RchiOBHm_Chr1g0372371

Glycerol-3-phosphate dehydrogenase NAD( )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0010329
Physical Location & Seq
Forward (+)
61102066 .. 61104888
2823 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59636

Sequence Viewer

Length: 1290 bp
ATGGCGGCGCTGCTAGAGCCACCGCCACCATTTCTAAACCCACTCTTTCCTCCGAGCTCAAACCCCACCCGCCCCCTTCCCCACAATCTCAAATTCAAAAGATTCAACCTTTATACCAACCCCACCCTCCATTCCCAACCCACCACTTGCTGCGCCGCCGCTCCCCAACCCGAATTCCCCGACCCGACCCGCGACCGCCGCCGAGCCGTCCGGCTGGCCTGGGAGAAGCTTGTCCGGTGGTCCCGGTCGTTGCGCTCCAAGAACAAGACAGATGTTCTTGAACGCACTAACAAGGTGGTGGTGCTCGGAGGTGGGTCTTTTGCGACGGCGATGGCGGCCCACGTTGCAGATAGAAAGGATCAGATGGAAGTTAACATGCTGGTGCGTGATGCTCAAGTTTGTCAGTCTATTAATGAGAACCATTATAATGGTAAGTACTTCCCAGAACATAAGCTACCGGAGAATGTGATTGCAACCACTGATGCCAAAGCTGCTTTGCTTAATGCTGACTATTGCCTCCATGCAGTCCCTGTGCAGTTCAGCGCATCGTTTCTTGAGGGAATTGCGGAATATGTATATCCAGAGTTGCCGTTCATATCTGTTAGCAAGGGTTTGGAGCTCAATACATTGAGGACAATGTCTCAGATCATCCCCCAAGCATTGAAGAATCCTCGCCAGCCTTTCATTGCGCTATCAGGGCCTTCTTTTGCTCTGGAGCTGATGAACAAATTACCAACAGCGATGGTGGTAGCGTCAAAAGACAAAAAATTGGCTAATGCAGTTCAGCAGTTACTAGCTTCTAGCTATCTAAGAATCAGCACTTCAAGTGATGTCACAGGGGTAGAAGTTACAGGTGCTCTCAAGAATGTGCTTGCAATAGCCGCAGGTATTGTGGATGGGATGAATCTTGGTAATAATTCTAAGGCCGCTCTTGTTTCACAGGGATGCTCAGAGATAAGATGGCTGGCAACTAAGATGGGTGCGAAGCCGACAACAATCACTGGTCTATCTGGAATTGGAGATATTATGCTTACATGTTTTGTAAACCTTTCTAGAAACAGAACAGTTGGGGTTCGTCTTGGATCAGGGGAGAAACTTGATGACATACTTGGTTCCATGAATCAGGTGGCAGAAGGTGTATCGACAGCAGGAGCAGTGATTGCGCTAGCGCAGAAATATAACGTCAAGATGCCAGTTTTGACTGCAGTAGCTCGGATTATTGACAATGAACTGACCCCCAAGAAAGCTGTTTTCGAGCTGATGAGCCTCCCTCAGGTTGAAGAAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

429

Amino Acids

46.6

Weight (kDa)

