Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006621

Physical Location & Seq

Genomic Coordinates Forward (+)

18451426 .. 18452875

Gene Structure 1450 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G44750.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 798 bp

ATGTTGTCAGCCATGGATGTTATGATTCACTCTTCAAGCTTTCTCCTCCCTTGCGACGAAACTTGTGGGACGAGATACGCTCTCGTTGTTCTTAACCAGAATTTACCACGATTCACTCCTCTTCTCTGGGAACATGCAAAACTTCGTCTCTGTGCTGATGGAGGCGCTAATCGCATCTACGACGAATTACCTCTCTTCTTCCCTCACGAAGACCCTTTTGTCATTCGAAACAGGTATAAGCCCGATGTTATCAAGGGAGATATGGATTCTATACGCCGTGACGTCCTCGACTTTTATGTTTACTGGGGAACTAAGGTTATAGATGAATCTCATGATCAAGATACCACTGATCTTGATAAATGCATTTCGTATATCCGTCACTCTACTTTGAATCAGGAGAGTTCCAGAATTCTTGCCACTGGAGCACTCGGGGGAAGATTCGATCATGAAGCCGGTAATCTCAACGTCTTATATCGATATCCAGACACAAGGATAGTCCTTTTATCTGATGATTGTCTCATCCAACTCCTTCCAAAGACTCATCGACATGAAATACATATTCACTCTTCTCTTCAAGGACCTCACTGTGGACTTATACCCATTGGAACTCCATCTGCCAATACCACTACCTCAGGGCTTAAATGGGATCTCAGCAACACTGAGATGAGATTTGGTGGGTTGATAAGTACATCGAACTTGGTTAAAGAAGAGATAATCACAGTCGAATCGGATTCGGATCTTCTCTGGACTATTTCCATCAAGAAGACAGGACTTCCTGTACAAGACCATAAACCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004788 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009229 GO:0009987 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0034641 GO:0042357 GO:0042723 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0051186 GO:0051188 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

TPK_B1_binding TPK_catalytic

Protein Analysis

265

Amino Acids

29.97

Weight (kDa)

5.7

Isoelectric Point (pI)

