FvH4_7g25990

Glycerol-3-phosphate dehydrogenase NAD( )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010329
Physical Location & Seq
Forward (+)
19765416 .. 19768525
3110 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25990.t1

Sequence Viewer

Length: 1311 bp
ATGGCGGCGCTGCTAGAGCCACCGCCACCATTTCTAAACCCACTCCTTCCTCCGAGCTCAAACCCCACTCGCCTTCTTCTTCCCCACAATCTCAAATTCAAAAGACTCAACCTTTATAACAACCCCACCCTTCATTCCCAGACCACCACTTGCTTCGCTGCCACTCCCCAACCCGAACCCGAATTCCCCGACCCCACTTCGGAACCGACCCGCGACCGCCGCCGCGCCGTCCGGCTTGCCTGGGAGAAGATTGTCCGGTGGTCCCGGTCGTTACGCTCCAAGACCAAGACAGACATTCTTGAACGGACTAACAAGGTGGTGGTGCTCGGAGGTGGGTCTTTCGCGACGGCAATGGCGGCGCACGTGGCGAATAGGAAGGATGAGCTGGAAGTTAACATGCTGGTGCGTGATGCTCAAGTTTGTCGGTCTATTAATCATAACCATTTTAATAGTAAGTACTTCCCGGAACACAAGCTACCGGAGAATGTGATTGCAACCACTGATGCCAAGGCTGCTCTGCTAAATGCTGACTATTGCCTTCATGCAGTGCCTGTGCAGTTCAGTGCCTTGTTTCTTGAGGGAATTGCAGAGTATGTATATCCAGAGTTGCCATTCATATCTGTTAGCAAGGGTTTAGAGCTCAATACGTTGAGGACAATGTCTCAGATCATCCCCCAAGCATTGAAGAATCCTCGTCAGCCTTTCATCGCGTTATCAGGGCCTTCTTTTGCTCTGGAGCTGATGAACAGGTTACCAACAGCAATGGTGGTAGCGTCTAAAGACAAAAAATTGGCAAATGCAGTTCAGCAGTTACTAGCTTCTAGCTATCTAAGAATCAGCACTTCAAGTGATGTCACAGGGGTAGAAGTTACAGGTGCTCTCAAGAATGTGCTCGCAATAGCCGCAGGTATTGTGGATGGGATGAATCTTGGTAATAATTCCAAGGCAGCTCTTGTTTCACAAGGATGCTCAGAGATAAGATGGCTGGCAACTAAGATGGGTGCAAAGCCGACAACAATCACTGGTCTATCAGGAATTGGAGACATTATGCTTACATGCTTTGTAAATCTTTCAAGAAACAGAACAGTTGGGGTTCGTCTTGGATCAGGGGAGAGACTTGATGACATACTTAGTTCCATGAATCAGGTGGCAGAAGGTGTATCAACAGCAGGAGCAGTGATAGCTCTAGCGCAAAAATATAACGTGAAGATGCCAGTTTTGACCGCAGTAGCTCGGATTATTGACAATGAACTGACCCCCAAGAAAGCTGTTTTTGAGCTGATGAGCCTCCCTCAGGTTGAAGAAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

437

Amino Acids

47.54

Weight (kDa)

