AT2G40690

Glycerol-3-phosphate dehydrogenase NAD( )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010329
Physical Location & Seq
Forward (+)
16973992 .. 16976534
2543 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G40690.1

Sequence Viewer

Length: 1263 bp
ATGGCGGCTTCGGTGCAACCTGCATGCTTAGACCTCCACTTCTCCGGAAAGCATCCACCGCTTCTTAAACACAACGCTATTATCGTCCGCTGCGTTTCTTCTCCAAATGTAATTCCCGAAGCTGACTCCATCTCTGGTCCGCCTGATATCATCAATACGAACCGTGACCAGCGCAAAGTGGTTCGTATCGCTTGGGAGAAGTTGGTTCGATGGTCTCGTTCTTTGCGCGCTAAAGCTAAAACCGATGTTCTTGAGCGTACTCGCAAGGTTGTTGTTCTTGGTGGAGGTTCGTTTGGTACTGCAATGGCTGCTCATGTAGCTAGAAGGAAAGAGGGATTAGAGGTTAATATGCTTGTTCGTGACTCTTTTGTTTGTCAATCTATCAACGAGAACCACCATAATTGTAAGTATTTTCCTGAGCACAAGTTACCTGAGAATGTGATTGCTACAACTGATGCGAAAGCTGCATTGCTTGATGCTGATTACTGCCTTCATGCCGTGCCTGTGCAGTTTAGCTCTTCGTTTCTAGAGGGAATTGCCGATTATGTCGATCCAGGATTGCCTTTTATATCTCTTAGCAAAGGTCTGGAGCTTAATACTCTTAGGATGATGTCTCAGATCATTCCCATTGCGCTTAAGAATCCCCGGCAACCTTTTGTTGCTCTTTCTGGCCCGTCATTTGCTCTGGAGCTGATGAACAATTTACCAACTGCAATGGTGGTTGCCTCAAAAGATAAGAAATTGGCCAATGCTGTTCAGCAGCTTCTTGCTTCTAGTTACTTGAGAATAAATACTTCCAGTGATGTTACAGGCGTGGAAATCGCCGGTGCCCTGAAGAATGTTCTAGCAATAGCTGCAGGAATTGTTGATGGAATGAATCTCGGTAACAACTCTATGGCAGCTCTTGTGTCCCAAGGTTGTTCAGAGATAAGATGGTTAGCCACAAAGATGGGTGCAAAGCCAACAACCATTACTGGTTTATCAGGAACTGGGGACATAATGCTTACGTGTTTTGTAAATCTTTCAAGAAACCGAACAGTTGGAGTCAGGTTAGGGTCAGGGGAGACACTAGATGACATACTAACCTCTATGAATCAGGTTGCAGAAGGTGTAGCAACAGCCGGGGCAGTGATAGCATTAGCACAGAAATACAATGTGAAACTGCCGGTTTTGACAGCCGTAGCTAAGATAATAGATAATGAACTGACCCCGACTAAGGCTGTTCTTGAGCTCATGAACCTTCCTCAGATTGAAGAAGTATGA

Protein Analysis

420

Amino Acids

45.09

Weight (kDa)

