Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006621

Physical Location & Seq

Genomic Coordinates Forward (+)

36549032 .. 36551654

Gene Structure 2623 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1285900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 777 bp

ATGGACCTCATGTCTCATTCCTCCACCTTCCTCCTCCCTACGATCCCGCCCAACCAAACGCCGTCTCCCACCTACGCGCTCGTCTTGCTCAACCAGAGCCTCCCGAGATTCGCTCCTTTGCTCTGGAACCACGCAGCACAACTACGTATGTGTGCCGATGGCGGTGCCAATCGCGTTTACGACGACATGCCCCTTCTGCTGCCGCACGAAAACGCCGTAGATGTTCGGAAAAGGTACAAGCCCGATGTTATTAAAGGCGACATGGATTCAATCCGAAAGGATGTCTTGGAATTTTATGCTAGTATGGGAACTCAAATTATTAATGAATCTGAGGATCAGGACACAACCGATTTGCATAAGTGTATAACATACATATGTGACTCGGCACCAAACCTAGATAAGTCTAATCTATGCATTCTGGTTGCTGGGGCACTTGGTGGACGGTTTGACCACGAGATTGGAAACATCAACGTGTTATGTCGATTCTCAAACATTCGAATAATTCTCATATCTGATGATTGCCTCATCCATCTTCTTCCAAGAACACACCGCCATGAGATCCATATTCAGTCTTCTGTTGAAGGCCCTCATTGTGGACTCATACCCATTGGGATGCCATCTGGAAGTACTACAACCACTGGACTTCAATGGGATCTCGCTGAAACGGAGATGAGGTTTGGTGGTCTGATAAGTACATCAAATATCGTCAATGGAGAGAAAATAACAGTGCAGTCAGATTCAGATCTTCTTTGGACTATAAGCATTAAAAAGATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004788 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009229 GO:0009987 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0034641 GO:0042357 GO:0042723 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0051186 GO:0051188 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

TPK_B1_binding TPK_catalytic

Protein Analysis

259

Amino Acids

28.65

Weight (kDa)

5.5

Isoelectric Point (pI)

