Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0006621

Physical Location & Seq

Genomic Coordinates Forward (+)

42043639 .. 42046073

Gene Structure 2435 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1305700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 792 bp

ATGACATCTAAATCCATGGACCTCATGTCTCATTCCTCCGCCTTCCTCCTCCCTACGCTCCCGCCCAACCAGACGCCGTCTCCGACCTACGCGCTCGTCGTGCTCAACCAGAGCCTCCCGAGATTCGCTCCCCTGCTCTGGAACCACGCAGCACAACTACGTCTGTGTGCGGACGGCGGTGCCAATCGCGTTTACGACGACATGCCTCTTCTGCTACCCCACGATAACGCCGTAGATGTTCGGAAAAGGTACAAGCCAGATGTTATTAAAGGCGACATGGATTCAATCCGAAAGGATGTCTTGGAATTTTATGCTAGTATGGGAACCCAAATTATTAATGAATCTGAGGATCAGGACACGACGGATCTGCATAAGTGTATAGCATACATATGTCACTCGGCACCAAACCCAGATAAGTCTAATCTATGCATTCTGGTTGCTGGGGCACTTGGTGGACGGTTTGACCACGAGATTGGAAACATCAACGTGTTATGTCGATTCTCAAACATTCGAATAATTCTCATATCTGATGATTGCCTCGTCCATCTTCTTCCAAGAACACACCGCCATGAGATTCATATTCAGTCTTCTGTTGAAGGCCCTCATTGTGGACTCGTACCCATTGGGATGCCATCTGGAAGTACTACAACCACGGGGCTTCAATGGGATCTCGCTGAAACAGAGATGAGGTTTGGTGGTCTGATAAGTACTTCAAATCTCGTCAAGGGAGAGAAAATAACAGTGCAATCCGATGCAGATCTTCTTTGGACTATAAGCATTCAAAAGATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004788 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009229 GO:0009987 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0034641 GO:0042357 GO:0042723 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0051186 GO:0051188 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

TPK_B1_binding TPK_catalytic

Protein Analysis

264

Amino Acids

29.05

Weight (kDa)

5.84

Isoelectric Point (pI)