9.26

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 98 - 257 3.6e-40 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 277 - 417 8.7e-52 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
GPD_NAD_C_bact PF20618 337 - 403 7e-22 Bacterial GPD, NAD-dependent C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 426
Acc36I ACCTGC 1 cut(s) 875
AccBSI CCGCTC 2 cut(s) 161, 929
AccII CGCG 1 cut(s) 192
AclWI GGATC 2 cut(s) 366, 1090
AcsI RAATTY 2 cut(s) 92, 173
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 1 cut(s) 1273
AflIII ACRYGT 1 cut(s) 1034
AgsI TTSAA 6 cut(s) 97, 106, 281, 664, 825, 1280
AjnI CCWGG 1 cut(s) 218
Alw21I GWGCWC 4 cut(s) 59, 306, 621, 859
Alw26I GTCTC 1 cut(s) 645
AlwI GGATC 2 cut(s) 366, 1090
AlwNI CAGNNNCTG 1 cut(s) 530
AoxI GGCC 4 cut(s) 216, 336, 698, 924
ApeKI GCWGC 3 cut(s) 10, 150, 491
ApoI RAATTY 2 cut(s) 92, 173
AseI ATTAAT 1 cut(s) 411
AspLEI GCGC 7 cut(s) 10, 155, 255, 545, 691, 1165, 1171
AspS9I GGNCC 3 cut(s) 240, 337, 698
AsuC2I CCSGG 1 cut(s) 244
AsuNHI GCTAGC 1 cut(s) 1165
AvaII GGWCC 1 cut(s) 240
AxyI CCTNAGG 1 cut(s) 1272
BanII GRGCYC 2 cut(s) 59, 621
Bbv12I GWGCWC 4 cut(s) 59, 306, 621, 859
BbvI GCAGC 2 cut(s) 137, 478
BccI CCATC 6 cut(s) 325, 358, 736, 890, 954, 970
BceAI ACGGC 3 cut(s) 191, 342, 574
BciT130I CCWGG 1 cut(s) 220
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 645
BfaI CTAG 5 cut(s) 14, 794, 801, 1053, 1166
BfmI CTRYAG 1 cut(s) 1203
BfoI RGCGCY 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 875
BmcAI AGTACT 1 cut(s) 437
Bme1390I CCNGG 2 cut(s) 220, 244
Bme18I GGWCC 1 cut(s) 240
BmgT120I GGNCC 3 cut(s) 240, 337, 698
BmiI GGNNCC 2 cut(s) 242, 1114
BmrFI CCNGG 2 cut(s) 220, 244
BmsI GCATC 5 cut(s) 379, 472, 554, 935, 1179
BmtI GCTAGC 1 cut(s) 1169
BplI GAGNNNNNCTC 2 cut(s) 1255, 1287
BpmI CTGGAG 1 cut(s) 734
BpuEI CTTGAG 3 cut(s) 378, 575, 845
BpuMI CCSGG 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 219
BsaWI WCCGGW 2 cut(s) 234, 457
Bsc4I CCNNNNNNNGG 1 cut(s) 1273
Bse1I ACTGG 2 cut(s) 1006, 1193
Bse21I CCTNAGG 1 cut(s) 1272
Bse3DI GCAATG 1 cut(s) 684
BseBI CCWGG 1 cut(s) 220
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 4 cut(s) 648, 901, 906, 950
BseLI CCNNNNNNNGG 1 cut(s) 1273
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 3 cut(s) 656, 963, 1286
BseNI ACTGG 2 cut(s) 1006, 1193
BseXI GCAGC 2 cut(s) 137, 478
BsgI GTGCAG 1 cut(s) 554
Bsh1236I CGCG 1 cut(s) 192