35.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPK_catalytic	PF04263	41 - 167	7e-35	Thiamin pyrophosphokinase, catalytic domain
TPK_B1_binding	PF04265	184 - 250	1.4e-22	Thiamin pyrophosphokinase, vitamin B1 binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006621)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02880 AT1G02880 AT1G02880 AT2G44750 AT2G44750
fragaria_vesca	FvH4_2g25500 FvH4_2g25500
malus_domestica	MD02G1044000.v1.1 MD02G1044100.v1.1 MD03G1285900.v1.1 MD06G1023200.v1.1 MD06G1023300.v1.1 MD11G1034700.v1.1 MD11G1305700.v1.1
prunus_persica	Prupe.8G259100_v2.0.a1
pyrus_communis	pycom03g22660 pycom11g26750
rosa_chinensis	RchiOBHm_Chr6g0293711
rosa_laevigata	RLG00000011930
rosa_roxburghii	Rroxscaffold_7G00173970
rosa_rugosa	Rorug06G0237500
rosa_samantha	Rh6AG350300 Rh6BG357800 Rh6CG364600 Rh6DG351500
rosa_wichuraiana	Rw6G030500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	218
AatII	GACGTC	1 cut(s)	285
AclWI	GGATC	2 cut(s)	654, 744
AcsI	RAATTY	2 cut(s)	100, 408
AcyI	GRCGYC	1 cut(s)	282
AfaI	GTAC	2 cut(s)	688, 780
AfiI	CCNNNNNNNGG	1 cut(s)	587
AgsI	TTSAA	3 cut(s)	36, 391, 575
AluBI	AGCT	1 cut(s)	39
AluI	AGCT	1 cut(s)	39
Alw21I	GWGCWC	1 cut(s)	427
Alw26I	GTCTC	2 cut(s)	152, 521
AlwI	GGATC	2 cut(s)	654, 744
Ama87I	CYCGRG	1 cut(s)	428
ApoI	RAATTY	2 cut(s)	100, 408
AspLEI	GCGC	1 cut(s)	167
AspS9I	GGNCC	1 cut(s)	578
AsuII	TTCGAA	1 cut(s)	226
AvaI	CYCGRG	1 cut(s)	428
AvaII	GGWCC	1 cut(s)	578
AxyI	CCTNAGG	1 cut(s)	631
BbsI	GAAGAC	2 cut(s)	216, 770
Bbv12I	GWGCWC	1 cut(s)	427
BccI	CCATC	3 cut(s)	152, 619, 764
BceAI	ACGGC	1 cut(s)	261
BclI	TGATCA	1 cut(s)	334
BcoDI	GTCTC	2 cut(s)	152, 521
BfoI	RGCGCY	1 cut(s)	168
Bme18I	GGWCC	1 cut(s)	578
BmeT110I	CYCGRG	1 cut(s)	428
BmgT120I	GGNCC	1 cut(s)	578
BmrI	ACTGGG	1 cut(s)	313
BmsI	GCATC	1 cut(s)	183
BmuI	ACTGGG	1 cut(s)	313
BpiI	GAAGAC	2 cut(s)	216, 770
BplI	GAGNNNNNCTC	2 cut(s)	64, 96
BpmI	CTGGAG	1 cut(s)	441
Bpu14I	TTCGAA	1 cut(s)	226
Bsa29I	ATCGAT	1 cut(s)	475
BsaBI	GATNNNNATC	1 cut(s)	735
BsaHI	GRCGYC	1 cut(s)	282
BsaJI	CCNNGG	1 cut(s)	12
Bsc4I	CCNNNNNNNGG	1 cut(s)	587
Bse118I	RCCGGY	1 cut(s)	452
Bse1I	ACTGG	2 cut(s)	308, 424
Bse21I	CCTNAGG	1 cut(s)	631
Bse8I	GATNNNNATC	1 cut(s)	735
BseCI	ATCGAT	1 cut(s)	475
BseDI	CCNNGG	1 cut(s)	12
BseGI	GGATG	2 cut(s)	22, 519
BseJI	GATNNNNATC	1 cut(s)	735
BseLI	CCNNNNNNNGG	1 cut(s)	587
BseMII	CTCAG	3 cut(s)	645, 651, 664
BseNI	ACTGG	2 cut(s)	308, 424
BseRI	GAGGAG	2 cut(s)	35, 108
BshVI	ATCGAT	1 cut(s)	475
BsiHKAI	GWGCWC	1 cut(s)	427
BsiHKCI	CYCGRG	1 cut(s)	428
BsiSI	CCGG	1 cut(s)	453
BslFI	GGGAC	1 cut(s)	82
BslI	CCNNNNNNNGG	1 cut(s)	587
BsmAI	GTCTC	2 cut(s)	152, 521
BsmBI	CGTCTC	1 cut(s)	152
BsmFI	GGGAC	1 cut(s)	82
BsoBI	CYCGRG	1 cut(s)	428
Bsp119I	TTCGAA	1 cut(s)	226
Bsp1286I	GDGCHC	1 cut(s)	427