50.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPK_catalytic	PF04263	46 - 173	1.7e-37	Thiamin pyrophosphokinase, catalytic domain
TPK_B1_binding	PF04265	189 - 255	3.3e-21	Thiamin pyrophosphokinase, vitamin B1 binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006621)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02880 AT1G02880 AT1G02880 AT2G44750 AT2G44750
fragaria_vesca	FvH4_2g25500 FvH4_2g25500
malus_domestica	MD02G1044000.v1.1 MD02G1044100.v1.1 MD03G1285900.v1.1 MD06G1023200.v1.1 MD06G1023300.v1.1 MD11G1034700.v1.1 MD11G1305700.v1.1
prunus_persica	Prupe.8G259100_v2.0.a1
pyrus_communis	pycom03g22660 pycom11g26750
rosa_chinensis	RchiOBHm_Chr6g0293711
rosa_laevigata	RLG00000011930
rosa_roxburghii	Rroxscaffold_7G00173970
rosa_rugosa	Rorug06G0237500
rosa_samantha	Rh6AG350300 Rh6BG357800 Rh6CG364600 Rh6DG351500
rosa_wichuraiana	Rw6G030500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	179, 400
AccII	CGCG	2 cut(s)	92, 189
AciI	CCGC	5 cut(s)	39, 62, 170, 177, 565
AclWI	GGATC	3 cut(s)	357, 372, 675
AcsI	RAATTY	1 cut(s)	305
AcyI	GRCGYC	1 cut(s)	74
AdeI	CACNNNGTG	1 cut(s)	452
AfaI	GTAC	4 cut(s)	251, 618, 643, 709
AfiI	CCNNNNNNNGG	2 cut(s)	138, 608
AflIII	ACRYGT	1 cut(s)	486
AgsI	TTSAA	5 cut(s)	285, 596, 662, 714, 782
AhdI	GACNNNNNGTC	1 cut(s)	25
Alw21I	GWGCWC	1 cut(s)	105
Alw26I	GTCTC	2 cut(s)	33, 84
AlwI	GGATC	3 cut(s)	357, 372, 675
Ama87I	CYCGRG	1 cut(s)	118
AoxI	GGCC	1 cut(s)	598
ApeKI	GCWGC	1 cut(s)	149
ApoI	RAATTY	1 cut(s)	305
AseI	ATTAAT	1 cut(s)	336
AspLEI	GCGC	1 cut(s)	94
AspS9I	GGNCC	2 cut(s)	19, 599
AsuII	TTCGAA	1 cut(s)	511
AvaI	CYCGRG	1 cut(s)	118
AvaII	GGWCC	1 cut(s)	19
BaeGI	GKGCMC	1 cut(s)	448
BanI	GGYRCC	2 cut(s)	179, 400
BauI	CACGAG	1 cut(s)	467
BbsI	GAAGAC	1 cut(s)	579
Bbv12I	GWGCWC	1 cut(s)	105
BbvI	GCAGC	1 cut(s)	161
BccI	CCATC	2 cut(s)	552, 640
BceAI	ACGGC	3 cut(s)	61, 190, 215
BcgI	CGANNNNNNTGC	2 cut(s)	349, 383
BcoDI	GTCTC	2 cut(s)	33, 84
BfaI	CTAG	1 cut(s)	315
BglII	AGATCT	1 cut(s)	757
BisI	GCNGC	1 cut(s)	150
BlsI	GCNGC	1 cut(s)	151
BmcAI	AGTACT	2 cut(s)	643, 709
Bme18I	GGWCC	1 cut(s)	19
BmeRI	GACNNNNNGTC	1 cut(s)	25
BmeT110I	CYCGRG	1 cut(s)	118
BmgT120I	GGNCC	2 cut(s)	19, 599
BmiI	GGNNCC	4 cut(s)	143, 181, 325, 402
BmsI	GCATC	2 cut(s)	618, 742