Bsh1285I CGRYCG 2 cut(s) 196, 248
BshFI GGCC 4 cut(s) 218, 338, 700, 926
BsiEI CGRYCG 2 cut(s) 196, 248
BsiHKAI GWGCWC 4 cut(s) 59, 306, 621, 859
BsiSI CCGG 4 cut(s) 211, 235, 244, 458
BslFI GGGAC 2 cut(s) 226, 512
BslI CCNNNNNNNGG 1 cut(s) 1273
BsmAI GTCTC 1 cut(s) 645
BsmFI GGGAC 2 cut(s) 226, 512
BsnI GGCC 4 cut(s) 218, 338, 700, 926
Bsp1286I GDGCHC 4 cut(s) 59, 306, 621, 859
Bsp143I GATC 3 cut(s) 358, 645, 1082
BspANI GGCC 4 cut(s) 218, 338, 700, 926
BspCNI CTCAG 3 cut(s) 655, 962, 1285
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 2 cut(s) 242, 1114
BspMAI CTGCAG 1 cut(s) 1207
BspMI ACCTGC 1 cut(s) 875
BspOI GCTAGC 1 cut(s) 1169
BspPI GGATC 2 cut(s) 366, 1090
BsrBI CCGCTC 2 cut(s) 161, 929
BsrDI GCAATG 1 cut(s) 684
BsrI ACTGG 2 cut(s) 1006, 1193
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 3 cut(s) 358, 645, 1082
Bst2UI CCWGG 1 cut(s) 220
Bst4CI ACNGT 1 cut(s) 1066
BstAPI GCANNNNNTGC 1 cut(s) 1160
BstC8I GCNNGC 5 cut(s) 216, 677, 873, 966, 1167
BstDEI CTNAG 6 cut(s) 642, 809, 921, 949, 972, 1272
BstENI CCTNNNNNAGG 1 cut(s) 1271
BstF5I GGATG 4 cut(s) 648, 901, 906, 950
BstFNI CGCG 1 cut(s) 192
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 7 cut(s) 10, 155, 255, 545, 691, 1165, 1171
BstKTI GATC 3 cut(s) 361, 648, 1085
BstMAI GTCTC 1 cut(s) 645
BstMBI GATC 3 cut(s) 358, 645, 1082
BstMCI CGRYCG 2 cut(s) 196, 248
BstMWI GCNNNNNNNGC 8 cut(s) 16, 198, 335, 344, 491, 697, 881, 1160
BstNI CCWGG 1 cut(s) 220
BstNSI RCATGY 2 cut(s) 379, 1038
BstSCI CCNGG 2 cut(s) 218, 242
BstSFI CTRYAG 1 cut(s) 1203
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 137, 478
BstXI CCANNNNNNTGG 1 cut(s) 428
Bsu36I CCTNAGG 1 cut(s) 1272
BsuRI GGCC 4 cut(s) 218, 338, 700, 926
BtgZI GCGATG 2 cut(s) 344, 755
BtsCI GGATG 4 cut(s) 648, 901, 906, 950
BtsI GCAGTG 1 cut(s) 1161
BtsIMutI CAGTG 3 cut(s) 477, 999, 1161
BveI ACCTGC 1 cut(s) 875
Cac8I GCNNGC 5 cut(s) 216, 677, 873, 966, 1167
CaiI CAGNNNCTG 1 cut(s) 530
CfoI GCGC 7 cut(s) 10, 155, 255, 545, 691, 1165, 1171
Cfr13I GGNCC 3 cut(s) 240, 337, 698
CseI GACGC 1 cut(s) 741
Csp6I GTAC 1 cut(s) 436
CviAII CATG 4 cut(s) 376, 521, 1035, 1117
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 6 cut(s) 642, 809, 921, 949, 972, 1272
DpnI GATC 3 cut(s) 360, 647, 1084
DpnII GATC 3 cut(s) 358, 645, 1082
Ecl136II GAGCTC 2 cut(s) 57, 619
Eco24I GRGCYC 2 cut(s) 59, 621
Eco47I GGWCC 1 cut(s) 240
Eco53kI GAGCTC 2 cut(s) 57, 619
