RLG00000026903

Glycerol-3-phosphate dehydrogenase NAD( )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0010329
Physical Location & Seq
Reverse (-)
6871643 .. 6874103
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026903

Sequence Viewer

Length: 1302 bp
ATGGCGGCGCTGCTAGAGCCACCGCCACCATTTCTAAACCCACTCCTTCCTTCGAGCTCAAACCCCACCCGCCCCCTTCCCCACAATCTCAAATTCAAAAGATTCAACCTTTGTAACAACCCCACCCTCCTCCTCACCCTCCATTCCCAACCCACCACTTGCTGCGCCGCCACTCCCCAACCCGAATTCCCCGACCCGACCCGCGACCGCCGCCGAGCCGTCCGGCTGGCCTGGGAGAAGCTGGTCCGGTGGTCCCGGTCGTTGCGCTCCAAGAACAAGACAGATGTTCTTGAACGCACTAACAAGGTGGTGGTGCTCGGAGGTGGGTCTTTTGCGACGGCGATGGCGGCCCACGTTGCAAATAGAAAGGATCAGATGGAAGTTAACATGCTGGTGCGTGATGCTCAAGTTTGTCAGTCTATTAATGAGAACCATTATAATGGTAAGTACTTCCCAGAACATAAGCTACCGGAGAATGTGATTGCAACCACTGATGCCAAAGCTGCTTTGCTTAATGCTGACTATTGCCTCCATGCAGTCCCTGTGCAGTTCAGCGCATCGTTTCTTGAGGGAATTGCGGAATATGTATATCCAGAGTTGCCGTTCATATCTGTTAGCAAGGGTTTGGAGCTCAATACATTGAGGACAATGTCTCAGATCATCCCCCAAGCATTGAAGAATCCTCGCCAGCCTTTCATTGCGCTATCAGGGCCTTCTTTTGCTCTGGAGCTGATGAACAAATTACCAACAGCAATGGTGGTAGCGTCAAAAGACAAAAAATTGGCTAATGCAGTTCAGCAGTTACTAGCTACTAGCTATCTAAGAATCAGCACTTCAAGTGATGTCACAGGGGTAGAAGTTACAGGTGCTCTCAAGAATGTGCTTGCAATAGCCGCAGGTATTGTAGATGGGATGAATCTTGGTAATAATTCTAAGGCAGCTCTTGTTTCACAGGGATGCTCAGAGATAAGATGGCTGGCAACTAAGATGGGTGCGAAGCCGACAACAATTACTGGTCTATCTGGAATTGGAGACATTATGCTTACATGTTTTGTAAACCTTTCTAGAAACAGAACAGTTGGGGTTCGTCTTGGATCAGGGGAGAAACTTGATGACATACTTGGTTCCATGAATCAGGTGGCAGAAGGTGTATCGACAGCAGGAGCAGTGATTGCGCTAGCGCAGAAATATAACGTCAAGATGCCAGTTTTGACTGCAGTAGCTCGGATTATTGACAATGAACTGACCCCCAAGAAAGCTGTTTTCGAGCTGATGAGCCTCCCTCAGGTTGAAGAAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

434

Amino Acids

47.0

Weight (kDa)