BpiI	GAAGAC	1 cut(s)	579
BplI	GAGNNNNNCTC	2 cut(s)	112, 144
Bpu14I	TTCGAA	1 cut(s)	511
BsaBI	GATNNNNATC	1 cut(s)	756
BsaHI	GRCGYC	1 cut(s)	74
BsaJI	CCNNGG	2 cut(s)	15, 651
BsaXI	ACNNNNNCTCC	2 cut(s)	64, 94
Bsc4I	CCNNNNNNNGG	2 cut(s)	138, 608
Bse8I	GATNNNNATC	1 cut(s)	756
BseDI	CCNNGG	2 cut(s)	15, 651
BseGI	GGATG	2 cut(s)	301, 633
BseJI	GATNNNNATC	1 cut(s)	756
BseLI	CCNNNNNNNGG	2 cut(s)	138, 608
BseMII	CTCAG	1 cut(s)	336
BseRI	GAGGAG	1 cut(s)	38
BseSI	GKGCMC	1 cut(s)	448
BseXI	GCAGC	1 cut(s)	161
BseYI	CCCAGC	1 cut(s)	440
Bsh1236I	CGCG	2 cut(s)	92, 189
BshFI	GGCC	1 cut(s)	600
BshNI	GGYRCC	2 cut(s)	179, 400
BsiHKAI	GWGCWC	1 cut(s)	105
BsiHKCI	CYCGRG	1 cut(s)	118
BslI	CCNNNNNNNGG	2 cut(s)	138, 608
BsmAI	GTCTC	2 cut(s)	33, 84
BsmBI	CGTCTC	1 cut(s)	84
BsmI	GAATGC	2 cut(s)	429, 777
BsnI	GGCC	1 cut(s)	600
BsoBI	CYCGRG	1 cut(s)	118
Bsp119I	TTCGAA	1 cut(s)	511
Bsp1286I	GDGCHC	2 cut(s)	105, 448
Bsp143I	GATC	4 cut(s)	349, 364, 667, 757
Bsp19I	CCATGG	1 cut(s)	15
BspACI	CCGC	5 cut(s)	39, 62, 170, 177, 565
BspANI	GGCC	1 cut(s)	600
BspCNI	CTCAG	1 cut(s)	337
BspFNI	CGCG	2 cut(s)	92, 189
BspLI	GGNNCC	4 cut(s)	143, 181, 325, 402
BspPI	GGATC	3 cut(s)	357, 372, 675
BspT104I	TTCGAA	1 cut(s)	511
BspT107I	GGYRCC	2 cut(s)	179, 400
BssECI	CCNNGG	2 cut(s)	15, 651
BssMI	GATC	4 cut(s)	349, 364, 667, 757
BssNI	GRCGYC	1 cut(s)	74
BssSI	CACGAG	1 cut(s)	467
BssT1I	CCWWGG	1 cut(s)	15
Bst2BI	CACGAG	1 cut(s)	467
Bst4CI	ACNGT	2 cut(s)	459, 742
Bst6I	CTCTTC	1 cut(s)	213
BstACI	GRCGYC	1 cut(s)	74
BstBI	TTCGAA	1 cut(s)	511
BstDEI	CTNAG	1 cut(s)	345
BstDSI	CCRYGG	2 cut(s)	15, 651
BstF5I	GGATG	2 cut(s)	301, 633
BstFNI	CGCG	2 cut(s)	92, 189
BstHHI	GCGC	1 cut(s)	94
BstKTI	GATC	4 cut(s)	352, 367, 670, 760
BstMAI	GTCTC	2 cut(s)	33, 84
BstMBI	GATC	4 cut(s)	349, 364, 667, 757
BstMWI	GCNNNNNNNGC	2 cut(s)	100, 211
BstNSI	RCATGY	1 cut(s)	205
BstSLI	GKGCMC	1 cut(s)	448
BstUI	CGCG	2 cut(s)	92, 189
BstV1I	GCAGC	1 cut(s)	161
BstV2I	GAAGAC	1 cut(s)	579
BstX2I	RGATCY	3 cut(s)	364, 667, 757
BstXI	CCANNNNNNTGG	1 cut(s)	473
BstYI	RGATCY	3 cut(s)	364, 667, 757
BsuRI	GGCC	1 cut(s)	600
BtgI	CCRYGG	2 cut(s)	15, 651
BtsCI	GGATG	2 cut(s)	301, 633
BtsIMutI	CAGTG	1 cut(s)	747
CfoI	GCGC	1 cut(s)	94
Cfr13I	GGNCC	2 cut(s)	19, 599
CseI	GACGC	1 cut(s)	82
Csp6I	GTAC	4 cut(s)	250, 617, 642, 708