9.35

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 105 - 264 2.5e-39 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 284 - 424 5.3e-52 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
GPD_NAD_C_bact PF20618 344 - 410 7.1e-22 Bacterial GPD, NAD-dependent C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 117
Acc36I ACCTGC 1 cut(s) 896
AccII CGCG 4 cut(s) 213, 225, 344, 710
AciI CCGC 9 cut(s) 5, 23, 211, 217, 220, 223, 356, 903, 1224
AclWI GGATC 1 cut(s) 1111
AcsI RAATTY 2 cut(s) 95, 182
AcvI CACGTG 1 cut(s) 364
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 2 cut(s) 199, 1294
AgsI TTSAA 6 cut(s) 100, 302, 685, 846, 1074, 1301
AjnI CCWGG 1 cut(s) 239
AloI GAACNNNNNNTCC 2 cut(s) 168, 200
Alw21I GWGCWC 5 cut(s) 59, 327, 642, 880, 894
Alw26I GTCTC 3 cut(s) 666, 1035, 1108
AlwI GGATC 1 cut(s) 1111
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 1 cut(s) 719
ApeKI GCWGC 4 cut(s) 10, 158, 512, 947
ApoI RAATTY 2 cut(s) 95, 182
AseI ATTAAT 1 cut(s) 432
AspLEI GCGC 4 cut(s) 10, 227, 361, 1192
AspS9I GGNCC 2 cut(s) 261, 719
AsuC2I CCSGG 2 cut(s) 265, 464
AvaII GGWCC 1 cut(s) 261
AxyI CCTNAGG 1 cut(s) 1293
BanII GRGCYC 2 cut(s) 59, 642
BbrPI CACGTG 1 cut(s) 364
Bbv12I GWGCWC 5 cut(s) 59, 327, 642, 880, 894
BbvI GCAGC 3 cut(s) 145, 499, 959
BccI CCATC 3 cut(s) 911, 975, 991
BceAI ACGGC 2 cut(s) 212, 363
BciT130I CCWGG 1 cut(s) 241
BcnI CCSGG 2 cut(s) 265, 464
BcoDI GTCTC 3 cut(s) 666, 1035, 1108
BfaI CTAG 4 cut(s) 14, 815, 822, 1187
BfoI RGCGCY 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 896
BisI GCNGC 9 cut(s) 6, 11, 159, 220, 223, 357, 513, 903, 948
BlsI GCNGC 9 cut(s) 7, 12, 160, 221, 224, 358, 514, 904, 949
BmcAI AGTACT 1 cut(s) 458
Bme1390I CCNGG 3 cut(s) 241, 265, 464
Bme18I GGWCC 1 cut(s) 261
BmgT120I GGNCC 2 cut(s) 261, 719
BmiI GGNNCC 2 cut(s) 204, 263
BmrFI CCNGG 3 cut(s) 241, 265, 464
BmsI GCATC 4 cut(s) 400, 493, 956, 1200
BplI GAGNNNNNCTC 2 cut(s) 1276, 1308
BpmI CTGGAG 1 cut(s) 755
BpuEI CTTGAG 3 cut(s) 399, 596, 866
BpuMI CCSGG 2 cut(s) 265, 464
BsaAI YACGTR 1 cut(s) 364
BsaJI CCNNGG 3 cut(s) 240, 507, 942
BsaWI WCCGGW 2 cut(s) 255, 478
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 1294
Bse1I ACTGG 2 cut(s) 1027, 1214
Bse21I CCTNAGG 1 cut(s) 1293
Bse3DI GCAATG 2 cut(s) 357, 768
BseBI CCWGG 1 cut(s) 241
BseDI CCNNGG 3 cut(s) 240, 507, 942
BseGI GGATG 5 cut(s) 385, 669, 922, 927, 971
BseLI CCNNNNNNNGG 2 cut(s) 199, 1294
BseMI GCAATG 2 cut(s) 357, 768
BseMII CTCAG 3 cut(s) 677, 984, 1307
BseNI ACTGG 2 cut(s) 1027, 1214
BseXI GCAGC 3 cut(s) 145, 499, 959
BsgI GTGCAG 1 cut(s) 575
Bsh1236I CGCG 4 cut(s) 213, 225, 344, 710