9.3

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 102 - 261 4.5e-40 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 281 - 421 8.8e-52 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
GPD_NAD_C_bact PF20618 341 - 407 7.1e-22 Bacterial GPD, NAD-dependent C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 438
Acc36I ACCTGC 1 cut(s) 887
AccII CGCG 1 cut(s) 204
AclWI GGATC 2 cut(s) 378, 1102
AcsI RAATTY 2 cut(s) 92, 185
AfaI GTAC 1 cut(s) 449
AfiI CCNNNNNNNGG 1 cut(s) 1285
AflIII ACRYGT 1 cut(s) 1046
AgsI TTSAA 6 cut(s) 97, 106, 293, 676, 837, 1292
AjnI CCWGG 1 cut(s) 230
Alw21I GWGCWC 4 cut(s) 59, 318, 633, 871
Alw26I GTCTC 2 cut(s) 657, 1026
AlwI GGATC 2 cut(s) 378, 1102
AlwNI CAGNNNCTG 1 cut(s) 542
AoxI GGCC 3 cut(s) 228, 348, 710
ApeKI GCWGC 4 cut(s) 10, 162, 503, 938
ApoI RAATTY 2 cut(s) 92, 185
AseI ATTAAT 1 cut(s) 423
AspLEI GCGC 7 cut(s) 10, 167, 267, 557, 703, 1177, 1183
AspS9I GGNCC 4 cut(s) 244, 252, 349, 710
AsuC2I CCSGG 1 cut(s) 256
AsuHPI GGTGA 1 cut(s) 127
AsuNHI GCTAGC 1 cut(s) 1177
AvaII GGWCC 2 cut(s) 244, 252
AxyI CCTNAGG 1 cut(s) 1284
BanII GRGCYC 2 cut(s) 59, 633
Bbv12I GWGCWC 4 cut(s) 59, 318, 633, 871
BbvI GCAGC 3 cut(s) 149, 490, 950
BccI CCATC 5 cut(s) 337, 370, 902, 966, 982
BceAI ACGGC 3 cut(s) 203, 354, 586
BciT130I CCWGG 1 cut(s) 232
BcnI CCSGG 1 cut(s) 256
BcoDI GTCTC 2 cut(s) 657, 1026
BfaI CTAG 5 cut(s) 14, 806, 813, 1065, 1178
BfmI CTRYAG 1 cut(s) 1215
BfoI RGCGCY 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 887
BisI GCNGC 9 cut(s) 6, 11, 163, 168, 211, 348, 504, 894, 939
BlsI GCNGC 9 cut(s) 7, 12, 164, 169, 212, 349, 505, 895, 940
BmcAI AGTACT 1 cut(s) 449
Bme1390I CCNGG 2 cut(s) 232, 256
Bme18I GGWCC 2 cut(s) 244, 252
BmgT120I GGNCC 4 cut(s) 244, 252, 349, 710
BmiI GGNNCC 2 cut(s) 254, 1126
BmrFI CCNGG 2 cut(s) 232, 256
BmsI GCATC 5 cut(s) 391, 484, 566, 947, 1191
BmtI GCTAGC 1 cut(s) 1181
BplI GAGNNNNNCTC 2 cut(s) 1267, 1299
BpmI CTGGAG 1 cut(s) 746
BpuEI CTTGAG 3 cut(s) 390, 587, 857
BpuMI CCSGG 1 cut(s) 256
BsaJI CCNNGG 1 cut(s) 231
BsaWI WCCGGW 2 cut(s) 246, 469
Bsc4I CCNNNNNNNGG 1 cut(s) 1285
Bse1I ACTGG 2 cut(s) 1018, 1205
Bse21I CCTNAGG 1 cut(s) 1284
Bse3DI GCAATG 2 cut(s) 696, 759
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 3 cut(s) 660, 918, 962
BseLI CCNNNNNNNGG 1 cut(s) 1285
BseMI GCAATG 2 cut(s) 696, 759
BseMII CTCAG 3 cut(s) 668, 975, 1298
BseNI ACTGG 2 cut(s) 1018, 1205
BseRI GAGGAG 2 cut(s) 119, 122
BseXI GCAGC 3 cut(s) 149, 490, 950
BsgI GTGCAG 1 cut(s) 566
