Rh1BG343500

Glycerol-3-phosphate dehydrogenase NAD( )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0010329
Physical Location & Seq
Forward (+)
47682185 .. 47684649
2465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG343500.1

Sequence Viewer

Length: 1314 bp
ATGGCGGCGCTGCTAGAGCCACCGCCACCATTTCTAAACCCACTCCTTCCTCCGAGCTCAAACCCCACCCGCCCCCTTCCCCACAATCTCAAATTCAAAAGATTCAACCTTTCTAACAACCCCACCCTCCTCCTCACCCTCCATTCCCAACCCACCACTTGCTGCGCCGCCGCTCCCCAACCCGAATTCCCCGACCCGACTTCCGAGCCGACCCGCGACCGCCGCCGAGCCGTCCGGCTGGCCTGGGAGAAGCTTGTCCGGTGGTCCCGGTCTTTGCGCTCCAAGAACAAGACAGATGTTCTTGAACGCACTAACAAGGTGGTGGTGCTCGGAGGTGGGTCTTTTGCGACGGCGATGGCGGCCCACGTTGCAAATAGAAAGGATCAGATGGAAGTTAACATGCTGGTGCGTGATGCTCAAGTTTGTCAGTCTATTAATGAGAACCATTATAATGGTAAGTACTTCCCAGAACATAAGCTACCGGAGAATGTGATTGCAACCACTGATGCCAAAGCTGCTTTGCTTAATGCTGACTATTGCCTCCATGCAGTCCCTGTGCAGTTCAGCGCATCGTTTCTTGAGGGAATTGCGGAATATGTATATCCAGAGCTGCCGTTCATATCTGTTAGCAAGGGTTTGGAGCTCAATACATTGAGGACAATGTCTCAGATCATCCCCCAAGCATTGAAGAATCCTCGCCAGCCTTTCATTGCGCTATCAGGGCCTTCTTTTGCTCTGGAGCTGATGAACAAATTACCAACAGCGATGGTGGTAGCGTCAAAAGACAAAAAATTGGCTAATGCAGTTCAGCAGTTACTAGCTTCTAGCTATCTAAGAATCAGCACTTCAAGTGATGTCACAGGGGTAGAAGTTACAGGTGCTCTCAAGAATGTGCTTGCAATAGCCGCAGGTATTGTGGATGGGATGAATCTTGGTAATAATTCTAAGGCCGCTCTTGTTTCACAGGGATGCTCAGAGATAAGATGGCTGGCAACTAAGATGGGTGCGAAGCCGACAACAATCACTGGTCTATCTGGAATTGGAGATATTATGCTTACATGTTTTGTAAACCTTTCTAGAAACAGAACAGTTGGGGTTCGTCTTGGATCAGGGGAGAAACTTGATGACATACTTGGTTCCATGAATCAGGTGGCAGAAGGTGTATCGACAGCAGGAGCAGTGATTGCGCTAGCGCAGAGATATAACATCAAGATGCCAGTTTTGACTGCAGTAGCTCGGATTATTGACAATGAACTGACCCCCAAGAAAGCTGTTTTCGAGCTGATGAGCCTCCCTCAGGTTGAAGAAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

437

Amino Acids

47.4

Weight (kDa)

