MD12G1057400.v1.1

plastid-lipid-associated protein 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004957
Physical Location & Seq
Forward (+)
6544498 .. 6557531
13034 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1057400.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGCGTCCGTGCAGAGCCAGCTCTCTGCCGTTTCTTCGATTGCCTCGCGCTGTTCGTCTCCGTCTTCACCGCCGTCGTCGTCCTTCTCTTCGACCGCTGCCACTTCGCTGGTCCCCGTTGGCAGGTCGCCGCAGCGTCGCCGGAAATGCTCCGGAGTGGGGATGATTCGGCTCCGGCGGTCCGTGTGCCGAGCGATGGTTCAGCAGGCCGTGCCCGGCGCTTCTGCTGCGTATGCTAAGGAAATGGAGAGGCTTTCCGCCAAAGAATCGCTGCTTCTCGCTTTTAGGGATGCTGGGGGTTTTGAGGCTTTAGTCACAGGGAAGACAACAGACATGGAGCGGATTGATGTGAATGAGAGGATAACTGCTTTGGAACGAGTCAATCCAACACAACGTCCTACAACGTCGCCTTTTTTGGAAGGTCGATGGAATTTCGAGTGGTTTGGATCTGGAAGCCCAGGTCTATTTGCTGCCAGATTTTTGTTTGAGAGGTTTCCTTCAACTTTGGCTAGTTTGACAAAAATGGAGGTGGTCATCAAGGATGAGAATGCAAAGATTATGGCAAAATTGAAAGTGTTGAACTCGATAGAAGGCGAGTTTACTTTGTCAACGAAGTTAACTGTGGAGGGACCAGTTCGAATGAAAGAGGAATATGTTGAGGGGGTTCTTGAGACACCAAAAGTAATTGAAGAGGCAATACCAGACCAGCTAAAAGGTGCACTTGGTCAGGCCATAAACATAGTGCAACAACTTCCTCTGCCTATTAGGGGTGCCTTTTCCAGCGGGCTGAAAGTTCCTTTAACTGGAGCTTTTGAGAGACTCTTCATGATCTCCTATCTTGATTCGGAGATACTTATAATACGAGATGCTGCTGGAGTTCCTGAAGTTCTTACAAGGTTGGAGTCTCCGCTGGAAGAAAGCGTCATAGAGTATGAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAP_fibrillin

Protein Analysis

314

Amino Acids

34.14

Weight (kDa)

7.64

Isoelectric Point (pI)