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 2 cut(s) 208, 260
BshFI GGCC 3 cut(s) 230, 350, 712
BsiEI CGRYCG 2 cut(s) 208, 260
BsiHKAI GWGCWC 4 cut(s) 59, 318, 633, 871
BsiSI CCGG 4 cut(s) 223, 247, 256, 470
BslFI GGGAC 2 cut(s) 238, 524
BslI CCNNNNNNNGG 1 cut(s) 1285
BsmAI GTCTC 2 cut(s) 657, 1026
BsmFI GGGAC 2 cut(s) 238, 524
BsnI GGCC 3 cut(s) 230, 350, 712
Bsp1286I GDGCHC 4 cut(s) 59, 318, 633, 871
Bsp143I GATC 3 cut(s) 370, 657, 1094
BspANI GGCC 3 cut(s) 230, 350, 712
BspCNI CTCAG 3 cut(s) 667, 974, 1297
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 2 cut(s) 254, 1126
BspMAI CTGCAG 1 cut(s) 1219
BspMI ACCTGC 1 cut(s) 887
BspOI GCTAGC 1 cut(s) 1181
BspPI GGATC 2 cut(s) 378, 1102
BsrDI GCAATG 2 cut(s) 696, 759
BsrI ACTGG 2 cut(s) 1018, 1205
BssECI CCNNGG 1 cut(s) 231
BssMI GATC 3 cut(s) 370, 657, 1094
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 1 cut(s) 1078
BstAPI GCANNNNNTGC 1 cut(s) 1172
BstC8I GCNNGC 5 cut(s) 228, 689, 885, 978, 1179
BstDEI CTNAG 6 cut(s) 654, 821, 933, 961, 984, 1284
BstENI CCTNNNNNAGG 1 cut(s) 1283
BstF5I GGATG 3 cut(s) 660, 918, 962
BstFNI CGCG 1 cut(s) 204
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 7 cut(s) 10, 167, 267, 557, 703, 1177, 1183
BstKTI GATC 3 cut(s) 373, 660, 1097
BstMAI GTCTC 2 cut(s) 657, 1026
BstMBI GATC 3 cut(s) 370, 657, 1094
BstMCI CGRYCG 2 cut(s) 208, 260
BstMWI GCNNNNNNNGC 8 cut(s) 16, 210, 347, 356, 503, 709, 893, 1172
BstNI CCWGG 1 cut(s) 232
BstNSI RCATGY 2 cut(s) 391, 1050
BstSCI CCNGG 2 cut(s) 230, 254
BstSFI CTRYAG 1 cut(s) 1215
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 3 cut(s) 149, 490, 950
BstXI CCANNNNNNTGG 1 cut(s) 440
Bsu36I CCTNAGG 1 cut(s) 1284
BsuRI GGCC 3 cut(s) 230, 350, 712
BtgZI GCGATG 1 cut(s) 356
BtsCI GGATG 3 cut(s) 660, 918, 962
BtsI GCAGTG 1 cut(s) 1173
BtsIMutI CAGTG 2 cut(s) 489, 1173
BveI ACCTGC 1 cut(s) 887
Cac8I GCNNGC 5 cut(s) 228, 689, 885, 978, 1179
CaiI CAGNNNCTG 1 cut(s) 542
CfoI GCGC 7 cut(s) 10, 167, 267, 557, 703, 1177, 1183
Cfr13I GGNCC 4 cut(s) 244, 252, 349, 710
CseI GACGC 1 cut(s) 753
Csp6I GTAC 1 cut(s) 448
CviAII CATG 4 cut(s) 388, 533, 1047, 1129
CviQI GTAC 1 cut(s) 448
DdeI CTNAG 6 cut(s) 654, 821, 933, 961, 984, 1284
DpnI GATC 3 cut(s) 372, 659, 1096
DpnII GATC 3 cut(s) 370, 657, 1094
Ecl136II GAGCTC 2 cut(s) 57, 631
Eco24I GRGCYC 2 cut(s) 59, 633
Eco47I GGWCC 2 cut(s) 244, 252
Eco53kI GAGCTC 2 cut(s) 57, 631
Eco81I CCTNAGG 1 cut(s) 1284
EcoICRI GAGCTC 2 cut(s) 57, 631
EcoNI CCTNNNNNAGG 1 cut(s) 1283