9.29

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 106 - 265 4.5e-40 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 285 - 425 1.7e-51 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
GPD_NAD_C_bact PF20618 345 - 411 1.3e-21 Bacterial GPD, NAD-dependent C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
Acc36I ACCTGC 1 cut(s) 899
AccBSI CCGCTC 2 cut(s) 173, 953
AccII CGCG 1 cut(s) 216
AclWI GGATC 2 cut(s) 390, 1114
AcsI RAATTY 2 cut(s) 92, 185
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 1 cut(s) 1297
AflIII ACRYGT 1 cut(s) 1058
AgsI TTSAA 6 cut(s) 97, 106, 305, 688, 849, 1304
AjnI CCWGG 1 cut(s) 242
Alw21I GWGCWC 4 cut(s) 59, 330, 645, 883
Alw26I GTCTC 1 cut(s) 669
AlwI GGATC 2 cut(s) 390, 1114
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 4 cut(s) 240, 360, 722, 948
ApeKI GCWGC 4 cut(s) 10, 162, 515, 610
ApoI RAATTY 2 cut(s) 92, 185
AseI ATTAAT 1 cut(s) 435
AspLEI GCGC 7 cut(s) 10, 167, 279, 569, 715, 1189, 1195
AspS9I GGNCC 3 cut(s) 264, 361, 722
AsuC2I CCSGG 1 cut(s) 268
AsuHPI GGTGA 1 cut(s) 127
AsuNHI GCTAGC 1 cut(s) 1189
AvaII GGWCC 1 cut(s) 264
AxyI CCTNAGG 1 cut(s) 1296
BanII GRGCYC 2 cut(s) 59, 645
Bbv12I GWGCWC 4 cut(s) 59, 330, 645, 883
BbvI GCAGC 3 cut(s) 149, 502, 597
BccI CCATC 6 cut(s) 349, 382, 760, 914, 978, 994
BceAI ACGGC 3 cut(s) 215, 366, 598
BciT130I CCWGG 1 cut(s) 244
BcnI CCSGG 1 cut(s) 268
BcoDI GTCTC 1 cut(s) 669
BfaI CTAG 5 cut(s) 14, 818, 825, 1077, 1190
BfmI CTRYAG 1 cut(s) 1227
BfoI RGCGCY 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 899
BmcAI AGTACT 1 cut(s) 461
Bme1390I CCNGG 2 cut(s) 244, 268
Bme18I GGWCC 1 cut(s) 264
BmgT120I GGNCC 3 cut(s) 264, 361, 722
BmiI GGNNCC 2 cut(s) 266, 1138
BmrFI CCNGG 2 cut(s) 244, 268
BmsI GCATC 5 cut(s) 403, 496, 578, 959, 1203
BmtI GCTAGC 1 cut(s) 1193
BplI GAGNNNNNCTC 2 cut(s) 1279, 1311
BpmI CTGGAG 1 cut(s) 758
BpuEI CTTGAG 3 cut(s) 402, 599, 869
BpuMI CCSGG 1 cut(s) 268
BsaJI CCNNGG 1 cut(s) 243
BsaWI WCCGGW 2 cut(s) 258, 481
Bsc4I CCNNNNNNNGG 1 cut(s) 1297
Bse1I ACTGG 2 cut(s) 1030, 1217
Bse21I CCTNAGG 1 cut(s) 1296
Bse3DI GCAATG 1 cut(s) 708
BseBI CCWGG 1 cut(s) 244
BseDI CCNNGG 1 cut(s) 243
BseGI GGATG 4 cut(s) 672, 925, 930, 974
BseLI CCNNNNNNNGG 1 cut(s) 1297
BseMI GCAATG 1 cut(s) 708
BseMII CTCAG 3 cut(s) 680, 987, 1310
BseNI ACTGG 2 cut(s) 1030, 1217
BseRI GAGGAG 2 cut(s) 119, 122
BseXI GCAGC 3 cut(s) 149, 502, 597
BsgI GTGCAG 1 cut(s) 578
