Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0006621

Physical Location & Seq

Genomic Coordinates Forward (+)

20664898 .. 20667022

Gene Structure 2125 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g25500.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 777 bp

ATGGAGCTCATGTCTCATTCCTCGACATTTCTCCTCCCGCCGCCGTCGTCCAATCAAACGACGCCGTCGCTGACGTATGCGCTCGTGGTGCTTAACCAGCGCCTCCCGAGGTTCTCTCCATTGCTGTGGAGCCACGCACAGCTGCGATTGTGTGCCGACGGCGGTGCGAATCGGCTTTATGACGACATGCCTCTGCTCTTGCCTCATGAAGACGCATCGGTTGTTCGCCAAAGGTACAAGCCTGACGTTATTAAAGGTGACTTGGATTCAATCAGAAAGGAAGTCTTGGAGTTTTATGCTGATATGGGAACCAAGGTAGTTGATGAGTCTCATGATCAGGACACAACCGATCTTCACAAGTGTGTGGCGTACGTATGTGACTGGGCACAGAACCTGGACAAGTCTAATCTGTGCATTCTGGTTGCTGGAGCACTCGGTGGAAGGTTTGATCATGAGATAGGAAACATCAACGTGCTGTGTCGTTTCTCGTCCGTTCGAATGATCCTTCTTTCTGACGATTGCCTCATCCACCTTCTTCCACGCACACACTGTCATGAGATACATATTCAATCTTCAGCTGAAGGTCCTCATTGTGGACTTGTCCCCATCGGGACTCCTTCTGGAAGTACAACAACTACCGGGCTTCAATGGGATCTAAATGAAACCGAGATGAGGTTTGGTGGTTTGATAAGTACTTCAAACATTGTCAAAGAAGACAAAATAACAGTGCAATCTGATTCAGATCTTCTCTGGACTATAACCATAAAAAAGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004788 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009229 GO:0009987 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0034641 GO:0042357 GO:0042723 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0051186 GO:0051188 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

TPK_B1_binding TPK_catalytic

Protein Analysis

259

Amino Acids

28.72

Weight (kDa)

5.48

Isoelectric Point (pI)

