AT2G42130

PAP_fibrillin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004957
Physical Location & Seq
Forward (+)
17566014 .. 17568226
2213 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G42130.5

Sequence Viewer

Length: 813 bp
ATGGCGTTGATACATGGTTCGGTTCCTGGTACGTCTGCGGTTCGATTGGTTTTTTCAACCTCAGCGTCACCGTCGAGATTTTGTCTCAATGTTCCGGTGGTGAAGCAGGGTTGGAAGAATTCGTGCCGGAGAAGGGTTTTAAGAGCAATGGTGCAGGAAACAGTTCAAGGATCTCCTTTGGTTTACGCCAGAGAAATGGAGCGCCTTTCTGCTAAGGAATCGCTACTTCTCGCTTTGAAGGATGCTGGAGGCTTTGAGGCTTTAGTTACAGGGAAAACAACTAATATGCAAAGGATCGATGTTAATGAGAGGATTACGAGTCTAGAGCGGCTTAATCCAACTCCGAGACCAACCACGTCTCCTTGTTTCGAAGGCAGGTGGAATTTTGAGTGGTTTGGATCTGGAAGTCCTGGTTTACTCGCTGCTCGAGTTATATTCGAGAGGTTTCCTTCAACATTGGCGAATTTATCAAGAATGGAGATACTGATCAAGGATGCTAACGCAAAGGCGACTGCAAATATCAAACTTTTGAACTCGATAGAAAGCAAAATCATTCTGTCATCAAAATTGACTGTTGAGGGACCACTGAGATTGAAAGAAGAATATGTTGAAGGTATGCTTGAAACTCCAACAGTCATTGAAGAAGCAGTTCCTGAACAGCTAAAAAGCGCTTTGGGACAAGCTGCCACAACGTTGCAGCAGCTTCCTGCTCTTATCAAGGATACTCTAGCCAGTGGTCTGAGAATTCCCCTTAGTAAGCTACTTTATCCATTTTTTATACATTTAACCATTTTCCGCTCAAATCTGCACTGA

Protein Analysis

270

Amino Acids

29.83

Weight (kDa)