Bsh1236I CGCG 1 cut(s) 216
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 4 cut(s) 242, 362, 724, 950
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 4 cut(s) 59, 330, 645, 883
BsiSI CCGG 4 cut(s) 235, 259, 268, 482
BslFI GGGAC 2 cut(s) 250, 536
BslI CCNNNNNNNGG 1 cut(s) 1297
BsmAI GTCTC 1 cut(s) 669
BsmFI GGGAC 2 cut(s) 250, 536
BsnI GGCC 4 cut(s) 242, 362, 724, 950
Bsp1286I GDGCHC 4 cut(s) 59, 330, 645, 883
Bsp143I GATC 3 cut(s) 382, 669, 1106
BspANI GGCC 4 cut(s) 242, 362, 724, 950
BspCNI CTCAG 3 cut(s) 679, 986, 1309
BspFNI CGCG 1 cut(s) 216
BspLI GGNNCC 2 cut(s) 266, 1138
BspMAI CTGCAG 1 cut(s) 1231
BspMI ACCTGC 1 cut(s) 899
BspOI GCTAGC 1 cut(s) 1193
BspPI GGATC 2 cut(s) 390, 1114
BsrBI CCGCTC 2 cut(s) 173, 953
BsrDI GCAATG 1 cut(s) 708
BsrI ACTGG 2 cut(s) 1030, 1217
BssECI CCNNGG 1 cut(s) 243
BssMI GATC 3 cut(s) 382, 669, 1106
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 1 cut(s) 1090
BstAPI GCANNNNNTGC 1 cut(s) 1184
BstC8I GCNNGC 5 cut(s) 240, 701, 897, 990, 1191
BstDEI CTNAG 6 cut(s) 666, 833, 945, 973, 996, 1296
BstENI CCTNNNNNAGG 1 cut(s) 1295
BstF5I GGATG 4 cut(s) 672, 925, 930, 974
BstFNI CGCG 1 cut(s) 216
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 7 cut(s) 10, 167, 279, 569, 715, 1189, 1195
BstKTI GATC 3 cut(s) 385, 672, 1109
BstMAI GTCTC 1 cut(s) 669
BstMBI GATC 3 cut(s) 382, 669, 1106
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 8 cut(s) 16, 222, 359, 368, 515, 721, 905, 1184
BstNI CCWGG 1 cut(s) 244
BstNSI RCATGY 2 cut(s) 403, 1062
BstSCI CCNGG 2 cut(s) 242, 266
BstSFI CTRYAG 1 cut(s) 1227
BstUI CGCG 1 cut(s) 216
BstV1I GCAGC 3 cut(s) 149, 502, 597
BstXI CCANNNNNNTGG 1 cut(s) 452
Bsu36I CCTNAGG 1 cut(s) 1296
BsuRI GGCC 4 cut(s) 242, 362, 724, 950
BtgZI GCGATG 2 cut(s) 368, 779
BtsCI GGATG 4 cut(s) 672, 925, 930, 974
BtsI GCAGTG 1 cut(s) 1185
BtsIMutI CAGTG 3 cut(s) 501, 1023, 1185
BveI ACCTGC 1 cut(s) 899
Cac8I GCNNGC 5 cut(s) 240, 701, 897, 990, 1191
CaiI CAGNNNCTG 1 cut(s) 554
CfoI GCGC 7 cut(s) 10, 167, 279, 569, 715, 1189, 1195
Cfr13I GGNCC 3 cut(s) 264, 361, 722
CseI GACGC 1 cut(s) 765
Csp6I GTAC 1 cut(s) 460
CviAII CATG 4 cut(s) 400, 545, 1059, 1141
CviQI GTAC 1 cut(s) 460
DdeI CTNAG 6 cut(s) 666, 833, 945, 973, 996, 1296
DpnI GATC 3 cut(s) 384, 671, 1108
DpnII GATC 3 cut(s) 382, 669, 1106
Ecl136II GAGCTC 2 cut(s) 57, 643
Eco24I GRGCYC 2 cut(s) 59, 645
Eco47I GGWCC 1 cut(s) 264