56.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 110 - 293 1.4e-11 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 857
Acc36I ACCTGC 1 cut(s) 114
AccB1I GGYRCC 1 cut(s) 770
AccBSI CCGCTC 1 cut(s) 340
AccII CGCG 1 cut(s) 49
AccIII TCCGGA 1 cut(s) 152
AciI CCGC 8 cut(s) 71, 96, 131, 178, 258, 340, 783, 908
AclWI GGATC 1 cut(s) 454
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 1 cut(s) 903
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 5 cut(s) 123, 159, 196, 767, 803
AgsI TTSAA 4 cut(s) 501, 571, 580, 689
AjnI CCWGG 1 cut(s) 457
AluBI AGCT 4 cut(s) 22, 709, 809, 939
AluI AGCT 4 cut(s) 22, 709, 809, 939
Alw21I GWGCWC 1 cut(s) 721
Alw26I GTCTC 4 cut(s) 63, 665, 811, 909
Alw44I GTGCAC 1 cut(s) 717
AlwI GGATC 1 cut(s) 454
Aor13HI TCCGGA 1 cut(s) 152
AoxI GGCC 2 cut(s) 207, 729
ApaLI GTGCAC 1 cut(s) 717
ApeKI GCWGC 6 cut(s) 98, 133, 227, 271, 470, 869
ApoI RAATTY 1 cut(s) 430
AspLEI GCGC 2 cut(s) 51, 221
AspS9I GGNCC 3 cut(s) 112, 180, 629
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 1 cut(s) 60
AsuII TTCGAA 1 cut(s) 637
AvaII GGWCC 3 cut(s) 112, 180, 629
BaeGI GKGCMC 2 cut(s) 216, 721
BanI GGYRCC 1 cut(s) 770
BarI GAAGNNNNNNTAC 2 cut(s) 681, 713
BbsI GAAGAC 2 cut(s) 57, 329
Bbv12I GWGCWC 1 cut(s) 721
BbvI GCAGC 6 cut(s) 85, 145, 214, 258, 457, 856
BccI CCATC 2 cut(s) 190, 420
BceAI ACGGC 3 cut(s) 14, 58, 194
BciT130I CCWGG 1 cut(s) 459
BcnI CCSGG 1 cut(s) 216
BcoDI GTCTC 4 cut(s) 63, 665, 811, 909
BfaI CTAG 1 cut(s) 510
BfoI RGCGCY 1 cut(s) 222
BfuAI ACCTGC 1 cut(s) 114
BisI GCNGC 7 cut(s) 99, 131, 134, 228, 272, 471, 870
BlsI GCNGC 7 cut(s) 100, 132, 135, 229, 273, 472, 871
Bme1390I CCNGG 2 cut(s) 216, 459
Bme18I GGWCC 3 cut(s) 112, 180, 629
BmgT120I GGNCC 3 cut(s) 112, 180, 629
BmiI GGNNCC 4 cut(s) 114, 173, 630, 772
BmrFI CCNGG 2 cut(s) 216, 459
BmsI GCATC 2 cut(s) 280, 856
BpiI GAAGAC 2 cut(s) 57, 329
BpmI CTGGAG 2 cut(s) 825, 894
Bpu10I CCTNAGC 1 cut(s) 237
Bpu14I TTCGAA 1 cut(s) 637
BpuEI CTTGAG 1 cut(s) 689
BpuMI CCSGG 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 457
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 5 cut(s) 123, 159, 196, 767, 803
Bse1I ACTGG 2 cut(s) 632, 808
BseAI TCCGGA 1 cut(s) 152
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 457
BseGI GGATG 3 cut(s) 168, 295, 547
BseLI CCNNNNNNNGG 5 cut(s) 123, 159, 196, 767, 803
BseNI ACTGG 2 cut(s) 632, 808
BseSI GKGCMC 2 cut(s) 216, 721
BseXI GCAGC 6 cut(s) 85, 145, 214, 258, 457, 856
BseYI CCCAGC 1 cut(s) 293
BsgI GTGCAG 1 cut(s) 32
Bsh1236I CGCG 1 cut(s) 49
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 2 cut(s) 209, 731
BshNI GGYRCC 1 cut(s) 770
BsiEI CGRYCG 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 721
BsiSI CCGG 4 cut(s) 142, 153, 175, 216
BslFI GGGAC 2 cut(s) 98, 642
BslI CCNNNNNNNGG 5 cut(s) 123, 159, 196, 767, 803
BsmAI GTCTC 4 cut(s) 63, 665, 811, 909
BsmBI CGTCTC 1 cut(s) 63
BsmFI GGGAC 2 cut(s) 98, 642
BsmI GAATGC 1 cut(s) 553
BsnI GGCC 2 cut(s) 209, 731
Bsp119I TTCGAA 1 cut(s) 637
Bsp1286I GDGCHC 2 cut(s) 216, 721
Bsp13I TCCGGA 1 cut(s) 152