9.67

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 2 - 183 6.1e-13 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 366
Acc36I ACCTGC 1 cut(s) 366
AccBSI CCGCTC 2 cut(s) 328, 798
AciI CCGC 3 cut(s) 38, 328, 796
AclI AACGTT 1 cut(s) 692
AclWI GGATC 3 cut(s) 178, 302, 406
AcsI RAATTY 4 cut(s) 118, 382, 463, 744
AfaI GTAC 1 cut(s) 31
AfeI AGCGCT 1 cut(s) 670
AfiI CCNNNNNNNGG 1 cut(s) 133
AgsI TTSAA 9 cut(s) 57, 167, 238, 453, 532, 595, 611, 623, 641
AjiI CACGTC 1 cut(s) 357
AjnI CCWGG 2 cut(s) 25, 409
AluBI AGCT 4 cut(s) 661, 683, 703, 760
AluI AGCT 4 cut(s) 661, 683, 703, 760
Alw26I GTCTC 3 cut(s) 89, 340, 363
AlwI GGATC 3 cut(s) 178, 302, 406
AlwNI CAGNNNCTG 1 cut(s) 653
Ama87I CYCGRG 1 cut(s) 426
Aor51HI AGCGCT 1 cut(s) 670
ApeKI GCWGC 4 cut(s) 422, 683, 697, 700
ApoI RAATTY 4 cut(s) 118, 382, 463, 744
Asp700I GAANNNNTTC 2 cut(s) 162, 648
AspLEI GCGC 2 cut(s) 204, 671
AspS9I GGNCC 1 cut(s) 581
AsuHPI GGTGA 2 cut(s) 60, 112
AsuII TTCGAA 1 cut(s) 369
AvaI CYCGRG 1 cut(s) 426
AvaII GGWCC 1 cut(s) 581
BbvCI CCTCAGC 1 cut(s) 61
BbvI GCAGC 4 cut(s) 409, 670, 709, 712
BciT130I CCWGG 2 cut(s) 27, 411
BciVI GTATCC 1 cut(s) 715
BclI TGATCA 1 cut(s) 486
BcoDI GTCTC 3 cut(s) 89, 340, 363
BfaI CTAG 2 cut(s) 323, 728
BfoI RGCGCY 2 cut(s) 205, 672
BfuAI ACCTGC 1 cut(s) 366
BfuI GTATCC 1 cut(s) 715
BisI GCNGC 5 cut(s) 329, 423, 684, 698, 701
BlsI GCNGC 5 cut(s) 330, 424, 685, 699, 702
Bme1390I CCNGG 2 cut(s) 27, 411
Bme18I GGWCC 1 cut(s) 581
BmeT110I CYCGRG 1 cut(s) 426
BmgBI CACGTC 1 cut(s) 357
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 2 cut(s) 24, 582
BmrFI CCNGG 2 cut(s) 27, 411
BmsI GCATC 2 cut(s) 232, 484
BpmI CTGGAG 1 cut(s) 267
Bpu10I CCTNAGC 2 cut(s) 61, 213
Bpu14I TTCGAA 1 cut(s) 369
Bsa29I ATCGAT 1 cut(s) 297
BsaBI GATNNNNATC 1 cut(s) 485
BsaI GGTCTC 1 cut(s) 340
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 343, 373
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse1I ACTGG 1 cut(s) 732
Bse3DI GCAATG 1 cut(s) 153
Bse8I GATNNNNATC 1 cut(s) 485
BseBI CCWGG 2 cut(s) 27, 411
BseCI ATCGAT 1 cut(s) 297
BseGI GGATG 2 cut(s) 247, 499
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 3 cut(s) 75, 578, 731
BseNI ACTGG 1 cut(s) 732
BseXI GCAGC 4 cut(s) 409, 670, 709, 712
BsgI GTGCAG 2 cut(s) 173, 791
BshVI ATCGAT 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 426
BsiSI CCGG 2 cut(s) 95, 127
BslFI GGGAC 2 cut(s) 594, 690
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 3 cut(s) 89, 340, 363
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 2 cut(s) 594, 690
Bso31I GGTCTC 1 cut(s) 340
BsoBI CYCGRG 1 cut(s) 426
Bsp119I TTCGAA 1 cut(s) 369
Bsp143I GATC 4 cut(s) 170, 294, 398, 486
BspACI CCGC 3 cut(s) 38, 328, 796
BspCNI CTCAG 3 cut(s) 74, 579, 732
BspDI ATCGAT 1 cut(s) 297
BspLI GGNNCC 2 cut(s) 24, 582