Eco53kI GAGCTC 2 cut(s) 57, 643
Eco81I CCTNAGG 1 cut(s) 1296
EcoICRI GAGCTC 2 cut(s) 57, 643
EcoNI CCTNNNNNAGG 1 cut(s) 1295
EcoO109I RGGNCCY 1 cut(s) 722
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 242
EcoT38I GRGCYC 2 cut(s) 59, 645
FaeI CATG 4 cut(s) 403, 548, 1062, 1144
FaqI GGGAC 2 cut(s) 250, 536
FatI CATG 4 cut(s) 399, 544, 1058, 1140
FauI CCCGC 2 cut(s) 77, 221
FokI GGATG 4 cut(s) 659, 932, 937, 981
FriOI GRGCYC 2 cut(s) 59, 645
FspBI CTAG 5 cut(s) 14, 818, 825, 1077, 1190
GlaI GCGC 7 cut(s) 9, 166, 278, 568, 714, 1188, 1194
GsuI CTGGAG 1 cut(s) 758
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 4 cut(s) 242, 362, 724, 950
HapII CCGG 4 cut(s) 235, 259, 268, 482
HgaI GACGC 1 cut(s) 765
HhaI GCGC 7 cut(s) 10, 167, 279, 569, 715, 1189, 1195
Hin1II CATG 4 cut(s) 403, 548, 1062, 1144
Hin6I GCGC 7 cut(s) 8, 165, 277, 567, 713, 1187, 1193
HinP1I GCGC 7 cut(s) 8, 165, 277, 567, 713, 1187, 1193
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 5 cut(s) 102, 691, 837, 928, 1144
HpaI GTTAAC 1 cut(s) 397
HpaII CCGG 4 cut(s) 235, 259, 268, 482
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 2 cut(s) 397, 1069
Hpy188I TCNGA 8 cut(s) 54, 205, 332, 387, 669, 976, 1239, 1313
Hpy188III TCNNGA 8 cut(s) 302, 578, 605, 737, 886, 1035, 1077, 1210
Hpy8I GTNNAC 2 cut(s) 397, 1069
Hpy99I CGWCG 1 cut(s) 352
HpyAV CCTTC 4 cut(s) 56, 86, 735, 1151
HpyCH4III ACNGT 1 cut(s) 1090
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 7 cut(s) 371, 497, 548, 559, 803, 899, 1229
HpyF10VI GCNNNNNNNGC 8 cut(s) 16, 222, 359, 368, 515, 721, 905, 1184
HpyF3I CTNAG 6 cut(s) 666, 833, 945, 973, 996, 1296
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 4 cut(s) 403, 548, 1062, 1144
HspAI GCGC 7 cut(s) 8, 165, 277, 567, 713, 1187, 1193
KspAI GTTAAC 1 cut(s) 397
Kzo9I GATC 3 cut(s) 382, 669, 1106
LmnI GCTCC 5 cut(s) 178, 284, 640, 739, 1175
Lsp1109I GCAGC 3 cut(s) 149, 502, 597
LweI GCATC 5 cut(s) 403, 496, 578, 959, 1203
MaeI CTAG 5 cut(s) 14, 818, 825, 1077, 1190
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 3 cut(s) 813, 856, 871
MalI GATC 3 cut(s) 384, 671, 1108
MbiI CCGCTC 2 cut(s) 173, 953
MboI GATC 3 cut(s) 382, 669, 1106
MboII GAAGA 1 cut(s) 700
MhlI GDGCHC 4 cut(s) 59, 330, 645, 883
MluCI AATT 7 cut(s) 92, 185, 585, 752, 791, 940, 1038
MseI TTAA 3 cut(s) 396, 435, 525
MslI CAYNNNNRTG 4 cut(s) 404, 450, 967, 1211
MspI CCGG 4 cut(s) 235, 259, 268, 482