47.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPK_catalytic	PF04263	41 - 164	5.2e-38	Thiamin pyrophosphokinase, catalytic domain
TPK_B1_binding	PF04265	185 - 250	1.3e-22	Thiamin pyrophosphokinase, vitamin B1 binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006621)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02880 AT1G02880 AT1G02880 AT2G44750 AT2G44750
fragaria_vesca	FvH4_2g25500 FvH4_2g25500
malus_domestica	MD02G1044000.v1.1 MD02G1044100.v1.1 MD03G1285900.v1.1 MD06G1023200.v1.1 MD06G1023300.v1.1 MD11G1034700.v1.1 MD11G1305700.v1.1
prunus_persica	Prupe.8G259100_v2.0.a1
pyrus_communis	pycom03g22660 pycom11g26750
rosa_chinensis	RchiOBHm_Chr6g0293711
rosa_laevigata	RLG00000011930
rosa_roxburghii	Rroxscaffold_7G00173970
rosa_rugosa	Rorug06G0237500
rosa_samantha	Rh6AG350300 Rh6BG357800 Rh6CG364600 Rh6DG351500
rosa_wichuraiana	Rw6G030500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	38, 41, 162
AclWI	GGATC	2 cut(s)	496, 660
AcuI	CTGAAG	2 cut(s)	558, 600
AcyI	GRCGYC	1 cut(s)	62
AdeI	CACNNNGTG	1 cut(s)	437
AfaI	GTAC	4 cut(s)	236, 371, 628, 694
AfiI	CCNNNNNNNGG	2 cut(s)	593, 672
AgsI	TTSAA	4 cut(s)	270, 569, 647, 699
AjnI	CCWGG	1 cut(s)	393
AleI	CACNNNNGTG	1 cut(s)	360
AluBI	AGCT	4 cut(s)	7, 142, 578, 772
AluI	AGCT	4 cut(s)	7, 142, 578, 772
Alw21I	GWGCWC	2 cut(s)	9, 433
Alw26I	GTCTC	2 cut(s)	18, 333
AlwI	GGATC	2 cut(s)	496, 660
AlwNI	CAGNNNCTG	1 cut(s)	394
Ama87I	CYCGRG	1 cut(s)	106
ApeKI	GCWGC	1 cut(s)	142
AspLEI	GCGC	2 cut(s)	82, 102
AspS9I	GGNCC	1 cut(s)	584
AsuC2I	CCSGG	1 cut(s)	640
AsuHPI	GGTGA	1 cut(s)	269
AsuII	TTCGAA	1 cut(s)	496
AvaI	CYCGRG	1 cut(s)	106
AvaII	GGWCC	1 cut(s)	584
BaeGI	GKGCMC	1 cut(s)	388
BanII	GRGCYC	1 cut(s)	9
BauI	CACGAG	1 cut(s)	83
BbsI	GAAGAC	2 cut(s)	216, 720
Bbv12I	GWGCWC	2 cut(s)	9, 433
BbvI	GCAGC	1 cut(s)	129
BccI	CCATC	1 cut(s)	614
BceAI	ACGGC	3 cut(s)	28, 49, 175
BcgI	CGANNNNNNTGC	2 cut(s)	146, 180
BciT130I	CCWGG	1 cut(s)	395
BclI	TGATCA	2 cut(s)	334, 448
BcnI	CCSGG	1 cut(s)	640
BcoDI	GTCTC	2 cut(s)	18, 333
BfmI	CTRYAG	1 cut(s)	773
BfoI	RGCGCY	1 cut(s)	103
BglII	AGATCT	1 cut(s)	742
BisI	GCNGC	2 cut(s)	41, 143
BlsI	GCNGC	2 cut(s)	42, 144
BmcAI	AGTACT	1 cut(s)	694
Bme1390I	CCNGG	2 cut(s)	395, 640
Bme18I	GGWCC	1 cut(s)	584
BmeT110I	CYCGRG	1 cut(s)	106
BmgT120I	GGNCC	1 cut(s)	584
BmiI	GGNNCC	2 cut(s)	131, 310
BmrFI	CCNGG	2 cut(s)	395, 640
BmrI	ACTGGG	1 cut(s)	391
BmsI	GCATC	1 cut(s)	224
BmuI	ACTGGG	1 cut(s)	391
BpiI	GAAGAC	2 cut(s)	216, 720