Eco81I CCTNAGG 1 cut(s) 1272
EcoICRI GAGCTC 2 cut(s) 57, 619
EcoNI CCTNNNNNAGG 1 cut(s) 1271
EcoO109I RGGNCCY 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 218
EcoT38I GRGCYC 2 cut(s) 59, 621
FaeI CATG 4 cut(s) 379, 524, 1038, 1120
FaqI GGGAC 2 cut(s) 226, 512
FatI CATG 4 cut(s) 375, 520, 1034, 1116
FauI CCCGC 2 cut(s) 77, 197
FokI GGATG 4 cut(s) 635, 908, 913, 957
FriOI GRGCYC 2 cut(s) 59, 621
FspBI CTAG 5 cut(s) 14, 794, 801, 1053, 1166
GlaI GCGC 7 cut(s) 9, 154, 254, 544, 690, 1164, 1170
GsuI CTGGAG 1 cut(s) 734
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 4 cut(s) 218, 338, 700, 926
HapII CCGG 4 cut(s) 211, 235, 244, 458
HgaI GACGC 1 cut(s) 741
HhaI GCGC 7 cut(s) 10, 155, 255, 545, 691, 1165, 1171
Hin1II CATG 4 cut(s) 379, 524, 1038, 1120
Hin6I GCGC 7 cut(s) 8, 153, 253, 543, 689, 1163, 1169
HinP1I GCGC 7 cut(s) 8, 153, 253, 543, 689, 1163, 1169
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 5 cut(s) 102, 667, 813, 904, 1120
HpaI GTTAAC 1 cut(s) 373
HpaII CCGG 4 cut(s) 211, 235, 244, 458
Hpy166II GTNNAC 2 cut(s) 373, 1045
Hpy188I TCNGA 7 cut(s) 54, 308, 363, 645, 952, 1215, 1289
Hpy188III TCNNGA 8 cut(s) 278, 554, 581, 713, 862, 1011, 1053, 1186
Hpy8I GTNNAC 2 cut(s) 373, 1045
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 3 cut(s) 86, 711, 1127
HpyCH4III ACNGT 1 cut(s) 1066
HpyCH4IV ACGT 2 cut(s) 342, 1182
HpyCH4V TGCA 7 cut(s) 347, 473, 524, 535, 779, 875, 1205
HpyF10VI GCNNNNNNNGC 8 cut(s) 16, 198, 335, 344, 491, 697, 881, 1160
HpyF3I CTNAG 6 cut(s) 642, 809, 921, 949, 972, 1272
HpySE526I ACGT 2 cut(s) 342, 1182
Hsp92II CATG 4 cut(s) 379, 524, 1038, 1120
HspAI GCGC 7 cut(s) 8, 153, 253, 543, 689, 1163, 1169
KspAI GTTAAC 1 cut(s) 373
Kzo9I GATC 3 cut(s) 358, 645, 1082
LmnI GCTCC 5 cut(s) 166, 260, 616, 715, 1151
Lsp1109I GCAGC 2 cut(s) 137, 478
LweI GCATC 5 cut(s) 379, 472, 554, 935, 1179
MaeI CTAG 5 cut(s) 14, 794, 801, 1053, 1166
MaeII ACGT 2 cut(s) 342, 1182
MaeIII GTNAC 3 cut(s) 789, 832, 847
MalI GATC 3 cut(s) 360, 647, 1084
MbiI CCGCTC 2 cut(s) 161, 929
MboI GATC 3 cut(s) 358, 645, 1082
MboII GAAGA 1 cut(s) 676
MhlI GDGCHC 4 cut(s) 59, 306, 621, 859
MluCI AATT 7 cut(s) 92, 173, 561, 728, 767, 916, 1014
MnlI CCTC 9 cut(s) 60, 137, 302, 527, 550, 624, 681, 1277, 1281
MseI TTAA 3 cut(s) 372, 411, 501
MslI CAYNNNNRTG 3 cut(s) 380, 426, 943
MspI CCGG 4 cut(s) 211, 235, 244, 458
MspR9I CCNGG 2 cut(s) 220, 244
