Rh1DG374700

Glycerol-3-phosphate dehydrogenase NAD( )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0010329
Physical Location & Seq
Forward (+)
59804991 .. 59809971
4981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG374700.1

Sequence Viewer

Length: 903 bp
ATGGCGGCGCTGCTAGAGCCACCGCCACCATTTCTAAACCCACTCCTTCCTCCGAGCTCAAACCCCACCCGCCCCCCTCCCCACAATCTCAAATTCAAAAGATTCAACCTTTCTAACAACCCCACCCTCCATTCCCAACCCACCACTTGCTGCGCCGCCGCTCCCCAACCCAAATTCCCCGACCCGACCCGCGACCGCCGCCGAGCCGTCCGGCTGGCCTGGGAGAAGCTTGTCCGGTGGTCCCGGTCGTTGCGCTCCAAGAACAAGACAGATGTTCTTGAACGCACTAACAAGGTGGTGGTGCTCGGAGGTGGGTCTTTTGCGACGGCGATGGCGGCCCACGTTGCAAATAGAAAGGATCAGATGGAAGTTAACATGCTGGTGCGTGACGCTCAAGTTTGTCAGTCTATTAATGAGAACCATTATAATGGTAAGTACTTCCCAGAACATAAGCTACCGGAGAATGTGATTGCAACCACTGATGCCAAAGCTGCTTTGCTTAATGCTGACTACTGCCTCCATGCAGTCCCTGTGCAGTTCAGCGCATCGTTTCTTGAGGGAATTGCGGAATATGTATATCCAGAGTTGCCGTTCATATCTGTTAGCAAGGGTTTGGAGCTCAATACATTGAGGACAATGTCTCAGATCATCCCCCAAGCATTGAAGAATCCTCGCCAGCCTTTCATTGCGCTATCAGGGCCTTCTTTTGCTCTGGAGCTGATGAACAAATTACCAACAGCGATGGTGGTAGCGTCAAAAGACAAAAAATTGGCTAATGCAGTTCAGCAGTTACTAGCTTCTAGCTATCTAAGAATCAGCACTTCAAGGGGTAGAAGTTACAGGTGCTCTCAAGAATGTGCTTGCAATAGCTGCAGGTATTGTGGATGGGATGAATCTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

300

Amino Acids

33.49

Weight (kDa)

9.51

Isoelectric Point (pI)