CviAII	CATG	5 cut(s)	16, 25, 202, 277, 569
CviJI	RGCY	4 cut(s)	114, 256, 600, 658
CviKI_1	RGCY	4 cut(s)	114, 256, 600, 658
CviQI	GTAC	4 cut(s)	250, 617, 642, 708
DdeI	CTNAG	1 cut(s)	345
DpnI	GATC	4 cut(s)	351, 366, 669, 759
DpnII	GATC	4 cut(s)	349, 364, 667, 757
DraIII	CACNNNGTG	1 cut(s)	452
DriI	GACNNNNNGTC	1 cut(s)	25
Eam1104I	CTCTTC	1 cut(s)	213
Eam1105I	GACNNNNNGTC	1 cut(s)	25
EarI	CTCTTC	1 cut(s)	213
EciI	GGCGGA	1 cut(s)	28
Eco130I	CCWWGG	1 cut(s)	15
Eco47I	GGWCC	1 cut(s)	19
Eco88I	CYCGRG	1 cut(s)	118
EcoO109I	RGGNCCY	1 cut(s)	599
EcoT14I	CCWWGG	1 cut(s)	15
EcoT22I	ATGCAT	1 cut(s)	431
ErhI	CCWWGG	1 cut(s)	15
Esp3I	CGTCTC	1 cut(s)	84
FaeI	CATG	5 cut(s)	19, 28, 205, 280, 572
FalI	AAGNNNNNCTT	2 cut(s)	284, 316
FatI	CATG	5 cut(s)	15, 24, 201, 276, 568
FauI	CCCGC	1 cut(s)	69
FauNDI	CATATG	1 cut(s)	389
Fnu4HI	GCNGC	1 cut(s)	150
FokI	GGATG	2 cut(s)	308, 640
Fsp4HI	GCNGC	1 cut(s)	150
FspBI	CTAG	1 cut(s)	315
GlaI	GCGC	1 cut(s)	93
GluI	GCNGC	1 cut(s)	150
GsaI	CCCAGC	1 cut(s)	444
HaeIII	GGCC	1 cut(s)	600
HgaI	GACGC	1 cut(s)	82
HhaI	GCGC	1 cut(s)	94
Hin1I	GRCGYC	1 cut(s)	74
Hin1II	CATG	5 cut(s)	19, 28, 205, 280, 572
Hin6I	GCGC	1 cut(s)	92
HinP1I	GCGC	1 cut(s)	92
HinfI	GANTC	6 cut(s)	123, 281, 341, 498, 574, 612
Hpy166II	GTNNAC	3 cut(s)	193, 455, 611
Hpy188I	TCNGA	7 cut(s)	84, 243, 290, 346, 529, 702, 751
Hpy188III	TCNNGA	4 cut(s)	118, 139, 353, 636
Hpy8I	GTNNAC	3 cut(s)	193, 455, 611
Hpy99I	CGWCG	3 cut(s)	101, 200, 364
HpyAV	CCTTC	2 cut(s)	52, 590
HpyCH4III	ACNGT	2 cut(s)	459, 742
HpyCH4IV	ACGT	2 cut(s)	160, 486
HpyCH4V	TGCA	4 cut(s)	370, 429, 745, 755
HpyF10VI	GCNNNNNNNGC	2 cut(s)	100, 211
HpyF3I	CTNAG	1 cut(s)	345
HpySE526I	ACGT	2 cut(s)	160, 486
Hsp92I	GRCGYC	1 cut(s)	74
Hsp92II	CATG	5 cut(s)	19, 28, 205, 280, 572
HspAI	GCGC	1 cut(s)	92
Kzo9I	GATC	4 cut(s)	349, 364, 667, 757
LmnI	GCTCC	2 cut(s)	63, 133
Lsp1109I	GCAGC	1 cut(s)	161
LweI	GCATC	2 cut(s)	618, 742
MaeI	CTAG	1 cut(s)	315
MaeII	ACGT	2 cut(s)	160, 486
MaeIII	GTNAC	1 cut(s)	392
MalI	GATC	4 cut(s)	351, 366, 669, 759
MboI	GATC	4 cut(s)	349, 364, 667, 757
MboII	GAAGA	5 cut(s)	200, 539, 542, 579, 752
MflI	RGATCY	3 cut(s)	364, 667, 757
MhlI	GDGCHC	2 cut(s)	105, 448
MluCI	AATT	3 cut(s)	305, 330, 516
MlyI	GAGTC	1 cut(s)	606