Bsp1407I	TGTACA	1 cut(s)	778
Bsp143I	GATC	5 cut(s)	334, 349, 442, 646, 736
Bsp19I	CCATGG	1 cut(s)	12
BspCNI	CTCAG	3 cut(s)	644, 652, 663
BspDI	ATCGAT	1 cut(s)	475
BspHI	TCATGA	2 cut(s)	331, 445
BspPI	GGATC	2 cut(s)	654, 744
BspT104I	TTCGAA	1 cut(s)	226
BsrFI	RCCGGY	1 cut(s)	452
BsrGI	TGTACA	1 cut(s)	778
BsrI	ACTGG	2 cut(s)	308, 424
BssAI	RCCGGY	1 cut(s)	452
BssECI	CCNNGG	1 cut(s)	12
BssMI	GATC	5 cut(s)	334, 349, 442, 646, 736
BssNI	GRCGYC	1 cut(s)	282
BssT1I	CCWWGG	1 cut(s)	12
Bst4CI	ACNGT	2 cut(s)	587, 721
Bst6I	CTCTTC	6 cut(s)	37, 126, 200, 571, 576, 702
BstACI	GRCGYC	1 cut(s)	282
BstAUI	TGTACA	1 cut(s)	778
BstBI	TTCGAA	1 cut(s)	226
BstDEI	CTNAG	5 cut(s)	312, 631, 650, 660, 795
BstDSI	CCRYGG	1 cut(s)	12
BstF5I	GGATG	2 cut(s)	22, 519
BstH2I	RGCGCY	1 cut(s)	168
BstHHI	GCGC	1 cut(s)	167
BstKTI	GATC	5 cut(s)	337, 352, 445, 649, 739
BstMAI	GTCTC	2 cut(s)	152, 521
BstMBI	GATC	5 cut(s)	334, 349, 442, 646, 736
BstMWI	GCNNNNNNNGC	2 cut(s)	171, 422
BstNSI	RCATGY	1 cut(s)	137
BstV2I	GAAGAC	2 cut(s)	216, 770
BstX2I	RGATCY	2 cut(s)	646, 736
BstYI	RGATCY	2 cut(s)	646, 736
Bsu15I	ATCGAT	1 cut(s)	475
Bsu36I	CCTNAGG	1 cut(s)	631
BsuTUI	ATCGAT	1 cut(s)	475
BtgI	CCRYGG	1 cut(s)	12
BtsCI	GGATG	2 cut(s)	22, 519
BtsIMutI	CAGTG	4 cut(s)	345, 417, 583, 657
CciI	TCATGA	2 cut(s)	331, 445
CfoI	GCGC	1 cut(s)	167
Cfr10I	RCCGGY	1 cut(s)	452
Cfr13I	GGNCC	1 cut(s)	578
ClaI	ATCGAT	1 cut(s)	475
Csp6I	GTAC	2 cut(s)	687, 779
CspCI	CAANNNNNGTGG	2 cut(s)	334, 369
CviAII	CATG	5 cut(s)	13, 134, 332, 446, 548
CviJI	RGCY	5 cut(s)	11, 39, 241, 452, 637
CviKI_1	RGCY	5 cut(s)	11, 39, 241, 452, 637
CviQI	GTAC	2 cut(s)	687, 779
DdeI	CTNAG	5 cut(s)	312, 631, 650, 660, 795
DpnI	GATC	5 cut(s)	336, 351, 444, 648, 738
DpnII	GATC	5 cut(s)	334, 349, 442, 646, 736
DrdI	GACNNNNNNGTC	1 cut(s)	218
DseDI	GACNNNNNNGTC	1 cut(s)	218
Eam1104I	CTCTTC	6 cut(s)	37, 126, 200, 571, 576, 702
EarI	CTCTTC	6 cut(s)	37, 126, 200, 571, 576, 702
Eco130I	CCWWGG	1 cut(s)	12
Eco32I	GATATC	1 cut(s)	479
Eco47I	GGWCC	1 cut(s)	578
Eco81I	CCTNAGG	1 cut(s)	631
Eco88I	CYCGRG	1 cut(s)	428
EcoO109I	RGGNCCY	1 cut(s)	578
EcoRI	GAATTC	1 cut(s)	408
EcoRV	GATATC	1 cut(s)	479
EcoT14I	CCWWGG	1 cut(s)	12
EcoT22I	ATGCAT	1 cut(s)	365
ErhI	CCWWGG	1 cut(s)	12
Esp3I	CGTCTC	1 cut(s)	152
FaeI	CATG	5 cut(s)	16, 137, 335, 449, 551
FaqI	GGGAC	1 cut(s)	82
FatI	CATG	5 cut(s)	12, 133, 331, 445, 547
FbaI	TGATCA	1 cut(s)	334
FokI	GGATG	2 cut(s)	29, 506
GlaI	GCGC	1 cut(s)	166
GsuI	CTGGAG	1 cut(s)	441
HaeII	RGCGCY	1 cut(s)	168
HapII	CCGG	1 cut(s)	453
HhaI	GCGC	1 cut(s)	167
Hin1I	GRCGYC	1 cut(s)	282
Hin1II	CATG	5 cut(s)	16, 137, 335, 449, 551
Hin6I	GCGC	1 cut(s)	165
HinP1I	GCGC	1 cut(s)	165
HindIII	AAGCTT	1 cut(s)	37
HinfI	GANTC	9 cut(s)	25, 111, 266, 326, 391, 438, 538, 725, 731
HpaII	CCGG	1 cut(s)	453