Bsh1285I CGRYCG 2 cut(s) 217, 269
BshFI GGCC 1 cut(s) 721
BsiEI CGRYCG 2 cut(s) 217, 269
BsiHKAI GWGCWC 5 cut(s) 59, 327, 642, 880, 894
BsiSI CCGG 5 cut(s) 232, 256, 265, 464, 479
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 2 cut(s) 199, 1294
BsmAI GTCTC 3 cut(s) 666, 1035, 1108
BsmFI GGGAC 1 cut(s) 247
BsnI GGCC 1 cut(s) 721
Bsp1286I GDGCHC 5 cut(s) 59, 327, 642, 880, 894
Bsp143I GATC 2 cut(s) 666, 1103
Bsp68I TCGCGA 1 cut(s) 344
BspACI CCGC 9 cut(s) 5, 23, 211, 217, 220, 223, 356, 903, 1224
BspANI GGCC 1 cut(s) 721
BspCNI CTCAG 3 cut(s) 676, 983, 1306
BspFNI CGCG 4 cut(s) 213, 225, 344, 710
BspLI GGNNCC 2 cut(s) 204, 263
BspMI ACCTGC 1 cut(s) 896
BspPI GGATC 1 cut(s) 1111
BsrDI GCAATG 2 cut(s) 357, 768
BsrI ACTGG 2 cut(s) 1027, 1214
BssECI CCNNGG 3 cut(s) 240, 507, 942
BssMI GATC 2 cut(s) 666, 1103
BssT1I CCWWGG 2 cut(s) 507, 942
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 1 cut(s) 1087
BstBAI YACGTR 1 cut(s) 364
BstC8I GCNNGC 3 cut(s) 237, 894, 987
BstDEI CTNAG 6 cut(s) 663, 830, 970, 993, 1130, 1293
BstEII GGTNACC 1 cut(s) 750
BstENI CCTNNNNNAGG 1 cut(s) 1292
BstF5I GGATG 5 cut(s) 385, 669, 922, 927, 971
BstFNI CGCG 4 cut(s) 213, 225, 344, 710
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 4 cut(s) 10, 227, 361, 1192
BstKTI GATC 2 cut(s) 669, 1106
BstMAI GTCTC 3 cut(s) 666, 1035, 1108
BstMBI GATC 2 cut(s) 666, 1103
BstMCI CGRYCG 2 cut(s) 217, 269
BstMWI GCNNNNNNNGC 7 cut(s) 16, 219, 356, 365, 512, 902, 1181
BstNI CCWGG 1 cut(s) 241
BstNSI RCATGY 2 cut(s) 400, 1059
BstPI GGTNACC 1 cut(s) 750
BstSCI CCNGG 3 cut(s) 239, 263, 462
BstUI CGCG 4 cut(s) 213, 225, 344, 710
BstV1I GCAGC 3 cut(s) 145, 499, 959
Bsu36I CCTNAGG 1 cut(s) 1293
BsuRI GGCC 1 cut(s) 721
BtgZI GCGATG 1 cut(s) 691
BtsCI GGATG 5 cut(s) 385, 669, 922, 927, 971
BtsI GCAGTG 2 cut(s) 552, 1182
BtsIMutI CAGTG 5 cut(s) 498, 552, 568, 1020, 1182
BtuMI TCGCGA 1 cut(s) 344
BveI ACCTGC 1 cut(s) 896
Cac8I GCNNGC 3 cut(s) 237, 894, 987
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 4 cut(s) 10, 227, 361, 1192
Cfr13I GGNCC 2 cut(s) 261, 719
CseI GACGC 1 cut(s) 762
Csp6I GTAC 1 cut(s) 457
CviAII CATG 4 cut(s) 397, 542, 1056, 1138
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 6 cut(s) 663, 830, 970, 993, 1130, 1293
DpnI GATC 2 cut(s) 668, 1105
DpnII GATC 2 cut(s) 666, 1103
Ecl136II GAGCTC 2 cut(s) 57, 640
Eco130I CCWWGG 2 cut(s) 507, 942
Eco24I GRGCYC 2 cut(s) 59, 642
Eco47I GGWCC 1 cut(s) 261
Eco53kI GAGCTC 2 cut(s) 57, 640
Eco72I CACGTG 1 cut(s) 364
Eco81I CCTNAGG 1 cut(s) 1293
Eco91I GGTNACC 1 cut(s) 750
EcoICRI GAGCTC 2 cut(s) 57, 640
EcoNI CCTNNNNNAGG 1 cut(s) 1292
EcoO109I RGGNCCY 1 cut(s) 719