MmeI	TCCRAC	1 cut(s)	107
Mph1103I	ATGCAT	1 cut(s)	431
MseI	TTAA	2 cut(s)	267, 336
MslI	CAYNNNNRTG	4 cut(s)	388, 485, 567, 626
Mva1269I	GAATGC	2 cut(s)	429, 777
MvnI	CGCG	2 cut(s)	92, 189
MwoI	GCNNNNNNNGC	2 cut(s)	100, 211
NcoI	CCATGG	1 cut(s)	15
NdeI	CATATG	1 cut(s)	389
NdeII	GATC	4 cut(s)	349, 364, 667, 757
NlaIII	CATG	5 cut(s)	19, 28, 205, 280, 572
NlaIV	GGNNCC	4 cut(s)	143, 181, 325, 402
NmeAIII	GCCGAG	1 cut(s)	377
NmuCI	GTSAC	1 cut(s)	392
NsiI	ATGCAT	1 cut(s)	431
NspI	RCATGY	1 cut(s)	205
NspV	TTCGAA	1 cut(s)	511
PcsI	WCGNNNNNNNCGW	3 cut(s)	80, 96, 228
PctI	GAATGC	2 cut(s)	429, 777
PfeI	GAWTC	5 cut(s)	123, 281, 341, 498, 574
PflFI	GACNNNGTC	1 cut(s)	76
PkrI	GCNGC	1 cut(s)	151
PleI	GAGTC	1 cut(s)	606
PpsI	GAGTC	1 cut(s)	606
PshBI	ATTAAT	1 cut(s)	336
PspFI	CCCAGC	1 cut(s)	440
PspN4I	GGNNCC	4 cut(s)	143, 181, 325, 402
PspPI	GGNCC	2 cut(s)	19, 599
PsuI	RGATCY	3 cut(s)	364, 667, 757
PsyI	GACNNNGTC	1 cut(s)	76
RsaI	GTAC	4 cut(s)	251, 618, 643, 709
RsaNI	GTAC	4 cut(s)	250, 617, 642, 708
RseI	CAYNNNNRTG	4 cut(s)	388, 485, 567, 626
SaqAI	TTAA	2 cut(s)	267, 336
SatI	GCNGC	1 cut(s)	150
Sau3AI	GATC	4 cut(s)	349, 364, 667, 757
Sau96I	GGNCC	2 cut(s)	19, 599
ScaI	AGTACT	2 cut(s)	643, 709
SchI	GAGTC	1 cut(s)	606
SduI	GDGCHC	2 cut(s)	105, 448
SetI	ASST	6 cut(s)	24, 89, 163, 251, 489, 692
SfaNI	GCATC	2 cut(s)	618, 742
SfuI	TTCGAA	1 cut(s)	511
SinI	GGWCC	1 cut(s)	19
SmiMI	CAYNNNNRTG	4 cut(s)	388, 485, 567, 626
Sse9I	AATT	3 cut(s)	305, 330, 516
SsiI	CCGC	5 cut(s)	39, 62, 170, 177, 565
SspMI	CTAG	1 cut(s)	315
StyI	CCWWGG	1 cut(s)	15
TaaI	ACNGT	2 cut(s)	459, 742
TaiI	ACGT	2 cut(s)	163, 489
TaqI	TCGA	2 cut(s)	496, 511
TasI	AATT	3 cut(s)	305, 330, 516
TatI	WGTACW	2 cut(s)	641, 707
TfiI	GAWTC	5 cut(s)	123, 281, 341, 498, 574
Tru1I	TTAA	2 cut(s)	267, 336
Tru9I	TTAA	2 cut(s)	267, 336
TscAI	CASTG	1 cut(s)	747
TseFI	GTSAC	1 cut(s)	392
TseI	GCWGC	1 cut(s)	149
Tsp45I	GTSAC	1 cut(s)	392
TspDTI	ATGAA	2 cut(s)	354, 566
TspGWI	ACGGA	1 cut(s)	377
TspRI	CASTG	1 cut(s)	747
Tth111I	GACNNNGTC	1 cut(s)	76
VpaK11BI	GGWCC	1 cut(s)	19
VspI	ATTAAT	1 cut(s)	336
XapI	RAATTY	1 cut(s)	305
XceI	RCATGY	1 cut(s)	205
XspI	CTAG	1 cut(s)	315
ZrmI	AGTACT	2 cut(s)	643, 709
Zsp2I	ATGCAT	1 cut(s)	431

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.