8.75

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 89 - 248 2e-41 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 268 - 408 4.4e-50 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
GPD_NAD_C_bact PF20618 328 - 394 6.9e-21 Bacterial GPD, NAD-dependent C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 827
AccII CGCG 1 cut(s) 228
AccIII TCCGGA 1 cut(s) 44
AciI CCGC 4 cut(s) 5, 59, 88, 140
AclWI GGATC 1 cut(s) 545
AcoI YGGCCR 1 cut(s) 744
AcuI CTGAAG 1 cut(s) 854
AfaI GTAC 2 cut(s) 259, 298
AfiI CCNNNNNNNGG 1 cut(s) 1216
AflII CTTAAG 1 cut(s) 635
AflIII ACRYGT 1 cut(s) 1007
AgsI TTSAA 2 cut(s) 1026, 1253
AjnI CCWGG 1 cut(s) 553
Alw21I GWGCWC 2 cut(s) 423, 1233
Alw26I GTCTC 3 cut(s) 219, 618, 1058
AlwI GGATC 1 cut(s) 545
AlwNI CAGNNNCTG 1 cut(s) 989
Aor13HI TCCGGA 1 cut(s) 44
AoxI GGCC 2 cut(s) 670, 744
ApeKI GCWGC 6 cut(s) 90, 308, 464, 760, 854, 899
AspLEI GCGC 4 cut(s) 174, 228, 230, 634
AspS9I GGNCC 2 cut(s) 137, 671
AsuC2I CCSGG 2 cut(s) 646, 1123
AvaII GGWCC 1 cut(s) 137
BaeGI GKGCMC 1 cut(s) 832
BalI TGGCCA 1 cut(s) 746
BanI GGYRCC 1 cut(s) 827
BanII GRGCYC 1 cut(s) 1233
Bbv12I GWGCWC 2 cut(s) 423, 1233
BbvI GCAGC 6 cut(s) 77, 295, 451, 772, 841, 911
BccI CCATC 5 cut(s) 137, 204, 863, 927, 943
BceAI ACGGC 2 cut(s) 482, 1163
BciT130I CCWGG 1 cut(s) 555
BcnI CCSGG 2 cut(s) 646, 1123
BcoDI GTCTC 3 cut(s) 219, 618, 1058
BfaI CTAG 5 cut(s) 321, 527, 774, 845, 1070
BfmI CTRYAG 1 cut(s) 855
BfrI CTTAAG 1 cut(s) 635
BfuAI ACCTGC 1 cut(s) 28
BisI GCNGC 7 cut(s) 6, 91, 309, 465, 761, 855, 900
BlsI GCNGC 7 cut(s) 7, 92, 310, 466, 762, 856, 901
Bme1390I CCNGG 3 cut(s) 555, 646, 1123
Bme18I GGWCC 1 cut(s) 137
BmgT120I GGNCC 2 cut(s) 137, 671
BmiI GGNNCC 1 cut(s) 829
BmrFI CCNGG 3 cut(s) 555, 646, 1123
BmrI ACTGGG 1 cut(s) 999
BmsI GCATC 3 cut(s) 61, 445, 466
BmuI ACTGGG 1 cut(s) 999
BpmI CTGGAG 2 cut(s) 608, 707
Bpu10I CCTNAGC 1 cut(s) 417
BpuEI CTTGAG 3 cut(s) 272, 802, 1247
BpuMI CCSGG 2 cut(s) 646, 1123
BsaAI YACGTR 1 cut(s) 1008
BsaI GGTCTC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 644, 913, 1122
BsaWI WCCGGW 1 cut(s) 44
Bsc4I CCNNNNNNNGG 1 cut(s) 1216
Bse118I RCCGGY 2 cut(s) 824, 1165
Bse1I ACTGG 3 cut(s) 798, 979, 994
Bse3DI GCAATG 4 cut(s) 309, 467, 627, 720
BseAI TCCGGA 1 cut(s) 44
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 3 cut(s) 644, 913, 1122
BseGI GGATG 2 cut(s) 52, 612
BseLI CCNNNNNNNGG 1 cut(s) 1216
BseMI GCAATG 4 cut(s) 309, 467, 627, 720
BseMII CTCAG 4 cut(s) 408, 423, 629, 1259
BseNI ACTGG 3 cut(s) 798, 979, 994
BsePI GCGCGC 1 cut(s) 226
BseSI GKGCMC 1 cut(s) 832