EcoO109I RGGNCCY 1 cut(s) 710
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 230
EcoT38I GRGCYC 2 cut(s) 59, 633
FaeI CATG 4 cut(s) 391, 536, 1050, 1132
FaqI GGGAC 2 cut(s) 238, 524
FatI CATG 4 cut(s) 387, 532, 1046, 1128
FauI CCCGC 2 cut(s) 77, 209
Fnu4HI GCNGC 9 cut(s) 6, 11, 163, 168, 211, 348, 504, 894, 939
FokI GGATG 3 cut(s) 647, 925, 969
FriOI GRGCYC 2 cut(s) 59, 633
Fsp4HI GCNGC 9 cut(s) 6, 11, 163, 168, 211, 348, 504, 894, 939
FspBI CTAG 5 cut(s) 14, 806, 813, 1065, 1178
GlaI GCGC 7 cut(s) 9, 166, 266, 556, 702, 1176, 1182
GluI GCNGC 9 cut(s) 6, 11, 163, 168, 211, 348, 504, 894, 939
GsuI CTGGAG 1 cut(s) 746
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 3 cut(s) 230, 350, 712
HapII CCGG 4 cut(s) 223, 247, 256, 470
HgaI GACGC 1 cut(s) 753
HhaI GCGC 7 cut(s) 10, 167, 267, 557, 703, 1177, 1183
Hin1II CATG 4 cut(s) 391, 536, 1050, 1132
Hin6I GCGC 7 cut(s) 8, 165, 265, 555, 701, 1175, 1181
HinP1I GCGC 7 cut(s) 8, 165, 265, 555, 701, 1175, 1181
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 5 cut(s) 102, 679, 825, 916, 1132
HpaI GTTAAC 1 cut(s) 385
HpaII CCGG 4 cut(s) 223, 247, 256, 470
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 2 cut(s) 385, 1057
Hpy188I TCNGA 6 cut(s) 320, 375, 657, 964, 1227, 1301
Hpy188III TCNNGA 8 cut(s) 290, 566, 593, 725, 874, 1023, 1065, 1198
Hpy8I GTNNAC 2 cut(s) 385, 1057
Hpy99I CGWCG 1 cut(s) 340
HpyAV CCTTC 5 cut(s) 56, 60, 86, 723, 1139
HpyCH4III ACNGT 1 cut(s) 1078
HpyCH4IV ACGT 2 cut(s) 354, 1194
HpyCH4V TGCA 7 cut(s) 359, 485, 536, 547, 791, 887, 1217
HpyF10VI GCNNNNNNNGC 8 cut(s) 16, 210, 347, 356, 503, 709, 893, 1172
HpyF3I CTNAG 6 cut(s) 654, 821, 933, 961, 984, 1284
HpySE526I ACGT 2 cut(s) 354, 1194
Hsp92II CATG 4 cut(s) 391, 536, 1050, 1132
HspAI GCGC 7 cut(s) 8, 165, 265, 555, 701, 1175, 1181
KspAI GTTAAC 1 cut(s) 385
Kzo9I GATC 3 cut(s) 370, 657, 1094
LmnI GCTCC 4 cut(s) 272, 628, 727, 1163
Lsp1109I GCAGC 3 cut(s) 149, 490, 950
LweI GCATC 5 cut(s) 391, 484, 566, 947, 1191
MaeI CTAG 5 cut(s) 14, 806, 813, 1065, 1178
MaeII ACGT 2 cut(s) 354, 1194
MaeIII GTNAC 4 cut(s) 113, 801, 844, 859
MalI GATC 3 cut(s) 372, 659, 1096
MboI GATC 3 cut(s) 370, 657, 1094
MboII GAAGA 1 cut(s) 688
MhlI GDGCHC 4 cut(s) 59, 318, 633, 871
MluCI AATT 8 cut(s) 92, 185, 573, 740, 779, 928, 1008, 1026
MseI TTAA 3 cut(s) 384, 423, 513
MslI CAYNNNNRTG 3 cut(s) 392, 438, 955
MspI CCGG 4 cut(s) 223, 247, 256, 470
MspR9I CCNGG 2 cut(s) 232, 256
MvaI CCWGG 1 cut(s) 232