EcoO65I GGTNACC 1 cut(s) 750
EcoRI GAATTC 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 239
EcoT14I CCWWGG 2 cut(s) 507, 942
EcoT38I GRGCYC 2 cut(s) 59, 642
ErhI CCWWGG 2 cut(s) 507, 942
FaeI CATG 4 cut(s) 400, 545, 1059, 1141
FaqI GGGAC 1 cut(s) 247
FatI CATG 4 cut(s) 396, 541, 1055, 1137
FauI CCCGC 1 cut(s) 218
Fnu4HI GCNGC 9 cut(s) 6, 11, 159, 220, 223, 357, 513, 903, 948
FokI GGATG 5 cut(s) 392, 656, 929, 934, 978
FriOI GRGCYC 2 cut(s) 59, 642
Fsp4HI GCNGC 9 cut(s) 6, 11, 159, 220, 223, 357, 513, 903, 948
FspBI CTAG 4 cut(s) 14, 815, 822, 1187
GlaI GCGC 4 cut(s) 9, 226, 360, 1191
GluI GCNGC 9 cut(s) 6, 11, 159, 220, 223, 357, 513, 903, 948
GsuI CTGGAG 1 cut(s) 755
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 1 cut(s) 721
HapII CCGG 5 cut(s) 232, 256, 265, 464, 479
HgaI GACGC 1 cut(s) 762
HhaI GCGC 4 cut(s) 10, 227, 361, 1192
Hin1II CATG 4 cut(s) 400, 545, 1059, 1141
Hin6I GCGC 4 cut(s) 8, 225, 359, 1190
HinP1I GCGC 4 cut(s) 8, 225, 359, 1190
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HinfI GANTC 5 cut(s) 105, 688, 834, 925, 1141
HpaI GTTAAC 1 cut(s) 394
HpaII CCGG 5 cut(s) 232, 256, 265, 464, 479
Hpy166II GTNNAC 1 cut(s) 394
Hpy188I TCNGA 7 cut(s) 54, 202, 329, 666, 973, 1236, 1310
Hpy188III TCNNGA 8 cut(s) 299, 343, 575, 602, 734, 883, 1032, 1074
Hpy8I GTNNAC 1 cut(s) 394
Hpy99I CGWCG 1 cut(s) 349
HpyAV CCTTC 7 cut(s) 56, 83, 140, 370, 548, 732, 1148
HpyCH4III ACNGT 1 cut(s) 1087
HpyCH4IV ACGT 3 cut(s) 363, 647, 1203
HpyCH4V TGCA 6 cut(s) 494, 545, 556, 587, 800, 1004
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 219, 356, 365, 512, 902, 1181
HpyF3I CTNAG 6 cut(s) 663, 830, 970, 993, 1130, 1293
HpySE526I ACGT 3 cut(s) 363, 647, 1203
Hsp92II CATG 4 cut(s) 400, 545, 1059, 1141
HspAI GCGC 4 cut(s) 8, 225, 359, 1190
KspAI GTTAAC 1 cut(s) 394
Kzo9I GATC 2 cut(s) 666, 1103
LmnI GCTCC 3 cut(s) 281, 736, 1172
Lsp1109I GCAGC 3 cut(s) 145, 499, 959
LweI GCATC 4 cut(s) 400, 493, 956, 1200
MaeI CTAG 4 cut(s) 14, 815, 822, 1187
MaeII ACGT 3 cut(s) 363, 647, 1203
MaeIII GTNAC 5 cut(s) 270, 750, 810, 853, 868
MalI GATC 2 cut(s) 668, 1105
MboI GATC 2 cut(s) 666, 1103
MboII GAAGA 5 cut(s) 68, 71, 259, 697, 1219
MhlI GDGCHC 5 cut(s) 59, 327, 642, 880, 894
MluCI AATT 6 cut(s) 95, 182, 582, 788, 937, 1035
MlyI GAGTC 1 cut(s) 99
MnlI CCTC 7 cut(s) 60, 323, 571, 645, 702, 1298, 1302
MseI TTAA 3 cut(s) 393, 432, 447
MslI CAYNNNNRTG 2 cut(s) 401, 964
MspI CCGG 5 cut(s) 232, 256, 265, 464, 479
MspR9I CCNGG 3 cut(s) 241, 265, 464
MvaI CCWGG 1 cut(s) 241
MvnI CGCG 4 cut(s) 213, 225, 344, 710
MwoI GCNNNNNNNGC 7 cut(s) 16, 219, 356, 365, 512, 902, 1181