55.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 98 - 257 1.9e-40 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 426
Acc36I ACCTGC 1 cut(s) 864
AccBSI CCGCTC 1 cut(s) 161
AccII CGCG 1 cut(s) 192
AclWI GGATC 1 cut(s) 366
AcsI RAATTY 2 cut(s) 92, 173
AfaI GTAC 1 cut(s) 437
AgsI TTSAA 5 cut(s) 97, 106, 281, 664, 825
AjnI CCWGG 1 cut(s) 218
AluBI AGCT 9 cut(s) 57, 229, 454, 491, 619, 718, 797, 804, 870
AluI AGCT 9 cut(s) 57, 229, 454, 491, 619, 718, 797, 804, 870
Alw21I GWGCWC 4 cut(s) 59, 306, 621, 848
Alw26I GTCTC 1 cut(s) 645
AlwI GGATC 1 cut(s) 366
AlwNI CAGNNNCTG 1 cut(s) 530
AoxI GGCC 3 cut(s) 216, 336, 698
ApeKI GCWGC 4 cut(s) 10, 150, 491, 870
ApoI RAATTY 2 cut(s) 92, 173
AseI ATTAAT 1 cut(s) 411
AspLEI GCGC 5 cut(s) 10, 155, 255, 545, 691
AspS9I GGNCC 3 cut(s) 240, 337, 698
AsuC2I CCSGG 1 cut(s) 244
AvaII GGWCC 1 cut(s) 240
BanII GRGCYC 2 cut(s) 59, 621
Bbv12I GWGCWC 4 cut(s) 59, 306, 621, 848
BbvI GCAGC 3 cut(s) 137, 478, 857
BccI CCATC 4 cut(s) 325, 358, 736, 879
BceAI ACGGC 3 cut(s) 191, 342, 574
BciT130I CCWGG 1 cut(s) 220
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 645
BfaI CTAG 3 cut(s) 14, 794, 801
BfmI CTRYAG 1 cut(s) 871
BfoI RGCGCY 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 864
BisI GCNGC 9 cut(s) 6, 11, 151, 156, 159, 199, 336, 492, 871
BlsI GCNGC 9 cut(s) 7, 12, 152, 157, 160, 200, 337, 493, 872
BmcAI AGTACT 1 cut(s) 437
Bme1390I CCNGG 2 cut(s) 220, 244
Bme18I GGWCC 1 cut(s) 240
BmgT120I GGNCC 3 cut(s) 240, 337, 698
BmiI GGNNCC 1 cut(s) 242
BmrFI CCNGG 2 cut(s) 220, 244
BmsI GCATC 2 cut(s) 472, 554
BpmI CTGGAG 1 cut(s) 734
BpuEI CTTGAG 3 cut(s) 378, 575, 834
BpuMI CCSGG 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 219
BsaWI WCCGGW 2 cut(s) 234, 457
Bse3DI GCAATG 1 cut(s) 684
BseBI CCWGG 1 cut(s) 220
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 3 cut(s) 648, 890, 895
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 1 cut(s) 656
BseXI GCAGC 3 cut(s) 137, 478, 857
BsgI GTGCAG 1 cut(s) 554
Bsh1236I CGCG 1 cut(s) 192
Bsh1285I CGRYCG 2 cut(s) 196, 248
BshFI GGCC 3 cut(s) 218, 338, 700
BsiEI CGRYCG 2 cut(s) 196, 248
BsiHKAI GWGCWC 4 cut(s) 59, 306, 621, 848
BsiSI CCGG 4 cut(s) 211, 235, 244, 458
BslFI GGGAC 2 cut(s) 226, 512
BsmAI GTCTC 1 cut(s) 645
BsmFI GGGAC 2 cut(s) 226, 512
BsnI GGCC 3 cut(s) 218, 338, 700
Bsp1286I GDGCHC 4 cut(s) 59, 306, 621, 848
Bsp143I GATC 2 cut(s) 358, 645
BspANI GGCC 3 cut(s) 218, 338, 700
BspCNI CTCAG 1 cut(s) 655
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 242
BspMAI CTGCAG 1 cut(s) 875
BspMI ACCTGC 1 cut(s) 864
BspPI GGATC 1 cut(s) 366
BsrBI CCGCTC 1 cut(s) 161
BsrDI GCAATG 1 cut(s) 684
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 2 cut(s) 358, 645
Bst2UI CCWGG 1 cut(s) 220
BstAPI GCANNNNNTGC 1 cut(s) 870
BstC8I GCNNGC 3 cut(s) 216, 677, 862