53.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPK_catalytic	PF04263	41 - 168	6.9e-38	Thiamin pyrophosphokinase, catalytic domain
TPK_B1_binding	PF04265	184 - 250	5.5e-22	Thiamin pyrophosphokinase, vitamin B1 binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006621)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02880 AT1G02880 AT1G02880 AT2G44750 AT2G44750
fragaria_vesca	FvH4_2g25500 FvH4_2g25500
malus_domestica	MD02G1044000.v1.1 MD02G1044100.v1.1 MD03G1285900.v1.1 MD06G1023200.v1.1 MD06G1023300.v1.1 MD11G1034700.v1.1 MD11G1305700.v1.1
prunus_persica	Prupe.8G259100_v2.0.a1
pyrus_communis	pycom03g22660 pycom11g26750
rosa_chinensis	RchiOBHm_Chr6g0293711
rosa_laevigata	RLG00000011930
rosa_roxburghii	Rroxscaffold_7G00173970
rosa_rugosa	Rorug06G0237500
rosa_samantha	Rh6AG350300 Rh6BG357800 Rh6CG364600 Rh6DG351500
rosa_wichuraiana	Rw6G030500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	164, 385
AccII	CGCG	2 cut(s)	77, 174
AciI	CCGC	4 cut(s)	47, 162, 203, 550
AclWI	GGATC	4 cut(s)	37, 342, 553, 660
AcsI	RAATTY	1 cut(s)	290
AdeI	CACNNNGTG	1 cut(s)	437
AfaI	GTAC	3 cut(s)	236, 628, 694
AfiI	CCNNNNNNNGG	1 cut(s)	593
AflIII	ACRYGT	1 cut(s)	471
AgsI	TTSAA	3 cut(s)	270, 581, 647
AhdI	GACNNNNNGTC	1 cut(s)	10
Alw26I	GTCTC	2 cut(s)	18, 69
AlwI	GGATC	4 cut(s)	37, 342, 553, 660
Ama87I	CYCGRG	1 cut(s)	103
AoxI	GGCC	1 cut(s)	583
ApeKI	GCWGC	2 cut(s)	134, 199
ApoI	RAATTY	1 cut(s)	290
AseI	ATTAAT	1 cut(s)	321
AspLEI	GCGC	1 cut(s)	79
AspS9I	GGNCC	2 cut(s)	4, 584
AsuII	TTCGAA	1 cut(s)	496
AvaI	CYCGRG	1 cut(s)	103
AvaII	GGWCC	1 cut(s)	4
BaeGI	GKGCMC	1 cut(s)	433
BanI	GGYRCC	2 cut(s)	164, 385
BauI	CACGAG	1 cut(s)	452
BbsI	GAAGAC	1 cut(s)	564
BbvI	GCAGC	2 cut(s)	146, 186
BccI	CCATC	3 cut(s)	152, 537, 625
BceAI	ACGGC	2 cut(s)	46, 200
BcgI	CGANNNNNNTGC	2 cut(s)	146, 180
BcoDI	GTCTC	2 cut(s)	18, 69
BfaI	CTAG	2 cut(s)	300, 395
BglII	AGATCT	1 cut(s)	742
BisI	GCNGC	3 cut(s)	135, 200, 203
BlsI	GCNGC	3 cut(s)	136, 201, 204
BmcAI	AGTACT	1 cut(s)	628
Bme18I	GGWCC	1 cut(s)	4
BmeRI	GACNNNNNGTC	1 cut(s)	10
BmeT110I	CYCGRG	1 cut(s)	103
BmgT120I	GGNCC	2 cut(s)	4, 584
BmiI	GGNNCC	3 cut(s)	128, 166, 387
BmsI	GCATC	1 cut(s)	603
BpiI	GAAGAC	1 cut(s)	564
BplI	GAGNNNNNCTC	2 cut(s)	97, 129
Bpu14I	TTCGAA	1 cut(s)	496
BsaAI	YACGTR	1 cut(s)	146
BsaBI	GATNNNNATC	1 cut(s)	741
BsaXI	ACNNNNNCTCC	4 cut(s)	49, 79, 659, 689
Bsc4I	CCNNNNNNNGG	1 cut(s)	593
Bse1I	ACTGG	1 cut(s)	643
Bse8I	GATNNNNATC	1 cut(s)	741
BseGI	GGATG	3 cut(s)	286, 525, 618
BseJI	GATNNNNATC	1 cut(s)	741
BseLI	CCNNNNNNNGG	1 cut(s)	593
BseMII	CTCAG	1 cut(s)	321
BseNI	ACTGG	1 cut(s)	643
BseRI	GAGGAG	1 cut(s)	23
BseSI	GKGCMC	1 cut(s)	433
BseXI	GCAGC	2 cut(s)	146, 186
BseYI	CCCAGC	1 cut(s)	425
BsgI	GTGCAG	1 cut(s)	749
Bsh1236I	CGCG	2 cut(s)	77, 174
BshFI	GGCC	1 cut(s)	585
BshNI	GGYRCC	2 cut(s)	164, 385
BsiHKCI	CYCGRG	1 cut(s)	103
BslI	CCNNNNNNNGG	1 cut(s)	593