BspMI ACCTGC 1 cut(s) 366
BspPI GGATC 3 cut(s) 178, 302, 406
BspT104I TTCGAA 1 cut(s) 369
BspTNI GGTCTC 1 cut(s) 340
BsrBI CCGCTC 2 cut(s) 328, 798
BsrDI GCAATG 1 cut(s) 153
BsrI ACTGG 1 cut(s) 732
BssMI GATC 4 cut(s) 170, 294, 398, 486
Bst2UI CCWGG 2 cut(s) 27, 411
Bst4CI ACNGT 4 cut(s) 72, 163, 574, 634
BstBI TTCGAA 1 cut(s) 369
BstDEI CTNAG 5 cut(s) 61, 213, 587, 740, 752
BstF5I GGATG 2 cut(s) 247, 499
BstH2I RGCGCY 2 cut(s) 205, 672
BstHHI GCGC 2 cut(s) 204, 671
BstKTI GATC 4 cut(s) 173, 297, 401, 489
BstMAI GTCTC 3 cut(s) 89, 340, 363
BstMBI GATC 4 cut(s) 170, 294, 398, 486
BstNI CCWGG 2 cut(s) 27, 411
BstSCI CCNGG 2 cut(s) 25, 409
BstV1I GCAGC 4 cut(s) 409, 670, 709, 712
BstX2I RGATCY 2 cut(s) 170, 398
BstXI CCANNNNNNTGG 1 cut(s) 196
BstYI RGATCY 2 cut(s) 170, 398
Bsu15I ATCGAT 1 cut(s) 297
BsuI GTATCC 1 cut(s) 715
BsuTUI ATCGAT 1 cut(s) 297
BtrI CACGTC 1 cut(s) 357
BtsCI GGATG 2 cut(s) 247, 499
BtsIMutI CAGTG 3 cut(s) 584, 739, 808
BveI ACCTGC 1 cut(s) 366
CaiI CAGNNNCTG 1 cut(s) 653
CfoI GCGC 2 cut(s) 204, 671
Cfr13I GGNCC 1 cut(s) 581
ClaI ATCGAT 1 cut(s) 297
CseI GACGC 1 cut(s) 54
Csp6I GTAC 1 cut(s) 30
CspCI CAANNNNNGTGG 2 cut(s) 573, 608
CviAII CATG 1 cut(s) 14
CviJI RGCY 8 cut(s) 252, 260, 331, 661, 683, 703, 731, 760
CviKI_1 RGCY 8 cut(s) 252, 260, 331, 661, 683, 703, 731, 760
CviQI GTAC 1 cut(s) 30
DdeI CTNAG 5 cut(s) 61, 213, 587, 740, 752
DpnI GATC 4 cut(s) 172, 296, 400, 488
DpnII GATC 4 cut(s) 170, 294, 398, 486
Eco31I GGTCTC 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 581
Eco47III AGCGCT 1 cut(s) 670
Eco88I CYCGRG 1 cut(s) 426
EcoRI GAATTC 2 cut(s) 118, 744
EcoRII CCWGG 2 cut(s) 25, 409
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 1 cut(s) 17
FaiI YATR 6 cut(s) 15, 287, 434, 606, 617, 779
FalI AAGNNNNNCTT 2 cut(s) 603, 635
FaqI GGGAC 2 cut(s) 594, 690
FatI CATG 1 cut(s) 13
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 5 cut(s) 329, 423, 684, 698, 701
FokI GGATG 2 cut(s) 254, 506
Fsp4HI GCNGC 5 cut(s) 329, 423, 684, 698, 701
FspBI CTAG 2 cut(s) 323, 728
GlaI GCGC 2 cut(s) 203, 670
GluI GCNGC 5 cut(s) 329, 423, 684, 698, 701
GsuI CTGGAG 1 cut(s) 267
HaeII RGCGCY 2 cut(s) 205, 672
HapII CCGG 2 cut(s) 95, 127
HgaI GACGC 1 cut(s) 54
HhaI GCGC 2 cut(s) 204, 671
Hin1II CATG 1 cut(s) 17
Hin6I GCGC 2 cut(s) 202, 669
HinP1I GCGC 2 cut(s) 202, 669
HinfI GANTC 2 cut(s) 218, 319
HpaII CCGG 2 cut(s) 95, 127
HphI GGTGA 2 cut(s) 60, 112
Hpy166II GTNNAC 2 cut(s) 184, 416
Hpy188I TCNGA 2 cut(s) 345, 741
Hpy188III TCNNGA 6 cut(s) 75, 323, 402, 439, 471, 653
Hpy8I GTNNAC 2 cut(s) 184, 416
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 5 cut(s) 126, 232, 365, 459, 605
HpyCH4III ACNGT 4 cut(s) 72, 163, 574, 634
HpyCH4IV ACGT 3 cut(s) 32, 356, 692
HpyCH4V TGCA 5 cut(s) 154, 289, 515, 697, 808