BstDEI CTNAG 2 cut(s) 642, 809
BstF5I GGATG 3 cut(s) 648, 890, 895
BstFNI CGCG 1 cut(s) 192
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 5 cut(s) 10, 155, 255, 545, 691
BstKTI GATC 2 cut(s) 361, 648
BstMAI GTCTC 1 cut(s) 645
BstMBI GATC 2 cut(s) 358, 645
BstMCI CGRYCG 2 cut(s) 196, 248
BstMWI GCNNNNNNNGC 7 cut(s) 16, 198, 335, 344, 491, 697, 870
BstNI CCWGG 1 cut(s) 220
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 2 cut(s) 218, 242
BstSFI CTRYAG 1 cut(s) 871
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 3 cut(s) 137, 478, 857
BstXI CCANNNNNNTGG 1 cut(s) 428
BsuRI GGCC 3 cut(s) 218, 338, 700
BtgZI GCGATG 2 cut(s) 344, 755
BtsCI GGATG 3 cut(s) 648, 890, 895
BtsIMutI CAGTG 1 cut(s) 477
BveI ACCTGC 1 cut(s) 864
Cac8I GCNNGC 3 cut(s) 216, 677, 862
CaiI CAGNNNCTG 1 cut(s) 530
CfoI GCGC 5 cut(s) 10, 155, 255, 545, 691
Cfr13I GGNCC 3 cut(s) 240, 337, 698
CseI GACGC 2 cut(s) 398, 741
Csp6I GTAC 1 cut(s) 436
CviAII CATG 2 cut(s) 376, 521
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 2 cut(s) 642, 809
DpnI GATC 2 cut(s) 360, 647
DpnII GATC 2 cut(s) 358, 645
Ecl136II GAGCTC 2 cut(s) 57, 619
Eco24I GRGCYC 2 cut(s) 59, 621
Eco47I GGWCC 1 cut(s) 240
Eco53kI GAGCTC 2 cut(s) 57, 619
EcoICRI GAGCTC 2 cut(s) 57, 619
EcoO109I RGGNCCY 1 cut(s) 698
EcoRII CCWGG 1 cut(s) 218
EcoT38I GRGCYC 2 cut(s) 59, 621
FaeI CATG 2 cut(s) 379, 524
FaiI YATR 7 cut(s) 377, 426, 450, 522, 573, 577, 596
FaqI GGGAC 2 cut(s) 226, 512
FatI CATG 2 cut(s) 375, 520
FauI CCCGC 2 cut(s) 77, 197
Fnu4HI GCNGC 9 cut(s) 6, 11, 151, 156, 159, 199, 336, 492, 871
FokI GGATG 2 cut(s) 635, 897
FriOI GRGCYC 2 cut(s) 59, 621
Fsp4HI GCNGC 9 cut(s) 6, 11, 151, 156, 159, 199, 336, 492, 871
FspBI CTAG 3 cut(s) 14, 794, 801
GlaI GCGC 5 cut(s) 9, 154, 254, 544, 690
GluI GCNGC 9 cut(s) 6, 11, 151, 156, 159, 199, 336, 492, 871
GsuI CTGGAG 1 cut(s) 734
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 3 cut(s) 218, 338, 700
HapII CCGG 4 cut(s) 211, 235, 244, 458
HgaI GACGC 2 cut(s) 398, 741
HhaI GCGC 5 cut(s) 10, 155, 255, 545, 691
Hin1II CATG 2 cut(s) 379, 524
Hin6I GCGC 5 cut(s) 8, 153, 253, 543, 689
HinP1I GCGC 5 cut(s) 8, 153, 253, 543, 689
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 4 cut(s) 102, 667, 813, 893
HpaI GTTAAC 1 cut(s) 373
HpaII CCGG 4 cut(s) 211, 235, 244, 458
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 4 cut(s) 54, 308, 363, 645
Hpy188III TCNNGA 5 cut(s) 278, 554, 581, 713, 851
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 2 cut(s) 56, 711
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 7 cut(s) 347, 473, 524, 535, 779, 864, 873
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 198, 335, 344, 491, 697, 870
HpyF3I CTNAG 2 cut(s) 642, 809
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 2 cut(s) 379, 524