BseXI GCAGC 6 cut(s) 77, 295, 451, 772, 841, 911
BsgI GTGCAG 1 cut(s) 527
Bsh1236I CGCG 1 cut(s) 228
BshFI GGCC 2 cut(s) 672, 746
BshNI GGYRCC 1 cut(s) 827
BsiHKAI GWGCWC 2 cut(s) 423, 1233
BsiSI CCGG 5 cut(s) 45, 646, 825, 1122, 1166
BslFI GGGAC 2 cut(s) 895, 1007
BslI CCNNNNNNNGG 1 cut(s) 1216
BsmAI GTCTC 3 cut(s) 219, 618, 1058
BsmFI GGGAC 2 cut(s) 895, 1007
BsnI GGCC 2 cut(s) 672, 746
Bso31I GGTCTC 1 cut(s) 219
Bsp1286I GDGCHC 3 cut(s) 423, 832, 1233
Bsp13I TCCGGA 1 cut(s) 44
Bsp143I GATC 2 cut(s) 550, 618
BspACI CCGC 4 cut(s) 5, 59, 88, 140
BspANI GGCC 2 cut(s) 672, 746
BspCNI CTCAG 4 cut(s) 409, 424, 628, 1258
BspEI TCCGGA 1 cut(s) 44
BspFNI CGCG 1 cut(s) 228
BspHI TCATGA 1 cut(s) 1233
BspLI GGNNCC 1 cut(s) 829
BspMAI CTGCAG 1 cut(s) 859
BspMI ACCTGC 1 cut(s) 28
BspPI GGATC 1 cut(s) 545
BspQI GCTCTTC 1 cut(s) 523
BspT107I GGYRCC 1 cut(s) 827
BspTI CTTAAG 1 cut(s) 635
BspTNI GGTCTC 1 cut(s) 219
BsrDI GCAATG 4 cut(s) 309, 467, 627, 720
BsrFI RCCGGY 2 cut(s) 824, 1165
BsrI ACTGG 3 cut(s) 798, 979, 994
BssAI RCCGGY 2 cut(s) 824, 1165
BssECI CCNNGG 3 cut(s) 644, 913, 1122
BssHII GCGCGC 1 cut(s) 226
BssMI GATC 2 cut(s) 550, 618
BssT1I CCWWGG 1 cut(s) 913
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 2 cut(s) 164, 1039
Bst6I CTCTTC 1 cut(s) 523
BstAFI CTTAAG 1 cut(s) 635
BstAPI GCANNNNNTGC 2 cut(s) 308, 854
BstBAI YACGTR 1 cut(s) 1008
BstC8I GCNNGC 2 cut(s) 25, 228
BstDEI CTNAG 9 cut(s) 28, 417, 432, 575, 602, 615, 1185, 1215, 1245
BstF5I GGATG 2 cut(s) 52, 612
BstFNI CGCG 1 cut(s) 228
BstHHI GCGC 4 cut(s) 174, 228, 230, 634
BstKTI GATC 2 cut(s) 553, 621
BstMAI GTCTC 3 cut(s) 219, 618, 1058
BstMBI GATC 2 cut(s) 550, 618
BstMWI GCNNNNNNNGC 6 cut(s) 58, 308, 317, 464, 854, 1133
BstNI CCWGG 1 cut(s) 555
BstNSI RCATGY 1 cut(s) 27
BstSCI CCNGG 3 cut(s) 553, 644, 1121
BstSFI CTRYAG 1 cut(s) 855
BstSLI GKGCMC 1 cut(s) 832
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 6 cut(s) 77, 295, 451, 772, 841, 911
BstXI CCANNNNNNTGG 1 cut(s) 949
BsuRI GGCC 2 cut(s) 672, 746
BtsCI GGATG 2 cut(s) 52, 612
BtsI GCAGTG 1 cut(s) 1134
BtsIMutI CAGTG 2 cut(s) 805, 1134
BveI ACCTGC 1 cut(s) 28
Cac8I GCNNGC 2 cut(s) 25, 228
CaiI CAGNNNCTG 1 cut(s) 989
CciI TCATGA 1 cut(s) 1233
CfoI GCGC 4 cut(s) 174, 228, 230, 634
Cfr10I RCCGGY 2 cut(s) 824, 1165
Cfr13I GGNCC 2 cut(s) 137, 671
Csp6I GTAC 2 cut(s) 258, 297
CspCI CAANNNNNGTGG 2 cut(s) 383, 418
CviAII CATG 4 cut(s) 24, 314, 494, 1234
CviQI GTAC 2 cut(s) 258, 297
DdeI CTNAG 9 cut(s) 28, 417, 432, 575, 602, 615, 1185, 1215, 1245
DpnI GATC 2 cut(s) 552, 620
DpnII GATC 2 cut(s) 550, 618
EaeI YGGCCR 1 cut(s) 744