MspR9I CCNGG 2 cut(s) 244, 268
MvaI CCWGG 1 cut(s) 244
MvnI CGCG 1 cut(s) 216
MwoI GCNNNNNNNGC 8 cut(s) 16, 222, 359, 368, 515, 721, 905, 1184
NciI CCSGG 1 cut(s) 268
NdeII GATC 3 cut(s) 382, 669, 1106
NheI GCTAGC 1 cut(s) 1189
NlaIII CATG 4 cut(s) 403, 548, 1062, 1144
NlaIV GGNNCC 2 cut(s) 266, 1138
NmeAIII GCCGAG 1 cut(s) 251
NmuCI GTSAC 1 cut(s) 856
NspI RCATGY 2 cut(s) 403, 1062
PciI ACATGT 1 cut(s) 1058
PfeI GAWTC 5 cut(s) 102, 691, 837, 928, 1144
PflFI GACNNNGTC 1 cut(s) 661
PscI ACATGT 1 cut(s) 1058
PshBI ATTAAT 1 cut(s) 435
PsiI TTATAA 1 cut(s) 450
Psp124BI GAGCTC 2 cut(s) 59, 645
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 2 cut(s) 266, 1138
PspPI GGNCC 3 cut(s) 264, 361, 722
PsrI GAACNNNNNNTAC 2 cut(s) 462, 494
PstI CTGCAG 1 cut(s) 1231
PstNI CAGNNNCTG 1 cut(s) 554
PsyI GACNNNGTC 1 cut(s) 661
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
RseI CAYNNNNRTG 4 cut(s) 404, 450, 967, 1211
SacI GAGCTC 2 cut(s) 59, 645
SaqAI TTAA 3 cut(s) 396, 435, 525
Sau3AI GATC 3 cut(s) 382, 669, 1106
Sau96I GGNCC 3 cut(s) 264, 361, 722
ScaI AGTACT 1 cut(s) 461
ScrFI CCNGG 2 cut(s) 244, 268
SduI GDGCHC 4 cut(s) 59, 330, 645, 883
SfaNI GCATC 5 cut(s) 403, 496, 578, 959, 1203
SfcI CTRYAG 1 cut(s) 1227
SinI GGWCC 1 cut(s) 264
SmiMI CAYNNNNRTG 4 cut(s) 404, 450, 967, 1211
SmlI CTYRAG 3 cut(s) 417, 578, 884
SmoI CTYRAG 3 cut(s) 417, 578, 884
Sse9I AATT 7 cut(s) 92, 185, 585, 752, 791, 940, 1038
SspMI CTAG 5 cut(s) 14, 818, 825, 1077, 1190
SstI GAGCTC 2 cut(s) 59, 645
StyD4I CCNGG 2 cut(s) 242, 266
TaaI ACNGT 1 cut(s) 1090
TaiI ACGT 1 cut(s) 369
TaqI TCGA 2 cut(s) 1166, 1278
TasI AATT 7 cut(s) 92, 185, 585, 752, 791, 940, 1038
TatI WGTACW 1 cut(s) 459
TauI GCSGC 7 cut(s) 8, 170, 173, 225, 362, 908, 953
TfiI GAWTC 5 cut(s) 102, 691, 837, 928, 1144
Tru1I TTAA 3 cut(s) 396, 435, 525
Tru9I TTAA 3 cut(s) 396, 435, 525
TscAI CASTG 3 cut(s) 508, 1030, 1185
TseFI GTSAC 1 cut(s) 856
TseI GCWGC 4 cut(s) 10, 162, 515, 610
Tsp45I GTSAC 1 cut(s) 856
TspDTI ATGAA 6 cut(s) 607, 697, 761, 941, 1157, 1266
TspRI CASTG 3 cut(s) 508, 1030, 1185
Tth111I GACNNNGTC 1 cut(s) 661
VpaK11BI GGWCC 1 cut(s) 264
VspI ATTAAT 1 cut(s) 435
XagI CCTNNNNNAGG 1 cut(s) 1295
XapI RAATTY 2 cut(s) 92, 185
XbaI TCTAGA 1 cut(s) 1076
XceI RCATGY 2 cut(s) 403, 1062
XcmI CCANNNNNNNNNTGG 1 cut(s) 1147
XspI CTAG 5 cut(s) 14, 818, 825, 1077, 1190
ZrmI AGTACT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.