Bsp143I GATC 2 cut(s) 446, 828
BspACI CCGC 8 cut(s) 71, 96, 131, 178, 258, 340, 783, 908
BspANI GGCC 2 cut(s) 209, 731
BspEI TCCGGA 1 cut(s) 152
BspFNI CGCG 1 cut(s) 49
BspHI TCATGA 1 cut(s) 825
BspLI GGNNCC 4 cut(s) 114, 173, 630, 772
BspMI ACCTGC 1 cut(s) 114
BspPI GGATC 1 cut(s) 454
BspT104I TTCGAA 1 cut(s) 637
BspT107I GGYRCC 1 cut(s) 770
BsrBI CCGCTC 1 cut(s) 340
BsrI ACTGG 2 cut(s) 632, 808
BssECI CCNNGG 1 cut(s) 457
BssMI GATC 2 cut(s) 446, 828
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 622
Bst6I CTCTTC 3 cut(s) 94, 684, 827
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 211
BstBI TTCGAA 1 cut(s) 637
BstC8I GCNNGC 3 cut(s) 20, 207, 785
BstDEI CTNAG 1 cut(s) 237
BstF5I GGATG 3 cut(s) 168, 295, 547
BstFNI CGCG 1 cut(s) 49
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 2 cut(s) 51, 221
BstKTI GATC 2 cut(s) 449, 831
BstMAI GTCTC 4 cut(s) 63, 665, 811, 909
BstMBI GATC 2 cut(s) 446, 828
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 5 cut(s) 19, 147, 211, 227, 233
BstNI CCWGG 1 cut(s) 459
BstSCI CCNGG 2 cut(s) 214, 457
BstSLI GKGCMC 2 cut(s) 216, 721
BstUI CGCG 1 cut(s) 49
BstV1I GCAGC 6 cut(s) 85, 145, 214, 258, 457, 856
BstV2I GAAGAC 2 cut(s) 57, 329
BstX2I RGATCY 1 cut(s) 446
BstXI CCANNNNNNTGG 1 cut(s) 109
BstYI RGATCY 1 cut(s) 446
BsuRI GGCC 2 cut(s) 209, 731
BtgZI GCGATG 1 cut(s) 209
BtsCI GGATG 3 cut(s) 168, 295, 547
BveI ACCTGC 1 cut(s) 114
Cac8I GCNNGC 3 cut(s) 20, 207, 785
CciI TCATGA 1 cut(s) 825
CfoI GCGC 2 cut(s) 51, 221
Cfr13I GGNCC 3 cut(s) 112, 180, 629
CpoI CGGWCCG 1 cut(s) 180
CseI GACGC 2 cut(s) 125, 910
CspI CGGWCCG 1 cut(s) 180
CviAII CATG 2 cut(s) 334, 826
DdeI CTNAG 1 cut(s) 237
DpnI GATC 2 cut(s) 448, 830
DpnII GATC 2 cut(s) 446, 828
Eam1104I CTCTTC 3 cut(s) 94, 684, 827
EarI CTCTTC 3 cut(s) 94, 684, 827
EciI GGCGGA 1 cut(s) 247
Eco47I GGWCC 3 cut(s) 112, 180, 629
Eco57I CTGAAG 1 cut(s) 903
EcoRII CCWGG 1 cut(s) 457
Esp3I CGTCTC 1 cut(s) 63
FaeI CATG 2 cut(s) 337, 829
FalI AAGNNNNNCTT 2 cut(s) 705, 737
FaqI GGGAC 2 cut(s) 98, 642
FatI CATG 2 cut(s) 333, 825
FauI CCCGC 1 cut(s) 776
Fnu4HI GCNGC 7 cut(s) 99, 131, 134, 228, 272, 471, 870
FokI GGATG 3 cut(s) 175, 302, 554
Fsp4HI GCNGC 7 cut(s) 99, 131, 134, 228, 272, 471, 870
FspBI CTAG 1 cut(s) 510
GlaI GCGC 2 cut(s) 50, 220
GluI GCNGC 7 cut(s) 99, 131, 134, 228, 272, 471, 870
GsaI CCCAGC 1 cut(s) 297
GsuI CTGGAG 2 cut(s) 825, 894
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 2 cut(s) 209, 731
HapII CCGG 4 cut(s) 142, 153, 175, 216
HgaI GACGC 2 cut(s) 125, 910
HhaI GCGC 2 cut(s) 51, 221
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 337, 829
Hin6I GCGC 2 cut(s) 49, 219
HinP1I GCGC 2 cut(s) 49, 219
HincII GTYRAC 2 cut(s) 609, 618
HindII GTYRAC 2 cut(s) 609, 618
HinfI GANTC 6 cut(s) 166, 266, 378, 819, 842, 902
HpaI GTTAAC 1 cut(s) 618
HpaII CCGG 4 cut(s) 142, 153, 175, 216
HphI GGTGA 1 cut(s) 60
Hpy166II GTNNAC 4 cut(s) 600, 609, 618, 719
Hpy188I TCNGA 1 cut(s) 847
Hpy188III TCNNGA 6 cut(s) 153, 450, 668, 826, 839, 881
Hpy8I GTNNAC 4 cut(s) 600, 609, 618, 719
Hpy99I CGWCG 4 cut(s) 79, 82, 141, 409