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 8 cut(s) 16, 210, 347, 356, 503, 709, 893, 1172
NciI CCSGG 1 cut(s) 256
NdeII GATC 3 cut(s) 370, 657, 1094
NheI GCTAGC 1 cut(s) 1177
NlaIII CATG 4 cut(s) 391, 536, 1050, 1132
NlaIV GGNNCC 2 cut(s) 254, 1126
NmeAIII GCCGAG 1 cut(s) 239
NmuCI GTSAC 1 cut(s) 844
NspI RCATGY 2 cut(s) 391, 1050
PciI ACATGT 1 cut(s) 1046
PfeI GAWTC 5 cut(s) 102, 679, 825, 916, 1132
PflFI GACNNNGTC 1 cut(s) 649
PkrI GCNGC 9 cut(s) 7, 12, 164, 169, 212, 349, 505, 895, 940
PscI ACATGT 1 cut(s) 1046
PshBI ATTAAT 1 cut(s) 423
PsiI TTATAA 1 cut(s) 438
Psp124BI GAGCTC 2 cut(s) 59, 633
Psp6I CCWGG 1 cut(s) 230
PspGI CCWGG 1 cut(s) 230
PspN4I GGNNCC 2 cut(s) 254, 1126
PspPI GGNCC 4 cut(s) 244, 252, 349, 710
PsrI GAACNNNNNNTAC 2 cut(s) 450, 482
PstI CTGCAG 1 cut(s) 1219
PstNI CAGNNNCTG 1 cut(s) 542
PsyI GACNNNGTC 1 cut(s) 649
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
RseI CAYNNNNRTG 3 cut(s) 392, 438, 955
SacI GAGCTC 2 cut(s) 59, 633
SaqAI TTAA 3 cut(s) 384, 423, 513
SatI GCNGC 9 cut(s) 6, 11, 163, 168, 211, 348, 504, 894, 939
Sau3AI GATC 3 cut(s) 370, 657, 1094
Sau96I GGNCC 4 cut(s) 244, 252, 349, 710
ScaI AGTACT 1 cut(s) 449
ScrFI CCNGG 2 cut(s) 232, 256
SduI GDGCHC 4 cut(s) 59, 318, 633, 871
SfaNI GCATC 5 cut(s) 391, 484, 566, 947, 1191
SfcI CTRYAG 1 cut(s) 1215
SinI GGWCC 2 cut(s) 244, 252
SmiMI CAYNNNNRTG 3 cut(s) 392, 438, 955
SmlI CTYRAG 3 cut(s) 405, 566, 872
SmoI CTYRAG 3 cut(s) 405, 566, 872
Sse9I AATT 8 cut(s) 92, 185, 573, 740, 779, 928, 1008, 1026
SspMI CTAG 5 cut(s) 14, 806, 813, 1065, 1178
SstI GAGCTC 2 cut(s) 59, 633
StyD4I CCNGG 2 cut(s) 230, 254
TaaI ACNGT 1 cut(s) 1078
TaiI ACGT 2 cut(s) 357, 1197
TaqI TCGA 3 cut(s) 53, 1154, 1266
TasI AATT 8 cut(s) 92, 185, 573, 740, 779, 928, 1008, 1026
TatI WGTACW 1 cut(s) 447
TauI GCSGC 5 cut(s) 8, 170, 213, 350, 896
TfiI GAWTC 5 cut(s) 102, 679, 825, 916, 1132
Tru1I TTAA 3 cut(s) 384, 423, 513
Tru9I TTAA 3 cut(s) 384, 423, 513
TscAI CASTG 2 cut(s) 496, 1173
TseFI GTSAC 1 cut(s) 844
TseI GCWGC 4 cut(s) 10, 162, 503, 938
Tsp45I GTSAC 1 cut(s) 844
TspDTI ATGAA 6 cut(s) 595, 685, 749, 929, 1145, 1254
TspRI CASTG 2 cut(s) 496, 1173
Tth111I GACNNNGTC 1 cut(s) 649
VpaK11BI GGWCC 2 cut(s) 244, 252
VspI ATTAAT 1 cut(s) 423
XagI CCTNNNNNAGG 1 cut(s) 1283
XapI RAATTY 2 cut(s) 92, 185
XbaI TCTAGA 1 cut(s) 1064
XceI RCATGY 2 cut(s) 391, 1050
XcmI CCANNNNNNNNNTGG 1 cut(s) 1135
XspI CTAG 5 cut(s) 14, 806, 813, 1065, 1178
ZrmI AGTACT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.