HpyF3I CTNAG 5 cut(s) 61, 213, 587, 740, 752
HpySE526I ACGT 3 cut(s) 32, 356, 692
Hsp92II CATG 1 cut(s) 17
HspAI GCGC 2 cut(s) 202, 669
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 4 cut(s) 170, 294, 398, 486
LmnI GCTCC 1 cut(s) 199
Lsp1109I GCAGC 4 cut(s) 409, 670, 709, 712
LweI GCATC 2 cut(s) 232, 484
MaeI CTAG 2 cut(s) 323, 728
MaeII ACGT 3 cut(s) 32, 356, 692
MaeIII GTNAC 2 cut(s) 66, 265
MalI GATC 4 cut(s) 172, 296, 400, 488
MbiI CCGCTC 2 cut(s) 328, 798
MboI GATC 4 cut(s) 170, 294, 398, 486
MboII GAAGA 3 cut(s) 127, 611, 653
MflI RGATCY 2 cut(s) 170, 398
MluCI AATT 5 cut(s) 118, 382, 463, 566, 744
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 3 cut(s) 92, 362, 653
MnlI CCTC 6 cut(s) 70, 242, 250, 303, 435, 571
MroXI GAANNNNTTC 2 cut(s) 162, 648
MseI TTAA 4 cut(s) 140, 303, 333, 785
MspI CCGG 2 cut(s) 95, 127
MspR9I CCNGG 2 cut(s) 27, 411
MvaI CCWGG 2 cut(s) 27, 411
NdeII GATC 4 cut(s) 170, 294, 398, 486
NlaIII CATG 1 cut(s) 17
NlaIV GGNNCC 2 cut(s) 24, 582
NmuCI GTSAC 1 cut(s) 66
NspV TTCGAA 1 cut(s) 369
PaeR7I CTCGAG 1 cut(s) 426
PaqCI CACCTGC 1 cut(s) 366
PdmI GAANNNNTTC 2 cut(s) 162, 648
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 5 cut(s) 330, 424, 685, 699, 702
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
Psp1406I AACGTT 1 cut(s) 692
Psp6I CCWGG 2 cut(s) 25, 409
PspGI CCWGG 2 cut(s) 25, 409
PspN4I GGNNCC 2 cut(s) 24, 582
PspPI GGNCC 1 cut(s) 581
PspXI VCTCGAGB 1 cut(s) 426
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 2 cut(s) 170, 398
RsaI GTAC 1 cut(s) 31
RsaNI GTAC 1 cut(s) 30
SaqAI TTAA 4 cut(s) 140, 303, 333, 785
SatI GCNGC 5 cut(s) 329, 423, 684, 698, 701
Sau3AI GATC 4 cut(s) 170, 294, 398, 486
Sau96I GGNCC 1 cut(s) 581
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 2 cut(s) 27, 411
SfaNI GCATC 2 cut(s) 232, 484
Sfr274I CTCGAG 1 cut(s) 426
SfuI TTCGAA 1 cut(s) 369
SinI GGWCC 1 cut(s) 581
SlaI CTCGAG 1 cut(s) 426
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
Sse9I AATT 5 cut(s) 118, 382, 463, 566, 744
SsiI CCGC 3 cut(s) 38, 328, 796
SspMI CTAG 2 cut(s) 323, 728
StyD4I CCNGG 2 cut(s) 25, 409
TaaI ACNGT 4 cut(s) 72, 163, 574, 634
TaiI ACGT 3 cut(s) 35, 359, 695
TaqI TCGA 7 cut(s) 43, 74, 297, 369, 427, 438, 536
TasI AATT 5 cut(s) 118, 382, 463, 566, 744
TauI GCSGC 1 cut(s) 331
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 4 cut(s) 140, 303, 333, 785
Tru9I TTAA 4 cut(s) 140, 303, 333, 785
TscAI CASTG 2 cut(s) 591, 739
TseFI GTSAC 1 cut(s) 66
TseI GCWGC 4 cut(s) 422, 683, 697, 700
Tsp45I GTSAC 1 cut(s) 66
TspRI CASTG 2 cut(s) 591, 739
VpaK11BI GGWCC 1 cut(s) 581
XapI RAATTY 4 cut(s) 118, 382, 463, 744
XbaI TCTAGA 1 cut(s) 322
XhoI CTCGAG 1 cut(s) 426
XmnI GAANNNNTTC 2 cut(s) 162, 648
XspI CTAG 2 cut(s) 323, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.