Eam1104I CTCTTC 1 cut(s) 523
EarI CTCTTC 1 cut(s) 523
EciI GGCGGA 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 1231
Eco130I CCWWGG 1 cut(s) 913
Eco24I GRGCYC 1 cut(s) 1233
Eco31I GGTCTC 1 cut(s) 219
Eco32I GATATC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 137
Eco53kI GAGCTC 1 cut(s) 1231
Eco57I CTGAAG 1 cut(s) 854
EcoICRI GAGCTC 1 cut(s) 1231
EcoRII CCWGG 1 cut(s) 553
EcoRV GATATC 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 913
EcoT38I GRGCYC 1 cut(s) 1233
ErhI CCWWGG 1 cut(s) 913
FaeI CATG 4 cut(s) 27, 317, 497, 1237
FaqI GGGAC 2 cut(s) 895, 1007
FatI CATG 4 cut(s) 23, 313, 493, 1233
Fnu4HI GCNGC 7 cut(s) 6, 91, 309, 465, 761, 855, 900
FokI GGATG 2 cut(s) 39, 619
FriOI GRGCYC 1 cut(s) 1233
Fsp4HI GCNGC 7 cut(s) 6, 91, 309, 465, 761, 855, 900
FspBI CTAG 5 cut(s) 321, 527, 774, 845, 1070
GlaI GCGC 4 cut(s) 173, 227, 229, 633
GluI GCNGC 7 cut(s) 6, 91, 309, 465, 761, 855, 900
GsuI CTGGAG 2 cut(s) 608, 707
HaeIII GGCC 2 cut(s) 672, 746
HapII CCGG 5 cut(s) 45, 646, 825, 1122, 1166
HhaI GCGC 4 cut(s) 174, 228, 230, 634
Hin1II CATG 4 cut(s) 27, 317, 497, 1237
Hin6I GCGC 4 cut(s) 172, 226, 228, 632
HinP1I GCGC 4 cut(s) 172, 226, 228, 632
HinfI GANTC 6 cut(s) 125, 362, 640, 877, 1044, 1093
HpaII CCGG 5 cut(s) 45, 646, 825, 1122, 1166
Hpy188I TCNGA 3 cut(s) 618, 925, 1248
HpyAV CCTTC 4 cut(s) 318, 500, 1100, 1250
HpyCH4III ACNGT 2 cut(s) 164, 1039
HpyCH4IV ACGT 1 cut(s) 1007
HpyCH4V TGCA 9 cut(s) 16, 23, 302, 467, 508, 713, 857, 956, 1103
HpyF10VI GCNNNNNNNGC 6 cut(s) 58, 308, 317, 464, 854, 1133
HpyF3I CTNAG 9 cut(s) 28, 417, 432, 575, 602, 615, 1185, 1215, 1245
HpySE526I ACGT 1 cut(s) 1007
Hsp92II CATG 4 cut(s) 27, 317, 497, 1237
HspAI GCGC 4 cut(s) 172, 226, 228, 632
Kpn2I TCCGGA 1 cut(s) 44
Kzo9I GATC 2 cut(s) 550, 618
LguI GCTCTTC 1 cut(s) 523
LmnI GCTCC 2 cut(s) 589, 688
Lsp1109I GCAGC 6 cut(s) 77, 295, 451, 772, 841, 911
LweI GCATC 3 cut(s) 61, 445, 466
MaeI CTAG 5 cut(s) 321, 527, 774, 845, 1070
MaeII ACGT 1 cut(s) 1007
MaeIII GTNAC 6 cut(s) 164, 359, 426, 776, 805, 884
MalI GATC 2 cut(s) 552, 620
MboI GATC 2 cut(s) 550, 618
MboII GAAGA 3 cut(s) 90, 510, 847
MhlI GDGCHC 3 cut(s) 423, 832, 1233
MlsI TGGCCA 1 cut(s) 746
MluCI AATT 6 cut(s) 111, 400, 534, 700, 740, 861
MluNI TGGCCA 1 cut(s) 746
MlyI GAGTC 3 cut(s) 119, 356, 1053
MmeI TCCRAC 1 cut(s) 1021
MnlI CCTC 8 cut(s) 44, 278, 325, 334, 523, 736, 1096, 1254
Mox20I TGGCCA 1 cut(s) 746
MroI TCCGGA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 746
MseI TTAA 4 cut(s) 66, 345, 594, 636
MslI CAYNNNNRTG 1 cut(s) 947
Msp20I TGGCCA 1 cut(s) 746
MspA1I CMGCKG 1 cut(s) 90
