MD11G1034700.v1.1

Belongs to the thiamine pyrophosphokinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006621
Physical Location & Seq
Forward (+)
3063740 .. 3064928
1189 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1034700.v1.1.491

Sequence Viewer

Length: 504 bp
ATGCAGGGAACTCAAATTATTAATGAATCTGAGGATCAGGACACGACCGATTTGCATAAGTGTATAGCATACATATGTGACTCGGCACCAAACCTAGATAAGTCTAATGTATGTTGTGTATTTATTATCGTTCCAACATTCGTTTTGTTTTTCTCTACTGGAATTGAGTCTGATTTTGTAGTTATGTCGATTGGAAACATCAATGTGTTATGTCGATTCTCAAACATTCAAATAATTCTCATATCTGATGATTGCTTCATCCATCTTCACACCGCCATGAGATCCATATTCAGTCTTCTGTTGAAGGCCCTCATTGTGGACTCATACCCATTGGGATGCCATCTGGAAGTACTACAACCACTGGACTTCAATGGGATCTCGGTGACTGAAACGGAGATGAGGTTTGGTGGTCTGATAAGTACATCGAATATCGTCAAGGGAGAGAAAATAACAGTGCAGTCAGATTCAGATCTTCTATGGACTATAAGCATTAAAAAGATGTAG

Protein Analysis

168

Amino Acids

18.59

Weight (kDa)