BplI	GAGNNNNNCTC	2 cut(s)	100, 132
BpmI	CTGGAG	1 cut(s)	447
Bpu14I	TTCGAA	1 cut(s)	496
BpuMI	CCSGG	1 cut(s)	640
BsaAI	YACGTR	1 cut(s)	373
BsaBI	GATNNNNATC	1 cut(s)	741
BsaHI	GRCGYC	1 cut(s)	62
BsaJI	CCNNGG	2 cut(s)	107, 312
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	2 cut(s)	593, 672
Bse1I	ACTGG	1 cut(s)	386
Bse3DI	GCAATG	1 cut(s)	119
Bse8I	GATNNNNATC	1 cut(s)	741
BseBI	CCWGG	1 cut(s)	395
BseDI	CCNNGG	2 cut(s)	107, 312
BseGI	GGATG	1 cut(s)	525
BseJI	GATNNNNATC	1 cut(s)	741
BseLI	CCNNNNNNNGG	2 cut(s)	593, 672
BseMI	GCAATG	1 cut(s)	119
BseNI	ACTGG	1 cut(s)	386
BseRI	GAGGAG	1 cut(s)	23
BseSI	GKGCMC	1 cut(s)	388
BseXI	GCAGC	1 cut(s)	129
BsiHKAI	GWGCWC	2 cut(s)	9, 433
BsiHKCI	CYCGRG	1 cut(s)	106
BsiSI	CCGG	1 cut(s)	639
BsiWI	CGTACG	1 cut(s)	369
BslFI	GGGAC	2 cut(s)	587, 625
BslI	CCNNNNNNNGG	2 cut(s)	593, 672
BsmAI	GTCTC	2 cut(s)	18, 333
BsmFI	GGGAC	2 cut(s)	587, 625
BsmI	GAATGC	1 cut(s)	414
BsoBI	CYCGRG	1 cut(s)	106
Bsp119I	TTCGAA	1 cut(s)	496
Bsp1286I	GDGCHC	3 cut(s)	9, 388, 433
Bsp143I	GATC	6 cut(s)	334, 349, 448, 501, 652, 742
BspACI	CCGC	3 cut(s)	38, 41, 162
BspHI	TCATGA	4 cut(s)	205, 331, 451, 553
BspLI	GGNNCC	2 cut(s)	131, 310
BspPI	GGATC	2 cut(s)	496, 660
BspT104I	TTCGAA	1 cut(s)	496
BsrDI	GCAATG	1 cut(s)	119
BsrI	ACTGG	1 cut(s)	386
BssECI	CCNNGG	2 cut(s)	107, 312
BssMI	GATC	6 cut(s)	334, 349, 448, 501, 652, 742
BssNI	GRCGYC	1 cut(s)	62
BssSI	CACGAG	1 cut(s)	83
BssT1I	CCWWGG	1 cut(s)	312
Bst2BI	CACGAG	1 cut(s)	83
Bst2UI	CCWGG	1 cut(s)	395
Bst4CI	ACNGT	2 cut(s)	551, 727
BstACI	GRCGYC	1 cut(s)	62
BstBAI	YACGTR	1 cut(s)	373
BstBI	TTCGAA	1 cut(s)	496
BstF5I	GGATG	1 cut(s)	525
BstH2I	RGCGCY	1 cut(s)	103
BstHHI	GCGC	2 cut(s)	82, 102
BstKTI	GATC	6 cut(s)	337, 352, 451, 504, 655, 745
BstMAI	GTCTC	2 cut(s)	18, 333
BstMBI	GATC	6 cut(s)	334, 349, 448, 501, 652, 742
BstMWI	GCNNNNNNNGC	2 cut(s)	88, 97
BstNI	CCWGG	1 cut(s)	395
BstNSI	RCATGY	1 cut(s)	190
BstSCI	CCNGG	2 cut(s)	393, 638
BstSFI	CTRYAG	1 cut(s)	773
BstSLI	GKGCMC	1 cut(s)	388
BstSNI	TACGTA	1 cut(s)	373
BstV1I	GCAGC	1 cut(s)	129
BstV2I	GAAGAC	2 cut(s)	216, 720
BstX2I	RGATCY	2 cut(s)	652, 742
BstXI	CCANNNNNNTGG	1 cut(s)	126
BstYI	RGATCY	2 cut(s)	652, 742
BtsCI	GGATG	1 cut(s)	525
BtsIMutI	CAGTG	2 cut(s)	547, 732
CaiI	CAGNNNCTG	1 cut(s)	394
CciI	TCATGA	4 cut(s)	205, 331, 451, 553
CfoI	GCGC	2 cut(s)	82, 102
Cfr13I	GGNCC	1 cut(s)	584
CseI	GACGC	2 cut(s)	70, 221
Csp6I	GTAC	4 cut(s)	235, 370, 627, 693
CviAII	CATG	6 cut(s)	10, 187, 206, 332, 452, 554