NciI CCSGG 2 cut(s) 265, 464
NdeII GATC 2 cut(s) 666, 1103
NlaIII CATG 4 cut(s) 400, 545, 1059, 1141
NlaIV GGNNCC 2 cut(s) 204, 263
NmuCI GTSAC 1 cut(s) 853
NruI TCGCGA 1 cut(s) 344
NspI RCATGY 2 cut(s) 400, 1059
PfeI GAWTC 4 cut(s) 688, 834, 925, 1141
PflFI GACNNNGTC 1 cut(s) 658
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 9 cut(s) 7, 12, 160, 221, 224, 358, 514, 904, 949
PleI GAGTC 1 cut(s) 99
PmaCI CACGTG 1 cut(s) 364
PmlI CACGTG 1 cut(s) 364
PpsI GAGTC 1 cut(s) 99
Ppu21I YACGTR 1 cut(s) 364
PshBI ATTAAT 1 cut(s) 432
PsiI TTATAA 1 cut(s) 117
Psp124BI GAGCTC 2 cut(s) 59, 642
Psp6I CCWGG 1 cut(s) 239
PspCI CACGTG 1 cut(s) 364
PspEI GGTNACC 1 cut(s) 750
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 2 cut(s) 204, 263
PspPI GGNCC 2 cut(s) 261, 719
PsrI GAACNNNNNNTAC 2 cut(s) 459, 491
PstNI CAGNNNCTG 1 cut(s) 551
PsyI GACNNNGTC 1 cut(s) 658
RruI TCGCGA 1 cut(s) 344
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
RseI CAYNNNNRTG 2 cut(s) 401, 964
SacI GAGCTC 2 cut(s) 59, 642
SaqAI TTAA 3 cut(s) 393, 432, 447
SatI GCNGC 9 cut(s) 6, 11, 159, 220, 223, 357, 513, 903, 948
Sau3AI GATC 2 cut(s) 666, 1103
Sau96I GGNCC 2 cut(s) 261, 719
ScaI AGTACT 1 cut(s) 458
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 3 cut(s) 241, 265, 464
SduI GDGCHC 5 cut(s) 59, 327, 642, 880, 894
SfaNI GCATC 4 cut(s) 400, 493, 956, 1200
SinI GGWCC 1 cut(s) 261
SmiMI CAYNNNNRTG 2 cut(s) 401, 964
SmlI CTYRAG 3 cut(s) 414, 575, 881
SmoI CTYRAG 3 cut(s) 414, 575, 881
Sse9I AATT 6 cut(s) 95, 182, 582, 788, 937, 1035
SsiI CCGC 9 cut(s) 5, 23, 211, 217, 220, 223, 356, 903, 1224
SspMI CTAG 4 cut(s) 14, 815, 822, 1187
SstI GAGCTC 2 cut(s) 59, 642
StyD4I CCNGG 3 cut(s) 239, 263, 462
StyI CCWWGG 2 cut(s) 507, 942
TaaI ACNGT 1 cut(s) 1087
TaiI ACGT 3 cut(s) 366, 650, 1206
TaqII GACCGA 1 cut(s) 414
TasI AATT 6 cut(s) 95, 182, 582, 788, 937, 1035
TatI WGTACW 1 cut(s) 456
TauI GCSGC 5 cut(s) 8, 222, 225, 359, 905
TfiI GAWTC 4 cut(s) 688, 834, 925, 1141
Tru1I TTAA 3 cut(s) 393, 432, 447
Tru9I TTAA 3 cut(s) 393, 432, 447
TscAI CASTG 5 cut(s) 505, 552, 568, 1027, 1182
TseFI GTSAC 1 cut(s) 853
TseI GCWGC 4 cut(s) 10, 158, 512, 947
Tsp45I GTSAC 1 cut(s) 853
TspDTI ATGAA 8 cut(s) 122, 530, 604, 694, 758, 938, 1154, 1263
TspGWI ACGGA 1 cut(s) 319
TspRI CASTG 5 cut(s) 505, 552, 568, 1027, 1182
Tth111I GACNNNGTC 1 cut(s) 658
VpaK11BI GGWCC 1 cut(s) 261
VspI ATTAAT 1 cut(s) 432
XagI CCTNNNNNAGG 1 cut(s) 1292
XapI RAATTY 2 cut(s) 95, 182
XceI RCATGY 2 cut(s) 400, 1059
XcmI CCANNNNNNNNNTGG 1 cut(s) 1144
XspI CTAG 4 cut(s) 14, 815, 822, 1187
ZrmI AGTACT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.