4.65

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPK_B1_binding PF04265 128 - 159 3.1e-06 Thiamin pyrophosphokinase, vitamin B1 binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 85
AciI CCGC 1 cut(s) 273
AclWI GGATC 3 cut(s) 42, 276, 383
AfaI GTAC 2 cut(s) 351, 421
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 3 cut(s) 230, 304, 370
AlwI GGATC 3 cut(s) 42, 276, 383
AoxI GGCC 1 cut(s) 306
AseI ATTAAT 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 307
AsuHPI GGTGA 1 cut(s) 394
BanI GGYRCC 1 cut(s) 85
BbsI GAAGAC 1 cut(s) 287
BccI CCATC 2 cut(s) 270, 348
BcgI CGANNNNNNTGC 2 cut(s) 34, 68
BfaI CTAG 1 cut(s) 95
BglII AGATCT 1 cut(s) 469
BmcAI AGTACT 1 cut(s) 351
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 1 cut(s) 87
BmsI GCATC 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 287
BsaBI GATNNNNATC 1 cut(s) 468
BsaXI ACNNNNNCTCC 2 cut(s) 386, 416
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 2 cut(s) 163, 366
Bse8I GATNNNNATC 1 cut(s) 468
BseGI GGATG 2 cut(s) 258, 341
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMII CTCAG 1 cut(s) 21
BseNI ACTGG 2 cut(s) 163, 366
BsgI GTGCAG 1 cut(s) 476
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 1 cut(s) 308
BshNI GGYRCC 1 cut(s) 85
BsiEI CGRYCG 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 316
BsnI GGCC 1 cut(s) 308
Bsp143I GATC 4 cut(s) 34, 281, 375, 469
BspACI CCGC 1 cut(s) 273
BspANI GGCC 1 cut(s) 308
BspCNI CTCAG 1 cut(s) 22
BspLI GGNNCC 1 cut(s) 87
BspPI GGATC 3 cut(s) 42, 276, 383
BspT107I GGYRCC 1 cut(s) 85
BsrI ACTGG 2 cut(s) 163, 366
BssMI GATC 4 cut(s) 34, 281, 375, 469
Bst4CI ACNGT 1 cut(s) 454
BstDEI CTNAG 1 cut(s) 30
BstF5I GGATG 2 cut(s) 258, 341
BstKTI GATC 4 cut(s) 37, 284, 378, 472
BstMBI GATC 4 cut(s) 34, 281, 375, 469
BstMCI CGRYCG 1 cut(s) 48
BstV2I GAAGAC 1 cut(s) 287
BstX2I RGATCY 3 cut(s) 281, 375, 469
BstYI RGATCY 3 cut(s) 281, 375, 469
BsuRI GGCC 1 cut(s) 308
BtsCI GGATG 2 cut(s) 258, 341
BtsIMutI CAGTG 2 cut(s) 359, 459
Cfr13I GGNCC 1 cut(s) 307
Csp6I GTAC 2 cut(s) 350, 420
CviAII CATG 1 cut(s) 277
CviJI RGCY 1 cut(s) 308
CviKI_1 RGCY 1 cut(s) 308
CviQI GTAC 2 cut(s) 350, 420
DdeI CTNAG 1 cut(s) 30
DpnI GATC 4 cut(s) 36, 283, 377, 471
DpnII GATC 4 cut(s) 34, 281, 375, 469
EcoO109I RGGNCCY 1 cut(s) 307
FaeI CATG 1 cut(s) 280
FatI CATG 1 cut(s) 276
FauNDI CATATG 1 cut(s) 74
FokI GGATG 2 cut(s) 245, 348
FspBI CTAG 1 cut(s) 95
HaeIII GGCC 1 cut(s) 308
Hin1II CATG 1 cut(s) 280
HinfI GANTC 6 cut(s) 26, 80, 167, 216, 320, 464
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 6 cut(s) 31, 172, 247, 414, 463, 469
Hpy188III TCNNGA 2 cut(s) 38, 344
Hpy8I GTNNAC 1 cut(s) 319
HpyAV CCTTC 1 cut(s) 298
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4V TGCA 3 cut(s) 4, 55, 457
HpyF3I CTNAG 1 cut(s) 30
Hsp92II CATG 1 cut(s) 280
Kzo9I GATC 4 cut(s) 34, 281, 375, 469
LpnPI CCDG 4 cut(s) 23, 144, 329, 347
LweI GCATC 1 cut(s) 326
MaeI CTAG 1 cut(s) 95
MaeIII GTNAC 2 cut(s) 77, 382
MalI GATC 4 cut(s) 36, 283, 377, 471
MboI GATC 4 cut(s) 34, 281, 375, 469
MboII GAAGA 3 cut(s) 257, 287, 464
MflI RGATCY 3 cut(s) 281, 375, 469
MluCI AATT 3 cut(s) 15, 162, 234
MlyI GAGTC 3 cut(s) 74, 176, 314
MmeI TCCRAC 1 cut(s) 158
MnlI CCTC 3 cut(s) 25, 320, 393
MseI TTAA 2 cut(s) 21, 492
MslI CAYNNNNRTG 4 cut(s) 73, 203, 275, 334
NdeI CATATG 1 cut(s) 74
NdeII GATC 4 cut(s) 34, 281, 375, 469
NlaIII CATG 1 cut(s) 280
NlaIV GGNNCC 1 cut(s) 87
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 2 cut(s) 77, 382
PfeI GAWTC 3 cut(s) 26, 216, 464
PleI GAGTC 3 cut(s) 74, 175, 314
PpsI GAGTC 3 cut(s) 74, 175, 314
PshBI ATTAAT 1 cut(s) 21
PspN4I GGNNCC 1 cut(s) 87
PspPI GGNCC 1 cut(s) 307
PsuI RGATCY 3 cut(s) 281, 375, 469
RsaI GTAC 2 cut(s) 351, 421
RsaNI GTAC 2 cut(s) 350, 420
RseI CAYNNNNRTG 4 cut(s) 73, 203, 275, 334
SaqAI TTAA 2 cut(s) 21, 492
Sau3AI GATC 4 cut(s) 34, 281, 375, 469
Sau96I GGNCC 1 cut(s) 307
ScaI AGTACT 1 cut(s) 351
SchI GAGTC 3 cut(s) 74, 176, 314
SetI ASST 2 cut(s) 96, 404
SfaNI GCATC 1 cut(s) 326
SmiMI CAYNNNNRTG 4 cut(s) 73, 203, 275, 334
Sse9I AATT 3 cut(s) 15, 162, 234
SsiI CCGC 1 cut(s) 273
SspMI CTAG 1 cut(s) 95
TaaI ACNGT 1 cut(s) 454
TaqI TCGA 3 cut(s) 188, 214, 425
TaqII GACCGA 1 cut(s) 62
TasI AATT 3 cut(s) 15, 162, 234
TatI WGTACW 2 cut(s) 349, 419
TfiI GAWTC 3 cut(s) 26, 216, 464
Tru1I TTAA 2 cut(s) 21, 492
Tru9I TTAA 2 cut(s) 21, 492
TscAI CASTG 2 cut(s) 366, 459
TseFI GTSAC 2 cut(s) 77, 382
Tsp45I GTSAC 2 cut(s) 77, 382
TspDTI ATGAA 2 cut(s) 39, 247
TspGWI ACGGA 1 cut(s) 407
TspRI CASTG 2 cut(s) 366, 459
VspI ATTAAT 1 cut(s) 21
XspI CTAG 1 cut(s) 95
ZrmI AGTACT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.