Hpy166II	GTNNAC	2 cut(s)	301, 590
Hpy188I	TCNGA	3 cut(s)	508, 730, 736
Hpy8I	GTNNAC	2 cut(s)	301, 590
Hpy99I	CGWCG	2 cut(s)	59, 185
HpyAV	CCTTC	1 cut(s)	539
HpyCH4III	ACNGT	2 cut(s)	587, 721
HpyCH4IV	ACGT	2 cut(s)	282, 465
HpyCH4V	TGCA	2 cut(s)	137, 363
HpyF10VI	GCNNNNNNNGC	2 cut(s)	171, 422
HpyF3I	CTNAG	5 cut(s)	312, 631, 650, 660, 795
HpySE526I	ACGT	2 cut(s)	282, 465
Hsp92I	GRCGYC	1 cut(s)	282
Hsp92II	CATG	5 cut(s)	16, 137, 335, 449, 551
HspAI	GCGC	1 cut(s)	165
Ksp22I	TGATCA	1 cut(s)	334
Kzo9I	GATC	5 cut(s)	334, 349, 442, 646, 736
LmnI	GCTCC	1 cut(s)	422
LweI	GCATC	1 cut(s)	183
MaeII	ACGT	2 cut(s)	282, 465
MaeIII	GTNAC	2 cut(s)	278, 377
MalI	GATC	5 cut(s)	336, 351, 444, 648, 738
MboI	GATC	5 cut(s)	334, 349, 442, 646, 736
MflI	RGATCY	2 cut(s)	646, 736
MhlI	GDGCHC	1 cut(s)	427
MluCI	AATT	3 cut(s)	100, 185, 408
MlyI	GAGTC	1 cut(s)	532
MmeI	TCCRAC	1 cut(s)	547
MnlI	CCTC	8 cut(s)	56, 129, 155, 201, 213, 296, 591, 640
Mph1103I	ATGCAT	1 cut(s)	365
MseI	TTAA	3 cut(s)	93, 639, 702
MslI	CAYNNNNRTG	2 cut(s)	546, 662
MspI	CCGG	1 cut(s)	453
MwoI	GCNNNNNNNGC	2 cut(s)	171, 422
NcoI	CCATGG	1 cut(s)	12
NdeII	GATC	5 cut(s)	334, 349, 442, 646, 736
NlaIII	CATG	5 cut(s)	16, 137, 335, 449, 551
NmuCI	GTSAC	2 cut(s)	278, 377
NsiI	ATGCAT	1 cut(s)	365
NspI	RCATGY	1 cut(s)	137
NspV	TTCGAA	1 cut(s)	226
PagI	TCATGA	2 cut(s)	331, 445
PfeI	GAWTC	8 cut(s)	25, 111, 266, 326, 391, 438, 725, 731
PleI	GAGTC	1 cut(s)	532
PpsI	GAGTC	1 cut(s)	532
PpuMI	RGGWCCY	1 cut(s)	578
Psp5II	RGGWCCY	1 cut(s)	578
PspPI	GGNCC	1 cut(s)	578
PspPPI	RGGWCCY	1 cut(s)	578
PsuI	RGATCY	2 cut(s)	646, 736
RsaI	GTAC	2 cut(s)	688, 780
RsaNI	GTAC	2 cut(s)	687, 779
RseI	CAYNNNNRTG	2 cut(s)	546, 662
SaqAI	TTAA	3 cut(s)	93, 639, 702
Sau3AI	GATC	5 cut(s)	334, 349, 442, 646, 736
Sau96I	GGNCC	1 cut(s)	578
SchI	GAGTC	1 cut(s)	532
SduI	GDGCHC	1 cut(s)	427
SetI	ASST	9 cut(s)	41, 193, 236, 285, 318, 468, 583, 632, 796
SfaNI	GCATC	1 cut(s)	183
SfuI	TTCGAA	1 cut(s)	226
SinI	GGWCC	1 cut(s)	578
SmiMI	CAYNNNNRTG	2 cut(s)	546, 662
Sse9I	AATT	3 cut(s)	100, 185, 408
StyI	CCWWGG	1 cut(s)	12
TaaI	ACNGT	2 cut(s)	587, 721
TaiI	ACGT	2 cut(s)	285, 468
TaqI	TCGA	7 cut(s)	226, 288, 441, 475, 544, 692, 723
TasI	AATT	3 cut(s)	100, 185, 408
TatI	WGTACW	2 cut(s)	686, 778
TfiI	GAWTC	8 cut(s)	25, 111, 266, 326, 391, 438, 725, 731
Tru1I	TTAA	3 cut(s)	93, 639, 702
Tru9I	TTAA	3 cut(s)	93, 639, 702
TscAI	CASTG	4 cut(s)	352, 424, 590, 664
TseFI	GTSAC	2 cut(s)	278, 377
Tsp45I	GTSAC	2 cut(s)	278, 377
TspDTI	ATGAA	3 cut(s)	339, 462, 564
TspGWI	ACGGA	1 cut(s)	365
TspRI	CASTG	4 cut(s)	352, 424, 590, 664
VpaK11BI	GGWCC	1 cut(s)	578
XapI	RAATTY	2 cut(s)	100, 408
XceI	RCATGY	1 cut(s)	137
ZraI	GACGTC	1 cut(s)	283
Zsp2I	ATGCAT	1 cut(s)	365

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.