BsmAI	GTCTC	2 cut(s)	18, 69
BsmBI	CGTCTC	1 cut(s)	69
BsmI	GAATGC	1 cut(s)	414
BsnI	GGCC	1 cut(s)	585
BsoBI	CYCGRG	1 cut(s)	103
Bsp119I	TTCGAA	1 cut(s)	496
Bsp1286I	GDGCHC	1 cut(s)	433
Bsp143I	GATC	5 cut(s)	42, 334, 558, 652, 742
BspACI	CCGC	4 cut(s)	47, 162, 203, 550
BspANI	GGCC	1 cut(s)	585
BspCNI	CTCAG	1 cut(s)	322
BspFNI	CGCG	2 cut(s)	77, 174
BspLI	GGNNCC	3 cut(s)	128, 166, 387
BspPI	GGATC	4 cut(s)	37, 342, 553, 660
BspT104I	TTCGAA	1 cut(s)	496
BspT107I	GGYRCC	2 cut(s)	164, 385
BsrI	ACTGG	1 cut(s)	643
BssMI	GATC	5 cut(s)	42, 334, 558, 652, 742
BssSI	CACGAG	1 cut(s)	452
Bst2BI	CACGAG	1 cut(s)	452
Bst4CI	ACNGT	2 cut(s)	444, 727
BstBAI	YACGTR	1 cut(s)	146
BstBI	TTCGAA	1 cut(s)	496
BstDEI	CTNAG	1 cut(s)	330
BstF5I	GGATG	3 cut(s)	286, 525, 618
BstFNI	CGCG	2 cut(s)	77, 174
BstHHI	GCGC	1 cut(s)	79
BstKTI	GATC	5 cut(s)	45, 337, 561, 655, 745
BstMAI	GTCTC	2 cut(s)	18, 69
BstMBI	GATC	5 cut(s)	42, 334, 558, 652, 742
BstMWI	GCNNNNNNNGC	2 cut(s)	85, 196
BstNSI	RCATGY	1 cut(s)	190
BstSLI	GKGCMC	1 cut(s)	433
BstSNI	TACGTA	1 cut(s)	146
BstUI	CGCG	2 cut(s)	77, 174
BstV1I	GCAGC	2 cut(s)	146, 186
BstV2I	GAAGAC	1 cut(s)	564
BstX2I	RGATCY	3 cut(s)	558, 652, 742
BstXI	CCANNNNNNTGG	1 cut(s)	458
BstYI	RGATCY	3 cut(s)	558, 652, 742
BsuRI	GGCC	1 cut(s)	585
BtsCI	GGATG	3 cut(s)	286, 525, 618
BtsIMutI	CAGTG	2 cut(s)	636, 732
CfoI	GCGC	1 cut(s)	79
Cfr13I	GGNCC	2 cut(s)	4, 584
Csp6I	GTAC	3 cut(s)	235, 627, 693
CviAII	CATG	4 cut(s)	10, 187, 262, 554
CviJI	RGCY	3 cut(s)	99, 241, 585
CviKI_1	RGCY	3 cut(s)	99, 241, 585
CviQI	GTAC	3 cut(s)	235, 627, 693
DdeI	CTNAG	1 cut(s)	330
DpnI	GATC	5 cut(s)	44, 336, 560, 654, 744
DpnII	GATC	5 cut(s)	42, 334, 558, 652, 742
DraIII	CACNNNGTG	1 cut(s)	437
DriI	GACNNNNNGTC	1 cut(s)	10
Eam1105I	GACNNNNNGTC	1 cut(s)	10
Eco105I	TACGTA	1 cut(s)	146
Eco47I	GGWCC	1 cut(s)	4
Eco88I	CYCGRG	1 cut(s)	103
EcoO109I	RGGNCCY	1 cut(s)	584
EcoT22I	ATGCAT	1 cut(s)	416
Esp3I	CGTCTC	1 cut(s)	69
FaeI	CATG	4 cut(s)	13, 190, 265, 557
FalI	AAGNNNNNCTT	2 cut(s)	269, 301
FatI	CATG	4 cut(s)	9, 186, 261, 553
FauI	CCCGC	1 cut(s)	54
FauNDI	CATATG	1 cut(s)	374
Fnu4HI	GCNGC	3 cut(s)	135, 200, 203
FokI	GGATG	3 cut(s)	293, 512, 625
Fsp4HI	GCNGC	3 cut(s)	135, 200, 203
FspBI	CTAG	2 cut(s)	300, 395
GlaI	GCGC	1 cut(s)	78
GluI	GCNGC	3 cut(s)	135, 200, 203
GsaI	CCCAGC	1 cut(s)	429
HaeIII	GGCC	1 cut(s)	585
HhaI	GCGC	1 cut(s)	79
Hin1II	CATG	4 cut(s)	13, 190, 265, 557
Hin6I	GCGC	1 cut(s)	77
HinP1I	GCGC	1 cut(s)	77
HinfI	GANTC	7 cut(s)	108, 266, 326, 380, 483, 597, 737
Hpy166II	GTNNAC	3 cut(s)	178, 440, 596
Hpy188I	TCNGA	7 cut(s)	228, 275, 331, 514, 687, 736, 742
Hpy188III	TCNNGA	4 cut(s)	103, 124, 338, 621
Hpy8I	GTNNAC	3 cut(s)	178, 440, 596
Hpy99I	CGWCG	1 cut(s)	185
HpyAV	CCTTC	3 cut(s)	37, 203, 575
HpyCH4III	ACNGT	2 cut(s)	444, 727
HpyCH4IV	ACGT	2 cut(s)	145, 471
HpyCH4V	TGCA	3 cut(s)	355, 414, 730
HpyF10VI	GCNNNNNNNGC	2 cut(s)	85, 196
HpyF3I	CTNAG	1 cut(s)	330