HspAI GCGC 5 cut(s) 8, 153, 253, 543, 689
KspAI GTTAAC 1 cut(s) 373
Kzo9I GATC 2 cut(s) 358, 645
LmnI GCTCC 4 cut(s) 166, 260, 616, 715
Lsp1109I GCAGC 3 cut(s) 137, 478, 857
LweI GCATC 2 cut(s) 472, 554
MaeI CTAG 3 cut(s) 14, 794, 801
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 3 cut(s) 386, 789, 836
MalI GATC 2 cut(s) 360, 647
MbiI CCGCTC 1 cut(s) 161
MboI GATC 2 cut(s) 358, 645
MboII GAAGA 1 cut(s) 676
MhlI GDGCHC 4 cut(s) 59, 306, 621, 848
MluCI AATT 5 cut(s) 92, 173, 561, 728, 767
MnlI CCTC 8 cut(s) 60, 87, 137, 302, 527, 550, 624, 681
MseI TTAA 3 cut(s) 372, 411, 501
MslI CAYNNNNRTG 2 cut(s) 380, 426
MspI CCGG 4 cut(s) 211, 235, 244, 458
MspR9I CCNGG 2 cut(s) 220, 244
MvaI CCWGG 1 cut(s) 220
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 7 cut(s) 16, 198, 335, 344, 491, 697, 870
NciI CCSGG 1 cut(s) 244
NdeII GATC 2 cut(s) 358, 645
NlaIII CATG 2 cut(s) 379, 524
NlaIV GGNNCC 1 cut(s) 242
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 1 cut(s) 386
NspI RCATGY 1 cut(s) 379
PfeI GAWTC 4 cut(s) 102, 667, 813, 893
PflFI GACNNNGTC 1 cut(s) 637
PkrI GCNGC 9 cut(s) 7, 12, 152, 157, 160, 200, 337, 493, 872
PshBI ATTAAT 1 cut(s) 411
PsiI TTATAA 1 cut(s) 426
Psp124BI GAGCTC 2 cut(s) 59, 621
Psp6I CCWGG 1 cut(s) 218
PspGI CCWGG 1 cut(s) 218
PspN4I GGNNCC 1 cut(s) 242
PspPI GGNCC 3 cut(s) 240, 337, 698
PsrI GAACNNNNNNTAC 2 cut(s) 438, 470
PstI CTGCAG 1 cut(s) 875
PstNI CAGNNNCTG 1 cut(s) 530
PsyI GACNNNGTC 1 cut(s) 637
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
RseI CAYNNNNRTG 2 cut(s) 380, 426
SacI GAGCTC 2 cut(s) 59, 621
SaqAI TTAA 3 cut(s) 372, 411, 501
SatI GCNGC 9 cut(s) 6, 11, 151, 156, 159, 199, 336, 492, 871
Sau3AI GATC 2 cut(s) 358, 645
Sau96I GGNCC 3 cut(s) 240, 337, 698
ScaI AGTACT 1 cut(s) 437
ScrFI CCNGG 2 cut(s) 220, 244
SduI GDGCHC 4 cut(s) 59, 306, 621, 848
SfaNI GCATC 2 cut(s) 472, 554
SfcI CTRYAG 1 cut(s) 871
SinI GGWCC 1 cut(s) 240
SmiMI CAYNNNNRTG 2 cut(s) 380, 426
SmlI CTYRAG 3 cut(s) 393, 554, 849
SmoI CTYRAG 3 cut(s) 393, 554, 849
Sse9I AATT 5 cut(s) 92, 173, 561, 728, 767
SspMI CTAG 3 cut(s) 14, 794, 801
SstI GAGCTC 2 cut(s) 59, 621
StyD4I CCNGG 2 cut(s) 218, 242
TaiI ACGT 1 cut(s) 345
TasI AATT 5 cut(s) 92, 173, 561, 728, 767
TatI WGTACW 1 cut(s) 435
TauI GCSGC 5 cut(s) 8, 158, 161, 201, 338
TfiI GAWTC 4 cut(s) 102, 667, 813, 893
Tru1I TTAA 3 cut(s) 372, 411, 501
Tru9I TTAA 3 cut(s) 372, 411, 501
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 4 cut(s) 10, 150, 491, 870
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 3 cut(s) 583, 673, 737
TspRI CASTG 1 cut(s) 484
Tth111I GACNNNGTC 1 cut(s) 637
VpaK11BI GGWCC 1 cut(s) 240
VspI ATTAAT 1 cut(s) 411
XapI RAATTY 2 cut(s) 92, 173
XceI RCATGY 1 cut(s) 379
XspI CTAG 3 cut(s) 14, 794, 801
ZrmI AGTACT 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.