CviJI	RGCY	8 cut(s)	7, 132, 142, 175, 241, 578, 643, 772
CviKI_1	RGCY	8 cut(s)	7, 132, 142, 175, 241, 578, 643, 772
CviQI	GTAC	4 cut(s)	235, 370, 627, 693
DpnI	GATC	6 cut(s)	336, 351, 450, 503, 654, 744
DpnII	GATC	6 cut(s)	334, 349, 448, 501, 652, 742
DraIII	CACNNNGTG	1 cut(s)	437
Ecl136II	GAGCTC	1 cut(s)	7
Eco105I	TACGTA	1 cut(s)	373
Eco130I	CCWWGG	1 cut(s)	312
Eco24I	GRGCYC	1 cut(s)	9
Eco47I	GGWCC	1 cut(s)	584
Eco53kI	GAGCTC	1 cut(s)	7
Eco57I	CTGAAG	2 cut(s)	558, 600
Eco88I	CYCGRG	1 cut(s)	106
EcoICRI	GAGCTC	1 cut(s)	7
EcoO109I	RGGNCCY	1 cut(s)	584
EcoRII	CCWGG	1 cut(s)	393
EcoT14I	CCWWGG	1 cut(s)	312
EcoT38I	GRGCYC	1 cut(s)	9
ErhI	CCWWGG	1 cut(s)	312
FaeI	CATG	6 cut(s)	13, 190, 209, 335, 455, 557
FalI	AAGNNNNNCTT	2 cut(s)	269, 301
FaqI	GGGAC	2 cut(s)	587, 625
FatI	CATG	6 cut(s)	9, 186, 205, 331, 451, 553
FauI	CCCGC	1 cut(s)	45
FbaI	TGATCA	2 cut(s)	334, 448
Fnu4HI	GCNGC	2 cut(s)	41, 143
FokI	GGATG	1 cut(s)	512
FriOI	GRGCYC	1 cut(s)	9
Fsp4HI	GCNGC	2 cut(s)	41, 143
GlaI	GCGC	2 cut(s)	81, 101
GluI	GCNGC	2 cut(s)	41, 143
GsuI	CTGGAG	1 cut(s)	447
HaeII	RGCGCY	1 cut(s)	103
HapII	CCGG	1 cut(s)	639
HgaI	GACGC	2 cut(s)	70, 221
HhaI	GCGC	2 cut(s)	82, 102
Hin1I	GRCGYC	1 cut(s)	62
Hin1II	CATG	6 cut(s)	13, 190, 209, 335, 455, 557
Hin6I	GCGC	2 cut(s)	80, 100
HinP1I	GCGC	2 cut(s)	80, 100
HinfI	GANTC	5 cut(s)	169, 266, 326, 613, 737
HpaII	CCGG	1 cut(s)	639
HphI	GGTGA	1 cut(s)	269
Hpy166II	GTNNAC	1 cut(s)	596
Hpy188I	TCNGA	4 cut(s)	275, 514, 736, 742
Hpy188III	TCNNGA	9 cut(s)	106, 206, 332, 338, 452, 554, 610, 621, 751
Hpy8I	GTNNAC	1 cut(s)	596
Hpy99I	CGWCG	4 cut(s)	49, 64, 70, 161
HpyAV	CCTTC	5 cut(s)	435, 515, 542, 575, 627
HpyCH4III	ACNGT	2 cut(s)	551, 727
HpyCH4IV	ACGT	4 cut(s)	74, 246, 372, 471
HpyCH4V	TGCA	2 cut(s)	414, 730
HpyF10VI	GCNNNNNNNGC	2 cut(s)	88, 97
HpySE526I	ACGT	4 cut(s)	74, 246, 372, 471
Hsp92I	GRCGYC	1 cut(s)	62
Hsp92II	CATG	6 cut(s)	13, 190, 209, 335, 455, 557
HspAI	GCGC	2 cut(s)	80, 100
Ksp22I	TGATCA	2 cut(s)	334, 448
Kzo9I	GATC	6 cut(s)	334, 349, 448, 501, 652, 742
LmnI	GCTCC	3 cut(s)	4, 129, 428
Lsp1109I	GCAGC	1 cut(s)	129
LweI	GCATC	1 cut(s)	224
MaeII	ACGT	4 cut(s)	74, 246, 372, 471
MaeIII	GTNAC	2 cut(s)	257, 377
MalI	GATC	6 cut(s)	336, 351, 450, 503, 654, 744
MboI	GATC	6 cut(s)	334, 349, 448, 501, 652, 742
MboII	GAAGA	6 cut(s)	221, 344, 527, 564, 725, 737
MflI	RGATCY	2 cut(s)	652, 742
MhlI	GDGCHC	3 cut(s)	9, 388, 433
MlyI	GAGTC	2 cut(s)	335, 607
MnlI	CCTC	9 cut(s)	31, 44, 102, 113, 201, 213, 533, 597, 666