MspCI CTTAAG 1 cut(s) 635
MspI CCGG 5 cut(s) 45, 646, 825, 1122, 1166
MspR9I CCNGG 3 cut(s) 555, 646, 1123
MvaI CCWGG 1 cut(s) 555
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 6 cut(s) 58, 308, 317, 464, 854, 1133
NciI CCSGG 2 cut(s) 646, 1123
NdeII GATC 2 cut(s) 550, 618
NlaIII CATG 4 cut(s) 27, 317, 497, 1237
NlaIV GGNNCC 1 cut(s) 829
NmuCI GTSAC 2 cut(s) 164, 359
NspI RCATGY 1 cut(s) 27
PaeI GCATGC 1 cut(s) 27
PagI TCATGA 1 cut(s) 1233
PauI GCGCGC 1 cut(s) 226
PciSI GCTCTTC 1 cut(s) 523
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 90, 214
PfeI GAWTC 3 cut(s) 640, 877, 1093
PfoI TCCNGGA 1 cut(s) 553
PkrI GCNGC 7 cut(s) 7, 92, 310, 466, 762, 856, 901
PleI GAGTC 3 cut(s) 119, 356, 1052
PpsI GAGTC 3 cut(s) 119, 356, 1052
Ppu21I YACGTR 1 cut(s) 1008
Psp124BI GAGCTC 1 cut(s) 1233
Psp6I CCWGG 1 cut(s) 553
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 1 cut(s) 829
PspPI GGNCC 2 cut(s) 137, 671
PstI CTGCAG 1 cut(s) 859
PstNI CAGNNNCTG 1 cut(s) 989
PteI GCGCGC 1 cut(s) 226
RsaI GTAC 2 cut(s) 259, 298
RsaNI GTAC 2 cut(s) 258, 297
RseI CAYNNNNRTG 1 cut(s) 947
SacI GAGCTC 1 cut(s) 1233
SapI GCTCTTC 1 cut(s) 523
SaqAI TTAA 4 cut(s) 66, 345, 594, 636
SatI GCNGC 7 cut(s) 6, 91, 309, 465, 761, 855, 900
Sau3AI GATC 2 cut(s) 550, 618
Sau96I GGNCC 2 cut(s) 137, 671
SchI GAGTC 3 cut(s) 119, 356, 1053
ScrFI CCNGG 3 cut(s) 555, 646, 1123
SduI GDGCHC 3 cut(s) 423, 832, 1233
SfaNI GCATC 3 cut(s) 61, 445, 466
SfcI CTRYAG 1 cut(s) 855
SgrAI CRCCGGYG 1 cut(s) 824
SinI GGWCC 1 cut(s) 137
SmiMI CAYNNNNRTG 1 cut(s) 947
SmlI CTYRAG 4 cut(s) 251, 635, 781, 1226
SmoI CTYRAG 4 cut(s) 251, 635, 781, 1226
SphI GCATGC 1 cut(s) 27
Sse9I AATT 6 cut(s) 111, 400, 534, 700, 740, 861
SsiI CCGC 4 cut(s) 5, 59, 88, 140
SspMI CTAG 5 cut(s) 321, 527, 774, 845, 1070
SstI GAGCTC 1 cut(s) 1233
StyD4I CCNGG 3 cut(s) 553, 644, 1121
StyI CCWWGG 1 cut(s) 913
TaaI ACNGT 2 cut(s) 164, 1039
TaiI ACGT 1 cut(s) 1010
TaqI TCGA 2 cut(s) 208, 549
TasI AATT 6 cut(s) 111, 400, 534, 700, 740, 861
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 640, 877, 1093
Tru1I TTAA 4 cut(s) 66, 345, 594, 636
Tru9I TTAA 4 cut(s) 66, 345, 594, 636
TscAI CASTG 2 cut(s) 805, 1134
TseFI GTSAC 2 cut(s) 164, 359
TseI GCWGC 6 cut(s) 90, 308, 464, 760, 854, 899
Tsp45I GTSAC 2 cut(s) 164, 359
TspDTI ATGAA 6 cut(s) 482, 710, 890, 1106, 1215, 1250
TspRI CASTG 2 cut(s) 805, 1134
Vha464I CTTAAG 1 cut(s) 635
VpaK11BI GGWCC 1 cut(s) 137
XbaI TCTAGA 1 cut(s) 526
XceI RCATGY 1 cut(s) 27
XspI CTAG 5 cut(s) 321, 527, 774, 845, 1070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.