAWTC 2 cut(s) 95, 107
TscAI CASTG 3 cut(s) 58, 474, 690
TseFI GTSAC 1 cut(s) 724
TseI GCWGC 1 cut(s) 730
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 4 cut(s) 131, 417, 434, 625
TspGWI ACGGA 1 cut(s) 694
TspRI CASTG 3 cut(s) 58, 474, 690
VpaK11BI GGWCC 1 cut(s) 253
XceI RCATGY 1 cut(s) 859
XmiI GTMKAC 1 cut(s) 554
XspI CTAG 3 cut(s) 338, 810, 821
ZrmI AGTACT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.