HpyAV CCTTC 4 cut(s) 94, 413, 507, 584
HpyCH4III ACNGT 1 cut(s) 622
HpyCH4IV ACGT 2 cut(s) 394, 404
HpyCH4V TGCA 4 cut(s) 13, 551, 719, 745
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 147, 211, 227, 233
HpyF3I CTNAG 1 cut(s) 237
HpySE526I ACGT 2 cut(s) 394, 404
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 337, 829
HspAI GCGC 2 cut(s) 49, 219
Kpn2I TCCGGA 1 cut(s) 152
KspAI GTTAAC 1 cut(s) 618
Kzo9I GATC 2 cut(s) 446, 828
LmnI GCTCC 4 cut(s) 155, 177, 337, 806
Lsp1109I GCAGC 6 cut(s) 85, 145, 214, 258, 457, 856
LweI GCATC 2 cut(s) 280, 856
MaeI CTAG 1 cut(s) 510
MaeII ACGT 2 cut(s) 394, 404
MaeIII GTNAC 1 cut(s) 313
MalI GATC 2 cut(s) 448, 830
MbiI CCGCTC 1 cut(s) 340
MboI GATC 2 cut(s) 446, 828
MboII GAAGA 7 cut(s) 27, 57, 81, 334, 701, 814, 926
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 2 cut(s) 216, 721
MluCI AATT 3 cut(s) 430, 566, 684
MlyI GAGTC 3 cut(s) 387, 813, 911
MmeI TCCRAC 2 cut(s) 410, 879
MroI TCCGGA 1 cut(s) 152
MseI TTAA 2 cut(s) 617, 800
MspA1I CMGCKG 3 cut(s) 98, 783, 910
MspI CCGG 4 cut(s) 142, 153, 175, 216
MspR9I CCNGG 2 cut(s) 216, 459
Mva1269I GAATGC 1 cut(s) 553
MvaI CCWGG 1 cut(s) 459
MvnI CGCG 1 cut(s) 49
MwoI GCNNNNNNNGC 5 cut(s) 19, 147, 211, 227, 233
NciI CCSGG 1 cut(s) 216
NdeII GATC 2 cut(s) 446, 828
NlaIII CATG 2 cut(s) 337, 829
NlaIV GGNNCC 4 cut(s) 114, 173, 630, 772
NmeAIII GCCGAG 1 cut(s) 215
NmuCI GTSAC 1 cut(s) 313
NspV TTCGAA 1 cut(s) 637
PagI TCATGA 1 cut(s) 825
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PctI GAATGC 1 cut(s) 553
PfeI GAWTC 3 cut(s) 166, 266, 842
PkrI GCNGC 7 cut(s) 100, 132, 135, 229, 273, 472, 871
PleI GAGTC 3 cut(s) 386, 813, 910
PpsI GAGTC 3 cut(s) 386, 813, 910
PsiI TTATAA 1 cut(s) 857
Psp6I CCWGG 1 cut(s) 457
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 4 cut(s) 114, 173, 630, 772
PspPI GGNCC 3 cut(s) 112, 180, 629
PsuI RGATCY 1 cut(s) 446
Rsr2I CGGWCCG 1 cut(s) 180
RsrII CGGWCCG 1 cut(s) 180
SaqAI TTAA 2 cut(s) 617, 800
SatI GCNGC 7 cut(s) 99, 131, 134, 228, 272, 471, 870
Sau3AI GATC 2 cut(s) 446, 828
Sau96I GGNCC 3 cut(s) 112, 180, 629
SchI GAGTC 3 cut(s) 387, 813, 911
ScrFI CCNGG 2 cut(s) 216, 459
SduI GDGCHC 2 cut(s) 216, 721
SfaNI GCATC 2 cut(s) 280, 856
SfuI TTCGAA 1 cut(s) 637
SinI GGWCC 3 cut(s) 112, 180, 629
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
Sse9I AATT 3 cut(s) 430, 566, 684
SsiI CCGC 8 cut(s) 71, 96, 131, 178, 258, 340, 783, 908
SspMI CTAG 1 cut(s) 510
StyD4I CCNGG 2 cut(s) 214, 457
TaaI ACNGT 1 cut(s) 622
TaiI ACGT 2 cut(s) 397, 407
TaqI TCGA 6 cut(s) 38, 92, 424, 435, 584, 637
TasI AATT 3 cut(s) 430, 566, 684
TauI GCSGC 1 cut(s) 133
TfiI GAWTC 3 cut(s) 166, 266, 842
Tru1I TTAA 2 cut(s) 617, 800
Tru9I TTAA 2 cut(s) 617, 800
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 6 cut(s) 98, 133, 227, 271, 470, 869
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 2 cut(s) 656, 814
TspGWI ACGGA 2 cut(s) 51, 172
VneI GTGCAC 1 cut(s) 717
VpaK11BI GGWCC 3 cut(s) 112, 180, 629
XapI RAATTY 1 cut(s) 430
XspI CTAG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.