MvaI CCWGG 1 cut(s) 220
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 8 cut(s) 16, 198, 335, 344, 491, 697, 881, 1160
NciI CCSGG 1 cut(s) 244
NdeII GATC 3 cut(s) 358, 645, 1082
NheI GCTAGC 1 cut(s) 1165
NlaIII CATG 4 cut(s) 379, 524, 1038, 1120
NlaIV GGNNCC 2 cut(s) 242, 1114
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 1 cut(s) 832
NspI RCATGY 2 cut(s) 379, 1038
PciI ACATGT 1 cut(s) 1034
PfeI GAWTC 5 cut(s) 102, 667, 813, 904, 1120
PflFI GACNNNGTC 1 cut(s) 637
PscI ACATGT 1 cut(s) 1034
PshBI ATTAAT 1 cut(s) 411
PsiI TTATAA 1 cut(s) 426
Psp124BI GAGCTC 2 cut(s) 59, 621
Psp6I CCWGG 1 cut(s) 218
PspGI CCWGG 1 cut(s) 218
PspN4I GGNNCC 2 cut(s) 242, 1114
PspPI GGNCC 3 cut(s) 240, 337, 698
PsrI GAACNNNNNNTAC 2 cut(s) 438, 470
PstI CTGCAG 1 cut(s) 1207
PstNI CAGNNNCTG 1 cut(s) 530
PsyI GACNNNGTC 1 cut(s) 637
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
RseI CAYNNNNRTG 3 cut(s) 380, 426, 943
SacI GAGCTC 2 cut(s) 59, 621
SaqAI TTAA 3 cut(s) 372, 411, 501
Sau3AI GATC 3 cut(s) 358, 645, 1082
Sau96I GGNCC 3 cut(s) 240, 337, 698
ScaI AGTACT 1 cut(s) 437
ScrFI CCNGG 2 cut(s) 220, 244
SduI GDGCHC 4 cut(s) 59, 306, 621, 859
SfaNI GCATC 5 cut(s) 379, 472, 554, 935, 1179
SfcI CTRYAG 1 cut(s) 1203
SinI GGWCC 1 cut(s) 240
SmiMI CAYNNNNRTG 3 cut(s) 380, 426, 943
SmlI CTYRAG 3 cut(s) 393, 554, 860
SmoI CTYRAG 3 cut(s) 393, 554, 860
Sse9I AATT 7 cut(s) 92, 173, 561, 728, 767, 916, 1014
SspMI CTAG 5 cut(s) 14, 794, 801, 1053, 1166
SstI GAGCTC 2 cut(s) 59, 621
StyD4I CCNGG 2 cut(s) 218, 242
TaaI ACNGT 1 cut(s) 1066
TaiI ACGT 2 cut(s) 345, 1185
TaqI TCGA 2 cut(s) 1142, 1254
TasI AATT 7 cut(s) 92, 173, 561, 728, 767, 916, 1014
TatI WGTACW 1 cut(s) 435
TauI GCSGC 7 cut(s) 8, 158, 161, 201, 338, 884, 929
TfiI GAWTC 5 cut(s) 102, 667, 813, 904, 1120
Tru1I TTAA 3 cut(s) 372, 411, 501
Tru9I TTAA 3 cut(s) 372, 411, 501
TscAI CASTG 3 cut(s) 484, 1006, 1161
TseFI GTSAC 1 cut(s) 832
TseI GCWGC 3 cut(s) 10, 150, 491
Tsp45I GTSAC 1 cut(s) 832
TspDTI ATGAA 6 cut(s) 583, 673, 737, 917, 1133, 1242
TspRI CASTG 3 cut(s) 484, 1006, 1161
Tth111I GACNNNGTC 1 cut(s) 637
VpaK11BI GGWCC 1 cut(s) 240
VspI ATTAAT 1 cut(s) 411
XagI CCTNNNNNAGG 1 cut(s) 1271
XapI RAATTY 2 cut(s) 92, 173
XbaI TCTAGA 1 cut(s) 1052
XceI RCATGY 2 cut(s) 379, 1038
XcmI CCANNNNNNNNNTGG 1 cut(s) 1123
XspI CTAG 5 cut(s) 14, 794, 801, 1053, 1166
ZrmI AGTACT 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.