MseI	TTAA	2 cut(s)	93, 252
MslI	CAYNNNNRTG	4 cut(s)	124, 360, 470, 552
MspA1I	CMGCKG	2 cut(s)	142, 578
MspI	CCGG	1 cut(s)	639
MspR9I	CCNGG	2 cut(s)	395, 640
Mva1269I	GAATGC	1 cut(s)	414
MvaI	CCWGG	1 cut(s)	395
MwoI	GCNNNNNNNGC	2 cut(s)	88, 97
NciI	CCSGG	1 cut(s)	640
NdeII	GATC	6 cut(s)	334, 349, 448, 501, 652, 742
NlaIII	CATG	6 cut(s)	13, 190, 209, 335, 455, 557
NlaIV	GGNNCC	2 cut(s)	131, 310
NmuCI	GTSAC	2 cut(s)	257, 377
NspI	RCATGY	1 cut(s)	190
NspV	TTCGAA	1 cut(s)	496
OliI	CACNNNNGTG	1 cut(s)	360
PagI	TCATGA	4 cut(s)	205, 331, 451, 553
PctI	GAATGC	1 cut(s)	414
PfeI	GAWTC	3 cut(s)	169, 266, 737
Pfl23II	CGTACG	1 cut(s)	369
PflFI	GACNNNGTC	1 cut(s)	64
PkrI	GCNGC	2 cut(s)	42, 144
PleI	GAGTC	2 cut(s)	334, 607
PpsI	GAGTC	2 cut(s)	334, 607
Ppu21I	YACGTR	1 cut(s)	373
PpuMI	RGGWCCY	1 cut(s)	584
Psp124BI	GAGCTC	1 cut(s)	9
Psp5II	RGGWCCY	1 cut(s)	584
Psp6I	CCWGG	1 cut(s)	393
PspGI	CCWGG	1 cut(s)	393
PspLI	CGTACG	1 cut(s)	369
PspN4I	GGNNCC	2 cut(s)	131, 310
PspPI	GGNCC	1 cut(s)	584
PspPPI	RGGWCCY	1 cut(s)	584
PstNI	CAGNNNCTG	1 cut(s)	394
PsuI	RGATCY	2 cut(s)	652, 742
PsyI	GACNNNGTC	1 cut(s)	64
PvuII	CAGCTG	2 cut(s)	142, 578
RsaI	GTAC	4 cut(s)	236, 371, 628, 694
RsaNI	GTAC	4 cut(s)	235, 370, 627, 693
RseI	CAYNNNNRTG	4 cut(s)	124, 360, 470, 552
SacI	GAGCTC	1 cut(s)	9
SaqAI	TTAA	2 cut(s)	93, 252
SatI	GCNGC	2 cut(s)	41, 143
Sau3AI	GATC	6 cut(s)	334, 349, 448, 501, 652, 742
Sau96I	GGNCC	1 cut(s)	584
ScaI	AGTACT	1 cut(s)	694
SchI	GAGTC	2 cut(s)	335, 607
ScrFI	CCNGG	2 cut(s)	395, 640
SduI	GDGCHC	3 cut(s)	9, 388, 433
SfaNI	GCATC	1 cut(s)	224
SfcI	CTRYAG	1 cut(s)	773
SfuI	TTCGAA	1 cut(s)	496
SinI	GGWCC	1 cut(s)	584
SmiMI	CAYNNNNRTG	4 cut(s)	124, 360, 470, 552
SnaBI	TACGTA	1 cut(s)	373
SsiI	CCGC	3 cut(s)	38, 41, 162
SstI	GAGCTC	1 cut(s)	9
StyD4I	CCNGG	2 cut(s)	393, 638
StyI	CCWWGG	1 cut(s)	312
TaaI	ACNGT	2 cut(s)	551, 727
TaiI	ACGT	4 cut(s)	77, 249, 375, 474
TaqI	TCGA	2 cut(s)	23, 496
TatI	WGTACW	2 cut(s)	626, 692
TauI	GCSGC	1 cut(s)	43
TfiI	GAWTC	3 cut(s)	169, 266, 737
Tru1I	TTAA	2 cut(s)	93, 252
Tru9I	TTAA	2 cut(s)	93, 252
TscAI	CASTG	2 cut(s)	554, 732
TseFI	GTSAC	2 cut(s)	257, 377
TseI	GCWGC	1 cut(s)	142
Tsp45I	GTSAC	2 cut(s)	257, 377
TspDTI	ATGAA	2 cut(s)	222, 675
TspGWI	ACGGA	1 cut(s)	481
TspRI	CASTG	2 cut(s)	554, 732
Tth111I	GACNNNGTC	1 cut(s)	64
VpaK11BI	GGWCC	1 cut(s)	584
XceI	RCATGY	1 cut(s)	190
ZrmI	AGTACT	1 cut(s)	694

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.