HpySE526I	ACGT	2 cut(s)	145, 471
Hsp92II	CATG	4 cut(s)	13, 190, 265, 557
HspAI	GCGC	1 cut(s)	77
Kzo9I	GATC	5 cut(s)	42, 334, 558, 652, 742
LmnI	GCTCC	1 cut(s)	118
LpnPI	CCDG	7 cut(s)	107, 109, 323, 404, 411, 606, 624
Lsp1109I	GCAGC	2 cut(s)	146, 186
LweI	GCATC	1 cut(s)	603
MaeI	CTAG	2 cut(s)	300, 395
MaeII	ACGT	2 cut(s)	145, 471
MaeIII	GTNAC	1 cut(s)	377
MalI	GATC	5 cut(s)	44, 336, 560, 654, 744
MboI	GATC	5 cut(s)	42, 334, 558, 652, 742
MboII	GAAGA	4 cut(s)	524, 527, 564, 737
MflI	RGATCY	3 cut(s)	558, 652, 742
MhlI	GDGCHC	1 cut(s)	433
MluCI	AATT	3 cut(s)	290, 315, 501
MlyI	GAGTC	2 cut(s)	374, 591
MnlI	CCTC	9 cut(s)	17, 31, 41, 44, 110, 325, 533, 597, 666
Mph1103I	ATGCAT	1 cut(s)	416
MseI	TTAA	3 cut(s)	252, 321, 765
MslI	CAYNNNNRTG	4 cut(s)	373, 470, 552, 611
Mva1269I	GAATGC	1 cut(s)	414
MvnI	CGCG	2 cut(s)	77, 174
MwoI	GCNNNNNNNGC	2 cut(s)	85, 196
NdeI	CATATG	1 cut(s)	374
NdeII	GATC	5 cut(s)	42, 334, 558, 652, 742
NlaIII	CATG	4 cut(s)	13, 190, 265, 557
NlaIV	GGNNCC	3 cut(s)	128, 166, 387
NmeAIII	GCCGAG	1 cut(s)	362
NmuCI	GTSAC	1 cut(s)	377
NsiI	ATGCAT	1 cut(s)	416
NspI	RCATGY	1 cut(s)	190
NspV	TTCGAA	1 cut(s)	496
PcsI	WCGNNNNNNNCGW	1 cut(s)	213
PctI	GAATGC	1 cut(s)	414
PfeI	GAWTC	5 cut(s)	108, 266, 326, 483, 737
PkrI	GCNGC	3 cut(s)	136, 201, 204
PleI	GAGTC	2 cut(s)	374, 591
PpsI	GAGTC	2 cut(s)	374, 591
Ppu21I	YACGTR	1 cut(s)	146
PshBI	ATTAAT	1 cut(s)	321
PspFI	CCCAGC	1 cut(s)	425
PspN4I	GGNNCC	3 cut(s)	128, 166, 387
PspPI	GGNCC	2 cut(s)	4, 584
PsuI	RGATCY	3 cut(s)	558, 652, 742
RsaI	GTAC	3 cut(s)	236, 628, 694
RsaNI	GTAC	3 cut(s)	235, 627, 693
RseI	CAYNNNNRTG	4 cut(s)	373, 470, 552, 611
SaqAI	TTAA	3 cut(s)	252, 321, 765
SatI	GCNGC	3 cut(s)	135, 200, 203
Sau3AI	GATC	5 cut(s)	42, 334, 558, 652, 742
Sau96I	GGNCC	2 cut(s)	4, 584
ScaI	AGTACT	1 cut(s)	628
SchI	GAGTC	2 cut(s)	374, 591
SduI	GDGCHC	1 cut(s)	433
SetI	ASST	8 cut(s)	9, 29, 74, 148, 236, 396, 474, 677
SfaNI	GCATC	1 cut(s)	603
SfuI	TTCGAA	1 cut(s)	496
SinI	GGWCC	1 cut(s)	4
SmiMI	CAYNNNNRTG	4 cut(s)	373, 470, 552, 611
SnaBI	TACGTA	1 cut(s)	146
Sse9I	AATT	3 cut(s)	290, 315, 501
SsiI	CCGC	4 cut(s)	47, 162, 203, 550
SspMI	CTAG	2 cut(s)	300, 395
TaaI	ACNGT	2 cut(s)	444, 727
TaiI	ACGT	2 cut(s)	148, 474
TaqI	TCGA	2 cut(s)	481, 496
TasI	AATT	3 cut(s)	290, 315, 501
TatI	WGTACW	2 cut(s)	626, 692
TauI	GCSGC	1 cut(s)	205
TfiI	GAWTC	5 cut(s)	108, 266, 326, 483, 737
Tru1I	TTAA	3 cut(s)	252, 321, 765
Tru9I	TTAA	3 cut(s)	252, 321, 765
TscAI	CASTG	2 cut(s)	643, 732
TseFI	GTSAC	1 cut(s)	377
TseI	GCWGC	2 cut(s)	134, 199
Tsp45I	GTSAC	1 cut(s)	377
TspDTI	ATGAA	1 cut(s)	339
TspGWI	ACGGA	1 cut(s)	680
TspRI	CASTG	2 cut(s)	643, 732
VpaK11BI	GGWCC	1 cut(s)	4
VspI	ATTAAT	1 cut(s)	321
XapI	RAATTY	1 cut(s)	290
XceI	RCATGY	1 cut(s)	190
XspI	CTAG	2 cut(s)	300, 395
ZrmI	AGTACT	1 cut(s)	628
Zsp2I	ATGCAT	1 cut(s)	416

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.